Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1628 |
| Acc65I | GGTACC | 1 cut(s) | 2202 |
| AccB1I | GGYRCC | 1 cut(s) | 2202 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 689 |
| AccI | GTMKAC | 3 cut(s) | 465, 573, 1494 |
| AccII | CGCG | 1 cut(s) | 495 |
| AccIII | TCCGGA | 1 cut(s) | 1568 |
| AciI | CCGC | 6 cut(s) | 493, 663, 1802, 1868, 1953, 2215 |
| AclI | AACGTT | 1 cut(s) | 1421 |
| AclWI | GGATC | 4 cut(s) | 647, 660, 749, 1199 |
| AcoI | YGGCCR | 2 cut(s) | 680, 1143 |
| AcsI | RAATTY | 4 cut(s) | 1124, 1181, 2352, 2589 |
| AcuI | CTGAAG | 4 cut(s) | 1925, 2139, 2306, 2531 |
| AdeI | CACNNNGTG | 1 cut(s) | 1612 |
| AfaI | GTAC | 6 cut(s) | 411, 707, 803, 948, 2189, 2204 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 431, 662, 689, 722, 771, 2198, 2249, 2290 |
| AflII | CTTAAG | 1 cut(s) | 577 |
| AflIII | ACRYGT | 2 cut(s) | 798, 1763 |
| AgeI | ACCGGT | 1 cut(s) | 2527 |
| AgsI | TTSAA | 7 cut(s) | 52, 127, 235, 1426, 1600, 2302, 2486 |
| AhlI | ACTAGT | 1 cut(s) | 2561 |
| AjnI | CCWGG | 3 cut(s) | 352, 1721, 2360 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 333, 365 |
| AleI | CACNNNNGTG | 1 cut(s) | 536 |
| Alw21I | GWGCWC | 2 cut(s) | 603, 2274 |
| Alw26I | GTCTC | 3 cut(s) | 297, 1178, 2408 |
| AlwI | GGATC | 4 cut(s) | 647, 660, 749, 1199 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 2329 |
| Ama87I | CYCGRG | 3 cut(s) | 647, 1080, 2405 |
| Aor13HI | TCCGGA | 1 cut(s) | 1568 |
| AoxI | GGCC | 3 cut(s) | 680, 1143, 2093 |
| ApeKI | GCWGC | 5 cut(s) | 190, 296, 1479, 1645, 2168 |
| ApoI | RAATTY | 4 cut(s) | 1124, 1181, 2352, 2589 |
| ArsI | GACNNNNNNTTYG | 8 cut(s) | 296, 328, 660, 692, 1907, 1939, 2106, 2138 |
| AseI | ATTAAT | 1 cut(s) | 1710 |
| AsiGI | ACCGGT | 1 cut(s) | 2527 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 420, 1182 |
| Asp718I | GGTACC | 1 cut(s) | 2202 |
| AspS9I | GGNCC | 4 cut(s) | 1271, 1950, 2011, 2525 |
| AsuC2I | CCSGG | 1 cut(s) | 426 |
| AsuHPI | GGTGA | 5 cut(s) | 1064, 1232, 1652, 2081, 2542 |
| AsuNHI | GCTAGC | 1 cut(s) | 1385 |
| AvaI | CYCGRG | 3 cut(s) | 647, 1080, 2405 |
| AvaII | GGWCC | 4 cut(s) | 1271, 1950, 2011, 2525 |
| AxyI | CCTNAGG | 1 cut(s) | 1064 |
| BamHI | GGATCC | 1 cut(s) | 652 |
| BanI | GGYRCC | 1 cut(s) | 2202 |
| BanII | GRGCYC | 2 cut(s) | 603, 2274 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 2525, 2557 |
