pycom15g05980

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0003946
Physical Location & Seq
Reverse (-)
3636036 .. 3642088
6053 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 5271 bp
ATGGTAAACGACGTAAATGAAGCTTTACGTTCCGCTAAACTTCACATACAAGAGATTAGGACCAAAAAGTTCTCAATAGCGAAGAAAGAAGCAAACCCTTTAACTCTCGATCTTCACCATGCCGTCACTAGTCTCTCTGCGGAGCTTTACCAGAAAGACATCCATTTTCTCATGGAGCTCATACAGAATGCAGAAGACAATGAATACAAGGAAGGAGTTGAACCAACGTTGGAATTTGTGCTGACAAAGAAAGACATAACTGGGAGTGGAGCACCAGCTACTCTGCTTGTTTTTAATAACGAGGTTGGCTTTTCCAGGAAGAATATGGACTCGATTTGCAGCATAGGCCGTTCCACAAAGAAGGGAAAGAGACGGCAGGGATTTATTGGAGAAAAAGGTATTGGATTTAAAAGTGTATTCCTTGTGAGTTCACGGCCTCATATATTTAGCAACGGATATCAGGTCAGTTTTATGGAAGAACCAAATCAAGATTGTGGTATTGGTTATATAGTTCCTGAATGGGTTACTGGAAAGCCATATTTATCAAGCATATGCGAGGTGTATGGTTTCAACAAAATCTTGCCAACAACTACGTTTATTCTTCCTCTAAAGCCTGACAAGGTGGAAGCTGTCAGAATGCAGCTGTCAGAGCTGCACCCAGAGCTTCTCCTGTTCTTATCCAAGATAAAGCACGTATACGTACGTGGATGTGATCCACAAGAAGCTGATGATGTCTCCACAATCTCTATATTCAGTGAGACTGAGCATGTGAACTTGAGTGATAAAGGAGCCAATTCACGTGTTGTTCAACTTTCTGTGAAAGAAAAAAAATGTGATACTGACGAGTTGTGCAAGTATTATTTGTGGAGAGAGTCATTTCCAGTAAAACCGGGCAATAGAGTTAATACAAGGATGGATGTGGAGGAATGGGTCATCACCCTTGCTTTCCCATTTGGAGAAAGACTGAGAAGGGGAACGTCGTCTGTTGGTGTATTTTCTTTCCTGCCTACTGCAATGGTCACCAACCTCCCCTTTGTAATTCAAGCTGATTTCATTCTTGCTTCTTCACGAGAATCTATTCTGTTGGATGATGTCTGGAACTTGGGAATACTTGAATGTGTGCCATCCGCCTTCATGAATGCCTTCCAGTCATGTGTGAGAGAGCTCTCCCTCTTCCCTTCACCTGGTCAGGCATTTGAGTTCTTACCTGCTCAAGCTTCTCCGATTCCCACATTTGACAACTTAAGGGAGTCTATTAGAGTTAAATTGGAAGGTTTACAGATAGTGCCTTGTCAAATGCTTTCTGGCGGGAATTGGTTGTTCCTGCAGCCTAAACATGCAGTTCGGATCTTGCCAAAATTTAGGGCCCTAGTGCTACAAATGAAAAGTGAAGGGGCTTTTTCAAGTGGTTCACCTTTATTGAAGAGGGTCTTGCATTCGTCACTGGACCTTGAAAAATACAGTGCAGTTCTGTACTTTCTAGGTGTGGTGTGGGCTAGTGGTCATTGGTACACAGAATGCATCAGATCCTGCAATCTTCTGATGGTATCTGATGATGTCTACATTGATCTACTTGATTTTATTGCTGATAATGAGGTAATGACGTCAAAGCATATTTCAGTCATACCTCTTATTAAATACATCAATCGGGAAGGCAATTTGCAATTGTGTACAATTACCAAAACCGCGAAGGCGGAGTCCAAGATTCAATATGCTGAGAAGAAGGAAGTTCATACATGGCTGAACAAATGCAATATGGAGTTTGGATGTCCTGATAATATGTATTTCTTGCCAGATAACGTACAAAAAGCTCTTTTAGATAATCAGAGAAGTGTTGCCAGAAATAAAAAATCAACGCACGGGATAAAATCATCTGTCTGCAATTGGCTTTGCTACTATGCAGGAGTGAAATCTTGTACAGCATATGATTATGCTGTATTTCTTTGCAATCATGTAAACAAGGAGCCAGGTCTTGCACTTACACTTGCTAATTTCCTTTATCATGCGCACAAGAAATCCTTCCTTAGTGATTATGAGACTTCTTATCTTTGTGGAAGTTTCCCGCTTATTGATGGAACTGACCGTGTCAGAATGCAGAGAACTGTTACCCTTGTCCCTGCATCAGGTAGCAAATGGGTGAAGTTGTTTGGTTCCCAGAATCCATTTGTTGAACAGAACTATGTTGATATAGGGAATGCTTATGCAAAAAGTAGTATGTTTTTGGGAGACTCGGTGCTTGAGAAGGAGCTTCTTAATTTTATTGAAAGGCACAGTAAAGCTATGGACTTACCCGAGTTGTCTCCTCCAGATGTGGTTTTAGAGAAAGCATCTCATGAACTATCTAGTGAGCAGGCCTTTTTGCTGCTTGATTGGATCAGATTACTCCGGACTAAGGGTTCATCTCTTCCGACAAAGTTCATTGAAAGCATTCGAGGTGGTAAGTGGATGAAGACATACTCAGGTTATAGTTCTCCAAGAAAGGCCATTCTACCAGATGAAGCAGGTAAATCTATTCTTGATATGATGAAGCATGTTCTGAAGGATGTCTGTATTATAGACCTCGAATTTTACATGAATCGGATCAATCTTTATCAAGATGAATTGAAATTTCTTGGTGTCGGGCATGGATCAGATGAAATGAGGAAGCTGGTTACTAATTGCTTAAAGTCTCTTGCTTCTCCTCGAATGAGCAAAGACTGCGCATTTTCTTTGTTGAATTTCATTAGTTTCTGTAGAGGGCGGAATGTGATTGACAAGGATTGGTTGACTGTTATGAAGGAGAAGAAGTGGCTGAAGACTCATCAGGGTTATAGTGCTCCAAAGGGATCTATGCTTTTGCCGTCTGAGATAGAAGTGGAAGCCTGTTTGAAAATCACAAATCTTCCAATTGTTGATGAAACATTCTATGGAAGCAGATTAGGTTCTTTCATATCTGAGTTAACGCTACTTGGAGTTGCATGTGGTCTTGAGGAGGTGCAGAAATCGATTGCAGAGAATATGAATTTAACATCAAATCTGTCATCCATGACTGGTAGTTGTGGTTTATTGATTCTTAAGTGTATCAGATGCTTGGGGTCTGGGGCTGCAGGCTTAATTAACAGGATAAAATGTCAACCTTGGATGAAGACAAATTTGGGTTTTAAAAGACCTTCAGAAACGGCTCTTCCTGGTTCCCAATGGGGTTCTTTGTTCACTGCTCTCCAGGTTCCTGCCATAGATGCACTGTATTATGGGGATGCAATTACACATTTTGTTGAGGAGTTGAATGCCTTGGATGTTGTAGTTGATGATTCTGGAGCATCTAAGATGATCGCTGTCCAATTTAATTCCCTAGTGTCTTCTTCTAACCTAGCTCCTTCAACAGTGATGTCACTGCTAGGTTGCATCCGGGAACTGAGTCAAACTATGTCTTTATCGTCCCCTGAACTGATGTGGTTGACATCTGAGAAGTGGTTAAAAACACGACATGGTTACAAGACTCCTAGAGAATCAATTCTTTTTAGCTCCAAGTGGGGGTCAATCTCGTTCTTTGTTGATCTTCCTCTAATTGATGATGCGTATTATGGTATCGACATATATAAATTCAGGGGTGAGCTGCAGATATTGGGTGTAATCATGGATTTTGAACAAGGAGCATCATTTGTTGCTAAGGGTGTCCGCAGCCCTATTGAAGGGGAACTACTTAGTGCAGATGGTATGATGTCAATTCTAGAGTGCATTAAGCATCTGATGTCAAGTTCTCTTGATGAGGCTTTAGTGGGTGACTTGGTCAGTAATGTCGCAAAAGGCACGTGTTTGAAGACCCTGAGTGGTTACAAAACTCCAGAAGAATGTGTTCTTTTTGATCCAACATGGGATTGTATTCTAAAGAGGTCAGATGTGCCAAGCATTGATGAATTTTATTTTGGAACTAACATTCATGCGTACAAGAATCAGTTGAGAGATATTGGCGTGAAGGTAGATCCTTTGGATGTTTGTTCTTTTCTTTCTGCATTTCTTTTGTCCCAGGCAAAAACGGCTGTAATAAGAAGGATTTATAGCTTCCTTAACAAGTTCAAGTGGAGTCCAACAGTTCAAGATAAATGCAACTCTCAGGTGTGGATACCTAATCCCGAGGGCACAGGTGTGTGGGTTAATTCTCAAGATTGTGTACTTCATGATCGGAAAAATCTGTTCGGCTCCAGCCTGTTTTGTCTTGACAAGTTTTACAAGAAAGAGCTCCTCCCCGTGTTTTCTTCGGCTTTTGGAGTTTCCGATAATCCTTCCATCAATAACTACGTGCAGCTATGGAACAATTGGGCCTTGAGGGATAAGTGTCAAGTAACTGTCAGAGAATGCAGCTTGTTTTGCGAATATATTGTAGACAACTGGAATCAAGACGTAGAAGAAAATCTGAAGCTGAACTTGATCAGATTACCTGCCACAATGTCCACAGTTGAAGAAATATATCTGATGAGTAGAGAGGAAGTGTTCATTGCTGATGACTTGCAACTGAAAAAGATTTTCTCGAGCTTCGACAAGGCTCCTTTGTTTGTGTGGATTCCCAACAGTTTCAGCGAGTTTCTTATTTCTCCTAGAAGGCTGCATCAAATTTATGAATTACTTGGAGTTAGAAAGGTTTCTGACTCAGTTGAGTGCAATGTCAGTAGCATGCTATCGGTGGATCATTGTGAAAAGGTTGATGCAAGAAACGGATTGATAGGAAGGGGTTTAATCAAAATTATTCTTGGTTTTCTTGCCGGTCCTGAGGTAAACATGTCAATGAAAGAGAGACACAACGCTGCTAAATCAATGGTTATGCTGTCGGTGTACAAGAGTGACAAACCAATTCAAGTCTGTTACAGACTAGTTCCATCTGCAAGTACATCAGTGGAAGTTGAAAAGTTAAAGCTGGTTCTTTGGGAGAAGGATTCTCAGCGGTTGCTTATGGATAAATCTGGCTATGAAAATGGGAAAGATGATCTTGAATTTGTTACGTCTTTTGCTGACGAACTCGCACAAGGATTGTTAGCACAGGCAAAACCCACTGCAGCAGATACATTGAGTAAGATTATCCAAATTGGGTTCATGTTTCATTTCAATGAGAATGAAGTTGACTTTTTGCTGATGAAGCAAAATTTGGAGCTGTTAGTGGAGGATGTGGCGTTTCTCGACAGTGCATTCCTCTCTTCAGAAGGACCTTCTCGTGTTTACCGAAAGCGTGCAAGCAAGAAGTTGGAGCTTGTAGCCCCTTCCACACCAATGCTTTCCGGCAAGAAACGACGTCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1757

Amino Acids

197.56

Weight (kDa)

6.71

Isoelectric Point (pI)

39.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SACS PF25794 50 - 155 4.1e-10 Sacsin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 1607, 5266
Acc16I TGCGCA 2 cut(s) 2007, 2701
Acc36I ACCTGC 3 cut(s) 1216, 2492, 4426
AccI GTMKAC 3 cut(s) 696, 1560, 4362
AccII CGCG 1 cut(s) 1688
AccIII TCCGGA 1 cut(s) 2385
AclI AACGTT 1 cut(s) 227
AcuI CTGAAG 5 cut(s) 2558, 2813, 3135, 4415, 5154
AcvI CACGTG 2 cut(s) 800, 3792
AcyI GRCGYC 2 cut(s) 1604, 5263
AfiI CCNNNNNNNGG 5 cut(s) 1184, 2123, 2392, 3513, 3671
AflII CTTAAG 2 cut(s) 1243, 3053
AflIII ACRYGT 3 cut(s) 799, 3791, 4755
AhlI ACTAGT 2 cut(s) 128, 4846
AjnI CCWGG 6 cut(s) 314, 1183, 1966, 3166, 3201, 4005
AjuI GAANNNNNNNTTGG 8 cut(s) 384, 416, 3116, 3148, 3888, 3920, 5102, 5134
AleI CACNNNNGTG 1 cut(s) 4124
AloI GAACNNNNNNTCC 2 cut(s) 2380, 2412
Alw21I GWGCWC 5 cut(s) 180, 274, 1167, 2818, 4221
Alw26I GTCTC 9 cut(s) 137, 364, 739, 752, 2030, 2220, 2304, 2673, 4765
AlwNI CAGNNNCTG 1 cut(s) 1530
Ama87I CYCGRG 3 cut(s) 2291, 4112, 4507
Aor13HI TCCGGA 1 cut(s) 2385
AoxI GGCC 6 cut(s) 346, 434, 1365, 2352, 2481, 4299
ApaI GGGCCC 1 cut(s) 1369
ArsI GACNNNNNNTTYG 2 cut(s) 1682, 1714
Asp700I GAANNNNTTC 7 cut(s) 1077, 1142, 2018, 2427, 3492, 3834, 4618
AspLEI GCGC 2 cut(s) 2008, 2702
AspS9I GGNCC 7 cut(s) 60, 1365, 1366, 1447, 4299, 4742, 5177
AsuC2I CCSGG 2 cut(s) 891, 3389
AsuHPI GGTGA 8 cut(s) 107, 928, 1012, 1173, 1404, 2149, 3602, 3773
AvaI CYCGRG 3 cut(s) 2291, 4112, 4507
AvaII GGWCC 4 cut(s) 60, 1447, 4742, 5177
