Rroxscaffold_1G00053300

Vacuolar amino acid transporter 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0006420
Physical Location & Seq
Forward (+)
74135473 .. 74138503
3031 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00053300.1

Sequence Viewer

Length: 1263 bp
ATGGCAAGTATCGACTCAAGGACTCCGCTGCTAACTGGAATTAGCACAGGGTCTTGCAGCTTCTTCATGGCTTGTTTTAATGGAACAAGTGCATTTTTAGGTATTGGTCTTATAGCTATTCCTTACGCCCTCTCAAAAGCAGGATGGCTAAGCTTAGTGTTATGCCTACTAATTGCCATTATGACCTTTTACACTGCCTTCTTGCTCAAGAAATGCATGGATTCCGACCAGTCAATCACAAGTTACCTTGACATTGCCGAGCGCGCATTTGATAAAAGAGCCAGAACCATAGCTTCAATTTTCTTGAACACAGAAATGTACCTGGTCGCCACAGGGCTCATAATATCAGAAAGTGACAACTTACATAAGTTGTTTCCAGACTTCACGGTGGATTTCGGGCCATTGGTAATTGGTGGTAGGCAGTCGTTTGTGTTAATTACAGCATTGATTATATCTCCAACGATGATGATGATTGATTTGAGTGTATTGTCATACGTCTCGGCTACTGGGGTGTTTTCCACCCTTGTCATTGTTGTCTCCCTTGTGTGTGTCGGAGCATTTGGAGGTGTTGGGTTTCAAGGCAGTGGAGTTTTGTTAAATGTGAGTGGAATTTCTACTGCCGTGAGCTTGTATATCTTTTGCTTTGCAGGACATCCAGTCATCCCTTCAATATACAAATCTATGCGAAACAGGGATCAGTTCGGTAAGGTCTTGTTCGTTATCTACTTCCTCACAACTATCACTTGCATGGTAACGGCTGTTATAGGCTATTTAATGTTCGGAAATAACACCGAATCTCAAATCACTTTGAATCTACCAATTACAGAAATCAGTGCACAAATTACAATCTACACCGTTCTACTTATTCCAGTAACAAGATATGCATTGCTTGCAACCCCAGTTGCTAATGTCATTGAAGGTGGAGTATTATCAAAAGATGGCAACAATTCGAGACTGCTTGCACGGCTTATAAGGTTGGGATTACTAGTAAGCTCTACTATGGTAGCCTACGTTTTCCCTTACTTTGAGAACCTCATAAGCATTAAAACTTTGTCTAGGTTTTTCCTTGTTCTCGTCCGGCTGCGCCGCGACGTGGGTGTAGGCTGCGGCCTCGCCGGCGTGCGTCGAGTGCGGCCGGATGGGATGATGATGTCGTTGAGTGGTACTTGTATGAAGCCCGGTCGGATTGAAGGCGGTGCGGTGGTGGCTTTGCTGTTCCGAGTGCTTGCGAGGCCTTGTCGGGGTAAGACGGCGGCGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04721 ko04723 ko04727 ko05032 ko05033 map04721 map04723 map04727 map05032 map05033
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

420

Amino Acids

45.28

Weight (kDa)

9.32

Isoelectric Point (pI)

