pycom14g16890

EXOIII

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0004362
Physical Location & Seq
Forward (+)
19041180 .. 19043785
2606 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1497 bp
ATGGCGAGTCGGAGCGAGGATCGGTTCGAAATAGCATTCTTCGACGTGGAGACGACGGTGCCGACCCGACCCGGACAGGGCTTCACCATCTTGGAATTCGGTTCGATTCTCGTTTGCCCTAGGAAGCTGGTGGAGCTCGAGAGCTACTCCACCTTGATTCGACCTGCTGACCTCTCCGCCATCAATTCCCTGTCCGTCCGTAGCAACGGCATTACCCGCGACGCCGTCATTGCCGCCCCTTCTTTTCAAGACGTCGCCGATACGGTCTACGACATTCTCCACGAACGTATATGGGCTGGTCACAACATTCTGAGGTTTGATTGTTATCGGATTCGGGAGGCTTTCGCACGCATTGGTCGGCCTGCACCAGAACCGAAAGGTACCATTGATTCATTGGCATTGTTGACACAGCGGTTCGGAAGGAGAGCTGGTAACATGAAGATGGCCTCCCTTGCAACCTATTTCGGGCTTGGACAGCAGACACACAGGAGTTTGGATGACGTTCGAATGAATCTTGAAGTGCTGAAGTATTGTGCAACCGTGTTACTCTTGGAGTCGAGCCTCCCAGACATATTCACAGAGAATAGTTGGGTTTCTCCAAATGCTACCACAAGAAGTCGTAGTGACGGGAAATCATCTGCTGAGGGGTCTCGCATAAACATGAATACTGAGCATGCAAATCAAATAACAGGGGAGAATCATCCAATAACATCTCTTAGGACTAAAGACACTGAAGAAGTCTCTAATCTGATTGAATCTAGCACCGCTCGACCAGATCCCTTTGACATGGGCCCACTTAACGATGAAGTGATGAAAGAGCTGATTCAGCCAGACATCGCCATGGTAGAAATACCTTTGAAAGAGTCTGTCGAGAGTTCTTCCCCTTTTGCTGCATCGGGGAGTTCCAATGCCACTTTGGACTTTTTGCAGCCTGATGAAATCTCTATTCCTTCTATCTGTGCTTCCCTTGTTCCGTTGTATCGCGGGAGTCAGAGGATAAAGTTGTTACACAAAGATGTAAGTTTGCAGCTTTGCTGTAGGCATATGAAATTGCGGTTTGGAATCAATGGAAAGTTTTTTGATCATGCTGGCCGGCCACGATTGAATATTGTTTGTTACGCATCACAAAATTTGTGCAAAGTTCTTGATGCATGTGATGGCATTGCACAAAAGTTGTCTGTGGATTCCGGTAGCAGCTCCGAATGGAAGCCTGTTGTGATCCGAAAAGAGGGTTTCAATAACTATCCTACAGTGAGATTACACATACAGACAGCAGTATGTGGGGATATTGCAATATACGCCACAGAGATATATCAAAAAGAGCCTTCTGGTGCCGAGGAGAGGCTTGTATTCACCAAGTTTGATGCTTCAGCACTTAGCACCCTTTTCAAACCGCAGACTTTTATGGATGCATTCTTCTCCTTGGATCCGTATGACTATCAGCAGAGTGCAGGCATTCGGTTGGTGGCAAAGAAATTGATTATTCATTCCAATTGA

Protein Analysis

499

Amino Acids

55.33

Weight (kDa)

5.99

Isoelectric Point (pI)

47.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_T PF00929 13 - 175 1.3e-26 Exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 255
Acc36I ACCTGC 1 cut(s) 172
Acc65I GGTACC 1 cut(s) 380
AccB1I GGYRCC 3 cut(s) 58, 380, 1331
AccBSI CCGCTC 1 cut(s) 767
AccI GTMKAC 1 cut(s) 267
AccII CGCG 2 cut(s) 219, 984
AciI CCGC 8 cut(s) 177, 217, 234, 412, 765, 984, 1054, 1394
