Rw5G035350

Vacuolar amino acid transporter 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0006420
Physical Location & Seq
Reverse (-)
61121478 .. 61123495
2018 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G035350.1

Sequence Viewer

Length: 1212 bp
ATGAACAGTGAAAGTACTACTCCTCTGAAGGCCCAATCCAGCCATTGGAAAGCCACCTTTAATACAACTAGTGTGCTTTTAGGGATAGGCATTGTTACGCTACCGTACGCACTCTCAAGAGGAGGGTGGGTCTCGCTTCTTCTTTGCGTTGTCATTGCCATGATGACATGTTATACTAGCATGCTACTTAAAACTTGCATGGATGCGGACCCATCAATAAACACCTACCTCGATATTATCGAACGAGCTTTTGGCAATAAATGGAGGATTATAGCTTCTGTGTTTTTAAACACAGAACTATACTTAGTTGCTCTTGGCTTGGTAATAGCCGAAAGTGACAATTTACAAAAATTATTCCCCACATTTGCCATTAAGCTTGGCGGCGGGGCAATAACAATTGGCTGTAGAAAGACTTTCGTCTTAATTACGGCTTGTATTACTTTACCGACAATGCTACTCACTGATTTGAGCATATTGTCGTATGTGTCAGCCATGGGAGTTCTTTCTTCCCTCCTCATCATGGGATCACTCATATATGTTGGACTATCAGGAGTTGGCTTTCACGCACAAGGAGGTCAGCTGTGGAACATGAGTGGACTCCCGACAGCTATGAGTTTCTACATTTTCTGCTTCGCGGGCCACCCAGTTCTACCTTCCATTTATATGTCTATGAGACATCGACATCAGTTCATTAAGGTGTTGATGATCAGCTTTCTTCTCACCACTACAACTTGCTTGGTTACAGCTGTGATTGGTTATCTTATGTACGGAGGAGAAGTGAAATCTCAGATAACCCTCAACCTCCCCACCTCACAAGCCAGTGCGAAAATAGCTATTTATTCATTACTATTAATACCAATTACTCGCTATGCACTGATGGTTACCCCAGTAGCTTCAGCTATTGAAGGTCTCCTGCCTCAGAACTACCAAAATAGGAGTTTAATTCAGAGAGTTATTAGGCTAGTAATTTTGGCTAGCAATACCTTATTAGCCTTTTTCTTTCCCTATTTTGAGACCCTAATGGGCTTACTGGGATCCATTTTTGTTGTCTCAGCATCTTTTTTACTGCCTTGTGCATGCTACATCAAGCTTACTCATGGCTTTAGTACTTGGAGTTGCAAGTTAGTAGTTGCTGTAGCTATAATGGTGTTTGGTGTTCTTGCCGGAATTCTAGGCACATATACAGCCATTGTTGATCTTGTAAGCCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04721 ko04723 ko04727 ko05032 ko05033 map04721 map04723 map04727 map05032 map05033
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

403

Amino Acids

43.79

Weight (kDa)

9.04

Isoelectric Point (pI)

