Rw7G041220

aspartic-type endopeptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0004874
Physical Location & Seq
Reverse (-)
64221120 .. 64224842
3723 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G041220.1

Sequence Viewer

Length: 3723 bp
ATGTCTCCGGGAGAATTGGAATGCCTTCTAGATAGTGGCACCACACATACTATATTACGACATAGGCAATTATTCTTATGGATGACGCCTAGTCAATCTTCAGTGACCACGATGGCAGGACCATCTAAATTGATTCATGGTCGTGGACCAGCTCAATTTCTATTGCCGAATGGCACAAATATTAATGTCGCCGAAGCTTTATATGCTCCAAGGGCTGGAAGAACCCTATTAAGTTTTAAAGATATAAGAGCCAATGATCTTCATGTGAAAACACATTGTGAGAATGGACAAGAGTTCCTTTACATCACCTCTAATAACTACGGAAATACACGAGTATTAGAGAAACTCATGTGTCGCTCTAGTGGATTGTACGCAACCACTATTAGAGTCATTGAATCCAACCATGTCATGAATGAAAATTTATGGGATTCCGACACATACAGGCTTTGGCACGACCGTTTGGGACACCCAGGTCGTGATATGATGATCCGTATATTAAAAACTTCACACGGACATCCATTCTTTAAAACAAAGAGGAATACGAACCAAAAAGAGGTTCAAAGAATTACTTCTAAAGCATATCATTCGTTTTGCAAAGCTTGCTCTTTAGCAAAAGTAGGATCGAGACCATCCTATGCAAAAGACATTAAAGAAAATATACCATTCTTGCAAAGAATACAAGGTGATATCTGTGGACCTATCCATCCAACTTGCGGACCATTTAGATATTTTATGGTGTTGGTTGATGCATCGACACGTTGGTCACATGTCGTGCTCTTATCCACAAGAAATGCTGCATTTGCTAAACTCCTAGCACAAATCATTAAGTTAAGGGCTCACCACCCTGATCATCCCATTAAGTCAATTCGTCTTGACAATGCTGGGGAGTTTACATCAAAAACTTTTGATGATTATTGCATGTCCATTGGGATCGAGGTTGAACACCCCGTCCCTCATGTACATACCCAAAATGGTCTCGCAGAAGCTGCCATTAAAAGACTTCAAATGGTTGCTAGGGCATTGGTTATGCGCACCAATCTCCCTATTTCTGCTTGGGGCTATGCAATATTACATGCAGCTGTGCTTATTCGTCTAAGGCCCACTGCCACCCAACCTTTTTCTGCATCCCAGATGGTGACTGGGTATGAGCCTAATGTCTCACATTTACGCATATTTGGGTGTGCAGTCTATGTGCCAATTGCGCCACCACAGCGCACTAAAATGGGTCCTCAAAGACGATTAGGCCTTTATGTTGGCTATGACTCTCCAACCATAATCCGCTACATAGAACCCTTGACAGGTGATCTATTTACCGCTAGATTTGCGGATTGTCACTTTGATGAGACAGTCTTCCCGTCTTTAGGGGGAGACATGAACATAACTAATGTTCAACAAAAAGGACAAGAATTGTCGTGGTTTGTCCCCACTTTGTCTCATCTTGATCCCCGAACTGCACAATCTGAAAAAGAAGTGCAGAGAATTCTCGATCTTCAGAACGTAGCAGAATCGATGCCTGATGCGTTTTCTGATATCGCTAAAGTGACAAGATCACACATACCTGCTGCAAATACACCTGCAAGGATTGATGTCCCTAAAAGACATAATGCCGGCTCAAAAATACATGAGAATGGCGCCAACGTTCCCTCTATGGGTGACACATTGGCTAGGCCCACGGCTCCTGCCAGGAAGCGCGGGAGACCTATAGGTTCGATGGATTCTCGCCCAAGAAAGAAGGCGAGTTTGGCACAAAATAATAATCCATTAATCATAGATATGAATAATCCATCCCACGAGAATATTCCGGATTATGGTTATGTCCAAGAGACACCATTGGGGGACGCTCCAATGTCAGAACCAACTCCAGAGAATAGAGAAATCTCCATAAATTACAATAATGTATATGAGATGATGGATAGAAATTCTATGGCAATTGATGATGCGTTTGCATATCATGTTGCAAAAGGAATCATAGAATATGATGATATCGAACCTCGCTCTGTTGAAGAATGTCAACGAAGAGCGGATTGGCCTAAATGGAAAAATGCGATCCAGACTGAATTGGATTCACTAACAAAGAGACAGGTATTTGGGCCTATAATGCTGACACCACCAAATATAAAGCCTGTTGGCCATAAATGGGTCTTTGTTAGAAAACGTAATGAGAAAAATGAGGTGATGAGATATAAAGCCCGCCTCGTGGCGCAAGGTTTCTCACAACGCCCTGGAATCGACTACGAGGAGACATACTCTCCCGTAATGGACATTATAACGTTCCGCTACCTTGTCAGTTTGGTAGTTTCCGAAAAACTTGACATGCAGCTTATGGATGTAATTACAGCATATCTCTATGGGGATCTAGATTCAGAGATATATATGAAGGTTCCTGATGGACTTCAATTACCAAAATCAAGTGGCTCTAAACCACGGAGCGCGTTTTCAATAAAATTAAGACGCTCACTATATGGATTGAAACAATCCGGACGGATGTGGTATAACCGTCTAAGTGACTACTTGATTGGAAAGGGATATATTAACAATGAAATATGCCCTTGCGTGTTCATTAAAAGAACAAGTTCCGGATTTGCAATTGTAGCAGTTTATGTCGATGACATGAACCTAATTGGAACTCTAGATGAGTTAAAGGAAACTGCTAAATATTTGGAATCCGAGTTTGAGATGAAAGATCTTGGGAAAACACGGTTTTGCCTCGGAATAGAACTCGAGCACCGTAGTGATGGGATTATGATCCATCAGTCAGCATATACTCAAAAATTACTAAGGCGCTTTAATGAAGATAAAGCAAAGCCTGTAAGTACTCCCATGATCGGCCGAAGTCTTGAGCCCACAAAAGATCCGTTTCGTCCAAGGGATGAGGACGAAGAATTATTAGAGGCTGAAGTACCCTATCTAAGTGCGATAGGCGCATTATTGTATTTAGCACAATGCACAAGACCAGATATCTCATTCGCAGTGAACTTGTTAGCTCGACATAGTTCTGCGCCAACACGCCGCCATTGGATTGGCATAAAGACAATCTTTCGATACTTAAAAGGTACGATTGATATGGGCTTGTTTTATCCCTACAGAGAGAAAATAAAAGACGGAAGATTGGGATCAGACCCTATGAGGCAAAAAGCCACCTTCCGTAATACAGACGCCGGCAACACCATCCATGGTGACCGACGTTCTCCTCCTCCCCTCCATCAAAATAAAAATAATGTTTTGATGGGCTTTGCTGATGCAGGGTATCTCTCTGACCCTCATAAAGGTCGCTCCCAAACAGGTTATGTCTTTACCATGGGAAGCACGGCGATATCTTGGAGATCTACAAAACAGACACTTGTCGCTACTTCCTCAAATCATGCAGAGATTATTGCTCTACATGAAGCTGTGCGTGAATGCATATGGCTAAGGTCTATAAATAGACACATTCGAGGAACTTGTGGTTTGAAGTTAACCACAGATAAACCTACATGCATTTACGAAGATAATGCAGCTTGTATTGAGCAAATGAAATTAGGTTTTATCAAGGGTGACAATACTAAACATATATCACCGAAATTCTTTTACAATCAGCAACAGCAAACACTTCTAAACATTGAAGTAAATCAAATCCGATCAGAGGATAATGTAGCGGACTTATTTACTAAGTCATTACCAAAAACCCCTTTCGAGAAACATGTGAAGAGTATCGGATTAAGAAGATTATCCGAACTCTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 rve

Protein Analysis

1240

Amino Acids

140.36

Weight (kDa)

9.22

Isoelectric Point (pI)

48.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pol_BBD PF22936 9 - 83 7.5e-06 Pol polyprotein, beta-barrel domain
gag_pre-integrs PF13976 122 - 205 2.8e-07 GAG-pre-integrase domain
rve PF00665 224 - 315 1.2e-09 Integrase core domain
RVT_2 PF07727 702 - 942 4.8e-58 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2267
AarI CACCTGC 1 cut(s) 1582
AasI GACNNNNNNGTC 2 cut(s) 471, 760
Acc16I TGCGCA 1 cut(s) 1031
Acc36I ACCTGC 2 cut(s) 1567, 1582
AccB1I GGYRCC 2 cut(s) 38, 1631
AccB7I CCANNNNNTGG 1 cut(s) 215
AccBSI CCGCTC 1 cut(s) 2021
AccII CGCG 2 cut(s) 1692, 2432
AccIII TCCGGA 3 cut(s) 1801, 2477, 2576
AclI AACGTT 2 cut(s) 1638, 2270
