pycom14g20700

GEM-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0003141
Physical Location & Seq
Forward (+)
21703188 .. 21704200
1013 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 777 bp
ATGAATGAGAAATTTGCAGATCAATTTGTTGCCAGTAGCAGTCCGTACATGATGCACTCATATGATTATGATAGTGATACAACACCAGAAAGGCAAAGCCAAAGCCAAAGGCAGGCATCTTCTCTATCTGAACCTATCAGCTTATGCCATAGTCCTTCTGCACACTCATCTTCCTCCTTCAAAACCACCTCCACCTATGGAGATGATTCGAGGAAAGGCCTCAACAAAAAATTAGGGAAGCAAAGTAGCAGTTTCGCATACAGGATACGCGACCACGTGAAACTTGGGCACAAGCTGTCAGAAACAGTGAAGGGCAAGTTGAGTTTGGGGGCAAGAATCATTCAAGAGGGTGGGAGGGCCAATATTTTTAAACAGATATTCGGTGTCAGTCAAGGAGAAGAGCTTTTGAAGGCCTCCCAATGCTACCTATCTACCACCGCCGGTCCCATTCCTGGCCTCCTTTTCATTTCTACCCAAAAGCTTGCTTTTTGCAGCGAAAGACCCATCACTCTCCCTTCTTCTCCTTCTGCTTCTGCTCCTGCTGCTGCTGGACAACTCCAACCCCTCAGAACGCATTACAAAGTTCAGATACCAATAAGGAAGATCAAAACAGCGAACCAAAGCGAGAATGTCAACAAACCACAACAGAAGTACATTGAAATTGTTACCCAAGATGATTTCGACTTCTGGTTCATGGGATTCTTGCGCTACGAGAAAGCTTTCAGAAATCTTCACAAGGCCCTTTCCATTGCCAGCCACAACCAGTCCCACAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAM

Protein Analysis

259

Amino Acids

28.73

Weight (kDa)

9.55

Isoelectric Point (pI)

