Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

OG0003616

Physical Location & Seq

Genomic Coordinates Forward (+)

3262640 .. 3267871

Gene Structure 5232 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2385 bp

ATGCATGCATGCTCCTGGGACCTTCGTAATCTCTCTCTCTCCGGAAACCTCTATCTTCCAAAGCTCCGCGCTTTCCCCCGTTTTCCTTCTCATTTTCTCGCCATCAAAACACCACCGCGTGCTTACGGACCGACCCATCAACGCCTCCGGAGTTTTGAGTTAGAGAGAAAAAGGTGCGGTGGATTTAGAGAGAGGAACAATCAGACATTTTTGGCCTTCGAGCAAGAGATGGCTTTGAGGCGCCTGATCGATCAGGTTTCAAGGCATCGATCAGAGTTGGGACAGCTGACAAAAGTGGTTGCCAGAACTTATCATCCTACCAACAGGTTCGCAGGAGGTGCCGGAGGGAACAAGTTTTTAAGTGCTCAAGAAAGGTTCAAGTCGAGCTATGTTGGCAATTTCGCTCGTAGAGTACGCGATACCAATGAAGCTAGTGAAATTTCCTATCTGAAAGAACTTGACAATCGGAGTGATTATGAAGCAGTGATAAGAGCATTTGAAAGTCAACCATCATTACATTCTAACCCTTCAGCACTTTCTGAATATGTGAAAGCATTGGTAAGAGTTGGCAGGCTGGACGAGAGTGAGTTACTCAAGACATTGCAGAGAGGTCTTGCTAACTCTGCAAGGGAGGAAGAAAGCATTGCCGGCTTCTCAGTTCTCAGGAATTTTGGAAAATCAACAAACGAAGGTATTCTTGGAACTGCAAGTGCTCCTATTCATATGGTGGCTACAGAAGGAGGTCAGTTTAAAGAGCAGCTGTGGCGTACAATTCGCACAATTGCACTGGCCTTTCTTATGATATCTGGCATTGGAGCACTCATTGAGGATAAAGGAATAAGCAAGGGACTCGGACTAAATGAGGAGGTTCAACCTAGCATGGAATCAAACACAAAATTTAGCGATGTTAAGGGTGTTGATGAGGCTAAATCCGAGTTAGAGGAAATTGTTCACTATCTTCGGGATCCTAAGCGTTTTACTTGTCTAGGTGGCAAGCTCCCAAAAGGTGTTTTGCTTGTTGGTCCACCTGGCACAGGAAAAACCATGTTAGCAAGAGCCATTGCTGGAGAAGCAGGGGTTCCTTTCTTTTCATGCAGCGGCAGTGAGTTTGAAGAGATGTATGTTGGTGTTGGGGCTCGAAGGGTGAGGGATCTTTTCTCGGCTGCAAAGAAGCGGTCACCTTGTATAATATTCATTGATGAGATAGACGCAATAGGAGGAAGCCGAAACCCAAAGGATATGCAGTACATGAAAATGACGCTTAATCAGTTACTTGTTGAACTTGATGGTTTTAAACAAAATGAAGGAATTATTGTCATTGCTGCTACTAATTTCCCAGAGTCTCTGGACAAGGCATTGGTGAGACCTGGACGGATCGATCGGCATATTGTTGTTCCAAATCCTGATGTTGAAGGGCGAAGGCAGATTATGGAAGCCCACATGTCGAAGGTTCTGAAGGCCAATGACGTAGATTTGAGTATTATTGCTAGAGGAACTCCGGGGTTCTCCGGAGCTGATCTTGCTAACTTGATCAACGTTGCTGCTCTGAAGGCTGCCATGGACGGTTCTAAAGCAGTGAATATGGTTGATCTTGAATATGCAAAAGACAAGATTATGATGGGAAGCGAACGCAAGTCAGCCGTTATATCTGATGAGTCAAGAAAGCTAACAGCTTTCCATGAGGGTGGGCATGCACTTGTAGCAATCCACACCGATGGTGCTCTTCCAGTTCACAAGGCAACAATAGTTCCTAGGGGTATGGCGCTTGGCATGGTTGCCCTGCTGCCCGAGATGGACCAGACAAGTGTCTCACGGAAACAGATGCTTGCAGAGCTCGATGTTTGTATGGGGGGGCGTGTTGCAGAAGAGCTCATCTTTGGAGAAAGTGAAGTTACTTCTGGTGCATCAAATGATCTCTGGAAAGCAACTTCGGTGGCTAGAGCAATGGTTACAAAGTACGGTATGAGCAAACAAGTTGGGCTCGTCAGTCATGATTACGACGACAACGGTAGGAGCATGAGCACAGAAACTAGGTTGCTCATCGAAGAAGAAGTGAAGTACTTATTGGAAAGGGCATACAACAATGCAAAAACCATCCTCACCGAACACAGCAAGGAACACCACGCCCTTGCTAATGCTTTAATAGAGAAGGAAACACTCACCGGAACTCAGATAAAGTCCTTGCTTGCACAAGTAAACTCCCAACAGCAGCAGCAGCAGCAACCGCACCAACAAGTAGTTGCAGCGAAGAGCGACTCACAGTCTAAGCCAGTACCGCCCTCCACCCCCAACGCGGCTGCTTCTGCCGCAGCCGCTGCATCCGCTGTTGCAGCTGCCGCAACTGCTGCAGCCAAGTCCACAGGTATTGCACCCGTCGGATCCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004176 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0009507 GO:0009526 GO:0009536 GO:0009888 GO:0009941 GO:0010073 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019538 GO:0031967 GO:0031975 GO:0032501 GO:0032502 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048507 GO:0048856 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04139 map04139

