riboflavin kinase
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 3 cut(s) | 847, 860, 1129 |
| AcsI | RAATTY | 5 cut(s) | 129, 460, 1190, 1239, 1248 |
| AcuI | CTGAAG | 1 cut(s) | 678 |
| AdeI | CACNNNGTG | 1 cut(s) | 410 |
| AfaI | GTAC | 4 cut(s) | 73, 272, 351, 660 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 64, 1115, 1225 |
| AflIII | ACRYGT | 1 cut(s) | 944 |
| AjnI | CCWGG | 3 cut(s) | 368, 613, 793 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 225, 257 |
| AluBI | AGCT | 9 cut(s) | 164, 230, 383, 557, 588, 863, 1112, 1122, 1166 |
| AluI | AGCT | 9 cut(s) | 164, 230, 383, 557, 588, 863, 1112, 1122, 1166 |
| AlwI | GGATC | 3 cut(s) | 847, 860, 1129 |
| Ama87I | CYCGRG | 2 cut(s) | 910, 1115 |
| AoxI | GGCC | 3 cut(s) | 745, 1091, 1098 |
| ApeKI | GCWGC | 4 cut(s) | 164, 557, 635, 1024 |
| ApoI | RAATTY | 5 cut(s) | 129, 460, 1190, 1239, 1248 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 793, 825 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 14, 1065 |
| AspS9I | GGNCC | 3 cut(s) | 233, 745, 809 |
| AsuHPI | GGTGA | 3 cut(s) | 427, 521, 718 |
| AsuII | TTCGAA | 1 cut(s) | 900 |
| AvaI | CYCGRG | 2 cut(s) | 910, 1115 |
| AvaII | GGWCC | 2 cut(s) | 233, 809 |
| AxyI | CCTNAGG | 1 cut(s) | 1095 |
| BamHI | GGATCC | 1 cut(s) | 852 |
| BanII | GRGCYC | 1 cut(s) | 839 |
| BbvI | GCAGC | 4 cut(s) | 151, 544, 622, 1011 |
| BccI | CCATC | 5 cut(s) | 62, 113, 313, 541, 690 |
| BciT130I | CCWGG | 3 cut(s) | 370, 615, 795 |
| BclI | TGATCA | 1 cut(s) | 708 |
| BfmI | CTRYAG | 2 cut(s) | 858, 1011 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 632 |
| BisI | GCNGC | 4 cut(s) | 165, 558, 636, 1025 |
| BlsI | GCNGC | 4 cut(s) | 166, 559, 637, 1026 |
| Bme1390I | CCNGG | 3 cut(s) | 370, 615, 795 |
| Bme18I | GGWCC | 2 cut(s) | 233, 809 |
| BmeT110I | CYCGRG | 2 cut(s) | 910, 1115 |
| BmgT120I | GGNCC | 3 cut(s) | 233, 745, 809 |
| BmiI | GGNNCC | 4 cut(s) | 235, 374, 746, 854 |
| BmrFI | CCNGG | 3 cut(s) | 370, 615, 795 |
| BmsI | GCATC | 1 cut(s) | 1219 |
| Bpu14I | TTCGAA | 1 cut(s) | 900 |
| BsaAI | YACGTR | 2 cut(s) | 270, 346 |
| BsaBI | GATNNNNATC | 2 cut(s) | 17, 729 |
| BsaJI | CCNNGG | 4 cut(s) | 438, 794, 909, 1019 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 64, 1115, 1225 |
| Bse1I | ACTGG | 1 cut(s) | 627 |
| Bse21I | CCTNAGG | 1 cut(s) | 1095 |
| Bse3DI | GCAATG | 1 cut(s) | 304 |
| Bse8I | GATNNNNATC | 2 cut(s) | 17, 729 |
| BseBI | CCWGG | 3 cut(s) | 370, 615, 795 |
| BseDI | CCNNGG | 4 cut(s) | 438, 794, 909, 1019 |
| BseGI | GGATG | 6 cut(s) | 73, 124, 730, 904, 1204, 1234 |