AclWI GGATC 5 cut(s) 27, 770, 1213, 1421, 1434
AcoI YGGCCR 2 cut(s) 1090, 1094
AcsI RAATTY 2 cut(s) 95, 1129
AcuI CTGAAG 3 cut(s) 545, 753, 1353
AcyI GRCGYC 2 cut(s) 222, 252
AfaI GTAC 1 cut(s) 382
AfiI CCNNNNNNNGG 4 cut(s) 77, 465, 1228, 1341
AgsI TTSAA 7 cut(s) 248, 518, 755, 859, 1105, 1237, 1390
AjiI CACGTC 1 cut(s) 46
AluBI AGCT 7 cut(s) 127, 136, 144, 428, 820, 1030, 1197
AluI AGCT 7 cut(s) 127, 136, 144, 428, 820, 1030, 1197
Alw21I GWGCWC 1 cut(s) 138
Alw26I GTCTC 3 cut(s) 44, 654, 745
AlwI GGATC 5 cut(s) 27, 770, 1213, 1421, 1434
Ama87I CYCGRG 1 cut(s) 137
AoxI GGCC 5 cut(s) 359, 444, 790, 1090, 1094
ApaI GGGCCC 1 cut(s) 794
ApeKI GCWGC 4 cut(s) 890, 928, 1027, 1194
ApoI RAATTY 2 cut(s) 95, 1129
Asp718I GGTACC 1 cut(s) 380
AspA2I CCTAGG 1 cut(s) 119
AspS9I GGNCC 2 cut(s) 790, 791
AsuC2I CCSGG 1 cut(s) 72
AsuHPI GGTGA 2 cut(s) 76, 1345
AsuII TTCGAA 2 cut(s) 27, 505
AvaI CYCGRG 1 cut(s) 137
AvrII CCTAGG 1 cut(s) 119
BaeGI GKGCMC 1 cut(s) 794
BamHI GGATCC 1 cut(s) 1426
BanI GGYRCC 3 cut(s) 58, 380, 1331
BanII GRGCYC 2 cut(s) 138, 794
Bbv12I GWGCWC 1 cut(s) 138
BbvCI CCTCAGC 1 cut(s) 642
BbvI GCAGC 4 cut(s) 877, 940, 1039, 1206
BccI CCATC 4 cut(s) 95, 188, 436, 1151
BceAI ACGGC 2 cut(s) 209, 223
BclI TGATCA 1 cut(s) 1081
BcnI CCSGG 1 cut(s) 72
BcoDI GTCTC 3 cut(s) 44, 654, 745
BfaI CTAG 2 cut(s) 120, 759
BfmI CTRYAG 2 cut(s) 1036, 1248
BfuAI ACCTGC 1 cut(s) 172
BisI GCNGC 5 cut(s) 234, 891, 929, 1028, 1195
BlnI CCTAGG 1 cut(s) 119
BlsI GCNGC 5 cut(s) 235, 892, 930, 1029, 1196
Bme1390I CCNGG 1 cut(s) 72
BmeT110I CYCGRG 1 cut(s) 137
BmgBI CACGTC 1 cut(s) 46
BmgT120I GGNCC 2 cut(s) 790, 791
BmiI GGNNCC 5 cut(s) 60, 382, 792, 1333, 1428
BmrFI CCNGG 1 cut(s) 72
BmsI GCATC 5 cut(s) 902, 1130, 1138, 1354, 1399
BplI GAGNNNNNCTC 2 cut(s) 131, 163
Bpu10I CCTNAGC 1 cut(s) 642
Bpu14I TTCGAA 2 cut(s) 27, 505
BpuMI CCSGG 1 cut(s) 72
BsaBI GATNNNNATC 1 cut(s) 324
BsaHI GRCGYC 2 cut(s) 222, 252
BsaI GGTCTC 1 cut(s) 654
BsaJI CCNNGG 4 cut(s) 119, 840, 1335, 1422
BsaWI WCCGGW 1 cut(s) 1187
BsaXI ACNNNNNCTCC 2 cut(s) 415, 445
Bsc4I CCNNNNNNNGG 4 cut(s) 77, 465, 1228, 1341
Bse118I RCCGGY 1 cut(s) 1092
Bse3DI GCAATG 2 cut(s) 228, 1161
Bse8I GATNNNNATC 1 cut(s) 324
