pycom15g01840

GTP-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0003824
Physical Location & Seq
Forward (+)
1054714 .. 1059517
4804 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1815 bp
ATGCGGAGGTCTTCAGCAACGACACGTCGTATATACAATTTGCGGCCTGTAGGAAAGAACGCGCGAGAGAACACCCTGCAAGCCAACGATAGCCAGAGACTCTTCGGAAGTGGAAAGCTGAAAGCTCTCTCTCCGACCAAATTTCCGATCATGAGCGCATGCTTCTGCTGCTCTCTGATTCGTTCCTACCCTCTCAGCCACGATAATCTCATTTCCCGCCCTCTCGCGAAACTCAGTCGTCCGATTTTCTCTCTCGCGCTCAGAAAACAATATTCCGATGCTACTGCTCCTAGATTCGTCGTCGTAAGAGCCGTCCAGCCCGGCCTCGGCGTTGTGGAACCGGGAGACGTGTCGGTGGGGGACCCGCCGTCGATTGATGTTGTAGAGGCCGGAAATGGAAAGGTTCTCGGAGTCGTTGGTGGAGTGGCGGACGCCAAATTGGAGGATGATAAACCCCTAGCTCCGGCGACTAGGGTTAGGAAGAAGAAGGAGGAGGAGGGCGGCGATGATAATCGGTTCAAGCTTAGGAACGGAAGGGAGGTTTTCGAAGAAAAAGCTTACATTGTGGGTGTTGAGCACAAACGCGGTACAGCGGATTCGTTTGGTGTGGAGGAATCACTCAAGGAATTGACCCAGCTAGCTGATACTGCTGGACTTATGGTTGTTGGTTCCACGTATCAAAAAAGTAGACTCGTTTCTCCAAACTCGAGGACGTATATTGGGTCTGGCAAGGTTGCAGAAATCAAGAGTGCAATTAACGCATTGGGTGTGGAGACTGTGATATTCGATGATGAGCTATCAGCAGGGCAATTGCGCAATTTGGAAAAGGCGTTTGGTGGAGAGGTTAGAGTTTGTGACCGCACTGCCCTCATTCTGGATATTTTTAACCAGCGGGCTGCAACACATGAAGCAGCTTTACAGGTTGCATTGGCACAAATGGAATACCAGTTACCTCGGCTAACAAAAATGTGGACCCATCTTGAGCGTCAAGCCGGTGGACAGGTTAAGGGGATGGGTGAGAAACAAATTGAAGTGGATAAACGTATCCTACGTACTCAAATTGGTGTTCTCAAGAAGGAGCTAGAATCTGTTCGAAAGCATAGAAAGCAGTACCGGAATCAGCGGCTTTCTGTACCTGTCCCTGTAGTATCTTTGGTTGGGTACACTAATGCTGGAAAGAGTACTCTTTTAAATCAATTGACCGGAGCCAATGTCCTTGCCGAGGATCGATTATTTGCAACCCTAGATCCAACCACAAGAAGGGTTCAGACGAAGAATGGGAAAGAGTTTCTTCTCACAGATACTGTTGGTTTCATTCAGAAGTTACCAACTACAGTGGTTGCTGCCTTCAGAGCAACACTGGAGGAGATATCAGAGTCATCATTGTTGGTGCATGTGGTGGATATCAGTCATCCACTGGCTGAGCAACAGATCGAAGCTGTGGAGAAAGTTTTGTCAGAACTAGACGTGGCCGCAATTCCAACGTTGATGGTGTGGAACAAGGTTGATAAGGTTAGCAATCCTGAAAGCATAAAGTTGGAAGCAGAAAAGAGAGATGATGTGATTTGCGTATCTGCTCTGAGTGGTGAGGGCATAAATGAATTCTGCAGTGCAGTTCAGGAAAAATTGAAGGACTCCATGGTATGGGTGGAAGCCTTGATCCCGTTCGAGAAAGGGGAGCTCCTCAGTACCATACATAAGGTTGGAATGGTGGAGAGAACGGAATATACGGACAAAGGAACGCTGATAAAGGCACACGTACCACTGCGGTATGCAAGGCTACTAACACCAATGAGGCAACTATGCATATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GTP-bdg_M GTP-bdg_N MMR_HSR1

Protein Analysis

605

Amino Acids

66.56

Weight (kDa)

9.1

Isoelectric Point (pI)

33.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP-bdg_N PF13167 204 - 295 3.2e-28 GTP-binding GTPase N-terminal