| BbsI | GAAGAC | 1 cut(s) | 1889 |
| Bbv12I | GWGCWC | 2 cut(s) | 603, 2274 |
| BbvI | GCAGC | 5 cut(s) | 202, 308, 1466, 1657, 2180 |
| BccI | CCATC | 8 cut(s) | 38, 581, 1043, 1262, 1329, 1409, 1780, 1928 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 394, 428 |
| BciT130I | CCWGG | 3 cut(s) | 354, 1723, 2362 |
| BciVI | GTATCC | 1 cut(s) | 1357 |
| BclI | TGATCA | 1 cut(s) | 1222 |
| BcnI | CCSGG | 1 cut(s) | 426 |
| BcoDI | GTCTC | 3 cut(s) | 297, 1178, 2408 |
| BcuI | ACTAGT | 1 cut(s) | 2561 |
| BfmI | CTRYAG | 3 cut(s) | 360, 981, 1842 |
| BfrI | CTTAAG | 1 cut(s) | 577 |
| BfuI | GTATCC | 1 cut(s) | 1357 |
| BglII | AGATCT | 2 cut(s) | 1377, 1846 |
| BisI | GCNGC | 6 cut(s) | 191, 297, 664, 1480, 1646, 2169 |
| BlpI | GCTNAGC | 1 cut(s) | 2316 |
| BlsI | GCNGC | 6 cut(s) | 192, 298, 665, 1481, 1647, 2170 |
| Bme1390I | CCNGG | 4 cut(s) | 354, 426, 1723, 2362 |
| Bme18I | GGWCC | 4 cut(s) | 1271, 1950, 2011, 2525 |
| BmeT110I | CYCGRG | 3 cut(s) | 647, 1080, 2405 |
| BmgT120I | GGNCC | 4 cut(s) | 1271, 1950, 2011, 2525 |
| BmiI | GGNNCC | 4 cut(s) | 654, 1070, 2204, 2495 |
| BmrFI | CCNGG | 4 cut(s) | 354, 426, 1723, 2362 |
| BmrI | ACTGGG | 3 cut(s) | 741, 1484, 1771 |
| BmsI | GCATC | 5 cut(s) | 414, 901, 1108, 1326, 2526 |
| BmtI | GCTAGC | 1 cut(s) | 1389 |
| BmuI | ACTGGG | 3 cut(s) | 741, 1484, 1771 |
| BpiI | GAAGAC | 1 cut(s) | 1889 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1438, 1470 |
| BpmI | CTGGAG | 2 cut(s) | 375, 2015 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1827 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 2316 |
| BpuEI | CTTGAG | 7 cut(s) | 383, 587, 861, 1013, 1426, 1838, 2258 |
| BpuMI | CCSGG | 1 cut(s) | 426 |
| BsaWI | WCCGGW | 2 cut(s) | 1568, 2527 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 11, 41, 348, 378, 1854, 1884 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 431, 662, 689, 722, 771, 2198, 2249, 2290 |
| Bse118I | RCCGGY | 1 cut(s) | 2527 |
| Bse21I | CCTNAGG | 1 cut(s) | 1064 |
| BseAI | TCCGGA | 1 cut(s) | 1568 |
| BseBI | CCWGG | 3 cut(s) | 354, 1723, 2362 |
| BseGI | GGATG | 9 cut(s) | 30, 279, 340, 444, 1123, 1456, 1681, 1772, 1882 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 431, 662, 689, 722, 771, 2198, 2249, 2290 |
| BseMII | CTCAG | 4 cut(s) | 1078, 1640, 1818, 2330 |