| BseJI | GATNNNNATC | 2 cut(s) | 17, 729 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 64, 1115, 1225 |
| BseMI | GCAATG | 1 cut(s) | 304 |
| BseMII | CTCAG | 2 cut(s) | 238, 1086 |
| BseNI | ACTGG | 1 cut(s) | 627 |
| BseXI | GCAGC | 4 cut(s) | 151, 544, 622, 1011 |
| BseYI | CCCAGC | 1 cut(s) | 1108 |
| BsgI | GTGCAG | 1 cut(s) | 250 |
| BshFI | GGCC | 3 cut(s) | 747, 1093, 1100 |
| BsiHKCI | CYCGRG | 2 cut(s) | 910, 1115 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 64, 1115, 1225 |
| BsnI | GGCC | 3 cut(s) | 747, 1093, 1100 |
| BsoBI | CYCGRG | 2 cut(s) | 910, 1115 |
| Bsp119I | TTCGAA | 1 cut(s) | 900 |
| Bsp1286I | GDGCHC | 1 cut(s) | 839 |
| Bsp143I | GATC | 5 cut(s) | 521, 708, 852, 1134, 1171 |
| Bsp19I | CCATGG | 1 cut(s) | 1019 |
| BspANI | GGCC | 3 cut(s) | 747, 1093, 1100 |
| BspCNI | CTCAG | 2 cut(s) | 237, 1087 |
| BspHI | TCATGA | 2 cut(s) | 966, 1030 |
| BspLI | GGNNCC | 4 cut(s) | 235, 374, 746, 854 |
| BspPI | GGATC | 3 cut(s) | 847, 860, 1129 |
| BspT104I | TTCGAA | 1 cut(s) | 900 |
| BsrDI | GCAATG | 1 cut(s) | 304 |
| BsrI | ACTGG | 1 cut(s) | 627 |
| BssECI | CCNNGG | 4 cut(s) | 438, 794, 909, 1019 |
| BssMI | GATC | 5 cut(s) | 521, 708, 852, 1134, 1171 |
| BssT1I | CCWWGG | 2 cut(s) | 438, 1019 |
| Bst2UI | CCWGG | 3 cut(s) | 370, 615, 795 |
| Bst4CI | ACNGT | 2 cut(s) | 88, 582 |
| BstBAI | YACGTR | 2 cut(s) | 270, 346 |
| BstBI | TTCGAA | 1 cut(s) | 900 |
| BstDEI | CTNAG | 3 cut(s) | 224, 493, 1095 |
| BstDSI | CCRYGG | 1 cut(s) | 1019 |
| BstF5I | GGATG | 6 cut(s) | 73, 124, 730, 904, 1204, 1234 |
| BstKTI | GATC | 5 cut(s) | 524, 711, 855, 1137, 1174 |
| BstMBI | GATC | 5 cut(s) | 521, 708, 852, 1134, 1171 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 170, 632, 1024, 1163 |
| BstNI | CCWGG | 3 cut(s) | 370, 615, 795 |
| BstSCI | CCNGG | 3 cut(s) | 368, 613, 793 |
| BstSFI | CTRYAG | 2 cut(s) | 858, 1011 |
| BstV1I | GCAGC | 4 cut(s) | 151, 544, 622, 1011 |
| BstX2I | RGATCY | 1 cut(s) | 852 |
| BstYI | RGATCY | 1 cut(s) | 852 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1095 |
| BsuRI | GGCC | 3 cut(s) | 747, 1093, 1100 |
| BtgI | CCRYGG | 1 cut(s) | 1019 |
| BtgZI | GCGATG | 1 cut(s) | 4 |
| BtsCI | GGATG | 6 cut(s) | 73, 124, 730, 904, 1204, 1234 |
| BtsIMutI | CAGTG | 1 cut(s) | 999 |
| CciI | TCATGA | 2 cut(s) | 966, 1030 |
| Cfr13I | GGNCC | 3 cut(s) | 233, 745, 809 |
| Csp6I | GTAC | 4 cut(s) | 72, 271, 350, 659 |
| CviAII | CATG | 4 cut(s) | 967, 1020, 1031, 1139 |
| CviQI | GTAC | 4 cut(s) | 72, 271, 350, 659 |
| DdeI | CTNAG | 3 cut(s) | 224, 493, 1095 |
