RchiOBHm_Chr3g0495421

protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0007548
Physical Location & Seq
Forward (+)
44937638 .. 44938786
1149 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ45784

Sequence Viewer

Length: 600 bp
ATGAGATTTTTTTTTCCAGGTGTTCTTGAGAAAACTGGAGTTGAGCCACAAGTGAAAAGAATTGGAAAATATGAAAGTTTTGGTGAGCAACTTGCGCATATGACCATGTCCGAAGAAAATTGTGAGATGTTGACAGCATTGCTCGATAACATATATGGGAACTGGCTGGACATAATTTCTTCTACAAGAGTTGATGTGAAAGTCATCAGGGGAGTGGTTGAAGCATTTAACCTGGGAAAACTATATGAGAAGATTGGCTTCAACAAGGAAATCATATCAAGGGGGAATTTGCAGAGGTTCTTGACAAGGCAGCTTTTTCCGGCCAGATCAATGACTGTAGATAAAATGGAGGAAGTTGCACAAGGGAGAGTTTGGGCAGGTAAATATACAACTTCAAGGGGTTTAGTCGATGCTATTGGTGGACTTTCTCGAGCTGTTGCGATAGCAAAGCTCAAGGCAAATATACCACAAGACAGAGAGGTTGGTTACACTAGTGGAACTTGCAAGACCATCCCCTTCACTGCCAGAACTTTTATGTGGCGTGGGGAGTACTCTGGTGGGAGTTGCTTTCCAAGAATGGCCAAGAAATTCAATGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S49

Protein Analysis

199

Amino Acids

22.38

Weight (kDa)

9.59

Isoelectric Point (pI)

