function. Its presence in a non-photosynthetic plant (Epifagus virginiana) and experiments in tobacco indicate that it has an essential function which is probably not related to photosynthesis
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | ATCG00860 ATCG01280 |
| fragaria_vesca | FvH4_c3g00160 |
| malus_domestica | MD15G1364000.v1.1 MD15G1364100.v1.1 MD15G1364300.v1.1 |
| pyrus_communis | pycom11g21060 pycom12397g00050 pycom12397g00150 pycom13g12670 pycom13g17800 pycom1682g00010 pycom3137g00010 pycom3137g00020 |
| rosa_chinensis | RchiOBHm_Chr2g0118461 RchiOBHm_Chr2g0118471 |
| rosa_laevigata | RLG00000000001 RLG00000000007 RLG00000000030 RLG00000000031 RLG00000000036 RLG00000000037 RLG00000000059 RLG00000000060 RLG00000000094 RLG00000000095 RLG00000000101 RLG00000000102 RLG00000000181 RLG00000000182 RLG00000000214 RLG00000000215 RLG00000000239 RLG00000000254 RLG00000000255 RLG00000000301 RLG00000000305 RLG00000000306 RLG00000016742 RLG00000029142 RLG00000037092 RLG00000037093 RLG00000037098 RLG00000037099 RLG00000037112 |
| rosa_rugosa | RorugMtG0003000.1 RorugMtG0003400.1 |
| rosa_samantha | Rh1BG209600 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 957 |
| AccB1I | GGYRCC | 1 cut(s) | 2763 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 1370, 1876 |
| AccBSI | CCGCTC | 1 cut(s) | 1886 |
| AciI | CCGC | 8 cut(s) | 157, 798, 825, 1390, 1884, 2708, 2848, 2990 |
| AcsI | RAATTY | 7 cut(s) | 268, 326, 378, 718, 1082, 1131, 3041 |
| AcuI | CTGAAG | 1 cut(s) | 368 |
| AcyI | GRCGYC | 1 cut(s) | 2764 |
| AfaI | GTAC | 7 cut(s) | 586, 2052, 2241, 2321, 2330, 2921, 3146 |
| AfiI | CCNNNNNNNGG | 9 cut(s) | 111, 830, 831, 1341, 1370, 1410, 1411, 1876, 2598 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 2416 |
| AjnI | CCWGG | 3 cut(s) | 1040, 1518, 1808 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 3078, 3110 |
| AloI | GAACNNNNNNTCC | 4 cut(s) | 564, 596, 1790, 1822 |
| AluBI | AGCT | 3 cut(s) | 1618, 1895, 3256 |
| AluI | AGCT | 3 cut(s) | 1618, 1895, 3256 |
| Alw26I | GTCTC | 4 cut(s) | 943, 1051, 1722, 2424 |
| Ama87I | CYCGRG | 2 cut(s) | 606, 753 |
| AoxI | GGCC | 4 cut(s) | 2079, 2494, 2588, 2798 |
| ApeKI | GCWGC | 1 cut(s) | 318 |
| ApoI | RAATTY | 7 cut(s) | 268, 326, 378, 718, 1082, 1131, 3041 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 1589, 1621, 1700, 1732 |
| Asp700I | GAANNNNTTC | 5 cut(s) | 215, 471, 722, 1514, 2006 |
