Rroxscaffold_1G00025370

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0006420
Physical Location & Seq
Forward (+)
31860173 .. 31862568
2396 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00025370.1

Sequence Viewer

Length: 450 bp
ATGTGGGGATGTGCGATACTGTACGTGATTGTGTATGAAAGGCGTGTTTGCGTTCATACGTCCAAGTCCAGAGACTCTAGGGGAGACGATGACGTCCGGTCCGGTTCCCTTCGAAGACGGACCGATACATTGATGTGGAGGTGTGTGACGGTCAAGGTGGGCCGGTGCGGTGTTACACGGATCGCGGGTCATTGCGGAACGGGTATGCGGTATGGGTACGCGGTACGTCGGTCTCTATGGAACGCGGTTTTTCCTTGTTCTCGTCCGGCCGCGCCGCGACGTGGGTGTAGGCTGCGGCCTCGCCGGCGTGCGTCGAGTGCGGCCGGATGGGATGATGATGTAGTTGAGTGGTACTGTATGAAGCCCGGTCGGATTGAAGGCGGTGCGGTGGTGGCTTTCTTTGTTCGAGTGCTTGCGAGGCCTTGTCGGGGTAAGACGACGGCGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

149

Amino Acids

16.77

Weight (kDa)

10.77

Isoelectric Point (pI)

