pycom17g01200

Ankyrin repeats (many copies)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0002610
Physical Location & Seq
Forward (+)
839788 .. 842263
2476 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1782 bp
ATGGCCTCTGATTTGATTCGGCCCCATATTGCTGTGCATGCTATTGTCACAGCCAGCTGCAGGGGGTTTGCTGATGTGGTGGACACTCTCATGAAGTGTGGTGTGGATGCAAGTGCAGCTGATAGGATGCTGCTTCAATCGTCTAAGCCAGCTCTTCACACTAATGTTGACTGTAGTGCACTTGTGGCTGCAGTTGTGAGTAGGCAGGTCCCAATCGTACGTTTGCTGCTACAGGCCGGTGTGAGGACGGATGTCAATGTGAGGTTGGGAGCATGGTCATGGGACCCAACTACGGGGGAAGAGTTTCGGGTTGGGGCAGGGTTGGCGGAGCCATATCCCATTACCTGGTGTGCAGTGGAGTATTTTGAAGCAAGCGGTTCTGTCTTGCACATGCTCCTCCAAGGCCTTTCTCCGGATACCCCTCATTGTGGAAGAACTCTCCTGCACCACGCTATTCTATGCGGTAATGCAGGAGCTGTCCGTGTGCTCTTAAGTTGTGGTGCCAATGTAGAATCACCCGTTAAAGCAACTGGTGGTACCATGTTTAATCCGATACACATGGCTGCCCGCCTTGGTTTGCCAACAATCGTTCAATGTTTAACTGAATCTGGATGTGATATGAACTCCAAGACTGATTCTGGTGAGGCAGCTGTAATGATCTGCACAAAATACAAGCACGAAGAATGTCTGAGAGTATTGGCGGCAGCTGGTGCTGATTTTGGCTTGGTTAATGCGGCTGGTCAGTCTGTTAGTTCGATTGCCAGGGCAGCTAGGTGGTCACTTGGTTTTCAGCAGGCGTTGATGTGTATAATGAGTGGCGGAAAGATGCCAAAATCCAGCAACTTTTCTGTTTTCTCTCCCCTAATTTTTGCAGCTCAAGCCGGCAATATTGAGGCCTTGAAAGCAGTAGTTGGTTCAGGAGAATTTGACATCGATTATCAAGATGATAAAGGTTTCACGGCCGTTATGATCACTGCTTTGAAGGGGCACGTTGAAGCATTCAGGTTTCTGGTTTATGCAGGGGCTGATGTAAAACTGTGTAACAAGTCTGGTGAGACTGCAATTACTCTATCAGAAATAAGCCAAAACCGTGACCTATTTGAGAAGGTAATGCTCGAATATGCACTAGAAAAGGGCAATCGTTACGCTGGAGGGTTCTATGCTCTGCATTGTGCAGCACGCCGTGGGGATGTGGATGCAGTCAAATTGTTAACAAGTAAGGGATATGATGTGAATGTCCCTGACGGAGATGGTTACACTCCGCTCATGTTAGCGGCTAGGGAAGGTTACGGTCTCATGTGTGAACTCTTGATATCTCACGGGGCAAACTTGGATATCAAGAATGCCAAAGGAGAAACACCGCTTTCTCTTGCAAGGAAAAGCGGCGGTGGTTTGAAAAATGAGGCAGAGCGGGTGATACTAGATGAGCTAGCTCGCAAGCTGGTGCTAGGTGGGGCGCGTGTCCAGAAGCATACGAAGGGAGGGAAAGGCAGCCCTCATGGGAAAGAGATGAGGATGGTGGGAACTGTAGGGGTGCTCTGCTGGGGGAAATCAAACCGGAGAAATGTGATATGCCGGGAGGCGGAGGTGGGGCCTAGTCCAGCCTTTAGGAGGAATAGGAGGAGCAAGAGCGATGCAGATGAACCCGGAGTGTTTAGGGTGGTGACTACCAAGAACAAGGAGGTGCATTTTGTGTGTGAAGGCGGTGAAAGCGCCGAGCTTTGGGTGAGGGGTATAAAGCTTGTGACCAAGGAGGCTCTTTCAGTAATCGGAGCATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000278 GO:0000281 GO:0000902 GO:0000904 GO:0000910 GO:0001508 GO:0002028 GO:0003008 GO:0003254 GO:0003674 