Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

OG0002222

Physical Location & Seq

Genomic Coordinates Forward (+)

11601362 .. 11606250

Gene Structure 4889 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3174 bp

ATGACCGCTCCTCCTCGCGCCGCTACTTCATCACTTTCTCTTCTCTTCGTCGTCATCGCCGTTTCCATTTCCATCTCCAGCTCTCTCCTCCGCTTCAAACCCCAAAATTCCCCAACCCTAATCGTAAACCTTCCCCCAAATTTGTCGCGTTCCGAGACAAACCGCCAAAACTACGTCGTTCGATTCGTGGAATACAGGAGGGCGGAGGAGCTCCGAGAGTATTTGAAATCGAGTGTGCGATCCGATGGGTGGGATTGGATCGAGAGGAACAACCCGGCGAAGAAGTACCCGACGGATTTCGGTTTGGTTTGGATTGAAGAATCGTCGAGAGAGACGGTGGTTTCAGAAATTTGGAGCTTGGGAATGGTGAAGGATGTGAATGCGGATGTGAGGTATGGGAGGGGTTTGCTTGCTGAGGGAAAGAGGGACAAAGTTGGGGCTTTTGTCGATGGGGAGAAGCGGCCGGGTAAGATTTTGACGGCGATGTCGTTCAGTGAAGCCGACGGGGATGCATTTTACTCGGCCGCAAGTAATTATTCGGTTAGCTGGAGAAGGCGGCTCATGTCTCAGAGATCTCAAGTTACTTCAATGTTTGGGGCGGAGTCCCTTTGGGAAAAAGGATATGCTGGTGCAAAAGTTAAAATGGCCATCTTTGACACTGGAATTCGAGCAGATCACCCGCATTTTCGTAATATCAAGGAGCGCACAAACTGGACAAATGAAGATACTTTGAATGACAACCTTGGGCATGGAACATTTGTTGCTGGTGTTATTGCTGGTGTAGATGCTGAGTGTCTCGGTTTTGCTCCTGACACAGAGATTTATGCTTTTCGTGTGTTTACAGATGCACAGGTATCATACACATCATGGTTCCTTGACGCATTCAACTATGCAATTGCGACCAATATGGATGTGCTAAATCTCAGTATAGGTGGACCTGATTACTTGGACCTCCCATTTGTAGAGAAGGTTTGGGAATTAACGGCAAACAACATAATTATGGTTTCGGCAATTGGAAATGACGGACCGCTATACGGAACTCTGAACAATCCAGCAGACCAAAGTGATGTCATTGGCGTTGGTGGCATTGACTACAGTGATCACATTGCTTCTTTCTCGTCACGTGGCATGAGTACATGGGAGATGCCTCATGGTTATGGTCGGGTGAAGCCAGATGTCGTTGCATACGGACGGGACATCATGGGATCCAAGATCAGTACAGGCTGTAAAAGCTTATCGGGCACAAGTGTGGCAAGTCCTGTGGTTGCTGGTATGGTATGTCTTCTTGTCAGTGTTATCCCTGTGAGCAATAGGAAGGATATTTTAAATCCAGCAAGCATGAAACAAGCACTGGTTGAGGGTGCTGCAAAGCTTTCTGGTCCGAATATGTATGAGCAGGGTGCTGCAAAGCTTTCTGGTCCGAATATGTATGAGCAGGGTGCAGGCAGGGTTGATCTGTTAGAATCATACGAAATCCTCAAGAGCTACCAACCTCGTGCCAGCATCTTCCCTAGCACTCTCGATTATACAGATTGCCCTTACTCTTGGCCCTTCTGTCGTCAGCCCCTTTATGCAGGTGCCATGCCTGTTATCTTTAATGCTACCATTCTAAATGGAATGGGTGTAATAGGCTACGTTGAAAGTCCACCCACATGGCATCCTTCGGATGATGTAGGGAATCTTTTGAGCATTCACTTTACTTATTCTGAAGTTATTTGGCCTTGGACTGGTTATTTAGCTCTTCACATGCAAATTAAGGAAGAAGGTGCACAATTTTCTGGAGAGATTGGAGGCAATGTTACTCTTAGGGTGTACAGTCCTCCAGCTCTAGGAGAGACGGATACTCGGATTAGTACTTGTGTGCTTCAGTTGAAATTGAATATTGTCCCAACTCCACCAAGATCGAAACGAGTTTTGTGGGATCAGTTTCACAGCATTAAATACCCTCCAGGTTATATCCCTAGAGATTCTTTGGATGTTCGTAATGACATTCTTGACTGGCATGGGGATCACCTGCATACGAATTTTCACATTATGTTCAACATGTTACGAGACGCTGGGTATTATGTCGAAACACTAGGTTCGCCATTTACTTGCTTTGATGCTCTCCGGTATGGTACACTTCTTCTTGTAGATCTTGAAGAAGAGTACTTTCAGGAGGAGATTGAAAAGCTGCGAGATGATGTTCTTAATTCTGGACTGGGATTAGTGGTGTTTTCTGAATGGTATAATGTGGACACAATGGTGAAGATGAGATTCTTTGATGATAACACACGGAGCTGGTGGACTCCAGTCACTGGTGGCGCAAATGTTCCAGCATTGAATGACCTTTTAGCTCCGTTTGGGATTGCATTTGGAGATAAGATTCTGAATGGTGATTTTTCTATTAATGGTGAGCATAGTCGATATGCATCTGGAACGAATATTGTGAGGTTTCCACGAGGTGGTTATGTGCACAAGTTCCCTTTCATGGATAGCTCTGAGAGTGGGGCCACTCAAAATATACTTCGGACTCCTGAGATGACCACGGCAGATTCTCCTATTCTTGGTCTTGTTGAGGTGGGCGAAGGTCGTGTTGTAGTCTATGGAGACTCTAACTGTTTGGACAGCAGTCATATGGTGACAAATTGTTATTGGCTCTTGAGGAAAATATTAGATTTCACCGGTGGAAACATCAAAGACCCCGTGCTTTTCTCAAACTCGGTTAAACAAAGTTCACCTTTACATATAGCAGAGAACCAATTACCGTCTCGTAGAACTGATGTGAATTTCTCTACATATTCTTCTGTTGTGGGAAAGGAATTGATGTGTGGGAGTGACTCCGTGTTTGAAATCTGGGGAACGAAAGGTTACAGTTCACAGGTCAGGGGAACAAACAGGAGATTACCGGGTTATTCCGTGGTTGACTTGGATAGAGGTTTAAATTCTACAGCCGATACTTCCAATTCGAAACGTACCAAATTGAGTGTGGAAAGTAGAAGTGATCCTTTGGGATACTTGGGCCTGTTCTATGGGGACGAGCTTGACATGCCTGTGTTAGTTCCTACTCATTGGCTTGTTCCTGCGATCATTCTTGTAACTGGTCTTCTGGTATTTTTGAGCTTTTGGAGAATCCGACAGAAACGACGGCGGCGAAGGAAAGGATCTGGTTCCGGTCGGTATTCTAATTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0004252 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005788 GO:0005789 GO:0005794 GO:0006464 GO:0006508 GO:0006807 GO:0006950 GO:0006970 GO:0006972 GO:0006984 GO:0006986 GO:0006996 GO:0007033 GO:0007040 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0008237 GO:0009628 GO:0009651 GO:0009889 GO:0009987 GO:0010033 GO:0012505 GO:0016020 GO:0016043 GO:0016787 GO:0017171 GO:0019216 GO:0019218 GO:0019222 GO:0019538 GO:0023052 GO:0030968 GO:0031090 GO:0031293 GO:0031974 GO:0031984 GO:0033554 GO:0033619 GO:0034620 GO:0034976 GO:0035966 GO:0035967 GO:0036211 GO:0036500 GO:0042175 GO:0042221 GO:0042538 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043687 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045540 GO:0046890 GO:0050789 GO:0050794 GO:0050810 GO:0050896 GO:0051716 GO:0062012 GO:0065007 GO:0070011 GO:0070013 GO:0070887 GO:0071310 GO:0071704 GO:0071840 GO:0080090 GO:0080171 GO:0090181 GO:0098588 GO:0098791 GO:0098827 GO:0106118 GO:0140096 GO:1901564 GO:1902930