| AspLEI | GCGC | 4 cut(s) | 1624, 1645, 2516, 2766 |
| AspS9I | GGNCC | 6 cut(s) | 821, 2028, 2080, 2589, 2799, 2983 |
| AsuC2I | CCSGG | 2 cut(s) | 1203, 3325 |
| AsuHPI | GGTGA | 3 cut(s) | 483, 1025, 1678 |
| AsuII | TTCGAA | 5 cut(s) | 636, 1698, 2334, 2962, 3150 |
| AvaI | CYCGRG | 2 cut(s) | 606, 753 |
| AvaII | GGWCC | 3 cut(s) | 821, 2028, 2983 |
| BamHI | GGATCC | 4 cut(s) | 1879, 2199, 2525, 2992 |
| BanI | GGYRCC | 1 cut(s) | 2763 |
| BanII | GRGCYC | 1 cut(s) | 2752 |
| BauI | CACGAG | 1 cut(s) | 2928 |
| BbsI | GAAGAC | 1 cut(s) | 392 |
| BbvI | GCAGC | 1 cut(s) | 330 |
| BccI | CCATC | 8 cut(s) | 109, 670, 926, 1372, 2864, 3168, 3235, 3308 |
| BciT130I | CCWGG | 3 cut(s) | 1042, 1520, 1810 |
| BciVI | GTATCC | 3 cut(s) | 248, 343, 711 |
| BclI | TGATCA | 3 cut(s) | 1126, 1156, 2644 |
| BcnI | CCSGG | 2 cut(s) | 1203, 3325 |
| BcoDI | GTCTC | 4 cut(s) | 943, 1051, 1722, 2424 |
| BfaI | CTAG | 8 cut(s) | 746, 776, 1410, 1469, 1833, 1980, 2486, 2754 |
| BfmI | CTRYAG | 2 cut(s) | 613, 1434 |
| BfoI | RGCGCY | 3 cut(s) | 1625, 2517, 2767 |
| BfuI | GTATCC | 3 cut(s) | 248, 343, 711 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 2772 |
| BglII | AGATCT | 3 cut(s) | 2056, 2135, 2936 |
| BisI | GCNGC | 2 cut(s) | 319, 799 |
| BlpI | GCTNAGC | 1 cut(s) | 1393 |
| BlsI | GCNGC | 2 cut(s) | 320, 800 |
| Bme1390I | CCNGG | 5 cut(s) | 1042, 1203, 1520, 1810, 3325 |
| Bme18I | GGWCC | 3 cut(s) | 821, 2028, 2983 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 2416 |
| BmeT110I | CYCGRG | 2 cut(s) | 606, 753 |
| BmgT120I | GGNCC | 6 cut(s) | 821, 2028, 2080, 2589, 2799, 2983 |
| BmrFI | CCNGG | 5 cut(s) | 1042, 1203, 1520, 1810, 3325 |
| BmsI | GCATC | 3 cut(s) | 43, 1650, 2607 |
| BpiI | GAAGAC | 1 cut(s) | 392 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1132, 1164 |
| BpmI | CTGGAG | 1 cut(s) | 1792 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1393 |
| Bpu14I | TTCGAA | 5 cut(s) | 636, 1698, 2334, 2962, 3150 |
| BpuEI | CTTGAG | 2 cut(s) | 167, 2391 |
| BpuMI | CCSGG | 2 cut(s) | 1203, 3325 |
| Bsa29I | ATCGAT | 3 cut(s) | 882, 891, 2087 |
| BsaBI | GATNNNNATC | 3 cut(s) | 699, 2481, 2565 |
| BsaHI | GRCGYC | 1 cut(s) | 2764 |
| BsaI | GGTCTC | 2 cut(s) | 1051, 2424 |
| BsaJI | CCNNGG | 8 cut(s) | 233, 1041, 1533, 1783, 1809, 2037, 2597, 2768 |
| BsaWI | WCCGGW | 1 cut(s) | 2518 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1790, 1820 |
