RchiOBHm_Chr1g0337141

acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0001590
Physical Location & Seq
Reverse (-)
28807703 .. 28818277
10575 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2922 bp
ATGTCGACCTCATTATCACCTGCACGACGCCGTTTGGTTACGAATTGGACTCCAATTGGCTGGAAAAGAATTCCAATCGTAGCTTTCTGTGGTTTTCCATGGGTGGTGGAGTCTGTGACAGTGAGTGATGGAGAGCAGCGTAAGAGTGAGAAAGAGAGGCTGTTCTGTTATGTAATGTACACTTCGGGCTCGACAGGGAAGCCGAAAGGTGTCTGTGGAACTGAACAAGGTCTTTGGAATCGGTTTCGGTGGATGCAAGAATTGTATCCATTGAATGGAGAGGAGGTTTTGTTGTTCAAGACAGCAATAAGCTTTGTTGATCACTTGCAAGAATTTCTTAGTGCTATTCTTACTGGTTCTACTTTGGTCATACCTCCTTTCAATCAACTTAAACAGAATGTGTTCTCCATTCTCGATTTTCTACAGGCTTATTCTATCAATAGACTTACTGCTGTTCCATCACTGATGAGAGTAATCCTTCCAGCTTTGCAAGGTCGAGACTACCAGCAGCTTCTAAGTTCACTGAATTTGTTAGTGCTAAGCGGTGAAGTTTTGCCTTTATCCTTGTGGGATATGCTGTCAAAGAGATTACCAAGGACCTCTATTTTGAATCTGTATGGGAGCACGGAGGTGTCAGGTGATTGTACATATTTTGACTGCAAGAGGTTGCCAATGATTTTGGAGACAGAGACGCTAACAAGTGTTCCAATTGGTATACCTATAGCTGGTTGTGATGTTGTGCTTGTTAATGATGATGATGTACTCAATGAAGGAGAAATTTTTGTTGCTGGTCCCTGCAATGCTTCTGGATACTATTCTGATTCTACTTTTGCTCCTTTGAACACTGTAAAATTACCTCCAGATTCTGTTTGTTCCAGTTCTGTAAATGGACACGAAAGTCAATCATATTTTCAAACTGGTGATTTCGCCAAACAACTTCATAGCGGTGACTTGGTTTTCTTGGGAAGGAAGAACCGCACTATTAAGCTCAATGGGCAGCGTATTGCTTTAGAAGAAATTGTACATTCATTGGTGGAACATCCAGATGTAACTGATGCTGCTGTTGTTTTTCGTAATGGTCAAGGGGAACTTATGCAACTTGTAGCATTTATAATGTTAAAGGAGGGAAGACCTGATGAAATATTCAGATCTTCCATCAAAAGTTGGATGGTTGACAAACTTCCACTGGCAATGATTCCTGGCCACTTTGTTATAATGGAATCATTTCCTGTGTCTACTAGTGGAAAAGTTGACTATGCTTTGTTAGCAGATTCAGTATTTGTGGCAAAACATATCCAAGATGAGCTTGGTCATATTGGGAGAAGCAATCTACTGCAAGTAATTAAAAAGGCCTTTAGTCGTGTTTTAATGATGACTGAAGAGGTTTCCGATGATGATGATTTCTTTATGATTGGTGGAGATTCTATTGCTGCTGCACATCTTGCTAACAATATAGGAGTTGATATGAGATTGATATATAGCTTTCCAACTCCATCTAAGCTTTCTATGGCTCTTCTAGAGAGAAAAGAACCATTCAGTATGAAAGTTGAAGTGGATTCTGAATCCGAGATGAATCAGGAAAGGGGAAAGAACATGTTTCATGTCCTCTATGACACTCCTAGTGCTGTTAATCTTGAACAGTTGAAAACTCTGCCTAGAAGGAATGAAAATAAAGCAGTTATGTCTAAACGCTTGAAAGCCGATTCAAGTATAAATGTTGCTTCAGGGAGTACTAGTCCAGCAGATGGGTATCCATGGAGTTCTATGCAAAAATGCATGACATGTTCTTTCAGCAGGTGCAACAAAGTTGTGTATGAGGGCAGTTCCAGGGTGAATGATGTTTGTCAAGCGGCTAGTTCTGTGGTTGTCCCAAAAAGTCTAAAAGTGCATTTGGAAGAATTGTGGAAAGTTGACATGGGATCCTGCGTTGACGCATCACCACTTATTGTTTGTAAAGGCCAGGATATCTACTTATTTATTGGATCACACTCACAAAAGTTTGTGTGTGTTAATGCCAGAAGTGGTTCCATCCAATGGGAGTTACAACTTGAAGGACGTGTAGAATGTTCAGCAGCAATTCTTGGTGACTTTTCTCAGGTTGTAGTTGGATGCTACAAAGGGAAGATATATTTCCTGGATTCTTTGAATGGTAACATATGCTGGACTTTCCAAACATCTGGTGAGGTAAAGTCACAGCCGATTATAGACAGTCAAAGACAATTGATCTGGTGTGGGTCATATGACCGTAACTTGTATGTGCTAGATTATAAAAATCATTGCTGTGTTTATCAGCTTCCATGTGGTGGGAGTATCTGTGGGTCTCCTGCAATAGATGAGATGAATAATATACTTTATGTGGCTTCTACTGGTGGCCAAATGACAGCCATTTCAGTAACGTTATATGTTGCTTGGTGGATGGGCATGTTCTTGCATTTAATACAAGTGGATCTATTATTTGGAGGAACTGCTGGGCCTGTATTTGCTGGAGCCTGCATATCTTCTGCTCTTCCCTTTCAGCTACTTGTCTGTTCCAGAGATGGAAACATCTATTCACTCGAACTCGAATCAGGAGGTTTACTCTGGGAATACAATGTTAAGGATCCAATTACTTCATCTGCCTATGTCGATGAGCACCTGAGCTTGGTGTCTGATCAGTCTAACTTTCCCGACCGCAGGGTGGTTTGTGTATGTTCCAGCTCCGGAAGCATGGTTCTGATTCAGGTTAATTTGGATGCCACCGAAGGTGCAAGTCCAATGAATTTCAATGTTGAGGAATTTGCAAGGTTGGAACTCCCAGGAGATGTATTCTCTTCCCCTGTAATGATTGGTGGCAGAATTTTCATTGGTTGTAGGGATGATTATGTGCACTGCATTGCTGTTAAGCCCCAGGTCTCAACAGGAACAATGAGTTGTTCTGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C PP-binding PQQ PQQ_2 PQQ_3

Protein Analysis

973

Amino Acids

107.44

Weight (kDa)

5.35

Isoelectric Point (pI)

42.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 46 - 272 2.4e-39 AMP-binding enzyme
AMP-binding_C PF13193 343 - 416 1.3e-07 AMP-binding enzyme C-terminal domain
PP-binding PF00550 447 - 501 7.4e-06 Phosphopantetheine attachment site
PQQ_2 PF13360 632 - 736 4.7e-08 Outer membrane protein assembly factor BamB
Beta-prop_ACSF4 PF13570 639 - 800 4.1e-57 Beta-alanine-activating enzyme, beta-propeller
Beta-prop_ACSF4 PF13570 822 - 959 2.5e-23 Beta-alanine-activating enzyme, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1112, 1214, 2268
AarI CACCTGC 2 cut(s) 28, 1785
AasI GACNNNNNNGTC 1 cut(s) 897
Acc36I ACCTGC 2 cut(s) 28, 1785
AccB7I CCANNNNNTGG 4 cut(s) 275, 1745, 2034, 2303
AccI GTMKAC 3 cut(s) 5, 715, 1235
AccIII TCCGGA 1 cut(s) 2699
AciI CCGC 5 cut(s) 543, 945, 976, 1850, 2671
AclI AACGTT 1 cut(s) 2396
AclWI GGATC 6 cut(s) 1914, 1927, 1990, 2454, 2594, 2607
AcoI YGGCCR 2 cut(s) 1201, 2371
AcsI RAATTY 7 cut(s) 69, 332, 526, 777, 2758, 2774, 2835
AcuI CTGAAG 2 cut(s) 1398, 1707
AcyI GRCGYC 1 cut(s) 28