63.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 123 - 248 3.9e-11 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 270
AciI CCGC 1 cut(s) 438
AcsI RAATTY 1 cut(s) 11
AcvI CACGTG 1 cut(s) 277
AfaI GTAC 2 cut(s) 47, 653
AfiI CCNNNNNNNGG 2 cut(s) 112, 452
AgsI TTSAA 4 cut(s) 181, 344, 409, 659
AjnI CCWGG 1 cut(s) 451
AluBI AGCT 5 cut(s) 141, 295, 403, 481, 719
AluI AGCT 5 cut(s) 141, 295, 403, 481, 719
AoxI GGCC 5 cut(s) 217, 357, 411, 454, 738
ApeKI GCWGC 3 cut(s) 492, 542, 545
ApoI RAATTY 1 cut(s) 11
Asp700I GAANNNNTTC 1 cut(s) 719
AspLEI GCGC 1 cut(s) 708
AspS9I GGNCC 3 cut(s) 357, 443, 739
AvaII GGWCC 1 cut(s) 443
BaeGI GKGCMC 1 cut(s) 291
BbrPI CACGTG 1 cut(s) 277
BbvI GCAGC 3 cut(s) 504, 529, 532
BccI CCATC 1 cut(s) 512
BciT130I CCWGG 1 cut(s) 453
BciVI GTATCC 1 cut(s) 258
BfaI CTAG 1 cut(s) 775
BfuI GTATCC 1 cut(s) 258
BisI GCNGC 3 cut(s) 493, 543, 546
BlsI GCNGC 3 cut(s) 494, 544, 547
Bme1390I CCNGG 1 cut(s) 453
Bme18I GGWCC 1 cut(s) 443
BmgT120I GGNCC 3 cut(s) 357, 443, 739
BmiI GGNNCC 1 cut(s) 445
BmrFI CCNGG 1 cut(s) 453
BmsI GCATC 2 cut(s) 42, 125
BsaAI YACGTR 1 cut(s) 277
Bsc4I CCNNNNNNNGG 2 cut(s) 112, 452
Bse118I RCCGGY 1 cut(s) 440
Bse1I ACTGG 2 cut(s) 33, 763
Bse3DI GCAATG 1 cut(s) 747
BseBI CCWGG 1 cut(s) 453
BseLI CCNNNNNNNGG 2 cut(s) 112, 452
BseMI GCAATG 1 cut(s) 747
BseMII CTCAG 1 cut(s) 580
BseNI ACTGG 2 cut(s) 33, 763
BseSI GKGCMC 1 cut(s) 291
BseXI GCAGC 3 cut(s) 504, 529, 532
BsgI GTGCAG 1 cut(s) 144
Bsh1236I CGCG 1 cut(s) 270
BshFI GGCC 5 cut(s) 219, 359, 413, 456, 740
BsiSI CCGG 1 cut(s) 441
BslFI GGGAC 2 cut(s) 429, 751
BslI CCNNNNNNNGG 2 cut(s) 112, 452
BsmFI GGGAC 2 cut(s) 429, 751
BsnI GGCC 5 cut(s) 219, 359, 413, 456, 740
Bsp1286I GDGCHC 1 cut(s) 291
Bsp143I GATC 2 cut(s) 19, 603
BspACI CCGC 1 cut(s) 438
BspANI GGCC 5 cut(s) 219, 359, 413, 456, 740
BspCNI CTCAG 1 cut(s) 579
BspFNI CGCG 1 cut(s) 270
BspLI GGNNCC 1 cut(s) 445
BspQI GCTCTTC 1 cut(s) 393
BsrDI GCAATG 1 cut(s) 747
BsrFI RCCGGY 1 cut(s) 440
BsrI ACTGG 2 cut(s) 33, 763
BssAI RCCGGY 1 cut(s) 440
BssMI GATC 2 cut(s) 19, 603
Bst2UI CCWGG 1 cut(s) 453
Bst4CI ACNGT 1 cut(s) 307
Bst6I CTCTTC 1 cut(s) 393
BstBAI YACGTR 1 cut(s) 277
BstC8I GCNNGC 3 cut(s) 114, 483, 754
BstDEI CTNAG 1 cut(s) 566
BstFNI CGCG 1 cut(s) 270
BstHHI GCGC 1 cut(s) 708
BstKTI GATC 2 cut(s) 22, 606
BstMBI GATC 2 cut(s) 19, 603
BstMWI GCNNNNNNNGC 1 cut(s) 542
BstNI CCWGG 1 cut(s) 453
BstSCI CCNGG 1 cut(s) 451
BstSLI GKGCMC 1 cut(s) 291
BstUI CGCG 1 cut(s) 270
BstV1I GCAGC 3 cut(s) 504, 529, 532
BsuI GTATCC 1 cut(s) 258
BsuRI GGCC 5 cut(s) 219, 359, 413, 456, 740
BtsIMutI CAGTG 1 cut(s) 312
Cac8I GCNNGC 3 cut(s) 114, 483, 754
CfoI GCGC 1 cut(s) 708
Cfr10I RCCGGY 1 cut(s) 440
Cfr13I GGNCC 3 cut(s) 357, 443, 739
Csp6I GTAC 2 cut(s) 46, 652
CviAII CATG 2 cut(s) 49, 694
CviQI GTAC 2 cut(s) 46, 652
DdeI CTNAG 1 cut(s) 566
DpnI GATC 2 cut(s) 21, 605
DpnII GATC 2 cut(s) 19, 603
DraI TTTAAA 1 cut(s) 370
Eam1104I CTCTTC 1 cut(s) 393
EarI CTCTTC 1 cut(s) 393
Eco147I AGGCCT 2 cut(s) 219, 413
Eco47I GGWCC 1 cut(s) 443
Eco72I CACGTG 1 cut(s) 277
EcoO109I RGGNCCY 1 cut(s) 739
EcoRII CCWGG 1 cut(s) 451
FaeI CATG 2 cut(s) 52, 697
FaiI YATR 9 cut(s) 50, 61, 63, 69, 145, 150, 198, 259, 695
FaqI GGGAC 2 cut(s) 429, 751
