RchiOBHm_Chr2g0150891

transposition, RNA-mediated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0004874
Physical Location & Seq
Forward (+)
68565408 .. 68566246
839 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ52014

Sequence Viewer

Length: 615 bp
ATGGGCTTGTTCTATCCCTATAGAGAGATGATGGATTCGGACCCATCAAGTGCCAGGGATGCCACACATGGTGGACCGCGTTCCCTATCCCCAATCCAAAACGATATAAGTGTTTTGGAAGGTTTTGCTGATGCTGGGTACCTCTCTGACCCACACAAAGGTCGCTCCCAAACTGGTTATGTTTTCACTATGGGAAAGACCGCGATATCTTGGAGGTCTACGAAGCAGACTTTAGTCGCTACTTCTTCGAATCATGCAGAGATTATTGCTCTTCACGAAGCAGTCCGTGAATGTATATGGCTTCGATCCATAGTTACGCATGTTCGAAACAATTGTGGTTTGAAGTCTACCACAGATGAGCCAACGAGCATTTATGAGGATAATGCTGCTTGCATTGAACAAATGAAGCAAGGCTACATCAAAGGCGACAACACCAAGCATATATCGCCTAAATTCTTCTACAATCAGCAACAACAGAAGCTCCTCAAGATCAAAGTGAACCAAGTTCGATCTGAGGACAATGTGGCAGACTTATTTACTAAGTCATTGCCCAAATCCACATTCGAGAAACATGTGGCAAGAATTGGCTTGCGGAAGTTATCTAAACTCCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

204

Amino Acids

22.92

Weight (kDa)

9.2

Isoelectric Point (pI)