BseDI CCNNGG 4 cut(s) 119, 840, 1335, 1422
BseGI GGATG 3 cut(s) 502, 700, 1414
BseJI GATNNNNATC 1 cut(s) 324
BseLI CCNNNNNNNGG 4 cut(s) 77, 465, 1228, 1341
BseMI GCAATG 2 cut(s) 228, 1161
BseMII CTCAG 3 cut(s) 302, 633, 660
BseRI GAGGAG 1 cut(s) 1352
BseSI GKGCMC 1 cut(s) 794
BseXI GCAGC 4 cut(s) 877, 940, 1039, 1206
BsgI GTGCAG 2 cut(s) 348, 1470
Bsh1236I CGCG 2 cut(s) 219, 984
BshFI GGCC 5 cut(s) 361, 446, 792, 1092, 1096
BshNI GGYRCC 3 cut(s) 58, 380, 1331
BsiHKAI GWGCWC 1 cut(s) 138
BsiHKCI CYCGRG 1 cut(s) 137
BsiSI CCGG 3 cut(s) 72, 1093, 1188
BslI CCNNNNNNNGG 4 cut(s) 77, 465, 1228, 1341
BsmAI GTCTC 3 cut(s) 44, 654, 745
BsmBI CGTCTC 1 cut(s) 44
BsmI GAATGC 3 cut(s) 35, 1412, 1455
BsnI GGCC 5 cut(s) 361, 446, 792, 1092, 1096
Bso31I GGTCTC 1 cut(s) 654
BsoBI CYCGRG 1 cut(s) 137
Bsp119I TTCGAA 2 cut(s) 27, 505
Bsp120I GGGCCC 1 cut(s) 790
Bsp1286I GDGCHC 2 cut(s) 138, 794
Bsp143I GATC 5 cut(s) 19, 775, 1081, 1218, 1426
Bsp19I CCATGG 1 cut(s) 840
BspACI CCGC 8 cut(s) 177, 217, 234, 412, 765, 984, 1054, 1394
BspANI GGCC 5 cut(s) 361, 446, 792, 1092, 1096
BspCNI CTCAG 3 cut(s) 303, 634, 661
BspFNI CGCG 2 cut(s) 219, 984
BspLI GGNNCC 5 cut(s) 60, 382, 792, 1333, 1428
BspMI ACCTGC 1 cut(s) 172
BspPI GGATC 5 cut(s) 27, 770, 1213, 1421, 1434
BspT104I TTCGAA 2 cut(s) 27, 505
BspT107I GGYRCC 3 cut(s) 58, 380, 1331
BspTNI GGTCTC 1 cut(s) 654
BsrBI CCGCTC 1 cut(s) 767
BsrDI GCAATG 2 cut(s) 228, 1161
BsrFI RCCGGY 1 cut(s) 1092
BssAI RCCGGY 1 cut(s) 1092
BssECI CCNNGG 4 cut(s) 119, 840, 1335, 1422
BssMI GATC 5 cut(s) 19, 775, 1081, 1218, 1426
BssNI GRCGYC 2 cut(s) 222, 252
BssT1I CCWWGG 3 cut(s) 119, 840, 1422
Bst4CI ACNGT 4 cut(s) 58, 265, 541, 1252
BstACI GRCGYC 2 cut(s) 222, 252
BstBI TTCGAA 2 cut(s) 27, 505
BstC8I GCNNGC 6 cut(s) 349, 363, 675, 1090, 1094, 1453
BstDEI CTNAG 5 cut(s) 311, 642, 669, 716, 1376
BstDSI CCRYGG 1 cut(s) 840
BstF5I GGATG 3 cut(s) 502, 700, 1414
BstFNI CGCG 2 cut(s) 219, 984
BstKTI GATC 5 cut(s) 22, 778, 1084, 1221, 1429
BstMAI GTCTC 3 cut(s) 44, 654, 745
BstMBI GATC 5 cut(s) 19, 775, 1081, 1218, 1426
BstMWI GCNNNNNNNGC 7 cut(s) 133, 216, 230, 452, 475, 826, 1298
BstNSI RCATGY 2 cut(s) 677, 1155
