RchiOBHm_Chr7g0208771

Invertase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0002424
Physical Location & Seq
Reverse (-)
26091861 .. 26092343
483 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 483 bp
ATGCTTCTCAAAATGAGGTGTTCCTTCCTTGTCATTTTTCTCATGCTCTTTCACAACACAATCGATGATGATTTGATCCAACAATATTGCAAGAAAGCCGCAGATCACGAACCATCTGTGAACTACGACTTTTGTGTCTCAACCATTGAAGCCCACCCTAAAAGCCATAGTGCACACCTTGCAACACTTATAATGACTGTACTTGACCTAGCCGGAGCCAAGGTGACCAACTTCAATTCACACATTCAAGAGCTAAAGAAGGATCCAAAAGTTGACCAACCTACATTACAAGACTACGAAGAAGTATACTTAGATGCCACTAGTAGCATGCAGGAAGCTACCAATTCCTTCAAGGCCAAGAATTATGAGTCGACTCTTAATACTCTTAGTGCTGCTATGAATGCACCTGATACTTGTGAAACTGGATTTCAAGAGAGGAAAGCAGTGTCTCCCTTGAAGAAGGAGGACGATGACTTCACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

160

Amino Acids

18.06

Weight (kDa)

5.01

Isoelectric Point (pI)

