Rw5G035450

Vacuolar amino acid transporter 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0006420
Physical Location & Seq
Reverse (-)
61378010 .. 61380097
2088 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G035450.1

Sequence Viewer

Length: 1212 bp
ATGAACAGTGAAAGTACTACTCCTTTGACGGGCCAATCTAGCCTTTGGAAAGCTACGTTTAATACAACTAGTGTGTTTTTAGGGATAGGCATTATTACTCTACCGTACGCACTCTCCAGAGGAGGGTGGGTATCGCTCCTTCTTTGCATTGTCATCGCCATGATGACATGCTATACTGGCATGCTACTAAAAGCATGCATGGATGCAAACCCATCATTAACCACGTACCTCGATATTGTCGAACGAGCATTTGGCAATAAATGGAGGATTATAGCTTCCGTGTTTTTAAACACGGAACTATACTTAGTCGCTCTCAGCTTGATGATAGTCGAAAGTGAAAATTTACACAAATTATTCCCCACGTTTGCCATTAAGCATGGTGGCGGGGCAACAATAATTAGCGGTAGAAAGACTTTCGTCTTAATTACCGCTTGCATTACTTTACCGACAATGCTGCTCACGGATTTGAGCATATTGTCGTACGTGTCGGTCATGGGAGTTCTTTCTTCTCTCCTTATCGTGGGATCGCTCATATCCGTCGGAATATCAAACGTTGGCTTTCACACACAAGGGGGTCAGCTGTGGAACATGAGTGGAATCCCAACCGCTATGAGTTTCTATATTTTTTGCTTCGCGGGCCACCCGGTTCTACCTTCCATTTATGTGTCAATGAGACATCGACAACAATTCATTAAGGTGTTGATGATTAGCTTTCTTCTCACCACCACGACTTGCTTGGTTATAGCTGTGATTGGCTATCTTATGTATGGAGGAGGAGTGCAATCTCAGATAACCCTCAACCTCCCCACCTCAGAAGCAGGTGCGAAAGTCGCTATTTATACATTACTATTAATACCGATTACCCGCTTTGCATTGTTGGTTACCCCTGTAGCTTCGTCTATTGAGGGTCACCTCCCTCAGAAGTACCAAAATAGGAGTTTAATTCAGAGAGCTGTTAGGCTAGTAATTTTGGCTAGCAGTACCTTATTAGCTTATTTCTTTCCCTATTTTGAGACCCTAGTGGGCATACTAGGATCCATTTTTATTGTATTAGCATCTTTTTTATTGCCTTGTGCATGCTACATCAAGCTTACTCACAGCTTTAGTGCATGGAGCTACAAGTTAGTAGCCACTTTAGCTATAATGGTGTTTGGTGTTCTTGCCGGAGTTCTAGGCACATATACAGCCATTGTTGATCTTGTAAACCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04721 ko04723 ko04727 ko05032 ko05033 map04721 map04723 map04727 map05032 map05033
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

403

Amino Acids

43.77

Weight (kDa)

9.15

Isoelectric Point (pI)