GTP-bdg_M PF16360 298 - 375 5.5e-27 GTP-binding GTPase Middle Region
MMR_HSR1 PF01926 383 - 501 5e-22 50S ribosome-binding GTPase
FeoB_N PF02421 383 - 537 3.5e-06 Ferrous iron transport protein B
HflX_C PF19275 511 - 582 1.2e-08 HflX C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 815
AccB7I CCANNNNNTGG 1 cut(s) 1644
AccI GTMKAC 1 cut(s) 688
AccII CGCG 5 cut(s) 62, 64, 227, 257, 585
AclI AACGTT 1 cut(s) 1484
AclWI GGATC 3 cut(s) 1233, 1241, 1654
AcoI YGGCCR 1 cut(s) 1470
AcsI RAATTY 2 cut(s) 140, 1601
AcuI CTGAAG 1 cut(s) 1333
AcyI GRCGYC 1 cut(s) 432
AfaI GTAC 8 cut(s) 589, 1054, 1112, 1134, 1163, 1183, 1690, 1761
AfiI CCNNNNNNNGG 6 cut(s) 326, 463, 874, 1222, 1260, 1644
AflIII ACRYGT 3 cut(s) 23, 348, 1756
AgsI TTSAA 3 cut(s) 520, 1031, 1630
AhdI GACNNNNNGTC 1 cut(s) 367
AjiI CACGTC 3 cut(s) 26, 349, 1468
AjuI GAANNNNNNNTTGG 2 cut(s) 816, 848
Alw21I GWGCWC 2 cut(s) 579, 1683
Alw26I GTCTC 3 cut(s) 91, 339, 767
AlwI GGATC 3 cut(s) 1233, 1241, 1654
AlwNI CAGNNNCTG 1 cut(s) 1304
Ama87I CYCGRG 1 cut(s) 706
AoxI GGCC 4 cut(s) 44, 322, 387, 1470
ApeKI GCWGC 4 cut(s) 168, 896, 911, 1343
ApoI RAATTY 2 cut(s) 140, 1601
Asp700I GAANNNNTTC 1 cut(s) 1089
AspLEI GCGC 4 cut(s) 64, 158, 259, 816
AspS9I GGNCC 2 cut(s) 361, 972
AsuC2I CCSGG 2 cut(s) 321, 342
AsuHPI GGTGA 2 cut(s) 1028, 1598
AsuII TTCGAA 2 cut(s) 546, 1093
AsuNHI GCTAGC 1 cut(s) 637
AvaI CYCGRG 1 cut(s) 706
AvaII GGWCC 2 cut(s) 361, 972
BanII GRGCYC 1 cut(s) 1683
BarI GAAGNNNNNNTAC 2 cut(s) 900, 932
BbsI GAAGAC 1 cut(s) 3
Bbv12I GWGCWC 2 cut(s) 579, 1683
BbvI GCAGC 4 cut(s) 155, 883, 923, 1330
BccI CCATC 3 cut(s) 984, 1006, 1483
BceAI ACGGC 2 cut(s) 296, 352
BcgI CGANNNNNNTGC 2 cut(s) 266, 300
BciVI GTATCC 1 cut(s) 1055
BcnI CCSGG 2 cut(s) 321, 342
BcoDI GTCTC 3 cut(s) 91, 339, 767
BfaI CTAG 7 cut(s) 291, 458, 471, 638, 1082, 1244, 1463
BfmI CTRYAG 4 cut(s) 48, 1143, 1332, 1606
BfuI GTATCC 1 cut(s) 1055
BisI GCNGC 8 cut(s) 44, 169, 502, 897, 912, 1124, 1344, 1473
BlpI GCTNAGC 1 cut(s) 1422
BlsI GCNGC 8 cut(s) 45, 170, 503, 898, 913, 1125, 1345, 1474
BmcAI AGTACT 1 cut(s) 1183
Bme1390I CCNGG 2 cut(s) 321, 342
Bme18I GGWCC 2 cut(s) 361, 972
BmeRI GACNNNNNGTC 1 cut(s) 367
BmeT110I CYCGRG 1 cut(s) 706
