Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

OG0003616

Physical Location & Seq

Genomic Coordinates Forward (+)

3276855 .. 3283422

Gene Structure 6568 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2385 bp

ATGACTTTTGCATCTCTGTATGGCCACGAAGAATTTTTCCCACTTCCAAAGATCTTTTTCCTTCCAAATCCCCGGTCTTTCCCCCGTTTTCCTTCTCATTTTCTCGCCATCCAAACGCAACCGCGAGCTTACGGACCGACCCATCAAAGCCTCCGGAGTTTTGAGTTAGAGAGAGAAAGGCGCGGTGGATTTAGTGCGAGAAACAATCAGACATTGTTGGTCTTCCAGCAAGAGATGGCTTTGAGGCGCCTGATCAATCAGGTTTCAAGGCATCGATCAGAGTTGGGACAACTGACAAAAGTGGTTACCAGAACTTATCATCCTACCAACAGGTTACGAGGAGGTGCCGGAGGGAATAAGTTTTTAAGTACTCAAGAAAGATTCAAGTCCAGTTATGTTGCCAATTTCGGCCGTAGGGTACGCGATACCGATGAAGCTAGTGAAATTTCCTATCTAAAAGAACTTTACCATCGGAGTGACTATGAAGCAGTGATAAGAGCATTTGAAAGTCAACCTTCATTACATTCTAACCCTTCAGCACTTTCTGAATATGTGAAAGCATTAGTAAGAGTTGGCAGGCTGGATGACAGTGGTTTACTCAAGACATTGCAGAGAGGTGTTGCTAACTCTGCAAGGGAAGAAGAAAGCATTGCCGGCTTCTCAGTTCTCAAGAATTTTGGAAAAACTACGAAAGAAGGTATTCTTGGAACTGCAAATGCTCCTATTCATATGGTGGCTACAGAAGGTGGTCAGTTTAAAGAGCAGCTGTGGCGTACAATTCGCTCTATCGCATTGGCCTTTCTTTTGATATCTGGTATAGGAGCACTCATTGAGGATAAAGGAATAAGCAAAGGACTCGGACTAAATGAGGAGGTTCAACCTATCACAGAATCAAGCACAAAATTTAGTGATGTAAAGGGTGTTGATGAGGCTAAATCTGAGTTAGAGGAAATTGTTCACTATCTTCGAGATCCTAAGCGTTTCACTTGTCTAGGTGGCAAGCTCCCAAAAGGTGTCTTGCTTGTTGGCCCACCTGGCACTGGGAAAACCATGTTAGCAAGAGCCATTGCTGGAGAAGCCGGGGTACCTTTCTTTTCATGCAGCGGCAGTGAGTTTGAAGAGATGTTTGTTGGTGTAGGGGCTCGAAGGGTAAGGGATCTTTTCTCGGCTGCAAAGAAGCAGTCACCTTGTATAATATTCATTGATGAGATAGACGTAATAGGAGGAAGCCGAAACCCAATGGATCAGAAGTACATGAAGATGACCCTCAATCAGTTACTTGTCGAACTTGATGGTTTTAAACAAAATGAAGGGATTATTGTCATTGCTGCTACTAATTTCCCAGAATCTCTGGACAAGGCATTGGTGAGACCTGGACGGTTTGATCGGCACATTGTTGTCCCTAATCCTGATGTTGAAGGGCGAAGGCAGATTATGGAAGCCCACATGTCGAAGGTTCTGAAGGCCAGTGACGTCGATTTGAGTATTATTGCCAGAGGAACTCCGGGGTTCTCTGGAGCTGATCTTGCGAACTTGATCAACATTGCTGCTCTGAAAGCTGCCATGGACGGTGCTAAAGCAGTGAACATGGCTGATCTTGAATATGCAAAAGACAAAATTATGATGGGAAGCGAACGCAAGTCAGCTGTTATATCTGATGAGTCAAGAAAGGTAACAGCTTTCCATGAGGGTGGCCATGCACTTGTAGCAATCCACACTGATGGTGCTCATCCAGTTCACAAGGCAACAATAGTTCCTAGGGGTATGGCGCTTGGCATGGTTATGCAGCTGCCCGACAAGGACCAGACAAGTGTCTCCCGGAAACAGATGCTAGCACGGCTTGATGTTTGTATGGGGGGGCGTGTTGCAGAAGAGCTCATTTTTGGAGAAAGTGAAGTTACGTCCGGTGCATCAAATGATCTCCAGCAAGCAACTTCGCTTGCTAGAGCAATGGTTACGAAGTACGGCATGAGCAAACAAGTTGGGCTCGTCAGTCACAATTACGACGACAATGGTAAGAGCTTGAGCACAGAAACCAGGCTGCTCATCGAAGAAGAAGTGAAGTACTTATTGGAAAGGGCATACGACAATGCAAAAGCCATCCTCACCGAACACAGCAAGGAACATTACGCACTTGCTAATGCTTTACTGGAGGAGGAAACACTCACTGGAACTCAGATCAAATCCTTGCTTGCACAAGTAAGCTCCCAGCAACAGCAGCAACCGTACCAGCAAGTAGTTGCAGCGAAGAGCAACTCGCAGTCTAAGCCAGTGCCACCCTCCACCCCCAACGCAGCTGCATCTGCCGCTGCGGCTGCATCCGCCGCTGCCGCCGCCGCAACTGCTGCGGCCAAGTCAACAGGTATTGCTCCTGTTGGATCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004176 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0009507 GO:0009526 GO:0009536 GO:0009888 GO:0009941 GO:0010073 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019538 GO:0031967 GO:0031975 GO:0032501 GO:0032502 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048507 GO:0048856 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04139 map04139