33.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 12 - 397 5.5e-60 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 809
Acc36I ACCTGC 1 cut(s) 809
AccII CGCG 1 cut(s) 635
AciI CCGC 6 cut(s) 384, 402, 429, 606, 635, 865
AclI AACGTT 1 cut(s) 552
AclWI GGATC 3 cut(s) 532, 1029, 1042
AcsI RAATTY 1 cut(s) 340
AfaI GTAC 6 cut(s) 16, 107, 227, 482, 926, 982
AfiI CCNNNNNNNGG 3 cut(s) 29, 123, 520
AflIII ACRYGT 1 cut(s) 483
AhlI ACTAGT 1 cut(s) 68
AjuI GAANNNNNNNTTGG 2 cut(s) 234, 266
Alw26I GTCTC 2 cut(s) 667, 1007
AlwI GGATC 3 cut(s) 532, 1029, 1042
AoxI GGCC 2 cut(s) 31, 637
ApeKI GCWGC 1 cut(s) 454
ApoI RAATTY 1 cut(s) 340
AseI ATTAAT 1 cut(s) 851
AspS9I GGNCC 2 cut(s) 31, 637
AsuC2I CCSGG 1 cut(s) 644
AsuHPI GGTGA 2 cut(s) 712, 902
AsuNHI GCTAGC 1 cut(s) 974
BamHI GGATCC 1 cut(s) 1034
BarI GAAGNNNNNNTAC 2 cut(s) 123, 155
BbvI GCAGC 1 cut(s) 441
BccI CCATC 1 cut(s) 220
BcgI CGANNNNNNTGC 4 cut(s) 136, 170, 436, 470
BcnI CCSGG 1 cut(s) 644
BcoDI GTCTC 2 cut(s) 667, 1007
BcuI ACTAGT 1 cut(s) 68
BfaI CTAG 7 cut(s) 39, 69, 962, 975, 1019, 1031, 1172
BfmI CTRYAG 1 cut(s) 888
BfuAI ACCTGC 1 cut(s) 809
BisI GCNGC 1 cut(s) 455
BlsI GCNGC 1 cut(s) 456
BmcAI AGTACT 1 cut(s) 16
Bme1390I CCNGG 1 cut(s) 644
BmgT120I GGNCC 2 cut(s) 31, 637
BmiI GGNNCC 1 cut(s) 1036
BmrFI CCNGG 1 cut(s) 644
BmsI GCATC 2 cut(s) 193, 1064
BmtI GCTAGC 1 cut(s) 978
BoxI GACNNNNGTC 1 cut(s) 416
BpmI CTGGAG 1 cut(s) 100
BpuMI CCSGG 1 cut(s) 644
BsaAI YACGTR 2 cut(s) 225, 484
BsaI GGTCTC 1 cut(s) 1007
BsaXI ACNNNNNCTCC 6 cut(s) 98, 114, 128, 144, 762, 792
Bsc4I CCNNNNNNNGG 3 cut(s) 29, 123, 520
Bse1I ACTGG 1 cut(s) 181
BseGI GGATG 1 cut(s) 208
BseLI CCNNNNNNNGG 3 cut(s) 29, 123, 520
BseMII CTCAG 4 cut(s) 328, 800, 825, 932
BseNI ACTGG 1 cut(s) 181
BseRI GAGGAG 3 cut(s) 135, 786, 789
BseXI GCAGC 1 cut(s) 441
Bsh1236I CGCG 1 cut(s) 635
BshFI GGCC 2 cut(s) 33, 639
BsiSI CCGG 2 cut(s) 644, 1164
BsiWI CGTACG 2 cut(s) 105, 480
BslI CCNNNNNNNGG 3 cut(s) 29, 123, 520
BsmAI GTCTC 2 cut(s) 667, 1007
BsnI GGCC 2 cut(s) 33, 639
Bso31I GGTCTC 1 cut(s) 1007
Bsp143I GATC 3 cut(s) 524, 1034, 1195
BspACI CCGC 6 cut(s) 384, 402, 429, 606, 635, 865
BspANI GGCC 2 cut(s) 33, 639
BspCNI CTCAG 4 cut(s) 327, 799, 824, 931
BspFNI CGCG 1 cut(s) 635
BspLI GGNNCC 1 cut(s) 1036
BspMI ACCTGC 1 cut(s) 809
BspOI GCTAGC 1 cut(s) 978
BspPI GGATC 3 cut(s) 532, 1029, 1042
BspTNI GGTCTC 1 cut(s) 1007
BsrI ACTGG 1 cut(s) 181
BssMI GATC 3 cut(s) 524, 1034, 1195
Bst4CI ACNGT 2 cut(s) 8, 105
BstBAI YACGTR 2 cut(s) 225, 484