| BseRI | GAGGAG | 6 cut(s) | 597, 1298, 1721, 1841, 1875, 2282 |
| BseXI | GCAGC | 5 cut(s) | 202, 308, 1466, 1657, 2180 |
| BseYI | CCCAGC | 1 cut(s) | 891 |
| BsgI | GTGCAG | 2 cut(s) | 209, 1465 |
| Bsh1236I | CGCG | 1 cut(s) | 495 |
| BshFI | GGCC | 3 cut(s) | 682, 1145, 2095 |
| BshNI | GGYRCC | 1 cut(s) | 2202 |
| BshTI | ACCGGT | 1 cut(s) | 2527 |
| BsiHKAI | GWGCWC | 2 cut(s) | 603, 2274 |
| BsiHKCI | CYCGRG | 3 cut(s) | 647, 1080, 2405 |
| BsiSI | CCGG | 6 cut(s) | 63, 425, 679, 1146, 1569, 2528 |
| BslFI | GGGAC | 3 cut(s) | 626, 998, 2411 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 431, 662, 689, 722, 771, 2198, 2249, 2290 |
| BsmAI | GTCTC | 3 cut(s) | 297, 1178, 2408 |
| BsmBI | CGTCTC | 1 cut(s) | 1178 |
| BsmFI | GGGAC | 3 cut(s) | 626, 998, 2411 |
| BsmI | GAATGC | 1 cut(s) | 311 |
| BsnI | GGCC | 3 cut(s) | 682, 1145, 2095 |
| BsoBI | CYCGRG | 3 cut(s) | 647, 1080, 2405 |
| Bsp1286I | GDGCHC | 2 cut(s) | 603, 2274 |
| Bsp13I | TCCGGA | 1 cut(s) | 1568 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 2316 |
| BspACI | CCGC | 6 cut(s) | 493, 663, 1802, 1868, 1953, 2215 |
| BspANI | GGCC | 3 cut(s) | 682, 1145, 2095 |
| BspCNI | CTCAG | 4 cut(s) | 1077, 1641, 1819, 2329 |
| BspEI | TCCGGA | 1 cut(s) | 1568 |
| BspFNI | CGCG | 1 cut(s) | 495 |
| BspHI | TCATGA | 1 cut(s) | 1324 |
| BspLI | GGNNCC | 4 cut(s) | 654, 1070, 2204, 2495 |
| BspMAI | CTGCAG | 1 cut(s) | 1846 |
| BspOI | GCTAGC | 1 cut(s) | 1389 |
| BspPI | GGATC | 4 cut(s) | 647, 660, 749, 1199 |
| BspT107I | GGYRCC | 1 cut(s) | 2202 |
| BspTI | CTTAAG | 1 cut(s) | 577 |
| BsrFI | RCCGGY | 1 cut(s) | 2527 |
| BssAI | RCCGGY | 1 cut(s) | 2527 |
| BssNAI | GTATAC | 1 cut(s) | 1495 |
| Bst1107I | GTATAC | 1 cut(s) | 1495 |
| Bst2UI | CCWGG | 3 cut(s) | 354, 1723, 2362 |
| Bst4CI | ACNGT | 7 cut(s) | 157, 1895, 2069, 2187, 2224, 2402, 2600 |
| Bst6I | CTCTTC | 2 cut(s) | 555, 1968 |
| BstAFI | CTTAAG | 1 cut(s) | 577 |
| BstC8I | GCNNGC | 6 cut(s) | 255, 1199, 1387, 1451, 1833, 2367 |
| BstDEI | CTNAG | 6 cut(s) | 793, 1064, 1368, 1649, 1827, 2316 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 720, 2196 |
| BstF5I | GGATG | 9 cut(s) | 30, 279, 340, 444, 1123, 1456, 1681, 1772, 1882 |
| BstFNI | CGCG | 1 cut(s) | 495 |
| BstMAI | GTCTC | 3 cut(s) | 297, 1178, 2408 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 1485, 1553, 1654, 1841, 2092, 2101, 2165 |