48.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 138
AccB1I GGYRCC 1 cut(s) 138
AccI GTMKAC 2 cut(s) 218, 347
AccII CGCG 2 cut(s) 79, 203
AciI CCGC 3 cut(s) 77, 201, 592
AclWI GGATC 1 cut(s) 300
AcsI RAATTY 1 cut(s) 452
AdeI CACNNNGTG 1 cut(s) 71
AfaI GTAC 1 cut(s) 140
AfiI CCNNNNNNNGG 1 cut(s) 158
AflIII ACRYGT 1 cut(s) 571
AgsI TTSAA 2 cut(s) 343, 398
AjnI CCWGG 1 cut(s) 53
AjuI GAANNNNNNNTTGG 2 cut(s) 545, 577
AluBI AGCT 1 cut(s) 481
AluI AGCT 1 cut(s) 481
AlwI GGATC 1 cut(s) 300
ApeKI GCWGC 1 cut(s) 386
ApoI RAATTY 1 cut(s) 452
Asp718I GGTACC 1 cut(s) 138
AspS9I GGNCC 2 cut(s) 40, 74
AsuII TTCGAA 2 cut(s) 248, 325
AvaII GGWCC 2 cut(s) 40, 74
BanI GGYRCC 1 cut(s) 138
BarI GAAGNNNNNNTAC 2 cut(s) 398, 430
BbvI GCAGC 1 cut(s) 373
BccI CCATC 2 cut(s) 25, 52
BciT130I CCWGG 1 cut(s) 55
BfmI CTRYAG 1 cut(s) 19
BisI GCNGC 1 cut(s) 387
BlsI GCNGC 1 cut(s) 388
Bme1390I CCNGG 1 cut(s) 55
Bme18I GGWCC 2 cut(s) 40, 74
BmgT120I GGNCC 2 cut(s) 40, 74
BmiI GGNNCC 2 cut(s) 42, 140
BmrFI CCNGG 1 cut(s) 55
BmsI GCATC 2 cut(s) 49, 121
BoxI GACNNNNGTC 1 cut(s) 233
Bpu14I TTCGAA 2 cut(s) 248, 325
BpuEI CTTGAG 1 cut(s) 470
BsaJI CCNNGG 1 cut(s) 54
BsaXI ACNNNNNCTCC 2 cut(s) 465, 495
Bsc4I CCNNNNNNNGG 1 cut(s) 158
Bse1I ACTGG 1 cut(s) 178
Bse3DI GCAATG 1 cut(s) 545
BseBI CCWGG 1 cut(s) 55
BseDI CCNNGG 1 cut(s) 54
BseGI GGATG 1 cut(s) 64
BseLI CCNNNNNNNGG 1 cut(s) 158
BseMI GCAATG 1 cut(s) 545
BseMII CTCAG 1 cut(s) 504
BseNI ACTGG 1 cut(s) 178
BseRI GAGGAG 1 cut(s) 473
BseXI GCAGC 1 cut(s) 373
BseYI CCCAGC 1 cut(s) 134
Bsh1236I CGCG 2 cut(s) 79, 203
BshNI GGYRCC 1 cut(s) 138
BslI CCNNNNNNNGG 1 cut(s) 158
Bsp119I TTCGAA 2 cut(s) 248, 325
Bsp143I GATC 3 cut(s) 305, 489, 509
BspACI CCGC 3 cut(s) 77, 201, 592
BspCNI CTCAG 1 cut(s) 505
BspFNI CGCG 2 cut(s) 79, 203
BspLI GGNNCC 2 cut(s) 42, 140
BspPI GGATC 1 cut(s) 300
BspQI GCTCTTC 1 cut(s) 276
BspT104I TTCGAA 2 cut(s) 248, 325
BspT107I GGYRCC 1 cut(s) 138
BsrDI GCAATG 1 cut(s) 545
BsrI ACTGG 1 cut(s) 178
BssECI CCNNGG 1 cut(s) 54
BssMI GATC 3 cut(s) 305, 489, 509
Bst2UI CCWGG 1 cut(s) 55
Bst6I CTCTTC 1 cut(s) 276
BstBI TTCGAA 2 cut(s) 248, 325
BstC8I GCNNGC 2 cut(s) 391, 590
BstDEI CTNAG 2 cut(s) 513, 540
BstF5I GGATG 1 cut(s) 64
BstFNI CGCG 2 cut(s) 79, 203
BstKTI GATC 3 cut(s) 308, 492, 512
BstMBI GATC 3 cut(s) 305, 489, 509
BstMWI GCNNNNNNNGC 2 cut(s) 59, 445
BstNI CCWGG 1 cut(s) 55
BstNSI RCATGY 2 cut(s) 323, 575
BstPAI GACNNNNGTC 1 cut(s) 233
BstSCI CCNGG 1 cut(s) 53
BstSFI CTRYAG 1 cut(s) 19
BstUI CGCG 2 cut(s) 79, 203
BstV1I GCAGC 1 cut(s) 373
BtsCI GGATG 1 cut(s) 64
Cac8I GCNNGC 2 cut(s) 391, 590
Cfr13I GGNCC 2 cut(s) 40, 74
Csp6I GTAC 1 cut(s) 139
CviAII CATG 5 cut(s) 68, 254, 320, 572, 612
CviJI RGCY 6 cut(s) 6, 301, 361, 414, 481, 588
CviKI_1 RGCY 6 cut(s) 6, 301, 361, 414, 481, 588
CviQI GTAC 1 cut(s) 139