AfaI GTAC 5 cut(s) 179, 646, 762, 1023, 1732
AfiI CCNNNNNNNGG 8 cut(s) 275, 725, 1745, 2034, 2303, 2641, 2673, 2677
AflIII ACRYGT 3 cut(s) 1593, 1781, 2056
AhlI ACTAGT 2 cut(s) 1238, 1733
AjiI CACGTC 1 cut(s) 2057
AjnI CCWGG 6 cut(s) 1198, 1826, 1959, 2133, 2794, 2886
AleI CACNNNNGTG 1 cut(s) 629
AloI GAACNNNNNNTCC 2 cut(s) 2694, 2726
Alw21I GWGCWC 3 cut(s) 626, 2634, 2868
Alw26I GTCTC 5 cut(s) 492, 677, 683, 2325, 2896
Alw44I GTGCAC 1 cut(s) 2864
AlwI GGATC 6 cut(s) 1914, 1927, 1990, 2454, 2594, 2607
AlwNI CAGNNNCTG 1 cut(s) 866
Aor13HI TCCGGA 1 cut(s) 2699
AoxI GGCC 5 cut(s) 1201, 1350, 1957, 2371, 2471
ApaLI GTGCAC 1 cut(s) 2864
ApeKI GCWGC 7 cut(s) 136, 508, 997, 1058, 1430, 1433, 2072
ApoI RAATTY 7 cut(s) 69, 332, 526, 777, 2758, 2774, 2835
ArsI GACNNNNNNTTYG 2 cut(s) 589, 621
Asp700I GAANNNNTTC 3 cut(s) 401, 1224, 2023
AspS9I GGNCC 3 cut(s) 597, 791, 2471
AsuHPI GGTGA 9 cut(s) 9, 557, 650, 932, 959, 1843, 1929, 2096, 2192
AvaII GGWCC 2 cut(s) 597, 791
BaeGI GKGCMC 1 cut(s) 2868
BalI TGGCCA 2 cut(s) 1203, 2373
BamHI GGATCC 2 cut(s) 1919, 2599
BanII GRGCYC 1 cut(s) 191
BarI GAAGNNNNNNTAC 2 cut(s) 1005, 1037
BbsI GAAGAC 1 cut(s) 1135
Bbv12I GWGCWC 3 cut(s) 626, 2634, 2868
BbvI GCAGC 7 cut(s) 148, 520, 1009, 1045, 1417, 1420, 2084
BccI CCATC 9 cut(s) 122, 466, 1162, 1163, 1501, 1739, 2036, 2410, 2531
BceAI ACGGC 1 cut(s) 15
BciT130I CCWGG 6 cut(s) 1200, 1828, 1961, 2135, 2796, 2888
BciVI GTATCC 3 cut(s) 276, 803, 1761
BclI TGATCA 2 cut(s) 319, 2650
BcoDI GTCTC 5 cut(s) 492, 677, 683, 2325, 2896
BcuI ACTAGT 2 cut(s) 1238, 1733
BfaI CTAG 7 cut(s) 1239, 1517, 1620, 1656, 1734, 1854, 2261
BfmI CTRYAG 2 cut(s) 422, 720
BfuAI ACCTGC 2 cut(s) 28, 1785
BfuI GTATCC 3 cut(s) 276, 803, 1761
BglII AGATCT 1 cut(s) 1148
BisI GCNGC 8 cut(s) 137, 509, 998, 1059, 1431, 1434, 1851, 2073
BlpI GCTNAGC 1 cut(s) 539
BlsI GCNGC 8 cut(s) 138, 510, 999, 1060, 1432, 1435, 1852, 2074
BmcAI AGTACT 1 cut(s) 1732
Bme1390I CCNGG 6 cut(s) 1200, 1828, 1961, 2135, 2796, 2888
Bme18I GGWCC 2 cut(s) 597, 791
BmgBI CACGTC 1 cut(s) 2057
BmgT120I GGNCC 3 cut(s) 597, 791, 2471
BmiI GGNNCC 5 cut(s) 793, 1921, 2026, 2488, 2601
BmrFI CCNGG 6 cut(s) 1200, 1828, 1961, 2135, 2796, 2888
