pycom3137g00020

Plant protein of unknown function (DUF825)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00003137
OG0001704
Physical Location & Seq
Reverse (-)
7657 .. 10252
2596 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2451 bp
ATGAAAGGACATCAATTCAAATCCTGGATTTTCGAATTGAGAGAGATATTGAGAGAGATCCAGAATTCTCACTATTTCTTAGATTCATGGACCAAATTCAATTCAGTGGGATCTTTTATTCACATTTTTTTCCATCAAGAACGTTTTATAAAACTCTTGGACTCCCGAATTTGGAGTATCCTACTTTCACGCAATTCACATTTACAGGGTTCAACAAGCAATCGATATTTCACGATCAAGGGTGTAGTACTATTTGTAGTAGCGGTCCTTATATATCGTATTAACAATCGAAAGATGGTCGAAAGAAAAAATCTCTATTTGACAGGGCTTCTTCCTATACCTATGAATTTCATTGGACCCAGAAATGATACATTGGAAGAATCTTTTGGGTCTTCCAATATCAATAGGTTGATTGTTTCGCTCCTGTATCTTCCAAAAGGAAAAAAGATCTCTGAGAGCTGTTTCCTGGATCCGAAAGAGAGTACTTGGGTTCTCCCAATAACTAAAAAGTGTATCATGCCTGAATCTAACTGGGGTTCGCGGTGGTGGAGGAACTGGATCGGAAAAAAGAGGGCTTCTAGTTTTTCTTTTGTATATTATATGGATGATCCGATCCGCAAGGACCATGATTGGGAATTGTTTGATCGTCTTTCTCCGAGGAAGAGGCGAAACATAATCAACTTGAATTCGGGACAGCTATTCGAAATCTTAGTGAAAGACTGGATTTGTTATCTCATGTTTGCTTTTCGTGAAAAAATACCAATTGAAGCGGAGGGTTTCTTCAAACAACAAGGAGCTGGGTCAACTATTCAATCAAATGAAATTGAGCATGTTTCCCATCTCTTCTCGAGAAACAAGTGGGCTATGCAAAATTGTGCTCAATTTCATATGCGGCAATTCCGCCAAGATCTCTTCTTTAGTTGGGGGAAGAATCCGCACGAATCGGATTTTTTGAGGAACATATCGAGAGAGAATTGGTTAGACAATGTGTGGTTGTTAAACAAGGATCGGTTTTTTAGCAAGGTACGGAATGCATCGTCAAATATTCAATATGATTCCACAAGATCTAGTTTCGTTCAAGTAACGGATTCTAGCCAATTGAAAGGATCCTCTGATCAATCCAGAGATCATTTCGATTCCATTAGTAATGAGGATTCAGAATATCACACATTGATCAATCAAAGAGAGATTCAACAACTAAAAGAAAGATCGATTCTTTGGGATCCTTCTTTTCTTCAAACGGAACGAACAGAGATAGAATCAGACCGATTCCCTAAATGCCTTTCTGGATATTCCTCAATGTCCCGGCTATTCACGGAACGTGAGAAGCAGATGAATAAGCATCTGCTTCCGGAAGAAATCGAAGAATTTCTTGGGAATCCTACAAGATCCATTCGTTCTTTTTTCTCTGACAGATGGTCAGAACTTCATCTGGGTTCGAATCCTACTGAGAGGTCCACTAGAGATCAGAAATTGTTGAAGAAAGAACAAGATGTTTCTTTTGTCCCTTCCAGGCGATCGGAAAATAAAGAAATAGTTAATATATTCAAGATAATTACGTATTTACAAAATACCGTCTCAATTCATCCTATTTCATCAGATCCGGGATGTGATATGGTTCCGAAGGATGAATTGGATATGGACAGTTCCAATAAGATTTCATTCTTGAACAAAAATCCATTTTTTGATTTATTTCATCTATTCCATGACCGGAACAGGGGGGGATACACGTTACACCACGATTTTGAATCAGAAGAGAGATTTCAAGAAATGGCAGATCTATTCACTCTATCAATAACCGAGCCGGATCTGGTGTATCATAAGGGATTTGCCTTTTCTATTGGATTGGATCAAAAACAATTCTTGAATGAGGTATTCAACTCCAGGGATGAATCGAAAAAGAAATCTTTATTGGTTCTACCTCCTATTTTTTATGAAGAGAATGAATCTTTTTATCGAAGGATCAGAAAAAAATGGGTCCGGACCTCCTGCGGGAATGATTTGGAAGATCCAAAATCAAAAATAGTGGTATTTGCTAGCAACAACATAATGGAGGCAGTCAATCAATATAGATTGATCCGAAATCTGATTCAAATCCAATATAGCACCTATGGGTACATAAGAAATGTATTGAATCGATTCTTTTTAATGAATAGATCCGATCGCAACTTCGAATATGGAATTCAAAGGGATCAAATAGGAAATGATACTCTGAATCATAGAACTATAATGAAATATACGATCAACCAACATTTATCAAATTTGAAAAAGAGTCAGAAGAAATGGTTCGATCCTCTTATTTTTATTTCTCGAACCGAGAGATCCATGAATCGGGATCCTAATGCATATAGATACAAATGGTCCAATGGGAGCAAGAATTTACAGGAACATTTGGAACATTTCATTTCTGAGCAGAAGAGCCGTTTTCATTTTCAAGTAGTATTTGTCTAA

Protein Analysis

817

Amino Acids

97.33

Weight (kDa)

9.27

Isoelectric Point (pI)

51.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ycf2 PF05695 1 - 815 0.0 Ycf2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 149
AccII CGCG 1 cut(s) 541
AccIII TCCGGA 2 cut(s) 1351, 1980
AciI CCGC 8 cut(s) 263, 541, 616, 770, 892, 901, 935, 1992
AclI AACGTT 1 cut(s) 142
AcsI RAATTY 9 cut(s) 64, 95, 168, 346, 685, 1367, 2181, 2260, 2377
AfaI GTAC 4 cut(s) 249, 484, 1026, 2117
AfiI CCNNNNNNNGG 5 cut(s) 171, 631, 1717, 1992, 2331
AflIII ACRYGT 1 cut(s) 1728
AhdI GACNNNNNGTC 1 cut(s) 1417
AjnI CCWGG 4 cut(s) 23, 465, 1511, 1883
AjuI GAANNNNNNNTTGG 8 cut(s) 369, 401, 1356, 1388, 1616, 1648, 1895, 1927
AluBI AGCT 3 cut(s) 459, 697, 797
AluI AGCT 3 cut(s) 459, 697, 797
Alw21I GWGCWC 1 cut(s) 880
Alw26I GTCTC 1 cut(s) 1582
Ama87I CYCGRG 1 cut(s) 847
Aor13HI TCCGGA 2 cut(s) 1351, 1980
ApoI RAATTY 9 cut(s) 64, 95, 168, 346, 685, 1367, 2181, 2260, 2377
Asp700I GAANNNNTTC 3 cut(s) 1368, 2138, 2285
AspS9I GGNCC 8 cut(s) 90, 265, 356, 622, 1455, 1978, 1983, 2361
AsuC2I CCSGG 2 cut(s) 1306, 1605
AsuII TTCGAA 4 cut(s) 33, 702, 1439, 2172
AsuNHI GCTAGC 1 cut(s) 2036
AvaI CYCGRG 1 cut(s) 847
AvaII GGWCC 8 cut(s) 90, 265, 356, 622, 1455, 1978, 1983, 2361
BamHI GGATCC 4 cut(s) 469, 1106, 1222, 2335
BbsI GAAGAC 1 cut(s) 384
Bbv12I GWGCWC 1 cut(s) 880
BccI CCATC 4 cut(s) 141, 289, 846, 1410
BceAI ACGGC 1 cut(s) 2406
BciT130I CCWGG 4 cut(s) 25, 467, 1513, 1885
BciVI GTATCC 2 cut(s) 188, 1718
BclI TGATCA 2 cut(s) 1114, 1173