Pfam Domains

Protein Families

AAA Peptidase_M41

Protein Analysis

795

Amino Acids

86.53

Weight (kDa)

8.88

Isoelectric Point (pI)

43.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TIP49	PF06068	282 - 370	8e-06	TIP49 P-loop domain
AAA_5	PF07728	336 - 406	9.5e-06	AAA domain (dynein-related subfamily)
AAA	PF00004	337 - 466	1.1e-45	ATPase family associated with various cellular activities (AAA)
AAA_lid_3	PF17862	489 - 532	4.2e-12	AAA+ lid domain
Peptidase_M41	PF01434	548 - 728	4.5e-66	Peptidase family M41

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003616)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G26140
fragaria_vesca	FvH4_1g07720 FvH4_2g31730 FvH4_2g31730
malus_domestica	MD08G1063300.v1.1 MD15G1058100.v1.1 MD15G1058500.v1.1
prunus_persica	Prupe.1G410800_v2.0.a1
pyrus_communis	pycom08g05200 pycom15g05470 pycom15g05500
rosa_chinensis	RchiOBHm_Chr2g0093601 RchiOBHm_Chr6g0311091
rosa_laevigata	RLG00000010436 RLG00000016420
rosa_multiflora	Rmu_co8327861.1_g000001 Rmu_co8491229.1_g000001 Rmu_sc0002126.1_g000022 Rmu_sc0008967.1_g000002 Rmu_sc0012235.1_g000001
rosa_roxburghii	Rroxscaffold_2G00147960 Rroxscaffold_7G00158030
rosa_rugosa	Rorug02G0037800 Rorug02G0037800 Rorug06G0385300 Rorug06G0385400
rosa_samantha	Rh2AG084300 Rh2BG084900 Rh2CG087200 Rh2DG082900 Rh6AG499400 Rh6BG509900 Rh6CG515700 Rh6DG502300
rosa_wichuraiana	Rw6G043500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	240, 338
AccII	CGCG	4 cut(s)	69, 118, 417, 2294
AccIII	TCCGGA	3 cut(s)	41, 147, 1508
AclI	AACGTT	1 cut(s)	1536
AclWI	GGATC	5 cut(s)	959, 972, 1158, 1382, 2373
AcsI	RAATTY	3 cut(s)	438, 667, 896
AcuI	CTGAAG	3 cut(s)	513, 1475, 1568
AcyI	GRCGYC	1 cut(s)	241
AdeI	CACNNNGTG	1 cut(s)	119
AfaI	GTAC	6 cut(s)	414, 769, 1247, 1958, 2060, 2274
AfiI	CCNNNNNNNGG	2 cut(s)	1034, 2293
AflIII	ACRYGT	1 cut(s)	1440
AgsI	TTSAA	8 cut(s)	261, 379, 500, 872, 1112, 1280, 1412, 1595
AhdI	GACNNNNNGTC	1 cut(s)	2260
AjnI	CCWGG	3 cut(s)	14, 1027, 1366
AjuI	GAANNNNNNNTTGG	4 cut(s)	681, 713, 2048, 2080
AloI	GAACNNNNNNTCC	2 cut(s)	298, 330
Alw21I	GWGCWC	7 cut(s)	367, 715, 820, 1723, 1836, 1872, 2024
Alw26I	GTCTC	3 cut(s)	1347, 1357, 1813
AlwI	GGATC	5 cut(s)	959, 972, 1158, 1382, 2373
AlwNI	CAGNNNCTG	1 cut(s)	2315
Ama87I	CYCGRG	1 cut(s)	1787
Aor13HI	TCCGGA	3 cut(s)	41, 147, 1508
AoxI	GGCC	3 cut(s)	213, 789, 1458
ApoI	RAATTY	3 cut(s)	438, 667, 896
ArsI	GACNNNNNNTTYG	2 cut(s)	1160, 1192
Asp700I	GAANNNNTTC	2 cut(s)	693, 948
AspA2I	CCTAGG	1 cut(s)	1751
AspLEI	GCGC	3 cut(s)	71, 243, 1765
AspS9I	GGNCC	4 cut(s)	19, 128, 1022, 1795
AsuC2I	CCSGG	1 cut(s)	1500
AsuHPI	GGTGA	5 cut(s)	1156, 1170, 1372, 2092, 2152
AvaI	CYCGRG	1 cut(s)	1787
AvaII	GGWCC	4 cut(s)	19, 128, 1022, 1795
AvrII	CCTAGG	1 cut(s)	1751
BamHI	GGATCC	2 cut(s)	964, 2378
BanI	GGYRCC	2 cut(s)	240, 338
BanII	GRGCYC	4 cut(s)	1138, 1836, 1872, 1983
Bbv12I	GWGCWC	7 cut(s)	367, 715, 820, 1723, 1836, 1872, 2024
BccI	CCATC	9 cut(s)	110, 144, 223, 517, 1280, 1612, 1709, 1786, 2102
BceAI	ACGGC	1 cut(s)	1625
BcgI	CGANNNNNNTGC	2 cut(s)	832, 866
BciT130I	CCWGG	3 cut(s)	16, 1029, 1368
BclI	TGATCA	1 cut(s)	1530
BcnI	CCSGG	1 cut(s)	1500
BcoDI	GTCTC	3 cut(s)	1347, 1357, 1813
BfaI	CTAG	8 cut(s)	432, 876, 986, 1488, 1752, 1938, 2031, 2383
BfmI	CTRYAG	2 cut(s)	732, 2346
BfoI	RGCGCY	2 cut(s)	244, 1766
BlnI	CCTAGG	1 cut(s)	1751
BmcAI	AGTACT	1 cut(s)	2060
Bme1390I	CCNGG	4 cut(s)	16, 1029, 1368, 1500
Bme18I	GGWCC	4 cut(s)	19, 128, 1022, 1795
BmeRI	GACNNNNNGTC	1 cut(s)	2260
BmeT110I	CYCGRG	1 cut(s)	1787
BmgT120I	GGNCC	4 cut(s)	19, 128, 1022, 1795
BmiI	GGNNCC	6 cut(s)	20, 242, 340, 966, 1080, 2380
BmrFI	CCNGG	4 cut(s)	16, 1029, 1368, 1500
BmsI	GCATC	4 cut(s)	274, 1812, 1913, 2327
BoxI	GACNNNNGTC	1 cut(s)	1805
BpmI	CTGGAG	1 cut(s)	1086
Bpu10I	CCTNAGC	1 cut(s)	969
BpuEI	CTTGAG	2 cut(s)	351, 578
BpuMI	CCSGG	1 cut(s)	1500
Bsa29I	ATCGAT	3 cut(s)	249, 268, 1377
BsaHI	GRCGYC	1 cut(s)	241
BsaI	GGTCTC	1 cut(s)	1357
BsaJI	CCNNGG	4 cut(s)	15, 1499, 1557, 1751
BsaWI	WCCGGW	4 cut(s)	41, 147, 1508, 2162
Bsc4I	CCNNNNNNNGG	2 cut(s)	1034, 2293
Bse118I	RCCGGY	1 cut(s)	647
Bse1I	ACTGG	3 cut(s)	792, 1727, 2270
Bse3DI	GCAATG	5 cut(s)	599, 642, 1059, 1317, 1950
BseAI	TCCGGA	3 cut(s)	41, 147, 1508
