pycom17g00410

GEM-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0004816
Physical Location & Seq
Forward (+)
291141 .. 293037
1897 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1341 bp
ATGGATGAAATATCTTATCTAGAAGTCATAACTGTTGAAGAAAATTATAACTTATTGGCCTTAATAACACGGTTAAGCAACTTCCTCCTCCTCCTGAGTCAACCTTTTCGCTTTCACGGGCCCCGGTCTCCCAACCAATCAAAAGCACTGCAATTTTCTGCAAACCACATCGATCACACCCAAATTCCAATCCAAGAAACTCTCAGTCTCACACCACAAAATCATACATTTTACATTTGTTTTGCTATTTCCTTTCCATTGCTCCGAGCCAAAATGAACTACCCCAACAACCAAGCATCCCAACCTCCCCTCTACCCCTCCGCTTCTCCTCCCACACACCAAGACACAAACCCTTTTCTCCCAACACCTCCCGCTGAAGACATCAACCCTTTTCATCAAACACCTCCGCCAATGCCACCATCCTTGTCCGAAGAAATACCTCCTCACGATGCATCCTCACCTCAGAATGCTAAAACCCAAGAACCAAAACAAACTACTCATGATCAAGATATTCCAACCTCATCAAGCGGCTCATCTCCTCCGTCAATCCCACCAACTCCAAGTGAGGAAGAAAAATCAGCGCAGTGGGGGACTCACGTCATGGGGCACCCTGCCGTCCCAACTAGCCATCCGGACAACAAAAAAGCAGCTCTGTGGGGCGCTGCCGGAACCGAGCAAGCCCAGAATCCCTACCTCCAGTACAAACCCCTTGACAAGTCCAACAACACCAGTCCAAAGGAATCCATACTCCAAGTCTTCAACTCCTGGAGCAACAAGGCCGAGTCCATGGCCAACAACATCTGGCACAACTTGAAAACGGGGACATCGGTGTCTGGAGCTGCGTGGGCGAAGATGAACCTGACTGCCAAAGCAATAACAGGAGGAGGATTTGAGGCTCTGTACAAGCAGACATTTGCGACTTATCCCAACGAGAAGCTGCATAAGTCATTTGCCAGTTATCTCTCCACATCCACGGGACCAGTTGCCGGAACTCTTTACCTCTCTAATATCCATACAGCTTTCTGCAGCGACCGCCCTTTGTCCTTCACCGCCCCCTCCGGCCAGGTCACCTGGAGCTACTACAAAATAATGGTACCATTGGGAAATATTGCAACTATCAATCCAGTGGTGATGAGGGAAAATCCAAGGGAAAAGTACATCCAGATTGTGACGATCGATGGCCATGATTTCTGGTTTATGGGTTTTGTTAATTACGAAAAGGCGTCGAGGCATTTGACCCAAAGTATTTCAACCTTTGTGGCATCTGGAGTTGCCGTCAAACCAGCCGTTGCTGCTGACAATGACATTGCATACCAGAAGCCTGACAAGGCTGACTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAM

Protein Analysis

447

Amino Acids

49.21

Weight (kDa)

6.34

Isoelectric Point (pI)

50.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 302 - 413 8.1e-12 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 48
AasI GACNNNNNNGTC 1 cut(s) 829
Acc65I GGTACC 1 cut(s) 1093
AccB1I GGYRCC 2 cut(s) 606, 1093
AccIII TCCGGA 1 cut(s) 631
AciI CCGC 6 cut(s) 321, 372, 407, 528, 1033, 1050
AcoI YGGCCR 3 cut(s) 789, 1060, 1180
AcsI RAATTY 1 cut(s) 183
AcuI CTGAAG 1 cut(s) 396
AcyI GRCGYC 1 cut(s) 1223
