pycom16g23510

Xylose isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0003254
Physical Location & Seq
Reverse (-)
24529266 .. 24533855
4590 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1434 bp
ATGAAGGCTGGAAGGATTTTGTTGCTTCTTCTTTGTTTGAATGTGGTTACGTTTGGAGTGATTGCTGATTTGCCAACATGCCCTGCATCAGATCTTGGAAGTGGGTGTGGTGATTCTGATGAATGGGAAGGGGAATTTTTCCCCGGCATTCCCAAAATTAAGTATGAGGGCCCTTCTACCAAGAACCCCCTTGCATTCAAGTGGTATAATGCGGATGAGGAGATTCTTGGGAAAAAAATGAAGGATTGGATGAGGTTTAGTGTTGCATTTTGGCACACATTCCGTGGAACAGGAGGTGACCCATTTGGTGCACCTACAAAGAATTGGCCATGGGAGGATGGAACTAATTCTGTGGCTATGGCAAAGAGAAGAATGAGAGCCAACTTTGAGTTCATAAACAAGCTTGGAGTTGATAGGTGGTGTTTCCATGACAGGGATATCGCTCCTGATGGCAAAACCTTGGAGGAATCTAATAAAAACTTGGATGAAGTGGTGGCCCTTGCTAAAGACCTTCAGGGAACCAAAATTCGACCTTTATGGGGCACAGCTCAGCTGTTTTTGCATCCTCGTTACATGCATGGTGGTGCGACTAGCCCTGAAGTAGGTGTATATGCATATGCTGCTGCTCAAGTTAAGAAAGCCATTGAGGTTACACATTATTTAGGGGGCGAAAATTATGTTTTCTGGGGTGGCCGCGAAGGTTACCAGAGTCTCTTGAATACTGACATGGGAAGAGAGCTTGATCATCTGGCAAGGTTTCTTGAAGCTGCTGTTGCCTACAAGAAGAAAATCGGATACAATGGGACATTGTTGATTGAACCCAAGCCTCAAGAACCTACCAAACACCAGTATGACTGGGATGCTGCAACATCAGCAAACTTCCTGCGAAAATATGGCCTTATAGGAGAATTTAAGCTTAACATTGAGTGCAATCATGCAACACTATCTGGTCACAGCTGTTATCATGAGCTTGAAACTGCAAGACTCAATGGTCTACTTGGAAACGTTGATGCAAACACTGGAGATCCTCAGACTGGATGGGATACAGATCAGTTTCTCACGGATATTGCAGAGGCAACTCTGGTTATGCACAGTGTAGTAAAGAATGGAGGAATAGCACCTGGAGGATTCAACTTTGATGCCAAATTACGAAGAGAAAGCACAGATGTTGAGGATTTGTTCATTGCTCATATCGGCGGAATGGATACCCTCGCCCGTGGCCTCCGTAATGTTGCAAAGTTGGTTGAGGATGGTTCTCTACCTGACCTTGTTCGCAAACGATACAAGAGTTTCGATACTGAAATTGGGGCCCTTATAGAGGCTGGTAAGGCTGATTTTGAGTATCTTGAGAAGAAGGCAATAGAATGGGGTGAACCAACAGTTCCTTCTGCCAAGCAGGAACTTGCGGAGATGCTTTTCCAGTCGGTTTTGTAA

Protein Analysis

478

Amino Acids

53.08

Weight (kDa)

5.57

Isoelectric Point (pI)

