RchiOBHm_Chr1g0367381

aspartic-type endopeptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0004874
Physical Location & Seq
Reverse (-)
57695194 .. 57697704
2511 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ59184

Sequence Viewer

Length: 2394 bp
ATGGGTCCTCAACGAAGGATGGGCATATATGTCGGTTATGAATCCCCATCCATTATACGCTTTTTAGAGCCCTTGACAGGCGATCTCTTTACCGCTAGATTTGCGGATTGTCACTTTGATGAGACAGTCTTCCCGCCGTTAGGAGGAGATAAGAACGTTACCGTTCCTGATGAACGACGTGAATTGACATGGAATGTCCCCACTATGTCTCATCTTGATCCCCGAACCGCACAATGCGATAATGAAGTGCGAAGAATTCTAGATCTACAGAGTGTAGCCCAAAATATGCCAGACGCTTTCTCTGATCTAGCTAAAGTGACGAGATCACATATACCTGCTGCAAATGTGCCTGCAAGAATAGATGTCCCTGTAGGACGCATTGTCCCGGATGGACGAGGTACGACAATGGCGGCTAACCAATCACATGTCTCTGCCCACAAGCGAGGTAGACCACTAGGTTCGAAGGATTCCTATCCCCGGAAGAGGGTAAAACCAGCACAAACGAATCCACTAGACATCGCGATCTCAACTGATCCATCTCACGAGATAATTCCAGATTATGGGTCCGTCCTAGAAGAGACAACGTTGGGGGACGCTCCAACGTTTGAACCCACTCTCGAGAATAGAGAAATCTCGATAAATTATGCATGCTTAAGTGAGATTTGGAATCGAAATGAGATTATCATCGATGATATATTTGTATTCGCAGTAGCTACTGAAATTATAACTAGCGATGATGTCGAACCTCGCTCCGTTGATGAATGTCAACGTAGAGACGATTGGCCAAAATGGAAAGAAGCAATTCAGGTCGAATTAGATTCCTTGACAAAAAGAAAAGTGTTCGGGCCTGTCGTTCCCACACCTCGACATGTTAAACCTGTAGGGTTTAAATGGGTATTTGTAAGGAAGCGTAATGAGGGAAACGAGATTGTAAGATACAAAGCTCGTCTAGTGGCGCAAGGATTCTCTCAACGCCCTGGGATTGATTACGATGAGACATATTCTCCTGTAATGGATGTTATAACATTCCGCTACCTCATCAGTTTGGCAATTTCTGAAAAACTGAATATACAGCTAATGGATGTGGTCACAGCTTATCTCTATGGGGATCTTGATACAGAGATATATATGAAAGTTCCTGAAGGACTTAAGATACCCGATACAAATAGTTCTAGACCACGGAACACCCTCTCCATTCGTTTGAGGCGTTCACTTTATGGATTGAAACAATCTGGACGGATGTGGTACAACCGTCTAAGTGAATATTTGATTGGGATGGGATATGTAAACAATGAACTATGCCCATGCGTGTTTATTAAGAAAACAAGTTCTGGATTTGTAATTGTGGCAGTTTATGTCGATGACATGAATCTGATTGGTACTCCTGAAGAGATCAAAGAAACCGCCAAGCACCTGAAGTCTGAATTCGAAATGAAAGATCTTGGGAGAACGAATTACTGCCTCGGCCTGGAGCTTGAGCACCGTGCTGATGGGATTCTGCTTCATCAATCAAACTACATCCAGAAGATGTTAAGACGCTTTAATGAGGATAAAGCGAAACCATCAAGCACACCCATGGTCATCCGAAGTCTAGATCCTAAGAAAGATCCGTTTCGTCCCGCAGATAATAACGAAGAGATATTGGCACCAGAAGTCCCATATCTAAGTGCAATAGGCGCATTATTGTACTTGACTCAATGCACTAGACCAGACATTTCATTTTCTGTGAACTTGTTAGCTAGATATAGCTCTGCACCAACACGCCGCCACTGGAATGGTATAAAAGATATTTTTCGTTATCTTAGAGGTACGATTGATATGGGCTTATTTTATCCCTATGCATCAAGAAATGGATCAAACCCCCTTGATCCTCAGAATGATGCTCGCCTTGTTGGATATGCTGATGCAGGCTATCTATCAGACCCACACAAGGCACGTTCCCAAACTGGTTATGTTTTTACCATTGGGAATACGGCAATTTCTTGGAGGTCTACAAAACAGACCCTTGTTGCTACCTCTTCGAATCATGCTGAGATTCTAGCTCTTCACGAAGCAGTACGTGAATGTATATGGTTGAGAGCCATCACAAAGCATGTTCAAAGCACATGTGGACTGCATTCCACCACTGATGAACCAACCACTATCCACGAGGATAATGCAGCTTGCATCGAGCAAATGAAGACAGGTTTCATCAAAGGAGACAACACCAAACATATTGCACCAAAGTTTTTCTTCAATCAGCAACAACAAGAGCATCAGAAGATTGAGGTCAAGCAGATTCGATCTGAAGACAATCGTGCAGACCTCTTCACTAAGTCACTACCAAAATCTACATTCCAGAAGCATGTACAAGGTATTGGTCTACGTAAATTATCTGAATTACCTAACATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 rve

Protein Analysis

797

Amino Acids

90.41

Weight (kDa)

7.6

Isoelectric Point (pI)

50.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_2 PF07727 284 - 527 3.4e-62 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 725, 1022
Acc36I ACCTGC 1 cut(s) 343
AccB1I GGYRCC 1 cut(s) 1645
AccB7I CCANNNNNTGG 1 cut(s) 560
AccI GTMKAC 3 cut(s) 448, 1991, 2362
AccII CGCG 1 cut(s) 521
AciI CCGC 9 cut(s) 93, 104, 134, 228, 410, 1030, 1404, 1620, 1765
AclI AACGTT 3 cut(s) 156, 584, 602
AclWI GGATC 7 cut(s) 212, 527, 1116, 1589, 1601, 1861, 1862
AcoI YGGCCR 1 cut(s) 782
AcsI RAATTY 2 cut(s) 255, 1424
AcuI CTGAAG 4 cut(s) 1161, 1407, 1436, 2307
AfaI GTAC 7 cut(s) 400, 1247, 1381, 1688, 1810, 2058, 2349
AfiI CCNNNNNNNGG 9 cut(s) 77, 140, 143, 477, 483, 484, 560, 1468, 1930
AflII CTTAAG 2 cut(s) 652, 1148
AflIII ACRYGT 4 cut(s) 424, 868, 2105, 2388
AgsI TTSAA 4 cut(s) 608, 1225, 2099, 2236
AhdI GACNNNNNGTC 1 cut(s) 380
AjiI CACGTC 1 cut(s) 179
AjnI CCWGG 2 cut(s) 976, 1467
AjuI GAANNNNNNNTTGG 2 cut(s) 1254, 1286
AloI GAACNNNNNNTCC 2 cut(s) 1175, 1207
Alw21I GWGCWC 1 cut(s) 1482
Alw26I GTCTC 7 cut(s) 116, 213, 433, 572, 768, 989, 2193
AlwI GGATC 7 cut(s) 212, 527, 1116, 1589, 1601, 1861, 1862
Ama87I CYCGRG 1 cut(s) 617
AoxI GGCC 3 cut(s) 782, 845, 1465
ApeKI GCWGC 2 cut(s) 338, 2159
ApoI RAATTY 2 cut(s) 255, 1424
Asp700I GAANNNNTTC 1 cut(s) 801
AspLEI GCGC 2 cut(s) 958, 1679
AspS9I GGNCC 3 cut(s) 5, 564, 845
AsuC2I CCSGG 2 cut(s) 386, 478
AsuII TTCGAA 3 cut(s) 461, 1428, 2021
AvaI CYCGRG 1 cut(s) 617
AvaII GGWCC 2 cut(s) 5, 564
BaeI ACNNNNGTAYC 2 cut(s) 1237, 1270
BalI TGGCCA 1 cut(s) 784