34.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 12 - 397 2.9e-62 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 45
AccII CGCG 1 cut(s) 635
AciI CCGC 4 cut(s) 206, 381, 384, 635
AclWI GGATC 3 cut(s) 532, 1029, 1042
AcsI RAATTY 1 cut(s) 1167
AcuI CTGAAG 2 cut(s) 47, 879
AfaI GTAC 4 cut(s) 16, 107, 767, 1108
AfiI CCNNNNNNNGG 2 cut(s) 45, 520
AflIII ACRYGT 1 cut(s) 167
AgsI TTSAA 1 cut(s) 905
AhlI ACTAGT 1 cut(s) 68
AjuI GAANNNNNNNTTGG 2 cut(s) 234, 266
Alw26I GTCTC 5 cut(s) 136, 667, 914, 1007, 1054
AlwI GGATC 3 cut(s) 532, 1029, 1042
AoxI GGCC 2 cut(s) 30, 637
ApoI RAATTY 1 cut(s) 1167
AseI ATTAAT 1 cut(s) 851
AspS9I GGNCC 3 cut(s) 31, 208, 637
AsuHPI GGTGA 1 cut(s) 712
AsuNHI GCTAGC 1 cut(s) 974
AvaII GGWCC 1 cut(s) 208
BamHI GGATCC 1 cut(s) 1034
BccI CCATC 2 cut(s) 220, 871
BceAI ACGGC 1 cut(s) 444
BclI TGATCA 1 cut(s) 705
BcoDI GTCTC 5 cut(s) 136, 667, 914, 1007, 1054
BcuI ACTAGT 1 cut(s) 68
BfaI CTAG 5 cut(s) 69, 177, 962, 975, 1172
BfmI CTRYAG 2 cut(s) 403, 1134
BisI GCNGC 1 cut(s) 382
BlsI GCNGC 1 cut(s) 383
BmcAI AGTACT 2 cut(s) 16, 1108
Bme18I GGWCC 1 cut(s) 208
BmgT120I GGNCC 3 cut(s) 31, 208, 637
BmiI GGNNCC 2 cut(s) 210, 1036
BmrI ACTGGG 3 cut(s) 638, 881, 1040
BmsI GCATC 2 cut(s) 193, 1064
BmtI GCTAGC 1 cut(s) 978
BmuI ACTGGG 3 cut(s) 638, 881, 1040
BoxI GACNNNNGTC 1 cut(s) 416
BpuEI CTTGAG 1 cut(s) 100
BsaI GGTCTC 3 cut(s) 136, 914, 1007
BsaJI CCNNGG 1 cut(s) 492
BsaXI ACNNNNNCTCC 4 cut(s) 114, 144, 762, 792
Bsc4I CCNNNNNNNGG 2 cut(s) 45, 520
Bse1I ACTGG 4 cut(s) 644, 819, 887, 1035
Bse3DI GCAATG 1 cut(s) 153
BseDI CCNNGG 1 cut(s) 492
BseGI GGATG 1 cut(s) 208
BseLI CCNNNNNNNGG 2 cut(s) 45, 520
BseMI GCAATG 1 cut(s) 153
BseMII CTCAG 3 cut(s) 800, 932, 1065
BseNI ACTGG 4 cut(s) 644, 819, 887, 1035
BseRI GAGGAG 4 cut(s) 12, 135, 503, 786
Bsh1236I CGCG 1 cut(s) 635
BshFI GGCC 2 cut(s) 32, 639
BsiSI CCGG 1 cut(s) 1164
BsiWI CGTACG 1 cut(s) 105
BslI CCNNNNNNNGG 2 cut(s) 45, 520
BsmAI GTCTC 5 cut(s) 136, 667, 914, 1007, 1054
BsnI GGCC 2 cut(s) 32, 639
Bso31I GGTCTC 3 cut(s) 136, 914, 1007
Bsp143I GATC 4 cut(s) 524, 705, 1034, 1195
Bsp19I CCATGG 1 cut(s) 492
BspACI CCGC 4 cut(s) 206, 381, 384, 635
BspANI GGCC 2 cut(s) 32, 639
BspCNI CTCAG 3 cut(s) 799, 931, 1064
BspFNI CGCG 1 cut(s) 635
BspLI GGNNCC 2 cut(s) 210, 1036
BspOI GCTAGC 1 cut(s) 978
BspPI GGATC 3 cut(s) 532, 1029, 1042
BspTNI GGTCTC 3 cut(s) 136, 914, 1007
BsrDI GCAATG 1 cut(s) 153
BsrI ACTGG 4 cut(s) 644, 819, 887, 1035
BssECI CCNNGG 1 cut(s) 492
BssMI GATC 4 cut(s) 524, 705, 1034, 1195
BssT1I CCWWGG 1 cut(s) 492
Bst4CI ACNGT 2 cut(s) 8, 105
BstC8I GCNNGC 4 cut(s) 182, 637, 976, 1078