GO:0005198 GO:0005200 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005794 GO:0005829 GO:0005856 GO:0005886 GO:0005911 GO:0006810 GO:0006888 GO:0006892 GO:0006893 GO:0006996 GO:0007009 GO:0007010 GO:0007016 GO:0007049 GO:0007154 GO:0007275 GO:0007399 GO:0007409 GO:0007528 GO:0008092 GO:0008104 GO:0008150 GO:0009653 GO:0009893 GO:0009986 GO:0009987 GO:0010035 GO:0010038 GO:0010256 GO:0010468 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0010649 GO:0010650 GO:0010765 GO:0010959 GO:0010960 GO:0012505 GO:0014704 GO:0014731 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016192 GO:0016323 GO:0016328 GO:0016528 GO:0016529 GO:0019222 GO:0019226 GO:0019228 GO:0022008 GO:0022402 GO:0022407 GO:0022409 GO:0022607 GO:0022898 GO:0023052 GO:0030016 GO:0030017 GO:0030018 GO:0030030 GO:0030054 GO:0030154 GO:0030155 GO:0030182 GO:0030315 GO:0030424 GO:0030425 GO:0030507 GO:0030674 GO:0031175 GO:0031594 GO:0031674 GO:0032026 GO:0032386 GO:0032388 GO:0032409 GO:0032410 GO:0032411 GO:0032412 GO:0032413 GO:0032414 GO:0032501 GO:0032502 GO:0032507 GO:0032879 GO:0032880 GO:0032989 GO:0032990 GO:0033036 GO:0033157 GO:0033267 GO:0033268 GO:0034110 GO:0034112 GO:0034613 GO:0034762 GO:0034763 GO:0034764 GO:0034765 GO:0034766 GO:0034767 GO:0035637 GO:0036477 GO:0042221 GO:0042383 GO:0042391 GO:0042592 GO:0042886 GO:0042995 GO:0043001 GO:0043005 GO:0043034 GO:0043194 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043266 GO:0043267 GO:0043269 GO:0043270 GO:0043271 GO:0043292 GO:0044085 GO:0044091 GO:0044092 GO:0044093 GO:0044291 GO:0044304 GO:0044325 GO:0044422 GO:0044424 GO:0044425 GO:0044444 GO:0044449 GO:0044456 GO:0044459 GO:0044463 GO:0044464 GO:0045184 GO:0045185 GO:0045202 GO:0045211 GO:0045296 GO:0045760 GO:0045785 GO:0045838 GO:0046907 GO:0048193 GO:0048468 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048666 GO:0048667 GO:0048699 GO:0048731 GO:0048812 GO:0048856 GO:0048858 GO:0048869 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050808 GO:0050839 GO:0050877 GO:0050896 GO:0051049 GO:0051050 GO:0051051 GO:0051179 GO:0051222 GO:0051223 GO:0051234 GO:0051235 GO:0051301 GO:0051641 GO:0051649 GO:0051651 GO:0051716 