BanI GGYRCC 1 cut(s) 1645
BanII GRGCYC 1 cut(s) 72
BauI CACGAG 2 cut(s) 542, 2147
BbsI GAAGAC 3 cut(s) 121, 2186, 2295
Bbv12I GWGCWC 1 cut(s) 1482
BbvI GCAGC 2 cut(s) 325, 2171
BccI CCATC 8 cut(s) 13, 55, 383, 544, 1270, 1484, 1570, 2090
BceAI ACGGC 2 cut(s) 121, 1989
BcgI CGANNNNNNTGC 4 cut(s) 13, 47, 2138, 2172
BciT130I CCWGG 2 cut(s) 978, 1469
BcnI CCSGG 2 cut(s) 386, 478
BcoDI GTCTC 7 cut(s) 116, 213, 433, 572, 768, 989, 2193
BfmI CTRYAG 3 cut(s) 266, 369, 879
BfrI CTTAAG 2 cut(s) 652, 1148
BfuAI ACCTGC 1 cut(s) 343
BglII AGATCT 2 cut(s) 262, 1438
BisI GCNGC 4 cut(s) 339, 411, 1765, 2160
BlsI GCNGC 4 cut(s) 340, 412, 1766, 2161
Bme1390I CCNGG 4 cut(s) 386, 478, 978, 1469
Bme18I GGWCC 2 cut(s) 5, 564
BmeRI GACNNNNNGTC 1 cut(s) 380
BmeT110I CYCGRG 1 cut(s) 617
BmgBI CACGTC 1 cut(s) 179
BmgT120I GGNCC 3 cut(s) 5, 564, 845
BmiI GGNNCC 3 cut(s) 6, 565, 1647
BmrFI CCNGG 4 cut(s) 386, 478, 978, 1469
BmsI GCATC 5 cut(s) 1850, 1870, 1894, 2175, 2263
BpiI GAAGAC 3 cut(s) 121, 2186, 2295
BpmI CTGGAG 1 cut(s) 1490
Bpu14I TTCGAA 3 cut(s) 461, 1428, 2021
BpuEI CTTGAG 1 cut(s) 1496
BpuMI CCSGG 2 cut(s) 386, 478
Bsa29I ATCGAT 1 cut(s) 687
BsaAI YACGTR 2 cut(s) 2060, 2366
BsaBI GATNNNNATC 2 cut(s) 471, 683
BsaJI CCNNGG 6 cut(s) 476, 976, 977, 1178, 1462, 1575
BsaXI ACNNNNNCTCC 4 cut(s) 988, 1018, 1175, 1205
Bsc4I CCNNNNNNNGG 9 cut(s) 77, 140, 143, 477, 483, 484, 560, 1468, 1930
Bse1I ACTGG 2 cut(s) 1775, 1951
Bse8I GATNNNNATC 2 cut(s) 471, 683
BseBI CCWGG 2 cut(s) 978, 1469
BseCI ATCGAT 1 cut(s) 687
BseDI CCNNGG 6 cut(s) 476, 976, 977, 1178, 1462, 1575
BseGI GGATG 9 cut(s) 24, 47, 394, 1021, 1087, 1245, 1281, 1518, 1581
BseJI GATNNNNATC 2 cut(s) 471, 683
BseLI CCNNNNNNNGG 9 cut(s) 77, 140, 143, 477, 483, 484, 560, 1468, 1930
BseMII CTCAG 2 cut(s) 1886, 2022
BseNI ACTGG 2 cut(s) 1775, 1951
BseRI GAGGAG 1 cut(s) 159
BseXI GCAGC 2 cut(s) 325, 2171
BsgI GTGCAG 2 cut(s) 1737, 2319
Bsh1236I CGCG 1 cut(s) 521
BshFI GGCC 3 cut(s) 784, 847, 1467
BshNI GGYRCC 1 cut(s) 1645
BshVI ATCGAT 1 cut(s) 687
BsiHKAI GWGCWC 1 cut(s) 1482
BsiHKCI CYCGRG 1 cut(s) 617
BsiSI CCGG 2 cut(s) 386, 478
BslFI GGGAC 6 cut(s) 182, 350, 368, 605, 1602, 1640