38.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP_endonuc_2 PF01261 157 - 322 9.3e-06 Xylose isomerase-like TIM barrel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 990
AccI GTMKAC 1 cut(s) 994
AccII CGCG 1 cut(s) 696
AciI CCGC 4 cut(s) 212, 694, 1197, 1406
AclI AACGTT 1 cut(s) 1005
AclWI GGATC 1 cut(s) 1019
AcoI YGGCCR 2 cut(s) 326, 691
AcsI RAATTY 3 cut(s) 134, 525, 908
AcuI CTGAAG 2 cut(s) 497, 618
AfiI CCNNNNNNNGG 5 cut(s) 433, 539, 602, 1034, 1318
AgsI TTSAA 7 cut(s) 40, 199, 718, 764, 818, 974, 1132
AjnI CCWGG 1 cut(s) 1120
AjuI GAANNNNNNNTTGG 4 cut(s) 374, 406, 1369, 1401
AluBI AGCT 8 cut(s) 403, 548, 553, 739, 767, 916, 957, 970
AluI AGCT 8 cut(s) 403, 548, 553, 739, 767, 916, 957, 970
Alw21I GWGCWC 1 cut(s) 313
Alw26I GTCTC 1 cut(s) 716
Alw44I GTGCAC 1 cut(s) 309
AlwI GGATC 1 cut(s) 1019
AoxI GGCC 7 cut(s) 169, 326, 495, 691, 895, 1219, 1308
ApaI GGGCCC 2 cut(s) 173, 1312
ApaLI GTGCAC 1 cut(s) 309
ApeKI GCWGC 4 cut(s) 620, 623, 767, 863
ApoI RAATTY 3 cut(s) 134, 525, 908
Asp700I GAANNNNTTC 1 cut(s) 346
AspS9I GGNCC 5 cut(s) 169, 170, 496, 1308, 1309
AsuC2I CCSGG 1 cut(s) 144
AsuHPI GGTGA 3 cut(s) 122, 308, 1382
BaeGI GKGCMC 4 cut(s) 173, 313, 545, 1312
BaeI ACNNNNGTAYC 2 cut(s) 1273, 1306
BalI TGGCCA 1 cut(s) 328
BanII GRGCYC 2 cut(s) 173, 1312
Bbv12I GWGCWC 1 cut(s) 313
BbvI GCAGC 4 cut(s) 607, 610, 754, 850
BccI CCATC 4 cut(s) 332, 443, 1032, 1244
BciT130I CCWGG 1 cut(s) 1122
BciVI GTATCC 3 cut(s) 788, 1036, 1198
BclI TGATCA 1 cut(s) 742
BcnI CCSGG 1 cut(s) 144
BcoDI GTCTC 1 cut(s) 716
BfaI CTAG 1 cut(s) 591
BfuI GTATCC 3 cut(s) 788, 1036, 1198
BglII AGATCT 1 cut(s) 91
BisI GCNGC 5 cut(s) 621, 624, 694, 768, 864
BlpI GCTNAGC 1 cut(s) 549
BlsI GCNGC 5 cut(s) 622, 625, 695, 769, 865
Bme1390I CCNGG 2 cut(s) 144, 1122
BmgT120I GGNCC 5 cut(s) 169, 170, 496, 1308, 1309
BmiI GGNNCC 4 cut(s) 171, 520, 1309, 1310
BmrFI CCNGG 2 cut(s) 144, 1122
BmrI ACTGGG 1 cut(s) 865
BmsI GCATC 6 cut(s) 95, 571, 850, 1000, 1129, 1401
BmuI ACTGGG 1 cut(s) 865
BpmI CTGGAG 2 cut(s) 1041, 1143
Bpu1102I GCTNAGC 1 cut(s) 549
BpuEI CTTGAG 3 cut(s) 612, 813, 1367
BpuMI CCSGG 1 cut(s) 144
BsaBI GATNNNNATC 1 cut(s) 1047
BsaJI CCNNGG 5 cut(s) 142, 283, 329, 459, 1216
Bsc4I CCNNNNNNNGG 5 cut(s) 433, 539, 602, 1034, 1318
Bse1I ACTGG 5 cut(s) 847, 860, 1024, 1039, 1420
Bse3DI GCAATG 1 cut(s) 1182
Bse8I GATNNNNATC 1 cut(s) 1047
BseBI CCWGG 1 cut(s) 1122
BseDI CCNNGG 5 cut(s) 142, 283, 329, 459, 1216
BseGI GGATG 8 cut(s) 220, 255, 343, 490, 562, 865, 1043, 1255
BseJI GATNNNNATC 1 cut(s) 1047
BseLI CCNNNNNNNGG 5 cut(s) 433, 539, 602, 1034, 1318