GO:0055065 GO:0055080 GO:0060090 GO:0060255 GO:0060341 GO:0061024 GO:0061564 GO:0061640 GO:0061951 GO:0065007 GO:0065008 GO:0065009 GO:0070201 GO:0070727 GO:0070887 GO:0071241 GO:0071248 GO:0071286 GO:0071702 GO:0071705 GO:0071709 GO:0071840 GO:0071944 GO:0072507 GO:0072657 GO:0072658 GO:0072659 GO:0072660 GO:0090087 GO:0090150 GO:0090313 GO:0090314 GO:0090316 GO:0097060 GO:0097447 GO:0097458 GO:0098590 GO:0098771 GO:0098794 GO:0098876 GO:0098900 GO:0098901 GO:0098902 GO:0099080 GO:0099081 GO:0099512 GO:0120025 GO:0120036 GO:0120038 GO:0120039 GO:1900825 GO:1900827 GO:1901016 GO:1901017 GO:1901379 GO:1901380 GO:1902259 GO:1902260 GO:1902305 GO:1902307 GO:1903047 GO:1903533 GO:1903817 GO:1903827 GO:1903829 GO:1904062 GO:1904063 GO:1904064 GO:1904181 GO:1904951 GO:1905475 GO:1905477 GO:1990778 GO:2000649 GO:2000651 GO:2001257 GO:2001258 GO:2001259
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_3 Ank_5

Protein Analysis

594

Amino Acids

63.23

Weight (kDa)

8.44

Isoelectric Point (pI)

32.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 127 - 210 5.5e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 183 - 241 1.8e-06 Ankyrin repeats (3 copies)
Ank_2 PF12796 285 - 345 1.3e-07 Ankyrin repeats (3 copies)
Ank_2 PF12796 288 - 368 1.1e-08 Ankyrin repeats (3 copies)
Ank_4 PF13637 289 - 338 9.3e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 341 - 415 3.5e-07 Ankyrin repeats (3 copies)
Ank_2 PF12796 389 - 462 1e-13 Ankyrin repeats (3 copies)
Ank_4 PF13637 389 - 438 5.6e-09 Ankyrin repeats (many copies)
Ank_5 PF13857 410 - 458 1.6e-06 Ankyrin repeats (many copies)
Ank PF00023 417 - 448 1.5e-07 Ankyrin repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 196
Acc65I GGTACC 1 cut(s) 536
AccB1I GGYRCC 2 cut(s) 500, 536
AccB7I CCANNNNNTGG 1 cut(s) 345
AccBSI CCGCTC 2 cut(s) 1264, 1411
AccII CGCG 1 cut(s) 1459
AccIII TCCGGA 1 cut(s) 412
AcoI YGGCCR 1 cut(s) 960
AcsI RAATTY 1 cut(s) 923
AdeI CACNNNGTG 1 cut(s) 1184
AfaI GTAC 2 cut(s) 219, 538
AflII CTTAAG 1 cut(s) 490
AgsI TTSAA 7 cut(s) 137, 368, 593, 901, 982, 995, 1396
AjnI CCWGG 2 cut(s) 344, 761
Alw21I GWGCWC 3 cut(s) 181, 489, 1539
Alw26I GTCTC 2 cut(s) 1049, 1298
