RchiOBHm_Chr5g0057381

Transmembrane amino acid transporter protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0006420
Physical Location & Seq
Reverse (-)
61315379 .. 61316533
1155 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33416

Sequence Viewer

Length: 1053 bp
ATGATGACATGCTATACTGGTATGCTACTAAAAGCATGCATGGATGCGAACCCATCATTAACCACGTACCTCGATATTGTCGAACGAGCATTTGGCAATAAATGGAGGATTATAGCTTCCGTGTTTTTAAACACGGAACTATACTTAGTCGCTCTCAGCTTGATGATAGTCGAAAGTGAAAATTTACACAAATTATTCCCCACGTTTTCCATTAAGCATGGCGGCGGGGCAACAATAATTAGTGGTAGAAAGACTTTTGTCTTAATTACCGCTTGCATTACTTTACCGACAATGCTGCTCACGGATTTGAGCATATTGTCGTACGTGTCGGTGATGGGAGTTCTTTCTTCTCTCCTTATCGTGGGATCGCTCATATCCATCGGAATATCAAAAGTTGGCTTTCACACACAAGGGGGTCAGCTGTGGAACATGAGTGGAATCCCAACCGCTATGAGTTTCTATATTTTCTGCTTCGCGAGCCACTCGGTTCTACCTTCCATTTATGTGTCTATGAGACATCGACAACAGTTCATTACGGTGTTGATGATTAGCTTTCTTCTCACCACCACGACTTGCTTGGTTATAGCTGTGATTGGCTATCTTATGTATGGAGGAGGAGTGCAATCTCAGATAACCCTCAACCTCCCCACCTCAGAAGCAGGTGCGAAAGTCGCTATTTATACATTACTATTAATACCGATTACCCGCTTTGCATTGTTGGTTACCCCTGTAGCTTCGTCTATTGAGGGTCACCTCCCTCAGAAGTACCAAAATAGGAGTTTAATTCAGAGAGCTGTTAGGCTAGTAATTTTGGCTAGCAGTACCTTATTAGCTTATTTCTTTCCCTATTTTGAGACCCTAGTGGGCATACTAGGATCCATTTTTATTGTATTAGCATCTTTTTTATTGCCTTGTGCATGCTACATCAAGCTTACTCATAGTTTTAGTGCATGGAGCTGCAAGTTAGTAGCCACTTTAGCTATAATGGTGTTTGGTGTTCTTGCTGGAGTTCTAGGCACATATACAGCCATTGTTGATCTTGTAAACCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04721 ko04723 ko04727 ko05032 ko05033 map04721 map04723 map04727 map05032 map05033
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

350

Amino Acids

38.13

Weight (kDa)

9.09

Isoelectric Point (pI)

