RLG00000035479

protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0007548
Physical Location & Seq
Forward (+)
67360538 .. 67362485
1948 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035479

Sequence Viewer

Length: 513 bp
ATGGGAATTAGGCCCAAGAGAATGGTGGTCTCCGATGAGAGCCTTAAGTTGCTTTCCAGGTATGGCCAAGAAATTATGGCAGTGAAGGGGAGGGTCTTGCAAGTTCCACCAATGTTCAGGCATCGTGTTCTTGAGAAAACTGGAGTTGAGCCACAAGTGGAGAGGATTAGTAAATACGAAAGTGCTGAAAATTGTGTGATGCTGACAGCATTGCTCGATAACATATATGGGAACTGGCTGGACATAATTTCTTCTACAAGAGAGGAAGCTAAACTCTTTGCTAAGTCTACACAGAATTCGTATAAGCAATTTCAGGACAAGGCAGCTTCTTCCAGATCAATGACTGTATATAAAATGGAGGAAGTTGCACAAGGCAAATATACCACAAGACACAGAGGTTACACTAGTGGAACTTGCAAGACCATCCCCTTCACTGCCAAAACTTTTAAGTGGTGTAGGGAGTACTCTGGTGGGAGTTGCTTTCTAGGTATGGCGAAGAAATTCAATGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S49

Protein Analysis

171

Amino Acids

19.39

Weight (kDa)

9.67

Isoelectric Point (pI)