BseBI	CCWGG	3 cut(s)	16, 1029, 1368
BseCI	ATCGAT	3 cut(s)	249, 268, 1377
BseDI	CCNNGG	4 cut(s)	15, 1499, 1557, 1751
BseGI	GGATG	3 cut(s)	313, 2094, 2318
BseLI	CCNNNNNNNGG	2 cut(s)	1034, 2293
BseMI	GCAATG	5 cut(s)	599, 642, 1059, 1317, 1950
BseMII	CTCAG	3 cut(s)	669, 676, 2183
BseNI	ACTGG	3 cut(s)	792, 1727, 2270
BseRI	GAGGAG	1 cut(s)	878
Bsh1236I	CGCG	4 cut(s)	69, 118, 417, 2294
Bsh1285I	CGRYCG	1 cut(s)	1381
BshFI	GGCC	3 cut(s)	215, 791, 1460
BshNI	GGYRCC	2 cut(s)	240, 338
BshVI	ATCGAT	3 cut(s)	249, 268, 1377
BsiEI	CGRYCG	1 cut(s)	1381
BsiHKAI	GWGCWC	7 cut(s)	367, 715, 820, 1723, 1836, 1872, 2024
BsiHKCI	CYCGRG	1 cut(s)	1787
BsiSI	CCGG	7 cut(s)	42, 148, 342, 648, 1499, 1509, 2163
BslFI	GGGAC	3 cut(s)	32, 294, 861
BslI	CCNNNNNNNGG	2 cut(s)	1034, 2293
BsmAI	GTCTC	3 cut(s)	1347, 1357, 1813
BsmFI	GGGAC	3 cut(s)	32, 294, 861
BsnI	GGCC	3 cut(s)	215, 791, 1460
Bso31I	GGTCTC	1 cut(s)	1357
BsoBI	CYCGRG	1 cut(s)	1787
Bsp1286I	GDGCHC	9 cut(s)	367, 715, 820, 1138, 1723, 1836, 1872, 1983, 2024
Bsp13I	TCCGGA	3 cut(s)	41, 147, 1508
Bsp19I	CCATGG	1 cut(s)	1557
BspANI	GGCC	3 cut(s)	215, 791, 1460
BspCNI	CTCAG	3 cut(s)	668, 675, 2182
BspDI	ATCGAT	3 cut(s)	249, 268, 1377
BspEI	TCCGGA	3 cut(s)	41, 147, 1508
BspFNI	CGCG	4 cut(s)	69, 118, 417, 2294
BspHI	TCATGA	1 cut(s)	1990
BspLI	GGNNCC	6 cut(s)	20, 242, 340, 966, 1080, 2380
BspMAI	CTGCAG	1 cut(s)	2350
BspPI	GGATC	5 cut(s)	959, 972, 1158, 1382, 2373
BspQI	GCTCTTC	3 cut(s)	1728, 1860, 2243
BspT107I	GGYRCC	2 cut(s)	240, 338
BspTNI	GGTCTC	1 cut(s)	1357
BsrDI	GCAATG	5 cut(s)	599, 642, 1059, 1317, 1950
BsrFI	RCCGGY	1 cut(s)	647
BsrI	ACTGG	3 cut(s)	792, 1727, 2270
BssAI	RCCGGY	1 cut(s)	647
BssECI	CCNNGG	4 cut(s)	15, 1499, 1557, 1751
BssNI	GRCGYC	1 cut(s)	241
BssT1I	CCWWGG	2 cut(s)	1557, 1751
Bst2UI	CCWGG	3 cut(s)	16, 1029, 1368
Bst4CI	ACNGT	4 cut(s)	1565, 1961, 2009, 2262
Bst6I	CTCTTC	4 cut(s)	1107, 1728, 1860, 2243
BstACI	GRCGYC	1 cut(s)	241
BstAPI	GCANNNNNTGC	3 cut(s)	338, 2315, 2345
BstC8I	GCNNGC	9 cut(s)	6, 10, 120, 572, 649, 995, 1692, 1827, 2187
BstDEI	CTNAG	5 cut(s)	655, 662, 969, 2169, 2265