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

Peptidase_S8

Protein Analysis

1058

Amino Acids

117.48

Weight (kDa)

5.81

Isoelectric Point (pI)

44.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MBTP1_N	PF23001	55 - 128	3.5e-14	Membrane-bound Site-1 Protease Family N-terminal domain
Peptidase_S8	PF00082	210 - 459	1.5e-40	Subtilase family
MBTPS1_3rd	PF23094	503 - 631	9.4e-54	MBTPS1, third Ig-like domain
MBTPS1_4th	PF23090	632 - 903	9.1e-147	MBTPS1, fourth GATase-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002222)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G19660 AT5G19660 AT5G19660
fragaria_vesca	FvH4_1g04670 FvH4_1g04670 FvH4_1g04670 FvH4_1g04670 FvH4_1g04670 FvH4_1g04670 FvH4_1g04670
malus_domestica	MD02G1045500.v1.1 MD15G1183900.v1.1
prunus_persica	Prupe.7G232300_v2.0.a1 Prupe.7G232300_v2.0.a1
pyrus_communis	pycom02g03790 pycom02g03800 pycom02g03810 pycom15g16550
rosa_chinensis	RchiOBHm_Chr2g0090281
rosa_laevigata	RLG00000008526 RLG00000016094 RLG00000021848 RLG00000032088
rosa_multiflora	Rmu_sc0000714.1_g000011 Rmu_sc0004834.1_g000013 Rmu_sc0005276.1_g000005
rosa_roxburghii	Rroxscaffold_2G00085240 Rroxscaffold_2G00151320
rosa_rugosa	Rorug02G0006200
rosa_samantha	Rh1DG037900 Rh2AG052100 Rh2BG050700 Rh2CG052900 Rh2DG052000 Rh5AG367600 Rh5AG473400 Rh5BG377700 Rh5BG493700 Rh5CG401100 Rh5CG517700 Rh5DG391000 Rh5DG505700
rosa_wichuraiana	Rw2G004480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	1565, 2022
AasI	GACNNNNNNGTC	1 cut(s)	484
Acc36I	ACCTGC	2 cut(s)	1565, 2022
AccB1I	GGYRCC	1 cut(s)	1577
AccB7I	CCANNNNNTGG	2 cut(s)	2297, 2444
AccBSI	CCGCTC	1 cut(s)	8
AccII	CGCG	2 cut(s)	18, 148
AclWI	GGATC	8 cut(s)	234, 266, 1200, 1213, 1929, 2016, 2978, 3151
AcoI	YGGCCR	3 cut(s)	461, 522, 645
AcsI	RAATTY	7 cut(s)	106, 139, 348, 663, 2023, 2767, 2923
AcuI	CTGAAG	2 cut(s)	1728, 1850
AcvI	CACGTG	1 cut(s)	1124
AdeI	CACNNNGTG	1 cut(s)	2444
AfaI	GTAC	8 cut(s)	287, 1135, 1219, 1814, 1855, 2119, 2150, 2956
AfiI	CCNNNNNNNGG	8 cut(s)	249, 1034, 1727, 1829, 2297, 2444, 2470, 2546
AflIII	ACRYGT	1 cut(s)	2043
AgeI	ACCGGT	1 cut(s)	2663
AjnI	CCWGG	1 cut(s)	1948
AleI	CACNNNNGTG	2 cut(s)	1247, 2243
Alw21I	GWGCWC	3 cut(s)	213, 1771, 2457
Alw26I	GTCTC	8 cut(s)	149, 326, 570, 800, 1829, 2046, 2583, 2754
Alw44I	GTGCAC	2 cut(s)	1767, 2453
AlwI	GGATC	8 cut(s)	234, 266, 1200, 1213, 1929, 2016, 2978, 3151
AlwNI	CAGNNNCTG	1 cut(s)	2297
AoxI	GGCC	7 cut(s)	461, 522, 645, 1547, 1718, 2490, 3001
ApaLI	GTGCAC	2 cut(s)	1767, 2453
ApeKI	GCWGC	3 cut(s)	1364, 1403, 2173
ApoI	RAATTY	7 cut(s)	106, 139, 348, 663, 2023, 2767, 2923
ArsI	GACNNNNNNTTYG	4 cut(s)	941, 973, 2810, 2842
