pycom520g00420

aspartic-type endopeptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_520
OG0004874
Physical Location & Seq
Forward (+)
351204 .. 355624
4421 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3834 bp
ATGGATTGTTATTTGAATGGATATTATTCTCTCGAATTTTCAATCAATTTATTTCTAGGTATGTCTAGTGGGGAAGATAGTTGTCTGGCAGATAGTGTAACCACACACACCATATTGCGTGAAAAACACTATTTTACTAACTTCATACCCAAGAAAGCTCCTTTGACAACTTTCTCAGGCTCATCCAACTTGAATGAAGGATACGGAAAGACACGTATCATGTTTTCCAATGGTACAATTCTGACCATTAAGGAGGCACTCTATTCTCCACGTTTCAGAAGAATGTTACTCAGTTTTAGAGATATTCGAGATAATCAATATCATCTTGAAACCACTGAAGATCATGGTTCTGAATTTCTTTGTATCACTTCATATCAATATGGCCAAAAGCATATTCACGAGAAGTTGGAATGCTTGCCAAGTGGGTTACACATCACAACCATTCGCGACATAGAAGCCCACAGTGTGGCCGGCCCCATGCCTGAGTTCCAAGACACTTTATTGCTTTGTCATGACCGTTTGGGACATCTTAGACGTGACATGATGCGCTGTATCCTCAAATCCTCACACGGGCATAAGCTACATCCCTATGTTGGACTCCTACCATGCAAAGCGTGTTCTTTAGGGAAGTTAAATACTCAACCCTCATATACAAAGATTATTGACGATCCCCCTAAATTTCTTCAAAGGATTCAAGGGGACATTTGTGGACTTATCCAACCAACCTGTGAACCATTTAGATACTTTATGGTGTTGGTTGATGCATCGACACGATGGTCACATGTTTGCTTGTTGTCCACACGTAACGCCGTGTTTGCTAAACTTCTAGCTCAAATTATTAAGCTAAGGGCTTACCACCCTGATTATCCAATCAAGTTGATTCAACTAGATAATGCTGGAGAGTTTACGTCACAGACTTTTGATGATTATTTCATGTCAGTAGGGATTGATGTGGAACATCTTGTGCCCTATGTTCACACCCAAAACGGCCTGGGAGAAGCTTTCATAAAGTGCCTTCAATTGATAGCTCGGACTTTGGTTATGAGAACCAAACTGCCGGTATCTGCCTGGGGCTATGCAATATTGCACCCAACTATGTTGGTCCGCCTGAAGCCCACCGCTACCCAACCATATTCACCGTTACAGTTGGTCACTGGATACGAGCCTGACGTCTCGCATTTACAGGTGTTTAGGTGTGCCATTTATGTGCCAATAATGCCGCCCAATTGTTTAATACTTAGAACCCTTGACAGGAGATCGATCTTTACGATACGTTTTGCATATTGTCACTTTGATGAGATAGTCTTCCCGTCGTTAGGGGGAGATAAGCATGCTAATGTTCCCATAGAACGCCACGAATTGTCGTGGTATGCTCCCACTATGTCTCATTTAGATCCCCGCACTGCCCAGTCTGAAACTAAGGTGCGACAAATTATAGATCTTCAGATCATTGCTCAAAGCATGCCGGATGGTTTTAATGATCTAGCAAAGGTGACAAGATCACATATACCCACTACAAACACACCTACAAGGATAGATGTACCCCGTGTATGTCAACAACCCGCCTGGGAAGGCCGAATTGTCCCCGAAGCACCTGCTCTGACTCTAAAGCATGGCAGACCCCTTGGTTCAAAGGATTCACAACCCCGAAATAGGAAAATGACACCAACTAGTAACCCATTTTTGAATCCGACCATTGCTCACTCATCCATTCCAACGCATGAACCTCCCGAGAATCATGAGATTTCGGTCCACTACACAATATTGGATGAGGTTTGGAATAGGAATGAGATGATTGTCGACGATGTATTCTCATACTCAGTAGCTACTGACATCATGCTTAGGGATGACATTGAACCACGTTCCGTCGATGAATGTCGACGTAGAACCGATTGGTCAAATTGGAAACAAGCAATCCAGGTCAAACTTGATTCGCTTGCGAAACATAAGGTATTTGGACCTATCGTTCCTACACCACCACGCGTGAAGCCTATTGGCTACAAGTGGGTTTTTGTGAGGAAGCGTAATGAGAAGAATGAAATAGTGCGATATAAAGCACGCCTCGAGACGTATTCCCCTGTAATGGACGTTATAACGTTCCGCTACCTAATCGGTTTGGTAATTTTCAAAAAACTGAATATGCAGCTTATGGACGTGGTAACCACATATCTCTATGGGGATTTAGATACAGAAATATACATGAAAGTTCCAGAAAGACTTCCATTGATTGGCTCATATAGTTCTAGACCACGAAACACTCTCTCAATTAGGTTGAGGAGCTTACTCTACGGATTGAAACAATCCGAGCTGATGTGGTATAACCGTCTGAGTGAATATTTGACAAGTCAGGGTTATGTGAACAACGAACTATGCCCATGTGTGTTCATAAAGAAGTCACATTTCGGATTTGCAATTGTTGCAGTTTATGTCGATGACATAAATCTCATTGGGACTTTTGCAGAACTTGAGGAAATTGCTGCACACTTGAAATCAGAATTTGAGATGAAGGATCTTGAAGGAAAACTCGATATTGCCTCGCAATCGAACTACACCCAAAATGTGTTACGTCGTTTTAATGAGGATAAAGCGAAGCATTCGAGTACACCCATAATCGTTCGAACTCTAGATGCTCAACGAGATCCTTTCCGTCCAAAGGAAGATGAGGAAGAGATTTTAGAACCCGAAGTTCCTTACTTAAGTGCAATTGAGGCTTTATTGTACTTGGCTCAATGCACTAGACCTGACATCTCCTTCACTGTGAATCTATTGGCAATATACAACAATGCACATACACGCATGCACTGGAATGGTGGTAAAGACATTTTCCGCTACCTCAAAGGTACAACAGATTTGGGCTTGTTCTATACCCGCGAATCTCCCAATGTTGCCACCCTTTATGGTACTCAGATTGATTCTCGCCTTATTGGTTACACATATGTTGGATATCTGTCTGACCCACATAGAGCACGTTCTCAAACGGGTTGTCAAACGCTAGTTGCAACTTCGTCTAACCATGCTAAGATTCTCGCCCTACATGAAGCATCCCGTGAGTGTTTTTGGCTGAGAGAAGTTATGGGACACATTCGAAGCACTAGTGGTCTTACATCAGTTGTTGACTTTCCTATGACGATCTTTCAAGACAATGCAGCATGTATCGAGCAGTTGAAGAAGGGATACATCAAGGGAGACAACACTAAGCACATAGCGCCGAAGTTCGTTTACTCACACCAGCAACAGCAGCATCAGAACATTGAAGTCAAGCAAATCCGTTCCGAGGACAACCTGGCCGATCTCTTCAACAAGTTGTTGCCCAACGCTTGTACTTCTCTTATTTATAAGTTTTGTACTTTTTTTCCTACGATTAGGAGCATTTTTCCCATAGGGTTTTTGCTACCTAATGAGGTTTTAATGAGGCACCCATCTTGGGATGATCATACTCCGTTGAGTTCTCTACCTTCATTCAGTTTGACGTTTTCTATGGGTTTTCCCCATGCCTTGGGTTTTACCATAGTGAAGTTTTGTGAGTTTTACTACAAATGCATACTTCACTCAAATTTGAGACTCGAGATCACCCGTTATGTCGATGACTTCATCAACTATTCTACCTTATCTGAATGTGATTGTGTAAATCCAGATGTGGGAAAGTATCTTATATTCCTATTAGGACTAGATTACCTATTAAATATTGTAATCCTAAAGGGAAAATTTTTCACATTTCCTACTACTATAAATAAAGCTCTCTTCCCCTTATTGTGGCCGGCTCCTCTTTCGTTCAATCAAATAGGTCTACAACAGCTACAACTATTTTCAAAAACTACGTTAATTAATTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 rve

Protein Analysis

1278

Amino Acids

146.51

Weight (kDa)

8.24

Isoelectric Point (pI)

47.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pol_BBD PF22936 29 - 107 8.5e-07 Pol polyprotein, beta-barrel domain
gag_pre-integrs PF13976 146 - 210 6.9e-06 GAG-pre-integrase domain