AclWI GGATC 9 cut(s) 481, 628, 938, 1436, 2041, 2361, 2740, 2846, 3121
AcoI YGGCCR 2 cut(s) 2128, 2827
AcsI RAATTY 4 cut(s) 418, 1479, 1918, 3560
AcuI CTGAAG 3 cut(s) 84, 1475, 2916
AcyI GRCGYC 3 cut(s) 86, 1632, 3156
AdeI CACNNNGTG 1 cut(s) 278
AfaI GTAC 5 cut(s) 371, 960, 2815, 2901, 3055
AflIII ACRYGT 3 cut(s) 755, 766, 3679
AhdI GACNNNNNGTC 1 cut(s) 90
AjnI CCWGG 3 cut(s) 469, 1682, 2221
AjuI GAANNNNNNNTTGG 6 cut(s) 540, 572, 1724, 1756, 2008, 2040
AleI CACNNNNGTG 1 cut(s) 2730
AloI GAACNNNNNNTCC 2 cut(s) 933, 965
AluBI AGCT 9 cut(s) 152, 197, 599, 986, 1079, 2320, 2984, 3389, 3497
AluI AGCT 9 cut(s) 152, 197, 599, 986, 1079, 2320, 2984, 3389, 3497
Alw21I GWGCWC 2 cut(s) 777, 2727
AlwI GGATC 9 cut(s) 481, 628, 938, 1436, 2041, 2361, 2740, 2846, 3121
AlwNI CAGNNNCTG 1 cut(s) 986
Ama87I CYCGRG 1 cut(s) 2720
Aor13HI TCCGGA 3 cut(s) 1801, 2477, 2576
AoxI GGCC 7 cut(s) 1097, 1243, 1667, 2027, 2090, 2128, 2827
ApeKI GCWGC 6 cut(s) 794, 986, 1076, 1562, 2317, 3494
ApoI RAATTY 4 cut(s) 418, 1479, 1918, 3560
AseI ATTAAT 2 cut(s) 183, 1763
Asp700I GAANNNNTTC 3 cut(s) 24, 568, 2572
AspS9I GGNCC 8 cut(s) 119, 146, 695, 716, 1098, 1226, 1668, 2090
AsuC2I CCSGG 1 cut(s) 9
AvaI CYCGRG 1 cut(s) 2720
AvaII GGWCC 5 cut(s) 119, 146, 695, 716, 1226
BalI TGGCCA 1 cut(s) 2130
BanI GGYRCC 2 cut(s) 38, 1631
BanII GRGCYC 2 cut(s) 838, 2844
BauI CACGAG 3 cut(s) 330, 1790, 2195
BbsI GAAGAC 1 cut(s) 1342
Bbv12I GWGCWC 2 cut(s) 777, 2727
BbvI GCAGC 6 cut(s) 781, 973, 1088, 1549, 2329, 3506
BceAI ACGGC 2 cut(s) 1689, 3324
BcgI CGANNNNNNTGC 4 cut(s) 567, 601, 3473, 3507
BciT130I CCWGG 3 cut(s) 471, 1684, 2223
BclI TGATCA 1 cut(s) 847
BcnI CCSGG 1 cut(s) 9
BfaI CTAG 9 cut(s) 29, 90, 360, 812, 1014, 1317, 1665, 2357, 2630
BfmI CTRYAG 2 cut(s) 1701, 3082
BfoI RGCGCY 2 cut(s) 1635, 2785
BfuAI ACCTGC 2 cut(s) 1567, 1582
BglII AGATCT 2 cut(s) 2683, 3323
BisI GCNGC 7 cut(s) 795, 987, 1077, 1563, 2318, 3010, 3495
BlsI GCNGC 7 cut(s) 796, 988, 1078, 1564, 2319, 3011, 3496
BmcAI AGTACT 1 cut(s) 2815
Bme1390I CCNGG 4 cut(s) 9, 471, 1684, 2223
Bme18I GGWCC 5 cut(s) 119, 146, 695, 716, 1226
BmeRI GACNNNNNGTC 1 cut(s) 90
BmeT110I CYCGRG 1 cut(s) 2720
BmgT120I GGNCC 8 cut(s) 119, 146, 695, 716, 1098, 1226, 1668, 2090
BmiI GGNNCC 5 cut(s) 40, 1227, 1633, 1677, 2382
BmrFI CCNGG 4 cut(s) 9, 471, 1684, 2223
BmrI ACTGGG 1 cut(s) 1149
BmsI GCATC 7 cut(s) 736, 758, 1133, 1500, 1507, 1927, 3229
BmuI ACTGGG 1 cut(s) 1149
