pycom16g06350

CAAX protease self-immunity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0004201
Physical Location & Seq
Reverse (-)
4079654 .. 4082450
2797 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1203 bp
ATGGAGGAGTCCATCAATAGTGCAGCCAAACGGGTAACAAGTAATATTACGGAAACCTCAGGCCAGCCTTATCCCTCTGGGCTCTGCGCAACGGTCGTAAGTCTCGCTGCTCAGCAATTCTGCATGCTCAAAAGTTCGAATACAATATCAAAACCAGCAATGGTGTACACGCTGTTCACATCTGTAGCTCAATCTTCTTTCTCCAGCTTCTCAGACTCAACCAATCTAACTTCTTCCAGAACCCAAATTCCCTCCCTTTTTCTCGACGGCCGCAGAAGCTTCACAGCCACCTTCTCGCCGCGCCGACTAACCCAGCTTTCAAGGCTCAGAAAAGTCAGAAATCCGCCGTGCATTTGCTTTTTCAGCGGCGGAGGAAAGCCCAAAGGCGATTTCCAAGAAAAGGAAAATGAGTCGAAGTGGCGGATTTTGAAGCGGTGGGATGTGCCCTGGGAATGGCAAACGGCGTCGTTAACATCGCTTGCTTGTGGACTGAGTTTTGTTTTGACAGGACTGGTAGAGGCAGCAGCTATACCGTATTTAGGAATTCGAATTCCGGAATTGAGTTTGGATGAAAAGGCAGAGATACTGATTCTCGATCAAGGTTTTACAACTGCAGCTGTACTTGCTGTTCTATATGCTGTTGCCAGCACTTACCAACCACTGCCTGAAGATGTTTATCGCTACGATTTAAGGGAACCTTTCAATCTACAGAAGGGATGGCTATTGTGGGCAGGGATTGGTATTGTTGGTGCCATAGCTGCTATTGCAGTGACAGGGGCTGCATTGTCCTTGTTGAGGGGGGAGAGCCCAGAACGAGAGAAGGACGCTCTAGTTAGCTTGCTTCCACTGATTGGATCATCCGGTATTAGCACTGCTTGCTTGCTAGGCATCACTGGAGTTTTGGCGCCAATTCTTGAAGAAACTGTGTTTCGCGGGTTTTTTATGGTATCCATGACCAAGTGGGTACCTACACCAGTGGCTATTGTAATAAGTGCAGCTGTATTTGCACTTGCACATCTCACTCCGGGGGAGTTTCCCCAGCTTTTTGTGCTAGGCACTGCTCTGGGATTGTCATATGCTCAAACTCGCAACCTTGTAACCCCCATCACAATACATGCTCTCTGGAACTCAGGAGTGATCTTGCTTCTGACCTTCCTTCAGCTACAAGGGTACGATATCAAGGAACTGTTGCAGACAACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abi

Protein Analysis

401

Amino Acids

43.44

Weight (kDa)

8.16

Isoelectric Point (pI)