BcnI CCSGG 2 cut(s) 1306, 1605
BcoDI GTCTC 1 cut(s) 1582
BfaI CTAG 5 cut(s) 579, 1068, 1092, 1461, 2037
BfuI GTATCC 2 cut(s) 188, 1718
BglII AGATCT 4 cut(s) 447, 907, 1064, 1777
BisI GCNGC 1 cut(s) 893
BlsI GCNGC 1 cut(s) 894
BmcAI AGTACT 2 cut(s) 249, 484
Bme1390I CCNGG 6 cut(s) 25, 467, 1306, 1513, 1605, 1885
Bme18I GGWCC 8 cut(s) 90, 265, 356, 622, 1455, 1978, 1983, 2361
BmeRI GACNNNNNGTC 1 cut(s) 1417
BmeT110I CYCGRG 1 cut(s) 847
BmgT120I GGNCC 8 cut(s) 90, 265, 356, 622, 1455, 1978, 1983, 2361
BmiI GGNNCC 7 cut(s) 358, 471, 1108, 1224, 1620, 1979, 2337
BmrFI CCNGG 6 cut(s) 25, 467, 1306, 1513, 1605, 1885
BmrI ACTGGG 1 cut(s) 541
BmsI GCATC 2 cut(s) 1043, 1351
BmtI GCTAGC 1 cut(s) 2040
BmuI ACTGGG 1 cut(s) 541
BpiI GAAGAC 1 cut(s) 384
BpmI CTGGAG 1 cut(s) 1867
Bpu14I TTCGAA 4 cut(s) 33, 702, 1439, 2172
BpuMI CCSGG 2 cut(s) 1306, 1605
Bsa29I ATCGAT 3 cut(s) 223, 1211, 2137
BsaAI YACGTR 1 cut(s) 1560
BsaBI GATNNNNATC 1 cut(s) 2093
BsaJI CCNNGG 2 cut(s) 656, 1884
BsaWI WCCGGW 3 cut(s) 1351, 1710, 1980
Bsc4I CCNNNNNNNGG 5 cut(s) 171, 631, 1717, 1992, 2331
Bse1I ACTGG 3 cut(s) 536, 560, 725
Bse8I GATNNNNATC 1 cut(s) 2093
BseAI TCCGGA 2 cut(s) 1351, 1980
BseBI CCWGG 4 cut(s) 25, 467, 1513, 1885
BseCI ATCGAT 3 cut(s) 223, 1211, 2137
BseDI CCNNGG 2 cut(s) 656, 1884
BseGI GGATG 5 cut(s) 610, 1585, 1613, 1633, 1894
BseJI GATNNNNATC 1 cut(s) 2093
BseLI CCNNNNNNNGG 5 cut(s) 171, 631, 1717, 1992, 2331
BseMII CTCAG 3 cut(s) 444, 1440, 2400
BseNI ACTGG 3 cut(s) 536, 560, 725
BseYI CCCAGC 1 cut(s) 797
Bsh1236I CGCG 1 cut(s) 541
Bsh1285I CGRYCG 2 cut(s) 1520, 2164
BshVI ATCGAT 3 cut(s) 223, 1211, 2137
BsiEI CGRYCG 2 cut(s) 1520, 2164
BsiHKAI GWGCWC 1 cut(s) 880
BsiHKCI CYCGRG 1 cut(s) 847
BsiSI CCGG 6 cut(s) 1306, 1352, 1604, 1711, 1805, 1981
BslFI GGGAC 3 cut(s) 705, 1288, 1490
BslI CCNNNNNNNGG 5 cut(s) 171, 631, 1717, 1992, 2331
BsmAI GTCTC 1 cut(s) 1582
BsmBI CGTCTC 1 cut(s) 1582
BsmFI GGGAC 3 cut(s) 705, 1288, 1490
BsmI GAATGC 1 cut(s) 1036
BsoBI CYCGRG 1 cut(s) 847
Bsp119I TTCGAA 4 cut(s) 33, 702, 1439, 2172
Bsp1286I GDGCHC 1 cut(s) 880
Bsp13I TCCGGA 2 cut(s) 1351, 1980
