Rh5DG400200

Transmembrane amino acid transporter protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0006420
Physical Location & Seq
Reverse (-)
63059065 .. 63061334
2270 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG400200.1

Sequence Viewer

Length: 657 bp
ATGAACAATGAAAGTATTACTCCTTCGACGGGCCAATCCAGCCTTTGGAAAGCTACGTTTAATACAACTAGTGTGTTTTTAGGCCACCCGGTTCTACCTTCCATTTATGTGTCTATGAGACATCGACAACAGTTCATTAAGGTGTTGATGATTAGCTTTCTTCTCACCACCAAGACTTGCTTGGTTACATCTATGATTGGCTATCTTATGTATGGAGGAGGAGTGCAATCTCAGATAACCCTCAACCTCCCCACCTCAGAAGCAGGTGCGAAAGTCGCTATTTATACATTACTATTAATACCGATTACCCGCTTTGCATTGTTGGTTACCCCTGTAGCTTCGTCTATTGAGGGTCACCTCCCTCAGAAGTACCAAAATAGGAGTTTAATTCAGAGAGCTGTTAGGCTAGTAATTTTGGCTAGCAGTACCTTATTAGCTTATTTCTTTCCCTATTTTGAGACCATAGTGGGCATACTAGGATCCATTTTTATTGTATTAGCATCTTTTTTACTGCCTTGTGCATGCTACATCAAGCTTACTCATAGCTTTAGTACATGGAGCTGCAAGTTAGTAGCCACTTTAGCTATTTTGGTGTTTGGTGTTCTTTCCGGAGTTCTAGGCACATATACAGCCATTGTTGATCTTGTAAACCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04721 ko04723 ko04727 ko05032 ko05033 map04721 map04723 map04727 map05032 map05033
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

218

Amino Acids

23.86

Weight (kDa)

9.51

Isoelectric Point (pI)