BstSCI CCNGG 1 cut(s) 70
BstSFI CTRYAG 2 cut(s) 1036, 1248
BstSLI GKGCMC 1 cut(s) 794
BstUI CGCG 2 cut(s) 219, 984
BstV1I GCAGC 4 cut(s) 877, 940, 1039, 1206
BstX2I RGATCY 2 cut(s) 775, 1426
BstYI RGATCY 2 cut(s) 775, 1426
BsuRI GGCC 5 cut(s) 361, 446, 792, 1092, 1096
BtgI CCRYGG 1 cut(s) 840
BtgZI GCGATG 1 cut(s) 820
BtrI CACGTC 1 cut(s) 46
BtsCI GGATG 3 cut(s) 502, 700, 1414
BtsIMutI CAGTG 2 cut(s) 729, 1257
BveI ACCTGC 1 cut(s) 172
Cac8I GCNNGC 6 cut(s) 349, 363, 675, 1090, 1094, 1453
Cfr10I RCCGGY 1 cut(s) 1092
Cfr13I GGNCC 2 cut(s) 790, 791
CseI GACGC 1 cut(s) 230
Csp6I GTAC 1 cut(s) 381
CviAII CATG 7 cut(s) 436, 661, 674, 787, 841, 1085, 1152
CviQI GTAC 1 cut(s) 381
DdeI CTNAG 5 cut(s) 311, 642, 669, 716, 1376
DpnI GATC 5 cut(s) 21, 777, 1083, 1220, 1428
DpnII GATC 5 cut(s) 19, 775, 1081, 1218, 1426
EaeI YGGCCR 2 cut(s) 1090, 1094
EciI GGCGGA 1 cut(s) 166
Ecl136II GAGCTC 1 cut(s) 136
Eco130I CCWWGG 3 cut(s) 119, 840, 1422
Eco24I GRGCYC 2 cut(s) 138, 794
Eco31I GGTCTC 1 cut(s) 654
Eco53kI GAGCTC 1 cut(s) 136
Eco57I CTGAAG 3 cut(s) 545, 753, 1353
Eco88I CYCGRG 1 cut(s) 137
EcoICRI GAGCTC 1 cut(s) 136
EcoRI GAATTC 1 cut(s) 95
EcoT14I CCWWGG 3 cut(s) 119, 840, 1422
EcoT22I ATGCAT 2 cut(s) 1153, 1414
EcoT38I GRGCYC 2 cut(s) 138, 794
ErhI CCWWGG 3 cut(s) 119, 840, 1422
Esp3I CGTCTC 1 cut(s) 44
FaeI CATG 7 cut(s) 439, 664, 677, 790, 844, 1088, 1155
FatI CATG 7 cut(s) 435, 660, 673, 786, 840, 1084, 1151
FauI CCCGC 2 cut(s) 224, 977
FauNDI CATATG 1 cut(s) 1044
FbaI TGATCA 1 cut(s) 1081
FblI GTMKAC 1 cut(s) 267
Fnu4HI GCNGC 5 cut(s) 234, 891, 929, 1028, 1195
FokI GGATG 3 cut(s) 509, 687, 1421
FriOI GRGCYC 2 cut(s) 138, 794
FseI GGCCGGCC 1 cut(s) 1096
Fsp4HI GCNGC 5 cut(s) 234, 891, 929, 1028, 1195
FspBI CTAG 2 cut(s) 120, 759
GluI GCNGC 5 cut(s) 234, 891, 929, 1028, 1195
HaeIII GGCC 5 cut(s) 361, 446, 792, 1092, 1096
HapII CCGG 3 cut(s) 72, 1093, 1188
HgaI GACGC 1 cut(s) 230
Hin1I GRCGYC 2 cut(s) 222, 252
Hin1II CATG 7 cut(s) 439, 664, 677, 790, 844, 1088, 1155
HincII GTYRAC 1 cut(s) 405
HindII GTYRAC 1 cut(s) 405
HpaII CCGG 3 cut(s) 72, 1093, 1188
HphI GGTGA 2 cut(s) 76, 1345
Hpy166II GTNNAC 2 cut(s) 268, 405
Hpy188I TCNGA 8 cut(s) 12, 312, 330, 419, 750, 993, 1201, 1223