AfaI GTAC 4 cut(s) 701, 902, 1095, 1157
AfiI CCNNNNNNNGG 1 cut(s) 986
AgsI TTSAA 4 cut(s) 38, 760, 814, 1251
AjiI CACGTC 1 cut(s) 598
AjnI CCWGG 3 cut(s) 764, 1062, 1070
AluBI AGCT 5 cut(s) 650, 839, 937, 1019, 1077
AluI AGCT 5 cut(s) 650, 839, 937, 1019, 1077
Alw26I GTCTC 2 cut(s) 132, 212
Aor13HI TCCGGA 1 cut(s) 631
AoxI GGCC 6 cut(s) 57, 119, 777, 789, 1060, 1180
ApaI GGGCCC 1 cut(s) 123
ApeKI GCWGC 6 cut(s) 647, 662, 839, 937, 1026, 1292
ApoI RAATTY 1 cut(s) 183
Asp718I GGTACC 1 cut(s) 1093
AspLEI GCGC 2 cut(s) 583, 662
AspS9I GGNCC 3 cut(s) 119, 120, 977
AsuC2I CCSGG 1 cut(s) 124
AsuHPI GGTGA 4 cut(s) 450, 1039, 1060, 1141
AvaII GGWCC 1 cut(s) 977
BaeGI GKGCMC 2 cut(s) 123, 609
BalI TGGCCA 2 cut(s) 791, 1182
BanI GGYRCC 2 cut(s) 606, 1093
BanII GRGCYC 1 cut(s) 123
BbsI GAAGAC 2 cut(s) 384, 748
BbvI GCAGC 6 cut(s) 649, 659, 826, 924, 1038, 1279
BccI CCATC 3 cut(s) 427, 636, 1172
BceAI ACGGC 3 cut(s) 599, 1259, 1271
BciT130I CCWGG 3 cut(s) 766, 1064, 1072
BclI TGATCA 1 cut(s) 502
BcnI CCSGG 1 cut(s) 124
BcoDI GTCTC 2 cut(s) 132, 212
BfaI CTAG 2 cut(s) 20, 624
BfmI CTRYAG 1 cut(s) 1024
BfoI RGCGCY 1 cut(s) 663
BisI GCNGC 7 cut(s) 529, 648, 663, 840, 938, 1027, 1293
BlsI GCNGC 7 cut(s) 530, 649, 664, 841, 939, 1028, 1294
Bme1390I CCNGG 4 cut(s) 124, 766, 1064, 1072
Bme18I GGWCC 1 cut(s) 977
BmgBI CACGTC 1 cut(s) 598
BmgT120I GGNCC 3 cut(s) 119, 120, 977
BmiI GGNNCC 6 cut(s) 121, 122, 608, 670, 978, 1095
BmrFI CCNGG 4 cut(s) 124, 766, 1064, 1072
BmsI GCATC 4 cut(s) 305, 439, 461, 1271
BoxI GACNNNNGTC 1 cut(s) 596
BpiI GAAGAC 2 cut(s) 384, 748
BpmI CTGGAG 5 cut(s) 680, 787, 855, 1093, 1287
BpuMI CCSGG 1 cut(s) 124
Bsa29I ATCGAT 2 cut(s) 171, 1176
BsaHI GRCGYC 1 cut(s) 1223
BsaI GGTCTC 1 cut(s) 132
BsaJI CCNNGG 4 cut(s) 122, 786, 972, 1145
BsaWI WCCGGW 1 cut(s) 631
BsaXI ACNNNNNCTCC 2 cut(s) 1260, 1290
Bsc4I CCNNNNNNNGG 1 cut(s) 986
Bse1I ACTGG 5 cut(s) 697, 729, 954, 980, 1124
Bse3DI GCAATG 2 cut(s) 257, 1305
BseAI TCCGGA 1 cut(s) 631
BseBI CCWGG 3 cut(s) 766, 1064, 1072
BseCI ATCGAT 2 cut(s) 171, 1176
BseDI CCNNGG 4 cut(s) 122, 786, 972, 1145
BseGI GGATG 7 cut(s) 10, 296, 419, 452, 628, 968, 1158