34.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 26 - 212 3.9e-27 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 254
Acc36I ACCTGC 1 cut(s) 254
AccB7I CCANNNNNTGG 1 cut(s) 45
AccIII TCCGGA 1 cut(s) 608
AciI CCGC 1 cut(s) 310
AclWI GGATC 2 cut(s) 474, 487
AfaI GTAC 3 cut(s) 371, 427, 553
AfiI CCNNNNNNNGG 2 cut(s) 29, 45
AhlI ACTAGT 1 cut(s) 68
AluBI AGCT 9 cut(s) 53, 156, 338, 398, 437, 535, 546, 561, 584
AluI AGCT 9 cut(s) 53, 156, 338, 398, 437, 535, 546, 561, 584
Alw26I GTCTC 2 cut(s) 112, 452
AlwI GGATC 2 cut(s) 474, 487
Aor13HI TCCGGA 1 cut(s) 608
AoxI GGCC 2 cut(s) 31, 82
ApeKI GCWGC 1 cut(s) 561
AseI ATTAAT 1 cut(s) 296
AspS9I GGNCC 1 cut(s) 31
AsuC2I CCSGG 1 cut(s) 89
AsuHPI GGTGA 2 cut(s) 157, 347
AsuNHI GCTAGC 1 cut(s) 419
BamHI GGATCC 1 cut(s) 479
BarI GAAGNNNNNNTAC 2 cut(s) 7, 39
BbvI GCAGC 1 cut(s) 548
BcnI CCSGG 1 cut(s) 89
BcoDI GTCTC 2 cut(s) 112, 452
BcuI ACTAGT 1 cut(s) 68
BfaI CTAG 5 cut(s) 69, 407, 420, 476, 617
BfmI CTRYAG 1 cut(s) 333
BfuAI ACCTGC 1 cut(s) 254
BisI GCNGC 1 cut(s) 562
BlsI GCNGC 1 cut(s) 563
Bme1390I CCNGG 1 cut(s) 89
BmgT120I GGNCC 1 cut(s) 31
BmiI GGNNCC 1 cut(s) 481
BmrFI CCNGG 1 cut(s) 89
BmsI GCATC 1 cut(s) 509
BmtI GCTAGC 1 cut(s) 423
BpuMI CCSGG 1 cut(s) 89
BsaI GGTCTC 1 cut(s) 452
BsaWI WCCGGW 1 cut(s) 608
BsaXI ACNNNNNCTCC 2 cut(s) 207, 237
Bsc4I CCNNNNNNNGG 2 cut(s) 29, 45
BseAI TCCGGA 1 cut(s) 608
BseLI CCNNNNNNNGG 2 cut(s) 29, 45
BseMII CTCAG 3 cut(s) 245, 270, 377
BseRI GAGGAG 2 cut(s) 231, 234
BseXI GCAGC 1 cut(s) 548
BshFI GGCC 2 cut(s) 33, 84
BsiSI CCGG 2 cut(s) 89, 609
BslI CCNNNNNNNGG 2 cut(s) 29, 45
BsmAI GTCTC 2 cut(s) 112, 452
BsnI GGCC 2 cut(s) 33, 84
Bso31I GGTCTC 1 cut(s) 452
Bsp13I TCCGGA 1 cut(s) 608
Bsp143I GATC 2 cut(s) 479, 640
BspACI CCGC 1 cut(s) 310
BspANI GGCC 2 cut(s) 33, 84
BspCNI CTCAG 3 cut(s) 244, 269, 376
BspEI TCCGGA 1 cut(s) 608
BspLI GGNNCC 1 cut(s) 481
BspMI ACCTGC 1 cut(s) 254
BspOI GCTAGC 1 cut(s) 423
BspPI GGATC 2 cut(s) 474, 487
BspTNI GGTCTC 1 cut(s) 452
BssMI GATC 2 cut(s) 479, 640
Bst4CI ACNGT 1 cut(s) 132
BstC8I GCNNGC 2 cut(s) 421, 523
BstDEI CTNAG 3 cut(s) 231, 256, 363
BstEII GGTNACC 2 cut(s) 325, 353
BstKTI GATC 2 cut(s) 482, 643
BstMAI GTCTC 2 cut(s) 112, 452
BstMBI GATC 2 cut(s) 479, 640
BstMWI GCNNNNNNNGC 3 cut(s) 39, 275, 581
BstNSI RCATGY 1 cut(s) 525
BstPI GGTNACC 2 cut(s) 325, 353
BstSCI CCNGG 1 cut(s) 87
BstSFI CTRYAG 1 cut(s) 333
BstV1I GCAGC 1 cut(s) 548
BstX2I RGATCY 1 cut(s) 479
BstYI RGATCY 1 cut(s) 479
BsuRI GGCC 2 cut(s) 33, 84
BveI ACCTGC 1 cut(s) 254
Cac8I GCNNGC 2 cut(s) 421, 523
Cfr13I GGNCC 1 cut(s) 31
Csp6I GTAC 3 cut(s) 370, 426, 552
CviAII CATG 2 cut(s) 522, 555
CviQI GTAC 3 cut(s) 370, 426, 552
DdeI CTNAG 3 cut(s) 231, 256, 363
DpnI GATC 2 cut(s) 481, 642
DpnII GATC 2 cut(s) 479, 640
Eco31I GGTCTC 1 cut(s) 452
Eco91I GGTNACC 2 cut(s) 325, 353
EcoO65I GGTNACC 2 cut(s) 325, 353
FaeI CATG 2 cut(s) 525, 558