BslI CCNNNNNNNGG 9 cut(s) 77, 140, 143, 477, 483, 484, 560, 1468, 1930
BsmAI GTCTC 7 cut(s) 116, 213, 433, 572, 768, 989, 2193
BsmBI CGTCTC 1 cut(s) 768
BsmFI GGGAC 6 cut(s) 182, 350, 368, 605, 1602, 1640
BsmI GAATGC 1 cut(s) 2116
BsnI GGCC 3 cut(s) 784, 847, 1467
BsoBI CYCGRG 1 cut(s) 617
Bsp119I TTCGAA 3 cut(s) 461, 1428, 2021
Bsp1286I GDGCHC 2 cut(s) 72, 1482
Bsp1407I TGTACA 1 cut(s) 2347
Bsp19I CCATGG 1 cut(s) 1575
Bsp68I TCGCGA 1 cut(s) 521
BspACI CCGC 9 cut(s) 93, 104, 134, 228, 410, 1030, 1404, 1620, 1765
BspANI GGCC 3 cut(s) 784, 847, 1467
BspCNI CTCAG 2 cut(s) 1885, 2023
BspDI ATCGAT 1 cut(s) 687
BspFNI CGCG 1 cut(s) 521
BspLI GGNNCC 3 cut(s) 6, 565, 1647
BspMI ACCTGC 1 cut(s) 343
BspPI GGATC 7 cut(s) 212, 527, 1116, 1589, 1601, 1861, 1862
BspQI GCTCTTC 1 cut(s) 2049
BspT104I TTCGAA 3 cut(s) 461, 1428, 2021
BspT107I GGYRCC 1 cut(s) 1645
BspTI CTTAAG 2 cut(s) 652, 1148
BsrGI TGTACA 1 cut(s) 2347
BsrI ACTGG 2 cut(s) 1775, 1951
BssECI CCNNGG 6 cut(s) 476, 976, 977, 1178, 1462, 1575
BssSI CACGAG 2 cut(s) 542, 2147
BssT1I CCWWGG 1 cut(s) 1575
Bst2BI CACGAG 2 cut(s) 542, 2147
Bst2UI CCWGG 2 cut(s) 978, 1469
Bst4CI ACNGT 4 cut(s) 127, 163, 1253, 1484
Bst6I CTCTTC 7 cut(s) 476, 570, 1383, 1629, 2023, 2049, 2312
BstAFI CTTAAG 2 cut(s) 652, 1148
BstAUI TGTACA 1 cut(s) 2347
BstBAI YACGTR 2 cut(s) 2060, 2366
BstBI TTCGAA 3 cut(s) 461, 1428, 2021
BstC8I GCNNGC 5 cut(s) 351, 649, 1885, 1909, 2164
BstDEI CTNAG 7 cut(s) 1256, 1599, 1664, 1802, 1872, 2031, 2313
BstDSI CCRYGG 2 cut(s) 1178, 1575
BstF5I GGATG 9 cut(s) 24, 47, 394, 1021, 1087, 1245, 1281, 1518, 1581
BstFNI CGCG 1 cut(s) 521
BstHHI GCGC 2 cut(s) 958, 1679
BstMAI GTCTC 7 cut(s) 116, 213, 433, 572, 768, 989, 2193
BstMWI GCNNNNNNNGC 2 cut(s) 101, 1676
BstNI CCWGG 2 cut(s) 978, 1469
BstNSI RCATGY 7 cut(s) 428, 651, 872, 2096, 2109, 2348, 2392
BstSCI CCNGG 4 cut(s) 384, 476, 976, 1467
BstSFI CTRYAG 3 cut(s) 266, 369, 879
BstSNI TACGTA 1 cut(s) 2366
BstUI CGCG 1 cut(s) 521
BstV1I GCAGC 2 cut(s) 325, 2171
BstV2I GAAGAC 3 cut(s) 121, 2186, 2295
BstX2I RGATCY 5 cut(s) 262, 1108, 1438, 1594, 1606
BstXI CCANNNNNNTGG 1 cut(s) 1775
BstYI RGATCY 5 cut(s) 262, 1108, 1438, 1594, 1606