46.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rce1-like PF02517 292 - 377 5.2e-21 Type II CAAX prenyl endopeptidase Rce1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 88
Acc65I GGTACC 1 cut(s) 964
AccB1I GGYRCC 3 cut(s) 749, 904, 964
AccB7I CCANNNNNTGG 1 cut(s) 851
AccII CGCG 2 cut(s) 301, 933
AccIII TCCGGA 1 cut(s) 553
AciI CCGC 8 cut(s) 271, 299, 344, 366, 369, 421, 433, 933
AclWI GGATC 1 cut(s) 862
AcoI YGGCCR 1 cut(s) 268
AcsI RAATTY 3 cut(s) 246, 543, 549
AcuI CTGAAG 2 cut(s) 687, 1142
AcyI GRCGYC 2 cut(s) 464, 905
AfaI GTAC 4 cut(s) 167, 621, 966, 1172
AfiI CCNNNNNNNGG 5 cut(s) 400, 453, 539, 795, 851
AgsI TTSAA 4 cut(s) 321, 430, 703, 917
AjnI CCWGG 1 cut(s) 446
AjuI GAANNNNNNNTTGG 2 cut(s) 374, 406
AloI GAACNNNNNNTCC 2 cut(s) 232, 264
Alw26I GTCTC 1 cut(s) 107
AlwI GGATC 1 cut(s) 862
AlwNI CAGNNNCTG 1 cut(s) 779
Aor13HI TCCGGA 1 cut(s) 553
AoxI GGCC 2 cut(s) 61, 268
ApeKI GCWGC 8 cut(s) 23, 107, 521, 524, 614, 758, 779, 995
ApoI RAATTY 3 cut(s) 246, 543, 549
Asp718I GGTACC 1 cut(s) 964
AspLEI GCGC 3 cut(s) 89, 303, 907
AsuC2I CCSGG 1 cut(s) 1026
AsuII TTCGAA 2 cut(s) 137, 547
AxyI CCTNAGG 1 cut(s) 58
BaeGI GKGCMC 1 cut(s) 447
BanI GGYRCC 3 cut(s) 749, 904, 964
BanII GRGCYC 2 cut(s) 84, 809
BbvI GCAGC 8 cut(s) 35, 94, 533, 536, 626, 745, 766, 1007
BccI CCATC 3 cut(s) 20, 711, 1112
BceAI ACGGC 3 cut(s) 283, 331, 477
BciT130I CCWGG 1 cut(s) 448
BciVI GTATCC 1 cut(s) 958
BcnI CCSGG 1 cut(s) 1026
BcoDI GTCTC 1 cut(s) 107
BfaI CTAG 3 cut(s) 830, 884, 1052
BfmI CTRYAG 3 cut(s) 183, 612, 707
BfoI RGCGCY 1 cut(s) 908
BfuI GTATCC 1 cut(s) 958
BlpI GCTNAGC 1 cut(s) 111
Bme1390I CCNGG 2 cut(s) 448, 1026
BmiI GGNNCC 4 cut(s) 696, 751, 906, 966
BmrFI CCNGG 2 cut(s) 448, 1026
BmsI GCATC 1 cut(s) 897
BpmI CTGGAG 2 cut(s) 187, 915
Bpu1102I GCTNAGC 1 cut(s) 111
Bpu14I TTCGAA 2 cut(s) 137, 547
BpuMI CCSGG 1 cut(s) 1026
BsaBI GATNNNNATC 1 cut(s) 675
BsaHI GRCGYC 2 cut(s) 464, 905
BsaJI CCNNGG 3 cut(s) 446, 447, 1025
BsaWI WCCGGW 2 cut(s) 553, 860
Bsc4I CCNNNNNNNGG 5 cut(s) 400, 453, 539, 795, 851
Bse1I ACTGG 3 cut(s) 516, 898, 974
Bse21I CCTNAGG 1 cut(s) 58
Bse3DI GCAATG 1 cut(s) 165
Bse8I GATNNNNATC 1 cut(s) 675
BseAI TCCGGA 1 cut(s) 553
BseBI CCWGG 1 cut(s) 448
BseDI CCNNGG 3 cut(s) 446, 447, 1025
BseGI GGATG 4 cut(s) 445, 574, 722, 857
BseJI GATNNNNATC 1 cut(s) 675
BseLI CCNNNNNNNGG 5 cut(s) 400, 453, 539, 795, 851
BseMI GCAATG 1 cut(s) 165
BseMII CTCAG 6 cut(s) 72, 125, 225, 340, 482, 1143
BseNI ACTGG 3 cut(s) 516, 898, 974