24.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 24 - 154 2.6e-21 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 191
AasI GACNNNNNNGTC 1 cut(s) 134
AccI GTMKAC 2 cut(s) 306, 371
AciI CCGC 1 cut(s) 99
AclWI GGATC 3 cut(s) 70, 257, 270
AfaI GTAC 1 cut(s) 201
AgsI TTSAA 6 cut(s) 149, 235, 248, 352, 431, 457
AhlI ACTAGT 1 cut(s) 320
AluBI AGCT 2 cut(s) 253, 338
AluI AGCT 2 cut(s) 253, 338
Alw21I GWGCWC 1 cut(s) 175
Alw26I GTCTC 2 cut(s) 142, 453
Alw44I GTGCAC 1 cut(s) 171
AlwI GGATC 3 cut(s) 70, 257, 270
AoxI GGCC 1 cut(s) 354
ApaLI GTGCAC 1 cut(s) 171
ApeKI GCWGC 1 cut(s) 392
AsuHPI GGTGA 1 cut(s) 235
BaeGI GKGCMC 1 cut(s) 175
BamHI GGATCC 1 cut(s) 262
Bbv12I GWGCWC 1 cut(s) 175
BbvI GCAGC 1 cut(s) 379
BccI CCATC 1 cut(s) 121
BcoDI GTCTC 2 cut(s) 142, 453
BcuI ACTAGT 1 cut(s) 320
BfaI CTAG 2 cut(s) 209, 321
BisI GCNGC 2 cut(s) 99, 393
BlsI GCNGC 2 cut(s) 100, 394
BmiI GGNNCC 2 cut(s) 217, 264
BmsI GCATC 1 cut(s) 304
Bsa29I ATCGAT 1 cut(s) 63
BsaJI CCNNGG 1 cut(s) 219
BsaXI ACNNNNNCTCC 2 cut(s) 207, 237
Bse1I ACTGG 1 cut(s) 427
BseCI ATCGAT 1 cut(s) 63
BseDI CCNNGG 1 cut(s) 219
BseNI ACTGG 1 cut(s) 427
BseSI GKGCMC 1 cut(s) 175
BseXI GCAGC 1 cut(s) 379
BshFI GGCC 1 cut(s) 356
BshVI ATCGAT 1 cut(s) 63
BsiHKAI GWGCWC 1 cut(s) 175
BsiSI CCGG 1 cut(s) 213
BsmAI GTCTC 2 cut(s) 142, 453
BsmI GAATGC 1 cut(s) 406
BsnI GGCC 1 cut(s) 356
Bsp1286I GDGCHC 1 cut(s) 175
Bsp143I GATC 3 cut(s) 75, 103, 262
BspACI CCGC 1 cut(s) 99
BspANI GGCC 1 cut(s) 356
BspDI ATCGAT 1 cut(s) 63
BspLI GGNNCC 2 cut(s) 217, 264
BspPI GGATC 3 cut(s) 70, 257, 270
BsrI ACTGG 1 cut(s) 427
BssECI CCNNGG 1 cut(s) 219
BssMI GATC 3 cut(s) 75, 103, 262
BssNAI GTATAC 1 cut(s) 307
BssT1I CCWWGG 1 cut(s) 219
Bst1107I GTATAC 1 cut(s) 307
Bst4CI ACNGT 1 cut(s) 199
BstAPI GCANNNNNTGC 1 cut(s) 179
BstC8I GCNNGC 1 cut(s) 329
BstDEI CTNAG 2 cut(s) 310, 386
BstEII GGTNACC 1 cut(s) 223
BstKTI GATC 3 cut(s) 78, 106, 265
BstMAI GTCTC 2 cut(s) 142, 453
BstMBI GATC 3 cut(s) 75, 103, 262
BstMWI GCNNNNNNNGC 2 cut(s) 179, 401
BstNSI RCATGY 1 cut(s) 331
BstPI GGTNACC 1 cut(s) 223
BstSLI GKGCMC 1 cut(s) 175
BstV1I GCAGC 1 cut(s) 379
BstX2I RGATCY 1 cut(s) 262
BstYI RGATCY 1 cut(s) 262
BstZ17I GTATAC 1 cut(s) 307
Bsu15I ATCGAT 1 cut(s) 63
BsuRI GGCC 1 cut(s) 356
BsuTUI ATCGAT 1 cut(s) 63
BtsI GCAGTG 1 cut(s) 450
BtsIMutI CAGTG 1 cut(s) 450
Cac8I GCNNGC 1 cut(s) 329
ClaI ATCGAT 1 cut(s) 63
Csp6I GTAC 1 cut(s) 200
CviAII CATG 2 cut(s) 43, 328
CviJI RGCY 8 cut(s) 98, 152, 165, 212, 218, 253, 338, 356
CviKI_1 RGCY 8 cut(s) 98, 152, 165, 212, 218, 253, 338, 356
CviQI GTAC 1 cut(s) 200
DdeI CTNAG 2 cut(s) 310, 386
DpnI GATC 3 cut(s) 77, 105, 264
DpnII GATC 3 cut(s) 75, 103, 262
DrdI GACNNNNNNGTC 1 cut(s) 134
DseDI GACNNNNNNGTC 1 cut(s) 134
Eco130I CCWWGG 1 cut(s) 219
Eco91I GGTNACC 1 cut(s) 223
EcoO65I GGTNACC 1 cut(s) 223