| BstNI | CCWGG | 3 cut(s) | 354, 1723, 2362 |
| BstNSI | RCATGY | 4 cut(s) | 802, 1767, 2133, 2369 |
| BstSCI | CCNGG | 4 cut(s) | 352, 424, 1721, 2360 |
| BstSFI | CTRYAG | 3 cut(s) | 360, 981, 1842 |
| BstUI | CGCG | 1 cut(s) | 495 |
| BstV1I | GCAGC | 5 cut(s) | 202, 308, 1466, 1657, 2180 |
| BstV2I | GAAGAC | 1 cut(s) | 1889 |
| BstX2I | RGATCY | 5 cut(s) | 652, 754, 1204, 1377, 1846 |
| BstYI | RGATCY | 5 cut(s) | 652, 754, 1204, 1377, 1846 |
| BstZ17I | GTATAC | 1 cut(s) | 1495 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1064 |
| BsuI | GTATCC | 1 cut(s) | 1357 |
| BsuRI | GGCC | 3 cut(s) | 682, 1145, 2095 |
| BtsCI | GGATG | 9 cut(s) | 30, 279, 340, 444, 1123, 1456, 1681, 1772, 1882 |
| BtsI | GCAGTG | 1 cut(s) | 1617 |
| BtsIMutI | CAGTG | 6 cut(s) | 49, 543, 1430, 1591, 1617, 2220 |
| Cac8I | GCNNGC | 6 cut(s) | 255, 1199, 1387, 1451, 1833, 2367 |
| CaiI | CAGNNNCTG | 1 cut(s) | 2329 |
| CciI | TCATGA | 1 cut(s) | 1324 |
| Cfr10I | RCCGGY | 1 cut(s) | 2527 |
| Cfr13I | GGNCC | 4 cut(s) | 1271, 1950, 2011, 2525 |
| Csp6I | GTAC | 6 cut(s) | 410, 706, 802, 947, 2188, 2203 |
| CspAI | ACCGGT | 1 cut(s) | 2527 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1726, 1761 |
| CviQI | GTAC | 6 cut(s) | 410, 706, 802, 947, 2188, 2203 |
| DdeI | CTNAG | 6 cut(s) | 793, 1064, 1368, 1649, 1827, 2316 |
| DraIII | CACNNNGTG | 1 cut(s) | 1612 |
| EaeI | YGGCCR | 2 cut(s) | 680, 1143 |
| Eam1104I | CTCTTC | 2 cut(s) | 555, 1968 |
| EarI | CTCTTC | 2 cut(s) | 555, 1968 |
| Ecl136II | GAGCTC | 2 cut(s) | 601, 2272 |
| Eco24I | GRGCYC | 2 cut(s) | 603, 2274 |
| Eco32I | GATATC | 1 cut(s) | 1574 |
| Eco47I | GGWCC | 4 cut(s) | 1271, 1950, 2011, 2525 |
| Eco53kI | GAGCTC | 2 cut(s) | 601, 2272 |
| Eco57I | CTGAAG | 4 cut(s) | 1925, 2139, 2306, 2531 |
| Eco81I | CCTNAGG | 1 cut(s) | 1064 |
| Eco88I | CYCGRG | 3 cut(s) | 647, 1080, 2405 |
| EcoICRI | GAGCTC | 2 cut(s) | 601, 2272 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 720, 2196 |
| EcoRI | GAATTC | 1 cut(s) | 2589 |
| EcoRII | CCWGG | 3 cut(s) | 352, 1721, 2360 |
| EcoRV | GATATC | 1 cut(s) | 1574 |
| EcoT22I | ATGCAT | 1 cut(s) | 1786 |
| EcoT38I | GRGCYC | 2 cut(s) | 603, 2274 |
| Esp3I | CGTCTC | 1 cut(s) | 1178 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 2282, 2314, 2559, 2591 |
