Rh5DG180600

Vacuolar amino acid transporter 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0006420
Physical Location & Seq
Reverse (-)
19967808 .. 19972952
5145 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG180600.1

Sequence Viewer

Length: 900 bp
ATGGATTCCGACCCGTCAATCACAAGTTACCTTGACATTGCCGAGCGCGCATTTGATAAAAGAGCCAGAACCATAGCTTCAATTTTCTTGAACACAGAAATGTACCTGGTCGCCACAGGGCTCATAATATCAGAAAGTGACAACTTACATAAGTTGTTTTCAGACTTCAAGATGGATTTCGGGCCATTGGTAATCGGCGGTAGGCAGTCGTTTGTGTTAATTACAGCATTGATTATATCTCCAACGATGATGATGATTGATTTGAGTGTATTGTCATACGTCTCGGCTACTGGGGTGTTTTCCACCCTTGTCATTGTTGTCTCCCTTGTGTGTGTTGGAGCATTTGGAGGTGTTGGGTTTCAAGGCAGTGGAGTTTTGTTAAACGTGAGTGGGATTCCTACTGCTGTGAGCTTGTATATCTTTTGCTTTGCAGGACATCCAGTCATCCCTTCAATATACATTTCTATGCGAAAGAGGGATCAGTTCGGTAAGGTCTTGTTCGTTATCTACTTCCTCACAACTATCACTTGCATGGTAACGGCTGTTACAGGCTATTTAATGTTCGGAAATAACACCGAATCTCAAATCACTTTGAATCTACCAATTACAGAATTCAGTGCACAAATTACAATCTCCACCGTTCTACTTATTCCAGTAACAAGATATGCATTGCATGCAACCCCAGTTGCTAATGTCATTGAAGGTGGAGTATTATCAAAAGATGGCAACAATTCGAGACTGCTTGCACGGCTTATAAGGTTGGGATTACTAGCTAAGAGGATTCAAGTGACCGAAGTACGGATGAGGAAGAGTATTCCAGCACAAGAGTTGATGAGTCTGCGTAGCAAGCCGAGCATGATTTTCAAAAATTATTTGTCATCCCCTGTCGATTTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04721 ko04723 ko04727 ko05032 ko05033 map04721 map04723 map04727 map05032 map05033
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

299

Amino Acids

32.66

Weight (kDa)

9.13

Isoelectric Point (pI)