Pfam Domains

Protein Families

AAA Peptidase_M41

Protein Analysis

795

Amino Acids

86.46

Weight (kDa)

8.94

Isoelectric Point (pI)

43.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AAA_5	PF07728	338 - 456	2.8e-07	AAA domain (dynein-related subfamily)
AAA	PF00004	339 - 468	3.1e-45	ATPase family associated with various cellular activities (AAA)
AAA_lid_3	PF17862	491 - 534	3.1e-12	AAA+ lid domain
Peptidase_M41	PF01434	550 - 730	5.1e-67	Peptidase family M41

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0003616)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G26140
fragaria_vesca	FvH4_1g07720 FvH4_2g31730 FvH4_2g31730
malus_domestica	MD08G1063300.v1.1 MD15G1058100.v1.1 MD15G1058500.v1.1
prunus_persica	Prupe.1G410800_v2.0.a1
pyrus_communis	pycom08g05200 pycom15g05470 pycom15g05500
rosa_chinensis	RchiOBHm_Chr2g0093601 RchiOBHm_Chr6g0311091
rosa_laevigata	RLG00000010436 RLG00000016420
rosa_multiflora	Rmu_co8327861.1_g000001 Rmu_co8491229.1_g000001 Rmu_sc0002126.1_g000022 Rmu_sc0008967.1_g000002 Rmu_sc0012235.1_g000001
rosa_roxburghii	Rroxscaffold_2G00147960 Rroxscaffold_7G00158030
rosa_rugosa	Rorug02G0037800 Rorug02G0037800 Rorug06G0385300 Rorug06G0385400
rosa_samantha	Rh2AG084300 Rh2BG084900 Rh2CG087200 Rh2DG082900 Rh6AG499400 Rh6BG509900 Rh6CG515700 Rh6DG502300
rosa_wichuraiana	Rw6G043500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1476
Acc65I	GGTACC	1 cut(s)	1084
AccB1I	GGYRCC	3 cut(s)	246, 344, 1084
AccII	CGCG	3 cut(s)	124, 183, 423
AccIII	TCCGGA	1 cut(s)	153
AclWI	GGATC	3 cut(s)	965, 1164, 1251
AcoI	YGGCCR	4 cut(s)	22, 409, 1693, 2349
AcsI	RAATTY	4 cut(s)	32, 444, 673, 902
AcuI	CTGAAG	2 cut(s)	519, 1481
AcyI	GRCGYC	2 cut(s)	247, 1473
AfaI	GTAC	8 cut(s)	370, 420, 775, 1086, 1253, 1964, 2066, 2228
AfiI	CCNNNNNNNGG	1 cut(s)	1040
AflIII	ACRYGT	1 cut(s)	1446
AgsI	TTSAA	7 cut(s)	267, 385, 506, 878, 1118, 1418, 1601
AjnI	CCWGG	3 cut(s)	1033, 1372, 2036
AjuI	GAANNNNNNNTTGG	4 cut(s)	687, 719, 2054, 2086
AloI	GAACNNNNNNTCC	2 cut(s)	304, 336