BoxI GACNNNNGTC 1 cut(s) 3341
BpiI GAAGAC 1 cut(s) 1342
BplI GAGNNNNNCTC 2 cut(s) 2231, 2263
BpmI CTGGAG 1 cut(s) 1845
Bpu10I CCTNAGC 1 cut(s) 3410
BpuEI CTTGAG 1 cut(s) 2858
BpuMI CCSGG 1 cut(s) 9
Bsa29I ATCGAT 1 cut(s) 1508
BsaBI GATNNNNATC 2 cut(s) 2744, 3112
BsaHI GRCGYC 3 cut(s) 86, 1632, 3156
BsaI GGTCTC 3 cut(s) 619, 980, 1690
BsaJI CCNNGG 9 cut(s) 209, 469, 1671, 2221, 2423, 2707, 2864, 3172, 3297
BsaWI WCCGGW 3 cut(s) 1801, 2477, 2576
BsaXI ACNNNNNCTCC 2 cut(s) 2233, 2263
Bse118I RCCGGY 2 cut(s) 1607, 3158
Bse1I ACTGG 1 cut(s) 1144
Bse8I GATNNNNATC 2 cut(s) 2744, 3112
BseAI TCCGGA 3 cut(s) 1801, 2477, 2576
BseBI CCWGG 3 cut(s) 471, 1684, 2223
BseCI ATCGAT 1 cut(s) 1508
BseDI CCNNGG 9 cut(s) 209, 469, 1671, 2221, 2423, 2707, 2864, 3172, 3297
BseJI GATNNNNATC 2 cut(s) 2744, 3112
BseNI ACTGG 1 cut(s) 1144
BseRI GAGGAG 3 cut(s) 2252, 3180, 3183
BseX3I CGGCCG 1 cut(s) 2827
BseXI GCAGC 6 cut(s) 781, 973, 1088, 1549, 2329, 3506
BseYI CCCAGC 1 cut(s) 881
BsgI GTGCAG 3 cut(s) 1203, 1437, 1493
Bsh1236I CGCG 2 cut(s) 1692, 2432
Bsh1285I CGRYCG 2 cut(s) 457, 2830
BshFI GGCC 7 cut(s) 1099, 1245, 1669, 2029, 2092, 2130, 2829
BshNI GGYRCC 2 cut(s) 38, 1631
BshVI ATCGAT 1 cut(s) 1508
BsiEI CGRYCG 2 cut(s) 457, 2830
BsiHKAI GWGCWC 2 cut(s) 777, 2727
BsiHKCI CYCGRG 1 cut(s) 2720
BsiSI CCGG 6 cut(s) 8, 1608, 1802, 2478, 2577, 3159
BslFI GGGAC 5 cut(s) 477, 935, 1406, 1574, 1850
BsmFI GGGAC 5 cut(s) 477, 935, 1406, 1574, 1850
BsmI GAATGC 2 cut(s) 26, 3404
BsnI GGCC 7 cut(s) 1099, 1245, 1669, 2029, 2092, 2130, 2829
Bso31I GGTCTC 3 cut(s) 619, 980, 1690
BsoBI CYCGRG 1 cut(s) 2720
Bsp1286I GDGCHC 4 cut(s) 777, 838, 2727, 2844
Bsp13I TCCGGA 3 cut(s) 1801, 2477, 2576
Bsp1407I TGTACA 1 cut(s) 958
Bsp19I CCATGG 2 cut(s) 3172, 3297
BspANI GGCC 7 cut(s) 1099, 1245, 1669, 2029, 2092, 2130, 2829
BspDI ATCGAT 1 cut(s) 1508
BspEI TCCGGA 3 cut(s) 1801, 2477, 2576
BspFNI CGCG 2 cut(s) 1692, 2432
BspHI TCATGA 2 cut(s) 408, 3719
BspLI GGNNCC 5 cut(s) 40, 1227, 1633, 1677, 2382
BspMI ACCTGC 2 cut(s) 1567, 1582
BspPI GGATC 9 cut(s) 481, 628, 938, 1436, 2041, 2361, 2740, 2846, 3121
BspQI GCTCTTC 1 cut(s) 2011
BspT107I GGYRCC 2 cut(s) 38, 1631
BspTNI GGTCTC 3 cut(s) 619, 980, 1690
BsrBI CCGCTC 1 cut(s) 2021
BsrFI RCCGGY 2 cut(s) 1607, 3158
BsrGI TGTACA 1 cut(s) 958
BsrI ACTGG 1 cut(s) 1144
BssAI RCCGGY 2 cut(s) 1607, 3158
BssECI CCNNGG 9 cut(s) 209, 469, 1671, 2221, 2423, 2707, 2864, 3172, 3297
