Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0003802

Physical Location & Seq

Genomic Coordinates Forward (+)

37480650 .. 37485619

Gene Structure 4970 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3591 bp

ATGGGCCATTGCATGAAAAGGACTCTGTCTTTGAAGCAGCCGCCGATTTTGGCTCAGTCCAAACCCTCCTCCTATTCGTCGAAACCGAACAACTTCCGCTTCCTATACTCCAAGAAGCTCGGCGCCCTACCGTCGCTCAGCCTCCGGTCATGGCCGCAGTCGCAGAGTCATCGTCCGAGTTGCCGAGTCAACTCGGTTCGGAGCGAGTCGTTGTCCGACGGCGGCGTGGCGGAGAAGAAAGAAGAAGCGAAGGCGGCGAAGGCGCCGGGGAAGGTGGGGAAGAGGACGGACTTGAAGAAGATTATGATACTGGGCGCCGGGCCGATTGTGATCGGGCAAGCTTGCGAGTTCGATTACTCGGGGACTCAAGCTTGTAAAGCTTTGAAGGAGGAAGGATACGAGGTCGTTTTGATCAATTCCAACCCGGCTACGATCATGACCGACCCGGATTTGGCCGATAGGACCTACATTACGCCGATGACTCCGGAATTGGTGGAGCAGGTGCTTGAAAAGGAGCGGCCGGACGCTCTTTTGCCGACCATGGGAGGCCAGACGGCGTTGAACCTGGCGGTGGCGTTGGCCGAAAGCGGTGTGCTGGAGAAGTATGGTGTGGAGTTGATTGGAGCTAAGCTGGAGGCCATTAAGAAAGCTGAGGATAGAGAGCTGTTCAAGCAGGCGATGAAGAACATTGGGGTTGAAACGCCGCCGTCTGGAATTGCCAACACCTTGGAGGAATGTGTGGAAATTGCGAAAGAGATTGGTGAGTTTCCCTTGATCATTAGGCCAGCTTTTACGTTGGGAGGGACGGGAGGTGGTATTGCATATAATAAGGAGGAATTTGAGACTATTTGTAAGGCCGGGCTGGCGGCGAGTTTGACCTCTCAGGTTTTGGTGGAGAAGTCACTGTTAGGGTGGAAGGAATATGAGCTTGAGGTGATGAGGGACTTGGCAGATAATGTTGTTATTATTTGCTCCATTGAGAATATGGACCCGATGGGGGTTCATACGGGAGACTCTATCACCGTGGCGCCTGCTCAGACTTTAACTGATAAGGAGTATCAGCGGCTGAGGGACTATTCGATAGCTATAATAAGAGAGATTGGAGTGGAATGTGGTGGTTCTAATGTGCAGTTTGCTGTGAATCCTCAAGACGGGGAGGTTATGGTGATTGAGATGAATCCAAGGGTGTCACGGTCCTCGGCTCTGGCATCCAAAGCAACTGGTTTTCCGATAGCGAAAATGGCTGCTAGGTTGTCAGTTGGATATTCCTTGGATCAAATTCCCAATGACATTACAAAGAAGACACCGGCTAGTTTTGAGCCTTCCATAGACTATGTGGTAACAAAGATTCCCCGGTTTGCTTTTGAAAAGTTTCCTGGTTCAGAGCCAATACTGACGACCCAGATGAAATCTGTCGGTGAGTCAATGGCACTAGGGCGTACATTTCAAGAATCCTTTCAGAAAGCAGTTCGGTCTTTGGAATGTGGTTTCTCTGGATGGGGCTGTGGGAAAATTAAGGAACTAGACTGGGATTGGGAGCAATTGAAATATAGTCTCCGAGTCCCTAACCCAGATCGTATCCATGCTGTGTATGCTGCAATGAAGAAGGGAATGAAGATAGATGAAATTCATGAGCTGAGTTTCATTGACAAATGGTTCCTCACACAGCTTAAAGAATTGGTGGATGTAGAGCAATTCCTCCTGGCTAGAAACTTGTCTGATCTAACAAAGGATGACTTCTATGAAGTGAAAAGAAGAGGCTTTAGTGACAAACAAATAGCTTTTGCCACTAAATCCTCTGAAAATGAAGTGCGGTTGAAGAGATTATCATTGGGTGTCACTCCAGCATATAAGCGGGTAGATACGTGTGCCGCAGAGTTTGAGGCCAATACTCCTTATATGTACTCGTCTTATGACTTTGAGTGTGAATCCGCTCCCACCCAAAAAAAGAAGGTTTTGATTTTAGGTGGAGGACCAAATCGAATTGGTCAGGGTATTGAATTTGACTACTGCTGTTGCCATACATCCTTTGCCCTTCAGAAAGCAGGATATGAGACAATCATGATGAATTCAAATCCTGAAACAGTATCTACTGATTATGACACAAGTGACCGTTTATATTTTGAGCCCCTGACTGTTGAAGATGTGCTGAACATAATTGATTTGGAAAGACCTGATGGGATCATTGTGCAGTTTGGAGGTCAAACACCTCTGAAGCTAGCACTTCCCCTCCAGCGGTATTTAGATGAAAACAAGCCTAGATGTGCAAGTGGTGCTGGACATGTTCGCATTTGGGGTACCACACCTGACTCCATAGATGCTGCTGAGGACAGGGAAAAGTTCAATGCAATTCTGAACGAGTTAAAGATTGAACAGCCAAAAGGAGGAATTGCCAAGAGTGAAGCAGATGCTCTTGCCATTGCCACAAACATTGGCTACCCTGTTGTTGTCCGACCCTCCTATGTATTGGGCGGCCGTGCCATGGAGATAGTATATAGTGATGAAAAACTTGTGACCTACCTAGAAACTGCTGTTGAGGTGGACCCGGAACGTCCTGTATTGATTGACAGGTATCTATCCGATGCCATTGAGATTGATGTTGATGCACTTGCTGACTCTCATGGGAATGTCGTCATTGGTGGGATAATGGAGCACATCGAGCAGGCTGGGGTCCATTCTGGTGATTCTGCTTGCTCGATTCCGACAAAAACCATCCCAGAGTCTTGCTTGAATACAATCAGGTCATGGACTATAAAATTGGCTAAGAGGCTGAATGTATGTGGGCTGATGAACTGTCAGTATGCAATTACAATGTCTGGAGATGTTTATTTACTCGAGGCAAATCCTCGTGCTTCCCGTACGGTCCCTTTTGTGTCCAAGGCAATTGGACATCCGTTGGCTAAATATGCTTCCCTGGTTATGTCTGGGAAGTCTCTTCATGATCTTGGTTTCACAAAAGAAGTCATTCCTGCACATATGTCAGTGAAAGAAGCTGTTCTTCCATTTGAGAAGTTCCAAGGTTGTGATGTATTGCTTGGGCCTGAGATGCGTAGCACTGGTGAGGTGATGGGTATTGATTTTGAGTTTCCAATTGCATTTGCCAAGGCTCAGATTGCTGCTGGGCAGGCGCTACCACTTTCAGGCACTGTGTTTTTGAGCTTAAATGACTTGACCAAACCCCATCTTGAGAGAATTGCAAAGGCATTTTTAGGCCTTGGATTTAAGATTGTTTCAACTGCAGGAACAGCTCATGTTCTTGAACTAGCGAAAATTCCGGTGGAACGAGTGCTGAAGCTACATGAGGGGAGGCCTAATGCTGGTGACACGGTTGCTAATGGGCAAATCCAGTTGATGGTGATTACAAGTTCTGGTGATGCACTCGATCAGATTGATGGACGGCAGCTAAGGAGGACGGCCCTTGCTTACAAGATTCCAGTGATAACAACAGTTGCTGGAGCTTTGGCAACTGCAGAAGCAATAAAGAGCTTAAAGACCAGCTCCATTAAGATGATTGCACTTCAGGACTTCTTTGATGACGAGAATAAAGCTGTCAGTGATAAAAAGTTGCAGTCTTCCTCTGTCTCTCTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000050 GO:0003674 GO:0003824 GO:0004087 GO:0004088 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005951 GO:0006082 GO:0006520 GO:0006525 GO:0006526 GO:0006807 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009064 GO:0009084 GO:0009267 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009987 GO:0009991 GO:0016020 GO:0016036 GO:0016053 GO:0016874 GO:0016879 GO:0016884 GO:0019627 GO:0019752 GO:0031667 GO:0031668 GO:0031669 GO:0032991 GO:0033554 GO:0034641 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0050896 GO:0051716 GO:0071496 GO:0071704 GO:0071941 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1902494