BspACI CCGC 8 cut(s) 263, 541, 616, 770, 892, 901, 935, 1992
BspCNI CTCAG 3 cut(s) 445, 1441, 2401
BspDI ATCGAT 3 cut(s) 223, 1211, 2137
BspEI TCCGGA 2 cut(s) 1351, 1980
BspFNI CGCG 1 cut(s) 541
BspLI GGNNCC 7 cut(s) 358, 471, 1108, 1224, 1620, 1979, 2337
BspOI GCTAGC 1 cut(s) 2040
BspQI GCTCTTC 1 cut(s) 2411
BspT104I TTCGAA 4 cut(s) 33, 702, 1439, 2172
BsrI ACTGG 3 cut(s) 536, 560, 725
BssECI CCNNGG 2 cut(s) 656, 1884
Bst2UI CCWGG 4 cut(s) 25, 467, 1513, 1885
Bst4CI ACNGT 2 cut(s) 1576, 1646
Bst6I CTCTTC 6 cut(s) 656, 848, 917, 1749, 1932, 2411
BstBAI YACGTR 1 cut(s) 1560
BstBI TTCGAA 4 cut(s) 33, 702, 1439, 2172
BstC8I GCNNGC 1 cut(s) 2038
BstDEI CTNAG 5 cut(s) 79, 453, 709, 1449, 2409
BstF5I GGATG 5 cut(s) 610, 1585, 1613, 1633, 1894
BstFNI CGCG 1 cut(s) 541
BstMAI GTCTC 1 cut(s) 1582
BstMCI CGRYCG 2 cut(s) 1520, 2164
BstNI CCWGG 4 cut(s) 25, 467, 1513, 1885
BstNSI RCATGY 1 cut(s) 833
BstSCI CCNGG 6 cut(s) 23, 465, 1304, 1511, 1603, 1883
BstSNI TACGTA 1 cut(s) 1560
BstUI CGCG 1 cut(s) 541
BstV2I GAAGAC 1 cut(s) 384
Bsu15I ATCGAT 3 cut(s) 223, 1211, 2137
BsuI GTATCC 2 cut(s) 188, 1718
BsuTUI ATCGAT 3 cut(s) 223, 1211, 2137
BtsCI GGATG 5 cut(s) 610, 1585, 1613, 1633, 1894
BtsIMutI CAGTG 1 cut(s) 111
Cac8I GCNNGC 1 cut(s) 2038
Cfr13I GGNCC 8 cut(s) 90, 265, 356, 622, 1455, 1978, 1983, 2361
ClaI ATCGAT 3 cut(s) 223, 1211, 2137
Csp6I GTAC 4 cut(s) 248, 483, 1025, 2116
CspCI CAANNNNNGTGG 2 cut(s) 2007, 2042
CviAII CATG 7 cut(s) 87, 517, 626, 736, 830, 1706, 2326
CviQI GTAC 4 cut(s) 248, 483, 1025, 2116
DdeI CTNAG 5 cut(s) 79, 453, 709, 1449, 2409
DriI GACNNNNNGTC 1 cut(s) 1417
Eam1104I CTCTTC 6 cut(s) 656, 848, 917, 1749, 1932, 2411
Eam1105I GACNNNNNGTC 1 cut(s) 1417
EarI CTCTTC 6 cut(s) 656, 848, 917, 1749, 1932, 2411
EciI GGCGGA 1 cut(s) 890
Eco105I TACGTA 1 cut(s) 1560
Eco47I GGWCC 8 cut(s) 90, 265, 356, 622, 1455, 1978, 1983, 2361
Eco88I CYCGRG 1 cut(s) 847
EcoRI GAATTC 3 cut(s) 64, 685, 2181
EcoRII CCWGG 4 cut(s) 23, 465, 1511, 1883
EcoT22I ATGCAT 2 cut(s) 1036, 2347
Esp3I CGTCTC 1 cut(s) 1582
FaeI CATG 7 cut(s) 90, 520, 629, 739, 833, 1709, 2329
FalI AAGNNNNNCTT 2 cut(s) 1356, 1388