rve PF00665 229 - 324 1.4e-08 Integrase core domain
RVT_2 PF07727 655 - 839 3.2e-26 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2093, 3336
AarI CACCTGC 1 cut(s) 1603
AasI GACNNNNNNGTC 1 cut(s) 775
AatII GACGTC 1 cut(s) 1173
Acc36I ACCTGC 1 cut(s) 1603
AccB1I GGYRCC 1 cut(s) 3414
AccB7I CCANNNNNTGG 3 cut(s) 466, 2228, 3496
AccI GTMKAC 3 cut(s) 1800, 1879, 3787
AccII CGCG 3 cut(s) 447, 1983, 2871
AciI CCGC 8 cut(s) 1105, 1119, 1220, 1399, 1563, 2101, 2827, 2869
AclI AACGTT 1 cut(s) 2096
AclWI GGATC 4 cut(s) 662, 1388, 2517, 2633
AcoI YGGCCR 4 cut(s) 382, 468, 3285, 3755
AcsI RAATTY 6 cut(s) 35, 353, 677, 2495, 3553, 3704
AcuI CTGAAG 3 cut(s) 357, 1130, 1427
AcyI GRCGYC 1 cut(s) 1170
AdeI CACNNNGTG 1 cut(s) 466
AfaI GTAC 8 cut(s) 235, 1542, 2602, 2720, 2842, 2902, 3322, 3346
AflII CTTAAG 1 cut(s) 2695
AflIII ACRYGT 4 cut(s) 212, 781, 800, 1981
AhlI ACTAGT 2 cut(s) 1670, 3092
AjiI CACGTC 2 cut(s) 536, 2155
AjnI CCWGG 5 cut(s) 990, 1067, 1565, 1917, 3282
AjuI GAANNNNNNNTTGG 2 cut(s) 378, 410
AloI GAACNNNNNNTCC 2 cut(s) 1950, 1982
Alw21I GWGCWC 1 cut(s) 2968
Alw26I GTCTC 5 cut(s) 1177, 1389, 2060, 3180, 3553
AlwI GGATC 4 cut(s) 662, 1388, 2517, 2633
Ama87I CYCGRG 3 cut(s) 1730, 2063, 3563
AoxI GGCC 7 cut(s) 382, 468, 472, 988, 1573, 3285, 3755
ApeKI GCWGC 4 cut(s) 2143, 2477, 3146, 3238
ApoI RAATTY 6 cut(s) 35, 353, 677, 2495, 3553, 3704
ArsI GACNNNNNNTTYG 2 cut(s) 2968, 3000
AseI ATTAAT 1 cut(s) 3825
Asp700I GAANNNNTTC 1 cut(s) 2217
AspLEI GCGC 2 cut(s) 549, 3208
AspS9I GGNCC 4 cut(s) 473, 1102, 1750, 1958
AsuHPI GGTGA 3 cut(s) 1128, 1504, 3562
AsuII TTCGAA 2 cut(s) 2617, 3085
AvaI CYCGRG 3 cut(s) 1730, 2063, 3563
AvaII GGWCC 3 cut(s) 1102, 1750, 1958
BaeGI GKGCMC 1 cut(s) 969
BalI TGGCCA 1 cut(s) 384
BanI GGYRCC 1 cut(s) 3414
BarI GAAGNNNNNNTAC 4 cut(s) 3158, 3190, 3203, 3235
BauI CACGAG 1 cut(s) 398
BbsI GAAGAC 1 cut(s) 1297
Bbv12I GWGCWC 1 cut(s) 2968
BbvI GCAGC 4 cut(s) 2155, 2464, 3158, 3250
BccI CCATC 3 cut(s) 768, 1463, 3427
BceAI ACGGC 2 cut(s) 794, 1003
BcgI CGANNNNNNTGC 2 cut(s) 1577, 1611
BciT130I CCWGG 5 cut(s) 992, 1069, 1567, 1919, 3284
BciVI GTATCC 4 cut(s) 194, 563, 1151, 3167
BclI TGATCA 1 cut(s) 3430
BcoDI GTCTC 5 cut(s) 1177, 1389, 2060, 3180, 3553
BcuI ACTAGT 2 cut(s) 1670, 3092
