pycom17g09080

APO protein 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0002143
Physical Location & Seq
Reverse (-)
6799514 .. 6800383
870 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 645 bp
ATGATAATTAATGAAAATTTGAAGTCTATATTGTGTACAGACGATATTCTAAACTACTCTAATTTATGGGACGGTGATTCGAACTGTGGTGCAAGGGATGGAACTGTTGGAAGGTGTTTTTTTTTTTTGGATGCAAAGGTTTTGGATCCAATAAAGAGTTCTGCTTTGGTGGTTAATGTAGTTGCTTTCAGTTCCCAAGTGTTGTACTTATTGATATGTTTTCTGATCGACTCATTGGGGTTTTGTTCACACTGTGGAGAAGACGTGATTTTGTGTAAAAATTGGAACCAAAAGGAAATTATGGCGCTGAGGAAGAAACTGTGGGAGAGTTCCGATTTCAATGGAAAATTTGTGTACAGCTTTTCGAGGTATTACAGTTCAAACAAGGTGGACCTAAGGAAGCTAAAGCCTAGGATTCAGAATAAAATCCAAAACCGGACTAAGGATTACCCAATGCCGGCCATGATACCGGTGGCCCAGGAGGTCATGGAGTGCCAGAGACTCGTTATGCAAGGTGTCTCTACTCTCCTCAAAATGGTGCCCGTTCTGGCCTGCAAGTTCTGTCCAGAAGTATATATAGGCGAGAAATGCCACTTAATTCAGACTTGTTGTGGCTTTAAACGTCGTGGCAAGAATAAAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
APO_RNA-bind

Protein Analysis

215

Amino Acids

24.42

Weight (kDa)

8.93

Isoelectric Point (pI)