AxyI CCTNAGG 1 cut(s) 4746
BaeGI GKGCMC 2 cut(s) 1369, 4121
BanII GRGCYC 4 cut(s) 180, 1167, 1369, 4221
BarI GAAGNNNNNNTAC 2 cut(s) 3663, 3695
BauI CACGAG 2 cut(s) 1068, 5184
BbrPI CACGTG 2 cut(s) 800, 3792
BbsI GAAGAC 6 cut(s) 201, 2456, 2801, 3131, 3330, 3806
Bbv12I GWGCWC 5 cut(s) 180, 274, 1167, 2818, 4221
BccI CCATC 7 cut(s) 907, 1132, 1537, 2066, 3686, 4274, 4861
BceAI ACGGC 7 cut(s) 107, 333, 389, 449, 2824, 3174, 4032
BciT130I CCWGG 6 cut(s) 316, 1185, 1968, 3168, 3203, 4007
BciVI GTATCC 1 cut(s) 4095
BclI TGATCA 1 cut(s) 4407
BcnI CCSGG 2 cut(s) 891, 3389
BcoDI GTCTC 9 cut(s) 137, 364, 739, 752, 2030, 2220, 2304, 2673, 4765
BcuI ACTAGT 2 cut(s) 128, 4846
BfmI CTRYAG 5 cut(s) 1325, 2731, 3084, 3596, 5028
BfrI CTTAAG 2 cut(s) 1243, 3053
BfuAI ACCTGC 3 cut(s) 1216, 2492, 4426
BfuI GTATCC 1 cut(s) 4095
Bme1390I CCNGG 8 cut(s) 316, 891, 1185, 1968, 3168, 3203, 3389, 4007
Bme18I GGWCC 4 cut(s) 60, 1447, 4742, 5177
BmeT110I CYCGRG 3 cut(s) 2291, 4112, 4507
BmgT120I GGNCC 7 cut(s) 60, 1365, 1366, 1447, 4299, 4742, 5177
BmiI GGNNCC 8 cut(s) 790, 1367, 1965, 2152, 3172, 3207, 4180, 4524
BmrFI CCNGG 8 cut(s) 316, 891, 1185, 1968, 3168, 3203, 3389, 4007
BmrI ACTGGG 1 cut(s) 270
BmuI ACTGGG 1 cut(s) 270
BpiI GAAGAC 6 cut(s) 201, 2456, 2801, 3131, 3330, 3806
BpmI CTGGAG 5 cut(s) 2289, 3185, 3315, 3807, 4165
Bpu10I CCTNAGC 1 cut(s) 3648
BpuEI CTTGAG 6 cut(s) 796, 1197, 2258, 2987, 4125, 4324
BpuMI CCSGG 2 cut(s) 891, 3389
Bsa29I ATCGAT 1 cut(s) 2984
BsaAI YACGTR 6 cut(s) 694, 700, 704, 800, 3792, 4279
BsaHI GRCGYC 2 cut(s) 1604, 5263
BsaJI CCNNGG 4 cut(s) 3116, 3270, 4005, 4113
BsaWI WCCGGW 1 cut(s) 2385
BsaXI ACNNNNNCTCC 4 cut(s) 2217, 2247, 2771, 2801
Bsc4I CCNNNNNNNGG 5 cut(s) 1184, 2123, 2392, 3513, 3671
Bse118I RCCGGY 1 cut(s) 4739
Bse1I ACTGG 7 cut(s) 265, 532, 881, 1147, 1449, 3034, 4373
Bse21I CCTNAGG 1 cut(s) 4746
Bse3DI GCAATG 3 cut(s) 1020, 4473, 4645
BseAI TCCGGA 1 cut(s) 2385
BseBI CCWGG 6 cut(s) 316, 1185, 1968, 3168, 3203, 4007
BseCI ATCGAT 1 cut(s) 2984
BseDI CCNNGG 4 cut(s) 3116, 3270, 4005, 4113
BseLI CCNNNNNNNGG 5 cut(s) 1184, 2123, 2392, 3513, 3671
BseMI GCAATG 3 cut(s) 1020, 4473, 4645
BseNI ACTGG 7 cut(s) 265, 532, 881, 1147, 1449, 3034, 4373
BseRI GAGGAG 5 cut(s) 2292, 2670, 2984, 3272, 4211
BseSI GKGCMC 2 cut(s) 1369, 4121
BsgI GTGCAG 5 cut(s) 638, 1485, 2996, 3708, 4301
Bsh1236I CGCG 1 cut(s) 1688