Alw21I	GWGCWC	4 cut(s)	826, 1729, 1878, 2030
Alw26I	GTCTC	2 cut(s)	1363, 1819
AlwI	GGATC	3 cut(s)	965, 1164, 1251
Aor13HI	TCCGGA	1 cut(s)	153
AoxI	GGCC	7 cut(s)	22, 409, 795, 1027, 1464, 1693, 2349
ApoI	RAATTY	4 cut(s)	32, 444, 673, 902
ArsI	GACNNNNNNTTYG	4 cut(s)	59, 91, 1166, 1198
Asp700I	GAANNNNTTC	2 cut(s)	699, 954
Asp718I	GGTACC	1 cut(s)	1084
AspA2I	CCTAGG	1 cut(s)	1757
AspLEI	GCGC	3 cut(s)	183, 249, 1771
AspS9I	GGNCC	3 cut(s)	134, 1028, 1801
AsuC2I	CCSGG	4 cut(s)	73, 1081, 1506, 1819
AsuHPI	GGTGA	3 cut(s)	1176, 1378, 2098
AsuNHI	GCTAGC	1 cut(s)	1831
AvaII	GGWCC	2 cut(s)	134, 1801
AvrII	CCTAGG	1 cut(s)	1757
BalI	TGGCCA	2 cut(s)	24, 1695
BanI	GGYRCC	3 cut(s)	246, 344, 1084
BanII	GRGCYC	3 cut(s)	1144, 1878, 1989
BarI	GAAGNNNNNNTAC	2 cut(s)	1068, 1100
BbsI	GAAGAC	1 cut(s)	214
Bbv12I	GWGCWC	4 cut(s)	826, 1729, 1878, 2030
BccI	CCATC	8 cut(s)	116, 150, 229, 477, 1286, 1618, 1715, 2108
BceAI	ACGGC	3 cut(s)	396, 1853, 1981
BcgI	CGANNNNNNTGC	2 cut(s)	838, 872
BciT130I	CCWGG	3 cut(s)	1035, 1374, 2038
BclI	TGATCA	2 cut(s)	252, 1536
BcnI	CCSGG	4 cut(s)	73, 1081, 1506, 1819
BcoDI	GTCTC	2 cut(s)	1363, 1819
BfaI	CTAG	5 cut(s)	438, 992, 1758, 1832, 1944
BfmI	CTRYAG	1 cut(s)	738
BfoI	RGCGCY	2 cut(s)	250, 1772
BglI	GCCNNNNNGGC	2 cut(s)	1035, 2312
BglII	AGATCT	1 cut(s)	51
BlnI	CCTAGG	1 cut(s)	1757
BmcAI	AGTACT	2 cut(s)	370, 2066
Bme1390I	CCNGG	7 cut(s)	73, 1035, 1081, 1374, 1506, 1819, 2038
Bme18I	GGWCC	2 cut(s)	134, 1801
BmgT120I	GGNCC	3 cut(s)	134, 1028, 1801
BmiI	GGNNCC	3 cut(s)	248, 346, 1086
BmrFI	CCNGG	7 cut(s)	73, 1035, 1081, 1374, 1506, 1819, 2038
BmrI	ACTGGG	1 cut(s)	1050
BmsI	GCATC	6 cut(s)	20, 280, 1818, 1919, 2309, 2327
BmtI	GCTAGC	1 cut(s)	1835
BmuI	ACTGGG	1 cut(s)	1050
BoxI	GACNNNNGTC	1 cut(s)	1811
BpiI	GAAGAC	1 cut(s)	214
BpmI	CTGGAG	4 cut(s)	1092, 1536, 1907, 2171
Bpu10I	CCTNAGC	1 cut(s)	975
BpuEI	CTTGAG	4 cut(s)	357, 584, 653, 2044
BpuMI	CCSGG	4 cut(s)	73, 1081, 1506, 1819
Bsa29I	ATCGAT	1 cut(s)	274
BsaHI	GRCGYC	2 cut(s)	247, 1473
BsaI	GGTCTC	1 cut(s)	1363