39.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
APO_RNA-bind PF05634 128 - 208 2e-14 APO RNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 538
AclWI GGATC 2 cut(s) 140, 153
AcoI YGGCCR 1 cut(s) 459
AcsI RAATTY 2 cut(s) 16, 347
AdeI CACNNNGTG 1 cut(s) 254
AfaI GTAC 3 cut(s) 37, 206, 356
AfiI CCNNNNNNNGG 3 cut(s) 442, 457, 535
AgeI ACCGGT 1 cut(s) 469
AgsI TTSAA 3 cut(s) 22, 340, 381
AjiI CACGTC 1 cut(s) 265
AjnI CCWGG 1 cut(s) 477
AjuI GAANNNNNNNTTGG 2 cut(s) 142, 174
AluBI AGCT 2 cut(s) 360, 403
AluI AGCT 2 cut(s) 360, 403
Alw26I GTCTC 2 cut(s) 493, 523
AlwI GGATC 2 cut(s) 140, 153
AoxI GGCC 3 cut(s) 459, 474, 549
ApoI RAATTY 2 cut(s) 16, 347
AseI ATTAAT 1 cut(s) 9
AsiGI ACCGGT 1 cut(s) 469
AspA2I CCTAGG 1 cut(s) 410
AspLEI GCGC 1 cut(s) 307
AspS9I GGNCC 2 cut(s) 391, 475
AsuHPI GGTGA 1 cut(s) 86
AsuII TTCGAA 1 cut(s) 80
AvaII GGWCC 1 cut(s) 391
AvrII CCTAGG 1 cut(s) 410
AxyI CCTNAGG 1 cut(s) 395
BaeGI GKGCMC 1 cut(s) 543
BamHI GGATCC 1 cut(s) 145
BanI GGYRCC 1 cut(s) 538
BbsI GAAGAC 1 cut(s) 267
BbvCI CCTCAGC 1 cut(s) 308
BccI CCATC 1 cut(s) 92
BciT130I CCWGG 1 cut(s) 479
BcoDI GTCTC 2 cut(s) 493, 523
BfaI CTAG 1 cut(s) 411
BfoI RGCGCY 1 cut(s) 308
BlnI CCTAGG 1 cut(s) 410
Bme1390I CCNGG 1 cut(s) 479
Bme18I GGWCC 1 cut(s) 391
BmgBI CACGTC 1 cut(s) 265
BmgT120I GGNCC 2 cut(s) 391, 475
BmiI GGNNCC 3 cut(s) 147, 287, 540
BmrFI CCNGG 1 cut(s) 479
BmsI GCATC 1 cut(s) 121
BpiI GAAGAC 1 cut(s) 267
Bpu10I CCTNAGC 1 cut(s) 308
Bpu14I TTCGAA 1 cut(s) 80
BsaJI CCNNGG 2 cut(s) 410, 477
BsaWI WCCGGW 2 cut(s) 435, 469
BsaXI ACNNNNNCTCC 2 cut(s) 249, 279
Bsc4I CCNNNNNNNGG 3 cut(s) 442, 457, 535
Bse118I RCCGGY 2 cut(s) 457, 469
Bse21I CCTNAGG 1 cut(s) 395
BseBI CCWGG 1 cut(s) 479
BseDI CCNNGG 2 cut(s) 410, 477
BseGI GGATG 2 cut(s) 103, 136
BseLI CCNNNNNNNGG 3 cut(s) 442, 457, 535
BseMII CTCAG 1 cut(s) 299
BseRI GAGGAG 1 cut(s) 518
BseSI GKGCMC 1 cut(s) 543
BshFI GGCC 3 cut(s) 461, 476, 551
BshNI GGYRCC 1 cut(s) 538
BshTI ACCGGT 1 cut(s) 469
BsiSI CCGG 3 cut(s) 436, 458, 470
BslFI GGGAC 1 cut(s) 83
BslI CCNNNNNNNGG 3 cut(s) 442, 457, 535
BsmAI GTCTC 2 cut(s) 493, 523
BsmFI GGGAC 1 cut(s) 83
BsnI GGCC 3 cut(s) 461, 476, 551
Bsp119I TTCGAA 1 cut(s) 80
Bsp1286I GDGCHC 1 cut(s) 543
Bsp1407I TGTACA 2 cut(s) 35, 354
Bsp143I GATC 2 cut(s) 145, 225
BspANI GGCC 3 cut(s) 461, 476, 551
BspCNI CTCAG 1 cut(s) 300
BspLI GGNNCC 3 cut(s) 147, 287, 540