35.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 287
AcoI YGGCCR 1 cut(s) 64
AcsI RAATTY 2 cut(s) 295, 500
AfaI GTAC 1 cut(s) 464
AflII CTTAAG 1 cut(s) 44
AgsI TTSAA 1 cut(s) 505
AhlI ACTAGT 1 cut(s) 404
AjnI CCWGG 1 cut(s) 56
AluBI AGCT 2 cut(s) 269, 326
AluI AGCT 2 cut(s) 269, 326
Alw26I GTCTC 1 cut(s) 34
AoxI GGCC 2 cut(s) 11, 64
ApeKI GCWGC 1 cut(s) 323
ApoI RAATTY 2 cut(s) 295, 500
Asp700I GAANNNNTTC 1 cut(s) 500
AspS9I GGNCC 1 cut(s) 12
BalI TGGCCA 1 cut(s) 66
BbvI GCAGC 1 cut(s) 335
BccI CCATC 1 cut(s) 431
BciT130I CCWGG 1 cut(s) 58
BcoDI GTCTC 1 cut(s) 34
BcuI ACTAGT 1 cut(s) 404
BfaI CTAG 2 cut(s) 405, 485
BfrI CTTAAG 1 cut(s) 44
BisI GCNGC 1 cut(s) 324
BlsI GCNGC 1 cut(s) 325
BmcAI AGTACT 1 cut(s) 464
Bme1390I CCNGG 1 cut(s) 58
BmgT120I GGNCC 1 cut(s) 12
BmrFI CCNGG 1 cut(s) 58
BmsI GCATC 2 cut(s) 130, 189
BpmI CTGGAG 1 cut(s) 162
BpuEI CTTGAG 1 cut(s) 152
BsaI GGTCTC 1 cut(s) 34
Bse1I ACTGG 2 cut(s) 145, 239
Bse3DI GCAATG 1 cut(s) 209
BseBI CCWGG 1 cut(s) 58
BseGI GGATG 1 cut(s) 423
BseMI GCAATG 1 cut(s) 209
BseNI ACTGG 2 cut(s) 145, 239
BseXI GCAGC 1 cut(s) 335
BshFI GGCC 2 cut(s) 13, 66
BsmAI GTCTC 1 cut(s) 34
BsnI GGCC 2 cut(s) 13, 66
Bso31I GGTCTC 1 cut(s) 34
Bsp143I GATC 1 cut(s) 335
BspANI GGCC 2 cut(s) 13, 66
BspTI CTTAAG 1 cut(s) 44
BspTNI GGTCTC 1 cut(s) 34
BsrDI GCAATG 1 cut(s) 209
BsrI ACTGG 2 cut(s) 145, 239
BssMI GATC 1 cut(s) 335
Bst2UI CCWGG 1 cut(s) 58
Bst4CI ACNGT 1 cut(s) 346
BstAFI CTTAAG 1 cut(s) 44
BstDEI CTNAG 1 cut(s) 282
BstF5I GGATG 1 cut(s) 423
BstKTI GATC 1 cut(s) 338
BstMAI GTCTC 1 cut(s) 34
BstMBI GATC 1 cut(s) 335
BstNI CCWGG 1 cut(s) 58
BstSCI CCNGG 1 cut(s) 56
BstV1I GCAGC 1 cut(s) 335
BstXI CCANNNNNNTGG 1 cut(s) 22
BsuRI GGCC 2 cut(s) 13, 66
BtsCI GGATG 1 cut(s) 423
BtsI GCAGTG 2 cut(s) 87, 432
BtsIMutI CAGTG 2 cut(s) 87, 432
Cfr13I GGNCC 1 cut(s) 12
Csp6I GTAC 1 cut(s) 463
CviJI RGCY 7 cut(s) 13, 42, 66, 151, 238, 269, 326
CviKI_1 RGCY 7 cut(s) 13, 42, 66, 151, 238, 269, 326
CviQI GTAC 1 cut(s) 463
DdeI CTNAG 1 cut(s) 282
DpnI GATC 1 cut(s) 337
DpnII GATC 1 cut(s) 335
EaeI YGGCCR 1 cut(s) 64
Eco31I GGTCTC 1 cut(s) 34
EcoRI GAATTC 1 cut(s) 295
EcoRII CCWGG 1 cut(s) 56
FblI GTMKAC 1 cut(s) 287
Fnu4HI GCNGC 1 cut(s) 324
FokI GGATG 1 cut(s) 410
Fsp4HI GCNGC 1 cut(s) 324
FspBI CTAG 2 cut(s) 405, 485
GluI GCNGC 1 cut(s) 324
GsuI CTGGAG 1 cut(s) 162
HaeIII GGCC 2 cut(s) 13, 66
Hpy166II GTNNAC 1 cut(s) 288
Hpy188I TCNGA 1 cut(s) 34
Hpy188III TCNNGA 3 cut(s) 131, 314, 333
Hpy8I GTNNAC 1 cut(s) 288
HpyAV CCTTC 2 cut(s) 79, 439
HpyCH4III ACNGT 1 cut(s) 346
HpyCH4V TGCA 3 cut(s) 100, 368, 417
HpyF3I CTNAG 1 cut(s) 282
Kzo9I GATC 1 cut(s) 335
LpnPI CCDG 9 cut(s) 43, 70, 103, 126, 220, 224, 299, 346, 453
Lsp1109I GCAGC 1 cut(s) 335
LweI GCATC 2 cut(s) 130, 189
MaeI CTAG 2 cut(s) 405, 485
MaeIII GTNAC 1 cut(s) 398
MalI GATC 1 cut(s) 337
MboI GATC 1 cut(s) 335
MboII GAAGA 3 cut(s) 243, 321, 508
MlsI TGGCCA 1 cut(s) 66
MluCI AATT 7 cut(s) 6, 72, 190, 246, 295, 308, 500
MluNI TGGCCA 1 cut(s) 66
MnlI CCTC 5 cut(s) 84, 156, 256, 352, 389
Mox20I TGGCCA 1 cut(s) 66
MroXI GAANNNNTTC 1 cut(s) 500
MscI TGGCCA 1 cut(s) 66
MseI TTAA 2 cut(s) 45, 447
Msp20I TGGCCA 1 cut(s) 66
MspCI CTTAAG 1 cut(s) 44
MspR9I CCNGG 1 cut(s) 58
MvaI CCWGG 1 cut(s) 58
NdeII GATC 1 cut(s) 335
PdmI GAANNNNTTC 1 cut(s) 500
PkrI GCNGC 1 cut(s) 325
Psp6I CCWGG 1 cut(s) 56
PspGI CCWGG 1 cut(s) 56
PspPI GGNCC 1 cut(s) 12
RsaI GTAC 1 cut(s) 464
RsaNI GTAC 1 cut(s) 463
SaqAI TTAA 2 cut(s) 45, 447
SatI GCNGC 1 cut(s) 324
Sau3AI GATC 1 cut(s) 335
Sau96I GGNCC 1 cut(s) 12
ScaI AGTACT 1 cut(s) 464
ScrFI CCNGG 1 cut(s) 58
SetI ASST 5 cut(s) 62, 271, 328, 400, 490
SfaNI GCATC 2 cut(s) 130, 189
SmlI CTYRAG 2 cut(s) 44, 131
SmoI CTYRAG 2 cut(s) 44, 131
SpeI ACTAGT 1 cut(s) 404
Sse9I AATT 7 cut(s) 6, 72, 190, 246, 295, 308, 500
SspMI CTAG 2 cut(s) 405, 485
StyD4I CCNGG 1 cut(s) 56
TaaI ACNGT 1 cut(s) 346
TaqI TCGA 1 cut(s) 216
TasI AATT 7 cut(s) 6, 72, 190, 246, 295, 308, 500
TatI WGTACW 1 cut(s) 462
Tru1I TTAA 2 cut(s) 45, 447
Tru9I TTAA 2 cut(s) 45, 447
TscAI CASTG 2 cut(s) 87, 439
TseI GCWGC 1 cut(s) 323
TspRI CASTG 2 cut(s) 87, 439
Vha464I CTTAAG 1 cut(s) 44
XapI RAATTY 2 cut(s) 295, 500
XcmI CCANNNNNNNNNTGG 1 cut(s) 22
XmiI GTMKAC 1 cut(s) 287
XmnI GAANNNNTTC 1 cut(s) 500
XspI CTAG 2 cut(s) 405, 485
ZrmI AGTACT 1 cut(s) 464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.