BsaJI	CCNNGG	5 cut(s)	71, 1080, 1505, 1563, 1757
BsaWI	WCCGGW	2 cut(s)	153, 1904
Bsc4I	CCNNNNNNNGG	1 cut(s)	1040
Bse118I	RCCGGY	1 cut(s)	653
Bse1I	ACTGG	7 cut(s)	390, 1045, 1467, 1733, 2154, 2173, 2270
Bse3DI	GCAATG	6 cut(s)	605, 648, 1065, 1323, 1542, 1956
BseAI	TCCGGA	1 cut(s)	153
BseBI	CCWGG	3 cut(s)	1035, 1374, 2038
BseCI	ATCGAT	1 cut(s)	274
BseDI	CCNNGG	5 cut(s)	71, 1080, 1505, 1563, 1757
BseGI	GGATG	6 cut(s)	108, 319, 589, 1729, 2100, 2318
BseLI	CCNNNNNNNGG	1 cut(s)	1040
BseMI	GCAATG	6 cut(s)	605, 648, 1065, 1323, 1542, 1956
BseMII	CTCAG	3 cut(s)	675, 930, 2189
BseNI	ACTGG	7 cut(s)	390, 1045, 1467, 1733, 2154, 2173, 2270
BseRI	GAGGAG	3 cut(s)	354, 884, 2168
BseX3I	CGGCCG	1 cut(s)	409
BseYI	CCCAGC	1 cut(s)	2208
Bsh1236I	CGCG	3 cut(s)	124, 183, 423
Bsh1285I	CGRYCG	1 cut(s)	412
BshFI	GGCC	7 cut(s)	24, 411, 797, 1029, 1466, 1695, 2351
BshNI	GGYRCC	3 cut(s)	246, 344, 1084
BshVI	ATCGAT	1 cut(s)	274
BsiEI	CGRYCG	1 cut(s)	412
BsiHKAI	GWGCWC	4 cut(s)	826, 1729, 1878, 2030
BsiSI	CCGG	8 cut(s)	73, 154, 348, 654, 1080, 1505, 1819, 1905
BslFI	GGGAC	2 cut(s)	300, 1385
BslI	CCNNNNNNNGG	1 cut(s)	1040
BsmAI	GTCTC	2 cut(s)	1363, 1819
BsmFI	GGGAC	2 cut(s)	300, 1385
BsnI	GGCC	7 cut(s)	24, 411, 797, 1029, 1466, 1695, 2351
Bso31I	GGTCTC	1 cut(s)	1363
Bsp1286I	GDGCHC	6 cut(s)	826, 1144, 1729, 1878, 1989, 2030
Bsp13I	TCCGGA	1 cut(s)	153
Bsp19I	CCATGG	1 cut(s)	1563
BspANI	GGCC	7 cut(s)	24, 411, 797, 1029, 1466, 1695, 2351
BspCNI	CTCAG	3 cut(s)	674, 931, 2188
BspDI	ATCGAT	1 cut(s)	274
BspEI	TCCGGA	1 cut(s)	153
BspFNI	CGCG	3 cut(s)	124, 183, 423
BspLI	GGNNCC	3 cut(s)	248, 346, 1086
BspOI	GCTAGC	1 cut(s)	1835
BspPI	GGATC	3 cut(s)	965, 1164, 1251
BspQI	GCTCTTC	2 cut(s)	1866, 2243
BspT107I	GGYRCC	3 cut(s)	246, 344, 1084
BspTNI	GGTCTC	1 cut(s)	1363
BsrDI	GCAATG	6 cut(s)	605, 648, 1065, 1323, 1542, 1956
BsrFI	RCCGGY	1 cut(s)	653
BsrI	ACTGG	7 cut(s)	390, 1045, 1467, 1733, 2154, 2173, 2270
BssAI	RCCGGY	1 cut(s)	653
BssECI	CCNNGG	5 cut(s)	71, 1080, 1505, 1563, 1757
BssNI	GRCGYC	2 cut(s)	247, 1473
BssT1I	CCWWGG	2 cut(s)	1563, 1757