BseMI GCAATG 1 cut(s) 1182
BseMII CTCAG 2 cut(s) 563, 1043
BseNI ACTGG 5 cut(s) 847, 860, 1024, 1039, 1420
BseRI GAGGAG 1 cut(s) 233
BseSI GKGCMC 4 cut(s) 173, 313, 545, 1312
BseXI GCAGC 4 cut(s) 607, 610, 754, 850
Bsh1236I CGCG 1 cut(s) 696
BshFI GGCC 7 cut(s) 171, 328, 497, 693, 897, 1221, 1310
BsiHKAI GWGCWC 1 cut(s) 313
BsiSI CCGG 1 cut(s) 144
BslFI GGGAC 1 cut(s) 817
BslI CCNNNNNNNGG 5 cut(s) 433, 539, 602, 1034, 1318
BsmAI GTCTC 1 cut(s) 716
BsmFI GGGAC 1 cut(s) 817
BsmI GAATGC 2 cut(s) 147, 194
BsnI GGCC 7 cut(s) 171, 328, 497, 693, 897, 1221, 1310
Bsp120I GGGCCC 2 cut(s) 169, 1308
Bsp1286I GDGCHC 4 cut(s) 173, 313, 545, 1312
Bsp143I GATC 4 cut(s) 91, 742, 1024, 1048
Bsp1720I GCTNAGC 1 cut(s) 549
Bsp19I CCATGG 1 cut(s) 329
BspACI CCGC 4 cut(s) 212, 694, 1197, 1406
BspANI GGCC 7 cut(s) 171, 328, 497, 693, 897, 1221, 1310
BspCNI CTCAG 2 cut(s) 562, 1042
BspFNI CGCG 1 cut(s) 696
BspHI TCATGA 1 cut(s) 964
BspLI GGNNCC 4 cut(s) 171, 520, 1309, 1310
BspPI GGATC 1 cut(s) 1019
BsrDI GCAATG 1 cut(s) 1182
BsrI ACTGG 5 cut(s) 847, 860, 1024, 1039, 1420
BssECI CCNNGG 5 cut(s) 142, 283, 329, 459, 1216
BssMI GATC 4 cut(s) 91, 742, 1024, 1048
BssT1I CCWWGG 2 cut(s) 329, 459
Bst2UI CCWGG 1 cut(s) 1122
Bst4CI ACNGT 2 cut(s) 1094, 1381
Bst6I CTCTTC 2 cut(s) 727, 1147
BstAPI GCANNNNNTGC 1 cut(s) 620
BstDEI CTNAG 2 cut(s) 549, 1029
BstDSI CCRYGG 3 cut(s) 283, 329, 1216
BstEII GGTNACC 2 cut(s) 296, 701
BstENI CCTNNNNNAGG 2 cut(s) 600, 1316
BstF5I GGATG 8 cut(s) 220, 255, 343, 490, 562, 865, 1043, 1255
BstFNI CGCG 1 cut(s) 696
BstKTI GATC 4 cut(s) 94, 745, 1027, 1051
BstMAI GTCTC 1 cut(s) 716
BstMBI GATC 4 cut(s) 91, 742, 1024, 1048
BstMWI GCNNNNNNNGC 5 cut(s) 559, 620, 773, 872, 1328
BstNI CCWGG 1 cut(s) 1122
BstNSI RCATGY 2 cut(s) 81, 577
BstPI GGTNACC 2 cut(s) 296, 701
BstSCI CCNGG 2 cut(s) 142, 1120
BstSLI GKGCMC 4 cut(s) 173, 313, 545, 1312
BstUI CGCG 1 cut(s) 696
BstV1I GCAGC 4 cut(s) 607, 610, 754, 850
BstX2I RGATCY 2 cut(s) 91, 1024
BstYI RGATCY 2 cut(s) 91, 1024
BsuI GTATCC 3 cut(s) 788, 1036, 1198
BsuRI GGCC 7 cut(s) 171, 328, 497, 693, 897, 1221, 1310
BtgI CCRYGG 3 cut(s) 283, 329, 1216
BtsCI GGATG 8 cut(s) 220, 255, 343, 490, 562, 865, 1043, 1255
BtsIMutI CAGTG 2 cut(s) 1017, 1099
CciI TCATGA 1 cut(s) 964
Cfr13I GGNCC 5 cut(s) 169, 170, 496, 1308, 1309
CviAII CATG 8 cut(s) 78, 330, 428, 574, 578, 727, 935, 965
DdeI CTNAG 2 cut(s) 549, 1029
DpnI GATC 4 cut(s) 93, 744, 1026, 1050
DpnII GATC 4 cut(s) 91, 742, 1024, 1048
DrdI GACNNNNNNGTC 1 cut(s) 990
DseDI GACNNNNNNGTC 1 cut(s) 990
EaeI YGGCCR 2 cut(s) 326, 691
Eam1104I CTCTTC 2 cut(s) 727, 1147