Alw44I GTGCAC 1 cut(s) 177
AlwNI CAGNNNCTG 2 cut(s) 476, 1025
Aor13HI TCCGGA 1 cut(s) 412
AoxI GGCC 7 cut(s) 3, 20, 234, 403, 894, 960, 1592
ApaLI GTGCAC 1 cut(s) 177
ApoI RAATTY 1 cut(s) 923
Asp700I GAANNNNTTC 1 cut(s) 303
Asp718I GGTACC 1 cut(s) 536
AspLEI GCGC 2 cut(s) 1459, 1715
AspS9I GGNCC 4 cut(s) 21, 208, 283, 1592
AsuC2I CCSGG 2 cut(s) 1577, 1647
AsuHPI GGTGA 7 cut(s) 507, 653, 1064, 1426, 1675, 1718, 1738
AsuNHI GCTAGC 1 cut(s) 1429
AvaII GGWCC 2 cut(s) 208, 283
BaeGI GKGCMC 2 cut(s) 181, 990
BanI GGYRCC 2 cut(s) 500, 536
Bbv12I GWGCWC 3 cut(s) 181, 489, 1539
BccI CCATC 2 cut(s) 1244, 1510
BceAI ACGGC 3 cut(s) 947, 975, 1167
BciT130I CCWGG 2 cut(s) 346, 763
BciVI GTATCC 1 cut(s) 409
BclI TGATCA 1 cut(s) 969
BcnI CCSGG 2 cut(s) 1577, 1647
BcoDI GTCTC 2 cut(s) 1049, 1298
BfaI CTAG 7 cut(s) 771, 1127, 1278, 1421, 1430, 1448, 1596
BfmI CTRYAG 5 cut(s) 58, 172, 189, 230, 1527
BfoI RGCGCY 1 cut(s) 1716
BfrI CTTAAG 1 cut(s) 490
BfuAI ACCTGC 1 cut(s) 196
BfuI GTATCC 1 cut(s) 409
Bme1390I CCNGG 4 cut(s) 346, 763, 1577, 1647
Bme18I GGWCC 2 cut(s) 208, 283
BmgT120I GGNCC 4 cut(s) 21, 208, 283, 1592
BmiI GGNNCC 8 cut(s) 23, 210, 284, 285, 330, 502, 538, 1593
BmrFI CCNGG 4 cut(s) 346, 763, 1577, 1647
BmsI GCATC 5 cut(s) 97, 117, 816, 1186, 1624
BmtI GCTAGC 1 cut(s) 1433
BoxI GACNNNNGTC 1 cut(s) 251
BpmI CTGGAG 1 cut(s) 1170
BpuEI CTTGAG 1 cut(s) 861
BpuMI CCSGG 2 cut(s) 1577, 1647
Bsa29I ATCGAT 1 cut(s) 933
BsaI GGTCTC 1 cut(s) 1298
BsaJI CCNNGG 5 cut(s) 400, 571, 762, 1183, 1749
BsaWI WCCGGW 2 cut(s) 412, 1557
Bse118I RCCGGY 2 cut(s) 236, 881
Bse1I ACTGG 1 cut(s) 535
BseAI TCCGGA 1 cut(s) 412
BseBI CCWGG 2 cut(s) 346, 763
BseCI ATCGAT 1 cut(s) 933
BseDI CCNNGG 5 cut(s) 400, 571, 762, 1183, 1749
BseGI GGATG 7 cut(s) 112, 132, 256, 617, 1195, 1201, 1521
BseMII CTCAG 1 cut(s) 680
BseNI ACTGG 1 cut(s) 535
BseRI GAGGAG 2 cut(s) 386, 1636
BseSI GKGCMC 2 cut(s) 181, 990
BseX3I CGGCCG 1 cut(s) 960
BseYI CCCAGC 1 cut(s) 1542
BsgI GTGCAG 5 cut(s) 135, 372, 428, 646, 1194
Bsh1236I CGCG 1 cut(s) 1459
Bsh1285I CGRYCG 1 cut(s) 963
BshFI GGCC 7 cut(s) 5, 22, 236, 405, 896, 962, 1594
BshNI GGYRCC 2 cut(s) 500, 536
BshVI ATCGAT 1 cut(s) 933