| Bsc4I | CCNNNNNNNGG | 9 cut(s) | 111, 830, 831, 1341, 1370, 1410, 1411, 1876, 2598 |
| Bse1I | ACTGG | 1 cut(s) | 1447 |
| Bse3DI | GCAATG | 2 cut(s) | 1719, 2576 |
| Bse8I | GATNNNNATC | 3 cut(s) | 699, 2481, 2565 |
| BseBI | CCWGG | 3 cut(s) | 1042, 1520, 1810 |
| BseCI | ATCGAT | 3 cut(s) | 882, 891, 2087 |
| BseDI | CCNNGG | 8 cut(s) | 233, 1041, 1533, 1783, 1809, 2037, 2597, 2768 |
| BseGI | GGATG | 7 cut(s) | 101, 165, 1271, 1762, 2875, 3227, 3333 |
| BseJI | GATNNNNATC | 3 cut(s) | 699, 2481, 2565 |
| BseLI | CCNNNNNNNGG | 9 cut(s) | 111, 830, 831, 1341, 1370, 1410, 1411, 1876, 2598 |
| BseMI | GCAATG | 2 cut(s) | 1719, 2576 |
| BseMII | CTCAG | 2 cut(s) | 78, 503 |
| BseNI | ACTGG | 1 cut(s) | 1447 |
| BseRI | GAGGAG | 1 cut(s) | 2735 |
| BseXI | GCAGC | 1 cut(s) | 330 |
| Bsh1285I | CGRYCG | 2 cut(s) | 1600, 2403 |
| BshFI | GGCC | 4 cut(s) | 2081, 2496, 2590, 2800 |
| BshNI | GGYRCC | 1 cut(s) | 2763 |
| BshVI | ATCGAT | 3 cut(s) | 882, 891, 2087 |
| BsiEI | CGRYCG | 2 cut(s) | 1600, 2403 |
| BsiHKCI | CYCGRG | 2 cut(s) | 606, 753 |
| BsiSI | CCGG | 5 cut(s) | 1202, 1664, 2394, 2519, 3325 |
| BslFI | GGGAC | 2 cut(s) | 807, 2996 |
| BslI | CCNNNNNNNGG | 9 cut(s) | 111, 830, 831, 1341, 1370, 1410, 1411, 1876, 2598 |
| BsmAI | GTCTC | 4 cut(s) | 943, 1051, 1722, 2424 |
| BsmFI | GGGAC | 2 cut(s) | 807, 2996 |
| BsmI | GAATGC | 1 cut(s) | 1645 |
| BsnI | GGCC | 4 cut(s) | 2081, 2496, 2590, 2800 |
| Bso31I | GGTCTC | 2 cut(s) | 1051, 2424 |
| BsoBI | CYCGRG | 2 cut(s) | 606, 753 |
| Bsp119I | TTCGAA | 5 cut(s) | 636, 1698, 2334, 2962, 3150 |
| Bsp1286I | GDGCHC | 1 cut(s) | 2752 |
| Bsp1407I | TGTACA | 1 cut(s) | 2319 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1393 |
| Bsp19I | CCATGG | 2 cut(s) | 233, 2037 |
| BspACI | CCGC | 8 cut(s) | 157, 798, 825, 1390, 1884, 2708, 2848, 2990 |
| BspANI | GGCC | 4 cut(s) | 2081, 2496, 2590, 2800 |
| BspCNI | CTCAG | 2 cut(s) | 79, 504 |
| BspDI | ATCGAT | 3 cut(s) | 882, 891, 2087 |
| BspHI | TCATGA | 2 cut(s) | 343, 1747 |
| BspQI | GCTCTTC | 1 cut(s) | 2148 |
| BspT104I | TTCGAA | 5 cut(s) | 636, 1698, 2334, 2962, 3150 |
| BspT107I | GGYRCC | 1 cut(s) | 2763 |
| BspTNI | GGTCTC | 2 cut(s) | 1051, 2424 |
| BsrBI | CCGCTC | 1 cut(s) | 1886 |
| BsrDI | GCAATG | 2 cut(s) | 1719, 2576 |
| BsrGI | TGTACA | 1 cut(s) | 2319 |
| BsrI | ACTGG | 1 cut(s) | 1447 |
| BssECI | CCNNGG | 8 cut(s) | 233, 1041, 1533, 1783, 1809, 2037, 2597, 2768 |
| BssNI | GRCGYC | 1 cut(s) | 2764 |