BshFI GGCC 6 cut(s) 348, 436, 1367, 2354, 2483, 4301
BshVI ATCGAT 1 cut(s) 2984
BsiHKAI GWGCWC 5 cut(s) 180, 274, 1167, 2818, 4221
BsiHKCI CYCGRG 3 cut(s) 2291, 4112, 4507
BsiSI CCGG 5 cut(s) 890, 2386, 3388, 4740, 5250
BsiWI CGTACG 1 cut(s) 700
BslFI GGGAC 3 cut(s) 2099, 3403, 3988
BslI CCNNNNNNNGG 5 cut(s) 1184, 2123, 2392, 3513, 3671
BsmAI GTCTC 9 cut(s) 137, 364, 739, 752, 2030, 2220, 2304, 2673, 4765
BsmBI CGTCTC 1 cut(s) 364
BsmFI GGGAC 3 cut(s) 2099, 3403, 3988
BsnI GGCC 6 cut(s) 348, 436, 1367, 2354, 2483, 4301
BsoBI CYCGRG 3 cut(s) 2291, 4112, 4507
Bsp120I GGGCCC 1 cut(s) 1365
Bsp1286I GDGCHC 7 cut(s) 180, 274, 1167, 1369, 2818, 4121, 4221
Bsp13I TCCGGA 1 cut(s) 2385
Bsp1407I TGTACA 3 cut(s) 1670, 1916, 4809
BspANI GGCC 6 cut(s) 348, 436, 1367, 2354, 2483, 4301
BspDI ATCGAT 1 cut(s) 2984
BspEI TCCGGA 1 cut(s) 2385
BspFNI CGCG 1 cut(s) 1688
BspHI TCATGA 3 cut(s) 1134, 2332, 4156
BspLI GGNNCC 8 cut(s) 790, 1367, 1965, 2152, 3172, 3207, 4180, 4524
BspMAI CTGCAG 4 cut(s) 1329, 3088, 3600, 5032
BspMI ACCTGC 3 cut(s) 1216, 2492, 4426
BspQI GCTCTTC 1 cut(s) 3168
BspTI CTTAAG 2 cut(s) 1243, 3053
BsrDI GCAATG 3 cut(s) 1020, 4473, 4645
BsrFI RCCGGY 1 cut(s) 4739
BsrGI TGTACA 3 cut(s) 1670, 1916, 4809
BsrI ACTGG 7 cut(s) 265, 532, 881, 1147, 1449, 3034, 4373
BssAI RCCGGY 1 cut(s) 4739
BssECI CCNNGG 4 cut(s) 3116, 3270, 4005, 4113
BssNAI GTATAC 1 cut(s) 697
BssNI GRCGYC 2 cut(s) 1604, 5263
BssSI CACGAG 2 cut(s) 1068, 5184
BssT1I CCWWGG 2 cut(s) 3116, 3270
Bst1107I GTATAC 1 cut(s) 697
Bst2BI CACGAG 2 cut(s) 1068, 5184
Bst2UI CCWGG 6 cut(s) 316, 1185, 1968, 3168, 3203, 4007
Bst6I CTCTTC 5 cut(s) 1178, 1418, 2409, 3168, 5173
BstACI GRCGYC 2 cut(s) 1604, 5263
BstAFI CTTAAG 2 cut(s) 1243, 3053
BstAPI GCANNNNNTGC 1 cut(s) 2697
BstAUI TGTACA 3 cut(s) 1670, 1916, 4809
BstBAI YACGTR 6 cut(s) 694, 700, 704, 800, 3792, 4279
BstC8I GCNNGC 5 cut(s) 2352, 3088, 4652, 5202, 5206
BstEII GGTNACC 1 cut(s) 1018
BstENI CCTNNNNNAGG 2 cut(s) 2121, 3669
BstFNI CGCG 1 cut(s) 1688
BstHHI GCGC 2 cut(s) 2008, 2702
BstMAI GTCTC 9 cut(s) 137, 364, 739, 752, 2030, 2220, 2304, 2673, 4765
BstMWI GCNNNNNNNGC 8 cut(s) 345, 649, 661, 2697, 3218, 4016, 4347, 5110
BstNI CCWGG 6 cut(s) 316, 1185, 1968, 3168, 3203, 4007
BstNSI RCATGY 6 cut(s) 770, 1340, 2534, 2961, 4654, 4759
BstPI GGTNACC 1 cut(s) 1018
BstSCI CCNGG 8 cut(s) 314, 889, 1183, 1966, 3166, 3201, 3387, 4005