BfoI RGCGCY 1 cut(s) 3209
BfrI CTTAAG 1 cut(s) 2695
BfuAI ACCTGC 1 cut(s) 1603
BfuI GTATCC 4 cut(s) 194, 563, 1151, 3167
BglII AGATCT 1 cut(s) 1438
BisI GCNGC 5 cut(s) 1220, 2144, 2478, 3147, 3239
BlsI GCNGC 5 cut(s) 1221, 2145, 2479, 3148, 3240
Bme1390I CCNGG 5 cut(s) 992, 1069, 1567, 1919, 3284
Bme18I GGWCC 3 cut(s) 1102, 1750, 1958
BmeT110I CYCGRG 3 cut(s) 1730, 2063, 3563
BmgBI CACGTC 2 cut(s) 536, 2155
BmgT120I GGNCC 4 cut(s) 473, 1102, 1750, 1958
BmiI GGNNCC 3 cut(s) 475, 3416, 3762
BmrFI CCNGG 5 cut(s) 992, 1069, 1567, 1919, 3284
BmrI ACTGGG 1 cut(s) 1402
BmsI GCATC 6 cut(s) 534, 751, 773, 2617, 3050, 3250
BmuI ACTGGG 1 cut(s) 1402
BpiI GAAGAC 1 cut(s) 1297
BpmI CTGGAG 1 cut(s) 918
Bpu10I CCTNAGC 2 cut(s) 845, 1841
Bpu14I TTCGAA 2 cut(s) 2617, 3085
BpuEI CTTGAG 1 cut(s) 2486
Bsa29I ATCGAT 1 cut(s) 1259
BsaAI YACGTR 2 cut(s) 215, 803
BsaHI GRCGYC 1 cut(s) 1170
BsaJI CCNNGG 6 cut(s) 991, 1068, 1566, 1624, 3273, 3495
Bse118I RCCGGY 3 cut(s) 470, 1057, 3757
Bse1I ACTGG 3 cut(s) 1159, 1408, 2807
Bse3DI GCAATG 2 cut(s) 1449, 1695
BseBI CCWGG 5 cut(s) 992, 1069, 1567, 1919, 3284
BseCI ATCGAT 1 cut(s) 1259
BseDI CCNNGG 6 cut(s) 991, 1068, 1566, 1624, 3273, 3495
BseGI GGATG 8 cut(s) 182, 583, 1474, 1706, 1774, 1852, 3041, 3433
BseMI GCAATG 2 cut(s) 1449, 1695
BseMII CTCAG 7 cut(s) 189, 304, 474, 1833, 2318, 2918, 3053
BseNI ACTGG 3 cut(s) 1159, 1408, 2807
BseRI GAGGAG 2 cut(s) 2290, 3753
BseSI GKGCMC 1 cut(s) 969
BseXI GCAGC 4 cut(s) 2155, 2464, 3158, 3250
BsgI GTGCAG 1 cut(s) 2463
Bsh1236I CGCG 3 cut(s) 447, 1983, 2871
BshFI GGCC 7 cut(s) 384, 470, 474, 990, 1575, 3287, 3757
BshNI GGYRCC 1 cut(s) 3414
BshVI ATCGAT 1 cut(s) 1259
BsiHKAI GWGCWC 1 cut(s) 2968
BsiHKCI CYCGRG 3 cut(s) 1730, 2063, 3563
BsiSI CCGG 4 cut(s) 471, 1058, 1466, 3758
BslFI GGGAC 5 cut(s) 537, 713, 1568, 2464, 3090
BsmAI GTCTC 5 cut(s) 1177, 1389, 2060, 3180, 3553
BsmBI CGTCTC 2 cut(s) 1177, 2060
BsmFI GGGAC 5 cut(s) 537, 713, 1568, 2464, 3090
BsmI GAATGC 2 cut(s) 416, 2593
BsnI GGCC 7 cut(s) 384, 470, 474, 990, 1575, 3287, 3757
BsoBI CYCGRG 3 cut(s) 1730, 2063, 3563
Bsp119I TTCGAA 2 cut(s) 2617, 3085
Bsp1286I GDGCHC 2 cut(s) 969, 2968
Bsp68I TCGCGA 1 cut(s) 447