| BssSI | CACGAG | 1 cut(s) | 2928 |
| BssT1I | CCWWGG | 5 cut(s) | 233, 1533, 2037, 2597, 2768 |
| Bst2BI | CACGAG | 1 cut(s) | 2928 |
| Bst2UI | CCWGG | 3 cut(s) | 1042, 1520, 1810 |
| Bst4CI | ACNGT | 4 cut(s) | 1013, 1055, 1501, 1601 |
| Bst6I | CTCTTC | 3 cut(s) | 2148, 2859, 3051 |
| BstACI | GRCGYC | 1 cut(s) | 2764 |
| BstAUI | TGTACA | 1 cut(s) | 2319 |
| BstBI | TTCGAA | 5 cut(s) | 636, 1698, 2334, 2962, 3150 |
| BstC8I | GCNNGC | 2 cut(s) | 1620, 2846 |
| BstDSI | CCRYGG | 2 cut(s) | 233, 2037 |
| BstF5I | GGATG | 7 cut(s) | 101, 165, 1271, 1762, 2875, 3227, 3333 |
| BstH2I | RGCGCY | 3 cut(s) | 1625, 2517, 2767 |
| BstHHI | GCGC | 4 cut(s) | 1624, 1645, 2516, 2766 |
| BstMAI | GTCTC | 4 cut(s) | 943, 1051, 1722, 2424 |
| BstMCI | CGRYCG | 2 cut(s) | 1600, 2403 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 1892, 2513, 2587, 2772 |
| BstNI | CCWGG | 3 cut(s) | 1042, 1520, 1810 |
| BstNSI | RCATGY | 2 cut(s) | 808, 1917 |
| BstSCI | CCNGG | 5 cut(s) | 1040, 1201, 1518, 1808, 3323 |
| BstSFI | CTRYAG | 2 cut(s) | 613, 1434 |
| BstV1I | GCAGC | 1 cut(s) | 330 |
| BstV2I | GAAGAC | 1 cut(s) | 392 |
| BstXI | CCANNNNNNTGG | 3 cut(s) | 2018, 2038, 2809 |
| Bsu15I | ATCGAT | 3 cut(s) | 882, 891, 2087 |
| BsuI | GTATCC | 3 cut(s) | 248, 343, 711 |
| BsuRI | GGCC | 4 cut(s) | 2081, 2496, 2590, 2800 |
| BsuTUI | ATCGAT | 3 cut(s) | 882, 891, 2087 |
| BtgI | CCRYGG | 2 cut(s) | 233, 2037 |
| BtgZI | GCGATG | 1 cut(s) | 3149 |
| BtsCI | GGATG | 7 cut(s) | 101, 165, 1271, 1762, 2875, 3227, 3333 |
| BtsIMutI | CAGTG | 1 cut(s) | 1060 |
| Cac8I | GCNNGC | 2 cut(s) | 1620, 2846 |
| CciI | TCATGA | 2 cut(s) | 343, 1747 |
| CfoI | GCGC | 4 cut(s) | 1624, 1645, 2516, 2766 |
| Cfr13I | GGNCC | 6 cut(s) | 821, 2028, 2080, 2589, 2799, 2983 |
| ClaI | ATCGAT | 3 cut(s) | 882, 891, 2087 |
| Csp6I | GTAC | 7 cut(s) | 585, 2051, 2240, 2320, 2329, 2920, 3145 |
| CviQI | GTAC | 7 cut(s) | 585, 2051, 2240, 2320, 2329, 2920, 3145 |
| DinI | GGCGCC | 1 cut(s) | 2765 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 957 |
| DriI | GACNNNNNGTC | 1 cut(s) | 2416 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 957 |
| Eam1104I | CTCTTC | 3 cut(s) | 2148, 2859, 3051 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 2416 |
| EarI | CTCTTC | 3 cut(s) | 2148, 2859, 3051 |
| EciI | GGCGGA | 1 cut(s) | 172 |
| Eco130I | CCWWGG | 5 cut(s) | 233, 1533, 2037, 2597, 2768 |