BstDEI CTNAG 4 cut(s) 304, 786, 918, 1051
BstDSI CCRYGG 1 cut(s) 492
BstEII GGTNACC 1 cut(s) 880
BstF5I GGATG 1 cut(s) 208
BstFNI CGCG 1 cut(s) 635
BstKTI GATC 4 cut(s) 527, 708, 1037, 1198
BstMAI GTCTC 5 cut(s) 136, 667, 914, 1007, 1054
BstMBI GATC 4 cut(s) 524, 705, 1034, 1195
BstMWI GCNNNNNNNGC 2 cut(s) 636, 830
BstNSI RCATGY 3 cut(s) 171, 184, 1080
BstPAI GACNNNNGTC 1 cut(s) 416
BstPI GGTNACC 1 cut(s) 880
BstSFI CTRYAG 2 cut(s) 403, 1134
BstUI CGCG 1 cut(s) 635
BstX2I RGATCY 1 cut(s) 1034
BstYI RGATCY 1 cut(s) 1034
BsuRI GGCC 2 cut(s) 32, 639
BtgI CCRYGG 1 cut(s) 492
BtsCI GGATG 1 cut(s) 208
BtsIMutI CAGTG 4 cut(s) 13, 459, 826, 872
Cac8I GCNNGC 4 cut(s) 182, 637, 976, 1078
Cfr13I GGNCC 3 cut(s) 31, 208, 637
Csp6I GTAC 4 cut(s) 15, 106, 766, 1107
CviAII CATG 9 cut(s) 160, 168, 181, 199, 493, 520, 589, 1077, 1097
CviQI GTAC 4 cut(s) 15, 106, 766, 1107
DdeI CTNAG 4 cut(s) 304, 786, 918, 1051
DpnI GATC 4 cut(s) 526, 707, 1036, 1197
DpnII GATC 4 cut(s) 524, 705, 1034, 1195
DraI TTTAAA 1 cut(s) 288
Eco130I CCWWGG 1 cut(s) 492
Eco31I GGTCTC 3 cut(s) 136, 914, 1007
Eco47I GGWCC 1 cut(s) 208
Eco57I CTGAAG 2 cut(s) 47, 879
Eco91I GGTNACC 1 cut(s) 880
EcoO65I GGTNACC 1 cut(s) 880
EcoRI GAATTC 1 cut(s) 1167
EcoT14I CCWWGG 1 cut(s) 492
ErhI CCWWGG 1 cut(s) 492
FaeI CATG 9 cut(s) 163, 171, 184, 202, 496, 523, 592, 1080, 1100
FatI CATG 9 cut(s) 159, 167, 180, 198, 492, 519, 588, 1076, 1096
FauI CCCGC 2 cut(s) 377, 628
FbaI TGATCA 1 cut(s) 705
Fnu4HI GCNGC 1 cut(s) 382
FokI GGATG 1 cut(s) 215
Fsp4HI GCNGC 1 cut(s) 382
FspBI CTAG 5 cut(s) 69, 177, 962, 975, 1172
GluI GCNGC 1 cut(s) 382
HaeIII GGCC 2 cut(s) 32, 639
HapII CCGG 1 cut(s) 1164
Hin1II CATG 9 cut(s) 163, 171, 184, 202, 496, 523, 592, 1080, 1100
HindIII AAGCTT 2 cut(s) 374, 1088
HinfI GANTC 1 cut(s) 597
HpaII CCGG 1 cut(s) 1164
HphI GGTGA 1 cut(s) 712
Hpy166II GTNNAC 1 cut(s) 596
Hpy188I TCNGA 4 cut(s) 27, 789, 921, 948
Hpy188III TCNNGA 3 cut(s) 117, 549, 601
Hpy8I GTNNAC 1 cut(s) 596
HpyAV CCTTC 3 cut(s) 22, 663, 899
HpyCH4III ACNGT 2 cut(s) 8, 105
HpyCH4V TGCA 4 cut(s) 198, 872, 1076, 1119
HpyF10VI GCNNNNNNNGC 2 cut(s) 636, 830
HpyF3I CTNAG 4 cut(s) 304, 786, 918, 1051
Hsp92II CATG 9 cut(s) 163, 171, 184, 202, 496, 523, 592, 1080, 1100
Ksp22I TGATCA 1 cut(s) 705
Kzo9I GATC 4 cut(s) 524, 705, 1034, 1195
LpnPI CCDG 8 cut(s) 52, 534, 657, 832, 900, 926, 1016, 1177
LweI GCATC 2 cut(s) 193, 1064
MaeI CTAG 5 cut(s) 69, 177, 962, 975, 1172
MaeIII GTNAC 4 cut(s) 94, 335, 739, 880
MalI GATC 4 cut(s) 526, 707, 1036, 1197
MboI GATC 4 cut(s) 524, 705, 1034, 1195
MboII GAAGA 3 cut(s) 131, 498, 707
MfeI CAATTG 1 cut(s) 396