FatI CATG 2 cut(s) 48, 693
FauNDI CATATG 1 cut(s) 61
Fnu4HI GCNGC 3 cut(s) 493, 543, 546
Fsp4HI GCNGC 3 cut(s) 493, 543, 546
FspBI CTAG 1 cut(s) 775
GlaI GCGC 1 cut(s) 707
GluI GCNGC 3 cut(s) 493, 543, 546
HaeIII GGCC 5 cut(s) 219, 359, 413, 456, 740
HapII CCGG 1 cut(s) 441
HhaI GCGC 1 cut(s) 708
Hin1II CATG 2 cut(s) 52, 697
Hin6I GCGC 1 cut(s) 706
HinP1I GCGC 1 cut(s) 706
HincII GTYRAC 1 cut(s) 634
HindII GTYRAC 1 cut(s) 634
HindIII AAGCTT 2 cut(s) 479, 717
HinfI GANTC 3 cut(s) 206, 336, 699
HpaII CCGG 1 cut(s) 441
Hpy166II GTNNAC 1 cut(s) 634
Hpy188I TCNGA 5 cut(s) 130, 301, 569, 588, 725
Hpy188III TCNNGA 1 cut(s) 344
Hpy8I GTNNAC 1 cut(s) 634
HpyAV CCTTC 6 cut(s) 165, 187, 304, 403, 525, 534
HpyCH4III ACNGT 1 cut(s) 307
HpyCH4IV ACGT 1 cut(s) 276
HpyCH4V TGCA 4 cut(s) 17, 55, 161, 492
HpyF10VI GCNNNNNNNGC 1 cut(s) 542
HpyF3I CTNAG 1 cut(s) 566
HpySE526I ACGT 1 cut(s) 276
Hsp92II CATG 2 cut(s) 52, 697
HspAI GCGC 1 cut(s) 706
Kzo9I GATC 2 cut(s) 19, 603
LguI GCTCTTC 1 cut(s) 393
LmnI GCTCC 1 cut(s) 541
Lsp1109I GCAGC 3 cut(s) 504, 529, 532
LweI GCATC 2 cut(s) 42, 125
MaeI CTAG 1 cut(s) 775
MaeII ACGT 1 cut(s) 276
MaeIII GTNAC 1 cut(s) 664
MalI GATC 2 cut(s) 21, 605
MboI GATC 2 cut(s) 19, 603
MboII GAAGA 6 cut(s) 111, 162, 410, 510, 613, 722
MhlI GDGCHC 1 cut(s) 291
MluCI AATT 4 cut(s) 11, 23, 230, 660
MmeI TCCRAC 1 cut(s) 583
MnlI CCTC 9 cut(s) 184, 199, 204, 230, 340, 348, 424, 467, 575
MroXI GAANNNNTTC 1 cut(s) 719
MseI TTAA 1 cut(s) 369
MslI CAYNNNNRTG 1 cut(s) 60
MspI CCGG 1 cut(s) 441
MspR9I CCNGG 1 cut(s) 453
MvaI CCWGG 1 cut(s) 453
MvnI CGCG 1 cut(s) 270
MwoI GCNNNNNNNGC 1 cut(s) 542
NdeI CATATG 1 cut(s) 61
NdeII GATC 2 cut(s) 19, 603
NlaIII CATG 2 cut(s) 52, 697
NlaIV GGNNCC 1 cut(s) 445
PceI AGGCCT 2 cut(s) 219, 413
PciSI GCTCTTC 1 cut(s) 393
PdmI GAANNNNTTC 1 cut(s) 719
PfeI GAWTC 3 cut(s) 206, 336, 699
PkrI GCNGC 3 cut(s) 494, 544, 547
PmaCI CACGTG 1 cut(s) 277
PmlI CACGTG 1 cut(s) 277
Ppu21I YACGTR 1 cut(s) 277
Psp6I CCWGG 1 cut(s) 451
PspCI CACGTG 1 cut(s) 277
PspGI CCWGG 1 cut(s) 451
PspN4I GGNNCC 1 cut(s) 445
PspPI GGNCC 3 cut(s) 357, 443, 739
RsaI GTAC 2 cut(s) 47, 653
RsaNI GTAC 2 cut(s) 46, 652
RseI CAYNNNNRTG 1 cut(s) 60
SapI GCTCTTC 1 cut(s) 393
SaqAI TTAA 1 cut(s) 369
SatI GCNGC 3 cut(s) 493, 543, 546
Sau3AI GATC 2 cut(s) 19, 603
Sau96I GGNCC 3 cut(s) 357, 443, 739
ScrFI CCNGG 1 cut(s) 453
SduI GDGCHC 1 cut(s) 291
SfaNI GCATC 2 cut(s) 42, 125
SinI GGWCC 1 cut(s) 443
SmiMI CAYNNNNRTG 1 cut(s) 60
Sse9I AATT 4 cut(s) 11, 23, 230, 660
SseBI AGGCCT 2 cut(s) 219, 413
SsiI CCGC 1 cut(s) 438
SspI AATATT 1 cut(s) 364
SspMI CTAG 1 cut(s) 775
StuI AGGCCT 2 cut(s) 219, 413
StyD4I CCNGG 1 cut(s) 451
TaaI ACNGT 1 cut(s) 307
TaiI ACGT 1 cut(s) 279
TaqI TCGA 2 cut(s) 209, 681
TasI AATT 4 cut(s) 11, 23, 230, 660
TatI WGTACW 1 cut(s) 651
TfiI GAWTC 3 cut(s) 206, 336, 699
Tru1I TTAA 1 cut(s) 369
Tru9I TTAA 1 cut(s) 369
TscAI CASTG 1 cut(s) 312
TseI GCWGC 3 cut(s) 492, 542, 545
TspDTI ATGAA 3 cut(s) 17, 454, 682
TspGWI ACGGA 1 cut(s) 33
TspRI CASTG 1 cut(s) 312
VpaK11BI GGWCC 1 cut(s) 443
XapI RAATTY 1 cut(s) 11
XcmI CCANNNNNNNNNTGG 1 cut(s) 281
XmnI GAANNNNTTC 1 cut(s) 719
XspI CTAG 1 cut(s) 775
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.