| Eco24I | GRGCYC | 1 cut(s) | 2752 |
| Eco31I | GGTCTC | 2 cut(s) | 1051, 2424 |
| Eco32I | GATATC | 3 cut(s) | 501, 575, 886 |
| Eco47I | GGWCC | 3 cut(s) | 821, 2028, 2983 |
| Eco57I | CTGAAG | 1 cut(s) | 368 |
| Eco88I | CYCGRG | 2 cut(s) | 606, 753 |
| EcoO109I | RGGNCCY | 2 cut(s) | 2080, 2983 |
| EcoRI | GAATTC | 4 cut(s) | 378, 718, 1082, 3041 |
| EcoRII | CCWGG | 3 cut(s) | 1040, 1518, 1808 |
| EcoRV | GATATC | 3 cut(s) | 501, 575, 886 |
| EcoT14I | CCWWGG | 5 cut(s) | 233, 1533, 2037, 2597, 2768 |
| EcoT22I | ATGCAT | 2 cut(s) | 357, 1919 |
| EcoT38I | GRGCYC | 1 cut(s) | 2752 |
| EgeI | GGCGCC | 1 cut(s) | 2765 |
| EheI | GGCGCC | 1 cut(s) | 2765 |
| ErhI | CCWWGG | 5 cut(s) | 233, 1533, 2037, 2597, 2768 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 725, 757 |
| FaqI | GGGAC | 2 cut(s) | 807, 2996 |
| FauI | CCCGC | 3 cut(s) | 832, 1891, 2715 |
| FbaI | TGATCA | 3 cut(s) | 1126, 1156, 2644 |
| Fnu4HI | GCNGC | 2 cut(s) | 319, 799 |
| FokI | GGATG | 6 cut(s) | 88, 172, 1258, 1769, 2882, 3214 |
| FriOI | GRGCYC | 1 cut(s) | 2752 |
| Fsp4HI | GCNGC | 2 cut(s) | 319, 799 |
| FspBI | CTAG | 8 cut(s) | 746, 776, 1410, 1469, 1833, 1980, 2486, 2754 |
| GlaI | GCGC | 4 cut(s) | 1623, 1644, 2515, 2765 |
| GluI | GCNGC | 2 cut(s) | 319, 799 |
| GsuI | CTGGAG | 1 cut(s) | 1792 |
| HaeII | RGCGCY | 3 cut(s) | 1625, 2517, 2767 |
| HaeIII | GGCC | 4 cut(s) | 2081, 2496, 2590, 2800 |
| HapII | CCGG | 5 cut(s) | 1202, 1664, 2394, 2519, 3325 |
| HhaI | GCGC | 4 cut(s) | 1624, 1645, 2516, 2766 |
| Hin1I | GRCGYC | 1 cut(s) | 2764 |
| Hin6I | GCGC | 4 cut(s) | 1622, 1643, 2514, 2764 |
| HinP1I | GCGC | 4 cut(s) | 1622, 1643, 2514, 2764 |
| HincII | GTYRAC | 1 cut(s) | 1231 |
| HindII | GTYRAC | 1 cut(s) | 1231 |
| HpaII | CCGG | 5 cut(s) | 1202, 1664, 2394, 2519, 3325 |
| HphI | GGTGA | 3 cut(s) | 483, 1025, 1678 |
| Hpy166II | GTNNAC | 5 cut(s) | 443, 587, 1009, 1231, 1798 |
| Hpy8I | GTNNAC | 5 cut(s) | 443, 587, 1009, 1231, 1798 |
| Hpy99I | CGWCG | 1 cut(s) | 1580 |
| HpyAV | CCTTC | 9 cut(s) | 14, 566, 1700, 1923, 2214, 2297, 2504, 2681, 2687 |
| HpyCH4III | ACNGT | 4 cut(s) | 1013, 1055, 1501, 1601 |
| HpyCH4IV | ACGT | 2 cut(s) | 847, 1005 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 1892, 2513, 2587, 2772 |
| HpySE526I | ACGT | 2 cut(s) | 847, 1005 |
| Hsp92I | GRCGYC | 1 cut(s) | 2764 |
| HspAI | GCGC | 4 cut(s) | 1622, 1643, 2514, 2764 |
| KasI | GGCGCC | 1 cut(s) | 2763 |