BstSFI CTRYAG 5 cut(s) 1325, 2731, 3084, 3596, 5028
BstSLI GKGCMC 2 cut(s) 1369, 4121
BstSNI TACGTA 1 cut(s) 700
BstUI CGCG 1 cut(s) 1688
BstV2I GAAGAC 6 cut(s) 201, 2456, 2801, 3131, 3330, 3806
BstX2I RGATCY 4 cut(s) 1347, 1526, 2825, 3961
BstYI RGATCY 4 cut(s) 1347, 1526, 2825, 3961
BstZ17I GTATAC 1 cut(s) 697
Bsu15I ATCGAT 1 cut(s) 2984
Bsu36I CCTNAGG 1 cut(s) 4746
BsuI GTATCC 1 cut(s) 4095
BsuRI GGCC 6 cut(s) 348, 436, 1367, 2354, 2483, 4301
BsuTUI ATCGAT 1 cut(s) 2984
BtsI GCAGTG 3 cut(s) 3192, 3371, 5025
BveI ACCTGC 3 cut(s) 1216, 2492, 4426
Cac8I GCNNGC 5 cut(s) 2352, 3088, 4652, 5202, 5206
CaiI CAGNNNCTG 1 cut(s) 1530
CciI TCATGA 3 cut(s) 1134, 2332, 4156
CfoI GCGC 2 cut(s) 2008, 2702
Cfr10I RCCGGY 1 cut(s) 4739
Cfr13I GGNCC 7 cut(s) 60, 1365, 1366, 1447, 4299, 4742, 5177
ClaI ATCGAT 1 cut(s) 2984
CsiI ACCWGGT 1 cut(s) 1183
DraI TTTAAA 2 cut(s) 409, 3142
Eam1104I CTCTTC 5 cut(s) 1178, 1418, 2409, 3168, 5173
EarI CTCTTC 5 cut(s) 1178, 1418, 2409, 3168, 5173
EciI GGCGGA 3 cut(s) 1117, 1709, 2755
Ecl136II GAGCTC 3 cut(s) 178, 1165, 4219
Eco105I TACGTA 1 cut(s) 700
Eco130I CCWWGG 2 cut(s) 3116, 3270
Eco147I AGGCCT 1 cut(s) 2354
Eco24I GRGCYC 4 cut(s) 180, 1167, 1369, 4221
Eco32I GATATC 1 cut(s) 458
Eco47I GGWCC 4 cut(s) 60, 1447, 4742, 5177
Eco53kI GAGCTC 3 cut(s) 178, 1165, 4219
Eco57I CTGAAG 5 cut(s) 2558, 2813, 3135, 4415, 5154
Eco72I CACGTG 2 cut(s) 800, 3792
Eco81I CCTNAGG 1 cut(s) 4746
Eco88I CYCGRG 3 cut(s) 2291, 4112, 4507
Eco91I GGTNACC 1 cut(s) 1018
EcoICRI GAGCTC 3 cut(s) 178, 1165, 4219
EcoNI CCTNNNNNAGG 2 cut(s) 2121, 3669
EcoO109I RGGNCCY 3 cut(s) 1365, 1366, 5177
EcoO65I GGTNACC 1 cut(s) 1018
EcoRII CCWGG 6 cut(s) 314, 1183, 1966, 3166, 3201, 4005
EcoRV GATATC 1 cut(s) 458
EcoT14I CCWWGG 2 cut(s) 3116, 3270
EcoT22I ATGCAT 1 cut(s) 1523
EcoT38I GRGCYC 4 cut(s) 180, 1167, 1369, 4221
ErhI CCWWGG 2 cut(s) 3116, 3270
Esp3I CGTCTC 1 cut(s) 364
FalI AAGNNNNNCTT 8 cut(s) 1415, 1447, 2003, 2035, 4388, 4420, 4947, 4979
FaqI GGGAC 3 cut(s) 2099, 3403, 3988
FauI CCCGC 2 cut(s) 1301, 2070
FauNDI CATATG 2 cut(s) 551, 1924
FbaI TGATCA 1 cut(s) 4407
FblI GTMKAC 3 cut(s) 696, 1560, 4362
FriOI GRGCYC 4 cut(s) 180, 1167, 1369, 4221
FspAI RTGCGCAY 1 cut(s) 2007
FspI TGCGCA 2 cut(s) 2007, 2701
GlaI GCGC 2 cut(s) 2007, 2701
GsuI CTGGAG 5 cut(s) 2289, 3185, 3315, 3807, 4165
HaeIII GGCC 6 cut(s) 348, 436, 1367, 2354, 2483, 4301