BspPI GGATC 2 cut(s) 140, 153
BspT104I TTCGAA 1 cut(s) 80
BspT107I GGYRCC 1 cut(s) 538
BsrFI RCCGGY 2 cut(s) 457, 469
BsrGI TGTACA 2 cut(s) 35, 354
BssAI RCCGGY 2 cut(s) 457, 469
BssECI CCNNGG 2 cut(s) 410, 477
BssMI GATC 2 cut(s) 145, 225
BssT1I CCWWGG 1 cut(s) 410
Bst2UI CCWGG 1 cut(s) 479
Bst4CI ACNGT 6 cut(s) 74, 86, 106, 254, 321, 377
BstAUI TGTACA 2 cut(s) 35, 354
BstBI TTCGAA 1 cut(s) 80
BstC8I GCNNGC 2 cut(s) 459, 553
BstDEI CTNAG 3 cut(s) 308, 395, 441
BstF5I GGATG 2 cut(s) 103, 136
BstH2I RGCGCY 1 cut(s) 308
BstHHI GCGC 1 cut(s) 307
BstKTI GATC 2 cut(s) 148, 228
BstMAI GTCTC 2 cut(s) 493, 523
BstMBI GATC 2 cut(s) 145, 225
BstMWI GCNNNNNNNGC 1 cut(s) 588
BstNI CCWGG 1 cut(s) 479
BstSCI CCNGG 1 cut(s) 477
BstSLI GKGCMC 1 cut(s) 543
BstV2I GAAGAC 1 cut(s) 267
BstX2I RGATCY 1 cut(s) 145
BstYI RGATCY 1 cut(s) 145
Bsu36I CCTNAGG 1 cut(s) 395
BsuRI GGCC 3 cut(s) 461, 476, 551
BtrI CACGTC 1 cut(s) 265
BtsCI GGATG 2 cut(s) 103, 136
BtsIMutI CAGTG 1 cut(s) 250
Cac8I GCNNGC 2 cut(s) 459, 553
CfoI GCGC 1 cut(s) 307
Cfr10I RCCGGY 2 cut(s) 457, 469
Cfr13I GGNCC 2 cut(s) 391, 475
Csp6I GTAC 3 cut(s) 36, 205, 355
CspAI ACCGGT 1 cut(s) 469
CspCI CAANNNNNGTGG 2 cut(s) 369, 404
CviAII CATG 2 cut(s) 463, 487
CviJI RGCY 7 cut(s) 360, 403, 409, 461, 476, 551, 615
CviKI_1 RGCY 7 cut(s) 360, 403, 409, 461, 476, 551, 615
CviQI GTAC 3 cut(s) 36, 205, 355
DdeI CTNAG 3 cut(s) 308, 395, 441
DpnI GATC 2 cut(s) 147, 227
DpnII GATC 2 cut(s) 145, 225
DraI TTTAAA 1 cut(s) 619
DraIII CACNNNGTG 1 cut(s) 254
EaeI YGGCCR 1 cut(s) 459
Eco130I CCWWGG 1 cut(s) 410
Eco47I GGWCC 1 cut(s) 391
Eco81I CCTNAGG 1 cut(s) 395
EcoRII CCWGG 1 cut(s) 477
EcoT14I CCWWGG 1 cut(s) 410
ErhI CCWWGG 1 cut(s) 410
FaeI CATG 2 cut(s) 466, 490
FaqI GGGAC 1 cut(s) 83
FatI CATG 2 cut(s) 462, 486
FokI GGATG 2 cut(s) 110, 143
FspBI CTAG 1 cut(s) 411
GlaI GCGC 1 cut(s) 306
HaeII RGCGCY 1 cut(s) 308
HaeIII GGCC 3 cut(s) 461, 476, 551
HapII CCGG 3 cut(s) 436, 458, 470
HhaI GCGC 1 cut(s) 307
Hin1II CATG 2 cut(s) 466, 490
Hin6I GCGC 1 cut(s) 305
HinP1I GCGC 1 cut(s) 305
HinfI GANTC 4 cut(s) 77, 230, 415, 501
HpaII CCGG 3 cut(s) 436, 458, 470
HphI GGTGA 1 cut(s) 86
Hpy166II GTNNAC 4 cut(s) 36, 248, 355, 391
Hpy188I TCNGA 4 cut(s) 225, 334, 420, 603
Hpy188III TCNNGA 1 cut(s) 566
Hpy8I GTNNAC 4 cut(s) 36, 248, 355, 391
Hpy99I CGWCG 1 cut(s) 627
HpyAV CCTTC 1 cut(s) 105
HpyCH4III ACNGT 6 cut(s) 74, 86, 106, 254, 321, 377