KEGG Pathways

Metabolic & Signaling

ko00240 ko00250 ko01100 map00240 map00250 map01100

Pfam Domains

Protein Families

CPSase_L_D2 CPSase_L_D3 MGS

Protein Analysis

1196

Amino Acids

130.8

Weight (kDa)

5.64

Isoelectric Point (pI)

38.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CPSase_L_D1	PF25596	98 - 215	5.6e-49	Carbamoyl phosphate synthase preATP-grasp domain
CPSase_L_D2	PF02786	219 - 426	1.7e-72	Carbamoyl-phosphate synthase L chain, ATP binding domain
CPSase_L_D3	PF02787	518 - 596	9.1e-27	Carbamoyl-phosphate synthetase large chain, oligomerisation domain
CPSase_L_D1	PF25596	648 - 773	9e-46	Carbamoyl phosphate synthase preATP-grasp domain
CPSase_L_D2	PF02786	777 - 980	4.9e-38	Carbamoyl-phosphate synthase L chain, ATP binding domain
ATP-grasp	PF02222	785 - 951	7.3e-06	ATP-grasp domain
Dala_Dala_lig_C	PF07478	804 - 949	3e-07	D-ala D-ala ligase C-terminus
MGS	PF02142	1063 - 1148	6.3e-19	MGS-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003802)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G29900
fragaria_vesca	FvH4_1g25660 FvH4_1g25660
malus_domestica	MD03G1111400.v1.1 MD11G1125100.v1.1 MD12G1166200.v1.1
prunus_persica	Prupe.6G093800_v2.0.a1
pyrus_communis	pycom11g10560 pycom11g10570
rosa_chinensis	RchiOBHm_Chr2g0124141 RchiOBHm_Chr7g0219181
rosa_laevigata	RLG00000002304 RLG00000018786
rosa_multiflora	Rmu_co8484899.1_g000001 Rmu_sc0003840.1_g000003 Rmu_sc0008812.1_g000002 Rmu_sc0009785.1_g000029
rosa_roxburghii	Rroxscaffold_2G00120100 Rroxscaffold_3G00240170
rosa_rugosa	Rorug02G0248800 Rorug07G0182900 Rorug07G0183000 Rorug07G0183000
rosa_samantha	Rh2AG307800 Rh2BG316800 Rh2CG294600 Rh2DG331600 Rh2DG331800 Rh7AG326600 Rh7BG316400 Rh7CG343900 Rh7DG321900
rosa_wichuraiana	Rw2G024810 Rw7G027510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	490
Acc36I	ACCTGC	1 cut(s)	490
Acc65I	GGTACC	1 cut(s)	2297
AccB1I	GGYRCC	5 cut(s)	122, 262, 314, 1027, 2297
AccB7I	CCANNNNNTGG	1 cut(s)	3352
AccBSI	CCGCTC	2 cut(s)	517, 1934
AccIII	TCCGGA	1 cut(s)	484
AclWI	GGATC	2 cut(s)	1281, 2189
AcoI	YGGCCR	5 cut(s)	152, 453, 518, 579, 2473
AcsI	RAATTY	6 cut(s)	836, 1278, 1626, 2000, 2068, 3270
AcuI	CTGAAG	4 cut(s)	2021, 2234, 3311, 3503
AcyI	GRCGYC	4 cut(s)	123, 263, 315, 1028
AfaI	GTAC	4 cut(s)	1441, 1904, 2299, 2860
AflIII	ACRYGT	2 cut(s)	1865, 2281
AjnI	CCWGG	4 cut(s)	564, 1375, 1701, 2913
Alw21I	GWGCWC	1 cut(s)	2655
Alw26I	GTCTC	6 cut(s)	836, 1005, 1559, 2048, 2937, 3586
AlwI	GGATC	2 cut(s)	1281, 2189
AlwNI	CAGNNNCTG	3 cut(s)	1066, 3146, 3452
Ama87I	CYCGRG	2 cut(s)	358, 2834
Aor13HI	TCCGGA	1 cut(s)	484
ApeKI	GCWGC	6 cut(s)	37, 1244, 1595, 2321, 3116, 3400
ApoI	RAATTY	6 cut(s)	836, 1278, 1626, 2000, 2068, 3270
ArsI	GACNNNNNNTTYG	2 cut(s)	13, 45
Asp700I	GAANNNNTTC	5 cut(s)	92, 1371, 1455, 2964, 2994
Asp718I	GGTACC	1 cut(s)	2297
AspLEI	GCGC	5 cut(s)	125, 265, 317, 1030, 3130
AsuC2I	CCSGG	7 cut(s)	267, 319, 425, 446, 859, 1354, 2546
AsuNHI	GCTAGC	1 cut(s)	2218
AvaI	CYCGRG	2 cut(s)	358, 2834
AvaII	GGWCC	7 cut(s)	462, 988, 1194, 1973, 2542, 2671, 2863
BanI	GGYRCC	5 cut(s)	122, 262, 314, 1027, 2297
BanII	GRGCYC	1 cut(s)	2130
BauI	CACGAG	1 cut(s)	2847
BbsI	GAAGAC	2 cut(s)	1307, 3564
Bbv12I	GWGCWC	1 cut(s)	2655
BbvCI	CCTCAGC	3 cut(s)	651, 1067, 2325
BbvI	GCAGC	6 cut(s)	49, 1231, 1582, 2308, 3103, 3412
BccI	CCATC	8 cut(s)	988, 1491, 2171, 2720, 3061, 3189, 3346, 3386
BceAI	ACGGC	6 cut(s)	235, 570, 691, 2460, 3413, 3429
BcgI	CGANNNNNNTGC	2 cut(s)	152, 186
BciT130I	CCWGG	4 cut(s)	566, 1377, 1703, 2915
BciVI	GTATCC	2 cut(s)	389, 1589
BclI	TGATCA	2 cut(s)	411, 774
BcnI	CCSGG	7 cut(s)	267, 319, 425, 446, 859, 1354, 2546
BcoDI	GTCTC	6 cut(s)	836, 1005, 1559, 2048, 2937, 3586
BfaI	CTAG	9 cut(s)	1248, 1311, 1433, 1523, 1707, 2219, 2259, 2522, 3263
BfmI	CTRYAG	2 cut(s)	3237, 3468
BfoI	RGCGCY	5 cut(s)	126, 266, 318, 1031, 3131
BfuAI	ACCTGC	1 cut(s)	490
BfuI	GTATCC	2 cut(s)	389, 1589
BglI	GCCNNNNNGGC	1 cut(s)	863
BlpI	GCTNAGC	2 cut(s)	137, 627
Bme18I	GGWCC	7 cut(s)	462, 988, 1194, 1973, 2542, 2671, 2863
BmeT110I	CYCGRG	2 cut(s)	358, 2834
BmrI	ACTGGG	2 cut(s)	320, 1537
BmsI	GCATC	7 cut(s)	1217, 2308, 2398, 2572, 2593, 3036, 3364
BmtI	GCTAGC	1 cut(s)	2222
BmuI	ACTGGG	2 cut(s)	320, 1537
BpiI	GAAGAC	2 cut(s)	1307, 3564
BpmI	CTGGAG	6 cut(s)	617, 653, 1827, 2216, 2838, 3474
Bpu10I	CCTNAGC	4 cut(s)	651, 1067, 2325, 3404
Bpu1102I	GCTNAGC	2 cut(s)	137, 627
BpuEI	CTTGAG	4 cut(s)	351, 950, 1131, 3206
BpuMI	CCSGG	7 cut(s)	267, 319, 425, 446, 859, 1354, 2546
BsaAI	YACGTR	1 cut(s)	1866
BsaBI	GATNNNNATC	1 cut(s)	329
BsaHI	GRCGYC	4 cut(s)	123, 263, 315, 1028
BsaWI	WCCGGW	3 cut(s)	144, 484, 3274