| DpnI | GATC | 5 cut(s) | 523, 710, 854, 1136, 1173 |
| DpnII | GATC | 5 cut(s) | 521, 708, 852, 1134, 1171 |
| DraI | TTTAAA | 1 cut(s) | 958 |
| DraIII | CACNNNGTG | 1 cut(s) | 410 |
| Eco130I | CCWWGG | 2 cut(s) | 438, 1019 |
| Eco24I | GRGCYC | 1 cut(s) | 839 |
| Eco47I | GGWCC | 2 cut(s) | 233, 809 |
| Eco57I | CTGAAG | 1 cut(s) | 678 |
| Eco81I | CCTNAGG | 1 cut(s) | 1095 |
| Eco88I | CYCGRG | 2 cut(s) | 910, 1115 |
| EcoO109I | RGGNCCY | 1 cut(s) | 745 |
| EcoRII | CCWGG | 3 cut(s) | 368, 613, 793 |
| EcoT14I | CCWWGG | 2 cut(s) | 438, 1019 |
| EcoT38I | GRGCYC | 1 cut(s) | 839 |
| ErhI | CCWWGG | 2 cut(s) | 438, 1019 |
| FaeI | CATG | 4 cut(s) | 970, 1023, 1034, 1142 |
| FatI | CATG | 4 cut(s) | 966, 1019, 1030, 1138 |
| FauNDI | CATATG | 1 cut(s) | 1088 |
| FbaI | TGATCA | 1 cut(s) | 708 |
| Fnu4HI | GCNGC | 4 cut(s) | 165, 558, 636, 1025 |
| FokI | GGATG | 6 cut(s) | 80, 131, 717, 891, 1211, 1241 |
| FriOI | GRGCYC | 1 cut(s) | 839 |
| Fsp4HI | GCNGC | 4 cut(s) | 165, 558, 636, 1025 |
| GluI | GCNGC | 4 cut(s) | 165, 558, 636, 1025 |
| GsaI | CCCAGC | 1 cut(s) | 1112 |
| HaeIII | GGCC | 3 cut(s) | 747, 1093, 1100 |
| Hin1II | CATG | 4 cut(s) | 970, 1023, 1034, 1142 |
| HinfI | GANTC | 3 cut(s) | 485, 605, 982 |
| HphI | GGTGA | 3 cut(s) | 427, 521, 718 |
| Hpy166II | GTNNAC | 2 cut(s) | 74, 212 |
| Hpy188I | TCNGA | 1 cut(s) | 227 |
| Hpy8I | GTNNAC | 2 cut(s) | 74, 212 |
| HpyAV | CCTTC | 2 cut(s) | 118, 499 |
| HpyCH4III | ACNGT | 2 cut(s) | 88, 582 |
| HpyCH4IV | ACGT | 3 cut(s) | 269, 345, 944 |
| HpyCH4V | TGCA | 6 cut(s) | 167, 201, 267, 309, 1157, 1183 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 170, 632, 1024, 1163 |
| HpyF3I | CTNAG | 3 cut(s) | 224, 493, 1095 |
| HpySE526I | ACGT | 3 cut(s) | 269, 345, 944 |
| Hsp92II | CATG | 4 cut(s) | 970, 1023, 1034, 1142 |
| Ksp22I | TGATCA | 1 cut(s) | 708 |
| Kzo9I | GATC | 5 cut(s) | 521, 708, 852, 1134, 1171 |
| LmnI | GCTCC | 1 cut(s) | 1119 |
| Lsp1109I | GCAGC | 4 cut(s) | 151, 544, 622, 1011 |
| LweI | GCATC | 1 cut(s) | 1219 |
| MaeII | ACGT | 3 cut(s) | 269, 345, 944 |
| MaeIII | GTNAC | 3 cut(s) | 402, 509, 619 |
| MalI | GATC | 5 cut(s) | 523, 710, 854, 1136, 1173 |
| MboI | GATC | 5 cut(s) | 521, 708, 852, 1134, 1171 |
| MboII | GAAGA | 3 cut(s) | 22, 614, 1166 |
| MfeI | CAATTG | 1 cut(s) | 332 |
| MflI | RGATCY | 1 cut(s) | 852 |
| MhlI | GDGCHC | 1 cut(s) | 839 |
| MmeI | TCCRAC | 3 cut(s) | 124, 458, 959 |
| MroXI | GAANNNNTTC | 2 cut(s) | 14, 1065 |
| MseI | TTAA | 6 cut(s) | 32, 569, 819, 957, 993, 1212 |