HpyCH4IV ACGT 2 cut(s) 264, 622
HpyCH4V TGCA 4 cut(s) 92, 134, 511, 555
HpyF10VI GCNNNNNNNGC 1 cut(s) 588
HpyF3I CTNAG 3 cut(s) 308, 395, 441
HpySE526I ACGT 2 cut(s) 264, 622
Hsp92II CATG 2 cut(s) 466, 490
HspAI GCGC 1 cut(s) 305
KroI GCCGGC 1 cut(s) 457
KroNI GCCGGC 1 cut(s) 459
Kzo9I GATC 2 cut(s) 145, 225
LpnPI CCDG 9 cut(s) 449, 464, 471, 483, 491, 509, 533, 565, 579
LweI GCATC 1 cut(s) 121
MaeI CTAG 1 cut(s) 411
MaeII ACGT 2 cut(s) 264, 622
MalI GATC 2 cut(s) 147, 227
MboI GATC 2 cut(s) 145, 225
MboII GAAGA 2 cut(s) 272, 325
MflI RGATCY 1 cut(s) 145
MhlI GDGCHC 1 cut(s) 543
MluCI AATT 7 cut(s) 6, 16, 61, 280, 297, 347, 597
MlyI GAGTC 2 cut(s) 224, 495
MmeI TCCRAC 1 cut(s) 88
MnlI CCTC 4 cut(s) 303, 360, 475, 539
MroNI GCCGGC 1 cut(s) 457
MseI TTAA 4 cut(s) 9, 174, 596, 618
MspI CCGG 3 cut(s) 436, 458, 470
MspR9I CCNGG 1 cut(s) 479
MvaI CCWGG 1 cut(s) 479
MwoI GCNNNNNNNGC 1 cut(s) 588
NaeI GCCGGC 1 cut(s) 459
NdeII GATC 2 cut(s) 145, 225
NgoMIV GCCGGC 1 cut(s) 457
NlaIII CATG 2 cut(s) 466, 490
NlaIV GGNNCC 3 cut(s) 147, 287, 540
NspV TTCGAA 1 cut(s) 80
PdiI GCCGGC 1 cut(s) 459
PfeI GAWTC 2 cut(s) 77, 415
PinAI ACCGGT 1 cut(s) 469
PleI GAGTC 2 cut(s) 224, 495
PpsI GAGTC 2 cut(s) 224, 495
PshBI ATTAAT 1 cut(s) 9
Psp6I CCWGG 1 cut(s) 477
PspGI CCWGG 1 cut(s) 477
PspN4I GGNNCC 3 cut(s) 147, 287, 540
PspPI GGNCC 2 cut(s) 391, 475
PsuI RGATCY 1 cut(s) 145
RsaI GTAC 3 cut(s) 37, 206, 356
RsaNI GTAC 3 cut(s) 36, 205, 355
SaqAI TTAA 4 cut(s) 9, 174, 596, 618
Sau3AI GATC 2 cut(s) 145, 225
Sau96I GGNCC 2 cut(s) 391, 475
SchI GAGTC 2 cut(s) 224, 495
ScrFI CCNGG 1 cut(s) 479
SduI GDGCHC 1 cut(s) 543
SfaNI GCATC 1 cut(s) 121
SfuI TTCGAA 1 cut(s) 80
SinI GGWCC 1 cut(s) 391
Sse9I AATT 7 cut(s) 6, 16, 61, 280, 297, 347, 597
SspMI CTAG 1 cut(s) 411
StyD4I CCNGG 1 cut(s) 477
StyI CCWWGG 1 cut(s) 410
TaaI ACNGT 6 cut(s) 74, 86, 106, 254, 321, 377
TaiI ACGT 2 cut(s) 267, 625
TaqI TCGA 3 cut(s) 80, 228, 365
TasI AATT 7 cut(s) 6, 16, 61, 280, 297, 347, 597
TatI WGTACW 3 cut(s) 35, 204, 354
TfiI GAWTC 2 cut(s) 77, 415
Tru1I TTAA 4 cut(s) 9, 174, 596, 618
Tru9I TTAA 4 cut(s) 9, 174, 596, 618
TscAI CASTG 1 cut(s) 257
TspDTI ATGAA 1 cut(s) 27
TspRI CASTG 1 cut(s) 257
VpaK11BI GGWCC 1 cut(s) 391
VspI ATTAAT 1 cut(s) 9
XapI RAATTY 2 cut(s) 16, 347
XcmI CCANNNNNNNNNTGG 1 cut(s) 469
XmaJI CCTAGG 1 cut(s) 410
XspI CTAG 1 cut(s) 411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.