AseI	ATTAAT	1 cut(s)	2388
AsiGI	ACCGGT	1 cut(s)	2663
AspLEI	GCGC	3 cut(s)	20, 705, 2306
AspS9I	GGNCC	8 cut(s)	935, 949, 1025, 1379, 1418, 1548, 2490, 3001
AsuC2I	CCSGG	3 cut(s)	275, 465, 2889
AsuII	TTCGAA	1 cut(s)	2948
AvaII	GGWCC	5 cut(s)	935, 949, 1025, 1379, 1418
BaeGI	GKGCMC	3 cut(s)	1244, 1771, 2457
BalI	TGGCCA	1 cut(s)	647
BamHI	GGATCC	1 cut(s)	1205
BanI	GGYRCC	1 cut(s)	1577
BanII	GRGCYC	1 cut(s)	213
BarI	GAAGNNNNNNTAC	2 cut(s)	3069, 3101
BauI	CACGAG	2 cut(s)	1494, 2439
BbrPI	CACGTG	1 cut(s)	1124
BbsI	GAAGAC	2 cut(s)	1274, 3077
Bbv12I	GWGCWC	3 cut(s)	213, 1771, 2457
BbvCI	CCTCAGC	1 cut(s)	414
BbvI	GCAGC	3 cut(s)	1351, 1390, 2160
BccI	CCATC	4 cut(s)	80, 239, 443, 656
BceAI	ACGGC	5 cut(s)	44, 495, 999, 2544, 3143
BcgI	CGANNNNNNTGC	4 cut(s)	491, 501, 525, 535
BciT130I	CCWGG	1 cut(s)	1950
BciVI	GTATCC	2 cut(s)	1834, 2987
BclI	TGATCA	1 cut(s)	1099
BcnI	CCSGG	3 cut(s)	275, 465, 2889
BcoDI	GTCTC	8 cut(s)	149, 326, 570, 800, 1829, 2046, 2583, 2754
BfaI	CTAG	4 cut(s)	1512, 1829, 1962, 2078
BfmI	CTRYAG	2 cut(s)	1093, 2928
BfuAI	ACCTGC	2 cut(s)	1565, 2022
BfuI	GTATCC	2 cut(s)	1834, 2987
BglII	AGATCT	2 cut(s)	572, 2134
BisI	GCNGC	8 cut(s)	21, 461, 525, 557, 1365, 1404, 2174, 3131
BlsI	GCNGC	8 cut(s)	22, 462, 526, 558, 1366, 1405, 2175, 3132
BmcAI	AGTACT	2 cut(s)	1855, 2150
Bme1390I	CCNGG	4 cut(s)	275, 465, 1950, 2889
Bme18I	GGWCC	5 cut(s)	935, 949, 1025, 1379, 1418
BmgT120I	GGNCC	8 cut(s)	935, 949, 1025, 1379, 1418, 1548, 2490, 3001
BmiI	GGNNCC	5 cut(s)	872, 1207, 1579, 2491, 3151
BmrFI	CCNGG	4 cut(s)	275, 465, 1950, 2889
BmrI	ACTGGG	1 cut(s)	2210
BmsI	GCATC	8 cut(s)	499, 775, 835, 1134, 1512, 1666, 2092, 2420
BmuI	ACTGGG	1 cut(s)	2210
BoxI	GACNNNNGTC	2 cut(s)	2291, 2610
BpiI	GAAGAC	2 cut(s)	1274, 3077
BpmI	CTGGAG	6 cut(s)	61, 568, 1800, 1806, 1932, 2274
Bpu10I	CCTNAGC	1 cut(s)	414
Bpu14I	TTCGAA	1 cut(s)	2948
BpuEI	CTTGAG	3 cut(s)	561, 1463, 2662
BpuMI	CCSGG	3 cut(s)	275, 465, 2889
BsaAI	YACGTR	1 cut(s)	1124
BsaBI	GATNNNNATC	1 cut(s)	2410
BsaJI	CCNNGG	4 cut(s)	742, 1721, 2526, 2898
BsaWI	WCCGGW	3 cut(s)	2109, 2663, 3152
BsaXI	ACNNNNNCTCC	3 cut(s)	25, 1782, 1812
Bsc4I	CCNNNNNNNGG	8 cut(s)	249, 1034, 1727, 1829, 2297, 2444, 2470, 2546
Bse118I	RCCGGY	1 cut(s)	2663
Bse1I	ACTGG	9 cut(s)	664, 716, 1356, 1732, 2003, 2205, 2291, 2302, 3085
Bse3DI	GCAATG	2 cut(s)	1104, 1801
Bse8I	GATNNNNATC	1 cut(s)	2410
BseBI	CCWGG	1 cut(s)	1950
BseDI	CCNNGG	4 cut(s)	742, 1721, 2526, 2898
BseGI	GGATG	7 cut(s)	379, 391, 514, 916, 1657, 1672, 1981
BseJI	GATNNNNATC	1 cut(s)	2410
BseLI	CCNNNNNNNGG	8 cut(s)	249, 1034, 1727, 1829, 2297, 2444, 2470, 2546
BseMI	GCAATG	2 cut(s)	1104, 1801