| KflI | GGGWCCC | 1 cut(s) | 2983 |
| Ksp22I | TGATCA | 3 cut(s) | 1126, 1156, 2644 |
| LguI | GCTCTTC | 1 cut(s) | 2148 |
| LmnI | GCTCC | 7 cut(s) | 1205, 1392, 1615, 1900, 2261, 2521, 2755 |
| Lsp1109I | GCAGC | 1 cut(s) | 330 |
| LweI | GCATC | 3 cut(s) | 43, 1650, 2607 |
| MaeI | CTAG | 8 cut(s) | 746, 776, 1410, 1469, 1833, 1980, 2486, 2754 |
| MaeII | ACGT | 2 cut(s) | 847, 1005 |
| MaeIII | GTNAC | 7 cut(s) | 489, 621, 1556, 1592, 1601, 2297, 2572 |
| MbiI | CCGCTC | 1 cut(s) | 1886 |
| MhlI | GDGCHC | 1 cut(s) | 2752 |
| Mly113I | GGCGCC | 1 cut(s) | 2764 |
| MlyI | GAGTC | 7 cut(s) | 497, 1141, 1150, 1775, 1852, 2457, 2706 |
| Mph1103I | ATGCAT | 2 cut(s) | 357, 1919 |
| MroXI | GAANNNNTTC | 5 cut(s) | 215, 471, 722, 1514, 2006 |
| MseI | TTAA | 6 cut(s) | 731, 1106, 1907, 1920, 2360, 3158 |
| MslI | CAYNNNNRTG | 2 cut(s) | 2016, 2036 |
| MspI | CCGG | 5 cut(s) | 1202, 1664, 2394, 2519, 3325 |
| MspR9I | CCNGG | 5 cut(s) | 1042, 1203, 1520, 1810, 3325 |
| Mva1269I | GAATGC | 1 cut(s) | 1645 |
| MvaI | CCWGG | 3 cut(s) | 1042, 1520, 1810 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 1892, 2513, 2587, 2772 |
| NarI | GGCGCC | 1 cut(s) | 2764 |
| NciI | CCSGG | 2 cut(s) | 1203, 3325 |
| NcoI | CCATGG | 2 cut(s) | 233, 2037 |
| NmuCI | GTSAC | 5 cut(s) | 489, 1556, 1592, 1601, 2572 |
| NsiI | ATGCAT | 2 cut(s) | 357, 1919 |
| NspI | RCATGY | 2 cut(s) | 808, 1917 |
| NspV | TTCGAA | 5 cut(s) | 636, 1698, 2334, 2962, 3150 |
| PaeR7I | CTCGAG | 1 cut(s) | 606 |
| PagI | TCATGA | 2 cut(s) | 343, 1747 |
| PciSI | GCTCTTC | 1 cut(s) | 2148 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 888, 944 |
| PctI | GAATGC | 1 cut(s) | 1645 |
| PdmI | GAANNNNTTC | 5 cut(s) | 215, 471, 722, 1514, 2006 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 1370, 1876 |
| PfoI | TCCNGGA | 1 cut(s) | 3323 |
| PkrI | GCNGC | 2 cut(s) | 320, 800 |
| PleI | GAGTC | 7 cut(s) | 496, 1141, 1149, 1774, 1852, 2457, 2705 |
| PluTI | GGCGCC | 1 cut(s) | 2767 |
| PpsI | GAGTC | 7 cut(s) | 496, 1141, 1149, 1774, 1852, 2457, 2705 |
| PpuMI | RGGWCCY | 1 cut(s) | 2983 |
| Psp5II | RGGWCCY | 1 cut(s) | 2983 |
| Psp6I | CCWGG | 3 cut(s) | 1040, 1518, 1808 |
| PspGI | CCWGG | 3 cut(s) | 1040, 1518, 1808 |
| PspPI | GGNCC | 6 cut(s) | 821, 2028, 2080, 2589, 2799, 2983 |
| PspPPI | RGGWCCY | 1 cut(s) | 2983 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 2169, 2201 |
| RsaI | GTAC | 7 cut(s) | 586, 2052, 2241, 2321, 2330, 2921, 3146 |