| MslI | CAYNNNNRTG | 3 cut(s) | 360, 397, 1227 |
| MspA1I | CMGCKG | 1 cut(s) | 588 |
| MspR9I | CCNGG | 3 cut(s) | 370, 615, 795 |
| MunI | CAATTG | 1 cut(s) | 332 |
| MvaI | CCWGG | 3 cut(s) | 370, 615, 795 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 170, 632, 1024, 1163 |
| NcoI | CCATGG | 1 cut(s) | 1019 |
| NdeI | CATATG | 1 cut(s) | 1088 |
| NdeII | GATC | 5 cut(s) | 521, 708, 852, 1134, 1171 |
| NlaIII | CATG | 4 cut(s) | 970, 1023, 1034, 1142 |
| NlaIV | GGNNCC | 4 cut(s) | 235, 374, 746, 854 |
| NmuCI | GTSAC | 3 cut(s) | 402, 509, 619 |
| NspV | TTCGAA | 1 cut(s) | 900 |
| PagI | TCATGA | 2 cut(s) | 966, 1030 |
| PdmI | GAANNNNTTC | 2 cut(s) | 14, 1065 |
| PfeI | GAWTC | 3 cut(s) | 485, 605, 982 |
| PkrI | GCNGC | 4 cut(s) | 166, 559, 637, 1026 |
| Ppu21I | YACGTR | 2 cut(s) | 270, 346 |
| Psp6I | CCWGG | 3 cut(s) | 368, 613, 793 |
| PspFI | CCCAGC | 1 cut(s) | 1108 |
| PspGI | CCWGG | 3 cut(s) | 368, 613, 793 |
| PspN4I | GGNNCC | 4 cut(s) | 235, 374, 746, 854 |
| PspPI | GGNCC | 3 cut(s) | 233, 745, 809 |
| PsuI | RGATCY | 1 cut(s) | 852 |
| PvuII | CAGCTG | 1 cut(s) | 588 |
| RsaI | GTAC | 4 cut(s) | 73, 272, 351, 660 |
| RsaNI | GTAC | 4 cut(s) | 72, 271, 350, 659 |
| RseI | CAYNNNNRTG | 3 cut(s) | 360, 397, 1227 |
| SaqAI | TTAA | 6 cut(s) | 32, 569, 819, 957, 993, 1212 |
| SatI | GCNGC | 4 cut(s) | 165, 558, 636, 1025 |
| Sau3AI | GATC | 5 cut(s) | 521, 708, 852, 1134, 1171 |
| Sau96I | GGNCC | 3 cut(s) | 233, 745, 809 |
| ScrFI | CCNGG | 3 cut(s) | 370, 615, 795 |
| SduI | GDGCHC | 1 cut(s) | 839 |
| SfaNI | GCATC | 1 cut(s) | 1219 |
| SfcI | CTRYAG | 2 cut(s) | 858, 1011 |
| SfuI | TTCGAA | 1 cut(s) | 900 |
| SinI | GGWCC | 2 cut(s) | 233, 809 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 360, 397, 1227 |
| SspI | AATATT | 2 cut(s) | 574, 884 |
| StyD4I | CCNGG | 3 cut(s) | 368, 613, 793 |
| StyI | CCWWGG | 2 cut(s) | 438, 1019 |
| TaaI | ACNGT | 2 cut(s) | 88, 582 |
| TaiI | ACGT | 3 cut(s) | 272, 348, 947 |
| TaqI | TCGA | 1 cut(s) | 900 |
| TfiI | GAWTC | 3 cut(s) | 485, 605, 982 |
| Tru1I | TTAA | 6 cut(s) | 32, 569, 819, 957, 993, 1212 |
| Tru9I | TTAA | 6 cut(s) | 32, 569, 819, 957, 993, 1212 |
| TscAI | CASTG | 1 cut(s) | 1006 |
| TseFI | GTSAC | 3 cut(s) | 402, 509, 619 |
| TseI | GCWGC | 4 cut(s) | 164, 557, 635, 1024 |
| Tsp45I | GTSAC | 3 cut(s) | 402, 509, 619 |
| TspDTI | ATGAA | 6 cut(s) | 7, 293, 459, 983, 1019, 1256 |
| TspRI | CASTG | 1 cut(s) | 1006 |
| VpaK11BI | GGWCC | 2 cut(s) | 233, 809 |
| XapI | RAATTY | 5 cut(s) | 129, 460, 1190, 1239, 1248 |
| XmnI | GAANNNNTTC | 2 cut(s) | 14, 1065 |