Bst2UI	CCWGG	3 cut(s)	1035, 1374, 2038
Bst4CI	ACNGT	4 cut(s)	590, 1380, 1571, 2226
Bst6I	CTCTTC	3 cut(s)	1113, 1866, 2243
BstACI	GRCGYC	2 cut(s)	247, 1473
BstAPI	GCANNNNNTGC	1 cut(s)	2345
BstC8I	GCNNGC	8 cut(s)	126, 578, 655, 1001, 1833, 1929, 1941, 2193
BstDEI	CTNAG	6 cut(s)	661, 939, 975, 2175, 2265, 2382
BstDSI	CCRYGG	1 cut(s)	1563
BstEII	GGTNACC	1 cut(s)	304
BstF5I	GGATG	6 cut(s)	108, 319, 589, 1729, 2100, 2318
BstFNI	CGCG	3 cut(s)	124, 183, 423
BstH2I	RGCGCY	2 cut(s)	250, 1772
BstHHI	GCGC	3 cut(s)	183, 249, 1771
BstMAI	GTCTC	2 cut(s)	1363, 1819
BstMCI	CGRYCG	1 cut(s)	412
BstNI	CCWGG	3 cut(s)	1035, 1374, 2038
BstNSI	RCATGY	1 cut(s)	1450
BstPAI	GACNNNNGTC	1 cut(s)	1811
BstPI	GGTNACC	1 cut(s)	304
BstSCI	CCNGG	7 cut(s)	71, 1033, 1079, 1372, 1504, 1817, 2036
BstSFI	CTRYAG	1 cut(s)	738
BstUI	CGCG	3 cut(s)	124, 183, 423
BstV2I	GAAGAC	1 cut(s)	214
BstX2I	RGATCY	4 cut(s)	51, 970, 1156, 2378
BstXI	CCANNNNNNTGG	2 cut(s)	1691, 1721
BstYI	RGATCY	4 cut(s)	51, 970, 1156, 2378
BstZI	CGGCCG	1 cut(s)	409
Bsu15I	ATCGAT	1 cut(s)	274
BsuRI	GGCC	7 cut(s)	24, 411, 797, 1029, 1466, 1695, 2351
BsuTUI	ATCGAT	1 cut(s)	274
BtgI	CCRYGG	1 cut(s)	1563
BtsCI	GGATG	6 cut(s)	108, 319, 589, 1729, 2100, 2318
BtsI	GCAGTG	3 cut(s)	495, 1114, 1587
BtsIMutI	CAGTG	9 cut(s)	495, 595, 1038, 1114, 1474, 1587, 1716, 2166, 2277
Cac8I	GCNNGC	8 cut(s)	126, 578, 655, 1001, 1833, 1929, 1941, 2193
CfoI	GCGC	3 cut(s)	183, 249, 1771
Cfr10I	RCCGGY	1 cut(s)	653
Cfr13I	GGNCC	3 cut(s)	134, 1028, 1801
ClaI	ATCGAT	1 cut(s)	274
CpoI	CGGWCCG	1 cut(s)	134
Csp6I	GTAC	8 cut(s)	369, 419, 774, 1085, 1252, 1963, 2065, 2227
CspI	CGGWCCG	1 cut(s)	134
CviQI	GTAC	8 cut(s)	369, 419, 774, 1085, 1252, 1963, 2065, 2227
DdeI	CTNAG	6 cut(s)	661, 939, 975, 2175, 2265, 2382
DinI	GGCGCC	1 cut(s)	248
DraI	TTTAAA	2 cut(s)	757, 1300
EaeI	YGGCCR	4 cut(s)	22, 409, 1693, 2349
EagI	CGGCCG	1 cut(s)	409
Eam1104I	CTCTTC	3 cut(s)	1113, 1866, 2243
EarI	CTCTTC	3 cut(s)	1113, 1866, 2243
EciI	GGCGGA	1 cut(s)	2311
Ecl136II	GAGCTC	1 cut(s)	1876