| RsaNI | GTAC | 7 cut(s) | 585, 2051, 2240, 2320, 2329, 2920, 3145 |
| RseI | CAYNNNNRTG | 2 cut(s) | 2016, 2036 |
| SapI | GCTCTTC | 1 cut(s) | 2148 |
| SaqAI | TTAA | 6 cut(s) | 731, 1106, 1907, 1920, 2360, 3158 |
| SatI | GCNGC | 2 cut(s) | 319, 799 |
| Sau96I | GGNCC | 6 cut(s) | 821, 2028, 2080, 2589, 2799, 2983 |
| SchI | GAGTC | 7 cut(s) | 497, 1141, 1150, 1775, 1852, 2457, 2706 |
| ScrFI | CCNGG | 5 cut(s) | 1042, 1203, 1520, 1810, 3325 |
| SduI | GDGCHC | 1 cut(s) | 2752 |
| SfaNI | GCATC | 3 cut(s) | 43, 1650, 2607 |
| SfcI | CTRYAG | 2 cut(s) | 613, 1434 |
| SfoI | GGCGCC | 1 cut(s) | 2765 |
| Sfr274I | CTCGAG | 1 cut(s) | 606 |
| SfuI | TTCGAA | 5 cut(s) | 636, 1698, 2334, 2962, 3150 |
| SinI | GGWCC | 3 cut(s) | 821, 2028, 2983 |
| SlaI | CTCGAG | 1 cut(s) | 606 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 2016, 2036 |
| SmlI | CTYRAG | 3 cut(s) | 146, 606, 2406 |
| SmoI | CTYRAG | 3 cut(s) | 146, 606, 2406 |
| SsiI | CCGC | 8 cut(s) | 157, 798, 825, 1390, 1884, 2708, 2848, 2990 |
| SspDI | GGCGCC | 1 cut(s) | 2763 |
| SspI | AATATT | 3 cut(s) | 95, 863, 1840 |
| SspMI | CTAG | 8 cut(s) | 746, 776, 1410, 1469, 1833, 1980, 2486, 2754 |
| StyD4I | CCNGG | 5 cut(s) | 1040, 1201, 1518, 1808, 3323 |
| StyI | CCWWGG | 5 cut(s) | 233, 1533, 2037, 2597, 2768 |
| TaaI | ACNGT | 4 cut(s) | 1013, 1055, 1501, 1601 |
| TaiI | ACGT | 2 cut(s) | 850, 1008 |
| TaqII | GACCGA | 3 cut(s) | 1689, 1775, 2389 |
| TatI | WGTACW | 2 cut(s) | 2239, 2319 |
| TauI | GCSGC | 1 cut(s) | 801 |
| Tru1I | TTAA | 6 cut(s) | 731, 1106, 1907, 1920, 2360, 3158 |
| Tru9I | TTAA | 6 cut(s) | 731, 1106, 1907, 1920, 2360, 3158 |
| TscAI | CASTG | 1 cut(s) | 1060 |
| TseFI | GTSAC | 5 cut(s) | 489, 1556, 1592, 1601, 2572 |
| TseI | GCWGC | 1 cut(s) | 318 |
| Tsp45I | GTSAC | 5 cut(s) | 489, 1556, 1592, 1601, 2572 |
| TspGWI | ACGGA | 6 cut(s) | 1306, 1628, 2548, 2728, 3104, 3285 |
| TspRI | CASTG | 1 cut(s) | 1060 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 1370, 1876 |
| VpaK11BI | GGWCC | 3 cut(s) | 821, 2028, 2983 |
| XapI | RAATTY | 7 cut(s) | 268, 326, 378, 718, 1082, 1131, 3041 |
| XbaI | TCTAGA | 1 cut(s) | 775 |
| XceI | RCATGY | 2 cut(s) | 808, 1917 |
| XhoI | CTCGAG | 1 cut(s) | 606 |
| XmnI | GAANNNNTTC | 5 cut(s) | 215, 471, 722, 1514, 2006 |
| XspI | CTAG | 8 cut(s) | 746, 776, 1410, 1469, 1833, 1980, 2486, 2754 |
| Zsp2I | ATGCAT | 2 cut(s) | 357, 1919 |