BstDSI	CCRYGG	1 cut(s)	1557
BstEII	GGTNACC	1 cut(s)	1176
BstENI	CCTNNNNNAGG	1 cut(s)	1032
BstF5I	GGATG	3 cut(s)	313, 2094, 2318
BstFNI	CGCG	4 cut(s)	69, 118, 417, 2294
BstH2I	RGCGCY	2 cut(s)	244, 1766
BstHHI	GCGC	3 cut(s)	71, 243, 1765
BstMAI	GTCTC	3 cut(s)	1347, 1357, 1813
BstMCI	CGRYCG	1 cut(s)	1381
BstNI	CCWGG	3 cut(s)	16, 1029, 1368
BstNSI	RCATGY	4 cut(s)	8, 12, 1444, 1694
BstPAI	GACNNNNGTC	1 cut(s)	1805
BstPI	GGTNACC	1 cut(s)	1176
BstSCI	CCNGG	4 cut(s)	14, 1027, 1366, 1498
BstSFI	CTRYAG	2 cut(s)	732, 2346
BstUI	CGCG	4 cut(s)	69, 118, 417, 2294
BstX2I	RGATCY	3 cut(s)	964, 1150, 2378
BstXI	CCANNNNNNTGG	2 cut(s)	1685, 1715
BstYI	RGATCY	3 cut(s)	964, 1150, 2378
Bsu15I	ATCGAT	3 cut(s)	249, 268, 1377
BsuRI	GGCC	3 cut(s)	215, 791, 1460
BsuTUI	ATCGAT	3 cut(s)	249, 268, 1377
BtgI	CCRYGG	1 cut(s)	1557
BtgZI	GCGATG	1 cut(s)	918
BtsCI	GGATG	3 cut(s)	313, 2094, 2318
BtsI	GCAGTG	3 cut(s)	489, 1108, 1581
BtsIMutI	CAGTG	4 cut(s)	489, 785, 1108, 1581
Cac8I	GCNNGC	9 cut(s)	6, 10, 120, 572, 649, 995, 1692, 1827, 2187
CaiI	CAGNNNCTG	1 cut(s)	2315
CciI	TCATGA	1 cut(s)	1990
CfoI	GCGC	3 cut(s)	71, 243, 1765
Cfr10I	RCCGGY	1 cut(s)	647
Cfr13I	GGNCC	4 cut(s)	19, 128, 1022, 1795
ClaI	ATCGAT	3 cut(s)	249, 268, 1377
CpoI	CGGWCCG	1 cut(s)	128
CseI	GACGC	2 cut(s)	1217, 1267
Csp6I	GTAC	6 cut(s)	413, 768, 1246, 1957, 2059, 2273
CspCI	CAANNNNNGTGG	4 cut(s)	1914, 1949, 2347, 2382
CspI	CGGWCCG	1 cut(s)	128
CviQI	GTAC	6 cut(s)	413, 768, 1246, 1957, 2059, 2273
DdeI	CTNAG	5 cut(s)	655, 662, 969, 2169, 2265
DinI	GGCGCC	1 cut(s)	242
DraI	TTTAAA	2 cut(s)	751, 1294
DraIII	CACNNNGTG	1 cut(s)	119
DriI	GACNNNNNGTC	1 cut(s)	2260
Eam1104I	CTCTTC	4 cut(s)	1107, 1728, 1860, 2243
Eam1105I	GACNNNNNGTC	1 cut(s)	2260
EarI	CTCTTC	4 cut(s)	1107, 1728, 1860, 2243
Ecl136II	GAGCTC	2 cut(s)	1834, 1870
Eco130I	CCWWGG	2 cut(s)	1557, 1751
Eco24I	GRGCYC	4 cut(s)	1138, 1836, 1872, 1983
Eco31I	GGTCTC	1 cut(s)	1357
Eco32I	GATATC	1 cut(s)	804
Eco47I	GGWCC	4 cut(s)	19, 128, 1022, 1795
Eco53kI	GAGCTC	2 cut(s)	1834, 1870
Eco57I	CTGAAG	3 cut(s)	513, 1475, 1568
Eco88I	CYCGRG	1 cut(s)	1787
Eco91I	GGTNACC	1 cut(s)	1176