| FaqI | GGGAC | 3 cut(s) | 626, 998, 2411 |
| FauNDI | CATATG | 3 cut(s) | 1342, 1529, 2144 |
| FbaI | TGATCA | 1 cut(s) | 1222 |
| FblI | GTMKAC | 3 cut(s) | 465, 573, 1494 |
| Fnu4HI | GCNGC | 6 cut(s) | 191, 297, 664, 1480, 1646, 2169 |
| FokI | GGATG | 9 cut(s) | 17, 286, 347, 451, 1130, 1443, 1668, 1759, 1889 |
| FriOI | GRGCYC | 2 cut(s) | 603, 2274 |
| Fsp4HI | GCNGC | 6 cut(s) | 191, 297, 664, 1480, 1646, 2169 |
| GluI | GCNGC | 6 cut(s) | 191, 297, 664, 1480, 1646, 2169 |
| GsaI | CCCAGC | 1 cut(s) | 895 |
| GsuI | CTGGAG | 2 cut(s) | 375, 2015 |
| HaeIII | GGCC | 3 cut(s) | 682, 1145, 2095 |
| HapII | CCGG | 6 cut(s) | 63, 425, 679, 1146, 1569, 2528 |
| HincII | GTYRAC | 1 cut(s) | 2176 |
| HindII | GTYRAC | 1 cut(s) | 2176 |
| HindIII | AAGCTT | 3 cut(s) | 900, 1522, 1545 |
| HinfI | GANTC | 7 cut(s) | 172, 305, 1328, 1393, 1565, 2107, 2235 |
| HpaII | CCGG | 6 cut(s) | 63, 425, 679, 1146, 1569, 2528 |
| HphI | GGTGA | 5 cut(s) | 1064, 1232, 1652, 2081, 2542 |
| Hpy166II | GTNNAC | 9 cut(s) | 466, 574, 1055, 1153, 1495, 1807, 1889, 2176, 2412 |
| Hpy188I | TCNGA | 7 cut(s) | 438, 844, 1043, 1944, 2325, 2511, 2612 |
| Hpy8I | GTNNAC | 9 cut(s) | 466, 574, 1055, 1153, 1495, 1807, 1889, 2176, 2412 |
| Hpy99I | CGWCG | 1 cut(s) | 1173 |
| HpyAV | CCTTC | 7 cut(s) | 956, 1409, 2038, 2053, 2216, 2243, 2564 |
| HpyCH4III | ACNGT | 7 cut(s) | 157, 1895, 2069, 2187, 2224, 2402, 2600 |
| HpyCH4IV | ACGT | 1 cut(s) | 1421 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 1485, 1553, 1654, 1841, 2092, 2101, 2165 |
| HpyF3I | CTNAG | 6 cut(s) | 793, 1064, 1368, 1649, 1827, 2316 |
| HpySE526I | ACGT | 1 cut(s) | 1421 |
| Kpn2I | TCCGGA | 1 cut(s) | 1568 |
| KpnI | GGTACC | 1 cut(s) | 2206 |
| Ksp22I | TGATCA | 1 cut(s) | 1222 |
| LmnI | GCTCC | 3 cut(s) | 356, 811, 2269 |
| Lsp1109I | GCAGC | 5 cut(s) | 202, 308, 1466, 1657, 2180 |
| LweI | GCATC | 5 cut(s) | 414, 901, 1108, 1326, 2526 |
| MaeII | ACGT | 1 cut(s) | 1421 |
| MaeIII | GTNAC | 5 cut(s) | 527, 1589, 1694, 2063, 2069 |
| MflI | RGATCY | 5 cut(s) | 652, 754, 1204, 1377, 1846 |
| MhlI | GDGCHC | 2 cut(s) | 603, 2274 |
| MlyI | GAGTC | 5 cut(s) | 181, 299, 1387, 1559, 2101 |
| MmeI | TCCRAC | 3 cut(s) | 416, 867, 2503 |
| Mph1103I | ATGCAT | 1 cut(s) | 1786 |
| MroI | TCCGGA | 1 cut(s) | 1568 |
| MroXI | GAANNNNTTC | 2 cut(s) | 420, 1182 |