BssNI GRCGYC 3 cut(s) 86, 1632, 3156
BssSI CACGAG 3 cut(s) 330, 1790, 2195
BssT1I CCWWGG 4 cut(s) 209, 2864, 3172, 3297
Bst2BI CACGAG 3 cut(s) 330, 1790, 2195
Bst2UI CCWGG 3 cut(s) 471, 1684, 2223
Bst4CI ACNGT 5 cut(s) 458, 1348, 2498, 2700, 2729
Bst6I CTCTTC 2 cut(s) 2011, 3680
BstACI GRCGYC 3 cut(s) 86, 1632, 3156
BstAPI GCANNNNNTGC 2 cut(s) 600, 986
BstAUI TGTACA 1 cut(s) 958
BstC8I GCNNGC 4 cut(s) 601, 1609, 2191, 3160
BstDEI CTNAG 6 cut(s) 1094, 2501, 2777, 2909, 3410, 3648
BstDSI CCRYGG 4 cut(s) 1671, 2423, 3172, 3297
BstEII GGTNACC 1 cut(s) 3176
BstENI CCTNNNNNAGG 1 cut(s) 3264
BstFNI CGCG 2 cut(s) 1692, 2432
BstH2I RGCGCY 2 cut(s) 1635, 2785
BstMCI CGRYCG 2 cut(s) 457, 2830
BstNI CCWGG 3 cut(s) 471, 1684, 2223
BstNSI RCATGY 6 cut(s) 770, 922, 1076, 2317, 3477, 3683
BstPAI GACNNNNGTC 1 cut(s) 3341
BstPI GGTNACC 1 cut(s) 3176
BstSCI CCNGG 4 cut(s) 7, 469, 1682, 2221
BstSFI CTRYAG 2 cut(s) 1701, 3082
BstUI CGCG 2 cut(s) 1692, 2432
BstV1I GCAGC 6 cut(s) 781, 973, 1088, 1549, 2329, 3506
BstV2I GAAGAC 1 cut(s) 1342
BstX2I RGATCY 4 cut(s) 2353, 2683, 2851, 3323
BstXI CCANNNNNNTGG 1 cut(s) 3020
BstYI RGATCY 4 cut(s) 2353, 2683, 2851, 3323
BstZI CGGCCG 1 cut(s) 2827
Bsu15I ATCGAT 1 cut(s) 1508
BsuRI GGCC 7 cut(s) 1099, 1245, 1669, 2029, 2092, 2130, 2829
BsuTUI ATCGAT 1 cut(s) 1508
BtgI CCRYGG 4 cut(s) 1671, 2423, 3172, 3297
BtsI GCAGTG 2 cut(s) 1101, 2976
BtsIMutI CAGTG 3 cut(s) 108, 1101, 2976
BveI ACCTGC 2 cut(s) 1567, 1582
Cac8I GCNNGC 4 cut(s) 601, 1609, 2191, 3160
CaiI CAGNNNCTG 1 cut(s) 986
CciI TCATGA 2 cut(s) 408, 3719
Cfr10I RCCGGY 2 cut(s) 1607, 3158
Cfr13I GGNCC 8 cut(s) 119, 146, 695, 716, 1098, 1226, 1668, 2090
ClaI ATCGAT 1 cut(s) 1508
CseI GACGC 4 cut(s) 94, 1847, 2460, 3164
Csp6I GTAC 5 cut(s) 370, 959, 2814, 2900, 3054
CviQI GTAC 5 cut(s) 370, 959, 2814, 2900, 3054
DdeI CTNAG 6 cut(s) 1094, 2501, 2777, 2909, 3410, 3648
DinI GGCGCC 1 cut(s) 1633
DraI TTTAAA 2 cut(s) 238, 526
DraIII CACNNNGTG 1 cut(s) 278
DrdI GACNNNNNNGTC 2 cut(s) 471, 760
DriI GACNNNNNGTC 1 cut(s) 90
DseDI GACNNNNNNGTC 2 cut(s) 471, 760
EaeI YGGCCR 2 cut(s) 2128, 2827
EagI CGGCCG 1 cut(s) 2827
Eam1104I CTCTTC 2 cut(s) 2011, 3680
Eam1105I GACNNNNNGTC 1 cut(s) 90
EarI CTCTTC 2 cut(s) 2011, 3680
EclXI CGGCCG 1 cut(s) 2827
Eco130I CCWWGG 4 cut(s) 209, 2864, 3172, 3297
Eco147I AGGCCT 1 cut(s) 1245
Eco24I GRGCYC 2 cut(s) 838, 2844