BseMII	CTCAG	6 cut(s)	405, 581, 780, 937, 2472, 2508
BseNI	ACTGG	9 cut(s)	664, 716, 1356, 1732, 2003, 2205, 2291, 2302, 3085
BseRI	GAGGAG	3 cut(s)	77, 221, 2174
BseSI	GKGCMC	3 cut(s)	1244, 1771, 2457
BseX3I	CGGCCG	2 cut(s)	461, 522
BseXI	GCAGC	3 cut(s)	1351, 1390, 2160
BseYI	CCCAGC	1 cut(s)	2057
BsgI	GTGCAG	1 cut(s)	1461
Bsh1236I	CGCG	2 cut(s)	18, 148
Bsh1285I	CGRYCG	3 cut(s)	464, 525, 3157
BshFI	GGCC	7 cut(s)	463, 524, 647, 1549, 1720, 2492, 3003
BshNI	GGYRCC	1 cut(s)	1577
BshTI	ACCGGT	1 cut(s)	2663
BsiEI	CGRYCG	3 cut(s)	464, 525, 3157
BsiHKAI	GWGCWC	3 cut(s)	213, 1771, 2457
BsiSI	CCGG	6 cut(s)	275, 464, 2110, 2664, 2888, 3153
BslFI	GGGAC	5 cut(s)	440, 589, 1208, 1871, 3029
BslI	CCNNNNNNNGG	8 cut(s)	249, 1034, 1727, 1829, 2297, 2444, 2470, 2546
BsmAI	GTCTC	8 cut(s)	149, 326, 570, 800, 1829, 2046, 2583, 2754
BsmBI	CGTCTC	4 cut(s)	326, 1829, 2046, 2754
BsmFI	GGGAC	5 cut(s)	440, 589, 1208, 1871, 3029
BsmI	GAATGC	3 cut(s)	385, 881, 1689
BsnI	GGCC	7 cut(s)	463, 524, 647, 1549, 1720, 2492, 3003
Bsp119I	TTCGAA	1 cut(s)	2948
Bsp1286I	GDGCHC	4 cut(s)	213, 1244, 1771, 2457
Bsp1407I	TGTACA	1 cut(s)	1812
BspANI	GGCC	7 cut(s)	463, 524, 647, 1549, 1720, 2492, 3003
BspCNI	CTCAG	6 cut(s)	406, 580, 781, 936, 2473, 2509
BspFNI	CGCG	2 cut(s)	18, 148
BspLI	GGNNCC	5 cut(s)	872, 1207, 1579, 2491, 3151
BspMI	ACCTGC	2 cut(s)	1565, 2022
BspPI	GGATC	8 cut(s)	234, 266, 1200, 1213, 1929, 2016, 2978, 3151
BspQI	GCTCTTC	1 cut(s)	1746
BspT104I	TTCGAA	1 cut(s)	2948
BspT107I	GGYRCC	1 cut(s)	1577
BsrBI	CCGCTC	1 cut(s)	8
BsrDI	GCAATG	2 cut(s)	1104, 1801
BsrFI	RCCGGY	1 cut(s)	2663
BsrGI	TGTACA	1 cut(s)	1812
BsrI	ACTGG	9 cut(s)	664, 716, 1356, 1732, 2003, 2205, 2291, 2302, 3085
BssAI	RCCGGY	1 cut(s)	2663
BssECI	CCNNGG	4 cut(s)	742, 1721, 2526, 2898
BssSI	CACGAG	2 cut(s)	1494, 2439
BssT1I	CCWWGG	2 cut(s)	742, 1721
Bst2BI	CACGAG	2 cut(s)	1494, 2439
Bst2UI	CCWGG	1 cut(s)	1950
Bst4CI	ACNGT	6 cut(s)	337, 1097, 1817, 2600, 2748, 2855
Bst6I	CTCTTC	4 cut(s)	45, 50, 1746, 2139
BstAUI	TGTACA	1 cut(s)	1812
BstBAI	YACGTR	1 cut(s)	1124
BstBI	TTCGAA	1 cut(s)	2948
BstC8I	GCNNGC	4 cut(s)	411, 1336, 1444, 1501
BstDEI	CTNAG	7 cut(s)	414, 567, 789, 923, 1805, 2481, 2517
BstDSI	CCRYGG	2 cut(s)	2526, 2898
BstF5I	GGATG	7 cut(s)	379, 391, 514, 916, 1657, 1672, 1981
BstFNI	CGCG	2 cut(s)	18, 148
BstHHI	GCGC	3 cut(s)	20, 705, 2306
BstMAI	GTCTC	8 cut(s)	149, 326, 570, 800, 1829, 2046, 2583, 2754
BstMCI	CGRYCG	3 cut(s)	464, 525, 3157
BstMWI	GCNNNNNNNGC	4 cut(s)	1083, 1230, 1239, 3028
BstNI	CCWGG	1 cut(s)	1950
BstNSI	RCATGY	3 cut(s)	1750, 2047, 3031
BstPAI	GACNNNNGTC	2 cut(s)	2291, 2610
BstSCI	CCNGG	4 cut(s)	273, 463, 1948, 2887
BstSFI	CTRYAG	2 cut(s)	1093, 2928