32.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 19 - 348 7.6e-53 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 971
AccII CGCG 2 cut(s) 264, 1089
AciI CCGC 8 cut(s) 26, 1087, 1107, 1132, 1194, 1199, 1253, 1256
AclWI GGATC 1 cut(s) 702
AcoI YGGCCR 1 cut(s) 1133
AcsI RAATTY 1 cut(s) 609
AfaI GTAC 2 cut(s) 320, 1165
AfiI CCNNNNNNNGG 2 cut(s) 1093, 1241
AgsI TTSAA 7 cut(s) 297, 307, 578, 669, 811, 917, 1190
AhdI GACNNNNNGTC 1 cut(s) 656
AhlI ACTAGT 1 cut(s) 985
AjiI CACGTC 1 cut(s) 1093
AjnI CCWGG 1 cut(s) 321
AluBI AGCT 6 cut(s) 60, 116, 153, 293, 627, 993
AluI AGCT 6 cut(s) 60, 116, 153, 293, 627, 993
Alw21I GWGCWC 1 cut(s) 838
Alw26I GTCTC 3 cut(s) 502, 541, 946
Alw44I GTGCAC 1 cut(s) 834
AlwI GGATC 1 cut(s) 702
AoxI GGCC 4 cut(s) 398, 1108, 1133, 1232
ApaLI GTGCAC 1 cut(s) 834
ApeKI GCWGC 4 cut(s) 28, 57, 1081, 1104
ApoI RAATTY 1 cut(s) 609
AspLEI GCGC 3 cut(s) 264, 266, 1086
AspS9I GGNCC 1 cut(s) 398
AsuC2I CCSGG 1 cut(s) 1179
BaeGI GKGCMC 1 cut(s) 838
BanII GRGCYC 1 cut(s) 339
Bbv12I GWGCWC 1 cut(s) 838
BbvI GCAGC 4 cut(s) 15, 69, 1068, 1091
BccI CCATC 3 cut(s) 138, 932, 1133
BceAI ACGGC 3 cut(s) 605, 771, 980
BciT130I CCWGG 1 cut(s) 323
BcnI CCSGG 1 cut(s) 1179
BcoDI GTCTC 3 cut(s) 502, 541, 946
BcuI ACTAGT 1 cut(s) 985
BfaI CTAG 2 cut(s) 986, 1056
BglI GCCNNNNNGGC 1 cut(s) 1116
BisI GCNGC 9 cut(s) 29, 58, 1082, 1087, 1105, 1108, 1133, 1254, 1257
BlpI GCTNAGC 1 cut(s) 149
BlsI GCNGC 9 cut(s) 30, 59, 1083, 1088, 1106, 1109, 1134, 1255, 1258
Bme1390I CCNGG 2 cut(s) 323, 1179
BmeRI GACNNNNNGTC 1 cut(s) 656
BmgBI CACGTC 1 cut(s) 1093
BmgT120I GGNCC 1 cut(s) 398
BmrFI CCNGG 2 cut(s) 323, 1179
BmrI ACTGGG 2 cut(s) 516, 893
BmuI ACTGGG 2 cut(s) 516, 893
Bpu1102I GCTNAGC 1 cut(s) 149
BpuEI CTTGAG 1 cut(s) 191
BpuMI CCSGG 1 cut(s) 1179
Bsc4I CCNNNNNNNGG 2 cut(s) 1093, 1241
Bse118I RCCGGY 1 cut(s) 1115
Bse1I ACTGG 6 cut(s) 40, 229, 511, 656, 869, 899
Bse3DI GCAATG 2 cut(s) 252, 884
BseBI CCWGG 1 cut(s) 323
BseGI GGATG 5 cut(s) 149, 652, 660, 1144, 1149
BseLI CCNNNNNNNGG 2 cut(s) 1093, 1241
BseMI GCAATG 2 cut(s) 252, 884
BseNI ACTGG 6 cut(s) 40, 229, 511, 656, 869, 899
BsePI GCGCGC 1 cut(s) 262
BseSI GKGCMC 1 cut(s) 838
BseX3I CGGCCG 1 cut(s) 1133
BseXI GCAGC 4 cut(s) 15, 69, 1068, 1091