BstC8I GCNNGC 6 cut(s) 182, 196, 433, 637, 976, 1078
BstDEI CTNAG 5 cut(s) 304, 314, 786, 811, 918
BstEII GGTNACC 2 cut(s) 880, 908
BstF5I GGATG 1 cut(s) 208
BstFNI CGCG 1 cut(s) 635
BstKTI GATC 3 cut(s) 527, 1037, 1198
BstMAI GTCTC 2 cut(s) 667, 1007
BstMBI GATC 3 cut(s) 524, 1034, 1195
BstMWI GCNNNNNNNGC 5 cut(s) 39, 177, 636, 830, 1136
BstNSI RCATGY 4 cut(s) 171, 184, 198, 1080
BstPAI GACNNNNGTC 1 cut(s) 416
BstPI GGTNACC 2 cut(s) 880, 908
BstSCI CCNGG 1 cut(s) 642
BstSFI CTRYAG 1 cut(s) 888
BstUI CGCG 1 cut(s) 635
BstV1I GCAGC 1 cut(s) 441
BstX2I RGATCY 1 cut(s) 1034
BstYI RGATCY 1 cut(s) 1034
BsuRI GGCC 2 cut(s) 33, 639
BtgZI GCGATG 1 cut(s) 139
BtsCI GGATG 1 cut(s) 208
BtsIMutI CAGTG 1 cut(s) 13
BveI ACCTGC 1 cut(s) 809
Cac8I GCNNGC 6 cut(s) 182, 196, 433, 637, 976, 1078
Cfr13I GGNCC 2 cut(s) 31, 637
Csp6I GTAC 6 cut(s) 15, 106, 226, 481, 925, 981
CviQI GTAC 6 cut(s) 15, 106, 226, 481, 925, 981
DdeI CTNAG 5 cut(s) 304, 314, 786, 811, 918
DpnI GATC 3 cut(s) 526, 1036, 1197
DpnII GATC 3 cut(s) 524, 1034, 1195
DraI TTTAAA 1 cut(s) 288
Eco31I GGTCTC 1 cut(s) 1007
Eco91I GGTNACC 2 cut(s) 880, 908
EcoO65I GGTNACC 2 cut(s) 880, 908
EcoT22I ATGCAT 1 cut(s) 200
FauI CCCGC 3 cut(s) 377, 628, 872
Fnu4HI GCNGC 1 cut(s) 455
FokI GGATG 1 cut(s) 215
Fsp4HI GCNGC 1 cut(s) 455
FspBI CTAG 7 cut(s) 39, 69, 962, 975, 1019, 1031, 1172
GluI GCNGC 1 cut(s) 455
GsuI CTGGAG 1 cut(s) 100
HaeIII GGCC 2 cut(s) 33, 639
HapII CCGG 2 cut(s) 644, 1164
HindIII AAGCTT 1 cut(s) 1088
HinfI GANTC 1 cut(s) 597
HpaII CCGG 2 cut(s) 644, 1164
HphI GGTGA 2 cut(s) 712, 902
Hpy166II GTNNAC 1 cut(s) 1204
Hpy188I TCNGA 5 cut(s) 542, 789, 814, 921, 948
Hpy188III TCNNGA 1 cut(s) 117
Hpy8I GTNNAC 1 cut(s) 1204
Hpy99I CGWCG 1 cut(s) 542
HpyAV CCTTC 2 cut(s) 149, 663
HpyCH4III ACNGT 2 cut(s) 8, 105
HpyCH4IV ACGT 5 cut(s) 56, 224, 362, 483, 552
HpyCH4V TGCA 8 cut(s) 147, 198, 206, 435, 781, 872, 1076, 1109
HpyF10VI GCNNNNNNNGC 5 cut(s) 39, 177, 636, 830, 1136
HpyF3I CTNAG 5 cut(s) 304, 314, 786, 811, 918
HpySE526I ACGT 5 cut(s) 56, 224, 362, 483, 552
Kzo9I GATC 3 cut(s) 524, 1034, 1195
LmnI GCTCC 2 cut(s) 141, 1113
LpnPI CCDG 6 cut(s) 130, 162, 657, 804, 900, 1177
Lsp1109I GCAGC 1 cut(s) 441
LweI GCATC 2 cut(s) 193, 1064
MaeI CTAG 7 cut(s) 39, 69, 962, 975, 1019, 1031, 1172
MaeII ACGT 5 cut(s) 56, 224, 362, 483, 552
MaeIII GTNAC 2 cut(s) 880, 908
MalI GATC 3 cut(s) 526, 1036, 1197
MboI GATC 3 cut(s) 524, 1034, 1195
MboII GAAGA 2 cut(s) 498, 707
MflI RGATCY 1 cut(s) 1034
MluCI AATT 7 cut(s) 340, 350, 396, 423, 686, 942, 966