| MseI | TTAA | 7 cut(s) | 6, 578, 833, 992, 1238, 1710, 2075 |
| MslI | CAYNNNNRTG | 4 cut(s) | 185, 536, 1692, 2370 |
| MspA1I | CMGCKG | 3 cut(s) | 1505, 1648, 2171 |
| MspCI | CTTAAG | 1 cut(s) | 577 |
| MspI | CCGG | 6 cut(s) | 63, 425, 679, 1146, 1569, 2528 |
| MspR9I | CCNGG | 4 cut(s) | 354, 426, 1723, 2362 |
| Mva1269I | GAATGC | 1 cut(s) | 311 |
| MvaI | CCWGG | 3 cut(s) | 354, 1723, 2362 |
| MvnI | CGCG | 1 cut(s) | 495 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 1485, 1553, 1654, 1841, 2092, 2101, 2165 |
| NciI | CCSGG | 1 cut(s) | 426 |
| NdeI | CATATG | 3 cut(s) | 1342, 1529, 2144 |
| NheI | GCTAGC | 1 cut(s) | 1385 |
| NlaIV | GGNNCC | 4 cut(s) | 654, 1070, 2204, 2495 |
| NmeAIII | GCCGAG | 1 cut(s) | 2313 |
| NmuCI | GTSAC | 5 cut(s) | 527, 1589, 1694, 2063, 2069 |
| NsiI | ATGCAT | 1 cut(s) | 1786 |
| NspI | RCATGY | 4 cut(s) | 802, 1767, 2133, 2369 |
| OliI | CACNNNNGTG | 1 cut(s) | 536 |
| PaeI | GCATGC | 1 cut(s) | 2369 |
| PaeR7I | CTCGAG | 2 cut(s) | 1080, 2405 |
| PagI | TCATGA | 1 cut(s) | 1324 |
| PciI | ACATGT | 2 cut(s) | 798, 1763 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1614 |
| PctI | GAATGC | 1 cut(s) | 311 |
| PdmI | GAANNNNTTC | 2 cut(s) | 420, 1182 |
| PfeI | GAWTC | 2 cut(s) | 1328, 2235 |
| PflFI | GACNNNGTC | 2 cut(s) | 2009, 2120 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 689 |
| PfoI | TCCNGGA | 1 cut(s) | 352 |
| PinAI | ACCGGT | 1 cut(s) | 2527 |
| PkrI | GCNGC | 6 cut(s) | 192, 298, 665, 1481, 1647, 2170 |
| PleI | GAGTC | 5 cut(s) | 180, 299, 1387, 1559, 2101 |
| PpsI | GAGTC | 5 cut(s) | 180, 299, 1387, 1559, 2101 |
| PscI | ACATGT | 2 cut(s) | 798, 1763 |
| PshBI | ATTAAT | 1 cut(s) | 1710 |
| PsiI | TTATAA | 1 cut(s) | 1628 |
| Psp124BI | GAGCTC | 2 cut(s) | 603, 2274 |
| Psp1406I | AACGTT | 1 cut(s) | 1421 |
| Psp6I | CCWGG | 3 cut(s) | 352, 1721, 2360 |
| PspFI | CCCAGC | 1 cut(s) | 891 |
| PspGI | CCWGG | 3 cut(s) | 352, 1721, 2360 |
| PspN4I | GGNNCC | 4 cut(s) | 654, 1070, 2204, 2495 |
| PspPI | GGNCC | 4 cut(s) | 1271, 1950, 2011, 2525 |
| PspXI | VCTCGAGB | 1 cut(s) | 1080 |
| PstI | CTGCAG | 1 cut(s) | 1846 |
| PstNI | CAGNNNCTG | 1 cut(s) | 2329 |
| PsuI | RGATCY | 5 cut(s) | 652, 754, 1204, 1377, 1846 |
| PsyI | GACNNNGTC | 2 cut(s) | 2009, 2120 |
| PvuII | CAGCTG | 3 cut(s) | 1505, 1648, 2171 |
| RsaI | GTAC | 6 cut(s) | 411, 707, 803, 948, 2189, 2204 |