32.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 2 - 344 9.4e-46 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 650
Acc36I ACCTGC 1 cut(s) 650
AccII CGCG 1 cut(s) 476
AciI CCGC 5 cut(s) 222, 225, 270, 447, 706
AclWI GGATC 3 cut(s) 373, 870, 883
AcsI RAATTY 1 cut(s) 181
AfaI GTAC 4 cut(s) 68, 323, 767, 823
AfiI CCNNNNNNNGG 1 cut(s) 361
AflIII ACRYGT 1 cut(s) 324
AjuI GAANNNNNNNTTGG 2 cut(s) 75, 107
Alw26I GTCTC 2 cut(s) 508, 848
AlwI GGATC 3 cut(s) 373, 870, 883
ApeKI GCWGC 2 cut(s) 295, 957
ApoI RAATTY 1 cut(s) 181
AseI ATTAAT 1 cut(s) 692
AsuHPI GGTGA 3 cut(s) 343, 553, 743
AsuNHI GCTAGC 1 cut(s) 815
BamHI GGATCC 1 cut(s) 875
BbvI GCAGC 2 cut(s) 282, 944
BccI CCATC 3 cut(s) 61, 328, 386
BcgI CGANNNNNNTGC 2 cut(s) 277, 311
BcoDI GTCTC 2 cut(s) 508, 848
BfaI CTAG 5 cut(s) 803, 816, 860, 872, 1013
BfmI CTRYAG 1 cut(s) 729
BfuAI ACCTGC 1 cut(s) 650
BisI GCNGC 3 cut(s) 223, 296, 958
BlsI GCNGC 3 cut(s) 224, 297, 959
BmiI GGNNCC 1 cut(s) 877
BmsI GCATC 2 cut(s) 34, 905
BmtI GCTAGC 1 cut(s) 819
BoxI GACNNNNGTC 1 cut(s) 257
BpmI CTGGAG 1 cut(s) 1026
BsaAI YACGTR 2 cut(s) 66, 325
BsaI GGTCTC 1 cut(s) 848
BsaXI ACNNNNNCTCC 2 cut(s) 603, 633
Bsc4I CCNNNNNNNGG 1 cut(s) 361
Bse1I ACTGG 1 cut(s) 22
BseGI GGATG 1 cut(s) 49
BseLI CCNNNNNNNGG 1 cut(s) 361
BseMII CTCAG 4 cut(s) 169, 641, 666, 773
BseNI ACTGG 1 cut(s) 22
BseRI GAGGAG 2 cut(s) 627, 630
BseXI GCAGC 2 cut(s) 282, 944
Bsh1236I CGCG 1 cut(s) 476
BsiWI CGTACG 1 cut(s) 321
BslI CCNNNNNNNGG 1 cut(s) 361
BsmAI GTCTC 2 cut(s) 508, 848
Bso31I GGTCTC 1 cut(s) 848
Bsp143I GATC 3 cut(s) 365, 875, 1036
Bsp68I TCGCGA 1 cut(s) 476
BspACI CCGC 5 cut(s) 222, 225, 270, 447, 706
BspCNI CTCAG 4 cut(s) 168, 640, 665, 772
BspFNI CGCG 1 cut(s) 476
BspLI GGNNCC 1 cut(s) 877
BspMI ACCTGC 1 cut(s) 650
BspOI GCTAGC 1 cut(s) 819
BspPI GGATC 3 cut(s) 373, 870, 883
BspTNI GGTCTC 1 cut(s) 848
BsrI ACTGG 1 cut(s) 22
BssMI GATC 3 cut(s) 365, 875, 1036
Bst4CI ACNGT 2 cut(s) 528, 538
BstBAI YACGTR 2 cut(s) 66, 325
BstC8I GCNNGC 5 cut(s) 37, 274, 478, 817, 919
BstDEI CTNAG 5 cut(s) 145, 155, 627, 652, 759
BstEII GGTNACC 2 cut(s) 721, 749
BstF5I GGATG 1 cut(s) 49
BstFNI CGCG 1 cut(s) 476
BstKTI GATC 3 cut(s) 368, 878, 1039
BstMAI GTCTC 2 cut(s) 508, 848
BstMBI GATC 3 cut(s) 365, 875, 1036
BstMWI GCNNNNNNNGC 3 cut(s) 477, 671, 977
BstNSI RCATGY 3 cut(s) 12, 39, 921
BstPAI GACNNNNGTC 1 cut(s) 257
BstPI GGTNACC 2 cut(s) 721, 749
BstSFI CTRYAG 1 cut(s) 729
BstUI CGCG 1 cut(s) 476
BstV1I GCAGC 2 cut(s) 282, 944
BstX2I RGATCY 1 cut(s) 875
BstYI RGATCY 1 cut(s) 875
BtsCI GGATG 1 cut(s) 49
BtuMI TCGCGA 1 cut(s) 476
BveI ACCTGC 1 cut(s) 650
Cac8I GCNNGC 5 cut(s) 37, 274, 478, 817, 919
Csp6I GTAC 4 cut(s) 67, 322, 766, 822
CviAII CATG 7 cut(s) 9, 36, 40, 218, 430, 918, 951
CviQI GTAC 4 cut(s) 67, 322, 766, 822
DdeI CTNAG 5 cut(s) 145, 155, 627, 652, 759
DpnI GATC 3 cut(s) 367, 877, 1038
DpnII GATC 3 cut(s) 365, 875, 1036
DraI TTTAAA 1 cut(s) 129
Eco31I GGTCTC 1 cut(s) 848
Eco91I GGTNACC 2 cut(s) 721, 749
EcoO65I GGTNACC 2 cut(s) 721, 749
EcoT22I ATGCAT 1 cut(s) 41
FaeI CATG 7 cut(s) 12, 39, 43, 221, 433, 921, 954
FatI CATG 7 cut(s) 8, 35, 39, 217, 429, 917, 950
FauI CCCGC 2 cut(s) 218, 713
Fnu4HI GCNGC 3 cut(s) 223, 296, 958
FokI GGATG 1 cut(s) 56