FaqI GGGAC 3 cut(s) 705, 1288, 1490
FatI CATG 7 cut(s) 86, 516, 625, 735, 829, 1705, 2325
FauI CCCGC 1 cut(s) 1985
FauNDI CATATG 1 cut(s) 888
FbaI TGATCA 2 cut(s) 1114, 1173
Fnu4HI GCNGC 1 cut(s) 893
FokI GGATG 5 cut(s) 617, 1572, 1620, 1640, 1901
Fsp4HI GCNGC 1 cut(s) 893
FspBI CTAG 5 cut(s) 579, 1068, 1092, 1461, 2037
GluI GCNGC 1 cut(s) 893
GsaI CCCAGC 1 cut(s) 801
GsuI CTGGAG 1 cut(s) 1867
HapII CCGG 6 cut(s) 1306, 1352, 1604, 1711, 1805, 1981
Hin1II CATG 7 cut(s) 90, 520, 629, 739, 833, 1709, 2329
HincII GTYRAC 1 cut(s) 804
HindII GTYRAC 1 cut(s) 804
HpaII CCGG 6 cut(s) 1306, 1352, 1604, 1711, 1805, 1981
Hpy166II GTNNAC 2 cut(s) 804, 1458
Hpy8I GTNNAC 2 cut(s) 804, 1458
HpyAV CCTTC 4 cut(s) 1236, 1518, 1618, 1953
HpyCH4III ACNGT 2 cut(s) 1576, 1646
HpyCH4IV ACGT 4 cut(s) 142, 1321, 1559, 1730
HpyCH4V TGCA 3 cut(s) 868, 1034, 2345
HpyF3I CTNAG 5 cut(s) 79, 453, 709, 1449, 2409
HpySE526I ACGT 4 cut(s) 142, 1321, 1559, 1730
Hsp92II CATG 7 cut(s) 90, 520, 629, 739, 833, 1709, 2329
Kpn2I TCCGGA 2 cut(s) 1351, 1980
Ksp22I TGATCA 2 cut(s) 1114, 1173
LguI GCTCTTC 1 cut(s) 2411
LmnI GCTCC 3 cut(s) 426, 794, 2370
LweI GCATC 2 cut(s) 1043, 1351
MaeI CTAG 5 cut(s) 579, 1068, 1092, 1461, 2037
MaeII ACGT 4 cut(s) 142, 1321, 1559, 1730
MaeIII GTNAC 2 cut(s) 1081, 1731
MfeI CAATTG 2 cut(s) 762, 1097
MhlI GDGCHC 1 cut(s) 880
MlyI GAGTC 2 cut(s) 155, 2281
Mph1103I ATGCAT 2 cut(s) 1036, 2347
MroI TCCGGA 2 cut(s) 1351, 1980
MroXI GAANNNNTTC 3 cut(s) 1368, 2138, 2285
MseI TTAA 4 cut(s) 282, 998, 1539, 2147
MspI CCGG 6 cut(s) 1306, 1352, 1604, 1711, 1805, 1981
MspR9I CCNGG 6 cut(s) 25, 467, 1306, 1513, 1605, 1885
MunI CAATTG 2 cut(s) 762, 1097
Mva1269I GAATGC 1 cut(s) 1036
MvaI CCWGG 4 cut(s) 25, 467, 1513, 1885
MvnI CGCG 1 cut(s) 541
NciI CCSGG 2 cut(s) 1306, 1605
NdeI CATATG 1 cut(s) 888
NheI GCTAGC 1 cut(s) 2036
NlaIII CATG 7 cut(s) 90, 520, 629, 739, 833, 1709, 2329
NlaIV GGNNCC 7 cut(s) 358, 471, 1108, 1224, 1620, 1979, 2337
NsiI ATGCAT 2 cut(s) 1036, 2347
NspI RCATGY 1 cut(s) 833
NspV TTCGAA 4 cut(s) 33, 702, 1439, 2172
PaeR7I CTCGAG 1 cut(s) 847
PciSI GCTCTTC 1 cut(s) 2411