BspACI CCGC 8 cut(s) 1105, 1119, 1220, 1399, 1563, 2101, 2827, 2869
BspANI GGCC 7 cut(s) 384, 470, 474, 990, 1575, 3287, 3757
BspCNI CTCAG 7 cut(s) 188, 303, 475, 1832, 2319, 2917, 3054
BspDI ATCGAT 1 cut(s) 1259
BspFNI CGCG 3 cut(s) 447, 1983, 2871
BspHI TCATGA 2 cut(s) 511, 1738
BspLI GGNNCC 3 cut(s) 475, 3416, 3762
BspMI ACCTGC 1 cut(s) 1603
BspPI GGATC 4 cut(s) 662, 1388, 2517, 2633
BspT104I TTCGAA 2 cut(s) 2617, 3085
BspT107I GGYRCC 1 cut(s) 3414
BspTI CTTAAG 1 cut(s) 2695
BsrDI GCAATG 2 cut(s) 1449, 1695
BsrFI RCCGGY 3 cut(s) 470, 1057, 3757
BsrI ACTGG 3 cut(s) 1159, 1408, 2807
BssAI RCCGGY 3 cut(s) 470, 1057, 3757
BssECI CCNNGG 6 cut(s) 991, 1068, 1566, 1624, 3273, 3495
BssNI GRCGYC 1 cut(s) 1170
BssSI CACGAG 1 cut(s) 398
BssT1I CCWWGG 2 cut(s) 1624, 3495
Bst2BI CACGAG 1 cut(s) 398
Bst2UI CCWGG 5 cut(s) 992, 1069, 1567, 1919, 3284
Bst4CI ACNGT 6 cut(s) 464, 518, 1140, 1146, 2324, 2758
Bst6I CTCTTC 3 cut(s) 2661, 3299, 3746
BstACI GRCGYC 1 cut(s) 1170
BstAFI CTTAAG 1 cut(s) 2695
BstAPI GCANNNNNTGC 1 cut(s) 2417
BstBAI YACGTR 2 cut(s) 215, 803
BstBI TTCGAA 2 cut(s) 2617, 3085
BstC8I GCNNGC 8 cut(s) 416, 472, 1332, 1463, 1938, 2059, 2798, 3759
BstEII GGTNACC 1 cut(s) 2158
BstF5I GGATG 8 cut(s) 182, 583, 1474, 1706, 1774, 1852, 3041, 3433
BstFNI CGCG 3 cut(s) 447, 1983, 2871
BstH2I RGCGCY 1 cut(s) 3209
BstHHI GCGC 2 cut(s) 549, 3208
BstMAI GTCTC 5 cut(s) 1177, 1389, 2060, 3180, 3553
BstMWI GCNNNNNNNGC 6 cut(s) 815, 1216, 2417, 2708, 3205, 3238
BstNI CCWGG 5 cut(s) 992, 1069, 1567, 1919, 3284
BstNSI RCATGY 5 cut(s) 785, 1334, 1465, 2800, 3153
BstPI GGTNACC 1 cut(s) 2158
BstSCI CCNGG 5 cut(s) 990, 1067, 1565, 1917, 3282
BstSLI GKGCMC 1 cut(s) 969
BstUI CGCG 3 cut(s) 447, 1983, 2871
BstV1I GCAGC 4 cut(s) 2155, 2464, 3158, 3250
BstV2I GAAGAC 1 cut(s) 1297
BstX2I RGATCY 4 cut(s) 1393, 1438, 2509, 2638
BstYI RGATCY 4 cut(s) 1393, 1438, 2509, 2638
Bsu15I ATCGAT 1 cut(s) 1259
BsuI GTATCC 4 cut(s) 194, 563, 1151, 3167
BsuRI GGCC 7 cut(s) 384, 470, 474, 990, 1575, 3287, 3757
BsuTUI ATCGAT 1 cut(s) 1259
BtrI CACGTC 2 cut(s) 536, 2155
BtsCI GGATG 8 cut(s) 182, 583, 1474, 1706, 1774, 1852, 3041, 3433
BtsI GCAGTG 1 cut(s) 1401
BtsIMutI CAGTG 6 cut(s) 333, 469, 1152, 1401, 2754, 2800
BtuMI TCGCGA 1 cut(s) 447