DdeI CTNAG 2 cut(s) 513, 540
DpnI GATC 3 cut(s) 307, 491, 511
DpnII GATC 3 cut(s) 305, 489, 509
DraIII CACNNNGTG 1 cut(s) 71
Eam1104I CTCTTC 1 cut(s) 276
EarI CTCTTC 1 cut(s) 276
Eco32I GATATC 1 cut(s) 207
Eco47I GGWCC 2 cut(s) 40, 74
EcoRII CCWGG 1 cut(s) 53
EcoRV GATATC 1 cut(s) 207
FaeI CATG 5 cut(s) 71, 257, 323, 575, 615
FatI CATG 5 cut(s) 67, 253, 319, 571, 611
FblI GTMKAC 2 cut(s) 218, 347
Fnu4HI GCNGC 1 cut(s) 387
FokI GGATG 1 cut(s) 71
Fsp4HI GCNGC 1 cut(s) 387
GluI GCNGC 1 cut(s) 387
GsaI CCCAGC 1 cut(s) 138
Hin1II CATG 5 cut(s) 71, 257, 323, 575, 615
HinfI GANTC 2 cut(s) 35, 250
Hpy166II GTNNAC 4 cut(s) 74, 219, 348, 499
Hpy188I TCNGA 3 cut(s) 40, 148, 514
Hpy188III TCNNGA 3 cut(s) 275, 487, 565
Hpy8I GTNNAC 4 cut(s) 74, 219, 348, 499
HpyAV CCTTC 1 cut(s) 113
HpyCH4V TGCA 2 cut(s) 257, 393
HpyF10VI GCNNNNNNNGC 2 cut(s) 59, 445
HpyF3I CTNAG 2 cut(s) 513, 540
Hsp92II CATG 5 cut(s) 71, 257, 323, 575, 615
KpnI GGTACC 1 cut(s) 142
Kzo9I GATC 3 cut(s) 305, 489, 509
LguI GCTCTTC 1 cut(s) 276
LmnI GCTCC 2 cut(s) 170, 486
LpnPI CCDG 4 cut(s) 40, 67, 120, 159
Lsp1109I GCAGC 1 cut(s) 373
LweI GCATC 2 cut(s) 49, 121
MaeIII GTNAC 1 cut(s) 313
MalI GATC 3 cut(s) 307, 491, 511
MboI GATC 3 cut(s) 305, 489, 509
MboII GAAGA 3 cut(s) 237, 263, 448
MfeI CAATTG 1 cut(s) 331
MluCI AATT 3 cut(s) 331, 452, 582
MnlI CCTC 5 cut(s) 152, 207, 370, 494, 508
MspR9I CCNGG 1 cut(s) 55
MunI CAATTG 1 cut(s) 331
MvaI CCWGG 1 cut(s) 55
MvnI CGCG 2 cut(s) 79, 203
MwoI GCNNNNNNNGC 2 cut(s) 59, 445
NdeII GATC 3 cut(s) 305, 489, 509
NlaIII CATG 5 cut(s) 71, 257, 323, 575, 615
NlaIV GGNNCC 2 cut(s) 42, 140
NspI RCATGY 2 cut(s) 323, 575
NspV TTCGAA 2 cut(s) 248, 325
PciI ACATGT 1 cut(s) 571
PciSI GCTCTTC 1 cut(s) 276
PfeI GAWTC 2 cut(s) 35, 250
PkrI GCNGC 1 cut(s) 388
PscI ACATGT 1 cut(s) 571
PshAI GACNNNNGTC 1 cut(s) 233
Psp6I CCWGG 1 cut(s) 53
PspFI CCCAGC 1 cut(s) 134
PspGI CCWGG 1 cut(s) 53
PspN4I GGNNCC 2 cut(s) 42, 140
PspPI GGNCC 2 cut(s) 40, 74
RsaI GTAC 1 cut(s) 140
RsaNI GTAC 1 cut(s) 139
SapI GCTCTTC 1 cut(s) 276
SatI GCNGC 1 cut(s) 387
Sau3AI GATC 3 cut(s) 305, 489, 509
Sau96I GGNCC 2 cut(s) 40, 74
ScrFI CCNGG 1 cut(s) 55
SetI ASST 5 cut(s) 124, 144, 163, 218, 483
SfaNI GCATC 2 cut(s) 49, 121
SfcI CTRYAG 1 cut(s) 19
SfuI TTCGAA 2 cut(s) 248, 325
SinI GGWCC 2 cut(s) 40, 74
SmlI CTYRAG 1 cut(s) 485
SmoI CTYRAG 1 cut(s) 485
Sse9I AATT 3 cut(s) 331, 452, 582
SsiI CCGC 3 cut(s) 77, 201, 592
StyD4I CCNGG 1 cut(s) 53
TaqI TCGA 5 cut(s) 248, 304, 325, 508, 564
TasI AATT 3 cut(s) 331, 452, 582
TfiI GAWTC 2 cut(s) 35, 250
TseI GCWGC 1 cut(s) 386
TspDTI ATGAA 1 cut(s) 419
TspGWI ACGGA 1 cut(s) 275
VpaK11BI GGWCC 2 cut(s) 40, 74
XapI RAATTY 1 cut(s) 452
XceI RCATGY 2 cut(s) 323, 575
XmiI GTMKAC 2 cut(s) 218, 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.