EarI CTCTTC 2 cut(s) 727, 1147
EciI GGCGGA 1 cut(s) 1212
Eco130I CCWWGG 2 cut(s) 329, 459
Eco24I GRGCYC 2 cut(s) 173, 1312
Eco32I GATATC 1 cut(s) 439
Eco57I CTGAAG 2 cut(s) 497, 618
Eco91I GGTNACC 2 cut(s) 296, 701
EcoNI CCTNNNNNAGG 2 cut(s) 600, 1316
EcoO109I RGGNCCY 4 cut(s) 169, 170, 1308, 1309
EcoO65I GGTNACC 2 cut(s) 296, 701
EcoRII CCWGG 1 cut(s) 1120
EcoRV GATATC 1 cut(s) 439
EcoT14I CCWWGG 2 cut(s) 329, 459
EcoT22I ATGCAT 2 cut(s) 579, 616
EcoT38I GRGCYC 2 cut(s) 173, 1312
ErhI CCWWGG 2 cut(s) 329, 459
FaeI CATG 8 cut(s) 81, 333, 431, 577, 581, 730, 938, 968
FaqI GGGAC 1 cut(s) 817
FatI CATG 8 cut(s) 77, 329, 427, 573, 577, 726, 934, 964
FauNDI CATATG 1 cut(s) 616
FbaI TGATCA 1 cut(s) 742
FblI GTMKAC 1 cut(s) 994
Fnu4HI GCNGC 5 cut(s) 621, 624, 694, 768, 864
FokI GGATG 8 cut(s) 227, 262, 350, 497, 549, 872, 1050, 1262
FriOI GRGCYC 2 cut(s) 173, 1312
Fsp4HI GCNGC 5 cut(s) 621, 624, 694, 768, 864
FspBI CTAG 1 cut(s) 591
GluI GCNGC 5 cut(s) 621, 624, 694, 768, 864
GsuI CTGGAG 2 cut(s) 1041, 1143
HaeIII GGCC 7 cut(s) 171, 328, 497, 693, 897, 1221, 1310
HapII CCGG 1 cut(s) 144
Hin1II CATG 8 cut(s) 81, 333, 431, 577, 581, 730, 938, 968
HindIII AAGCTT 2 cut(s) 401, 914
HinfI GANTC 6 cut(s) 113, 223, 467, 709, 984, 1128
HpaII CCGG 1 cut(s) 144
HphI GGTGA 3 cut(s) 122, 308, 1382
Hpy166II GTNNAC 3 cut(s) 311, 995, 1373
Hpy188I TCNGA 4 cut(s) 91, 118, 794, 1032
Hpy188III TCNNGA 6 cut(s) 446, 715, 761, 830, 965, 1346
Hpy8I GTNNAC 3 cut(s) 311, 995, 1373
HpyAV CCTTC 8 cut(s) 6, 122, 183, 235, 521, 692, 1348, 1395
HpyCH4III ACNGT 2 cut(s) 1094, 1381
HpyCH4IV ACGT 2 cut(s) 50, 1005
HpyF10VI GCNNNNNNNGC 5 cut(s) 559, 620, 773, 872, 1328
HpyF3I CTNAG 2 cut(s) 549, 1029
HpySE526I ACGT 2 cut(s) 50, 1005
Hsp92II CATG 8 cut(s) 81, 333, 431, 577, 581, 730, 938, 968
Ksp22I TGATCA 1 cut(s) 742
Kzo9I GATC 4 cut(s) 91, 742, 1024, 1048
LmnI GCTCC 1 cut(s) 448
Lsp1109I GCAGC 4 cut(s) 607, 610, 754, 850
LweI GCATC 6 cut(s) 95, 571, 850, 1000, 1129, 1401
MaeI CTAG 1 cut(s) 591
MaeII ACGT 2 cut(s) 50, 1005
MaeIII GTNAC 6 cut(s) 46, 296, 569, 649, 701, 950
MalI GATC 4 cut(s) 93, 744, 1026, 1050
MboI GATC 4 cut(s) 91, 742, 1024, 1048
MboII GAAGA 6 cut(s) 20, 381, 744, 796, 1164, 1363
MflI RGATCY 2 cut(s) 91, 1024
MhlI GDGCHC 4 cut(s) 173, 313, 545, 1312
MlsI TGGCCA 1 cut(s) 328
MluCI AATT 9 cut(s) 134, 156, 322, 346, 525, 673, 908, 1145, 1302
MluNI TGGCCA 1 cut(s) 328
MlyI GAGTC 2 cut(s) 718, 978
Mox20I TGGCCA 1 cut(s) 328
Mph1103I ATGCAT 2 cut(s) 579, 616
MroXI GAANNNNTTC 1 cut(s) 346
MscI TGGCCA 1 cut(s) 328
MseI TTAA 4 cut(s) 159, 633, 912, 918
MslI CAYNNNNRTG 3 cut(s) 199, 582, 849
