pycom17g18890

Inositol oxygenase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0001948
Physical Location & Seq
Forward (+)
16418904 .. 16421663
2760 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 927 bp
ATGACTATCCGCATTGAGCAGCCCGAGTTTGGAGTTGAAGTTGAAATTGAAGCAGGGGAGAGAAAGGTGTTAAATGATGAAAAGGAATTGGCTTTAGATGCTGGATTTGTGGTGCCACATACCAATTCATTTGGACAAACATTTAGGGATTATGATGCAGAAGGAGAGAGACAACCAAGTGTTGAGAATTTCTACCAGATCAATCACATCAATCAGACTTATGATTTTGTGCAGAGAATGAGAAATGAGTATAGCAAATTGAACAGAGTGGAGATGAGCATTTGGGAATGCTGTGAACTTCTCAACGAGGTTGTCGACGAGAGCGATCCCGACTTGGACGAGCCTCAAATCGAGCACTTGTTGCAAACAGCTGAAGCCCTTAGGAAGGACTATCCTAATGAAGATTGGCTGCACTTGACTGGCCTCATCCATGACCTTGGAAAAGTGCTTCTTCTTCCTAGCTTTGGGGAGCTTCCTCAATGGGCAGTTGTCGGTGATACATTCCCTGTTGGCTGTGCTTTTGACCAATCCAATGTTCATCACAAGTACTTTGCGGAAAATCCTGACTACTATAATCCTACTTACAACACTAAGTATGGAGTTTACTCGGAGGGATGTGGACTCGAAAATGCTACGATGTCATGGGGGCACGATGATTACATGTATTTGGTGGCCAAAGAGAACAAATCAACTCTGCCTTCAGCAGCTCTGTTTATCATTAGATACCACTCATTCTATGCGTTACATAAGGCGGGAGCGTATAAGCACTTAATGAACGAAGAGGATGTCGAAAACCTGAAATGGCTCCGTGTATTCAACAAGTATGACCTTTACAGCAAGAGCAAGGTACGGGTTGACGTTGAGAAGGTGAAGCCATACTATATTTCTCTCATTGAAAAGTACTTTCCTTCGAAGCTAAGATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0016491 GO:0016701 GO:0050113 GO:0055114
KEGG Pathways
Metabolic & Signaling
ko00053 ko00562 map00053 map00562
Pfam Domains
Protein Families
MIOX

Protein Analysis

309

Amino Acids

36.06

Weight (kDa)

4.94

Isoelectric Point (pI)