BmsI GCATC 5 cut(s) 243, 1045, 1943, 2099, 2722
BpiI GAAGAC 1 cut(s) 1135
BplI GAGNNNNNCTC 2 cut(s) 2562, 2594
BpmI CTGGAG 2 cut(s) 843, 2505
Bpu10I CCTNAGC 1 cut(s) 2636
Bpu1102I GCTNAGC 1 cut(s) 539
BsaBI GATNNNNATC 1 cut(s) 1749
BsaHI GRCGYC 1 cut(s) 28
BsaI GGTCTC 2 cut(s) 2325, 2896
BsaJI CCNNGG 6 cut(s) 98, 593, 1754, 1827, 2794, 2886
BsaWI WCCGGW 1 cut(s) 2699
Bsc4I CCNNNNNNNGG 8 cut(s) 275, 725, 1745, 2034, 2303, 2641, 2673, 2677
Bse1I ACTGG 5 cut(s) 358, 876, 922, 1191, 2371
Bse3DI GCAATG 4 cut(s) 805, 1197, 2275, 2871
Bse8I GATNNNNATC 1 cut(s) 1749
BseAI TCCGGA 1 cut(s) 2699
BseBI CCWGG 6 cut(s) 1200, 1828, 1961, 2135, 2796, 2888
BseDI CCNNGG 6 cut(s) 98, 593, 1754, 1827, 2794, 2886
BseGI GGATG 8 cut(s) 258, 1039, 1173, 2028, 2114, 2421, 2737, 2860
BseJI GATNNNNATC 1 cut(s) 1749
BseLI CCNNNNNNNGG 8 cut(s) 275, 725, 1745, 2034, 2303, 2641, 2673, 2677
BseMI GCAATG 4 cut(s) 805, 1197, 2275, 2871
BseMII CTCAG 2 cut(s) 2108, 2627
BseNI ACTGG 5 cut(s) 358, 876, 922, 1191, 2371
BseRI GAGGAG 1 cut(s) 296
BseSI GKGCMC 1 cut(s) 2868
BseXI GCAGC 7 cut(s) 148, 520, 1009, 1045, 1417, 1420, 2084
BseYI CCCAGC 1 cut(s) 2468
BsgI GTGCAG 2 cut(s) 6, 1419
Bsh1285I CGRYCG 1 cut(s) 2671
BshFI GGCC 5 cut(s) 1203, 1352, 1959, 2373, 2473
BsiEI CGRYCG 1 cut(s) 2671
BsiHKAI GWGCWC 3 cut(s) 626, 2634, 2868
BsiSI CCGG 1 cut(s) 2700
BslFI GGGAC 2 cut(s) 777, 1853
BslI CCNNNNNNNGG 8 cut(s) 275, 725, 1745, 2034, 2303, 2641, 2673, 2677
BsmAI GTCTC 5 cut(s) 492, 677, 683, 2325, 2896
BsmBI CGTCTC 1 cut(s) 683
BsmFI GGGAC 2 cut(s) 777, 1853
BsnI GGCC 5 cut(s) 1203, 1352, 1959, 2373, 2473
Bso31I GGTCTC 2 cut(s) 2325, 2896
Bsp1286I GDGCHC 4 cut(s) 191, 626, 2634, 2868
Bsp13I TCCGGA 1 cut(s) 2699
Bsp1407I TGTACA 3 cut(s) 177, 644, 1021
Bsp143I GATC 8 cut(s) 319, 1148, 1919, 1982, 2223, 2446, 2599, 2650
Bsp1720I GCTNAGC 1 cut(s) 539
Bsp19I CCATGG 2 cut(s) 98, 1754
BspACI CCGC 5 cut(s) 543, 945, 976, 1850, 2671
BspANI GGCC 5 cut(s) 1203, 1352, 1959, 2373, 2473
BspCNI CTCAG 2 cut(s) 2107, 2628
BspEI TCCGGA 1 cut(s) 2699
BspLI GGNNCC 5 cut(s) 793, 1921, 2026, 2488, 2601
BspMI ACCTGC 2 cut(s) 28, 1785
BspPI GGATC 6 cut(s) 1914, 1927, 1990, 2454, 2594, 2607
BspQI GCTCTTC 2 cut(s) 1518, 2511