BsaXI	ACNNNNNCTCC	2 cut(s)	2214, 2244
Bse118I	RCCGGY	1 cut(s)	1306
Bse1I	ACTGG	6 cut(s)	315, 1225, 1532, 3061, 3346, 3434
Bse3DI	GCAATG	3 cut(s)	7, 1605, 2418
Bse8I	GATNNNNATC	1 cut(s)	329
BseAI	TCCGGA	1 cut(s)	484
BseBI	CCWGG	4 cut(s)	566, 1377, 1703, 2915
BseGI	GGATG	7 cut(s)	1208, 1502, 1690, 1738, 2024, 2712, 2890
BseJI	GATNNNNATC	1 cut(s)	329
BseMI	GCAATG	3 cut(s)	7, 1605, 2418
BseNI	ACTGG	6 cut(s)	315, 1225, 1532, 3061, 3346, 3434
BseRI	GAGGAG	1 cut(s)	58
BseX3I	CGGCCG	2 cut(s)	518, 2473
BseXI	GCAGC	6 cut(s)	49, 1231, 1582, 2308, 3103, 3412
BseYI	CCCAGC	2 cut(s)	2666, 3119
BsgI	GTGCAG	3 cut(s)	1148, 2210, 2955
Bsh1285I	CGRYCG	2 cut(s)	521, 2476
BshNI	GGYRCC	5 cut(s)	122, 262, 314, 1027, 2297
BsiEI	CGRYCG	2 cut(s)	521, 2476
BsiHKAI	GWGCWC	1 cut(s)	2655
BsiHKCI	CYCGRG	2 cut(s)	358, 2834
BsiWI	CGTACG	1 cut(s)	2858
BslFI	GGGAC	6 cut(s)	376, 817, 956, 1085, 1547, 2849
BsmAI	GTCTC	6 cut(s)	836, 1005, 1559, 2048, 2937, 3586
BsmFI	GGGAC	6 cut(s)	376, 817, 956, 1085, 1547, 2849
BsoBI	CYCGRG	2 cut(s)	358, 2834
Bsp1286I	GDGCHC	2 cut(s)	2130, 2655
Bsp13I	TCCGGA	1 cut(s)	484
Bsp1720I	GCTNAGC	2 cut(s)	137, 627
Bsp19I	CCATGG	2 cut(s)	540, 2481
BspEI	TCCGGA	1 cut(s)	484
BspHI	TCATGA	4 cut(s)	435, 1630, 2061, 2938
BspMAI	CTGCAG	2 cut(s)	3241, 3472
BspMI	ACCTGC	1 cut(s)	490
BspOI	GCTAGC	1 cut(s)	2222
BspPI	GGATC	2 cut(s)	1281, 2189
BspT107I	GGYRCC	5 cut(s)	122, 262, 314, 1027, 2297
BsrBI	CCGCTC	2 cut(s)	517, 1934
BsrDI	GCAATG	3 cut(s)	7, 1605, 2418
BsrFI	RCCGGY	1 cut(s)	1306
BsrI	ACTGG	6 cut(s)	315, 1225, 1532, 3061, 3346, 3434
BssAI	RCCGGY	1 cut(s)	1306
BssNI	GRCGYC	4 cut(s)	123, 263, 315, 1028
BssSI	CACGAG	1 cut(s)	2847
BssT1I	CCWWGG	9 cut(s)	540, 726, 1181, 1269, 2481, 2877, 3016, 3102, 3214
Bst2BI	CACGAG	1 cut(s)	2847
Bst2UI	CCWGG	4 cut(s)	566, 1377, 1703, 2915
Bst6I	CTCTTC	4 cut(s)	275, 1750, 1814, 2940
BstACI	GRCGYC	4 cut(s)	123, 263, 315, 1028
BstAPI	GCANNNNNTGC	1 cut(s)	2273
BstBAI	YACGTR	1 cut(s)	1866
BstDSI	CCRYGG	3 cut(s)	540, 1023, 2481
BstF5I	GGATG	7 cut(s)	1208, 1502, 1690, 1738, 2024, 2712, 2890
BstH2I	RGCGCY	5 cut(s)	126, 266, 318, 1031, 3131
BstHHI	GCGC	5 cut(s)	125, 265, 317, 1030, 3130
BstMAI	GTCTC	6 cut(s)	836, 1005, 1559, 2048, 2937, 3586
BstMCI	CGRYCG	2 cut(s)	521, 2476
BstNI	CCWGG	4 cut(s)	566, 1377, 1703, 2915
BstNSI	RCATGY	1 cut(s)	2285