BstSLI	GKGCMC	3 cut(s)	1244, 1771, 2457
BstUI	CGCG	2 cut(s)	18, 148
BstV1I	GCAGC	3 cut(s)	1351, 1390, 2160
BstV2I	GAAGAC	2 cut(s)	1274, 3077
BstX2I	RGATCY	4 cut(s)	572, 1205, 2134, 3143
BstXI	CCANNNNNNTGG	1 cut(s)	1653
BstYI	RGATCY	4 cut(s)	572, 1205, 2134, 3143
BstZI	CGGCCG	2 cut(s)	461, 522
BsuI	GTATCC	2 cut(s)	1834, 2987
BsuRI	GGCC	7 cut(s)	463, 524, 647, 1549, 1720, 2492, 3003
BtgI	CCRYGG	2 cut(s)	2526, 2898
BtgZI	GCGATG	2 cut(s)	40, 497
BtsCI	GGATG	7 cut(s)	379, 391, 514, 916, 1657, 1672, 1981
BtsIMutI	CAGTG	6 cut(s)	499, 657, 1102, 1297, 1349, 2295
BveI	ACCTGC	2 cut(s)	1565, 2022
Cac8I	GCNNGC	4 cut(s)	411, 1336, 1444, 1501
CaiI	CAGNNNCTG	1 cut(s)	2297
CfoI	GCGC	3 cut(s)	20, 705, 2306
Cfr10I	RCCGGY	1 cut(s)	2663
Cfr13I	GGNCC	8 cut(s)	935, 949, 1025, 1379, 1418, 1548, 2490, 3001
CpoI	CGGWCCG	1 cut(s)	1025
CseI	GACGC	2 cut(s)	887, 2063
Csp6I	GTAC	8 cut(s)	286, 1134, 1218, 1813, 1854, 2118, 2149, 2955
CspAI	ACCGGT	1 cut(s)	2663
CspCI	CAANNNNNGTGG	2 cut(s)	1242, 1277
CspI	CGGWCCG	1 cut(s)	1025
CviQI	GTAC	8 cut(s)	286, 1134, 1218, 1813, 1854, 2118, 2149, 2955
DdeI	CTNAG	7 cut(s)	414, 567, 789, 923, 1805, 2481, 2517
DraI	TTTAAA	2 cut(s)	1326, 2922
DraIII	CACNNNGTG	1 cut(s)	2444
DrdI	GACNNNNNNGTC	1 cut(s)	484
DseDI	GACNNNNNNGTC	1 cut(s)	484
EaeI	YGGCCR	3 cut(s)	461, 522, 645
EagI	CGGCCG	2 cut(s)	461, 522
Eam1104I	CTCTTC	4 cut(s)	45, 50, 1746, 2139
EarI	CTCTTC	4 cut(s)	45, 50, 1746, 2139
EciI	GGCGGA	2 cut(s)	218, 614
Ecl136II	GAGCTC	1 cut(s)	211
EclXI	CGGCCG	2 cut(s)	461, 522
Eco130I	CCWWGG	2 cut(s)	742, 1721
Eco24I	GRGCYC	1 cut(s)	213
Eco47I	GGWCC	5 cut(s)	935, 949, 1025, 1379, 1418
Eco52I	CGGCCG	2 cut(s)	461, 522
Eco53kI	GAGCTC	1 cut(s)	211
Eco57I	CTGAAG	2 cut(s)	1728, 1850
Eco72I	CACGTG	1 cut(s)	1124
EcoICRI	GAGCTC	1 cut(s)	211
EcoRI	GAATTC	1 cut(s)	663
EcoRII	CCWGG	1 cut(s)	1948
EcoT14I	CCWWGG	2 cut(s)	742, 1721
EcoT22I	ATGCAT	2 cut(s)	514, 2413
EcoT38I	GRGCYC	1 cut(s)	213
ErhI	CCWWGG	2 cut(s)	742, 1721
Esp3I	CGTCTC	4 cut(s)	326, 1829, 2046, 2754
FalI	AAGNNNNNCTT	4 cut(s)	2704, 2736, 2971, 3003
FaqI	GGGAC	5 cut(s)	440, 589, 1208, 1871, 3029
FauI	CCCGC	1 cut(s)	687
FauNDI	CATATG	1 cut(s)	2616
FbaI	TGATCA	1 cut(s)	1099
Fnu4HI	GCNGC	8 cut(s)	21, 461, 525, 557, 1365, 1404, 2174, 3131
FokI	GGATG	7 cut(s)	386, 398, 521, 923, 1644, 1679, 1988
FriOI	GRGCYC	1 cut(s)	213
Fsp4HI	GCNGC	8 cut(s)	21, 461, 525, 557, 1365, 1404, 2174, 3131
FspBI	CTAG	4 cut(s)	1512, 1829, 1962, 2078
GlaI	GCGC	3 cut(s)	19, 704, 2305
GluI	GCNGC	8 cut(s)	21, 461, 525, 557, 1365, 1404, 2174, 3131
GsaI	CCCAGC	1 cut(s)	2061
GsuI	CTGGAG	6 cut(s)	61, 568, 1800, 1806, 1932, 2274
HaeIII	GGCC	7 cut(s)	463, 524, 647, 1549, 1720, 2492, 3003