BsiEI CGRYCG 1 cut(s) 963
BsiHKAI GWGCWC 3 cut(s) 181, 489, 1539
BsiSI CCGG 6 cut(s) 237, 413, 882, 1558, 1576, 1647
BsiWI CGTACG 1 cut(s) 217
BslFI GGGAC 3 cut(s) 194, 296, 1223
BsmAI GTCTC 2 cut(s) 1049, 1298
BsmFI GGGAC 3 cut(s) 194, 296, 1223
BsmI GAATGC 2 cut(s) 998, 1348
BsnI GGCC 7 cut(s) 5, 22, 236, 405, 896, 962, 1594
Bso31I GGTCTC 1 cut(s) 1298
Bsp1286I GDGCHC 4 cut(s) 181, 489, 990, 1539
Bsp13I TCCGGA 1 cut(s) 412
Bsp143I GATC 2 cut(s) 657, 969
BspANI GGCC 7 cut(s) 5, 22, 236, 405, 896, 962, 1594
BspCNI CTCAG 1 cut(s) 681
BspDI ATCGAT 1 cut(s) 933
BspEI TCCGGA 1 cut(s) 412
BspFNI CGCG 1 cut(s) 1459
BspHI TCATGA 2 cut(s) 90, 1778
BspLI GGNNCC 8 cut(s) 23, 210, 284, 285, 330, 502, 538, 1593
BspMAI CTGCAG 2 cut(s) 62, 193
BspMI ACCTGC 1 cut(s) 196
BspOI GCTAGC 1 cut(s) 1433
BspQI GCTCTTC 1 cut(s) 159
BspT107I GGYRCC 2 cut(s) 500, 536
BspTI CTTAAG 1 cut(s) 490
BspTNI GGTCTC 1 cut(s) 1298
BsrBI CCGCTC 2 cut(s) 1264, 1411
BsrFI RCCGGY 2 cut(s) 236, 881
BsrI ACTGG 1 cut(s) 535
BssAI RCCGGY 2 cut(s) 236, 881
BssECI CCNNGG 5 cut(s) 400, 571, 762, 1183, 1749
BssMI GATC 2 cut(s) 657, 969
BssT1I CCWWGG 3 cut(s) 400, 571, 1749
Bst2UI CCWGG 2 cut(s) 346, 763
Bst4CI ACNGT 5 cut(s) 173, 1038, 1091, 1292, 1528
Bst6I CTCTTC 2 cut(s) 159, 294
BstAFI CTTAAG 1 cut(s) 490
BstAPI GCANNNNNTGC 1 cut(s) 710
BstDEI CTNAG 2 cut(s) 144, 689
BstDSI CCRYGG 1 cut(s) 1183
BstENI CCTNNNNNAGG 1 cut(s) 1609
BstF5I GGATG 7 cut(s) 112, 132, 256, 617, 1195, 1201, 1521
BstFNI CGCG 1 cut(s) 1459
BstH2I RGCGCY 1 cut(s) 1716
BstHHI GCGC 2 cut(s) 1459, 1715
BstKTI GATC 2 cut(s) 660, 972
BstMAI GTCTC 2 cut(s) 1049, 1298
BstMBI GATC 2 cut(s) 657, 969
BstMCI CGRYCG 1 cut(s) 963
BstMWI GCNNNNNNNGC 9 cut(s) 38, 116, 185, 323, 710, 767, 878, 902, 1710
BstNI CCWGG 2 cut(s) 346, 763
BstNSI RCATGY 2 cut(s) 41, 394
BstPAI GACNNNNGTC 1 cut(s) 251
BstSCI CCNGG 4 cut(s) 344, 761, 1575, 1645
BstSFI CTRYAG 5 cut(s) 58, 172, 189, 230, 1527
BstSLI GKGCMC 2 cut(s) 181, 990
BstUI CGCG 1 cut(s) 1459
BstZI CGGCCG 1 cut(s) 960
Bsu15I ATCGAT 1 cut(s) 933
BsuI GTATCC 1 cut(s) 409
BsuRI GGCC 7 cut(s) 5, 22, 236, 405, 896, 962, 1594
BsuTUI ATCGAT 1 cut(s) 933
BtgI CCRYGG 1 cut(s) 1183