BseLI CCNNNNNNNGG 1 cut(s) 986
BseMI GCAATG 2 cut(s) 257, 1305
BseMII CTCAG 3 cut(s) 86, 217, 476
BseNI ACTGG 5 cut(s) 697, 729, 954, 980, 1124
BseRI GAGGAG 6 cut(s) 77, 80, 318, 432, 528, 897
BseSI GKGCMC 2 cut(s) 123, 609
BseXI GCAGC 6 cut(s) 649, 659, 826, 924, 1038, 1279
Bsh1285I CGRYCG 2 cut(s) 1033, 1176
BshFI GGCC 6 cut(s) 59, 121, 779, 791, 1062, 1182
BshNI GGYRCC 2 cut(s) 606, 1093
BshVI ATCGAT 2 cut(s) 171, 1176
BsiEI CGRYCG 2 cut(s) 1033, 1176
BsiSI CCGG 5 cut(s) 124, 632, 666, 987, 1059
BslFI GGGAC 4 cut(s) 602, 604, 835, 990
BslI CCNNNNNNNGG 1 cut(s) 986
BsmAI GTCTC 2 cut(s) 132, 212
BsmFI GGGAC 4 cut(s) 602, 604, 835, 990
BsmI GAATGC 1 cut(s) 472
BsnI GGCC 6 cut(s) 59, 121, 779, 791, 1062, 1182
Bso31I GGTCTC 1 cut(s) 132
Bsp120I GGGCCC 1 cut(s) 119
Bsp1286I GDGCHC 2 cut(s) 123, 609
Bsp13I TCCGGA 1 cut(s) 631
Bsp1407I TGTACA 1 cut(s) 900
Bsp143I GATC 3 cut(s) 172, 502, 1173
Bsp19I CCATGG 1 cut(s) 786
BspACI CCGC 6 cut(s) 321, 372, 407, 528, 1033, 1050
BspANI GGCC 6 cut(s) 59, 121, 779, 791, 1062, 1182
BspCNI CTCAG 3 cut(s) 87, 216, 475
BspDI ATCGAT 2 cut(s) 171, 1176
BspEI TCCGGA 1 cut(s) 631
BspHI TCATGA 1 cut(s) 499
BspLI GGNNCC 6 cut(s) 121, 122, 608, 670, 978, 1095
BspMAI CTGCAG 1 cut(s) 1028
BspT107I GGYRCC 2 cut(s) 606, 1093
BspTNI GGTCTC 1 cut(s) 132
BsrDI GCAATG 2 cut(s) 257, 1305
BsrGI TGTACA 1 cut(s) 900
BsrI ACTGG 5 cut(s) 697, 729, 954, 980, 1124
BssECI CCNNGG 4 cut(s) 122, 786, 972, 1145
BssMI GATC 3 cut(s) 172, 502, 1173
BssNI GRCGYC 1 cut(s) 1223
BssT1I CCWWGG 2 cut(s) 786, 1145
Bst2UI CCWGG 3 cut(s) 766, 1064, 1072
Bst4CI ACNGT 2 cut(s) 34, 72
BstACI GRCGYC 1 cut(s) 1223
BstAUI TGTACA 1 cut(s) 900
BstC8I GCNNGC 1 cut(s) 678
BstDEI CTNAG 3 cut(s) 95, 203, 462
BstDSI CCRYGG 2 cut(s) 786, 972
BstEII GGTNACC 1 cut(s) 1066
BstF5I GGATG 7 cut(s) 10, 296, 419, 452, 628, 968, 1158
BstH2I RGCGCY 1 cut(s) 663
BstHHI GCGC 2 cut(s) 583, 662
BstKTI GATC 3 cut(s) 175, 505, 1176
BstMAI GTCTC 2 cut(s) 132, 212
BstMBI GATC 3 cut(s) 172, 502, 1173
BstMCI CGRYCG 2 cut(s) 1033, 1176
BstMWI GCNNNNNNNGC 3 cut(s) 845, 1032, 1292
BstNI CCWGG 3 cut(s) 766, 1064, 1072