Eco31I GGTCTC 3 cut(s) 619, 980, 1690
Eco32I GATATC 5 cut(s) 688, 1531, 1984, 2959, 3315
Eco47I GGWCC 5 cut(s) 119, 146, 695, 716, 1226
Eco52I CGGCCG 1 cut(s) 2827
Eco57I CTGAAG 3 cut(s) 84, 1475, 2916
Eco88I CYCGRG 1 cut(s) 2720
Eco91I GGTNACC 1 cut(s) 3176
EcoNI CCTNNNNNAGG 1 cut(s) 3264
EcoO109I RGGNCCY 1 cut(s) 1226
EcoO65I GGTNACC 1 cut(s) 3176
EcoRI GAATTC 1 cut(s) 1479
EcoRII CCWGG 3 cut(s) 469, 1682, 2221
EcoRV GATATC 5 cut(s) 688, 1531, 1984, 2959, 3315
EcoT14I CCWWGG 4 cut(s) 209, 2864, 3172, 3297
EcoT22I ATGCAT 3 cut(s) 751, 3404, 3479
EcoT38I GRGCYC 2 cut(s) 838, 2844
EgeI GGCGCC 1 cut(s) 1633
EheI GGCGCC 1 cut(s) 1633
ErhI CCWWGG 4 cut(s) 209, 2864, 3172, 3297
FalI AAGNNNNNCTT 6 cut(s) 282, 314, 553, 585, 3020, 3052
FaqI GGGAC 5 cut(s) 477, 935, 1406, 1574, 1850
FauI CCCGC 2 cut(s) 1685, 2198
FauNDI CATATG 1 cut(s) 3404
FbaI TGATCA 1 cut(s) 847
Fnu4HI GCNGC 7 cut(s) 795, 987, 1077, 1563, 2318, 3010, 3495
FriOI GRGCYC 2 cut(s) 838, 2844
Fsp4HI GCNGC 7 cut(s) 795, 987, 1077, 1563, 2318, 3010, 3495
FspAI RTGCGCAY 1 cut(s) 1031
FspBI CTAG 9 cut(s) 29, 90, 360, 812, 1014, 1317, 1665, 2357, 2630
FspI TGCGCA 1 cut(s) 1031
GluI GCNGC 7 cut(s) 795, 987, 1077, 1563, 2318, 3010, 3495
GsaI CCCAGC 1 cut(s) 885
GsuI CTGGAG 1 cut(s) 1845
HaeII RGCGCY 2 cut(s) 1635, 2785
HaeIII GGCC 7 cut(s) 1099, 1245, 1669, 2029, 2092, 2130, 2829
HapII CCGG 6 cut(s) 8, 1608, 1802, 2478, 2577, 3159
HgaI GACGC 4 cut(s) 94, 1847, 2460, 3164
Hin1I GRCGYC 3 cut(s) 86, 1632, 3156
HincII GTYRAC 2 cut(s) 2012, 3456
HindII GTYRAC 2 cut(s) 2012, 3456
HindIII AAGCTT 2 cut(s) 195, 597
HpaI GTTAAC 1 cut(s) 3456
HpaII CCGG 6 cut(s) 8, 1608, 1802, 2478, 2577, 3159
Hpy166II GTNNAC 6 cut(s) 146, 695, 891, 2012, 2974, 3456
Hpy8I GTNNAC 6 cut(s) 146, 695, 891, 2012, 2974, 3456
Hpy99I CGWCG 1 cut(s) 3186
HpyAV CCTTC 4 cut(s) 35, 1726, 2371, 3151
HpyCH4III ACNGT 5 cut(s) 458, 1348, 2498, 2700, 2729
HpyCH4IV ACGT 6 cut(s) 757, 1497, 1638, 2155, 2270, 3184
HpyF3I CTNAG 6 cut(s) 1094, 2501, 2777, 2909, 3410, 3648
HpySE526I ACGT 6 cut(s) 757, 1497, 1638, 2155, 2270, 3184
Hsp92I GRCGYC 3 cut(s) 86, 1632, 3156
KasI GGCGCC 1 cut(s) 1631
Kpn2I TCCGGA 3 cut(s) 1801, 2477, 2576
KroI GCCGGC 2 cut(s) 1607, 3158
KroNI GCCGGC 2 cut(s) 1609, 3160
Ksp22I TGATCA 1 cut(s) 847
KspAI GTTAAC 1 cut(s) 3456
LguI GCTCTTC 1 cut(s) 2011
LmnI GCTCC 5 cut(s) 211, 1681, 1846, 2427, 3278
Lsp1109I GCAGC 6 cut(s) 781, 973, 1088, 1549, 2329, 3506