47.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIOX PF05153 60 - 308 5.4e-125 Myo-inositol oxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 112
AccI GTMKAC 1 cut(s) 315
AciI CCGC 3 cut(s) 10, 554, 752
AclWI GGATC 1 cut(s) 320
AcoI YGGCCR 1 cut(s) 672
AcsI RAATTY 1 cut(s) 187
AcuI CTGAAG 2 cut(s) 393, 684
AfaI GTAC 3 cut(s) 548, 849, 902
AfiI CCNNNNNNNGG 3 cut(s) 29, 385, 464
AflIII ACRYGT 1 cut(s) 660
AgsI TTSAA 6 cut(s) 38, 44, 50, 262, 817, 896
AjuI GAANNNNNNNTTGG 2 cut(s) 519, 551
AluBI AGCT 5 cut(s) 371, 462, 472, 707, 916
AluI AGCT 5 cut(s) 371, 462, 472, 707, 916
Alw21I GWGCWC 1 cut(s) 357
Alw26I GTCTC 1 cut(s) 163
AlwI GGATC 1 cut(s) 320
Ama87I CYCGRG 1 cut(s) 23
AoxI GGCC 2 cut(s) 421, 672
ApeKI GCWGC 3 cut(s) 19, 409, 704
ApoI RAATTY 1 cut(s) 187
ArsI GACNNNNNNTTYG 2 cut(s) 771, 803
AsuHPI GGTGA 2 cut(s) 506, 880
AsuII TTCGAA 1 cut(s) 911
AvaI CYCGRG 1 cut(s) 23
AxyI CCTNAGG 1 cut(s) 380
BaeGI GKGCMC 1 cut(s) 651
BalI TGGCCA 1 cut(s) 674
BanI GGYRCC 1 cut(s) 112
Bbv12I GWGCWC 1 cut(s) 357
BbvI GCAGC 3 cut(s) 31, 396, 716
BccI CCATC 1 cut(s) 915
BcoDI GTCTC 1 cut(s) 163
BfaI CTAG 1 cut(s) 459
BisI GCNGC 3 cut(s) 20, 410, 705
BlsI GCNGC 3 cut(s) 21, 411, 706
BmcAI AGTACT 2 cut(s) 548, 902
BmeT110I CYCGRG 1 cut(s) 23
BmiI GGNNCC 2 cut(s) 114, 806
BmsI GCATC 2 cut(s) 88, 145
Bpu14I TTCGAA 1 cut(s) 911
BsaJI CCNNGG 1 cut(s) 436
BsaXI ACNNNNNCTCC 2 cut(s) 24, 54
Bsc4I CCNNNNNNNGG 3 cut(s) 29, 385, 464
Bse1I ACTGG 1 cut(s) 424
Bse21I CCTNAGG 1 cut(s) 380
BseDI CCNNGG 1 cut(s) 436
BseGI GGATG 3 cut(s) 426, 620, 790
BseLI CCNNNNNNNGG 3 cut(s) 29, 385, 464
BseNI ACTGG 1 cut(s) 424
BseSI GKGCMC 1 cut(s) 651
BseXI GCAGC 3 cut(s) 31, 396, 716
BsgI GTGCAG 2 cut(s) 251, 395
BshFI GGCC 2 cut(s) 423, 674
BshNI GGYRCC 1 cut(s) 112
BsiHKAI GWGCWC 1 cut(s) 357
BsiHKCI CYCGRG 1 cut(s) 23
BslI CCNNNNNNNGG 3 cut(s) 29, 385, 464
BsmAI GTCTC 1 cut(s) 163
BsmI GAATGC 1 cut(s) 293
BsnI GGCC 2 cut(s) 423, 674
BsoBI CYCGRG 1 cut(s) 23
Bsp119I TTCGAA 1 cut(s) 911
Bsp1286I GDGCHC 2 cut(s) 357, 651
Bsp143I GATC 2 cut(s) 198, 325
BspACI CCGC 3 cut(s) 10, 554, 752
BspANI GGCC 2 cut(s) 423, 674
BspLI GGNNCC 2 cut(s) 114, 806
BspPI GGATC 1 cut(s) 320
BspT104I TTCGAA 1 cut(s) 911
BspT107I GGYRCC 1 cut(s) 112
BsrI ACTGG 1 cut(s) 424
BssECI CCNNGG 1 cut(s) 436
BssMI GATC 2 cut(s) 198, 325
BssT1I CCWWGG 1 cut(s) 436
Bst6I CTCTTC 1 cut(s) 774
BstAPI GCANNNNNTGC 1 cut(s) 361
BstBI TTCGAA 1 cut(s) 911
BstDEI CTNAG 3 cut(s) 380, 591, 917
BstENI CCTNNNNNAGG 1 cut(s) 383
BstF5I GGATG 3 cut(s) 426, 620, 790
BstKTI GATC 2 cut(s) 201, 328
BstMAI GTCTC 1 cut(s) 163
BstMBI GATC 2 cut(s) 198, 325
BstMWI GCNNNNNNNGC 2 cut(s) 98, 361
BstNSI RCATGY 1 cut(s) 664
BstSLI GKGCMC 1 cut(s) 651
BstV1I GCAGC 3 cut(s) 31, 396, 716
BstXI CCANNNNNNTGG 1 cut(s) 437
Bsu36I CCTNAGG 1 cut(s) 380
BsuRI GGCC 2 cut(s) 423, 674
BtsCI GGATG 3 cut(s) 426, 620, 790
Csp6I GTAC 3 cut(s) 547, 848, 901
CviAII CATG 3 cut(s) 431, 642, 661
CviQI GTAC 3 cut(s) 547, 848, 901
DdeI CTNAG 3 cut(s) 380, 591, 917
DpnI GATC 2 cut(s) 200, 327
DpnII GATC 2 cut(s) 198, 325
EaeI YGGCCR 1 cut(s) 672
Eam1104I CTCTTC 1 cut(s) 774
EarI CTCTTC 1 cut(s) 774
Eco130I CCWWGG 1 cut(s) 436