BstPAI GACNNNNGTC 1 cut(s) 596
BstPI GGTNACC 1 cut(s) 1066
BstSCI CCNGG 4 cut(s) 122, 764, 1062, 1070
BstSFI CTRYAG 1 cut(s) 1024
BstSLI GKGCMC 2 cut(s) 123, 609
BstV1I GCAGC 6 cut(s) 649, 659, 826, 924, 1038, 1279
BstV2I GAAGAC 2 cut(s) 384, 748
Bsu15I ATCGAT 2 cut(s) 171, 1176
BsuRI GGCC 6 cut(s) 59, 121, 779, 791, 1062, 1182
BsuTUI ATCGAT 2 cut(s) 171, 1176
BtgI CCRYGG 2 cut(s) 786, 972
BtrI CACGTC 1 cut(s) 598
BtsCI GGATG 7 cut(s) 10, 296, 419, 452, 628, 968, 1158
BtsI GCAGTG 2 cut(s) 146, 590
BtsIMutI CAGTG 3 cut(s) 146, 590, 1131
Cac8I GCNNGC 1 cut(s) 678
CciI TCATGA 1 cut(s) 499
CfoI GCGC 2 cut(s) 583, 662
Cfr13I GGNCC 3 cut(s) 119, 120, 977
ClaI ATCGAT 2 cut(s) 171, 1176
CseI GACGC 1 cut(s) 1212
Csp6I GTAC 4 cut(s) 700, 901, 1094, 1156
CspCI CAANNNNNGTGG 4 cut(s) 405, 440, 1239, 1274
CviAII CATG 4 cut(s) 500, 601, 787, 1184
CviQI GTAC 4 cut(s) 700, 901, 1094, 1156
DdeI CTNAG 3 cut(s) 95, 203, 462
DpnI GATC 3 cut(s) 174, 504, 1175
DpnII GATC 3 cut(s) 172, 502, 1173
DrdI GACNNNNNNGTC 1 cut(s) 829
DseDI GACNNNNNNGTC 1 cut(s) 829
EaeI YGGCCR 3 cut(s) 789, 1060, 1180
EciI GGCGGA 1 cut(s) 396
Eco130I CCWWGG 2 cut(s) 786, 1145
Eco24I GRGCYC 1 cut(s) 123
Eco31I GGTCTC 1 cut(s) 132
Eco47I GGWCC 1 cut(s) 977
Eco57I CTGAAG 1 cut(s) 396
Eco91I GGTNACC 1 cut(s) 1066
EcoO109I RGGNCCY 1 cut(s) 120
EcoO65I GGTNACC 1 cut(s) 1066
EcoRII CCWGG 3 cut(s) 764, 1062, 1070
EcoT14I CCWWGG 2 cut(s) 786, 1145
EcoT22I ATGCAT 1 cut(s) 454
EcoT38I GRGCYC 1 cut(s) 123
ErhI CCWWGG 2 cut(s) 786, 1145
FaeI CATG 4 cut(s) 503, 604, 790, 1187
FaqI GGGAC 4 cut(s) 602, 604, 835, 990
FatI CATG 4 cut(s) 499, 600, 786, 1183
FauI CCCGC 1 cut(s) 379
FbaI TGATCA 1 cut(s) 502
Fnu4HI GCNGC 7 cut(s) 529, 648, 663, 840, 938, 1027, 1293
FokI GGATG 7 cut(s) 17, 283, 406, 439, 615, 955, 1145
FriOI GRGCYC 1 cut(s) 123
Fsp4HI GCNGC 7 cut(s) 529, 648, 663, 840, 938, 1027, 1293
FspBI CTAG 2 cut(s) 20, 624
GlaI GCGC 2 cut(s) 582, 661
GluI GCNGC 7 cut(s) 529, 648, 663, 840, 938, 1027, 1293
GsuI CTGGAG 5 cut(s) 680, 787, 855, 1093, 1287
HaeII RGCGCY 1 cut(s) 663
HaeIII GGCC 6 cut(s) 59, 121, 779, 791, 1062, 1182