BtgZI GCGATG 1 cut(s) 1647
BtsCI GGATG 7 cut(s) 112, 132, 256, 617, 1195, 1201, 1521
BtsI GCAGTG 2 cut(s) 360, 972
BtsIMutI CAGTG 2 cut(s) 360, 972
BveI ACCTGC 1 cut(s) 196
CaiI CAGNNNCTG 2 cut(s) 476, 1025
CciI TCATGA 2 cut(s) 90, 1778
CfoI GCGC 2 cut(s) 1459, 1715
Cfr10I RCCGGY 2 cut(s) 236, 881
Cfr13I GGNCC 4 cut(s) 21, 208, 283, 1592
ClaI ATCGAT 1 cut(s) 933
CsiI ACCWGGT 1 cut(s) 344
Csp6I GTAC 2 cut(s) 218, 537
CviQI GTAC 2 cut(s) 218, 537
DdeI CTNAG 2 cut(s) 144, 689
DpnI GATC 2 cut(s) 659, 971
DpnII GATC 2 cut(s) 657, 969
DraIII CACNNNGTG 1 cut(s) 1184
EaeI YGGCCR 1 cut(s) 960
EagI CGGCCG 1 cut(s) 960
Eam1104I CTCTTC 2 cut(s) 159, 294
EarI CTCTTC 2 cut(s) 159, 294
EciI GGCGGA 3 cut(s) 341, 834, 1598
EclXI CGGCCG 1 cut(s) 960
Eco130I CCWWGG 3 cut(s) 400, 571, 1749
Eco147I AGGCCT 2 cut(s) 405, 896
Eco31I GGTCTC 1 cut(s) 1298
Eco32I GATATC 2 cut(s) 1314, 1336
Eco47I GGWCC 2 cut(s) 208, 283
Eco52I CGGCCG 1 cut(s) 960
EcoNI CCTNNNNNAGG 1 cut(s) 1609
EcoO109I RGGNCCY 3 cut(s) 208, 283, 1592
EcoRII CCWGG 2 cut(s) 344, 761
EcoRV GATATC 2 cut(s) 1314, 1336
EcoT14I CCWWGG 3 cut(s) 400, 571, 1749
ErhI CCWWGG 3 cut(s) 400, 571, 1749
FaqI GGGAC 3 cut(s) 194, 296, 1223
FauI CCCGC 2 cut(s) 575, 1404
FbaI TGATCA 1 cut(s) 969
FokI GGATG 7 cut(s) 119, 139, 263, 624, 1202, 1208, 1528
FspBI CTAG 7 cut(s) 771, 1127, 1278, 1421, 1430, 1448, 1596
GlaI GCGC 2 cut(s) 1458, 1714
GsaI CCCAGC 1 cut(s) 1546
GsuI CTGGAG 1 cut(s) 1170
HaeII RGCGCY 1 cut(s) 1716
HaeIII GGCC 7 cut(s) 5, 22, 236, 405, 896, 962, 1594
HapII CCGG 6 cut(s) 237, 413, 882, 1558, 1576, 1647
HhaI GCGC 2 cut(s) 1459, 1715
Hin6I GCGC 2 cut(s) 1457, 1713
HinP1I GCGC 2 cut(s) 1457, 1713
HincII GTYRAC 2 cut(s) 169, 1212
HindII GTYRAC 2 cut(s) 169, 1212
HindIII AAGCTT 1 cut(s) 1739
HinfI GANTC 4 cut(s) 16, 512, 605, 635
HpaI GTTAAC 1 cut(s) 1212
HpaII CCGG 6 cut(s) 237, 413, 882, 1558, 1576, 1647
HphI GGTGA 7 cut(s) 507, 653, 1064, 1426, 1675, 1718, 1738
Hpy166II GTNNAC 5 cut(s) 82, 169, 179, 1212, 1304
Hpy188I TCNGA 5 cut(s) 10, 552, 690, 1075, 1772
Hpy188III TCNNGA 9 cut(s) 91, 413, 609, 918, 941, 1309, 1339, 1465, 1779
Hpy8I GTNNAC 5 cut(s) 82, 169, 179, 1212, 1304