HapII CCGG 5 cut(s) 890, 2386, 3388, 4740, 5250
HhaI GCGC 2 cut(s) 2008, 2702
Hin1I GRCGYC 2 cut(s) 1604, 5263
Hin6I GCGC 2 cut(s) 2006, 2700
HinP1I GCGC 2 cut(s) 2006, 2700
HincII GTYRAC 5 cut(s) 2766, 2940, 3113, 3438, 5095
HindII GTYRAC 5 cut(s) 2766, 2940, 3113, 3438, 5095
HindIII AAGCTT 2 cut(s) 21, 1215
HpaI GTTAAC 1 cut(s) 2940
HpaII CCGG 5 cut(s) 890, 2386, 3388, 4740, 5250
HphI GGTGA 8 cut(s) 107, 928, 1012, 1173, 1404, 2149, 3602, 3773
Hpy99I CGWCG 3 cut(s) 14, 982, 5265
HpyF10VI GCNNNNNNNGC 8 cut(s) 345, 649, 661, 2697, 3218, 4016, 4347, 5110
Hsp92I GRCGYC 2 cut(s) 1604, 5263
HspAI GCGC 2 cut(s) 2006, 2700
Kpn2I TCCGGA 1 cut(s) 2385
Ksp22I TGATCA 1 cut(s) 4407
KspAI GTTAAC 1 cut(s) 2940
LguI GCTCTTC 1 cut(s) 3168
MabI ACCWGGT 1 cut(s) 1183
MfeI CAATTG 4 cut(s) 1664, 1882, 2886, 4294
MflI RGATCY 4 cut(s) 1347, 1526, 2825, 3961
MhlI GDGCHC 7 cut(s) 180, 274, 1167, 1369, 2818, 4121, 4221
MmeI TCCRAC 6 cut(s) 210, 1065, 2432, 3872, 4091, 5196
Mph1103I ATGCAT 1 cut(s) 1523
MroI TCCGGA 1 cut(s) 2385
MroXI GAANNNNTTC 7 cut(s) 1077, 1142, 2018, 2427, 3492, 3834, 4618
MslI CAYNNNNRTG 4 cut(s) 4124, 4760, 5079, 5240
MspA1I CMGCKG 2 cut(s) 643, 4918
MspCI CTTAAG 2 cut(s) 1243, 3053
MspI CCGG 5 cut(s) 890, 2386, 3388, 4740, 5250
MspR9I CCNGG 8 cut(s) 316, 891, 1185, 1968, 3168, 3203, 3389, 4007
MunI CAATTG 4 cut(s) 1664, 1882, 2886, 4294
MvaI CCWGG 6 cut(s) 316, 1185, 1968, 3168, 3203, 4007
MvnI CGCG 1 cut(s) 1688
MwoI GCNNNNNNNGC 8 cut(s) 345, 649, 661, 2697, 3218, 4016, 4347, 5110
NciI CCSGG 2 cut(s) 891, 3389
NdeI CATATG 2 cut(s) 551, 1924
NlaIV GGNNCC 8 cut(s) 790, 1367, 1965, 2152, 3172, 3207, 4180, 4524
NmuCI GTSAC 6 cut(s) 124, 1018, 1440, 3369, 3761, 4817
NsbI TGCGCA 2 cut(s) 2007, 2701
NsiI ATGCAT 1 cut(s) 1523
NspI RCATGY 6 cut(s) 770, 1340, 2534, 2961, 4654, 4759
OliI CACNNNNGTG 1 cut(s) 4124
PacI TTAATTAA 1 cut(s) 3096
PaeI GCATGC 1 cut(s) 4654
PaeR7I CTCGAG 1 cut(s) 4507
PagI TCATGA 3 cut(s) 1134, 2332, 4156
PceI AGGCCT 1 cut(s) 2354
PciI ACATGT 1 cut(s) 4755
PciSI GCTCTTC 1 cut(s) 3168
PcsI WCGNNNNNNNCGW 1 cut(s) 5191
PdmI GAANNNNTTC 7 cut(s) 1077, 1142, 2018, 2427, 3492, 3834, 4618
Pfl23II CGTACG 1 cut(s) 700
PflFI GACNNNGTC 2 cut(s) 2084, 3767
PfoI TCCNGGA 2 cut(s) 314, 3387
PmaCI CACGTG 2 cut(s) 800, 3792
PmlI CACGTG 2 cut(s) 800, 3792
Ppu21I YACGTR 6 cut(s) 694, 700, 704, 800, 3792, 4279