Bsu15I ATCGAT 1 cut(s) 687
BsuRI GGCC 3 cut(s) 784, 847, 1467
BsuTUI ATCGAT 1 cut(s) 687
BtgI CCRYGG 2 cut(s) 1178, 1575
BtgZI GCGATG 2 cut(s) 502, 747
BtrI CACGTC 1 cut(s) 179
BtsCI GGATG 9 cut(s) 24, 47, 394, 1021, 1087, 1245, 1281, 1518, 1581
BtsIMutI CAGTG 2 cut(s) 1768, 2124
BtuMI TCGCGA 1 cut(s) 521
BveI ACCTGC 1 cut(s) 343
Cac8I GCNNGC 5 cut(s) 351, 649, 1885, 1909, 2164
CfoI GCGC 2 cut(s) 958, 1679
Cfr13I GGNCC 3 cut(s) 5, 564, 845
ClaI ATCGAT 1 cut(s) 687
CseI GACGC 4 cut(s) 302, 384, 602, 1545
Csp6I GTAC 7 cut(s) 399, 1246, 1380, 1687, 1809, 2057, 2348
CviQI GTAC 7 cut(s) 399, 1246, 1380, 1687, 1809, 2057, 2348
DdeI CTNAG 7 cut(s) 1256, 1599, 1664, 1802, 1872, 2031, 2313
DraI TTTAAA 1 cut(s) 889
DriI GACNNNNNGTC 1 cut(s) 380
EaeI YGGCCR 1 cut(s) 782
Eam1104I CTCTTC 7 cut(s) 476, 570, 1383, 1629, 2023, 2049, 2312
Eam1105I GACNNNNNGTC 1 cut(s) 380
EarI CTCTTC 7 cut(s) 476, 570, 1383, 1629, 2023, 2049, 2312
Eco105I TACGTA 1 cut(s) 2366
Eco130I CCWWGG 1 cut(s) 1575
Eco24I GRGCYC 1 cut(s) 72
Eco47I GGWCC 2 cut(s) 5, 564
Eco57I CTGAAG 4 cut(s) 1161, 1407, 1436, 2307
Eco88I CYCGRG 1 cut(s) 617
EcoO109I RGGNCCY 1 cut(s) 5
EcoRI GAATTC 2 cut(s) 255, 1424
EcoRII CCWGG 2 cut(s) 976, 1467
EcoT14I CCWWGG 1 cut(s) 1575
EcoT22I ATGCAT 2 cut(s) 649, 1843
EcoT38I GRGCYC 1 cut(s) 72
ErhI CCWWGG 1 cut(s) 1575
Esp3I CGTCTC 1 cut(s) 768
FalI AAGNNNNNCTT 2 cut(s) 2216, 2248
FaqI GGGAC 6 cut(s) 182, 350, 368, 605, 1602, 1640
FauI CCCGC 2 cut(s) 141, 1627
FblI GTMKAC 3 cut(s) 448, 1991, 2362
Fnu4HI GCNGC 4 cut(s) 339, 411, 1765, 2160
FokI GGATG 9 cut(s) 31, 34, 401, 1028, 1094, 1252, 1288, 1505, 1568
FriOI GRGCYC 1 cut(s) 72
Fsp4HI GCNGC 4 cut(s) 339, 411, 1765, 2160
GlaI GCGC 2 cut(s) 957, 1678
GluI GCNGC 4 cut(s) 339, 411, 1765, 2160
GsuI CTGGAG 1 cut(s) 1490
HaeIII GGCC 3 cut(s) 784, 847, 1467
HapII CCGG 2 cut(s) 386, 478
HgaI GACGC 4 cut(s) 302, 384, 602, 1545
HhaI GCGC 2 cut(s) 958, 1679
Hin6I GCGC 2 cut(s) 956, 1677
HinP1I GCGC 2 cut(s) 956, 1677
HincII GTYRAC 1 cut(s) 767
HindII GTYRAC 1 cut(s) 767
HpaII CCGG 2 cut(s) 386, 478
Hpy166II GTNNAC 8 cut(s) 449, 767, 1211, 1288, 1729, 1992, 2111, 2363