LweI GCATC 7 cut(s) 736, 758, 1133, 1500, 1507, 1927, 3229
MaeI CTAG 9 cut(s) 29, 90, 360, 812, 1014, 1317, 1665, 2357, 2630
MaeII ACGT 6 cut(s) 757, 1497, 1638, 2155, 2270, 3184
MaeIII GTNAC 9 cut(s) 103, 762, 1135, 1331, 1540, 1652, 2504, 3176, 3533
MbiI CCGCTC 1 cut(s) 2021
MfeI CAATTG 3 cut(s) 1197, 1929, 2586
MflI RGATCY 4 cut(s) 2353, 2683, 2851, 3323
MhlI GDGCHC 4 cut(s) 777, 838, 2727, 2844
MlsI TGGCCA 1 cut(s) 2130
MluNI TGGCCA 1 cut(s) 2130
Mly113I GGCGCC 1 cut(s) 1632
MlyI GAGTC 2 cut(s) 396, 1256
MmeI TCCRAC 4 cut(s) 423, 456, 731, 1292
Mox20I TGGCCA 1 cut(s) 2130
Mph1103I ATGCAT 3 cut(s) 751, 3404, 3479
MroI TCCGGA 3 cut(s) 1801, 2477, 2576
MroNI GCCGGC 2 cut(s) 1607, 3158
MroXI GAANNNNTTC 3 cut(s) 24, 568, 2572
MscI TGGCCA 1 cut(s) 2130
MslI CAYNNNNRTG 6 cut(s) 141, 1220, 1338, 1626, 1772, 2730
Msp20I TGGCCA 1 cut(s) 2130
MspA1I CMGCKG 1 cut(s) 1079
MspI CCGG 6 cut(s) 8, 1608, 1802, 2478, 2577, 3159
MspR9I CCNGG 4 cut(s) 9, 471, 1684, 2223
MunI CAATTG 3 cut(s) 1197, 1929, 2586
Mva1269I GAATGC 2 cut(s) 26, 3404
MvaI CCWGG 3 cut(s) 471, 1684, 2223
MvnI CGCG 2 cut(s) 1692, 2432
NaeI GCCGGC 2 cut(s) 1609, 3160
NarI GGCGCC 1 cut(s) 1632
NciI CCSGG 1 cut(s) 9
NcoI CCATGG 2 cut(s) 3172, 3297
NdeI CATATG 1 cut(s) 3404
NgoMIV GCCGGC 2 cut(s) 1607, 3158
NlaIV GGNNCC 5 cut(s) 40, 1227, 1633, 1677, 2382
NmuCI GTSAC 9 cut(s) 103, 762, 1135, 1331, 1540, 1652, 2504, 3176, 3533
NsbI TGCGCA 1 cut(s) 1031
NsiI ATGCAT 3 cut(s) 751, 3404, 3479
NspI RCATGY 6 cut(s) 770, 922, 1076, 2317, 3477, 3683
OliI CACNNNNGTG 1 cut(s) 2730
PaeR7I CTCGAG 1 cut(s) 2720
PagI TCATGA 2 cut(s) 408, 3719
PaqCI CACCTGC 1 cut(s) 1582
PceI AGGCCT 1 cut(s) 1245
PciI ACATGT 2 cut(s) 766, 3679
PciSI GCTCTTC 1 cut(s) 2011
PctI GAATGC 2 cut(s) 26, 3404
PdiI GCCGGC 2 cut(s) 1609, 3160
PdmI GAANNNNTTC 3 cut(s) 24, 568, 2572
PflMI CCANNNNNTGG 1 cut(s) 215
PfoI TCCNGGA 1 cut(s) 7
PkrI GCNGC 7 cut(s) 796, 988, 1078, 1564, 2319, 3011, 3496
PleI GAGTC 2 cut(s) 395, 1256
PluTI GGCGCC 1 cut(s) 1635
PpsI GAGTC 2 cut(s) 395, 1256
PpuMI RGGWCCY 1 cut(s) 1226
PscI ACATGT 2 cut(s) 766, 3679
PshAI GACNNNNGTC 1 cut(s) 3341
PshBI ATTAAT 2 cut(s) 183, 1763
PsiI TTATAA 1 cut(s) 2267
Psp1406I AACGTT 2 cut(s) 1638, 2270
Psp5II RGGWCCY 1 cut(s) 1226
Psp6I CCWGG 3 cut(s) 469, 1682, 2221
PspEI GGTNACC 1 cut(s) 3176
PspFI CCCAGC 1 cut(s) 881
PspGI CCWGG 3 cut(s) 469, 1682, 2221