BmgBI CACGTC 3 cut(s) 26, 349, 1468
BmgT120I GGNCC 2 cut(s) 361, 972
BmiI GGNNCC 6 cut(s) 339, 362, 363, 670, 974, 1207
BmrFI CCNGG 2 cut(s) 321, 342
BmsI GCATC 1 cut(s) 268
BmtI GCTAGC 1 cut(s) 641
BpiI GAAGAC 1 cut(s) 3
BpmI CTGGAG 1 cut(s) 1382
Bpu10I CCTNAGC 1 cut(s) 524
Bpu1102I GCTNAGC 1 cut(s) 1422
Bpu14I TTCGAA 2 cut(s) 546, 1093
BpuEI CTTGAG 3 cut(s) 605, 1001, 1055
BpuMI CCSGG 2 cut(s) 321, 342
Bsa29I ATCGAT 1 cut(s) 1228
BsaAI YACGTR 3 cut(s) 675, 1052, 1759
BsaBI GATNNNNATC 1 cut(s) 510
BsaHI GRCGYC 1 cut(s) 432
BsaJI CCNNGG 4 cut(s) 325, 953, 1221, 1638
BsaWI WCCGGW 2 cut(s) 1113, 1202
BsaXI ACNNNNNCTCC 2 cut(s) 1197, 1227
Bsc4I CCNNNNNNNGG 6 cut(s) 326, 463, 874, 1222, 1260, 1644
Bse118I RCCGGY 1 cut(s) 992
Bse1I ACTGG 3 cut(s) 946, 1365, 1422
Bse8I GATNNNNATC 1 cut(s) 510
BseCI ATCGAT 1 cut(s) 1228
BseDI CCNNGG 4 cut(s) 325, 953, 1221, 1638
BseGI GGATG 3 cut(s) 451, 1017, 1411
BseJI GATNNNNATC 1 cut(s) 510
BseLI CCNNNNNNNGG 6 cut(s) 326, 463, 874, 1222, 1260, 1644
BseMII CTCAG 6 cut(s) 208, 247, 274, 1413, 1571, 1699
BseNI ACTGG 3 cut(s) 946, 1365, 1422
BseRI GAGGAG 4 cut(s) 506, 509, 1379, 1673
BseXI GCAGC 4 cut(s) 155, 883, 923, 1330
BseYI CCCAGC 1 cut(s) 633
BsgI GTGCAG 1 cut(s) 1632
Bsh1236I CGCG 5 cut(s) 62, 64, 227, 257, 585
BshFI GGCC 4 cut(s) 46, 324, 389, 1472
BshVI ATCGAT 1 cut(s) 1228
BsiHKAI GWGCWC 2 cut(s) 579, 1683
BsiHKCI CYCGRG 1 cut(s) 706
BsiSI CCGG 7 cut(s) 321, 341, 390, 464, 993, 1114, 1203
BslFI GGGAC 2 cut(s) 374, 1124
BslI CCNNNNNNNGG 6 cut(s) 326, 463, 874, 1222, 1260, 1644
BsmAI GTCTC 3 cut(s) 91, 339, 767
BsmBI CGTCTC 1 cut(s) 339
BsmFI GGGAC 2 cut(s) 374, 1124
BsnI GGCC 4 cut(s) 46, 324, 389, 1472
BsoBI CYCGRG 1 cut(s) 706
Bsp119I TTCGAA 2 cut(s) 546, 1093
Bsp1286I GDGCHC 2 cut(s) 579, 1683
Bsp143I GATC 5 cut(s) 147, 1225, 1246, 1431, 1659
Bsp1720I GCTNAGC 1 cut(s) 1422
Bsp19I CCATGG 1 cut(s) 1638
Bsp68I TCGCGA 1 cut(s) 227
BspANI GGCC 4 cut(s) 46, 324, 389, 1472
BspCNI CTCAG 6 cut(s) 207, 246, 273, 1414, 1572, 1698
BspDI ATCGAT 1 cut(s) 1228
BspFNI CGCG 5 cut(s) 62, 64, 227, 257, 585
BspHI TCATGA 2 cut(s) 150, 1811
BspLI GGNNCC 6 cut(s) 339, 362, 363, 670, 974, 1207
BspMAI CTGCAG 1 cut(s) 1610
BspOI GCTAGC 1 cut(s) 641
BspPI GGATC 3 cut(s) 1233, 1241, 1654