EclXI	CGGCCG	1 cut(s)	409
Eco130I	CCWWGG	2 cut(s)	1563, 1757
Eco24I	GRGCYC	3 cut(s)	1144, 1878, 1989
Eco31I	GGTCTC	1 cut(s)	1363
Eco32I	GATATC	1 cut(s)	810
Eco47I	GGWCC	2 cut(s)	134, 1801
Eco52I	CGGCCG	1 cut(s)	409
Eco53kI	GAGCTC	1 cut(s)	1876
Eco57I	CTGAAG	2 cut(s)	519, 1481
Eco91I	GGTNACC	1 cut(s)	304
EcoICRI	GAGCTC	1 cut(s)	1876
EcoO65I	GGTNACC	1 cut(s)	304
EcoRII	CCWGG	3 cut(s)	1033, 1372, 2036
EcoRV	GATATC	1 cut(s)	810
EcoT14I	CCWWGG	2 cut(s)	1563, 1757
EcoT38I	GRGCYC	3 cut(s)	1144, 1878, 1989
EgeI	GGCGCC	1 cut(s)	248
EheI	GGCGCC	1 cut(s)	248
ErhI	CCWWGG	2 cut(s)	1563, 1757
FalI	AAGNNNNNCTT	4 cut(s)	499, 531, 687, 719
FaqI	GGGAC	2 cut(s)	300, 1385
FauNDI	CATATG	1 cut(s)	729
FbaI	TGATCA	2 cut(s)	252, 1536
FokI	GGATG	6 cut(s)	95, 306, 596, 1716, 2087, 2305
FriOI	GRGCYC	3 cut(s)	1144, 1878, 1989
FspBI	CTAG	5 cut(s)	438, 992, 1758, 1832, 1944
GlaI	GCGC	3 cut(s)	182, 248, 1770
GsaI	CCCAGC	1 cut(s)	2212
GsuI	CTGGAG	4 cut(s)	1092, 1536, 1907, 2171
HaeII	RGCGCY	2 cut(s)	250, 1772
HaeIII	GGCC	7 cut(s)	24, 411, 797, 1029, 1466, 1695, 2351
HapII	CCGG	8 cut(s)	73, 154, 348, 654, 1080, 1505, 1819, 1905
HhaI	GCGC	3 cut(s)	183, 249, 1771
Hin1I	GRCGYC	2 cut(s)	247, 1473
Hin6I	GCGC	3 cut(s)	181, 247, 1769
HinP1I	GCGC	3 cut(s)	181, 247, 1769
HincII	GTYRAC	2 cut(s)	512, 2358
HindII	GTYRAC	2 cut(s)	512, 2358
HinfI	GANTC	5 cut(s)	381, 855, 890, 1346, 1661
HpaII	CCGG	8 cut(s)	73, 154, 348, 654, 1080, 1505, 1819, 1905
HphI	GGTGA	3 cut(s)	1176, 1378, 2098
Hpy166II	GTNNAC	6 cut(s)	512, 596, 958, 1585, 1738, 2358
Hpy8I	GTNNAC	6 cut(s)	512, 596, 958, 1585, 1738, 2358
Hpy99I	CGWCG	2 cut(s)	1478, 2009
HpyCH4III	ACNGT	4 cut(s)	590, 1380, 1571, 2226
HpyCH4IV	ACGT	3 cut(s)	1215, 1473, 1901
HpyF3I	CTNAG	6 cut(s)	661, 939, 975, 2175, 2265, 2382
HpySE526I	ACGT	3 cut(s)	1215, 1473, 1901
Hsp92I	GRCGYC	2 cut(s)	247, 1473
HspAI	GCGC	3 cut(s)	181, 247, 1769
KasI	GGCGCC	1 cut(s)	246
Kpn2I	TCCGGA	1 cut(s)	153
KpnI	GGTACC	1 cut(s)	1088
KroI	GCCGGC	1 cut(s)	653
KroNI	GCCGGC	1 cut(s)	655
Ksp22I	TGATCA	2 cut(s)	252, 1536