54.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 92
AatII GACGTC 1 cut(s) 96
AccII CGCG 5 cut(s) 185, 221, 245, 272, 277
AclWI GGATC 1 cut(s) 188
AcoI YGGCCR 2 cut(s) 267, 321
AcyI GRCGYC 1 cut(s) 93
AfaI GTAC 4 cut(s) 23, 218, 225, 353
AfiI CCNNNNNNNGG 2 cut(s) 281, 428
AgsI TTSAA 1 cut(s) 377
AhdI GACNNNNNGTC 1 cut(s) 97
AjiI CACGTC 1 cut(s) 281
Alw26I GTCTC 3 cut(s) 66, 78, 237
AlwI GGATC 1 cut(s) 188
AoxI GGCC 5 cut(s) 160, 267, 296, 321, 419
ApeKI GCWGC 1 cut(s) 292
AspLEI GCGC 1 cut(s) 274
AspS9I GGNCC 3 cut(s) 99, 120, 160
AsuC2I CCSGG 1 cut(s) 366
AsuII TTCGAA 1 cut(s) 112
AvaII GGWCC 2 cut(s) 99, 120
BaeI ACNNNNGTAYC 2 cut(s) 215, 248
BbsI GAAGAC 1 cut(s) 121
BbvI GCAGC 1 cut(s) 279
BccI CCATC 1 cut(s) 321
BcnI CCSGG 1 cut(s) 366
BcoDI GTCTC 3 cut(s) 66, 78, 237
BfaI CTAG 1 cut(s) 78
BglI GCCNNNNNGGC 1 cut(s) 304
BisI GCNGC 6 cut(s) 270, 275, 293, 296, 321, 444
BlsI GCNGC 6 cut(s) 271, 276, 294, 297, 322, 445
Bme1390I CCNGG 1 cut(s) 366
Bme18I GGWCC 2 cut(s) 99, 120
BmeRI GACNNNNNGTC 1 cut(s) 97
BmgBI CACGTC 1 cut(s) 281
BmgT120I GGNCC 3 cut(s) 99, 120, 160
BmiI GGNNCC 1 cut(s) 106
BmrFI CCNGG 1 cut(s) 366
BpiI GAAGAC 1 cut(s) 121
Bpu14I TTCGAA 1 cut(s) 112
BpuMI CCSGG 1 cut(s) 366
BsaAI YACGTR 1 cut(s) 25
BsaHI GRCGYC 1 cut(s) 93
BsaI GGTCTC 1 cut(s) 237
BsaWI WCCGGW 2 cut(s) 96, 101
Bsc4I CCNNNNNNNGG 2 cut(s) 281, 428
Bse118I RCCGGY 2 cut(s) 162, 303
Bse3DI GCAATG 1 cut(s) 190
BseGI GGATG 3 cut(s) 14, 332, 337
BseLI CCNNNNNNNGG 2 cut(s) 281, 428
BseMI GCAATG 1 cut(s) 190
BseX3I CGGCCG 2 cut(s) 267, 321
BseXI GCAGC 1 cut(s) 279
Bsh1236I CGCG 5 cut(s) 185, 221, 245, 272, 277
Bsh1285I CGRYCG 3 cut(s) 270, 324, 370
BshFI GGCC 5 cut(s) 162, 269, 298, 323, 421
BsiEI CGRYCG 3 cut(s) 270, 324, 370
BsiSI CCGG 7 cut(s) 97, 102, 163, 266, 304, 324, 366
BslI CCNNNNNNNGG 2 cut(s) 281, 428
BsmAI GTCTC 3 cut(s) 66, 78, 237
BsmBI CGTCTC 1 cut(s) 78
BsnI GGCC 5 cut(s) 162, 269, 298, 323, 421
Bso31I GGTCTC 1 cut(s) 237
Bsp119I TTCGAA 1 cut(s) 112
Bsp143I GATC 1 cut(s) 180
BspANI GGCC 5 cut(s) 162, 269, 298, 323, 421
BspFNI CGCG 5 cut(s) 185, 221, 245, 272, 277
BspLI GGNNCC 1 cut(s) 106
BspPI GGATC 1 cut(s) 188
BspT104I TTCGAA 1 cut(s) 112
BspTNI GGTCTC 1 cut(s) 237
BsrDI GCAATG 1 cut(s) 190
BsrFI RCCGGY 2 cut(s) 162, 303
BssAI RCCGGY 2 cut(s) 162, 303
BssMI GATC 1 cut(s) 180
BssNI GRCGYC 1 cut(s) 93
Bst4CI ACNGT 3 cut(s) 21, 151, 356
BstACI GRCGYC 1 cut(s) 93
BstBAI YACGTR 1 cut(s) 25
BstBI TTCGAA 1 cut(s) 112
BstC8I GCNNGC 3 cut(s) 305, 309, 414
BstF5I GGATG 3 cut(s) 14, 332, 337
BstFNI CGCG 5 cut(s) 185, 221, 245, 272, 277
BstHHI GCGC 1 cut(s) 274
BstKTI GATC 1 cut(s) 183
BstMAI GTCTC 3 cut(s) 66, 78, 237
BstMBI GATC 1 cut(s) 180
BstMCI CGRYCG 3 cut(s) 270, 324, 370
BstMWI GCNNNNNNNGC 4 cut(s) 304, 317, 392, 418
BstSCI CCNGG 1 cut(s) 364
BstUI CGCG 5 cut(s) 185, 221, 245, 272, 277
BstV1I GCAGC 1 cut(s) 279
BstV2I GAAGAC 1 cut(s) 121
BstZI CGGCCG 2 cut(s) 267, 321
BsuRI GGCC 5 cut(s) 162, 269, 298, 323, 421
BtrI CACGTC 1 cut(s) 281
BtsCI GGATG 3 cut(s) 14, 332, 337
Cac8I GCNNGC 3 cut(s) 305, 309, 414
CfoI GCGC 1 cut(s) 274
Cfr10I RCCGGY 2 cut(s) 162, 303
Cfr13I GGNCC 3 cut(s) 99, 120, 160
CpoI CGGWCCG 2 cut(s) 99, 120
CseI GACGC 1 cut(s) 300
Csp6I GTAC 4 cut(s) 22, 217, 224, 352
CspI CGGWCCG 2 cut(s) 99, 120
CviJI RGCY 9 cut(s) 162, 269, 292, 298, 323, 364, 395, 421, 446
CviKI_1 RGCY 9 cut(s) 162, 269, 292, 298, 323, 364, 395, 421, 446
CviQI GTAC 4 cut(s) 22, 217, 224, 352