Fsp4HI GCNGC 3 cut(s) 223, 296, 958
FspBI CTAG 5 cut(s) 803, 816, 860, 872, 1013
GluI GCNGC 3 cut(s) 223, 296, 958
GsuI CTGGAG 1 cut(s) 1026
Hin1II CATG 7 cut(s) 12, 39, 43, 221, 433, 921, 954
HindIII AAGCTT 1 cut(s) 929
HinfI GANTC 1 cut(s) 438
HphI GGTGA 3 cut(s) 343, 553, 743
Hpy166II GTNNAC 1 cut(s) 1045
Hpy188I TCNGA 5 cut(s) 383, 630, 655, 762, 789
Hpy188III TCNNGA 1 cut(s) 475
Hpy8I GTNNAC 1 cut(s) 1045
HpyAV CCTTC 1 cut(s) 504
HpyCH4III ACNGT 2 cut(s) 528, 538
HpyCH4IV ACGT 3 cut(s) 65, 203, 324
HpyCH4V TGCA 7 cut(s) 39, 276, 622, 713, 917, 950, 960
HpyF10VI GCNNNNNNNGC 3 cut(s) 477, 671, 977
HpyF3I CTNAG 5 cut(s) 145, 155, 627, 652, 759
HpySE526I ACGT 3 cut(s) 65, 203, 324
Hsp92II CATG 7 cut(s) 12, 39, 43, 221, 433, 921, 954
Kzo9I GATC 3 cut(s) 365, 875, 1036
LmnI GCTCC 1 cut(s) 954
LpnPI CCDG 4 cut(s) 3, 645, 741, 990
Lsp1109I GCAGC 2 cut(s) 282, 944
LweI GCATC 2 cut(s) 34, 905
MaeI CTAG 5 cut(s) 803, 816, 860, 872, 1013
MaeII ACGT 3 cut(s) 65, 203, 324
MaeIII GTNAC 2 cut(s) 721, 749
MalI GATC 3 cut(s) 367, 877, 1038
MboI GATC 3 cut(s) 365, 875, 1036
MboII GAAGA 2 cut(s) 339, 548
MflI RGATCY 1 cut(s) 875
MluCI AATT 6 cut(s) 181, 191, 237, 264, 783, 807
Mph1103I ATGCAT 1 cut(s) 41
MseI TTAA 7 cut(s) 59, 128, 213, 263, 692, 782, 1051
MslI CAYNNNNRTG 2 cut(s) 503, 536
MspA1I CMGCKG 1 cut(s) 421
MvnI CGCG 1 cut(s) 476
MwoI GCNNNNNNNGC 3 cut(s) 477, 671, 977
NdeII GATC 3 cut(s) 365, 875, 1036
NheI GCTAGC 1 cut(s) 815
NlaIII CATG 7 cut(s) 12, 39, 43, 221, 433, 921, 954
NlaIV GGNNCC 1 cut(s) 877
NmuCI GTSAC 1 cut(s) 749
NruI TCGCGA 1 cut(s) 476
NsiI ATGCAT 1 cut(s) 41
NspI RCATGY 3 cut(s) 12, 39, 921
PaeI GCATGC 2 cut(s) 39, 921
PaqCI CACCTGC 1 cut(s) 650
PcsI WCGNNNNNNNCGW 1 cut(s) 78
PfeI GAWTC 1 cut(s) 438
Pfl23II CGTACG 1 cut(s) 321
PkrI GCNGC 3 cut(s) 224, 297, 959
Ppu21I YACGTR 2 cut(s) 66, 325
PshAI GACNNNNGTC 1 cut(s) 257
PshBI ATTAAT 1 cut(s) 692
PspEI GGTNACC 2 cut(s) 721, 749
PspLI CGTACG 1 cut(s) 321
PspN4I GGNNCC 1 cut(s) 877
PsuI RGATCY 1 cut(s) 875
PvuII CAGCTG 1 cut(s) 421
RruI TCGCGA 1 cut(s) 476
RsaI GTAC 4 cut(s) 68, 323, 767, 823
RsaNI GTAC 4 cut(s) 67, 322, 766, 822
RseI CAYNNNNRTG 2 cut(s) 503, 536
SaqAI TTAA 7 cut(s) 59, 128, 213, 263, 692, 782, 1051
SatI GCNGC 3 cut(s) 223, 296, 958
Sau3AI GATC 3 cut(s) 365, 875, 1036
SfaNI GCATC 2 cut(s) 34, 905
SfcI CTRYAG 1 cut(s) 729
SmiMI CAYNNNNRTG 2 cut(s) 503, 536
SphI GCATGC 2 cut(s) 39, 921
Sse9I AATT 6 cut(s) 181, 191, 237, 264, 783, 807
SsiI CCGC 5 cut(s) 222, 225, 270, 447, 706
SspMI CTAG 5 cut(s) 803, 816, 860, 872, 1013
TaaI ACNGT 2 cut(s) 528, 538
TaiI ACGT 3 cut(s) 68, 206, 327
TaqI TCGA 4 cut(s) 72, 81, 171, 520
TasI AATT 6 cut(s) 181, 191, 237, 264, 783, 807
TauI GCSGC 1 cut(s) 225
TfiI GAWTC 1 cut(s) 438
Tru1I TTAA 7 cut(s) 59, 128, 213, 263, 692, 782, 1051
Tru9I TTAA 7 cut(s) 59, 128, 213, 263, 692, 782, 1051
TseFI GTSAC 1 cut(s) 749
TseI GCWGC 2 cut(s) 295, 957
Tsp45I GTSAC 1 cut(s) 749
TspDTI ATGAA 1 cut(s) 520
TspGWI ACGGA 3 cut(s) 109, 149, 317
VspI ATTAAT 1 cut(s) 692
XapI RAATTY 1 cut(s) 181
XceI RCATGY 3 cut(s) 12, 39, 921
XcmI CCANNNNNNNNNTGG 1 cut(s) 574
XspI CTAG 5 cut(s) 803, 816, 860, 872, 1013
Zsp2I ATGCAT 1 cut(s) 41
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.