BspT104I TTCGAA 2 cut(s) 546, 1093
BsrFI RCCGGY 1 cut(s) 992
BsrI ACTGG 3 cut(s) 946, 1365, 1422
BssAI RCCGGY 1 cut(s) 992
BssECI CCNNGG 4 cut(s) 325, 953, 1221, 1638
BssMI GATC 5 cut(s) 147, 1225, 1246, 1431, 1659
BssNI GRCGYC 1 cut(s) 432
BssT1I CCWWGG 1 cut(s) 1638
Bst4CI ACNGT 3 cut(s) 778, 1306, 1336
Bst6I CTCTTC 1 cut(s) 107
BstACI GRCGYC 1 cut(s) 432
BstBAI YACGTR 3 cut(s) 675, 1052, 1759
BstBI TTCGAA 2 cut(s) 546, 1093
BstC8I GCNNGC 4 cut(s) 81, 160, 639, 894
BstDEI CTNAG 7 cut(s) 194, 233, 260, 524, 1422, 1580, 1685
BstDSI CCRYGG 1 cut(s) 1638
BstENI CCTNNNNNAGG 1 cut(s) 1220
BstF5I GGATG 3 cut(s) 451, 1017, 1411
BstFNI CGCG 5 cut(s) 62, 64, 227, 257, 585
BstHHI GCGC 4 cut(s) 64, 158, 259, 816
BstKTI GATC 5 cut(s) 150, 1228, 1249, 1434, 1662
BstMAI GTCTC 3 cut(s) 91, 339, 767
BstMBI GATC 5 cut(s) 147, 1225, 1246, 1431, 1659
BstMWI GCNNNNNNNGC 5 cut(s) 168, 647, 758, 1105, 1352
BstNSI RCATGY 2 cut(s) 162, 1397
BstSCI CCNGG 2 cut(s) 319, 340
BstSFI CTRYAG 4 cut(s) 48, 1143, 1332, 1606
BstSNI TACGTA 1 cut(s) 1052
BstUI CGCG 5 cut(s) 62, 64, 227, 257, 585
BstV1I GCAGC 4 cut(s) 155, 883, 923, 1330
BstV2I GAAGAC 1 cut(s) 3
BstX2I RGATCY 1 cut(s) 1246
BstYI RGATCY 1 cut(s) 1246
Bsu15I ATCGAT 1 cut(s) 1228
BsuI GTATCC 1 cut(s) 1055
BsuRI GGCC 4 cut(s) 46, 324, 389, 1472
BsuTUI ATCGAT 1 cut(s) 1228
BtgI CCRYGG 1 cut(s) 1638
BtgZI GCGATG 1 cut(s) 519
BtrI CACGTC 3 cut(s) 26, 349, 1468
BtsCI GGATG 3 cut(s) 451, 1017, 1411
BtsI GCAGTG 3 cut(s) 861, 1615, 1763
BtsIMutI CAGTG 6 cut(s) 861, 1341, 1358, 1415, 1615, 1763
BtuMI TCGCGA 1 cut(s) 227
Cac8I GCNNGC 4 cut(s) 81, 160, 639, 894
CaiI CAGNNNCTG 1 cut(s) 1304
CciI TCATGA 2 cut(s) 150, 1811
CfoI GCGC 4 cut(s) 64, 158, 259, 816
Cfr10I RCCGGY 1 cut(s) 992
Cfr13I GGNCC 2 cut(s) 361, 972
ClaI ATCGAT 1 cut(s) 1228
CseI GACGC 2 cut(s) 440, 974
Csp6I GTAC 8 cut(s) 588, 1053, 1111, 1133, 1162, 1182, 1689, 1760
CspCI CAANNNNNGTGG 2 cut(s) 1317, 1352
CviAII CATG 6 cut(s) 151, 159, 905, 1394, 1639, 1812
CviQI GTAC 8 cut(s) 588, 1053, 1111, 1133, 1162, 1182, 1689, 1760
DdeI CTNAG 7 cut(s) 194, 233, 260, 524, 1422, 1580, 1685
DpnI GATC 5 cut(s) 149, 1227, 1248, 1433, 1661
DpnII GATC 5 cut(s) 147, 1225, 1246, 1431, 1659
DraI TTTAAA 1 cut(s) 1191
DriI GACNNNNNGTC 1 cut(s) 367