Eco57I CTGAAG 2 cut(s) 393, 684
Eco81I CCTNAGG 1 cut(s) 380
Eco88I CYCGRG 1 cut(s) 23
EcoNI CCTNNNNNAGG 1 cut(s) 383
EcoT14I CCWWGG 1 cut(s) 436
ErhI CCWWGG 1 cut(s) 436
FaeI CATG 3 cut(s) 434, 645, 664
FalI AAGNNNNNCTT 2 cut(s) 435, 467
FatI CATG 3 cut(s) 430, 641, 660
FauI CCCGC 1 cut(s) 745
FblI GTMKAC 1 cut(s) 315
Fnu4HI GCNGC 3 cut(s) 20, 410, 705
FokI GGATG 3 cut(s) 413, 627, 797
Fsp4HI GCNGC 3 cut(s) 20, 410, 705
FspBI CTAG 1 cut(s) 459
GluI GCNGC 3 cut(s) 20, 410, 705
HaeIII GGCC 2 cut(s) 423, 674
Hin1II CATG 3 cut(s) 434, 645, 664
HincII GTYRAC 2 cut(s) 316, 856
HindII GTYRAC 2 cut(s) 316, 856
HinfI GANTC 1 cut(s) 621
HphI GGTGA 2 cut(s) 506, 880
Hpy166II GTNNAC 5 cut(s) 296, 316, 604, 620, 856
Hpy188I TCNGA 2 cut(s) 216, 610
Hpy188III TCNNGA 2 cut(s) 329, 563
Hpy8I GTNNAC 5 cut(s) 296, 316, 604, 620, 856
Hpy99I CGWCG 1 cut(s) 320
HpyAV CCTTC 5 cut(s) 155, 379, 708, 859, 918
HpyCH4IV ACGT 1 cut(s) 858
HpyCH4V TGCA 4 cut(s) 158, 232, 364, 412
HpyF10VI GCNNNNNNNGC 2 cut(s) 98, 361
HpyF3I CTNAG 3 cut(s) 380, 591, 917
HpySE526I ACGT 1 cut(s) 858
Hsp92II CATG 3 cut(s) 434, 645, 664
Kzo9I GATC 2 cut(s) 198, 325
LmnI GCTCC 3 cut(s) 469, 755, 810
LpnPI CCDG 7 cut(s) 39, 87, 209, 405, 519, 576, 809
Lsp1109I GCAGC 3 cut(s) 31, 396, 716
LweI GCATC 2 cut(s) 88, 145
MaeI CTAG 1 cut(s) 459
MaeII ACGT 1 cut(s) 858
MaeIII GTNAC 1 cut(s) 741
MalI GATC 2 cut(s) 200, 327
MboI GATC 2 cut(s) 198, 325
MboII GAAGA 4 cut(s) 413, 443, 446, 791
MhlI GDGCHC 2 cut(s) 357, 651
MlsI TGGCCA 1 cut(s) 674
MluCI AATT 5 cut(s) 45, 86, 124, 187, 257
MluNI TGGCCA 1 cut(s) 674
MlyI GAGTC 1 cut(s) 615
MnlI CCTC 6 cut(s) 301, 354, 434, 486, 604, 775
Mox20I TGGCCA 1 cut(s) 674
MscI TGGCCA 1 cut(s) 674
MseI TTAA 2 cut(s) 71, 770
Msp20I TGGCCA 1 cut(s) 674
MspA1I CMGCKG 1 cut(s) 371
Mva1269I GAATGC 1 cut(s) 293
MwoI GCNNNNNNNGC 2 cut(s) 98, 361
NdeII GATC 2 cut(s) 198, 325
NlaIII CATG 3 cut(s) 434, 645, 664
NlaIV GGNNCC 2 cut(s) 114, 806
NspI RCATGY 1 cut(s) 664
NspV TTCGAA 1 cut(s) 911
PciI ACATGT 1 cut(s) 660
PcsI WCGNNNNNNNCGW 2 cut(s) 312, 321
PctI GAATGC 1 cut(s) 293
PkrI GCNGC 3 cut(s) 21, 411, 706
PleI GAGTC 1 cut(s) 615
PpsI GAGTC 1 cut(s) 615
PscI ACATGT 1 cut(s) 660
PspN4I GGNNCC 2 cut(s) 114, 806
PvuII CAGCTG 1 cut(s) 371
RsaI GTAC 3 cut(s) 548, 849, 902
RsaNI GTAC 3 cut(s) 547, 848, 901
SalI GTCGAC 1 cut(s) 314
SaqAI TTAA 2 cut(s) 71, 770
SatI GCNGC 3 cut(s) 20, 410, 705
Sau3AI GATC 2 cut(s) 198, 325
ScaI AGTACT 2 cut(s) 548, 902
SchI GAGTC 1 cut(s) 615
SduI GDGCHC 2 cut(s) 357, 651
SfaNI GCATC 2 cut(s) 88, 145
SfuI TTCGAA 1 cut(s) 911
Sse9I AATT 5 cut(s) 45, 86, 124, 187, 257
SsiI CCGC 3 cut(s) 10, 554, 752
SspMI CTAG 1 cut(s) 459
StyI CCWWGG 1 cut(s) 436
TaiI ACGT 1 cut(s) 861
TaqI TCGA 5 cut(s) 315, 351, 624, 789, 911
TasI AATT 5 cut(s) 45, 86, 124, 187, 257
TatI WGTACW 2 cut(s) 546, 900
Tru1I TTAA 2 cut(s) 71, 770
Tru9I TTAA 2 cut(s) 71, 770
TseI GCWGC 3 cut(s) 19, 409, 704
TspDTI ATGAA 5 cut(s) 93, 117, 414, 527, 788
TspGWI ACGGA 1 cut(s) 797
XagI CCTNNNNNAGG 1 cut(s) 383
XapI RAATTY 1 cut(s) 187
XceI RCATGY 1 cut(s) 664
XmiI GTMKAC 1 cut(s) 315
XspI CTAG 1 cut(s) 459
ZrmI AGTACT 2 cut(s) 548, 902
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.