MmeI TCCRAC 1 cut(s) 520
Mph1103I ATGCAT 1 cut(s) 200
MseI TTAA 9 cut(s) 60, 218, 287, 372, 422, 693, 851, 941, 1210
MslI CAYNNNNRTG 3 cut(s) 158, 662, 695
MspA1I CMGCKG 1 cut(s) 580
MspI CCGG 2 cut(s) 644, 1164
MspR9I CCNGG 1 cut(s) 644
MvnI CGCG 1 cut(s) 635
MwoI GCNNNNNNNGC 5 cut(s) 39, 177, 636, 830, 1136
NciI CCSGG 1 cut(s) 644
NdeII GATC 3 cut(s) 524, 1034, 1195
NheI GCTAGC 1 cut(s) 974
NlaIV GGNNCC 1 cut(s) 1036
NmuCI GTSAC 1 cut(s) 908
NsiI ATGCAT 1 cut(s) 200
NspI RCATGY 4 cut(s) 171, 184, 198, 1080
PaeI GCATGC 3 cut(s) 184, 198, 1080
PaqCI CACCTGC 1 cut(s) 809
PcsI WCGNNNNNNNCGW 1 cut(s) 237
PfeI GAWTC 1 cut(s) 597
Pfl23II CGTACG 2 cut(s) 105, 480
PkrI GCNGC 1 cut(s) 456
Ppu21I YACGTR 2 cut(s) 225, 484
PshAI GACNNNNGTC 1 cut(s) 416
PshBI ATTAAT 1 cut(s) 851
Psp1406I AACGTT 1 cut(s) 552
PspEI GGTNACC 2 cut(s) 880, 908
PspLI CGTACG 2 cut(s) 105, 480
PspN4I GGNNCC 1 cut(s) 1036
PspPI GGNCC 2 cut(s) 31, 637
PsuI RGATCY 1 cut(s) 1034
PvuII CAGCTG 1 cut(s) 580
RsaI GTAC 6 cut(s) 16, 107, 227, 482, 926, 982
RsaNI GTAC 6 cut(s) 15, 106, 226, 481, 925, 981
RseI CAYNNNNRTG 3 cut(s) 158, 662, 695
SaqAI TTAA 9 cut(s) 60, 218, 287, 372, 422, 693, 851, 941, 1210
SatI GCNGC 1 cut(s) 455
Sau3AI GATC 3 cut(s) 524, 1034, 1195
Sau96I GGNCC 2 cut(s) 31, 637
ScaI AGTACT 1 cut(s) 16
ScrFI CCNGG 1 cut(s) 644
SfaNI GCATC 2 cut(s) 193, 1064
SfcI CTRYAG 1 cut(s) 888
SmiMI CAYNNNNRTG 3 cut(s) 158, 662, 695
SpeI ACTAGT 1 cut(s) 68
SphI GCATGC 3 cut(s) 184, 198, 1080
Sse9I AATT 7 cut(s) 340, 350, 396, 423, 686, 942, 966
SsiI CCGC 6 cut(s) 384, 402, 429, 606, 635, 865
SspMI CTAG 7 cut(s) 39, 69, 962, 975, 1019, 1031, 1172
StyD4I CCNGG 1 cut(s) 642
TaaI ACNGT 2 cut(s) 8, 105
TaiI ACGT 5 cut(s) 59, 227, 365, 486, 555
TaqI TCGA 4 cut(s) 231, 240, 330, 679
TaqII GACCGA 1 cut(s) 478
TasI AATT 7 cut(s) 340, 350, 396, 423, 686, 942, 966
TatI WGTACW 1 cut(s) 14
TfiI GAWTC 1 cut(s) 597
Tru1I TTAA 9 cut(s) 60, 218, 287, 372, 422, 693, 851, 941, 1210
Tru9I TTAA 9 cut(s) 60, 218, 287, 372, 422, 693, 851, 941, 1210
TscAI CASTG 1 cut(s) 13
TseFI GTSAC 1 cut(s) 908
TseI GCWGC 1 cut(s) 454
Tsp45I GTSAC 1 cut(s) 908
TspDTI ATGAA 2 cut(s) 17, 679
TspGWI ACGGA 4 cut(s) 268, 308, 476, 526
TspRI CASTG 1 cut(s) 13
VspI ATTAAT 1 cut(s) 851
XapI RAATTY 1 cut(s) 340
XceI RCATGY 4 cut(s) 171, 184, 198, 1080
XcmI CCANNNNNNNNNTGG 1 cut(s) 733
XspI CTAG 7 cut(s) 39, 69, 962, 975, 1019, 1031, 1172
ZrmI AGTACT 1 cut(s) 16
Zsp2I ATGCAT 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.