BstSFI	CTRYAG	2 cut(s)	3237, 3468
BstV1I	GCAGC	6 cut(s)	49, 1231, 1582, 2308, 3103, 3412
BstV2I	GAAGAC	2 cut(s)	1307, 3564
BstXI	CCANNNNNNTGG	1 cut(s)	727
BstZI	CGGCCG	2 cut(s)	518, 2473
BsuI	GTATCC	2 cut(s)	389, 1589
BtgI	CCRYGG	3 cut(s)	540, 1023, 2481
BtgZI	GCGATG	1 cut(s)	692
BtsCI	GGATG	7 cut(s)	1208, 1502, 1690, 1738, 2024, 2712, 2890
BtsIMutI	CAGTG	6 cut(s)	902, 2988, 3054, 3144, 3441, 3559
BveI	ACCTGC	1 cut(s)	490
CaiI	CAGNNNCTG	3 cut(s)	1066, 3146, 3452
CciI	TCATGA	4 cut(s)	435, 1630, 2061, 2938
CfoI	GCGC	5 cut(s)	125, 265, 317, 1030, 3130
Cfr10I	RCCGGY	1 cut(s)	1306
CseI	GACGC	1 cut(s)	533
Csp6I	GTAC	4 cut(s)	1440, 1903, 2298, 2859
CspCI	CAANNNNNGTGG	2 cut(s)	2413, 2448
CviQI	GTAC	4 cut(s)	1440, 1903, 2298, 2859
DinI	GGCGCC	4 cut(s)	124, 264, 316, 1029
EaeI	YGGCCR	5 cut(s)	152, 453, 518, 579, 2473
EagI	CGGCCG	2 cut(s)	518, 2473
Eam1104I	CTCTTC	4 cut(s)	275, 1750, 1814, 2940
EarI	CTCTTC	4 cut(s)	275, 1750, 1814, 2940
EciI	GGCGGA	1 cut(s)	245
EclXI	CGGCCG	2 cut(s)	518, 2473
Eco130I	CCWWGG	9 cut(s)	540, 726, 1181, 1269, 2481, 2877, 3016, 3102, 3214
Eco147I	AGGCCT	2 cut(s)	3213, 3310
Eco24I	GRGCYC	1 cut(s)	2130
Eco47I	GGWCC	7 cut(s)	462, 988, 1194, 1973, 2542, 2671, 2863
Eco52I	CGGCCG	2 cut(s)	518, 2473
Eco57I	CTGAAG	4 cut(s)	2021, 2234, 3311, 3503
Eco88I	CYCGRG	2 cut(s)	358, 2834
EcoO109I	RGGNCCY	1 cut(s)	462
EcoRI	GAATTC	1 cut(s)	2068
EcoRII	CCWGG	4 cut(s)	564, 1375, 1701, 2913
EcoT14I	CCWWGG	9 cut(s)	540, 726, 1181, 1269, 2481, 2877, 3016, 3102, 3214
EcoT38I	GRGCYC	1 cut(s)	2130
EgeI	GGCGCC	4 cut(s)	124, 264, 316, 1029
EheI	GGCGCC	4 cut(s)	124, 264, 316, 1029
ErhI	CCWWGG	9 cut(s)	540, 726, 1181, 1269, 2481, 2877, 3016, 3102, 3214
FalI	AAGNNNNNCTT	4 cut(s)	1721, 1753, 2982, 3014
FaqI	GGGAC	6 cut(s)	376, 817, 956, 1085, 1547, 2849
FauI	CCCGC	1 cut(s)	1848
FauNDI	CATATG	1 cut(s)	2976
FbaI	TGATCA	2 cut(s)	411, 774
FokI	GGATG	7 cut(s)	1195, 1509, 1697, 1745, 2011, 2699, 2877
FriOI	GRGCYC	1 cut(s)	2130
FspBI	CTAG	9 cut(s)	1248, 1311, 1433, 1523, 1707, 2219, 2259, 2522, 3263
GlaI	GCGC	5 cut(s)	124, 264, 316, 1029, 3129
GsaI	CCCAGC	2 cut(s)	2670, 3123
GsuI	CTGGAG	6 cut(s)	617, 653, 1827, 2216, 2838, 3474
HaeII	RGCGCY	5 cut(s)	126, 266, 318, 1031, 3131
HgaI	GACGC	1 cut(s)	533
HhaI	GCGC	5 cut(s)	125, 265, 317, 1030, 3130
Hin1I	GRCGYC	4 cut(s)	123, 263, 315, 1028