BspTNI GGTCTC 2 cut(s) 2325, 2896
BsrDI GCAATG 4 cut(s) 805, 1197, 2275, 2871
BsrGI TGTACA 3 cut(s) 177, 644, 1021
BsrI ACTGG 5 cut(s) 358, 876, 922, 1191, 2371
BssECI CCNNGG 6 cut(s) 98, 593, 1754, 1827, 2794, 2886
BssMI GATC 8 cut(s) 319, 1148, 1919, 1982, 2223, 2446, 2599, 2650
BssNAI GTATAC 1 cut(s) 716
BssNI GRCGYC 1 cut(s) 28
BssT1I CCWWGG 3 cut(s) 98, 593, 1754
Bst1107I GTATAC 1 cut(s) 716
Bst2UI CCWGG 6 cut(s) 1200, 1828, 1961, 2135, 2796, 2888
Bst4CI ACNGT 5 cut(s) 121, 847, 1641, 2210, 2246
Bst6I CTCTTC 4 cut(s) 1374, 1518, 2511, 2815
BstACI GRCGYC 1 cut(s) 28
BstAPI GCANNNNNTGC 1 cut(s) 1442
BstAUI TGTACA 3 cut(s) 177, 644, 1021
BstC8I GCNNGC 1 cut(s) 2491
BstDEI CTNAG 6 cut(s) 338, 515, 539, 1497, 2094, 2636
BstDSI CCRYGG 2 cut(s) 98, 1754
BstF5I GGATG 8 cut(s) 258, 1039, 1173, 2028, 2114, 2421, 2737, 2860
BstKTI GATC 8 cut(s) 322, 1151, 1922, 1985, 2226, 2449, 2602, 2653
BstMAI GTCTC 5 cut(s) 492, 677, 683, 2325, 2896
BstMBI GATC 8 cut(s) 319, 1148, 1919, 1982, 2223, 2446, 2599, 2650
BstMCI CGRYCG 1 cut(s) 2671
BstMWI GCNNNNNNNGC 4 cut(s) 994, 1265, 1442, 2703
BstNI CCWGG 6 cut(s) 1200, 1828, 1961, 2135, 2796, 2888
BstNSI RCATGY 3 cut(s) 1597, 1785, 2425
BstSCI CCNGG 6 cut(s) 1198, 1826, 1959, 2133, 2794, 2886
BstSFI CTRYAG 2 cut(s) 422, 720
BstSLI GKGCMC 1 cut(s) 2868
BstV1I GCAGC 7 cut(s) 148, 520, 1009, 1045, 1417, 1420, 2084
BstV2I GAAGAC 1 cut(s) 1135
BstX2I RGATCY 4 cut(s) 1148, 1919, 2446, 2599
BstXI CCANNNNNNTGG 2 cut(s) 60, 2177
BstYI RGATCY 4 cut(s) 1148, 1919, 2446, 2599
BstZ17I GTATAC 1 cut(s) 716
BsuI GTATCC 3 cut(s) 276, 803, 1761
BsuRI GGCC 5 cut(s) 1203, 1352, 1959, 2373, 2473
BtgI CCRYGG 2 cut(s) 98, 1754
BtrI CACGTC 1 cut(s) 2057
BtsCI GGATG 8 cut(s) 258, 1039, 1173, 2028, 2114, 2421, 2737, 2860
BtsI GCAGTG 1 cut(s) 2866
BtsIMutI CAGTG 6 cut(s) 126, 461, 521, 843, 1184, 2866
BveI ACCTGC 2 cut(s) 28, 1785
Cac8I GCNNGC 1 cut(s) 2491
CaiI CAGNNNCTG 1 cut(s) 866
Cfr13I GGNCC 3 cut(s) 597, 791, 2471
CseI GACGC 3 cut(s) 36, 700, 1940
Csp6I GTAC 5 cut(s) 178, 645, 761, 1022, 1731
CviQI GTAC 5 cut(s) 178, 645, 761, 1022, 1731
DdeI CTNAG 6 cut(s) 338, 515, 539, 1497, 2094, 2636