Msp20I TGGCCA 1 cut(s) 328
MspA1I CMGCKG 2 cut(s) 553, 957
MspI CCGG 1 cut(s) 144
MspR9I CCNGG 2 cut(s) 144, 1122
Mva1269I GAATGC 2 cut(s) 147, 194
MvaI CCWGG 1 cut(s) 1122
MvnI CGCG 1 cut(s) 696
MwoI GCNNNNNNNGC 5 cut(s) 559, 620, 773, 872, 1328
NciI CCSGG 1 cut(s) 144
NcoI CCATGG 1 cut(s) 329
NdeI CATATG 1 cut(s) 616
NdeII GATC 4 cut(s) 91, 742, 1024, 1048
NlaIII CATG 8 cut(s) 81, 333, 431, 577, 581, 730, 938, 968
NlaIV GGNNCC 4 cut(s) 171, 520, 1309, 1310
NmuCI GTSAC 2 cut(s) 296, 950
NsiI ATGCAT 2 cut(s) 579, 616
NspI RCATGY 2 cut(s) 81, 577
PagI TCATGA 1 cut(s) 964
PctI GAATGC 2 cut(s) 147, 194
PdmI GAANNNNTTC 1 cut(s) 346
PfeI GAWTC 4 cut(s) 113, 223, 467, 1128
PkrI GCNGC 5 cut(s) 622, 625, 695, 769, 865
PleI GAGTC 2 cut(s) 717, 978
PpsI GAGTC 2 cut(s) 717, 978
Psp1406I AACGTT 1 cut(s) 1005
Psp6I CCWGG 1 cut(s) 1120
PspEI GGTNACC 2 cut(s) 296, 701
PspGI CCWGG 1 cut(s) 1120
PspN4I GGNNCC 4 cut(s) 171, 520, 1309, 1310
PspOMI GGGCCC 2 cut(s) 169, 1308
PspPI GGNCC 5 cut(s) 169, 170, 496, 1308, 1309
PsuI RGATCY 2 cut(s) 91, 1024
PvuII CAGCTG 2 cut(s) 553, 957
RseI CAYNNNNRTG 3 cut(s) 199, 582, 849
SaqAI TTAA 4 cut(s) 159, 633, 912, 918
SatI GCNGC 5 cut(s) 621, 624, 694, 768, 864
Sau3AI GATC 4 cut(s) 91, 742, 1024, 1048
Sau96I GGNCC 5 cut(s) 169, 170, 496, 1308, 1309
SchI GAGTC 2 cut(s) 718, 978
ScrFI CCNGG 2 cut(s) 144, 1122
SduI GDGCHC 4 cut(s) 173, 313, 545, 1312
SfaNI GCATC 6 cut(s) 95, 571, 850, 1000, 1129, 1401
SmiMI CAYNNNNRTG 3 cut(s) 199, 582, 849
SmlI CTYRAG 3 cut(s) 627, 828, 1346
SmoI CTYRAG 3 cut(s) 627, 828, 1346
Sse9I AATT 9 cut(s) 134, 156, 322, 346, 525, 673, 908, 1145, 1302
SsiI CCGC 4 cut(s) 212, 694, 1197, 1406
SspMI CTAG 1 cut(s) 591
StyD4I CCNGG 2 cut(s) 142, 1120
StyI CCWWGG 2 cut(s) 329, 459
TaaI ACNGT 2 cut(s) 1094, 1381
TaiI ACGT 2 cut(s) 53, 1008
TaqI TCGA 2 cut(s) 529, 1293
TasI AATT 9 cut(s) 134, 156, 322, 346, 525, 673, 908, 1145, 1302
TauI GCSGC 1 cut(s) 696
TfiI GAWTC 4 cut(s) 113, 223, 467, 1128
Tru1I TTAA 4 cut(s) 159, 633, 912, 918
Tru9I TTAA 4 cut(s) 159, 633, 912, 918
TscAI CASTG 2 cut(s) 1024, 1099
TseFI GTSAC 2 cut(s) 296, 950
TseI GCWGC 4 cut(s) 620, 623, 767, 863
Tsp45I GTSAC 2 cut(s) 296, 950
TspDTI ATGAA 6 cut(s) 17, 135, 254, 382, 501, 1171
TspGWI ACGGA 3 cut(s) 272, 1076, 1214
TspRI CASTG 2 cut(s) 1024, 1099
VneI GTGCAC 1 cut(s) 309
XagI CCTNNNNNAGG 2 cut(s) 600, 1316
XapI RAATTY 3 cut(s) 134, 525, 908
XceI RCATGY 2 cut(s) 81, 577
XmiI GTMKAC 1 cut(s) 994
XmnI GAANNNNTTC 1 cut(s) 346
XspI CTAG 1 cut(s) 591
Zsp2I ATGCAT 2 cut(s) 579, 616
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.