HapII CCGG 5 cut(s) 124, 632, 666, 987, 1059
HgaI GACGC 1 cut(s) 1212
HhaI GCGC 2 cut(s) 583, 662
Hin1I GRCGYC 1 cut(s) 1223
Hin1II CATG 4 cut(s) 503, 604, 790, 1187
Hin6I GCGC 2 cut(s) 581, 660
HinP1I GCGC 2 cut(s) 581, 660
HincII GTYRAC 1 cut(s) 101
HindII GTYRAC 1 cut(s) 101
HinfI GANTC 5 cut(s) 97, 592, 685, 740, 782
HpaII CCGG 5 cut(s) 124, 632, 666, 987, 1059
HphI GGTGA 4 cut(s) 450, 1039, 1060, 1141
Hpy166II GTNNAC 1 cut(s) 101
Hpy188I TCNGA 3 cut(s) 266, 430, 465
Hpy188III TCNNGA 9 cut(s) 20, 94, 446, 500, 506, 632, 834, 1162, 1266
Hpy8I GTNNAC 1 cut(s) 101
Hpy99I CGWCG 1 cut(s) 1228
HpyAV CCTTC 1 cut(s) 1054
HpyCH4III ACNGT 2 cut(s) 34, 72
HpyCH4IV ACGT 1 cut(s) 597
HpyCH4V TGCA 7 cut(s) 151, 161, 452, 940, 1026, 1112, 1310
HpyF10VI GCNNNNNNNGC 3 cut(s) 845, 1032, 1292
HpyF3I CTNAG 3 cut(s) 95, 203, 462
HpySE526I ACGT 1 cut(s) 597
Hsp92I GRCGYC 1 cut(s) 1223
Hsp92II CATG 4 cut(s) 503, 604, 790, 1187
HspAI GCGC 2 cut(s) 581, 660
Kpn2I TCCGGA 1 cut(s) 631
KpnI GGTACC 1 cut(s) 1097
Ksp22I TGATCA 1 cut(s) 502
Kzo9I GATC 3 cut(s) 172, 502, 1173
LmnI GCTCC 4 cut(s) 267, 768, 836, 1074
Lsp1109I GCAGC 6 cut(s) 649, 659, 826, 924, 1038, 1279
LweI GCATC 4 cut(s) 305, 439, 461, 1271
MaeI CTAG 2 cut(s) 20, 624
MaeII ACGT 1 cut(s) 597
MaeIII GTNAC 2 cut(s) 1066, 1168
MalI GATC 3 cut(s) 174, 504, 1175
MboI GATC 3 cut(s) 172, 502, 1173
MboII GAAGA 6 cut(s) 50, 389, 443, 581, 748, 862
MhlI GDGCHC 2 cut(s) 123, 609
MlsI TGGCCA 2 cut(s) 791, 1182
MluCI AATT 4 cut(s) 43, 152, 183, 1210
MluNI TGGCCA 2 cut(s) 791, 1182
MlyI GAGTC 3 cut(s) 106, 586, 791
MmeI TCCRAC 2 cut(s) 539, 744
Mox20I TGGCCA 2 cut(s) 791, 1182
Mph1103I ATGCAT 1 cut(s) 454
MroI TCCGGA 1 cut(s) 631
MscI TGGCCA 2 cut(s) 791, 1182
MseI TTAA 3 cut(s) 62, 74, 1209
Msp20I TGGCCA 2 cut(s) 791, 1182
MspA1I CMGCKG 1 cut(s) 374
MspI CCGG 5 cut(s) 124, 632, 666, 987, 1059
MspR9I CCNGG 4 cut(s) 124, 766, 1064, 1072
Mva1269I GAATGC 1 cut(s) 472
MvaI CCWGG 3 cut(s) 766, 1064, 1072
MwoI GCNNNNNNNGC 3 cut(s) 845, 1032, 1292
NciI CCSGG 1 cut(s) 124
NcoI CCATGG 1 cut(s) 786
NdeII GATC 3 cut(s) 172, 502, 1173
NlaIII CATG 4 cut(s) 503, 604, 790, 1187