EcoICRI	GAGCTC	2 cut(s)	1834, 1870
EcoNI	CCTNNNNNAGG	1 cut(s)	1032
EcoO109I	RGGNCCY	1 cut(s)	19
EcoO65I	GGTNACC	1 cut(s)	1176
EcoRII	CCWGG	3 cut(s)	14, 1027, 1366
EcoRV	GATATC	1 cut(s)	804
EcoT14I	CCWWGG	2 cut(s)	1557, 1751
EcoT22I	ATGCAT	2 cut(s)	6, 10
EcoT38I	GRGCYC	4 cut(s)	1138, 1836, 1872, 1983
EgeI	GGCGCC	1 cut(s)	242
EheI	GGCGCC	1 cut(s)	242
ErhI	CCWWGG	2 cut(s)	1557, 1751
FalI	AAGNNNNNCTT	2 cut(s)	681, 713
FaqI	GGGAC	3 cut(s)	32, 294, 861
FauNDI	CATATG	1 cut(s)	723
FbaI	TGATCA	1 cut(s)	1530
FokI	GGATG	3 cut(s)	300, 2081, 2305
FriOI	GRGCYC	4 cut(s)	1138, 1836, 1872, 1983
FspBI	CTAG	8 cut(s)	432, 876, 986, 1488, 1752, 1938, 2031, 2383
GlaI	GCGC	3 cut(s)	70, 242, 1764
GsuI	CTGGAG	1 cut(s)	1086
HaeII	RGCGCY	2 cut(s)	244, 1766
HaeIII	GGCC	3 cut(s)	215, 791, 1460
HapII	CCGG	7 cut(s)	42, 148, 342, 648, 1499, 1509, 2163
HgaI	GACGC	2 cut(s)	1217, 1267
HhaI	GCGC	3 cut(s)	71, 243, 1765
Hin1I	GRCGYC	1 cut(s)	241
Hin6I	GCGC	3 cut(s)	69, 241, 1763
HinP1I	GCGC	3 cut(s)	69, 241, 1763
HincII	GTYRAC	1 cut(s)	506
HindII	GTYRAC	1 cut(s)	506
HinfI	GANTC	5 cut(s)	849, 884, 1340, 1655, 2255
HpaII	CCGG	7 cut(s)	42, 148, 342, 648, 1499, 1509, 2163
HphI	GGTGA	5 cut(s)	1156, 1170, 1372, 2092, 2152
Hpy166II	GTNNAC	6 cut(s)	506, 952, 1025, 1732, 2197, 2358
Hpy8I	GTNNAC	6 cut(s)	506, 952, 1025, 1732, 2197, 2358
Hpy99I	CGWCG	2 cut(s)	2003, 2378
HpyCH4III	ACNGT	4 cut(s)	1565, 1961, 2009, 2262
HpyCH4IV	ACGT	2 cut(s)	1467, 1536
HpyF3I	CTNAG	5 cut(s)	655, 662, 969, 2169, 2265
HpySE526I	ACGT	2 cut(s)	1467, 1536
Hsp92I	GRCGYC	1 cut(s)	241
HspAI	GCGC	3 cut(s)	69, 241, 1763
KasI	GGCGCC	1 cut(s)	240
Kpn2I	TCCGGA	3 cut(s)	41, 147, 1508
KroI	GCCGGC	1 cut(s)	647
KroNI	GCCGGC	1 cut(s)	649
Ksp22I	TGATCA	1 cut(s)	1530
LguI	GCTCTTC	3 cut(s)	1728, 1860, 2243
LmnI	GCTCC	7 cut(s)	17, 69, 718, 815, 1002, 1511, 2013
LweI	GCATC	4 cut(s)	274, 1812, 1913, 2327
MaeI	CTAG	8 cut(s)	432, 876, 986, 1488, 1752, 1938, 2031, 2383
MaeII	ACGT	2 cut(s)	1467, 1536
MaeIII	GTNAC	5 cut(s)	588, 1176, 1269, 1891, 1948
MboII	GAAGA	9 cut(s)	47, 647, 950, 1124, 1715, 1877, 2057, 2060, 2260
MfeI	CAATTG	1 cut(s)	780