DpnI GATC 8 cut(s) 321, 1150, 1921, 1984, 2225, 2448, 2601, 2652
DpnII GATC 8 cut(s) 319, 1148, 1919, 1982, 2223, 2446, 2599, 2650
DrdI GACNNNNNNGTC 1 cut(s) 897
DseDI GACNNNNNNGTC 1 cut(s) 897
EaeI YGGCCR 2 cut(s) 1201, 2371
Eam1104I CTCTTC 4 cut(s) 1374, 1518, 2511, 2815
EarI CTCTTC 4 cut(s) 1374, 1518, 2511, 2815
Eco130I CCWWGG 3 cut(s) 98, 593, 1754
Eco147I AGGCCT 1 cut(s) 1352
Eco24I GRGCYC 1 cut(s) 191
Eco31I GGTCTC 2 cut(s) 2325, 2896
Eco32I GATATC 1 cut(s) 1966
Eco47I GGWCC 2 cut(s) 597, 791
Eco57I CTGAAG 2 cut(s) 1398, 1707
EcoO109I RGGNCCY 1 cut(s) 597
EcoRI GAATTC 1 cut(s) 69
EcoRII CCWGG 6 cut(s) 1198, 1826, 1959, 2133, 2794, 2886
EcoRV GATATC 1 cut(s) 1966
EcoT14I CCWWGG 3 cut(s) 98, 593, 1754
EcoT22I ATGCAT 1 cut(s) 1778
EcoT38I GRGCYC 1 cut(s) 191
ErhI CCWWGG 3 cut(s) 98, 593, 1754
Esp3I CGTCTC 1 cut(s) 683
FalI AAGNNNNNCTT 6 cut(s) 321, 353, 1074, 1106, 1290, 1322
FaqI GGGAC 2 cut(s) 777, 1853
FauNDI CATATG 2 cut(s) 2156, 2239
FbaI TGATCA 2 cut(s) 319, 2650
FblI GTMKAC 3 cut(s) 5, 715, 1235
Fnu4HI GCNGC 8 cut(s) 137, 509, 998, 1059, 1431, 1434, 1851, 2073
FokI GGATG 8 cut(s) 265, 1026, 1180, 2015, 2121, 2428, 2744, 2867
FriOI GRGCYC 1 cut(s) 191
Fsp4HI GCNGC 8 cut(s) 137, 509, 998, 1059, 1431, 1434, 1851, 2073
FspBI CTAG 7 cut(s) 1239, 1517, 1620, 1656, 1734, 1854, 2261
GluI GCNGC 8 cut(s) 137, 509, 998, 1059, 1431, 1434, 1851, 2073
GsaI CCCAGC 1 cut(s) 2472
GsuI CTGGAG 2 cut(s) 843, 2505
HaeIII GGCC 5 cut(s) 1203, 1352, 1959, 2373, 2473
HapII CCGG 1 cut(s) 2700
HgaI GACGC 3 cut(s) 36, 700, 1940
Hin1I GRCGYC 1 cut(s) 28
HincII GTYRAC 5 cut(s) 6, 1174, 1252, 1912, 1930
HindII GTYRAC 5 cut(s) 6, 1174, 1252, 1912, 1930
HindIII AAGCTT 2 cut(s) 310, 1499
HpaII CCGG 1 cut(s) 2700
HphI GGTGA 9 cut(s) 9, 557, 650, 932, 959, 1843, 1929, 2096, 2192
Hpy188I TCNGA 7 cut(s) 820, 1148, 1390, 1561, 1567, 2650, 2715
Hpy99I CGWCG 1 cut(s) 30
HpyAV CCTTC 6 cut(s) 488, 764, 960, 1653, 2045, 2735
HpyCH4III ACNGT 5 cut(s) 121, 847, 1641, 2210, 2246
HpyCH4IV ACGT 2 cut(s) 2056, 2396
HpyF10VI GCNNNNNNNGC 4 cut(s) 994, 1265, 1442, 2703
HpyF3I CTNAG 6 cut(s) 338, 515, 539, 1497, 2094, 2636
HpySE526I ACGT 2 cut(s) 2056, 2396
Hsp92I GRCGYC 1 cut(s) 28
Kpn2I TCCGGA 1 cut(s) 2699