LguI	GCTCTTC	2 cut(s)	1866, 2243
LmnI	GCTCC	6 cut(s)	724, 821, 1008, 1517, 2210, 2374
LweI	GCATC	6 cut(s)	20, 280, 1818, 1919, 2309, 2327
MaeI	CTAG	5 cut(s)	438, 992, 1758, 1832, 1944
MaeII	ACGT	3 cut(s)	1215, 1473, 1901
MflI	RGATCY	4 cut(s)	51, 970, 1156, 2378
MhlI	GDGCHC	6 cut(s)	826, 1144, 1729, 1878, 1989, 2030
MlsI	TGGCCA	2 cut(s)	24, 1695
MluNI	TGGCCA	2 cut(s)	24, 1695
Mly113I	GGCGCC	1 cut(s)	247
MlyI	GAGTC	2 cut(s)	849, 1670
MmeI	TCCRAC	1 cut(s)	2356
Mox20I	TGGCCA	2 cut(s)	24, 1695
MroI	TCCGGA	1 cut(s)	153
MroNI	GCCGGC	1 cut(s)	653
MroXI	GAANNNNTTC	2 cut(s)	699, 954
MscI	TGGCCA	2 cut(s)	24, 1695
MseI	TTAA	3 cut(s)	365, 756, 1299
MslI	CAYNNNNRTG	5 cut(s)	474, 1259, 1689, 1719, 1781
Msp20I	TGGCCA	2 cut(s)	24, 1695
MspA1I	CMGCKG	7 cut(s)	766, 1104, 1646, 1789, 2297, 2309, 2327
MspI	CCGG	8 cut(s)	73, 154, 348, 654, 1080, 1505, 1819, 1905
MspR9I	CCNGG	7 cut(s)	73, 1035, 1081, 1374, 1506, 1819, 2038
MvaI	CCWGG	3 cut(s)	1035, 1374, 2038
MvnI	CGCG	3 cut(s)	124, 183, 423
NaeI	GCCGGC	1 cut(s)	655
NarI	GGCGCC	1 cut(s)	247
NciI	CCSGG	4 cut(s)	73, 1081, 1506, 1819
NcoI	CCATGG	1 cut(s)	1563
NdeI	CATATG	1 cut(s)	729
NgoMIV	GCCGGC	1 cut(s)	653
NheI	GCTAGC	1 cut(s)	1831
NlaIV	GGNNCC	3 cut(s)	248, 346, 1086
NmeAIII	GCCGAG	1 cut(s)	1145
NmuCI	GTSAC	4 cut(s)	476, 1182, 1469, 1994
NspI	RCATGY	1 cut(s)	1450
PciI	ACATGT	1 cut(s)	1446
PciSI	GCTCTTC	2 cut(s)	1866, 2243
PdiI	GCCGGC	1 cut(s)	655
PdmI	GAANNNNTTC	2 cut(s)	699, 954
PfeI	GAWTC	3 cut(s)	381, 890, 1346
PfoI	TCCNGGA	1 cut(s)	1817
PleI	GAGTC	2 cut(s)	849, 1669
PluTI	GGCGCC	1 cut(s)	250
PpsI	GAGTC	2 cut(s)	849, 1669
PscI	ACATGT	1 cut(s)	1446
PshAI	GACNNNNGTC	1 cut(s)	1811
Psp124BI	GAGCTC	1 cut(s)	1878
Psp6I	CCWGG	3 cut(s)	1033, 1372, 2036
PspEI	GGTNACC	1 cut(s)	304
PspFI	CCCAGC	1 cut(s)	2208
PspGI	CCWGG	3 cut(s)	1033, 1372, 2036
PspN4I	GGNNCC	3 cut(s)	248, 346, 1086
PspPI	GGNCC	3 cut(s)	134, 1028, 1801
PsuI	RGATCY	4 cut(s)	51, 970, 1156, 2378
PvuII	CAGCTG	4 cut(s)	766, 1646, 1789, 2297
RsaI	GTAC	8 cut(s)	370, 420, 775, 1086, 1253, 1964, 2066, 2228