HapII	CCGG	6 cut(s)	275, 464, 2110, 2664, 2888, 3153
HgaI	GACGC	2 cut(s)	887, 2063
HhaI	GCGC	3 cut(s)	20, 705, 2306
Hin6I	GCGC	3 cut(s)	18, 703, 2304
HinP1I	GCGC	3 cut(s)	18, 703, 2304
HincII	GTYRAC	1 cut(s)	2905
HindII	GTYRAC	1 cut(s)	2905
HindIII	AAGCTT	3 cut(s)	1231, 1370, 1409
HpaII	CCGG	6 cut(s)	275, 464, 2110, 2664, 2888, 3153
Hpy99I	CGWCG	6 cut(s)	53, 179, 295, 328, 506, 3129
HpyCH4III	ACNGT	6 cut(s)	337, 1097, 1817, 2600, 2748, 2855
HpyCH4IV	ACGT	4 cut(s)	174, 1123, 1635, 2953
HpyF10VI	GCNNNNNNNGC	4 cut(s)	1083, 1230, 1239, 3028
HpyF3I	CTNAG	7 cut(s)	414, 567, 789, 923, 1805, 2481, 2517
HpySE526I	ACGT	4 cut(s)	174, 1123, 1635, 2953
HspAI	GCGC	3 cut(s)	18, 703, 2304
Ksp22I	TGATCA	1 cut(s)	1099
LguI	GCTCTTC	1 cut(s)	1746
LmnI	GCTCC	8 cut(s)	13, 208, 216, 354, 700, 811, 2277, 2341
Lsp1109I	GCAGC	3 cut(s)	1351, 1390, 2160
LweI	GCATC	8 cut(s)	499, 775, 835, 1134, 1512, 1666, 2092, 2420
MaeI	CTAG	4 cut(s)	1512, 1829, 1962, 2078
MaeII	ACGT	4 cut(s)	174, 1123, 1635, 2953
MaeIII	GTNAC	9 cut(s)	580, 1119, 1798, 2046, 2293, 2620, 2816, 2849, 3076
MbiI	CCGCTC	1 cut(s)	8
MfeI	CAATTG	2 cut(s)	894, 1011
MflI	RGATCY	4 cut(s)	572, 1205, 2134, 3143
MhlI	GDGCHC	4 cut(s)	213, 1244, 1771, 2457
MlsI	TGGCCA	1 cut(s)	647
MluNI	TGGCCA	1 cut(s)	647
MlyI	GAGTC	5 cut(s)	611, 2281, 2506, 2585, 2813
MmeI	TCCRAC	1 cut(s)	3139
Mox20I	TGGCCA	1 cut(s)	647
Mph1103I	ATGCAT	2 cut(s)	514, 2413
MscI	TGGCCA	1 cut(s)	647
MslI	CAYNNNNRTG	6 cut(s)	998, 1155, 1247, 1651, 2243, 3032
Msp20I	TGGCCA	1 cut(s)	647
MspI	CCGG	6 cut(s)	275, 464, 2110, 2664, 2888, 3153
MspR9I	CCNGG	4 cut(s)	275, 465, 1950, 2889
MunI	CAATTG	2 cut(s)	894, 1011
Mva1269I	GAATGC	3 cut(s)	385, 881, 1689
MvaI	CCWGG	1 cut(s)	1950
MvnI	CGCG	2 cut(s)	18, 148
MwoI	GCNNNNNNNGC	4 cut(s)	1083, 1230, 1239, 3028
NciI	CCSGG	3 cut(s)	275, 465, 2889
NdeI	CATATG	1 cut(s)	2616
NlaIV	GGNNCC	5 cut(s)	872, 1207, 1579, 2491, 3151
NmeAIII	GCCGAG	1 cut(s)	500
NmuCI	GTSAC	4 cut(s)	1119, 2293, 2620, 2816
NsiI	ATGCAT	2 cut(s)	514, 2413
NspI	RCATGY	3 cut(s)	1750, 2047, 3031
NspV	TTCGAA	1 cut(s)	2948
OliI	CACNNNNGTG	2 cut(s)	1247, 2243
PaqCI	CACCTGC	2 cut(s)	1565, 2022
PciI	ACATGT	1 cut(s)	2043
PciSI	GCTCTTC	1 cut(s)	1746
PcsI	WCGNNNNNNNCGW	4 cut(s)	57, 183, 485, 3130
PctI	GAATGC	3 cut(s)	385, 881, 1689
PfeI	GAWTC	9 cut(s)	183, 320, 1463, 1678, 1967, 2256, 2365, 2534, 3111
PflMI	CCANNNNNTGG	2 cut(s)	2297, 2444
PinAI	ACCGGT	1 cut(s)	2663
PkrI	GCNGC	8 cut(s)	22, 462, 526, 558, 1366, 1405, 2175, 3132
PleI	GAGTC	5 cut(s)	610, 2281, 2506, 2585, 2813
PmaCI	CACGTG	1 cut(s)	1124
PmlI	CACGTG	1 cut(s)	1124
PpsI	GAGTC	5 cut(s)	610, 2281, 2506, 2585, 2813