Ksp22I TGATCA 2 cut(s) 319, 2650
Kzo9I GATC 8 cut(s) 319, 1148, 1919, 1982, 2223, 2446, 2599, 2650
LguI GCTCTTC 2 cut(s) 1518, 2511
LmnI GCTCC 4 cut(s) 621, 838, 2486, 2702
Lsp1109I GCAGC 7 cut(s) 148, 520, 1009, 1045, 1417, 1420, 2084
LweI GCATC 5 cut(s) 243, 1045, 1943, 2099, 2722
MaeI CTAG 7 cut(s) 1239, 1517, 1620, 1656, 1734, 1854, 2261
MaeII ACGT 2 cut(s) 2056, 2396
MalI GATC 8 cut(s) 321, 1150, 1921, 1984, 2225, 2448, 2601, 2652
MboI GATC 8 cut(s) 319, 1148, 1919, 1982, 2223, 2446, 2599, 2650
MfeI CAATTG 3 cut(s) 54, 708, 2219
MflI RGATCY 4 cut(s) 1148, 1919, 2446, 2599
MhlI GDGCHC 4 cut(s) 191, 626, 2634, 2868
MlsI TGGCCA 2 cut(s) 1203, 2373
MluNI TGGCCA 2 cut(s) 1203, 2373
MlyI GAGTC 2 cut(s) 43, 119
MmeI TCCRAC 4 cut(s) 1145, 1511, 2086, 2766
Mox20I TGGCCA 2 cut(s) 1203, 2373
Mph1103I ATGCAT 1 cut(s) 1778
MroI TCCGGA 1 cut(s) 2699
MroXI GAANNNNTTC 3 cut(s) 401, 1224, 2023
MscI TGGCCA 2 cut(s) 1203, 2373
MslI CAYNNNNRTG 4 cut(s) 629, 945, 1044, 2280
Msp20I TGGCCA 2 cut(s) 1203, 2373
MspI CCGG 1 cut(s) 2700
MspR9I CCNGG 6 cut(s) 1200, 1828, 1961, 2135, 2796, 2888
MunI CAATTG 3 cut(s) 54, 708, 2219
MvaI CCWGG 6 cut(s) 1200, 1828, 1961, 2135, 2796, 2888
MwoI GCNNNNNNNGC 4 cut(s) 994, 1265, 1442, 2703
NcoI CCATGG 2 cut(s) 98, 1754
NdeI CATATG 2 cut(s) 2156, 2239
NdeII GATC 8 cut(s) 319, 1148, 1919, 1982, 2223, 2446, 2599, 2650
NlaIV GGNNCC 5 cut(s) 793, 1921, 2026, 2488, 2601
NmuCI GTSAC 4 cut(s) 115, 947, 2084, 2190
NsiI ATGCAT 1 cut(s) 1778
NspI RCATGY 3 cut(s) 1597, 1785, 2425
OliI CACNNNNGTG 1 cut(s) 629
PaqCI CACCTGC 2 cut(s) 28, 1785
PceI AGGCCT 1 cut(s) 1352
PciI ACATGT 2 cut(s) 1593, 1781
PciSI GCTCTTC 2 cut(s) 1518, 2511
PdmI GAANNNNTTC 3 cut(s) 401, 1224, 2023
PflMI CCANNNNNTGG 4 cut(s) 275, 1745, 2034, 2303
PfoI TCCNGGA 1 cut(s) 2133
PkrI GCNGC 8 cut(s) 138, 510, 999, 1060, 1432, 1435, 1852, 2074
PleI GAGTC 2 cut(s) 43, 118
PpsI GAGTC 2 cut(s) 43, 118
PpuMI RGGWCCY 1 cut(s) 597
PscI ACATGT 2 cut(s) 1593, 1781
PsiI TTATAA 3 cut(s) 1112, 1214, 2268
Psp1406I AACGTT 1 cut(s) 2396
Psp5II RGGWCCY 1 cut(s) 597
Psp6I CCWGG 6 cut(s) 1198, 1826, 1959, 2133, 2794, 2886
PspFI CCCAGC 1 cut(s) 2468
PspGI CCWGG 6 cut(s) 1198, 1826, 1959, 2133, 2794, 2886