PctI GAATGC 1 cut(s) 1036
PdmI GAANNNNTTC 3 cut(s) 1368, 2138, 2285
PfoI TCCNGGA 3 cut(s) 23, 465, 1603
PkrI GCNGC 1 cut(s) 894
Ple19I CGATCG 2 cut(s) 1520, 2164
PleI GAGTC 2 cut(s) 155, 2280
PpsI GAGTC 2 cut(s) 155, 2280
Ppu21I YACGTR 1 cut(s) 1560
PsiI TTATAA 1 cut(s) 149
Psp1406I AACGTT 1 cut(s) 142
Psp6I CCWGG 4 cut(s) 23, 465, 1511, 1883
PspFI CCCAGC 1 cut(s) 797
PspGI CCWGG 4 cut(s) 23, 465, 1511, 1883
PspN4I GGNNCC 7 cut(s) 358, 471, 1108, 1224, 1620, 1979, 2337
PspPI GGNCC 8 cut(s) 90, 265, 356, 622, 1455, 1978, 1983, 2361
PvuI CGATCG 2 cut(s) 1520, 2164
RsaI GTAC 4 cut(s) 249, 484, 1026, 2117
RsaNI GTAC 4 cut(s) 248, 483, 1025, 2116
SapI GCTCTTC 1 cut(s) 2411
SaqAI TTAA 4 cut(s) 282, 998, 1539, 2147
SatI GCNGC 1 cut(s) 893
Sau96I GGNCC 8 cut(s) 90, 265, 356, 622, 1455, 1978, 1983, 2361
ScaI AGTACT 2 cut(s) 249, 484
SchI GAGTC 2 cut(s) 155, 2281
ScrFI CCNGG 6 cut(s) 25, 467, 1306, 1513, 1605, 1885
SduI GDGCHC 1 cut(s) 880
SfaNI GCATC 2 cut(s) 1043, 1351
Sfr274I CTCGAG 1 cut(s) 847
SfuI TTCGAA 4 cut(s) 33, 702, 1439, 2172
SinI GGWCC 8 cut(s) 90, 265, 356, 622, 1455, 1978, 1983, 2361
SlaI CTCGAG 1 cut(s) 847
SmlI CTYRAG 1 cut(s) 847
SmoI CTYRAG 1 cut(s) 847
SnaBI TACGTA 1 cut(s) 1560
SsiI CCGC 8 cut(s) 263, 541, 616, 770, 892, 901, 935, 1992
SspI AATATT 1 cut(s) 1045
SspMI CTAG 5 cut(s) 579, 1068, 1092, 1461, 2037
StyD4I CCNGG 6 cut(s) 23, 465, 1304, 1511, 1603, 1883
TaaI ACNGT 2 cut(s) 1576, 1646
TaiI ACGT 4 cut(s) 145, 1324, 1562, 1733
TaqII GACCGA 1 cut(s) 1281
TatI WGTACW 2 cut(s) 247, 482
TauI GCSGC 1 cut(s) 895
Tru1I TTAA 4 cut(s) 282, 998, 1539, 2147
Tru9I TTAA 4 cut(s) 282, 998, 1539, 2147
TscAI CASTG 1 cut(s) 111
TspGWI ACGGA 4 cut(s) 1042, 1100, 1256, 1331
TspRI CASTG 1 cut(s) 111
VpaK11BI GGWCC 8 cut(s) 90, 265, 356, 622, 1455, 1978, 1983, 2361
XapI RAATTY 9 cut(s) 64, 95, 168, 346, 685, 1367, 2181, 2260, 2377
XceI RCATGY 1 cut(s) 833
XhoI CTCGAG 1 cut(s) 847
XmnI GAANNNNTTC 3 cut(s) 1368, 2138, 2285
XspI CTAG 5 cut(s) 579, 1068, 1092, 1461, 2037
ZrmI AGTACT 2 cut(s) 249, 484
Zsp2I ATGCAT 2 cut(s) 1036, 2347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.