MflI	RGATCY	3 cut(s)	964, 1150, 2378
MhlI	GDGCHC	9 cut(s)	367, 715, 820, 1138, 1723, 1836, 1872, 1983, 2024
MluCI	AATT	9 cut(s)	397, 438, 667, 771, 780, 896, 945, 1308, 1330
Mly113I	GGCGCC	1 cut(s)	241
MlyI	GAGTC	4 cut(s)	843, 1349, 1664, 2249
MmeI	TCCRAC	1 cut(s)	2356
Mph1103I	ATGCAT	2 cut(s)	6, 10
MroI	TCCGGA	3 cut(s)	41, 147, 1508
MroNI	GCCGGC	1 cut(s)	647
MroXI	GAANNNNTTC	2 cut(s)	693, 948
MseI	TTAA	6 cut(s)	359, 750, 909, 1263, 1293, 2141
MslI	CAYNNNNRTG	3 cut(s)	1253, 1683, 1713
MspA1I	CMGCKG	6 cut(s)	286, 760, 1098, 2315, 2324, 2333
MspI	CCGG	7 cut(s)	42, 148, 342, 648, 1499, 1509, 2163
MspR9I	CCNGG	4 cut(s)	16, 1029, 1368, 1500
MunI	CAATTG	1 cut(s)	780
MvaI	CCWGG	3 cut(s)	16, 1029, 1368
MvnI	CGCG	4 cut(s)	69, 118, 417, 2294
NaeI	GCCGGC	1 cut(s)	649
NarI	GGCGCC	1 cut(s)	241
NciI	CCSGG	1 cut(s)	1500
NcoI	CCATGG	1 cut(s)	1557
NdeI	CATATG	1 cut(s)	723
NgoMIV	GCCGGC	1 cut(s)	647
NlaIV	GGNNCC	6 cut(s)	20, 242, 340, 966, 1080, 2380
NmeAIII	GCCGAG	1 cut(s)	1139
NmuCI	GTSAC	1 cut(s)	1176
NsiI	ATGCAT	2 cut(s)	6, 10
NspI	RCATGY	4 cut(s)	8, 12, 1444, 1694
PaeI	GCATGC	3 cut(s)	8, 12, 1694
PagI	TCATGA	1 cut(s)	1990
PciI	ACATGT	1 cut(s)	1440
PciSI	GCTCTTC	3 cut(s)	1728, 1860, 2243
PdiI	GCCGGC	1 cut(s)	649
PdmI	GAANNNNTTC	2 cut(s)	693, 948
PfeI	GAWTC	1 cut(s)	884
Ple19I	CGATCG	1 cut(s)	1381
PleI	GAGTC	4 cut(s)	843, 1348, 1663, 2249
PluTI	GGCGCC	1 cut(s)	244
PpsI	GAGTC	4 cut(s)	843, 1348, 1663, 2249
PpuMI	RGGWCCY	1 cut(s)	19
PscI	ACATGT	1 cut(s)	1440
PshAI	GACNNNNGTC	1 cut(s)	1805
Psp124BI	GAGCTC	2 cut(s)	1836, 1872
Psp1406I	AACGTT	1 cut(s)	1536
Psp5II	RGGWCCY	1 cut(s)	19
Psp6I	CCWGG	3 cut(s)	14, 1027, 1366
PspEI	GGTNACC	1 cut(s)	1176
PspGI	CCWGG	3 cut(s)	14, 1027, 1366
PspN4I	GGNNCC	6 cut(s)	20, 242, 340, 966, 1080, 2380
PspPI	GGNCC	4 cut(s)	19, 128, 1022, 1795
PspPPI	RGGWCCY	1 cut(s)	19
PstI	CTGCAG	1 cut(s)	2350
PstNI	CAGNNNCTG	1 cut(s)	2315
PsuI	RGATCY	3 cut(s)	964, 1150, 2378
PvuI	CGATCG	1 cut(s)	1381
PvuII	CAGCTG	3 cut(s)	286, 760, 2333
RsaI	GTAC	6 cut(s)	414, 769, 1247, 1958, 2060, 2274
RsaNI	GTAC	6 cut(s)	413, 768, 1246, 1957, 2059, 2273