Bsh1236I CGCG 2 cut(s) 264, 1089
Bsh1285I CGRYCG 2 cut(s) 1136, 1183
BshFI GGCC 4 cut(s) 400, 1110, 1135, 1234
BsiEI CGRYCG 2 cut(s) 1136, 1183
BsiHKAI GWGCWC 1 cut(s) 838
BsiSI CCGG 4 cut(s) 1078, 1116, 1136, 1179
BslI CCNNNNNNNGG 2 cut(s) 1093, 1241
BsmAI GTCTC 3 cut(s) 502, 541, 946
BsmBI CGTCTC 1 cut(s) 502
BsnI GGCC 4 cut(s) 400, 1110, 1135, 1234
Bsp1286I GDGCHC 2 cut(s) 339, 838
Bsp143I GATC 1 cut(s) 694
Bsp1720I GCTNAGC 1 cut(s) 149
BspACI CCGC 8 cut(s) 26, 1087, 1107, 1132, 1194, 1199, 1253, 1256
BspANI GGCC 4 cut(s) 400, 1110, 1135, 1234
BspFNI CGCG 2 cut(s) 264, 1089
BspPI GGATC 1 cut(s) 702
BsrDI GCAATG 2 cut(s) 252, 884
BsrFI RCCGGY 1 cut(s) 1115
BsrI ACTGG 6 cut(s) 40, 229, 511, 656, 869, 899
BssAI RCCGGY 1 cut(s) 1115
BssHII GCGCGC 1 cut(s) 262
BssMI GATC 1 cut(s) 694
Bst2UI CCWGG 1 cut(s) 323
Bst4CI ACNGT 2 cut(s) 388, 856
BstAPI GCANNNNNTGC 1 cut(s) 890
BstC8I GCNNGC 6 cut(s) 264, 891, 960, 1117, 1121, 1227
BstDEI CTNAG 2 cut(s) 149, 154
BstF5I GGATG 5 cut(s) 149, 652, 660, 1144, 1149
BstFNI CGCG 2 cut(s) 264, 1089
BstHHI GCGC 3 cut(s) 264, 266, 1086
BstKTI GATC 1 cut(s) 697
BstMAI GTCTC 3 cut(s) 502, 541, 946
BstMBI GATC 1 cut(s) 694
BstMCI CGRYCG 2 cut(s) 1136, 1183
BstMWI GCNNNNNNNGC 7 cut(s) 263, 890, 964, 1116, 1129, 1205, 1231
BstNI CCWGG 1 cut(s) 323
BstSCI CCNGG 2 cut(s) 321, 1177
BstSLI GKGCMC 1 cut(s) 838
BstUI CGCG 2 cut(s) 264, 1089
BstV1I GCAGC 4 cut(s) 15, 69, 1068, 1091
BstZI CGGCCG 1 cut(s) 1133
BsuRI GGCC 4 cut(s) 400, 1110, 1135, 1234
BtrI CACGTC 1 cut(s) 1093
BtsCI GGATG 5 cut(s) 149, 652, 660, 1144, 1149
BtsI GCAGTG 2 cut(s) 192, 589
BtsIMutI CAGTG 3 cut(s) 192, 589, 838
Cac8I GCNNGC 6 cut(s) 264, 891, 960, 1117, 1121, 1227
CfoI GCGC 3 cut(s) 264, 266, 1086
Cfr10I RCCGGY 1 cut(s) 1115
Cfr13I GGNCC 1 cut(s) 398
CseI GACGC 1 cut(s) 1112
CsiI ACCWGGT 1 cut(s) 321
Csp6I GTAC 2 cut(s) 319, 1164
CviAII CATG 3 cut(s) 67, 217, 748
CviQI GTAC 2 cut(s) 319, 1164
DdeI CTNAG 2 cut(s) 149, 154
DpnI GATC 1 cut(s) 696
DpnII GATC 1 cut(s) 694
DriI GACNNNNNGTC 1 cut(s) 656
EaeI YGGCCR 1 cut(s) 1133
EagI CGGCCG 1 cut(s) 1133
Eam1105I GACNNNNNGTC 1 cut(s) 656
EclXI CGGCCG 1 cut(s) 1133
Eco147I AGGCCT 1 cut(s) 1234
Eco24I GRGCYC 1 cut(s) 339