BseRI GAGGAG 1 cut(s) 20
BseSI GKGCMC 1 cut(s) 447
BseX3I CGGCCG 1 cut(s) 268
BseXI GCAGC 8 cut(s) 35, 94, 533, 536, 626, 745, 766, 1007
BseYI CCCAGC 2 cut(s) 312, 1038
BsgI GTGCAG 2 cut(s) 42, 1014
Bsh1236I CGCG 2 cut(s) 301, 933
Bsh1285I CGRYCG 2 cut(s) 96, 271
BshFI GGCC 2 cut(s) 63, 270
BshNI GGYRCC 3 cut(s) 749, 904, 964
BsiEI CGRYCG 2 cut(s) 96, 271
BsiSI CCGG 3 cut(s) 554, 861, 1025
BslI CCNNNNNNNGG 5 cut(s) 400, 453, 539, 795, 851
BsmAI GTCTC 1 cut(s) 107
BsnI GGCC 2 cut(s) 63, 270
Bsp119I TTCGAA 2 cut(s) 137, 547
Bsp1286I GDGCHC 3 cut(s) 84, 447, 809
Bsp13I TCCGGA 1 cut(s) 553
Bsp1407I TGTACA 1 cut(s) 165
Bsp143I GATC 3 cut(s) 595, 854, 1137
Bsp1720I GCTNAGC 1 cut(s) 111
BspACI CCGC 8 cut(s) 271, 299, 344, 366, 369, 421, 433, 933
BspANI GGCC 2 cut(s) 63, 270
BspCNI CTCAG 6 cut(s) 71, 124, 224, 339, 483, 1142
BspEI TCCGGA 1 cut(s) 553
BspFNI CGCG 2 cut(s) 301, 933
BspLI GGNNCC 4 cut(s) 696, 751, 906, 966
BspMAI CTGCAG 1 cut(s) 616
BspPI GGATC 1 cut(s) 862
BspT104I TTCGAA 2 cut(s) 137, 547
BspT107I GGYRCC 3 cut(s) 749, 904, 964
BsrDI GCAATG 1 cut(s) 165
BsrGI TGTACA 1 cut(s) 165
BsrI ACTGG 3 cut(s) 516, 898, 974
BssECI CCNNGG 3 cut(s) 446, 447, 1025
BssMI GATC 3 cut(s) 595, 854, 1137
BssNI GRCGYC 2 cut(s) 464, 905
Bst2UI CCWGG 1 cut(s) 448
Bst4CI ACNGT 4 cut(s) 94, 534, 925, 1188
BstACI GRCGYC 2 cut(s) 464, 905
BstAPI GCANNNNNTGC 1 cut(s) 876
BstAUI TGTACA 1 cut(s) 165
BstBI TTCGAA 2 cut(s) 137, 547
BstC8I GCNNGC 7 cut(s) 65, 125, 480, 646, 839, 877, 881
BstDEI CTNAG 6 cut(s) 58, 111, 211, 326, 491, 1129
BstENI CCTNNNNNAGG 1 cut(s) 793
BstF5I GGATG 4 cut(s) 445, 574, 722, 857
BstFNI CGCG 2 cut(s) 301, 933
BstH2I RGCGCY 1 cut(s) 908
BstHHI GCGC 3 cut(s) 89, 303, 907
BstKTI GATC 3 cut(s) 598, 857, 1140
BstMAI GTCTC 1 cut(s) 107
BstMBI GATC 3 cut(s) 595, 854, 1137
BstMCI CGRYCG 2 cut(s) 96, 271
BstNI CCWGG 1 cut(s) 448
BstNSI RCATGY 2 cut(s) 127, 1118
BstSCI CCNGG 2 cut(s) 446, 1024
BstSFI CTRYAG 3 cut(s) 183, 612, 707
BstSLI GKGCMC 1 cut(s) 447
BstUI CGCG 2 cut(s) 301, 933
BstV1I GCAGC 8 cut(s) 35, 94, 533, 536, 626, 745, 766, 1007
BstZI CGGCCG 1 cut(s) 268
Bsu36I CCTNAGG 1 cut(s) 58
BsuI GTATCC 1 cut(s) 958
BsuRI GGCC 2 cut(s) 63, 270
BtgZI GCGATG 1 cut(s) 459
BtsCI GGATG 4 cut(s) 445, 574, 722, 857
BtsI GCAGTG 4 cut(s) 659, 774, 870, 1056
BtsIMutI CAGTG 7 cut(s) 659, 774, 845, 870, 891, 981, 1056
Cac8I GCNNGC 7 cut(s) 65, 125, 480, 646, 839, 877, 881
CaiI CAGNNNCTG 1 cut(s) 779
CfoI GCGC 3 cut(s) 89, 303, 907
CseI GACGC 2 cut(s) 453, 833