Hpy8I GTNNAC 8 cut(s) 449, 767, 1211, 1288, 1729, 1992, 2111, 2363
Hpy99I CGWCG 1 cut(s) 180
HpyAV CCTTC 3 cut(s) 9, 457, 1136
HpyCH4III ACNGT 4 cut(s) 127, 163, 1253, 1484
HpyCH4IV ACGT 8 cut(s) 156, 178, 584, 602, 769, 1936, 2059, 2365
HpyF10VI GCNNNNNNNGC 2 cut(s) 101, 1676
HpyF3I CTNAG 7 cut(s) 1256, 1599, 1664, 1802, 1872, 2031, 2313
HpySE526I ACGT 8 cut(s) 156, 178, 584, 602, 769, 1936, 2059, 2365
HspAI GCGC 2 cut(s) 956, 1677
LguI GCTCTTC 1 cut(s) 2049
LmnI GCTCC 3 cut(s) 601, 755, 1471
Lsp1109I GCAGC 2 cut(s) 325, 2171
LweI GCATC 5 cut(s) 1850, 1870, 1894, 2175, 2263
MaeII ACGT 8 cut(s) 156, 178, 584, 602, 769, 1936, 2059, 2365
MaeIII GTNAC 5 cut(s) 110, 157, 316, 1087, 2316
MflI RGATCY 5 cut(s) 262, 1108, 1438, 1594, 1606
MhlI GDGCHC 2 cut(s) 72, 1482
MlsI TGGCCA 1 cut(s) 784
MluNI TGGCCA 1 cut(s) 784
MlyI GAGTC 1 cut(s) 1687
MmeI TCCRAC 2 cut(s) 623, 1873
Mox20I TGGCCA 1 cut(s) 784
Mph1103I ATGCAT 2 cut(s) 649, 1843
MroXI GAANNNNTTC 1 cut(s) 801
MscI TGGCCA 1 cut(s) 784
MseI TTAA 7 cut(s) 653, 873, 888, 1149, 1317, 1532, 1542
MslI CAYNNNNRTG 3 cut(s) 117, 1574, 1773
Msp20I TGGCCA 1 cut(s) 784
MspCI CTTAAG 2 cut(s) 652, 1148
MspI CCGG 2 cut(s) 386, 478
MspR9I CCNGG 4 cut(s) 386, 478, 978, 1469
Mva1269I GAATGC 1 cut(s) 2116
MvaI CCWGG 2 cut(s) 978, 1469
MvnI CGCG 1 cut(s) 521
MwoI GCNNNNNNNGC 2 cut(s) 101, 1676
NciI CCSGG 2 cut(s) 386, 478
NcoI CCATGG 1 cut(s) 1575
NlaIV GGNNCC 3 cut(s) 6, 565, 1647
NmeAIII GCCGAG 1 cut(s) 1443
NmuCI GTSAC 4 cut(s) 110, 316, 1087, 2316
NruI TCGCGA 1 cut(s) 521
NsiI ATGCAT 2 cut(s) 649, 1843
NspI RCATGY 7 cut(s) 428, 651, 872, 2096, 2109, 2348, 2392
NspV TTCGAA 3 cut(s) 461, 1428, 2021
PaeI GCATGC 1 cut(s) 651
PaeR7I CTCGAG 1 cut(s) 617
PasI CCCWGGG 1 cut(s) 977
PciI ACATGT 4 cut(s) 424, 868, 2105, 2388
PciSI GCTCTTC 1 cut(s) 2049
PcsI WCGNNNNNNNCGW 2 cut(s) 849, 1204
PctI GAATGC 1 cut(s) 2116
PdmI GAANNNNTTC 1 cut(s) 801
PflMI CCANNNNNTGG 1 cut(s) 560
PfoI TCCNGGA 1 cut(s) 384
PkrI GCNGC 4 cut(s) 340, 412, 1766, 2161
PleI GAGTC 1 cut(s) 1687
PpsI GAGTC 1 cut(s) 1687
Ppu21I YACGTR 2 cut(s) 2060, 2366
PpuMI RGGWCCY 1 cut(s) 5
PscI ACATGT 4 cut(s) 424, 868, 2105, 2388
PsiI TTATAA 2 cut(s) 725, 1022