Eco52I CGGCCG 1 cut(s) 1133
EcoRII CCWGG 1 cut(s) 321
EcoT22I ATGCAT 2 cut(s) 218, 886
EcoT38I GRGCYC 1 cut(s) 339
Esp3I CGTCTC 1 cut(s) 502
FaeI CATG 3 cut(s) 70, 220, 751
FatI CATG 3 cut(s) 66, 216, 747
Fnu4HI GCNGC 9 cut(s) 29, 58, 1082, 1087, 1105, 1108, 1133, 1254, 1257
FokI GGATG 5 cut(s) 156, 639, 647, 1151, 1156
FriOI GRGCYC 1 cut(s) 339
Fsp4HI GCNGC 9 cut(s) 29, 58, 1082, 1087, 1105, 1108, 1133, 1254, 1257
FspBI CTAG 2 cut(s) 986, 1056
GlaI GCGC 3 cut(s) 263, 265, 1085
GluI GCNGC 9 cut(s) 29, 58, 1082, 1087, 1105, 1108, 1133, 1254, 1257
HaeIII GGCC 4 cut(s) 400, 1110, 1135, 1234
HapII CCGG 4 cut(s) 1078, 1116, 1136, 1179
HgaI GACGC 1 cut(s) 1112
HhaI GCGC 3 cut(s) 264, 266, 1086
Hin1II CATG 3 cut(s) 70, 220, 751
Hin6I GCGC 3 cut(s) 262, 264, 1084
HinP1I GCGC 3 cut(s) 262, 264, 1084
HindIII AAGCTT 1 cut(s) 151
HinfI GANTC 5 cut(s) 14, 22, 221, 794, 811
HpaII CCGG 4 cut(s) 1078, 1116, 1136, 1179
Hpy166II GTNNAC 1 cut(s) 836
Hpy188I TCNGA 6 cut(s) 226, 349, 554, 782, 1185, 1220
Hpy188III TCNNGA 4 cut(s) 208, 304, 377, 951
Hpy8I GTNNAC 1 cut(s) 836
Hpy99I CGWCG 2 cut(s) 1094, 1128
HpyAV CCTTC 4 cut(s) 208, 675, 911, 1184
HpyCH4III ACNGT 2 cut(s) 388, 856
HpyCH4IV ACGT 3 cut(s) 495, 1011, 1092
HpyCH4V TGCA 9 cut(s) 57, 92, 216, 647, 747, 836, 884, 893, 962
HpyF10VI GCNNNNNNNGC 7 cut(s) 263, 890, 964, 1116, 1129, 1205, 1231
HpyF3I CTNAG 2 cut(s) 149, 154
HpySE526I ACGT 3 cut(s) 495, 1011, 1092
Hsp92II CATG 3 cut(s) 70, 220, 751
HspAI GCGC 3 cut(s) 262, 264, 1084
KroI GCCGGC 1 cut(s) 1115
KroNI GCCGGC 1 cut(s) 1117
Kzo9I GATC 1 cut(s) 694
LmnI GCTCC 1 cut(s) 554
Lsp1109I GCAGC 4 cut(s) 15, 69, 1068, 1091
MabI ACCWGGT 1 cut(s) 321
MaeI CTAG 2 cut(s) 986, 1056
MaeII ACGT 3 cut(s) 495, 1011, 1092
MaeIII GTNAC 4 cut(s) 242, 353, 751, 871
MalI GATC 1 cut(s) 696
MboI GATC 1 cut(s) 694
MboII GAAGA 1 cut(s) 55
MhlI GDGCHC 2 cut(s) 339, 838
MluCI AATT 9 cut(s) 39, 171, 297, 408, 435, 609, 819, 840, 946
MlyI GAGTC 2 cut(s) 8, 16
MmeI TCCRAC 4 cut(s) 249, 482, 532, 1163
MnlI CCTC 6 cut(s) 140, 557, 740, 1043, 1121, 1224
Mph1103I ATGCAT 2 cut(s) 218, 886
MreI CGCCGGCG 1 cut(s) 1115
MroNI GCCGGC 1 cut(s) 1115
MseI TTAA 5 cut(s) 78, 434, 596, 773, 1044
MslI CAYNNNNRTG 2 cut(s) 314, 746
MspA1I CMGCKG 1 cut(s) 28