MflI RGATCY 1 cut(s) 1034
MluCI AATT 8 cut(s) 340, 350, 396, 423, 858, 942, 966, 1167
MlyI GAGTC 1 cut(s) 591
MmeI TCCRAC 1 cut(s) 520
MseI TTAA 9 cut(s) 60, 189, 287, 372, 422, 693, 851, 941, 1210
MslI CAYNNNNRTG 3 cut(s) 158, 662, 695
MspA1I CMGCKG 2 cut(s) 580, 746
MspI CCGG 1 cut(s) 1164
MunI CAATTG 1 cut(s) 396
MvnI CGCG 1 cut(s) 635
MwoI GCNNNNNNNGC 2 cut(s) 636, 830
NcoI CCATGG 1 cut(s) 492
NdeII GATC 4 cut(s) 524, 705, 1034, 1195
NheI GCTAGC 1 cut(s) 974
NlaIII CATG 9 cut(s) 163, 171, 184, 202, 496, 523, 592, 1080, 1100
NlaIV GGNNCC 2 cut(s) 210, 1036
NmuCI GTSAC 1 cut(s) 335
NspI RCATGY 3 cut(s) 171, 184, 1080
PaeI GCATGC 2 cut(s) 184, 1080
PciI ACATGT 1 cut(s) 167
PcsI WCGNNNNNNNCGW 1 cut(s) 237
Pfl23II CGTACG 1 cut(s) 105
PflMI CCANNNNNTGG 1 cut(s) 45
PkrI GCNGC 1 cut(s) 383
PleI GAGTC 1 cut(s) 591
PpsI GAGTC 1 cut(s) 591
PscI ACATGT 1 cut(s) 167
PshAI GACNNNNGTC 1 cut(s) 416
PshBI ATTAAT 1 cut(s) 851
PspEI GGTNACC 1 cut(s) 880
PspLI CGTACG 1 cut(s) 105
PspN4I GGNNCC 2 cut(s) 210, 1036
PspPI GGNCC 3 cut(s) 31, 208, 637
PsuI RGATCY 1 cut(s) 1034
PvuII CAGCTG 2 cut(s) 580, 746
RsaI GTAC 4 cut(s) 16, 107, 767, 1108
RsaNI GTAC 4 cut(s) 15, 106, 766, 1107
RseI CAYNNNNRTG 3 cut(s) 158, 662, 695
SaqAI TTAA 9 cut(s) 60, 189, 287, 372, 422, 693, 851, 941, 1210
SatI GCNGC 1 cut(s) 382
Sau3AI GATC 4 cut(s) 524, 705, 1034, 1195
Sau96I GGNCC 3 cut(s) 31, 208, 637
ScaI AGTACT 2 cut(s) 16, 1108
SchI GAGTC 1 cut(s) 591
SfaNI GCATC 2 cut(s) 193, 1064
SfcI CTRYAG 2 cut(s) 403, 1134
SinI GGWCC 1 cut(s) 208
SmiMI CAYNNNNRTG 3 cut(s) 158, 662, 695
SmlI CTYRAG 1 cut(s) 115
SmoI CTYRAG 1 cut(s) 115
SpeI ACTAGT 1 cut(s) 68
SphI GCATGC 2 cut(s) 184, 1080
Sse9I AATT 8 cut(s) 340, 350, 396, 423, 858, 942, 966, 1167
SsiI CCGC 4 cut(s) 206, 381, 384, 635
SspMI CTAG 5 cut(s) 69, 177, 962, 975, 1172
StyI CCWWGG 1 cut(s) 492
TaaI ACNGT 2 cut(s) 8, 105
TaqI TCGA 3 cut(s) 231, 240, 679
TasI AATT 8 cut(s) 340, 350, 396, 423, 858, 942, 966, 1167
TatI WGTACW 2 cut(s) 14, 1106
TauI GCSGC 1 cut(s) 384
Tru1I TTAA 9 cut(s) 60, 189, 287, 372, 422, 693, 851, 941, 1210
Tru9I TTAA 9 cut(s) 60, 189, 287, 372, 422, 693, 851, 941, 1210
TscAI CASTG 4 cut(s) 13, 466, 826, 879
TseFI GTSAC 1 cut(s) 335
Tsp45I GTSAC 1 cut(s) 335
TspDTI ATGAA 3 cut(s) 17, 679, 831
TspGWI ACGGA 1 cut(s) 783
TspRI CASTG 4 cut(s) 13, 466, 826, 879
Van91I CCANNNNNTGG 1 cut(s) 45
VpaK11BI GGWCC 1 cut(s) 208
VspI ATTAAT 1 cut(s) 851
XapI RAATTY 1 cut(s) 1167
XceI RCATGY 3 cut(s) 171, 184, 1080
XspI CTAG 5 cut(s) 69, 177, 962, 975, 1172
ZrmI AGTACT 2 cut(s) 16, 1108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.