EaeI YGGCCR 1 cut(s) 1470
Eam1104I CTCTTC 1 cut(s) 107
Eam1105I GACNNNNNGTC 1 cut(s) 367
EarI CTCTTC 1 cut(s) 107
EciI GGCGGA 1 cut(s) 443
Ecl136II GAGCTC 1 cut(s) 1681
Eco105I TACGTA 1 cut(s) 1052
Eco130I CCWWGG 1 cut(s) 1638
Eco24I GRGCYC 1 cut(s) 1683
Eco32I GATATC 2 cut(s) 1371, 1405
Eco47I GGWCC 2 cut(s) 361, 972
Eco53kI GAGCTC 1 cut(s) 1681
Eco57I CTGAAG 1 cut(s) 1333
Eco88I CYCGRG 1 cut(s) 706
EcoICRI GAGCTC 1 cut(s) 1681
EcoNI CCTNNNNNAGG 1 cut(s) 1220
EcoO109I RGGNCCY 1 cut(s) 361
EcoRI GAATTC 1 cut(s) 1601
EcoRV GATATC 2 cut(s) 1371, 1405
EcoT14I CCWWGG 1 cut(s) 1638
EcoT22I ATGCAT 1 cut(s) 1808
EcoT38I GRGCYC 1 cut(s) 1683
ErhI CCWWGG 1 cut(s) 1638
Esp3I CGTCTC 1 cut(s) 339
FaeI CATG 6 cut(s) 154, 162, 908, 1397, 1642, 1815
FalI AAGNNNNNCTT 2 cut(s) 1275, 1307
FaqI GGGAC 2 cut(s) 374, 1124
FatI CATG 6 cut(s) 150, 158, 904, 1393, 1638, 1811
FauI CCCGC 3 cut(s) 224, 372, 885
FblI GTMKAC 1 cut(s) 688
Fnu4HI GCNGC 8 cut(s) 44, 169, 502, 897, 912, 1124, 1344, 1473
FokI GGATG 3 cut(s) 458, 1024, 1398
FriOI GRGCYC 1 cut(s) 1683
Fsp4HI GCNGC 8 cut(s) 44, 169, 502, 897, 912, 1124, 1344, 1473
FspBI CTAG 7 cut(s) 291, 458, 471, 638, 1082, 1244, 1463
FspI TGCGCA 1 cut(s) 815
GlaI GCGC 4 cut(s) 63, 157, 258, 815
GluI GCNGC 8 cut(s) 44, 169, 502, 897, 912, 1124, 1344, 1473
GsaI CCCAGC 1 cut(s) 637
GsuI CTGGAG 1 cut(s) 1382
HaeIII GGCC 4 cut(s) 46, 324, 389, 1472
HapII CCGG 7 cut(s) 321, 341, 390, 464, 993, 1114, 1203
HgaI GACGC 2 cut(s) 440, 974
HhaI GCGC 4 cut(s) 64, 158, 259, 816
Hin1I GRCGYC 1 cut(s) 432
Hin1II CATG 6 cut(s) 154, 162, 908, 1397, 1642, 1815
Hin6I GCGC 4 cut(s) 62, 156, 257, 814
HinP1I GCGC 4 cut(s) 62, 156, 257, 814
HindIII AAGCTT 2 cut(s) 521, 555
HpaII CCGG 7 cut(s) 321, 341, 390, 464, 993, 1114, 1203
HphI GGTGA 2 cut(s) 1028, 1598
Hpy166II GTNNAC 4 cut(s) 689, 972, 998, 1164
Hpy8I GTNNAC 4 cut(s) 689, 972, 998, 1164
Hpy99I CGWCG 4 cut(s) 30, 302, 305, 373
HpyAV CCTTC 6 cut(s) 481, 528, 1069, 1254, 1357, 1624
HpyCH4III ACNGT 3 cut(s) 778, 1306, 1336
HpyCH4IV ACGT 9 cut(s) 25, 348, 674, 713, 1042, 1051, 1467, 1484, 1758
HpyF10VI GCNNNNNNNGC 5 cut(s) 168, 647, 758, 1105, 1352
HpyF3I CTNAG 7 cut(s) 194, 233, 260, 524, 1422, 1580, 1685
HpySE526I ACGT 9 cut(s) 25, 348, 674, 713, 1042, 1051, 1467, 1484, 1758