MspI CCGG 4 cut(s) 1078, 1116, 1136, 1179
MspR9I CCNGG 2 cut(s) 323, 1179
MvaI CCWGG 1 cut(s) 323
MvnI CGCG 2 cut(s) 264, 1089
MwoI GCNNNNNNNGC 7 cut(s) 263, 890, 964, 1116, 1129, 1205, 1231
NaeI GCCGGC 1 cut(s) 1117
NciI CCSGG 1 cut(s) 1179
NdeII GATC 1 cut(s) 694
NgoMIV GCCGGC 1 cut(s) 1115
NlaIII CATG 3 cut(s) 70, 220, 751
NmeAIII GCCGAG 2 cut(s) 283, 479
NmuCI GTSAC 1 cut(s) 353
NsiI ATGCAT 2 cut(s) 218, 886
PauI GCGCGC 1 cut(s) 262
PceI AGGCCT 1 cut(s) 1234
PdiI GCCGGC 1 cut(s) 1117
PfeI GAWTC 3 cut(s) 221, 794, 811
PkrI GCNGC 9 cut(s) 30, 59, 1083, 1088, 1106, 1109, 1134, 1255, 1258
PleI GAGTC 2 cut(s) 8, 16
PpsI GAGTC 2 cut(s) 8, 16
PsiI TTATAA 1 cut(s) 971
Psp6I CCWGG 1 cut(s) 321
PspGI CCWGG 1 cut(s) 321
PspPI GGNCC 1 cut(s) 398
PteI GCGCGC 1 cut(s) 262
RsaI GTAC 2 cut(s) 320, 1165
RsaNI GTAC 2 cut(s) 319, 1164
RseI CAYNNNNRTG 2 cut(s) 314, 746
SaqAI TTAA 5 cut(s) 78, 434, 596, 773, 1044
SatI GCNGC 9 cut(s) 29, 58, 1082, 1087, 1105, 1108, 1133, 1254, 1257
Sau3AI GATC 1 cut(s) 694
Sau96I GGNCC 1 cut(s) 398
SchI GAGTC 2 cut(s) 8, 16
ScrFI CCNGG 2 cut(s) 323, 1179
SduI GDGCHC 2 cut(s) 339, 838
SexAI ACCWGGT 1 cut(s) 321
SgrAI CRCCGGYG 1 cut(s) 1115
SmiMI CAYNNNNRTG 2 cut(s) 314, 746
SmlI CTYRAG 2 cut(s) 16, 206
SmoI CTYRAG 2 cut(s) 16, 206
SpeI ACTAGT 1 cut(s) 985
Sse9I AATT 9 cut(s) 39, 171, 297, 408, 435, 609, 819, 840, 946
SseBI AGGCCT 1 cut(s) 1234
SsiI CCGC 8 cut(s) 26, 1087, 1107, 1132, 1194, 1199, 1253, 1256
SspMI CTAG 2 cut(s) 986, 1056
StuI AGGCCT 1 cut(s) 1234
StyD4I CCNGG 2 cut(s) 321, 1177
TaaI ACNGT 2 cut(s) 388, 856
TaiI ACGT 3 cut(s) 498, 1014, 1095
TaqI TCGA 3 cut(s) 12, 950, 1126
TasI AATT 9 cut(s) 39, 171, 297, 408, 435, 609, 819, 840, 946
TauI GCSGC 5 cut(s) 1089, 1110, 1135, 1256, 1259
TfiI GAWTC 3 cut(s) 221, 794, 811
Tru1I TTAA 5 cut(s) 78, 434, 596, 773, 1044
Tru9I TTAA 5 cut(s) 78, 434, 596, 773, 1044
TscAI CASTG 3 cut(s) 199, 589, 838
TseFI GTSAC 1 cut(s) 353
TseI GCWGC 4 cut(s) 28, 57, 1081, 1104
Tsp45I GTSAC 1 cut(s) 353
TspDTI ATGAA 2 cut(s) 55, 1187
TspRI CASTG 3 cut(s) 199, 589, 838
VneI GTGCAC 1 cut(s) 834
XapI RAATTY 1 cut(s) 609
XspI CTAG 2 cut(s) 986, 1056
Zsp2I ATGCAT 2 cut(s) 218, 886
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.