32.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S49 PF01343 108 - 154 2.1e-06 Peptidase family S49
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 96
Acc36I ACCTGC 1 cut(s) 368
AcoI YGGCCR 2 cut(s) 321, 579
AcsI RAATTY 2 cut(s) 286, 587
AfaI GTAC 1 cut(s) 551
AgsI TTSAA 4 cut(s) 221, 262, 396, 592
AhlI ACTAGT 1 cut(s) 491
AjnI CCWGG 2 cut(s) 16, 231
AluBI AGCT 3 cut(s) 313, 434, 451
AluI AGCT 3 cut(s) 313, 434, 451
Ama87I CYCGRG 1 cut(s) 429
AoxI GGCC 2 cut(s) 321, 579
ApeKI GCWGC 1 cut(s) 310
ApoI RAATTY 2 cut(s) 286, 587
AspLEI GCGC 1 cut(s) 97
AsuHPI GGTGA 1 cut(s) 95
AvaI CYCGRG 1 cut(s) 429
BalI TGGCCA 1 cut(s) 581
BbvI GCAGC 1 cut(s) 322
BccI CCATC 1 cut(s) 518
BciT130I CCWGG 2 cut(s) 18, 233
BcuI ACTAGT 1 cut(s) 491
BfaI CTAG 1 cut(s) 492
BfmI CTRYAG 1 cut(s) 336
BfuAI ACCTGC 1 cut(s) 368
BisI GCNGC 1 cut(s) 311
BlsI GCNGC 1 cut(s) 312
BmcAI AGTACT 1 cut(s) 551
Bme1390I CCNGG 2 cut(s) 18, 233
BmeT110I CYCGRG 1 cut(s) 429
BmrFI CCNGG 2 cut(s) 18, 233
BmsI GCATC 1 cut(s) 400
BpmI CTGGAG 1 cut(s) 57
BpuEI CTTGAG 2 cut(s) 47, 437
BsaJI CCNNGG 1 cut(s) 232
Bse1I ACTGG 2 cut(s) 40, 167
Bse3DI GCAATG 1 cut(s) 137
BseBI CCWGG 2 cut(s) 18, 233
BseDI CCNNGG 1 cut(s) 232
BseGI GGATG 1 cut(s) 510
BseMI GCAATG 1 cut(s) 137
BseNI ACTGG 2 cut(s) 40, 167
BseXI GCAGC 1 cut(s) 322
BshFI GGCC 2 cut(s) 323, 581
BsiHKCI CYCGRG 1 cut(s) 429
BsiSI CCGG 1 cut(s) 320
BsnI GGCC 2 cut(s) 323, 581
BsoBI CYCGRG 1 cut(s) 429
Bsp143I GATC 1 cut(s) 326
BspANI GGCC 2 cut(s) 323, 581
BspMI ACCTGC 1 cut(s) 368
BsrDI GCAATG 1 cut(s) 137
BsrI ACTGG 2 cut(s) 40, 167
BssECI CCNNGG 1 cut(s) 232
BssMI GATC 1 cut(s) 326
Bst2UI CCWGG 2 cut(s) 18, 233
Bst4CI ACNGT 1 cut(s) 337
BstF5I GGATG 1 cut(s) 510
BstHHI GCGC 1 cut(s) 97
BstKTI GATC 1 cut(s) 329
BstMBI GATC 1 cut(s) 326
BstMWI GCNNNNNNNGC 1 cut(s) 94
BstNI CCWGG 2 cut(s) 18, 233
BstSCI CCNGG 2 cut(s) 16, 231
BstSFI CTRYAG 1 cut(s) 336
BstV1I GCAGC 1 cut(s) 322
BsuRI GGCC 2 cut(s) 323, 581
BtsCI GGATG 1 cut(s) 510
BtsI GCAGTG 1 cut(s) 519
BtsIMutI CAGTG 1 cut(s) 519
BveI ACCTGC 1 cut(s) 368
CfoI GCGC 1 cut(s) 97
Csp6I GTAC 1 cut(s) 550
CviAII CATG 1 cut(s) 106
CviJI RGCY 8 cut(s) 46, 166, 258, 313, 323, 434, 451, 581
CviKI_1 RGCY 8 cut(s) 46, 166, 258, 313, 323, 434, 451, 581
CviQI GTAC 1 cut(s) 550
DpnI GATC 1 cut(s) 328
DpnII GATC 1 cut(s) 326
EaeI YGGCCR 2 cut(s) 321, 579
Eco88I CYCGRG 1 cut(s) 429
EcoRII CCWGG 2 cut(s) 16, 231
FaeI CATG 1 cut(s) 109
FalI AAGNNNNNCTT 2 cut(s) 242, 274
FatI CATG 1 cut(s) 105
FauNDI CATATG 1 cut(s) 99
Fnu4HI GCNGC 1 cut(s) 311
FokI GGATG 1 cut(s) 497
Fsp4HI GCNGC 1 cut(s) 311
FspBI CTAG 1 cut(s) 492
FspI TGCGCA 1 cut(s) 96
GlaI GCGC 1 cut(s) 96
GluI GCNGC 1 cut(s) 311
GsuI CTGGAG 1 cut(s) 57
HaeIII GGCC 2 cut(s) 323, 581
HapII CCGG 1 cut(s) 320
HhaI GCGC 1 cut(s) 97
Hin1II CATG 1 cut(s) 109
Hin6I GCGC 1 cut(s) 95
HinP1I GCGC 1 cut(s) 95
HincII GTYRAC 1 cut(s) 132
HindII GTYRAC 1 cut(s) 132
HpaII CCGG 1 cut(s) 320
HphI GGTGA 1 cut(s) 95
Hpy166II GTNNAC 2 cut(s) 132, 422
Hpy188I TCNGA 1 cut(s) 112
Hpy188III TCNNGA 3 cut(s) 26, 301, 429
Hpy8I GTNNAC 2 cut(s) 132, 422
HpyAV CCTTC 1 cut(s) 526
HpyCH4III ACNGT 1 cut(s) 337
HpyCH4V TGCA 3 cut(s) 292, 359, 504
HpyF10VI GCNNNNNNNGC 1 cut(s) 94
Hsp92II CATG 1 cut(s) 109
HspAI GCGC 1 cut(s) 95
Kzo9I GATC 1 cut(s) 326
Lsp1109I GCAGC 1 cut(s) 322
LweI GCATC 1 cut(s) 400
MaeI CTAG 1 cut(s) 492
MaeIII GTNAC 1 cut(s) 485
MalI GATC 1 cut(s) 328
MboI GATC 1 cut(s) 326
MboII GAAGA 3 cut(s) 125, 171, 262
MlsI TGGCCA 1 cut(s) 581
MluCI AATT 5 cut(s) 60, 118, 174, 286, 587
MluNI TGGCCA 1 cut(s) 581
MnlI CCTC 3 cut(s) 288, 343, 472
Mox20I TGGCCA 1 cut(s) 581
MscI TGGCCA 1 cut(s) 581
MseI TTAA 1 cut(s) 228
Msp20I TGGCCA 1 cut(s) 581
MspI CCGG 1 cut(s) 320
MspR9I CCNGG 2 cut(s) 18, 233
MvaI CCWGG 2 cut(s) 18, 233
MwoI GCNNNNNNNGC 1 cut(s) 94
NdeI CATATG 1 cut(s) 99
NdeII GATC 1 cut(s) 326
NlaIII CATG 1 cut(s) 109
NsbI TGCGCA 1 cut(s) 96
PaeR7I CTCGAG 1 cut(s) 429
PflFI GACNNNGTC 1 cut(s) 106
PkrI GCNGC 1 cut(s) 312
Psp6I CCWGG 2 cut(s) 16, 231
PspGI CCWGG 2 cut(s) 16, 231
PsyI GACNNNGTC 1 cut(s) 106
RsaI GTAC 1 cut(s) 551
RsaNI GTAC 1 cut(s) 550
SaqAI TTAA 1 cut(s) 228
SatI GCNGC 1 cut(s) 311
Sau3AI GATC 1 cut(s) 326
ScaI AGTACT 1 cut(s) 551
ScrFI CCNGG 2 cut(s) 18, 233
SetI ASST 8 cut(s) 22, 234, 299, 315, 382, 436, 453, 483
SfaNI GCATC 1 cut(s) 400
SfcI CTRYAG 1 cut(s) 336
Sfr274I CTCGAG 1 cut(s) 429
SlaI CTCGAG 1 cut(s) 429
SmlI CTYRAG 3 cut(s) 26, 429, 452
SmoI CTYRAG 3 cut(s) 26, 429, 452
SpeI ACTAGT 1 cut(s) 491
Sse9I AATT 5 cut(s) 60, 118, 174, 286, 587
SspMI CTAG 1 cut(s) 492
StyD4I CCNGG 2 cut(s) 16, 231
TaaI ACNGT 1 cut(s) 337
TaqI TCGA 3 cut(s) 144, 408, 430
TasI AATT 5 cut(s) 60, 118, 174, 286, 587
TatI WGTACW 1 cut(s) 549
Tru1I TTAA 1 cut(s) 228
Tru9I TTAA 1 cut(s) 228
TscAI CASTG 1 cut(s) 526
TseI GCWGC 1 cut(s) 310
TspDTI ATGAA 1 cut(s) 87
TspRI CASTG 1 cut(s) 526
Tth111I GACNNNGTC 1 cut(s) 106
XapI RAATTY 2 cut(s) 286, 587
XhoI CTCGAG 1 cut(s) 429
XspI CTAG 1 cut(s) 492
ZrmI AGTACT 1 cut(s) 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.