Psp1406I AACGTT 3 cut(s) 156, 584, 602
Psp5II RGGWCCY 1 cut(s) 5
Psp6I CCWGG 2 cut(s) 976, 1467
PspGI CCWGG 2 cut(s) 976, 1467
PspN4I GGNNCC 3 cut(s) 6, 565, 1647
PspPI GGNCC 3 cut(s) 5, 564, 845
PspPPI RGGWCCY 1 cut(s) 5
PsuI RGATCY 5 cut(s) 262, 1108, 1438, 1594, 1606
RruI TCGCGA 1 cut(s) 521
RsaI GTAC 7 cut(s) 400, 1247, 1381, 1688, 1810, 2058, 2349
RsaNI GTAC 7 cut(s) 399, 1246, 1380, 1687, 1809, 2057, 2348
RseI CAYNNNNRTG 3 cut(s) 117, 1574, 1773
SapI GCTCTTC 1 cut(s) 2049
SaqAI TTAA 7 cut(s) 653, 873, 888, 1149, 1317, 1532, 1542
SatI GCNGC 4 cut(s) 339, 411, 1765, 2160
Sau96I GGNCC 3 cut(s) 5, 564, 845
SchI GAGTC 1 cut(s) 1687
ScrFI CCNGG 4 cut(s) 386, 478, 978, 1469
SduI GDGCHC 2 cut(s) 72, 1482
SfaNI GCATC 5 cut(s) 1850, 1870, 1894, 2175, 2263
SfcI CTRYAG 3 cut(s) 266, 369, 879
Sfr274I CTCGAG 1 cut(s) 617
SfuI TTCGAA 3 cut(s) 461, 1428, 2021
SinI GGWCC 2 cut(s) 5, 564
SlaI CTCGAG 1 cut(s) 617
SmiMI CAYNNNNRTG 3 cut(s) 117, 1574, 1773
SmlI CTYRAG 4 cut(s) 617, 652, 1148, 1475
SmoI CTYRAG 4 cut(s) 617, 652, 1148, 1475
SnaBI TACGTA 1 cut(s) 2366
SphI GCATGC 1 cut(s) 651
SsiI CCGC 9 cut(s) 93, 104, 134, 228, 410, 1030, 1404, 1620, 1765
SspI AATATT 1 cut(s) 1265
StyD4I CCNGG 4 cut(s) 384, 476, 976, 1467
StyI CCWWGG 1 cut(s) 1575
TaaI ACNGT 4 cut(s) 127, 163, 1253, 1484
TaiI ACGT 8 cut(s) 159, 181, 587, 605, 772, 1939, 2062, 2368
TatI WGTACW 2 cut(s) 1686, 2347
TauI GCSGC 2 cut(s) 413, 1767
Tru1I TTAA 7 cut(s) 653, 873, 888, 1149, 1317, 1532, 1542
Tru9I TTAA 7 cut(s) 653, 873, 888, 1149, 1317, 1532, 1542
TscAI CASTG 2 cut(s) 1775, 2131
TseFI GTSAC 4 cut(s) 110, 316, 1087, 2316
TseI GCWGC 2 cut(s) 338, 2159
Tsp45I GTSAC 4 cut(s) 110, 316, 1087, 2316
TspGWI ACGGA 5 cut(s) 556, 742, 1195, 1252, 1599
TspRI CASTG 2 cut(s) 1775, 2131
Van91I CCANNNNNTGG 1 cut(s) 560
Vha464I CTTAAG 2 cut(s) 652, 1148
VpaK11BI GGWCC 2 cut(s) 5, 564
XapI RAATTY 2 cut(s) 255, 1424
XbaI TCTAGA 3 cut(s) 259, 1172, 1591
XceI RCATGY 7 cut(s) 428, 651, 872, 2096, 2109, 2348, 2392
XhoI CTCGAG 1 cut(s) 617
XmiI GTMKAC 3 cut(s) 448, 1991, 2362
XmnI GAANNNNTTC 1 cut(s) 801
Zsp2I ATGCAT 2 cut(s) 649, 1843
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.