BveI ACCTGC 1 cut(s) 1603
Cac8I GCNNGC 8 cut(s) 416, 472, 1332, 1463, 1938, 2059, 2798, 3759
CciI TCATGA 2 cut(s) 511, 1738
CfoI GCGC 2 cut(s) 549, 3208
Cfr10I RCCGGY 3 cut(s) 470, 1057, 3757
Cfr13I GGNCC 4 cut(s) 473, 1102, 1750, 1958
ClaI ATCGAT 1 cut(s) 1259
Csp6I GTAC 8 cut(s) 234, 1541, 2601, 2719, 2841, 2901, 3321, 3345
CviQI GTAC 8 cut(s) 234, 1541, 2601, 2719, 2841, 2901, 3321, 3345
DraIII CACNNNGTG 1 cut(s) 466
DrdI GACNNNNNNGTC 1 cut(s) 775
DseDI GACNNNNNNGTC 1 cut(s) 775
EaeI YGGCCR 4 cut(s) 382, 468, 3285, 3755
Eam1104I CTCTTC 3 cut(s) 2661, 3299, 3746
EarI CTCTTC 3 cut(s) 2661, 3299, 3746
EciI GGCGGA 1 cut(s) 1094
Eco130I CCWWGG 2 cut(s) 1624, 3495
Eco32I GATATC 1 cut(s) 2945
Eco47I GGWCC 3 cut(s) 1102, 1750, 1958
Eco57I CTGAAG 3 cut(s) 357, 1130, 1427
Eco88I CYCGRG 3 cut(s) 1730, 2063, 3563
Eco91I GGTNACC 1 cut(s) 2158
EcoO65I GGTNACC 1 cut(s) 2158
EcoRII CCWGG 5 cut(s) 990, 1067, 1565, 1917, 3282
EcoRV GATATC 1 cut(s) 2945
EcoT14I CCWWGG 2 cut(s) 1624, 3495
EcoT22I ATGCAT 2 cut(s) 766, 3542
ErhI CCWWGG 2 cut(s) 1624, 3495
Esp3I CGTCTC 2 cut(s) 1177, 2060
FaqI GGGAC 5 cut(s) 537, 713, 1568, 2464, 3090
FauI CCCGC 3 cut(s) 1406, 1570, 2876
FauNDI CATATG 1 cut(s) 2935
FbaI TGATCA 1 cut(s) 3430
FblI GTMKAC 3 cut(s) 1800, 1879, 3787
Fnu4HI GCNGC 5 cut(s) 1220, 2144, 2478, 3147, 3239
FokI GGATG 8 cut(s) 169, 570, 1481, 1693, 1781, 1859, 3028, 3440
FseI GGCCGGCC 1 cut(s) 474
Fsp4HI GCNGC 5 cut(s) 1220, 2144, 2478, 3147, 3239
GlaI GCGC 2 cut(s) 548, 3207
GluI GCNGC 5 cut(s) 1220, 2144, 2478, 3147, 3239
GsuI CTGGAG 1 cut(s) 918
HaeII RGCGCY 1 cut(s) 3209
HaeIII GGCC 7 cut(s) 384, 470, 474, 990, 1575, 3287, 3757
HapII CCGG 4 cut(s) 471, 1058, 1466, 3758
HhaI GCGC 2 cut(s) 549, 3208
Hin1I GRCGYC 1 cut(s) 1170
Hin6I GCGC 2 cut(s) 547, 3206
HinP1I GCGC 2 cut(s) 547, 3206
HincII GTYRAC 4 cut(s) 1556, 1801, 1880, 3115
HindII GTYRAC 4 cut(s) 1556, 1801, 1880, 3115
HindIII AAGCTT 1 cut(s) 999
HpaII CCGG 4 cut(s) 471, 1058, 1466, 3758
HphI GGTGA 3 cut(s) 1128, 1504, 3562
Hpy99I CGWCG 5 cut(s) 1315, 1805, 1871, 1884, 2571
HpyAV CCTTC 8 cut(s) 191, 1025, 1565, 2500, 2510, 2761, 3163, 3465
HpyCH4III ACNGT 6 cut(s) 464, 518, 1140, 1146, 2324, 2758
HpyF10VI GCNNNNNNNGC 6 cut(s) 815, 1216, 2417, 2708, 3205, 3238