Hsp92I GRCGYC 1 cut(s) 432
Hsp92II CATG 6 cut(s) 154, 162, 908, 1397, 1642, 1815
HspAI GCGC 4 cut(s) 62, 156, 257, 814
KflI GGGWCCC 1 cut(s) 361
Kzo9I GATC 5 cut(s) 147, 1225, 1246, 1431, 1659
LmnI GCTCC 6 cut(s) 292, 466, 1078, 1205, 1678, 1686
Lsp1109I GCAGC 4 cut(s) 155, 883, 923, 1330
LweI GCATC 1 cut(s) 268
MaeI CTAG 7 cut(s) 291, 458, 471, 638, 1082, 1244, 1463
MaeII ACGT 9 cut(s) 25, 348, 674, 713, 1042, 1051, 1467, 1484, 1758
MaeIII GTNAC 3 cut(s) 854, 948, 1323
MalI GATC 5 cut(s) 149, 1227, 1248, 1433, 1661
MboI GATC 5 cut(s) 147, 1225, 1246, 1431, 1659
MboII GAAGA 7 cut(s) 3, 94, 493, 496, 560, 1283, 1285
MfeI CAATTG 2 cut(s) 809, 1196
MflI RGATCY 1 cut(s) 1246
MhlI GDGCHC 2 cut(s) 579, 1683
MlyI GAGTC 5 cut(s) 93, 420, 684, 1385, 1628
MmeI TCCRAC 5 cut(s) 158, 1274, 1505, 1518, 1684
Mph1103I ATGCAT 1 cut(s) 1808
MroXI GAANNNNTTC 1 cut(s) 1089
MseI TTAA 4 cut(s) 756, 885, 1005, 1190
MspA1I CMGCKG 3 cut(s) 593, 892, 1123
MspI CCGG 7 cut(s) 321, 341, 390, 464, 993, 1114, 1203
MspR9I CCNGG 2 cut(s) 321, 342
MunI CAATTG 2 cut(s) 809, 1196
MvnI CGCG 5 cut(s) 62, 64, 227, 257, 585
MwoI GCNNNNNNNGC 5 cut(s) 168, 647, 758, 1105, 1352
NciI CCSGG 2 cut(s) 321, 342
NcoI CCATGG 1 cut(s) 1638
NdeII GATC 5 cut(s) 147, 1225, 1246, 1431, 1659
NheI GCTAGC 1 cut(s) 637
NlaIII CATG 6 cut(s) 154, 162, 908, 1397, 1642, 1815
NlaIV GGNNCC 6 cut(s) 339, 362, 363, 670, 974, 1207
NmeAIII GCCGAG 3 cut(s) 306, 934, 1246
NmuCI GTSAC 1 cut(s) 854
NruI TCGCGA 1 cut(s) 227
NsbI TGCGCA 1 cut(s) 815
NsiI ATGCAT 1 cut(s) 1808
NspI RCATGY 2 cut(s) 162, 1397
NspV TTCGAA 2 cut(s) 546, 1093
PaeI GCATGC 1 cut(s) 162
PaeR7I CTCGAG 1 cut(s) 706
PagI TCATGA 2 cut(s) 150, 1811
PcsI WCGNNNNNNNCGW 2 cut(s) 309, 1048
PdmI GAANNNNTTC 1 cut(s) 1089
PfeI GAWTC 6 cut(s) 178, 294, 596, 614, 1085, 1117
PflMI CCANNNNNTGG 1 cut(s) 1644
PkrI GCNGC 8 cut(s) 45, 170, 503, 898, 913, 1125, 1345, 1474
PleI GAGTC 5 cut(s) 93, 419, 684, 1384, 1628
PpsI GAGTC 5 cut(s) 93, 419, 684, 1384, 1628
Ppu21I YACGTR 3 cut(s) 675, 1052, 1759
PpuMI RGGWCCY 1 cut(s) 361
Psp124BI GAGCTC 1 cut(s) 1683
Psp1406I AACGTT 1 cut(s) 1484
Psp5II RGGWCCY 1 cut(s) 361
PspFI CCCAGC 1 cut(s) 633
PspN4I GGNNCC 6 cut(s) 339, 362, 363, 670, 974, 1207
PspPI GGNCC 2 cut(s) 361, 972