Hin6I	GCGC	5 cut(s)	123, 263, 315, 1028, 3128
HinP1I	GCGC	5 cut(s)	123, 263, 315, 1028, 3128
HincII	GTYRAC	1 cut(s)	190
HindII	GTYRAC	1 cut(s)	190
HindIII	AAGCTT	3 cut(s)	339, 369, 378
Hpy166II	GTNNAC	2 cut(s)	190, 2542
Hpy8I	GTNNAC	2 cut(s)	190, 2542
Hpy99I	CGWCG	3 cut(s)	82, 136, 221
HpyCH4IV	ACGT	3 cut(s)	794, 1865, 2551
HpySE526I	ACGT	3 cut(s)	794, 1865, 2551
Hsp92I	GRCGYC	4 cut(s)	123, 263, 315, 1028
HspAI	GCGC	5 cut(s)	123, 263, 315, 1028, 3128
KasI	GGCGCC	4 cut(s)	122, 262, 314, 1027
Kpn2I	TCCGGA	1 cut(s)	484
KpnI	GGTACC	1 cut(s)	2301
Ksp22I	TGATCA	2 cut(s)	411, 774
Lsp1109I	GCAGC	6 cut(s)	49, 1231, 1582, 2308, 3103, 3412
LweI	GCATC	7 cut(s)	1217, 2308, 2398, 2572, 2593, 3036, 3364
MaeI	CTAG	9 cut(s)	1248, 1311, 1433, 1523, 1707, 2219, 2259, 2522, 3263
MaeII	ACGT	3 cut(s)	794, 1865, 2551
MaeIII	GTNAC	8 cut(s)	900, 1188, 1339, 1766, 1837, 2108, 2512, 3320
MbiI	CCGCTC	2 cut(s)	517, 1934
MfeI	CAATTG	3 cut(s)	1541, 2883, 3090
MhlI	GDGCHC	2 cut(s)	2130, 2655
Mly113I	GGCGCC	4 cut(s)	123, 263, 315, 1028
MmeI	TCCRAC	5 cut(s)	240, 444, 1240, 2476, 2726
MroI	TCCGGA	1 cut(s)	484
MroXI	GAANNNNTTC	5 cut(s)	92, 1371, 1455, 2964, 2994
MseI	TTAA	9 cut(s)	642, 1043, 1515, 1671, 2363, 3161, 3222, 3488, 3504
MslI	CAYNNNNRTG	3 cut(s)	2625, 2679, 3506
MspA1I	CMGCKG	2 cut(s)	1063, 2236
MunI	CAATTG	3 cut(s)	1541, 2883, 3090
MvaI	CCWGG	4 cut(s)	566, 1377, 1703, 2915
NarI	GGCGCC	4 cut(s)	123, 263, 315, 1028
NciI	CCSGG	7 cut(s)	267, 319, 425, 446, 859, 1354, 2546
NcoI	CCATGG	2 cut(s)	540, 2481
NdeI	CATATG	1 cut(s)	2976
NheI	GCTAGC	1 cut(s)	2218
NmeAIII	GCCGAG	3 cut(s)	99, 209, 1178
NmuCI	GTSAC	7 cut(s)	900, 1188, 1766, 1837, 2108, 2512, 3320
NspI	RCATGY	1 cut(s)	2285
PaeR7I	CTCGAG	1 cut(s)	2834
PagI	TCATGA	4 cut(s)	435, 1630, 2061, 2938
PaqCI	CACCTGC	1 cut(s)	490
PceI	AGGCCT	2 cut(s)	3213, 3310
PciI	ACATGT	1 cut(s)	2281
PcsI	WCGNNNNNNNCGW	2 cut(s)	83, 2854
PdmI	GAANNNNTTC	5 cut(s)	92, 1371, 1455, 2964, 2994
PfeI	GAWTC	8 cut(s)	1141, 1177, 1348, 1451, 1928, 2684, 2698, 3430
Pfl23II	CGTACG	1 cut(s)	2858
PflFI	GACNNNGTC	1 cut(s)	25
PflMI	CCANNNNNTGG	1 cut(s)	3352
PluTI	GGCGCC	4 cut(s)	126, 266, 318, 1031
Ppu21I	YACGTR	1 cut(s)	1866
PpuMI	RGGWCCY	1 cut(s)	462
PscI	ACATGT	1 cut(s)	2281
Psp5II	RGGWCCY	1 cut(s)	462
Psp6I	CCWGG	4 cut(s)	564, 1375, 1701, 2913