HpyAV CCTTC 5 cut(s) 976, 1099, 1277, 1471, 1694
HpyCH4III ACNGT 5 cut(s) 173, 1038, 1091, 1292, 1528
HpyCH4IV ACGT 2 cut(s) 220, 990
HpyF10VI GCNNNNNNNGC 9 cut(s) 38, 116, 185, 323, 710, 767, 878, 902, 1710
HpyF3I CTNAG 2 cut(s) 144, 689
HpySE526I ACGT 2 cut(s) 220, 990
HspAI GCGC 2 cut(s) 1457, 1713
KflI GGGWCCC 1 cut(s) 283
Kpn2I TCCGGA 1 cut(s) 412
KpnI GGTACC 1 cut(s) 540
KroI GCCGGC 1 cut(s) 881
KroNI GCCGGC 1 cut(s) 883
Ksp22I TGATCA 1 cut(s) 969
KspAI GTTAAC 1 cut(s) 1212
Kzo9I GATC 2 cut(s) 657, 969
LguI GCTCTTC 1 cut(s) 159
LmnI GCTCC 6 cut(s) 269, 328, 399, 473, 1623, 1772
LweI GCATC 5 cut(s) 97, 117, 816, 1186, 1624
MabI ACCWGGT 1 cut(s) 344
MaeI CTAG 7 cut(s) 771, 1127, 1278, 1421, 1430, 1448, 1596
MaeII ACGT 2 cut(s) 220, 990
MaeIII GTNAC 9 cut(s) 46, 777, 1040, 1091, 1142, 1253, 1286, 1663, 1744
MalI GATC 2 cut(s) 659, 971
MbiI CCGCTC 2 cut(s) 1264, 1411
MboI GATC 2 cut(s) 657, 969
MboII GAAGA 4 cut(s) 146, 311, 444, 692
MhlI GDGCHC 4 cut(s) 181, 489, 990, 1539
MluCI AATT 4 cut(s) 864, 923, 1062, 1205
MroI TCCGGA 1 cut(s) 412
MroNI GCCGGC 1 cut(s) 881
MroXI GAANNNNTTC 1 cut(s) 303
MseI TTAA 6 cut(s) 491, 522, 546, 599, 729, 1211
MslI CAYNNNNRTG 3 cut(s) 89, 162, 277
MspA1I CMGCKG 4 cut(s) 57, 119, 650, 707
MspCI CTTAAG 1 cut(s) 490
MspI CCGG 6 cut(s) 237, 413, 882, 1558, 1576, 1647
MspR9I CCNGG 4 cut(s) 346, 763, 1577, 1647
Mva1269I GAATGC 2 cut(s) 998, 1348
MvaI CCWGG 2 cut(s) 346, 763
MvnI CGCG 1 cut(s) 1459
MwoI GCNNNNNNNGC 9 cut(s) 38, 116, 185, 323, 710, 767, 878, 902, 1710
NaeI GCCGGC 1 cut(s) 883
NciI CCSGG 2 cut(s) 1577, 1647
NdeII GATC 2 cut(s) 657, 969
NgoMIV GCCGGC 1 cut(s) 881
NheI GCTAGC 1 cut(s) 1429
NlaIV GGNNCC 8 cut(s) 23, 210, 284, 285, 330, 502, 538, 1593
NmeAIII GCCGAG 1 cut(s) 1741
NmuCI GTSAC 5 cut(s) 46, 777, 1091, 1663, 1744
NspI RCATGY 2 cut(s) 41, 394
PaeI GCATGC 1 cut(s) 41
PagI TCATGA 2 cut(s) 90, 1778
PceI AGGCCT 2 cut(s) 405, 896
PciSI GCTCTTC 1 cut(s) 159
PctI GAATGC 2 cut(s) 998, 1348
PdiI GCCGGC 1 cut(s) 883
PdmI GAANNNNTTC 1 cut(s) 303
PfeI GAWTC 4 cut(s) 16, 512, 605, 635
Pfl23II CGTACG 1 cut(s) 217
PflMI CCANNNNNTGG 1 cut(s) 345
PpuMI RGGWCCY 2 cut(s) 208, 283
PshAI GACNNNNGTC 1 cut(s) 251