PpuMI RGGWCCY 1 cut(s) 5177
PscI ACATGT 1 cut(s) 4755
Psp124BI GAGCTC 3 cut(s) 180, 1167, 4221
Psp1406I AACGTT 1 cut(s) 227
Psp5II RGGWCCY 1 cut(s) 5177
Psp6I CCWGG 6 cut(s) 314, 1183, 1966, 3166, 3201, 4005
PspCI CACGTG 2 cut(s) 800, 3792
PspEI GGTNACC 1 cut(s) 1018
PspGI CCWGG 6 cut(s) 314, 1183, 1966, 3166, 3201, 4005
PspLI CGTACG 1 cut(s) 700
PspN4I GGNNCC 8 cut(s) 790, 1367, 1965, 2152, 3172, 3207, 4180, 4524
PspOMI GGGCCC 1 cut(s) 1365
PspPI GGNCC 7 cut(s) 60, 1365, 1366, 1447, 4299, 4742, 5177
PspPPI RGGWCCY 1 cut(s) 5177
PstI CTGCAG 4 cut(s) 1329, 3088, 3600, 5032
PstNI CAGNNNCTG 1 cut(s) 1530
PsuI RGATCY 4 cut(s) 1347, 1526, 2825, 3961
PsyI GACNNNGTC 2 cut(s) 2084, 3767
PvuII CAGCTG 1 cut(s) 643
RseI CAYNNNNRTG 4 cut(s) 4124, 4760, 5079, 5240
SacI GAGCTC 3 cut(s) 180, 1167, 4221
SapI GCTCTTC 1 cut(s) 3168
Sau96I GGNCC 7 cut(s) 60, 1365, 1366, 1447, 4299, 4742, 5177
ScrFI CCNGG 8 cut(s) 316, 891, 1185, 1968, 3168, 3203, 3389, 4007
SduI GDGCHC 7 cut(s) 180, 274, 1167, 1369, 2818, 4121, 4221
SexAI ACCWGGT 1 cut(s) 1183
SfcI CTRYAG 5 cut(s) 1325, 2731, 3084, 3596, 5028
Sfr274I CTCGAG 1 cut(s) 4507
SinI GGWCC 4 cut(s) 60, 1447, 4742, 5177
SlaI CTCGAG 1 cut(s) 4507
SmiMI CAYNNNNRTG 4 cut(s) 4124, 4760, 5079, 5240
SmlI CTYRAG 9 cut(s) 775, 1212, 1243, 2237, 2966, 3053, 4140, 4303, 4507
SmoI CTYRAG 9 cut(s) 775, 1212, 1243, 2237, 2966, 3053, 4140, 4303, 4507
SnaBI TACGTA 1 cut(s) 700
SpeI ACTAGT 2 cut(s) 128, 4846
SphI GCATGC 1 cut(s) 4654
SseBI AGGCCT 1 cut(s) 2354
SstI GAGCTC 3 cut(s) 180, 1167, 4221
StuI AGGCCT 1 cut(s) 2354
StyD4I CCNGG 8 cut(s) 314, 889, 1183, 1966, 3166, 3201, 3387, 4005
StyI CCWWGG 2 cut(s) 3116, 3270
TatI WGTACW 6 cut(s) 1473, 1670, 1916, 4150, 4809, 4862
TseFI GTSAC 6 cut(s) 124, 1018, 1440, 3369, 3761, 4817
Tsp45I GTSAC 6 cut(s) 124, 1018, 1440, 3369, 3761, 4817
TspGWI ACGGA 2 cut(s) 468, 4707
Tth111I GACNNNGTC 2 cut(s) 2084, 3767
Vha464I CTTAAG 2 cut(s) 1243, 3053
VpaK11BI GGWCC 4 cut(s) 60, 1447, 4742, 5177
XagI CCTNNNNNAGG 2 cut(s) 2121, 3669
XbaI TCTAGA 1 cut(s) 3709
XceI RCATGY 6 cut(s) 770, 1340, 2534, 2961, 4654, 4759
XcmI CCANNNNNNNNNTGG 1 cut(s) 322
XhoI CTCGAG 1 cut(s) 4507
XmiI GTMKAC 3 cut(s) 696, 1560, 4362
XmnI GAANNNNTTC 7 cut(s) 1077, 1142, 2018, 2427, 3492, 3834, 4618
ZraI GACGTC 2 cut(s) 1605, 5264
Zsp2I ATGCAT 1 cut(s) 1523
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.