Csp6I GTAC 4 cut(s) 166, 620, 965, 1171
CviAII CATG 3 cut(s) 124, 952, 1115
CviQI GTAC 4 cut(s) 166, 620, 965, 1171
DdeI CTNAG 6 cut(s) 58, 111, 211, 326, 491, 1129
DinI GGCGCC 1 cut(s) 906
DpnI GATC 3 cut(s) 597, 856, 1139
DpnII GATC 3 cut(s) 595, 854, 1137
EaeI YGGCCR 1 cut(s) 268
EagI CGGCCG 1 cut(s) 268
EciI GGCGGA 3 cut(s) 333, 384, 436
EclXI CGGCCG 1 cut(s) 268
Eco24I GRGCYC 2 cut(s) 84, 809
Eco32I GATATC 1 cut(s) 1177
Eco52I CGGCCG 1 cut(s) 268
Eco57I CTGAAG 2 cut(s) 687, 1142
Eco81I CCTNAGG 1 cut(s) 58
EcoNI CCTNNNNNAGG 1 cut(s) 793
EcoRI GAATTC 2 cut(s) 543, 549
EcoRII CCWGG 1 cut(s) 446
EcoRV GATATC 1 cut(s) 1177
EcoT38I GRGCYC 2 cut(s) 84, 809
EgeI GGCGCC 1 cut(s) 906
EheI GGCGCC 1 cut(s) 906
FaeI CATG 3 cut(s) 127, 955, 1118
FalI AAGNNNNNCTT 2 cut(s) 682, 714
FatI CATG 3 cut(s) 123, 951, 1114
FauI CCCGC 1 cut(s) 926
FauNDI CATATG 1 cut(s) 1075
FokI GGATG 4 cut(s) 452, 581, 729, 844
FriOI GRGCYC 2 cut(s) 84, 809
FspBI CTAG 3 cut(s) 830, 884, 1052
FspI TGCGCA 1 cut(s) 88
GlaI GCGC 3 cut(s) 88, 302, 906
GsaI CCCAGC 2 cut(s) 316, 1042
GsuI CTGGAG 2 cut(s) 187, 915
HaeII RGCGCY 1 cut(s) 908
HaeIII GGCC 2 cut(s) 63, 270
HapII CCGG 3 cut(s) 554, 861, 1025
HgaI GACGC 2 cut(s) 453, 833
HhaI GCGC 3 cut(s) 89, 303, 907
Hin1I GRCGYC 2 cut(s) 464, 905
Hin1II CATG 3 cut(s) 127, 955, 1118
Hin6I GCGC 3 cut(s) 87, 301, 905
HinP1I GCGC 3 cut(s) 87, 301, 905
HincII GTYRAC 1 cut(s) 471
HindII GTYRAC 1 cut(s) 471
HindIII AAGCTT 1 cut(s) 277
HinfI GANTC 4 cut(s) 8, 215, 410, 589
HpaI GTTAAC 1 cut(s) 471
HpaII CCGG 3 cut(s) 554, 861, 1025
Hpy166II GTNNAC 5 cut(s) 166, 168, 177, 471, 488
Hpy188I TCNGA 4 cut(s) 214, 329, 338, 1149
Hpy188III TCNNGA 7 cut(s) 237, 263, 554, 593, 914, 1123, 1131
Hpy8I GTNNAC 5 cut(s) 166, 168, 177, 471, 488
Hpy99I CGWCG 2 cut(s) 269, 469
HpyAV CCTTC 5 cut(s) 301, 706, 814, 1162, 1166
HpyCH4III ACNGT 4 cut(s) 94, 534, 925, 1188
HpyF3I CTNAG 6 cut(s) 58, 111, 211, 326, 491, 1129
Hsp92I GRCGYC 2 cut(s) 464, 905
Hsp92II CATG 3 cut(s) 127, 955, 1118
HspAI GCGC 3 cut(s) 87, 301, 905
KasI GGCGCC 1 cut(s) 904
Kpn2I TCCGGA 1 cut(s) 553
KpnI GGTACC 1 cut(s) 968
KspAI GTTAAC 1 cut(s) 471
Kzo9I GATC 3 cut(s) 595, 854, 1137
Lsp1109I GCAGC 8 cut(s) 35, 94, 533, 536, 626, 745, 766, 1007
LweI GCATC 1 cut(s) 897
MaeI CTAG 3 cut(s) 830, 884, 1052
MaeIII GTNAC 3 cut(s) 34, 769, 1096
MalI GATC 3 cut(s) 597, 856, 1139
MboI GATC 3 cut(s) 595, 854, 1137
MboII GAAGA 4 cut(s) 186, 225, 680, 929
MhlI GDGCHC 3 cut(s) 84, 447, 809