FalI AAGNNNNNCTT 2 cut(s) 164, 196
FatI CATG 2 cut(s) 521, 554
FauI CCCGC 1 cut(s) 317
Fnu4HI GCNGC 1 cut(s) 562
Fsp4HI GCNGC 1 cut(s) 562
FspBI CTAG 5 cut(s) 69, 407, 420, 476, 617
GluI GCNGC 1 cut(s) 562
HaeIII GGCC 2 cut(s) 33, 84
HapII CCGG 2 cut(s) 89, 609
Hin1II CATG 2 cut(s) 525, 558
HindIII AAGCTT 1 cut(s) 533
HpaII CCGG 2 cut(s) 89, 609
HphI GGTGA 2 cut(s) 157, 347
Hpy166II GTNNAC 1 cut(s) 649
Hpy188I TCNGA 4 cut(s) 234, 259, 366, 393
Hpy188III TCNNGA 1 cut(s) 609
Hpy8I GTNNAC 1 cut(s) 649
Hpy99I CGWCG 1 cut(s) 31
HpyAV CCTTC 2 cut(s) 33, 108
HpyCH4III ACNGT 1 cut(s) 132
HpyCH4IV ACGT 1 cut(s) 56
HpyCH4V TGCA 4 cut(s) 226, 317, 521, 564
HpyF10VI GCNNNNNNNGC 3 cut(s) 39, 275, 581
HpyF3I CTNAG 3 cut(s) 231, 256, 363
HpySE526I ACGT 1 cut(s) 56
Hsp92II CATG 2 cut(s) 525, 558
Kpn2I TCCGGA 1 cut(s) 608
Kzo9I GATC 2 cut(s) 479, 640
LmnI GCTCC 1 cut(s) 558
LpnPI CCDG 5 cut(s) 52, 102, 249, 345, 622
Lsp1109I GCAGC 1 cut(s) 548
LweI GCATC 1 cut(s) 509
MaeI CTAG 5 cut(s) 69, 407, 420, 476, 617
MaeII ACGT 1 cut(s) 56
MaeIII GTNAC 3 cut(s) 184, 325, 353
MalI GATC 2 cut(s) 481, 642
MboI GATC 2 cut(s) 479, 640
MboII GAAGA 1 cut(s) 152
MflI RGATCY 1 cut(s) 479
MluCI AATT 2 cut(s) 387, 411
MnlI CCTC 8 cut(s) 209, 212, 251, 257, 265, 343, 368, 372
MroI TCCGGA 1 cut(s) 608
MseI TTAA 5 cut(s) 60, 138, 296, 386, 655
MslI CAYNNNNRTG 2 cut(s) 107, 140
MspI CCGG 2 cut(s) 89, 609
MspR9I CCNGG 1 cut(s) 89
MwoI GCNNNNNNNGC 3 cut(s) 39, 275, 581
NciI CCSGG 1 cut(s) 89
NdeII GATC 2 cut(s) 479, 640
NheI GCTAGC 1 cut(s) 419
NlaIII CATG 2 cut(s) 525, 558
NlaIV GGNNCC 1 cut(s) 481
NmuCI GTSAC 1 cut(s) 353
NspI RCATGY 1 cut(s) 525
PaeI GCATGC 1 cut(s) 525
PaqCI CACCTGC 1 cut(s) 254
PflMI CCANNNNNTGG 1 cut(s) 45
PkrI GCNGC 1 cut(s) 563
PshBI ATTAAT 1 cut(s) 296
PspEI GGTNACC 2 cut(s) 325, 353
PspN4I GGNNCC 1 cut(s) 481
PspPI GGNCC 1 cut(s) 31
PsuI RGATCY 1 cut(s) 479
RsaI GTAC 3 cut(s) 371, 427, 553
RsaNI GTAC 3 cut(s) 370, 426, 552
RseI CAYNNNNRTG 2 cut(s) 107, 140
SaqAI TTAA 5 cut(s) 60, 138, 296, 386, 655
SatI GCNGC 1 cut(s) 562
Sau3AI GATC 2 cut(s) 479, 640
Sau96I GGNCC 1 cut(s) 31
ScrFI CCNGG 1 cut(s) 89
SfaNI GCATC 1 cut(s) 509
SfcI CTRYAG 1 cut(s) 333
SmiMI CAYNNNNRTG 2 cut(s) 107, 140
SpeI ACTAGT 1 cut(s) 68
SphI GCATGC 1 cut(s) 525
Sse9I AATT 2 cut(s) 387, 411
SsiI CCGC 1 cut(s) 310
SspMI CTAG 5 cut(s) 69, 407, 420, 476, 617
StyD4I CCNGG 1 cut(s) 87
TaaI ACNGT 1 cut(s) 132
TaiI ACGT 1 cut(s) 59
TaqI TCGA 2 cut(s) 26, 124
TasI AATT 2 cut(s) 387, 411
TatI WGTACW 1 cut(s) 551
Tru1I TTAA 5 cut(s) 60, 138, 296, 386, 655
Tru9I TTAA 5 cut(s) 60, 138, 296, 386, 655
TseFI GTSAC 1 cut(s) 353
TseI GCWGC 1 cut(s) 561
Tsp45I GTSAC 1 cut(s) 353
TspDTI ATGAA 3 cut(s) 17, 24, 124
Van91I CCANNNNNTGG 1 cut(s) 45
VspI ATTAAT 1 cut(s) 296
XceI RCATGY 1 cut(s) 525
XcmI CCANNNNNNNNNTGG 1 cut(s) 178
XspI CTAG 5 cut(s) 69, 407, 420, 476, 617
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.