32.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 3 - 257 5.9e-34 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 755
AccII CGCG 1 cut(s) 48
AciI CCGC 1 cut(s) 198
AclWI GGATC 1 cut(s) 486
AcsI RAATTY 1 cut(s) 611
AfaI GTAC 2 cut(s) 104, 798
AfiI CCNNNNNNNGG 1 cut(s) 798
AgsI TTSAA 9 cut(s) 81, 91, 169, 362, 453, 595, 701, 785, 865
AhdI GACNNNNNGTC 1 cut(s) 440
AjnI CCWGG 1 cut(s) 105
AluBI AGCT 3 cut(s) 77, 411, 773
AluI AGCT 3 cut(s) 77, 411, 773
Alw21I GWGCWC 1 cut(s) 622
Alw26I GTCTC 3 cut(s) 286, 325, 730
Alw44I GTGCAC 1 cut(s) 618
AlwI GGATC 1 cut(s) 486
AoxI GGCC 1 cut(s) 182
ApaLI GTGCAC 1 cut(s) 618
ApoI RAATTY 1 cut(s) 611
AspLEI GCGC 2 cut(s) 48, 50
AspS9I GGNCC 1 cut(s) 182
BaeGI GKGCMC 1 cut(s) 622
BanII GRGCYC 1 cut(s) 123
Bbv12I GWGCWC 1 cut(s) 622
BccI CCATC 2 cut(s) 166, 716
BceAI ACGGC 2 cut(s) 555, 764
BciT130I CCWGG 1 cut(s) 107
BcoDI GTCTC 3 cut(s) 286, 325, 730
BfaI CTAG 1 cut(s) 770
Bme1390I CCNGG 1 cut(s) 107
BmeRI GACNNNNNGTC 1 cut(s) 440
BmgT120I GGNCC 1 cut(s) 182
BmrFI CCNGG 1 cut(s) 107
BmrI ACTGGG 2 cut(s) 300, 677
BmuI ACTGGG 2 cut(s) 300, 677
Bsc4I CCNNNNNNNGG 1 cut(s) 798
Bse1I ACTGG 4 cut(s) 295, 440, 653, 683
Bse3DI GCAATG 2 cut(s) 36, 668
BseBI CCWGG 1 cut(s) 107
BseGI GGATG 4 cut(s) 436, 444, 807, 878
BseLI CCNNNNNNNGG 1 cut(s) 798
BseMI GCAATG 2 cut(s) 36, 668
BseNI ACTGG 4 cut(s) 295, 440, 653, 683
BsePI GCGCGC 1 cut(s) 46
BseSI GKGCMC 1 cut(s) 622
Bsh1236I CGCG 1 cut(s) 48
BshFI GGCC 1 cut(s) 184
BsiHKAI GWGCWC 1 cut(s) 622
BslI CCNNNNNNNGG 1 cut(s) 798
BsmAI GTCTC 3 cut(s) 286, 325, 730
BsmBI CGTCTC 1 cut(s) 286
BsnI GGCC 1 cut(s) 184
Bsp1286I GDGCHC 2 cut(s) 123, 622
Bsp143I GATC 1 cut(s) 478
BspACI CCGC 1 cut(s) 198
BspANI GGCC 1 cut(s) 184
BspFNI CGCG 1 cut(s) 48
BspPI GGATC 1 cut(s) 486
BsrDI GCAATG 2 cut(s) 36, 668
BsrI ACTGG 4 cut(s) 295, 440, 653, 683
BssHII GCGCGC 1 cut(s) 46
BssMI GATC 1 cut(s) 478
Bst2UI CCWGG 1 cut(s) 107
Bst4CI ACNGT 1 cut(s) 640
Bst6I CTCTTC 1 cut(s) 803
BstAPI GCANNNNNTGC 1 cut(s) 674
BstC8I GCNNGC 4 cut(s) 48, 675, 744, 848
BstDEI CTNAG 1 cut(s) 774
BstF5I GGATG 4 cut(s) 436, 444, 807, 878
BstFNI CGCG 1 cut(s) 48
BstHHI GCGC 2 cut(s) 48, 50
BstKTI GATC 1 cut(s) 481
BstMAI GTCTC 3 cut(s) 286, 325, 730
BstMBI GATC 1 cut(s) 478
BstMWI GCNNNNNNNGC 5 cut(s) 47, 674, 748, 847, 852
BstNI CCWGG 1 cut(s) 107
BstNSI RCATGY 1 cut(s) 677
BstSCI CCNGG 1 cut(s) 105
BstSLI GKGCMC 1 cut(s) 622
BstUI CGCG 1 cut(s) 48
BsuRI GGCC 1 cut(s) 184
BtsCI GGATG 4 cut(s) 436, 444, 807, 878
BtsI GCAGTG 1 cut(s) 373
BtsIMutI CAGTG 2 cut(s) 373, 622
Cac8I GCNNGC 4 cut(s) 48, 675, 744, 848
CfoI GCGC 2 cut(s) 48, 50
Cfr13I GGNCC 1 cut(s) 182
CsiI ACCWGGT 1 cut(s) 105
Csp6I GTAC 2 cut(s) 103, 797
CviAII CATG 3 cut(s) 532, 674, 856
CviQI GTAC 2 cut(s) 103, 797
DdeI CTNAG 1 cut(s) 774
DpnI GATC 1 cut(s) 480
DpnII GATC 1 cut(s) 478
DriI GACNNNNNGTC 1 cut(s) 440
Eam1104I CTCTTC 1 cut(s) 803
Eam1105I GACNNNNNGTC 1 cut(s) 440
EarI CTCTTC 1 cut(s) 803
Eco24I GRGCYC 1 cut(s) 123
EcoRI GAATTC 1 cut(s) 611
EcoRII CCWGG 1 cut(s) 105
EcoT22I ATGCAT 1 cut(s) 670