DpnI GATC 1 cut(s) 182
DpnII GATC 1 cut(s) 180
DrdI GACNNNNNNGTC 1 cut(s) 92
DriI GACNNNNNGTC 1 cut(s) 97
DseDI GACNNNNNNGTC 1 cut(s) 92
EaeI YGGCCR 2 cut(s) 267, 321
EagI CGGCCG 2 cut(s) 267, 321
Eam1105I GACNNNNNGTC 1 cut(s) 97
EclXI CGGCCG 2 cut(s) 267, 321
Eco147I AGGCCT 1 cut(s) 421
Eco31I GGTCTC 1 cut(s) 237
Eco47I GGWCC 2 cut(s) 99, 120
Eco52I CGGCCG 2 cut(s) 267, 321
Esp3I CGTCTC 1 cut(s) 78
FaiI YATR 6 cut(s) 36, 57, 206, 213, 238, 359
FauI CCCGC 1 cut(s) 178
Fnu4HI GCNGC 6 cut(s) 270, 275, 293, 296, 321, 444
FokI GGATG 3 cut(s) 21, 339, 344
Fsp4HI GCNGC 6 cut(s) 270, 275, 293, 296, 321, 444
FspBI CTAG 1 cut(s) 78
GlaI GCGC 1 cut(s) 273
GluI GCNGC 6 cut(s) 270, 275, 293, 296, 321, 444
HaeIII GGCC 5 cut(s) 162, 269, 298, 323, 421
HapII CCGG 7 cut(s) 97, 102, 163, 266, 304, 324, 366
HgaI GACGC 1 cut(s) 300
HhaI GCGC 1 cut(s) 274
Hin1I GRCGYC 1 cut(s) 93
Hin6I GCGC 1 cut(s) 272
HinP1I GCGC 1 cut(s) 272
HinfI GANTC 1 cut(s) 74
HpaII CCGG 7 cut(s) 97, 102, 163, 266, 304, 324, 366
Hpy188I TCNGA 1 cut(s) 372
Hpy188III TCNNGA 1 cut(s) 69
Hpy99I CGWCG 4 cut(s) 231, 282, 316, 442
HpyAV CCTTC 2 cut(s) 119, 371
HpyCH4III ACNGT 3 cut(s) 21, 151, 356
HpyCH4IV ACGT 5 cut(s) 24, 59, 93, 226, 280
HpyF10VI GCNNNNNNNGC 4 cut(s) 304, 317, 392, 418
HpySE526I ACGT 5 cut(s) 24, 59, 93, 226, 280
Hsp92I GRCGYC 1 cut(s) 93
HspAI GCGC 1 cut(s) 272
KroI GCCGGC 1 cut(s) 303
KroNI GCCGGC 1 cut(s) 305
Kzo9I GATC 1 cut(s) 180
LpnPI CCDG 8 cut(s) 82, 110, 115, 176, 279, 317, 337, 379
Lsp1109I GCAGC 1 cut(s) 279
MaeI CTAG 1 cut(s) 78
MaeII ACGT 5 cut(s) 24, 59, 93, 226, 280
MaeIII GTNAC 2 cut(s) 145, 172
MalI GATC 1 cut(s) 182
MboI GATC 1 cut(s) 180
MboII GAAGA 1 cut(s) 126
MlyI GAGTC 1 cut(s) 68
MmeI TCCRAC 1 cut(s) 350
MnlI CCTC 3 cut(s) 132, 309, 411
MreI CGCCGGCG 1 cut(s) 303
MroNI GCCGGC 1 cut(s) 303
MslI CAYNNNNRTG 1 cut(s) 133
MspI CCGG 7 cut(s) 97, 102, 163, 266, 304, 324, 366
MspR9I CCNGG 1 cut(s) 366
MvnI CGCG 5 cut(s) 185, 221, 245, 272, 277
MwoI GCNNNNNNNGC 4 cut(s) 304, 317, 392, 418
NaeI GCCGGC 1 cut(s) 305
NciI CCSGG 1 cut(s) 366
NdeII GATC 1 cut(s) 180
NgoMIV GCCGGC 1 cut(s) 303
NlaIV GGNNCC 1 cut(s) 106
NmuCI GTSAC 1 cut(s) 145
NspV TTCGAA 1 cut(s) 112
PceI AGGCCT 1 cut(s) 421
PdiI GCCGGC 1 cut(s) 305
PkrI GCNGC 6 cut(s) 271, 276, 294, 297, 322, 445
PleI GAGTC 1 cut(s) 68
PpsI GAGTC 1 cut(s) 68
Ppu21I YACGTR 1 cut(s) 25
PspN4I GGNNCC 1 cut(s) 106
PspPI GGNCC 3 cut(s) 99, 120, 160
RsaI GTAC 4 cut(s) 23, 218, 225, 353
RsaNI GTAC 4 cut(s) 22, 217, 224, 352
RseI CAYNNNNRTG 1 cut(s) 133
Rsr2I CGGWCCG 2 cut(s) 99, 120
RsrII CGGWCCG 2 cut(s) 99, 120
SatI GCNGC 6 cut(s) 270, 275, 293, 296, 321, 444
Sau3AI GATC 1 cut(s) 180
Sau96I GGNCC 3 cut(s) 99, 120, 160
SchI GAGTC 1 cut(s) 68
ScrFI CCNGG 1 cut(s) 366
SetI ASST 7 cut(s) 27, 62, 96, 143, 159, 229, 283
SfuI TTCGAA 1 cut(s) 112
SgrAI CRCCGGYG 1 cut(s) 303
SinI GGWCC 2 cut(s) 99, 120
SmiMI CAYNNNNRTG 1 cut(s) 133
SseBI AGGCCT 1 cut(s) 421
SspMI CTAG 1 cut(s) 78
StuI AGGCCT 1 cut(s) 421
StyD4I CCNGG 1 cut(s) 364
TaaI ACNGT 3 cut(s) 21, 151, 356
TaiI ACGT 5 cut(s) 27, 62, 96, 229, 283
TaqI TCGA 3 cut(s) 112, 314, 406
TaqII GACCGA 2 cut(s) 137, 219
TauI GCSGC 5 cut(s) 272, 277, 298, 323, 446
TseFI GTSAC 1 cut(s) 145
TseI GCWGC 1 cut(s) 292
Tsp45I GTSAC 1 cut(s) 145
TspDTI ATGAA 3 cut(s) 44, 51, 374
TspGWI ACGGA 2 cut(s) 133, 193
VpaK11BI GGWCC 2 cut(s) 99, 120
XspI CTAG 1 cut(s) 78
ZraI GACGTC 1 cut(s) 94
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.