PspN4I GGNNCC 5 cut(s) 40, 1227, 1633, 1677, 2382
PspPI GGNCC 8 cut(s) 119, 146, 695, 716, 1098, 1226, 1668, 2090
PspPPI RGGWCCY 1 cut(s) 1226
PspXI VCTCGAGB 1 cut(s) 2720
PstNI CAGNNNCTG 1 cut(s) 986
PsuI RGATCY 4 cut(s) 2353, 2683, 2851, 3323
PvuII CAGCTG 1 cut(s) 1079
RsaI GTAC 5 cut(s) 371, 960, 2815, 2901, 3055
RsaNI GTAC 5 cut(s) 370, 959, 2814, 2900, 3054
RseI CAYNNNNRTG 6 cut(s) 141, 1220, 1338, 1626, 1772, 2730
SapI GCTCTTC 1 cut(s) 2011
SatI GCNGC 7 cut(s) 795, 987, 1077, 1563, 2318, 3010, 3495
Sau96I GGNCC 8 cut(s) 119, 146, 695, 716, 1098, 1226, 1668, 2090
ScaI AGTACT 1 cut(s) 2815
SchI GAGTC 2 cut(s) 396, 1256
ScrFI CCNGG 4 cut(s) 9, 471, 1684, 2223
SduI GDGCHC 4 cut(s) 777, 838, 2727, 2844
SfaNI GCATC 7 cut(s) 736, 758, 1133, 1500, 1507, 1927, 3229
SfcI CTRYAG 2 cut(s) 1701, 3082
SfoI GGCGCC 1 cut(s) 1633
Sfr274I CTCGAG 1 cut(s) 2720
SinI GGWCC 5 cut(s) 119, 146, 695, 716, 1226
SlaI CTCGAG 1 cut(s) 2720
SmiMI CAYNNNNRTG 6 cut(s) 141, 1220, 1338, 1626, 1772, 2730
SmlI CTYRAG 2 cut(s) 2720, 2837
SmoI CTYRAG 2 cut(s) 2720, 2837
SseBI AGGCCT 1 cut(s) 1245
SspDI GGCGCC 1 cut(s) 1631
SspI AATATT 4 cut(s) 181, 1068, 1798, 2657
SspMI CTAG 9 cut(s) 29, 90, 360, 812, 1014, 1317, 1665, 2357, 2630
StuI AGGCCT 1 cut(s) 1245
StyD4I CCNGG 4 cut(s) 7, 469, 1682, 2221
StyI CCWWGG 4 cut(s) 209, 2864, 3172, 3297
TaaI ACNGT 5 cut(s) 458, 1348, 2498, 2700, 2729
TaiI ACGT 6 cut(s) 760, 1500, 1641, 2158, 2273, 3187
TaqII GACCGA 1 cut(s) 3195
TatI WGTACW 2 cut(s) 958, 2813
TauI GCSGC 1 cut(s) 3012
TscAI CASTG 3 cut(s) 108, 1108, 2976
TseFI GTSAC 9 cut(s) 103, 762, 1135, 1331, 1540, 1652, 2504, 3176, 3533
TseI GCWGC 6 cut(s) 794, 986, 1076, 1562, 2317, 3494
Tsp45I GTSAC 9 cut(s) 103, 762, 1135, 1331, 1540, 1652, 2504, 3176, 3533
TspGWI ACGGA 8 cut(s) 336, 479, 525, 2440, 2497, 2844, 3117, 3134
TspRI CASTG 3 cut(s) 108, 1108, 2976
Van91I CCANNNNNTGG 1 cut(s) 215
VpaK11BI GGWCC 5 cut(s) 119, 146, 695, 716, 1226
VspI ATTAAT 2 cut(s) 183, 1763
XagI CCTNNNNNAGG 1 cut(s) 3264
XapI RAATTY 4 cut(s) 418, 1479, 1918, 3560
XbaI TCTAGA 3 cut(s) 28, 2356, 2629
XceI RCATGY 6 cut(s) 770, 922, 1076, 2317, 3477, 3683
XcmI CCANNNNNNNNNTGG 1 cut(s) 1136
XhoI CTCGAG 1 cut(s) 2720
XmnI GAANNNNTTC 3 cut(s) 24, 568, 2572
XspI CTAG 9 cut(s) 29, 90, 360, 812, 1014, 1317, 1665, 2357, 2630
ZrmI AGTACT 1 cut(s) 2815
Zsp2I ATGCAT 3 cut(s) 751, 3404, 3479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.