Ppu21I	YACGTR	1 cut(s)	1124
PscI	ACATGT	1 cut(s)	2043
PshAI	GACNNNNGTC	2 cut(s)	2291, 2610
PshBI	ATTAAT	1 cut(s)	2388
Psp124BI	GAGCTC	1 cut(s)	213
Psp6I	CCWGG	1 cut(s)	1948
PspCI	CACGTG	1 cut(s)	1124
PspFI	CCCAGC	1 cut(s)	2057
PspGI	CCWGG	1 cut(s)	1948
PspN4I	GGNNCC	5 cut(s)	872, 1207, 1579, 2491, 3151
PspPI	GGNCC	8 cut(s)	935, 949, 1025, 1379, 1418, 1548, 2490, 3001
PstNI	CAGNNNCTG	1 cut(s)	2297
PsuI	RGATCY	4 cut(s)	572, 1205, 2134, 3143
RsaI	GTAC	8 cut(s)	287, 1135, 1219, 1814, 1855, 2119, 2150, 2956
RsaNI	GTAC	8 cut(s)	286, 1134, 1218, 1813, 1854, 2118, 2149, 2955
RseI	CAYNNNNRTG	6 cut(s)	998, 1155, 1247, 1651, 2243, 3032
Rsr2I	CGGWCCG	1 cut(s)	1025
RsrII	CGGWCCG	1 cut(s)	1025
SacI	GAGCTC	1 cut(s)	213
SapI	GCTCTTC	1 cut(s)	1746
SatI	GCNGC	8 cut(s)	21, 461, 525, 557, 1365, 1404, 2174, 3131
Sau96I	GGNCC	8 cut(s)	935, 949, 1025, 1379, 1418, 1548, 2490, 3001
ScaI	AGTACT	2 cut(s)	1855, 2150
SchI	GAGTC	5 cut(s)	611, 2281, 2506, 2585, 2813
ScrFI	CCNGG	4 cut(s)	275, 465, 1950, 2889
SduI	GDGCHC	4 cut(s)	213, 1244, 1771, 2457
SfaNI	GCATC	8 cut(s)	499, 775, 835, 1134, 1512, 1666, 2092, 2420
SfcI	CTRYAG	2 cut(s)	1093, 2928
SfuI	TTCGAA	1 cut(s)	2948
SgrAI	CRCCGGYG	1 cut(s)	2663
SinI	GGWCC	5 cut(s)	935, 949, 1025, 1379, 1418
SmiMI	CAYNNNNRTG	6 cut(s)	998, 1155, 1247, 1651, 2243, 3032
SmlI	CTYRAG	3 cut(s)	576, 1478, 2641
SmoI	CTYRAG	3 cut(s)	576, 1478, 2641
SspI	AATATT	3 cut(s)	1882, 2425, 2652
SspMI	CTAG	4 cut(s)	1512, 1829, 1962, 2078
SstI	GAGCTC	1 cut(s)	213
StyD4I	CCNGG	4 cut(s)	273, 463, 1948, 2887
StyI	CCWWGG	2 cut(s)	742, 1721
TaaI	ACNGT	6 cut(s)	337, 1097, 1817, 2600, 2748, 2855
TaiI	ACGT	4 cut(s)	177, 1126, 1638, 2956
TatI	WGTACW	5 cut(s)	1133, 1217, 1812, 1853, 2148
TauI	GCSGC	5 cut(s)	23, 463, 527, 559, 3133
TfiI	GAWTC	9 cut(s)	183, 320, 1463, 1678, 1967, 2256, 2365, 2534, 3111
TscAI	CASTG	6 cut(s)	499, 664, 1102, 1297, 1356, 2302
TseFI	GTSAC	4 cut(s)	1119, 2293, 2620, 2816
TseI	GCWGC	3 cut(s)	1364, 1403, 2173
Tsp45I	GTSAC	4 cut(s)	1119, 2293, 2620, 2816
TspDTI	ATGAA	4 cut(s)	18, 735, 1355, 2458
TspGWI	ACGGA	9 cut(s)	308, 1038, 1050, 1203, 1853, 2290, 2328, 2812, 2887
TspRI	CASTG	6 cut(s)	499, 664, 1102, 1297, 1356, 2302
Van91I	CCANNNNNTGG	2 cut(s)	2297, 2444
VneI	GTGCAC	2 cut(s)	1767, 2453
VpaK11BI	GGWCC	5 cut(s)	935, 949, 1025, 1379, 1418
VspI	ATTAAT	1 cut(s)	2388
XapI	RAATTY	7 cut(s)	106, 139, 348, 663, 2023, 2767, 2923
XceI	RCATGY	3 cut(s)	1750, 2047, 3031
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2965
XspI	CTAG	4 cut(s)	1512, 1829, 1962, 2078
ZrmI	AGTACT	2 cut(s)	1855, 2150
Zsp2I	ATGCAT	2 cut(s)	514, 2413

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.