Hpy188III TCNNGA 6 cut(s) 139, 248, 335, 515, 871, 1145
Hpy8I GTNNAC 2 cut(s) 268, 405
Hpy99I CGWCG 4 cut(s) 47, 58, 224, 257
HpyAV CCTTC 4 cut(s) 249, 414, 960, 1335
HpyCH4III ACNGT 4 cut(s) 58, 265, 541, 1252
HpyCH4IV ACGT 4 cut(s) 45, 252, 286, 501
HpyF10VI GCNNNNNNNGC 7 cut(s) 133, 216, 230, 452, 475, 826, 1298
HpyF3I CTNAG 5 cut(s) 311, 642, 669, 716, 1376
HpySE526I ACGT 4 cut(s) 45, 252, 286, 501
Hsp92I GRCGYC 2 cut(s) 222, 252
Hsp92II CATG 7 cut(s) 439, 664, 677, 790, 844, 1088, 1155
KpnI GGTACC 1 cut(s) 384
KroI GCCGGC 1 cut(s) 1092
KroNI GCCGGC 1 cut(s) 1094
Ksp22I TGATCA 1 cut(s) 1081
Kzo9I GATC 5 cut(s) 19, 775, 1081, 1218, 1426
LmnI GCTCC 3 cut(s) 12, 133, 1202
Lsp1109I GCAGC 4 cut(s) 877, 940, 1039, 1206
LweI GCATC 5 cut(s) 902, 1130, 1138, 1354, 1399
MaeI CTAG 2 cut(s) 120, 759
MaeII ACGT 4 cut(s) 45, 252, 286, 501
MaeIII GTNAC 6 cut(s) 299, 431, 543, 623, 1005, 1115
MalI GATC 5 cut(s) 21, 777, 1083, 1220, 1428
MbiI CCGCTC 1 cut(s) 767
MboI GATC 5 cut(s) 19, 775, 1081, 1218, 1426
MboII GAAGA 5 cut(s) 31, 451, 746, 870, 1408
MfeI CAATTG 1 cut(s) 1492
MflI RGATCY 2 cut(s) 775, 1426
MhlI GDGCHC 2 cut(s) 138, 794
MluCI AATT 6 cut(s) 95, 184, 1049, 1129, 1475, 1492
MlyI GAGTC 4 cut(s) 16, 563, 872, 997
Mph1103I ATGCAT 2 cut(s) 1153, 1414
MroNI GCCGGC 1 cut(s) 1092
MseI TTAA 1 cut(s) 798
MslI CAYNNNNRTG 4 cut(s) 440, 659, 839, 1014
MspA1I CMGCKG 1 cut(s) 412
MspI CCGG 3 cut(s) 72, 1093, 1188
MspR9I CCNGG 1 cut(s) 72
MunI CAATTG 1 cut(s) 1492
Mva1269I GAATGC 3 cut(s) 35, 1412, 1455
MvnI CGCG 2 cut(s) 219, 984
MwoI GCNNNNNNNGC 7 cut(s) 133, 216, 230, 452, 475, 826, 1298
NaeI GCCGGC 1 cut(s) 1094
NciI CCSGG 1 cut(s) 72
NcoI CCATGG 1 cut(s) 840
NdeI CATATG 1 cut(s) 1044
NdeII GATC 5 cut(s) 19, 775, 1081, 1218, 1426
NgoMIV GCCGGC 1 cut(s) 1092
NlaIII CATG 7 cut(s) 439, 664, 677, 790, 844, 1088, 1155
NlaIV GGNNCC 5 cut(s) 60, 382, 792, 1333, 1428
NmeAIII GCCGAG 1 cut(s) 1360
NmuCI GTSAC 2 cut(s) 299, 623
NsiI ATGCAT 2 cut(s) 1153, 1414
NspI RCATGY 2 cut(s) 677, 1155
NspV TTCGAA 2 cut(s) 27, 505
PaeI GCATGC 1 cut(s) 677
PaeR7I CTCGAG 1 cut(s) 137
PcsI WCGNNNNNNNCGW 1 cut(s) 59
PctI GAATGC 3 cut(s) 35, 1412, 1455