PspPPI RGGWCCY 1 cut(s) 361
PspXI VCTCGAGB 1 cut(s) 706
PsrI GAACNNNNNNTAC 2 cut(s) 1711, 1743
PstI CTGCAG 1 cut(s) 1610
PstNI CAGNNNCTG 1 cut(s) 1304
PsuI RGATCY 1 cut(s) 1246
RruI TCGCGA 1 cut(s) 227
RsaI GTAC 8 cut(s) 589, 1054, 1112, 1134, 1163, 1183, 1690, 1761
RsaNI GTAC 8 cut(s) 588, 1053, 1111, 1133, 1162, 1182, 1689, 1760
SacI GAGCTC 1 cut(s) 1683
SaqAI TTAA 4 cut(s) 756, 885, 1005, 1190
SatI GCNGC 8 cut(s) 44, 169, 502, 897, 912, 1124, 1344, 1473
Sau3AI GATC 5 cut(s) 147, 1225, 1246, 1431, 1659
Sau96I GGNCC 2 cut(s) 361, 972
ScaI AGTACT 1 cut(s) 1183
SchI GAGTC 5 cut(s) 93, 420, 684, 1385, 1628
ScrFI CCNGG 2 cut(s) 321, 342
SduI GDGCHC 2 cut(s) 579, 1683
SfaNI GCATC 1 cut(s) 268
SfcI CTRYAG 4 cut(s) 48, 1143, 1332, 1606
Sfr274I CTCGAG 1 cut(s) 706
SfuI TTCGAA 2 cut(s) 546, 1093
SinI GGWCC 2 cut(s) 361, 972
SlaI CTCGAG 1 cut(s) 706
SmlI CTYRAG 4 cut(s) 620, 706, 980, 1070
SmoI CTYRAG 4 cut(s) 620, 706, 980, 1070
SnaBI TACGTA 1 cut(s) 1052
SphI GCATGC 1 cut(s) 162
SspI AATATT 1 cut(s) 272
SspMI CTAG 7 cut(s) 291, 458, 471, 638, 1082, 1244, 1463
SstI GAGCTC 1 cut(s) 1683
StyD4I CCNGG 2 cut(s) 319, 340
StyI CCWWGG 1 cut(s) 1638
TaaI ACNGT 3 cut(s) 778, 1306, 1336
TaiI ACGT 9 cut(s) 28, 351, 677, 716, 1045, 1054, 1470, 1487, 1761
TaqI TCGA 8 cut(s) 371, 546, 707, 786, 1093, 1228, 1434, 1668
TatI WGTACW 1 cut(s) 1181
TauI GCSGC 4 cut(s) 46, 504, 1126, 1475
TfiI GAWTC 6 cut(s) 178, 294, 596, 614, 1085, 1117
Tru1I TTAA 4 cut(s) 756, 885, 1005, 1190
Tru9I TTAA 4 cut(s) 756, 885, 1005, 1190
TscAI CASTG 6 cut(s) 868, 1341, 1365, 1422, 1615, 1770
TseFI GTSAC 1 cut(s) 854
TseI GCWGC 4 cut(s) 168, 896, 911, 1343
Tsp45I GTSAC 1 cut(s) 854
TspDTI ATGAA 3 cut(s) 921, 1303, 1614
TspGWI ACGGA 3 cut(s) 546, 1736, 1745
TspRI CASTG 6 cut(s) 868, 1341, 1365, 1422, 1615, 1770
Van91I CCANNNNNTGG 1 cut(s) 1644
VpaK11BI GGWCC 2 cut(s) 361, 972
XagI CCTNNNNNAGG 1 cut(s) 1220
XapI RAATTY 2 cut(s) 140, 1601
XceI RCATGY 2 cut(s) 162, 1397
XcmI CCANNNNNNNNNTGG 1 cut(s) 1645
XhoI CTCGAG 1 cut(s) 706
XmiI GTMKAC 1 cut(s) 688
XmnI GAANNNNTTC 1 cut(s) 1089
XspI CTAG 7 cut(s) 291, 458, 471, 638, 1082, 1244, 1463
ZrmI AGTACT 1 cut(s) 1183
Zsp2I ATGCAT 1 cut(s) 1808
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.