EcoT38I GRGCYC 1 cut(s) 123
Esp3I CGTCTC 1 cut(s) 286
FaeI CATG 3 cut(s) 535, 677, 859
FatI CATG 3 cut(s) 531, 673, 855
FokI GGATG 4 cut(s) 423, 431, 814, 865
FriOI GRGCYC 1 cut(s) 123
FspBI CTAG 1 cut(s) 770
GlaI GCGC 2 cut(s) 47, 49
HaeIII GGCC 1 cut(s) 184
HhaI GCGC 2 cut(s) 48, 50
Hin1II CATG 3 cut(s) 535, 677, 859
Hin6I GCGC 2 cut(s) 46, 48
HinP1I GCGC 2 cut(s) 46, 48
HinfI GANTC 6 cut(s) 5, 394, 578, 595, 781, 835
Hpy166II GTNNAC 1 cut(s) 620
Hpy188I TCNGA 4 cut(s) 10, 133, 163, 566
Hpy188III TCNNGA 3 cut(s) 88, 169, 735
Hpy8I GTNNAC 1 cut(s) 620
HpyAV CCTTC 2 cut(s) 459, 695
HpyCH4III ACNGT 1 cut(s) 640
HpyCH4IV ACGT 2 cut(s) 279, 384
HpyCH4V TGCA 7 cut(s) 431, 531, 620, 668, 673, 677, 746
HpyF10VI GCNNNNNNNGC 5 cut(s) 47, 674, 748, 847, 852
HpyF3I CTNAG 1 cut(s) 774
HpySE526I ACGT 2 cut(s) 279, 384
Hsp92II CATG 3 cut(s) 535, 677, 859
HspAI GCGC 2 cut(s) 46, 48
Kzo9I GATC 1 cut(s) 478
LmnI GCTCC 1 cut(s) 338
MabI ACCWGGT 1 cut(s) 105
MaeI CTAG 1 cut(s) 770
MaeII ACGT 2 cut(s) 279, 384
MaeIII GTNAC 6 cut(s) 26, 137, 535, 544, 655, 787
MalI GATC 1 cut(s) 480
MboI GATC 1 cut(s) 478
MboII GAAGA 1 cut(s) 820
MhlI GDGCHC 2 cut(s) 123, 622
MluCI AATT 7 cut(s) 81, 219, 603, 611, 624, 730, 868
MlyI GAGTC 1 cut(s) 844
MmeI TCCRAC 3 cut(s) 33, 266, 316
MnlI CCTC 5 cut(s) 341, 468, 524, 771, 798
Mph1103I ATGCAT 1 cut(s) 670
MseI TTAA 3 cut(s) 218, 380, 557
MslI CAYNNNNRTG 3 cut(s) 98, 464, 530
MspR9I CCNGG 1 cut(s) 107
MvaI CCWGG 1 cut(s) 107
MvnI CGCG 1 cut(s) 48
MwoI GCNNNNNNNGC 5 cut(s) 47, 674, 748, 847, 852
NdeII GATC 1 cut(s) 478
NlaIII CATG 3 cut(s) 535, 677, 859
NmeAIII GCCGAG 3 cut(s) 67, 263, 876
NmuCI GTSAC 2 cut(s) 137, 787
NsiI ATGCAT 1 cut(s) 670
NspI RCATGY 1 cut(s) 677
PaeI GCATGC 1 cut(s) 677
PauI GCGCGC 1 cut(s) 46
PfeI GAWTC 5 cut(s) 5, 394, 578, 595, 781
PleI GAGTC 1 cut(s) 843
PpsI GAGTC 1 cut(s) 843
PsiI TTATAA 1 cut(s) 755
Psp6I CCWGG 1 cut(s) 105
PspGI CCWGG 1 cut(s) 105
PspPI GGNCC 1 cut(s) 182
PteI GCGCGC 1 cut(s) 46
RsaI GTAC 2 cut(s) 104, 798
RsaNI GTAC 2 cut(s) 103, 797
RseI CAYNNNNRTG 3 cut(s) 98, 464, 530
SaqAI TTAA 3 cut(s) 218, 380, 557
Sau3AI GATC 1 cut(s) 478
Sau96I GGNCC 1 cut(s) 182
SchI GAGTC 1 cut(s) 844
ScrFI CCNGG 1 cut(s) 107
SduI GDGCHC 2 cut(s) 123, 622
SexAI ACCWGGT 1 cut(s) 105
SmiMI CAYNNNNRTG 3 cut(s) 98, 464, 530
SphI GCATGC 1 cut(s) 677
Sse9I AATT 7 cut(s) 81, 219, 603, 611, 624, 730, 868
SsiI CCGC 1 cut(s) 198
SspMI CTAG 1 cut(s) 770
StyD4I CCNGG 1 cut(s) 105
TaaI ACNGT 1 cut(s) 640
TaiI ACGT 2 cut(s) 282, 387
TaqI TCGA 2 cut(s) 734, 888
TaqII GACCGA 1 cut(s) 806
TasI AATT 7 cut(s) 81, 219, 603, 611, 624, 730, 868
TfiI GAWTC 5 cut(s) 5, 394, 578, 595, 781
Tru1I TTAA 3 cut(s) 218, 380, 557
Tru9I TTAA 3 cut(s) 218, 380, 557
TscAI CASTG 2 cut(s) 373, 622
TseFI GTSAC 2 cut(s) 137, 787
Tsp45I GTSAC 2 cut(s) 137, 787
TspGWI ACGGA 1 cut(s) 814
TspRI CASTG 2 cut(s) 373, 622
VneI GTGCAC 1 cut(s) 618
XapI RAATTY 1 cut(s) 611
XceI RCATGY 1 cut(s) 677
XspI CTAG 1 cut(s) 770
Zsp2I ATGCAT 1 cut(s) 670
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.