Hsp92I GRCGYC 1 cut(s) 1170
HspAI GCGC 2 cut(s) 547, 3206
KroI GCCGGC 2 cut(s) 470, 3757
KroNI GCCGGC 2 cut(s) 472, 3759
Ksp22I TGATCA 1 cut(s) 3430
LmnI GCTCC 5 cut(s) 163, 1378, 2277, 3366, 3766
Lsp1109I GCAGC 4 cut(s) 2155, 2464, 3158, 3250
LweI GCATC 6 cut(s) 534, 751, 773, 2617, 3050, 3250
MfeI CAATTG 4 cut(s) 1019, 1225, 2412, 2703
MflI RGATCY 4 cut(s) 1393, 1438, 2509, 2638
MhlI GDGCHC 2 cut(s) 969, 2968
MlsI TGGCCA 1 cut(s) 384
MluI ACGCGT 1 cut(s) 1981
MluNI TGGCCA 1 cut(s) 384
MlyI GAGTC 3 cut(s) 591, 1597, 3555
MmeI TCCRAC 7 cut(s) 210, 387, 574, 742, 1715, 1739, 2920
Mox20I TGGCCA 1 cut(s) 384
Mph1103I ATGCAT 2 cut(s) 766, 3542
MroNI GCCGGC 2 cut(s) 470, 3757
MroXI GAANNNNTTC 1 cut(s) 2217
MscI TGGCCA 1 cut(s) 384
MslI CAYNNNNRTG 5 cut(s) 588, 1205, 1293, 1335, 2805
Msp20I TGGCCA 1 cut(s) 384
MspCI CTTAAG 1 cut(s) 2695
MspI CCGG 4 cut(s) 471, 1058, 1466, 3758
MspR9I CCNGG 5 cut(s) 992, 1069, 1567, 1919, 3284
MunI CAATTG 4 cut(s) 1019, 1225, 2412, 2703
Mva1269I GAATGC 2 cut(s) 416, 2593
MvaI CCWGG 5 cut(s) 992, 1069, 1567, 1919, 3284
MvnI CGCG 3 cut(s) 447, 1983, 2871
MwoI GCNNNNNNNGC 6 cut(s) 815, 1216, 2417, 2708, 3205, 3238
NaeI GCCGGC 2 cut(s) 472, 3759
NdeI CATATG 1 cut(s) 2935
NgoMIV GCCGGC 2 cut(s) 470, 3757
NlaIV GGNNCC 3 cut(s) 475, 3416, 3762
NmuCI GTSAC 7 cut(s) 536, 777, 909, 1150, 1286, 1492, 2394
NruI TCGCGA 1 cut(s) 447
NsiI ATGCAT 2 cut(s) 766, 3542
NspI RCATGY 5 cut(s) 785, 1334, 1465, 2800, 3153
NspV TTCGAA 2 cut(s) 2617, 3085
PacI TTAATTAA 1 cut(s) 3825
PaeI GCATGC 3 cut(s) 1334, 1465, 2800
PaeR7I CTCGAG 2 cut(s) 2063, 3563
PagI TCATGA 2 cut(s) 511, 1738
PaqCI CACCTGC 1 cut(s) 1603
PciI ACATGT 1 cut(s) 781
PcsI WCGNNNNNNNCGW 2 cut(s) 1167, 1265
PctI GAATGC 2 cut(s) 416, 2593
PdiI GCCGGC 2 cut(s) 472, 3759
PdmI GAANNNNTTC 1 cut(s) 2217
PflMI CCANNNNNTGG 3 cut(s) 466, 2228, 3496
PkrI GCNGC 5 cut(s) 1221, 2145, 2479, 3148, 3240
PleI GAGTC 3 cut(s) 591, 1597, 3555
PpsI GAGTC 3 cut(s) 591, 1597, 3555
Ppu21I YACGTR 2 cut(s) 215, 803
PscI ACATGT 1 cut(s) 781
PshBI ATTAAT 1 cut(s) 3825
PsiI TTATAA 2 cut(s) 2093, 3336
Psp1406I AACGTT 1 cut(s) 2096
Psp6I CCWGG 5 cut(s) 990, 1067, 1565, 1917, 3282
PspEI GGTNACC 1 cut(s) 2158