RsaNI	GTAC	8 cut(s)	369, 419, 774, 1085, 1252, 1963, 2065, 2227
RseI	CAYNNNNRTG	5 cut(s)	474, 1259, 1689, 1719, 1781
Rsr2I	CGGWCCG	1 cut(s)	134
RsrII	CGGWCCG	1 cut(s)	134
SacI	GAGCTC	1 cut(s)	1878
SapI	GCTCTTC	2 cut(s)	1866, 2243
SaqAI	TTAA	3 cut(s)	365, 756, 1299
Sau96I	GGNCC	3 cut(s)	134, 1028, 1801
ScaI	AGTACT	2 cut(s)	370, 2066
SchI	GAGTC	2 cut(s)	849, 1670
ScrFI	CCNGG	7 cut(s)	73, 1035, 1081, 1374, 1506, 1819, 2038
SduI	GDGCHC	6 cut(s)	826, 1144, 1729, 1878, 1989, 2030
SfaNI	GCATC	6 cut(s)	20, 280, 1818, 1919, 2309, 2327
SfcI	CTRYAG	1 cut(s)	738
SfoI	GGCGCC	1 cut(s)	248
SinI	GGWCC	2 cut(s)	134, 1801
SmiMI	CAYNNNNRTG	5 cut(s)	474, 1259, 1689, 1719, 1781
SmlI	CTYRAG	4 cut(s)	372, 599, 668, 2023
SmoI	CTYRAG	4 cut(s)	372, 599, 668, 2023
SspDI	GGCGCC	1 cut(s)	246
SspI	AATATT	1 cut(s)	1197
SspMI	CTAG	5 cut(s)	438, 992, 1758, 1832, 1944
SstI	GAGCTC	1 cut(s)	1878
StyD4I	CCNGG	7 cut(s)	71, 1033, 1079, 1372, 1504, 1817, 2036
StyI	CCWWGG	2 cut(s)	1563, 1757
TaaI	ACNGT	4 cut(s)	590, 1380, 1571, 2226
TaiI	ACGT	3 cut(s)	1218, 1476, 1904
TaqI	TCGA	7 cut(s)	274, 967, 1144, 1284, 1451, 1476, 2049
TaqII	GACCGA	1 cut(s)	151
TatI	WGTACW	3 cut(s)	368, 1251, 2064
TauI	GCSGC	8 cut(s)	1107, 2309, 2315, 2327, 2333, 2336, 2339, 2351
TfiI	GAWTC	3 cut(s)	381, 890, 1346
Tru1I	TTAA	3 cut(s)	365, 756, 1299
Tru9I	TTAA	3 cut(s)	365, 756, 1299
TscAI	CASTG	9 cut(s)	495, 595, 1045, 1114, 1474, 1587, 1723, 2173, 2277
TseFI	GTSAC	4 cut(s)	476, 1182, 1469, 1994
Tsp45I	GTSAC	4 cut(s)	476, 1182, 1469, 1994
TspDTI	ATGAA	8 cut(s)	447, 498, 507, 716, 1086, 1189, 1271, 1323
TspGWI	ACGGA	1 cut(s)	147
TspRI	CASTG	9 cut(s)	495, 595, 1045, 1114, 1474, 1587, 1723, 2173, 2277
VpaK11BI	GGWCC	2 cut(s)	134, 1801
XapI	RAATTY	4 cut(s)	32, 444, 673, 902
XceI	RCATGY	1 cut(s)	1450
XmaJI	CCTAGG	1 cut(s)	1757
XmnI	GAANNNNTTC	2 cut(s)	699, 954
XspI	CTAG	5 cut(s)	438, 992, 1758, 1832, 1944
ZraI	GACGTC	1 cut(s)	1474
ZrmI	AGTACT	2 cut(s)	370, 2066

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.