| RsaNI | GTAC | 6 cut(s) | 410, 706, 802, 947, 2188, 2203 |
| RseI | CAYNNNNRTG | 4 cut(s) | 185, 536, 1692, 2370 |
| SacI | GAGCTC | 2 cut(s) | 603, 2274 |
| SaqAI | TTAA | 7 cut(s) | 6, 578, 833, 992, 1238, 1710, 2075 |
| SatI | GCNGC | 6 cut(s) | 191, 297, 664, 1480, 1646, 2169 |
| Sau96I | GGNCC | 4 cut(s) | 1271, 1950, 2011, 2525 |
| SchI | GAGTC | 5 cut(s) | 181, 299, 1387, 1559, 2101 |
| ScrFI | CCNGG | 4 cut(s) | 354, 426, 1723, 2362 |
| SduI | GDGCHC | 2 cut(s) | 603, 2274 |
| SfaNI | GCATC | 5 cut(s) | 414, 901, 1108, 1326, 2526 |
| SfcI | CTRYAG | 3 cut(s) | 360, 981, 1842 |
| Sfr274I | CTCGAG | 2 cut(s) | 1080, 2405 |
| SinI | GGWCC | 4 cut(s) | 1271, 1950, 2011, 2525 |
| SlaI | CTCGAG | 2 cut(s) | 1080, 2405 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 185, 536, 1692, 2370 |
| SpeI | ACTAGT | 1 cut(s) | 2561 |
| SphI | GCATGC | 1 cut(s) | 2369 |
| SsiI | CCGC | 6 cut(s) | 493, 663, 1802, 1868, 1953, 2215 |
| SspI | AATATT | 1 cut(s) | 1708 |
| SstI | GAGCTC | 2 cut(s) | 603, 2274 |
| StyD4I | CCNGG | 4 cut(s) | 352, 424, 1721, 2360 |
| TaaI | ACNGT | 7 cut(s) | 157, 1895, 2069, 2187, 2224, 2402, 2600 |
| TaiI | ACGT | 1 cut(s) | 1424 |
| TatI | WGTACW | 1 cut(s) | 946 |
| TauI | GCSGC | 1 cut(s) | 666 |
| TfiI | GAWTC | 2 cut(s) | 1328, 2235 |
| Tru1I | TTAA | 7 cut(s) | 6, 578, 833, 992, 1238, 1710, 2075 |
| Tru9I | TTAA | 7 cut(s) | 6, 578, 833, 992, 1238, 1710, 2075 |
| TscAI | CASTG | 6 cut(s) | 49, 543, 1437, 1598, 1617, 2227 |
| TseFI | GTSAC | 5 cut(s) | 527, 1589, 1694, 2063, 2069 |
| TseI | GCWGC | 5 cut(s) | 190, 296, 1479, 1645, 2168 |
| Tsp45I | GTSAC | 5 cut(s) | 527, 1589, 1694, 2063, 2069 |
| TspGWI | ACGGA | 3 cut(s) | 175, 1130, 1682 |
| TspRI | CASTG | 6 cut(s) | 49, 543, 1437, 1598, 1617, 2227 |
| Tth111I | GACNNNGTC | 2 cut(s) | 2009, 2120 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 689 |
| Vha464I | CTTAAG | 1 cut(s) | 577 |
| VpaK11BI | GGWCC | 4 cut(s) | 1271, 1950, 2011, 2525 |
| VspI | ATTAAT | 1 cut(s) | 1710 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 720, 2196 |
| XapI | RAATTY | 4 cut(s) | 1124, 1181, 2352, 2589 |
| XceI | RCATGY | 4 cut(s) | 802, 1767, 2133, 2369 |
| XhoI | CTCGAG | 2 cut(s) | 1080, 2405 |
| XmiI | GTMKAC | 3 cut(s) | 465, 573, 1494 |
| XmnI | GAANNNNTTC | 2 cut(s) | 420, 1182 |
| Zsp2I | ATGCAT | 1 cut(s) | 1786 |