NlaIV GGNNCC 6 cut(s) 121, 122, 608, 670, 978, 1095
NmeAIII GCCGAG 1 cut(s) 805
NmuCI GTSAC 2 cut(s) 1066, 1168
NsiI ATGCAT 1 cut(s) 454
PagI TCATGA 1 cut(s) 499
PctI GAATGC 1 cut(s) 472
PfeI GAWTC 2 cut(s) 685, 740
PfoI TCCNGGA 1 cut(s) 764
PkrI GCNGC 7 cut(s) 530, 649, 664, 841, 939, 1028, 1294
Ple19I CGATCG 1 cut(s) 1176
PleI GAGTC 3 cut(s) 105, 586, 790
PpsI GAGTC 3 cut(s) 105, 586, 790
PshAI GACNNNNGTC 1 cut(s) 596
PsiI TTATAA 1 cut(s) 48
Psp6I CCWGG 3 cut(s) 764, 1062, 1070
PspEI GGTNACC 1 cut(s) 1066
PspGI CCWGG 3 cut(s) 764, 1062, 1070
PspN4I GGNNCC 6 cut(s) 121, 122, 608, 670, 978, 1095
PspOMI GGGCCC 1 cut(s) 119
PspPI GGNCC 3 cut(s) 119, 120, 977
PstI CTGCAG 1 cut(s) 1028
PvuI CGATCG 1 cut(s) 1176
RsaI GTAC 4 cut(s) 701, 902, 1095, 1157
RsaNI GTAC 4 cut(s) 700, 901, 1094, 1156
SaqAI TTAA 3 cut(s) 62, 74, 1209
SatI GCNGC 7 cut(s) 529, 648, 663, 840, 938, 1027, 1293
Sau3AI GATC 3 cut(s) 172, 502, 1173
Sau96I GGNCC 3 cut(s) 119, 120, 977
SchI GAGTC 3 cut(s) 106, 586, 791
ScrFI CCNGG 4 cut(s) 124, 766, 1064, 1072
SduI GDGCHC 2 cut(s) 123, 609
SfaNI GCATC 4 cut(s) 305, 439, 461, 1271
SfcI CTRYAG 1 cut(s) 1024
SinI GGWCC 1 cut(s) 977
Sse9I AATT 4 cut(s) 43, 152, 183, 1210
SsiI CCGC 6 cut(s) 321, 372, 407, 528, 1033, 1050
SspI AATATT 1 cut(s) 1108
SspMI CTAG 2 cut(s) 20, 624
StyD4I CCNGG 4 cut(s) 122, 764, 1062, 1070
StyI CCWWGG 2 cut(s) 786, 1145
TaaI ACNGT 2 cut(s) 34, 72
TaiI ACGT 1 cut(s) 600
TaqI TCGA 3 cut(s) 171, 1176, 1226
TasI AATT 4 cut(s) 43, 152, 183, 1210
TatI WGTACW 3 cut(s) 699, 900, 1155
TauI GCSGC 1 cut(s) 531
TfiI GAWTC 2 cut(s) 685, 740
Tru1I TTAA 3 cut(s) 62, 74, 1209
Tru9I TTAA 3 cut(s) 62, 74, 1209
TscAI CASTG 3 cut(s) 153, 590, 1131
TseFI GTSAC 2 cut(s) 1066, 1168
TseI GCWGC 6 cut(s) 647, 662, 839, 937, 1026, 1292
Tsp45I GTSAC 2 cut(s) 1066, 1168
TspDTI ATGAA 4 cut(s) 21, 290, 383, 869
TspGWI ACGGA 1 cut(s) 531
TspRI CASTG 3 cut(s) 153, 590, 1131
VpaK11BI GGWCC 1 cut(s) 977
XapI RAATTY 1 cut(s) 183
XbaI TCTAGA 1 cut(s) 19
XspI CTAG 2 cut(s) 20, 624
Zsp2I ATGCAT 1 cut(s) 454
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.