EcoT14I CCWWGG 1 cut(s) 219
ErhI CCWWGG 1 cut(s) 219
FaeI CATG 2 cut(s) 46, 331
FaiI YATR 7 cut(s) 44, 168, 191, 307, 329, 366, 398
FatI CATG 2 cut(s) 42, 327
FblI GTMKAC 2 cut(s) 306, 371
Fnu4HI GCNGC 2 cut(s) 99, 393
Fsp4HI GCNGC 2 cut(s) 99, 393
FspBI CTAG 2 cut(s) 209, 321
GluI GCNGC 2 cut(s) 99, 393
HaeIII GGCC 1 cut(s) 356
HapII CCGG 1 cut(s) 213
Hin1II CATG 2 cut(s) 46, 331
HincII GTYRAC 2 cut(s) 274, 372
HindII GTYRAC 2 cut(s) 274, 372
HinfI GANTC 2 cut(s) 368, 373
HpaII CCGG 1 cut(s) 213
HphI GGTGA 1 cut(s) 235
Hpy166II GTNNAC 5 cut(s) 121, 173, 274, 307, 372
Hpy188III TCNNGA 3 cut(s) 107, 248, 431
Hpy8I GTNNAC 5 cut(s) 121, 173, 274, 307, 372
HpyAV CCTTC 4 cut(s) 34, 253, 358, 454
HpyCH4III ACNGT 1 cut(s) 199
HpyCH4V TGCA 5 cut(s) 90, 173, 182, 331, 404
HpyF10VI GCNNNNNNNGC 2 cut(s) 179, 401
HpyF3I CTNAG 2 cut(s) 310, 386
Hsp92II CATG 2 cut(s) 46, 331
Kzo9I GATC 3 cut(s) 75, 103, 262
LmnI GCTCC 1 cut(s) 215
LpnPI CCDG 4 cut(s) 226, 317, 408, 420
Lsp1109I GCAGC 1 cut(s) 379
LweI GCATC 1 cut(s) 304
MaeI CTAG 2 cut(s) 209, 321
MaeIII GTNAC 1 cut(s) 223
MalI GATC 3 cut(s) 77, 105, 264
MboI GATC 3 cut(s) 75, 103, 262
MboII GAAGA 2 cut(s) 311, 469
MflI RGATCY 1 cut(s) 262
MhlI GDGCHC 1 cut(s) 175
MluCI AATT 3 cut(s) 235, 343, 361
MlyI GAGTC 2 cut(s) 367, 377
MmeI TCCRAC 1 cut(s) 103
MnlI CCTC 3 cut(s) 9, 429, 457
MseI TTAA 2 cut(s) 378, 481
MspI CCGG 1 cut(s) 213
Mva1269I GAATGC 1 cut(s) 406
MwoI GCNNNNNNNGC 2 cut(s) 179, 401
NdeII GATC 3 cut(s) 75, 103, 262
NlaIII CATG 2 cut(s) 46, 331
NlaIV GGNNCC 2 cut(s) 217, 264
NmuCI GTSAC 1 cut(s) 223
NspI RCATGY 1 cut(s) 331
PaeI GCATGC 1 cut(s) 331
PctI GAATGC 1 cut(s) 406
PkrI GCNGC 2 cut(s) 100, 394
PleI GAGTC 2 cut(s) 367, 376
PpsI GAGTC 2 cut(s) 367, 376
PsiI TTATAA 1 cut(s) 191
PspEI GGTNACC 1 cut(s) 223
PspN4I GGNNCC 2 cut(s) 217, 264
PsuI RGATCY 1 cut(s) 262
RsaI GTAC 1 cut(s) 201
RsaNI GTAC 1 cut(s) 200
SalI GTCGAC 1 cut(s) 370
SaqAI TTAA 2 cut(s) 378, 481
SatI GCNGC 2 cut(s) 99, 393
Sau3AI GATC 3 cut(s) 75, 103, 262
SchI GAGTC 2 cut(s) 367, 377
SduI GDGCHC 1 cut(s) 175
SetI ASST 8 cut(s) 20, 180, 210, 225, 255, 283, 340, 409
SfaNI GCATC 1 cut(s) 304
SpeI ACTAGT 1 cut(s) 320
SphI GCATGC 1 cut(s) 331
Sse9I AATT 3 cut(s) 235, 343, 361
SsiI CCGC 1 cut(s) 99
SspI AATATT 1 cut(s) 86
SspMI CTAG 2 cut(s) 209, 321
StyI CCWWGG 1 cut(s) 219
TaaI ACNGT 1 cut(s) 199
TaqI TCGA 2 cut(s) 63, 371
TasI AATT 3 cut(s) 235, 343, 361
TatI WGTACW 1 cut(s) 199
TauI GCSGC 1 cut(s) 101
Tru1I TTAA 2 cut(s) 378, 481
Tru9I TTAA 2 cut(s) 378, 481
TscAI CASTG 1 cut(s) 450
TseFI GTSAC 1 cut(s) 223
TseI GCWGC 1 cut(s) 392
Tsp45I GTSAC 1 cut(s) 223
TspDTI ATGAA 1 cut(s) 413
TspRI CASTG 1 cut(s) 450
VneI GTGCAC 1 cut(s) 171
XceI RCATGY 1 cut(s) 331
XmiI GTMKAC 2 cut(s) 306, 371
XspI CTAG 2 cut(s) 209, 321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.