MluCI AATT 6 cut(s) 116, 246, 543, 549, 557, 909
Mly113I GGCGCC 1 cut(s) 905
MlyI GAGTC 3 cut(s) 17, 209, 419
MnlI CCTC 6 cut(s) 67, 85, 262, 365, 511, 789
MroI TCCGGA 1 cut(s) 553
MseI TTAA 2 cut(s) 470, 689
MspA1I CMGCKG 3 cut(s) 366, 617, 998
MspI CCGG 3 cut(s) 554, 861, 1025
MspR9I CCNGG 2 cut(s) 448, 1026
MvaI CCWGG 1 cut(s) 448
MvnI CGCG 2 cut(s) 301, 933
NarI GGCGCC 1 cut(s) 905
NciI CCSGG 1 cut(s) 1026
NdeI CATATG 1 cut(s) 1075
NdeII GATC 3 cut(s) 595, 854, 1137
NlaIII CATG 3 cut(s) 127, 955, 1118
NlaIV GGNNCC 4 cut(s) 696, 751, 906, 966
NmuCI GTSAC 1 cut(s) 769
NsbI TGCGCA 1 cut(s) 88
NspI RCATGY 2 cut(s) 127, 1118
NspV TTCGAA 2 cut(s) 137, 547
PaeI GCATGC 1 cut(s) 127
PasI CCCWGGG 1 cut(s) 447
PfeI GAWTC 1 cut(s) 589
PflMI CCANNNNNTGG 1 cut(s) 851
PleI GAGTC 3 cut(s) 16, 209, 418
PluTI GGCGCC 1 cut(s) 908
PpsI GAGTC 3 cut(s) 16, 209, 418
Psp6I CCWGG 1 cut(s) 446
PspFI CCCAGC 2 cut(s) 312, 1038
PspGI CCWGG 1 cut(s) 446
PspN4I GGNNCC 4 cut(s) 696, 751, 906, 966
PsrI GAACNNNNNNTAC 4 cut(s) 158, 190, 612, 644
PstI CTGCAG 1 cut(s) 616
PstNI CAGNNNCTG 1 cut(s) 779
PvuII CAGCTG 2 cut(s) 617, 998
RsaI GTAC 4 cut(s) 167, 621, 966, 1172
RsaNI GTAC 4 cut(s) 166, 620, 965, 1171
SaqAI TTAA 2 cut(s) 470, 689
Sau3AI GATC 3 cut(s) 595, 854, 1137
SchI GAGTC 3 cut(s) 17, 209, 419
ScrFI CCNGG 2 cut(s) 448, 1026
SduI GDGCHC 3 cut(s) 84, 447, 809
SfaNI GCATC 1 cut(s) 897
SfcI CTRYAG 3 cut(s) 183, 612, 707
SfoI GGCGCC 1 cut(s) 906
SfuI TTCGAA 2 cut(s) 137, 547
SphI GCATGC 1 cut(s) 127
Sse9I AATT 6 cut(s) 116, 246, 543, 549, 557, 909
SsiI CCGC 8 cut(s) 271, 299, 344, 366, 369, 421, 433, 933
SspDI GGCGCC 1 cut(s) 904
SspI AATATT 1 cut(s) 46
SspMI CTAG 3 cut(s) 830, 884, 1052
StyD4I CCNGG 2 cut(s) 446, 1024
TaaI ACNGT 4 cut(s) 94, 534, 925, 1188
TaqI TCGA 5 cut(s) 137, 264, 413, 547, 594
TasI AATT 6 cut(s) 116, 246, 543, 549, 557, 909
TatI WGTACW 2 cut(s) 165, 619
TauI GCSGC 3 cut(s) 273, 301, 369
TfiI GAWTC 1 cut(s) 589
Tru1I TTAA 2 cut(s) 470, 689
Tru9I TTAA 2 cut(s) 470, 689
TscAI CASTG 7 cut(s) 666, 774, 852, 877, 898, 981, 1063
TseFI GTSAC 1 cut(s) 769
TseI GCWGC 8 cut(s) 23, 107, 521, 524, 614, 758, 779, 995
Tsp45I GTSAC 1 cut(s) 769
TspDTI ATGAA 1 cut(s) 585
TspGWI ACGGA 1 cut(s) 65
TspRI CASTG 7 cut(s) 666, 774, 852, 877, 898, 981, 1063
Van91I CCANNNNNTGG 1 cut(s) 851
XagI CCTNNNNNAGG 1 cut(s) 793
XapI RAATTY 3 cut(s) 246, 543, 549
XceI RCATGY 2 cut(s) 127, 1118
XspI CTAG 3 cut(s) 830, 884, 1052
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.