PspGI CCWGG 5 cut(s) 990, 1067, 1565, 1917, 3282
PspN4I GGNNCC 3 cut(s) 475, 3416, 3762
PspPI GGNCC 4 cut(s) 473, 1102, 1750, 1958
PsuI RGATCY 4 cut(s) 1393, 1438, 2509, 2638
RigI GGCCGGCC 1 cut(s) 474
RruI TCGCGA 1 cut(s) 447
RsaI GTAC 8 cut(s) 235, 1542, 2602, 2720, 2842, 2902, 3322, 3346
RsaNI GTAC 8 cut(s) 234, 1541, 2601, 2719, 2841, 2901, 3321, 3345
RseI CAYNNNNRTG 5 cut(s) 588, 1205, 1293, 1335, 2805
SalI GTCGAC 2 cut(s) 1799, 1878
SatI GCNGC 5 cut(s) 1220, 2144, 2478, 3147, 3239
Sau96I GGNCC 4 cut(s) 473, 1102, 1750, 1958
SchI GAGTC 3 cut(s) 591, 1597, 3555
ScrFI CCNGG 5 cut(s) 992, 1069, 1567, 1919, 3284
SduI GDGCHC 2 cut(s) 969, 2968
SfaNI GCATC 6 cut(s) 534, 751, 773, 2617, 3050, 3250
Sfr274I CTCGAG 2 cut(s) 2063, 3563
SfuI TTCGAA 2 cut(s) 2617, 3085
SinI GGWCC 3 cut(s) 1102, 1750, 1958
SlaI CTCGAG 2 cut(s) 2063, 3563
SmiMI CAYNNNNRTG 5 cut(s) 588, 1205, 1293, 1335, 2805
SmlI CTYRAG 4 cut(s) 2063, 2465, 2695, 3563
SmoI CTYRAG 4 cut(s) 2063, 2465, 2695, 3563
SpeI ACTAGT 2 cut(s) 1670, 3092
SphI GCATGC 3 cut(s) 1334, 1465, 2800
SsiI CCGC 8 cut(s) 1105, 1119, 1220, 1399, 1563, 2101, 2827, 2869
SspI AATATT 4 cut(s) 1083, 1764, 2336, 3685
StyD4I CCNGG 5 cut(s) 990, 1067, 1565, 1917, 3282
StyI CCWWGG 2 cut(s) 1624, 3495
TaaI ACNGT 6 cut(s) 464, 518, 1140, 1146, 2324, 2758
TaqII GACCGA 1 cut(s) 1738
TatI WGTACW 4 cut(s) 2600, 2718, 3320, 3344
TauI GCSGC 1 cut(s) 1222
TscAI CASTG 6 cut(s) 340, 469, 1159, 1408, 2761, 2807
TseFI GTSAC 7 cut(s) 536, 777, 909, 1150, 1286, 1492, 2394
TseI GCWGC 4 cut(s) 2143, 2477, 3146, 3238
Tsp45I GTSAC 7 cut(s) 536, 777, 909, 1150, 1286, 1492, 2394
TspGWI ACGGA 6 cut(s) 219, 1855, 2304, 2636, 3257, 3429
TspRI CASTG 6 cut(s) 340, 469, 1159, 1408, 2761, 2807
Van91I CCANNNNNTGG 3 cut(s) 466, 2228, 3496
Vha464I CTTAAG 1 cut(s) 2695
VpaK11BI GGWCC 3 cut(s) 1102, 1750, 1958
VspI ATTAAT 1 cut(s) 3825
XapI RAATTY 6 cut(s) 35, 353, 677, 2495, 3553, 3704
XbaI TCTAGA 2 cut(s) 2243, 2623
XceI RCATGY 5 cut(s) 785, 1334, 1465, 2800, 3153
XhoI CTCGAG 2 cut(s) 2063, 3563
XmiI GTMKAC 3 cut(s) 1800, 1879, 3787
XmnI GAANNNNTTC 1 cut(s) 2217
ZraI GACGTC 1 cut(s) 1171
Zsp2I ATGCAT 2 cut(s) 766, 3542
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.