RseI	CAYNNNNRTG	3 cut(s)	1253, 1683, 1713
Rsr2I	CGGWCCG	1 cut(s)	128
RsrII	CGGWCCG	1 cut(s)	128
SacI	GAGCTC	2 cut(s)	1836, 1872
SapI	GCTCTTC	3 cut(s)	1728, 1860, 2243
SaqAI	TTAA	6 cut(s)	359, 750, 909, 1263, 1293, 2141
Sau96I	GGNCC	4 cut(s)	19, 128, 1022, 1795
ScaI	AGTACT	1 cut(s)	2060
SchI	GAGTC	4 cut(s)	843, 1349, 1664, 2249
ScrFI	CCNGG	4 cut(s)	16, 1029, 1368, 1500
SduI	GDGCHC	9 cut(s)	367, 715, 820, 1138, 1723, 1836, 1872, 1983, 2024
SfaNI	GCATC	4 cut(s)	274, 1812, 1913, 2327
SfcI	CTRYAG	2 cut(s)	732, 2346
SfoI	GGCGCC	1 cut(s)	242
SinI	GGWCC	4 cut(s)	19, 128, 1022, 1795
SmiMI	CAYNNNNRTG	3 cut(s)	1253, 1683, 1713
SmlI	CTYRAG	2 cut(s)	366, 593
SmoI	CTYRAG	2 cut(s)	366, 593
SphI	GCATGC	3 cut(s)	8, 12, 1694
Sse9I	AATT	9 cut(s)	397, 438, 667, 771, 780, 896, 945, 1308, 1330
SspDI	GGCGCC	1 cut(s)	240
SspI	AATATT	1 cut(s)	1191
SspMI	CTAG	8 cut(s)	432, 876, 986, 1488, 1752, 1938, 2031, 2383
SstI	GAGCTC	2 cut(s)	1836, 1872
StyD4I	CCNGG	4 cut(s)	14, 1027, 1366, 1498
StyI	CCWWGG	2 cut(s)	1557, 1751
TaaI	ACNGT	4 cut(s)	1565, 1961, 2009, 2262
TaiI	ACGT	2 cut(s)	1470, 1539
TaqI	TCGA	9 cut(s)	219, 249, 268, 383, 1138, 1377, 1445, 1836, 2043
TaqII	GACCGA	1 cut(s)	145
TasI	AATT	9 cut(s)	397, 438, 667, 771, 780, 896, 945, 1308, 1330
TatI	WGTACW	2 cut(s)	1245, 2058
TauI	GCSGC	5 cut(s)	1101, 2297, 2309, 2315, 2339
TfiI	GAWTC	1 cut(s)	884
Tru1I	TTAA	6 cut(s)	359, 750, 909, 1263, 1293, 2141
Tru9I	TTAA	6 cut(s)	359, 750, 909, 1263, 1293, 2141
TscAI	CASTG	4 cut(s)	489, 792, 1108, 1581
TseFI	GTSAC	1 cut(s)	1176
Tsp45I	GTSAC	1 cut(s)	1176
TspDTI	ATGAA	7 cut(s)	441, 492, 710, 1080, 1183, 1265, 1317
TspGWI	ACGGA	3 cut(s)	141, 1387, 1828
TspRI	CASTG	4 cut(s)	489, 792, 1108, 1581
VpaK11BI	GGWCC	4 cut(s)	19, 128, 1022, 1795
XagI	CCTNNNNNAGG	1 cut(s)	1032
XapI	RAATTY	3 cut(s)	438, 667, 896
XceI	RCATGY	4 cut(s)	8, 12, 1444, 1694
XmaJI	CCTAGG	1 cut(s)	1751
XmnI	GAANNNNTTC	2 cut(s)	693, 948
XspI	CTAG	8 cut(s)	432, 876, 986, 1488, 1752, 1938, 2031, 2383
ZrmI	AGTACT	1 cut(s)	2060
Zsp2I	ATGCAT	2 cut(s)	6, 10

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.