Psp5II RGGWCCY 2 cut(s) 208, 283
Psp6I CCWGG 2 cut(s) 344, 761
PspFI CCCAGC 1 cut(s) 1542
PspGI CCWGG 2 cut(s) 344, 761
PspLI CGTACG 1 cut(s) 217
PspN4I GGNNCC 8 cut(s) 23, 210, 284, 285, 330, 502, 538, 1593
PspPI GGNCC 4 cut(s) 21, 208, 283, 1592
PspPPI RGGWCCY 2 cut(s) 208, 283
PstI CTGCAG 2 cut(s) 62, 193
PstNI CAGNNNCTG 2 cut(s) 476, 1025
PvuII CAGCTG 4 cut(s) 57, 119, 650, 707
RsaI GTAC 2 cut(s) 219, 538
RsaNI GTAC 2 cut(s) 218, 537
RseI CAYNNNNRTG 3 cut(s) 89, 162, 277
SapI GCTCTTC 1 cut(s) 159
SaqAI TTAA 6 cut(s) 491, 522, 546, 599, 729, 1211
Sau3AI GATC 2 cut(s) 657, 969
Sau96I GGNCC 4 cut(s) 21, 208, 283, 1592
ScrFI CCNGG 4 cut(s) 346, 763, 1577, 1647
SduI GDGCHC 4 cut(s) 181, 489, 990, 1539
SexAI ACCWGGT 1 cut(s) 344
SfaNI GCATC 5 cut(s) 97, 117, 816, 1186, 1624
SfcI CTRYAG 5 cut(s) 58, 172, 189, 230, 1527
SinI GGWCC 2 cut(s) 208, 283
SmiMI CAYNNNNRTG 3 cut(s) 89, 162, 277
SmlI CTYRAG 2 cut(s) 490, 876
SmoI CTYRAG 2 cut(s) 490, 876
SphI GCATGC 1 cut(s) 41
Sse9I AATT 4 cut(s) 864, 923, 1062, 1205
SseBI AGGCCT 2 cut(s) 405, 896
SspI AATATT 1 cut(s) 889
SspMI CTAG 7 cut(s) 771, 1127, 1278, 1421, 1430, 1448, 1596
StuI AGGCCT 2 cut(s) 405, 896
StyD4I CCNGG 4 cut(s) 344, 761, 1575, 1645
StyI CCWWGG 3 cut(s) 400, 571, 1749
TaaI ACNGT 5 cut(s) 173, 1038, 1091, 1292, 1528
TaiI ACGT 2 cut(s) 223, 993
TaqI TCGA 3 cut(s) 755, 933, 1116
TasI AATT 4 cut(s) 864, 923, 1062, 1205
TauI GCSGC 4 cut(s) 704, 737, 1277, 1386
TfiI GAWTC 4 cut(s) 16, 512, 605, 635
Tru1I TTAA 6 cut(s) 491, 522, 546, 599, 729, 1211
Tru9I TTAA 6 cut(s) 491, 522, 546, 599, 729, 1211
TscAI CASTG 2 cut(s) 360, 979
TseFI GTSAC 5 cut(s) 46, 777, 1091, 1663, 1744
Tsp45I GTSAC 5 cut(s) 46, 777, 1091, 1663, 1744
TspDTI ATGAA 3 cut(s) 107, 635, 1656
TspGWI ACGGA 3 cut(s) 263, 470, 1260
TspRI CASTG 2 cut(s) 360, 979
Van91I CCANNNNNTGG 1 cut(s) 345
Vha464I CTTAAG 1 cut(s) 490
VneI GTGCAC 1 cut(s) 177
VpaK11BI GGWCC 2 cut(s) 208, 283
XagI CCTNNNNNAGG 1 cut(s) 1609
XapI RAATTY 1 cut(s) 923
XceI RCATGY 2 cut(s) 41, 394
XmnI GAANNNNTTC 1 cut(s) 303
XspI CTAG 7 cut(s) 771, 1127, 1278, 1421, 1430, 1448, 1596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.