PspFI	CCCAGC	2 cut(s)	2666, 3119
PspGI	CCWGG	4 cut(s)	564, 1375, 1701, 2913
PspLI	CGTACG	1 cut(s)	2858
PspPPI	RGGWCCY	1 cut(s)	462
PspXI	VCTCGAGB	1 cut(s)	2834
PstI	CTGCAG	2 cut(s)	3241, 3472
PstNI	CAGNNNCTG	3 cut(s)	1066, 3146, 3452
PsyI	GACNNNGTC	1 cut(s)	25
RsaI	GTAC	4 cut(s)	1441, 1904, 2299, 2860
RsaNI	GTAC	4 cut(s)	1440, 1903, 2298, 2859
RseI	CAYNNNNRTG	3 cut(s)	2625, 2679, 3506
SaqAI	TTAA	9 cut(s)	642, 1043, 1515, 1671, 2363, 3161, 3222, 3488, 3504
SduI	GDGCHC	2 cut(s)	2130, 2655
SfaNI	GCATC	7 cut(s)	1217, 2308, 2398, 2572, 2593, 3036, 3364
SfcI	CTRYAG	2 cut(s)	3237, 3468
SfoI	GGCGCC	4 cut(s)	124, 264, 316, 1029
Sfr274I	CTCGAG	1 cut(s)	2834
SinI	GGWCC	7 cut(s)	462, 988, 1194, 1973, 2542, 2671, 2863
SlaI	CTCGAG	1 cut(s)	2834
SmiMI	CAYNNNNRTG	3 cut(s)	2625, 2679, 3506
SmlI	CTYRAG	5 cut(s)	366, 929, 1146, 2834, 3185
SmoI	CTYRAG	5 cut(s)	366, 929, 1146, 2834, 3185
SseBI	AGGCCT	2 cut(s)	3213, 3310
SspDI	GGCGCC	4 cut(s)	122, 262, 314, 1027
SspMI	CTAG	9 cut(s)	1248, 1311, 1433, 1523, 1707, 2219, 2259, 2522, 3263
StuI	AGGCCT	2 cut(s)	3213, 3310
StyI	CCWWGG	9 cut(s)	540, 726, 1181, 1269, 2481, 2877, 3016, 3102, 3214
TaiI	ACGT	3 cut(s)	797, 1868, 2554
TaqI	TCGA	8 cut(s)	80, 351, 1079, 1981, 2658, 2696, 2835, 3381
TaqII	GACCGA	2 cut(s)	455, 1461
TatI	WGTACW	1 cut(s)	1902
TfiI	GAWTC	8 cut(s)	1141, 1177, 1348, 1451, 1928, 2684, 2698, 3430
Tru1I	TTAA	9 cut(s)	642, 1043, 1515, 1671, 2363, 3161, 3222, 3488, 3504
Tru9I	TTAA	9 cut(s)	642, 1043, 1515, 1671, 2363, 3161, 3222, 3488, 3504
TscAI	CASTG	6 cut(s)	909, 2988, 3061, 3151, 3441, 3559
TseFI	GTSAC	7 cut(s)	900, 1188, 1766, 1837, 2108, 2512, 3320
TseI	GCWGC	6 cut(s)	37, 1244, 1595, 2321, 3116, 3400
Tsp45I	GTSAC	7 cut(s)	900, 1188, 1766, 1837, 2108, 2512, 3320
TspGWI	ACGGA	2 cut(s)	302, 2883
TspRI	CASTG	6 cut(s)	909, 2988, 3061, 3151, 3441, 3559
Tth111I	GACNNNGTC	1 cut(s)	25
Van91I	CCANNNNNTGG	1 cut(s)	3352
VpaK11BI	GGWCC	7 cut(s)	462, 988, 1194, 1973, 2542, 2671, 2863
XapI	RAATTY	6 cut(s)	836, 1278, 1626, 2000, 2068, 3270
XceI	RCATGY	1 cut(s)	2285
XcmI	CCANNNNNNNNNTGG	2 cut(s)	982, 1333
XhoI	CTCGAG	1 cut(s)	2834
XmnI	GAANNNNTTC	5 cut(s)	92, 1371, 1455, 2964, 2994
XspI	CTAG	9 cut(s)	1248, 1311, 1433, 1523, 1707, 2219, 2259, 2522, 3263

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.