PdiI GCCGGC 1 cut(s) 1094
PflFI GACNNNGTC 1 cut(s) 224
PkrI GCNGC 5 cut(s) 235, 892, 930, 1029, 1196
PleI GAGTC 4 cut(s) 15, 562, 871, 996
PpsI GAGTC 4 cut(s) 15, 562, 871, 996
Psp124BI GAGCTC 1 cut(s) 138
PspN4I GGNNCC 5 cut(s) 60, 382, 792, 1333, 1428
PspOMI GGGCCC 1 cut(s) 790
PspPI GGNCC 2 cut(s) 790, 791
PsuI RGATCY 2 cut(s) 775, 1426
PsyI GACNNNGTC 1 cut(s) 224
RigI GGCCGGCC 1 cut(s) 1096
RsaI GTAC 1 cut(s) 382
RsaNI GTAC 1 cut(s) 381
RseI CAYNNNNRTG 4 cut(s) 440, 659, 839, 1014
SacI GAGCTC 1 cut(s) 138
SaqAI TTAA 1 cut(s) 798
SatI GCNGC 5 cut(s) 234, 891, 929, 1028, 1195
Sau3AI GATC 5 cut(s) 19, 775, 1081, 1218, 1426
Sau96I GGNCC 2 cut(s) 790, 791
SchI GAGTC 4 cut(s) 16, 563, 872, 997
ScrFI CCNGG 1 cut(s) 72
SduI GDGCHC 2 cut(s) 138, 794
SfaNI GCATC 5 cut(s) 902, 1130, 1138, 1354, 1399
SfcI CTRYAG 2 cut(s) 1036, 1248
Sfr274I CTCGAG 1 cut(s) 137
SfuI TTCGAA 2 cut(s) 27, 505
SlaI CTCGAG 1 cut(s) 137
SmiMI CAYNNNNRTG 4 cut(s) 440, 659, 839, 1014
SmlI CTYRAG 1 cut(s) 137
SmoI CTYRAG 1 cut(s) 137
SphI GCATGC 1 cut(s) 677
Sse9I AATT 6 cut(s) 95, 184, 1049, 1129, 1475, 1492
SsiI CCGC 8 cut(s) 177, 217, 234, 412, 765, 984, 1054, 1394
SspI AATATT 1 cut(s) 1108
SspMI CTAG 2 cut(s) 120, 759
SstI GAGCTC 1 cut(s) 138
StyD4I CCNGG 1 cut(s) 70
StyI CCWWGG 3 cut(s) 119, 840, 1422
TaaI ACNGT 4 cut(s) 58, 265, 541, 1252
TaiI ACGT 4 cut(s) 48, 255, 289, 504
TaqI TCGA 9 cut(s) 27, 42, 104, 138, 160, 505, 557, 769, 870
TasI AATT 6 cut(s) 95, 184, 1049, 1129, 1475, 1492
TauI GCSGC 1 cut(s) 236
Tru1I TTAA 1 cut(s) 798
Tru9I TTAA 1 cut(s) 798
TscAI CASTG 2 cut(s) 736, 1257
TseFI GTSAC 2 cut(s) 299, 623
TseI GCWGC 4 cut(s) 890, 928, 1027, 1194
Tsp45I GTSAC 2 cut(s) 299, 623
TspDTI ATGAA 9 cut(s) 381, 452, 524, 677, 819, 827, 951, 1061, 1475
TspGWI ACGGA 4 cut(s) 184, 188, 963, 1419
TspRI CASTG 2 cut(s) 736, 1257
Tth111I GACNNNGTC 1 cut(s) 224
XapI RAATTY 2 cut(s) 95, 1129
XceI RCATGY 2 cut(s) 677, 1155
XcmI CCANNNNNNNNNTGG 2 cut(s) 391, 913
XhoI CTCGAG 1 cut(s) 137
XmaJI CCTAGG 1 cut(s) 119
XmiI GTMKAC 1 cut(s) 267
XspI CTAG 2 cut(s) 120, 759
ZraI GACGTC 1 cut(s) 253
Zsp2I ATGCAT 2 cut(s) 1153, 1414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.