PspN4I GGNNCC 5 cut(s) 793, 1921, 2026, 2488, 2601
PspPI GGNCC 3 cut(s) 597, 791, 2471
PspPPI RGGWCCY 1 cut(s) 597
PstNI CAGNNNCTG 1 cut(s) 866
PsuI RGATCY 4 cut(s) 1148, 1919, 2446, 2599
RsaI GTAC 5 cut(s) 179, 646, 762, 1023, 1732
RsaNI GTAC 5 cut(s) 178, 645, 761, 1022, 1731
RseI CAYNNNNRTG 4 cut(s) 629, 945, 1044, 2280
SalI GTCGAC 1 cut(s) 4
SapI GCTCTTC 2 cut(s) 1518, 2511
SatI GCNGC 8 cut(s) 137, 509, 998, 1059, 1431, 1434, 1851, 2073
Sau3AI GATC 8 cut(s) 319, 1148, 1919, 1982, 2223, 2446, 2599, 2650
Sau96I GGNCC 3 cut(s) 597, 791, 2471
ScaI AGTACT 1 cut(s) 1732
SchI GAGTC 2 cut(s) 43, 119
ScrFI CCNGG 6 cut(s) 1200, 1828, 1961, 2135, 2796, 2888
SduI GDGCHC 4 cut(s) 191, 626, 2634, 2868
SfaNI GCATC 5 cut(s) 243, 1045, 1943, 2099, 2722
SfcI CTRYAG 2 cut(s) 422, 720
SinI GGWCC 2 cut(s) 597, 791
SmiMI CAYNNNNRTG 4 cut(s) 629, 945, 1044, 2280
SpeI ACTAGT 2 cut(s) 1238, 1733
SseBI AGGCCT 1 cut(s) 1352
SsiI CCGC 5 cut(s) 543, 945, 976, 1850, 2671
SspI AATATT 1 cut(s) 1143
SspMI CTAG 7 cut(s) 1239, 1517, 1620, 1656, 1734, 1854, 2261
StuI AGGCCT 1 cut(s) 1352
StyD4I CCNGG 6 cut(s) 1198, 1826, 1959, 2133, 2794, 2886
StyI CCWWGG 3 cut(s) 98, 593, 1754
TaaI ACNGT 5 cut(s) 121, 847, 1641, 2210, 2246
TaiI ACGT 2 cut(s) 2059, 2399
TaqI TCGA 7 cut(s) 5, 191, 414, 496, 2556, 2562, 2625
TatI WGTACW 5 cut(s) 177, 644, 760, 1021, 1730
TauI GCSGC 1 cut(s) 1853
TscAI CASTG 6 cut(s) 126, 468, 528, 850, 1191, 2873
TseFI GTSAC 4 cut(s) 115, 947, 2084, 2190
TseI GCWGC 7 cut(s) 136, 508, 997, 1058, 1430, 1433, 2072
Tsp45I GTSAC 4 cut(s) 115, 947, 2084, 2190
TspGWI ACGGA 1 cut(s) 641
TspRI CASTG 6 cut(s) 126, 468, 528, 850, 1191, 2873
Van91I CCANNNNNTGG 4 cut(s) 275, 1745, 2034, 2303
VneI GTGCAC 1 cut(s) 2864
VpaK11BI GGWCC 2 cut(s) 597, 791
XapI RAATTY 7 cut(s) 69, 332, 526, 777, 2758, 2774, 2835
XbaI TCTAGA 1 cut(s) 1516
XceI RCATGY 3 cut(s) 1597, 1785, 2425
XcmI CCANNNNNNNNNTGG 1 cut(s) 1304
XmiI GTMKAC 3 cut(s) 5, 715, 1235
XmnI GAANNNNTTC 3 cut(s) 401, 1224, 2023
XspI CTAG 7 cut(s) 1239, 1517, 1620, 1656, 1734, 1854, 2261
ZrmI AGTACT 1 cut(s) 1732
Zsp2I ATGCAT 1 cut(s) 1778
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.