RchiOBHm_Chr6g0284151

Membrane protein of ER body-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0002486
Physical Location & Seq
Reverse (-)
47432895 .. 47433736
842 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ25483

Sequence Viewer

Length: 447 bp
ATGTCACTCTGGGACCTGAAAGCTAATCAAGTAGACAGCAAATACAAAGAAGTACTAGGGAACAAAGATAATTTCTTTTTTCATGCCCCGGTTGCCATACTCTCGTTCCTCATTTTCGGGTTAGTACCTCCTTTGGTTTATGGCTTCTCGTTCTCTCATAGCGACGACAGCGACCTAAAGATTGCAGCAGTTGCATCATCTTCTATTTTATGCATCATACTTCTCGCAATTGGAAAGGCTTACATCCAGACGCCACCAAAGTACATCAAAAATGTGTTGTACTATATCGTCATAGGGGTTGGCGCTGCCGGTGTTTCTTACTTGGTAGGTGACTTAATTGACAAACTTGTAGAGAAGCTTGGCTGGTTCAACTCAACTGTAGATGGCTCTCTGCCTCTTCCTCATCTGACTATGAGTACAGTGAAACCTGCATGGGAATCCTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VIT1

Protein Analysis

148

Amino Acids

16.25

Weight (kDa)

6.25

Isoelectric Point (pI)

28.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VIT1 PF01988 28 - 110 6.5e-09 VIT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 436
AccI GTMKAC 1 cut(s) 33
AcyI GRCGYC 1 cut(s) 251
AfaI GTAC 5 cut(s) 54, 126, 263, 281, 418
AgsI TTSAA 1 cut(s) 370
AjnI CCWGG 1 cut(s) 440
AluBI AGCT 2 cut(s) 23, 358
AluI AGCT 2 cut(s) 23, 358
ApeKI GCWGC 2 cut(s) 185, 305
AspLEI GCGC 1 cut(s) 305
AspS9I GGNCC 1 cut(s) 13
AsuC2I CCSGG 1 cut(s) 89
AsuHPI GGTGA 1 cut(s) 341
AvaII GGWCC 1 cut(s) 13
BbvI GCAGC 2 cut(s) 197, 292
BccI CCATC 1 cut(s) 377
BciT130I CCWGG 1 cut(s) 442
BcnI CCSGG 1 cut(s) 89
BfaI CTAG 1 cut(s) 56
BfmI CTRYAG 1 cut(s) 378
BfoI RGCGCY 1 cut(s) 306
BfuAI ACCTGC 1 cut(s) 436
BisI GCNGC 2 cut(s) 186, 306
BlsI GCNGC 2 cut(s) 187, 307
BmcAI AGTACT 1 cut(s) 54
Bme1390I CCNGG 2 cut(s) 89, 442
Bme18I GGWCC 1 cut(s) 13
BmgT120I GGNCC 1 cut(s) 13
BmiI GGNNCC 1 cut(s) 14
BmrFI CCNGG 2 cut(s) 89, 442
BmsI GCATC 2 cut(s) 203, 222
BpuMI CCSGG 1 cut(s) 89
BsaHI GRCGYC 1 cut(s) 251
BsaJI CCNNGG 1 cut(s) 87
Bse118I RCCGGY 1 cut(s) 308
BseBI CCWGG 1 cut(s) 442
BseDI CCNNGG 1 cut(s) 87
BseGI GGATG 1 cut(s) 243
BseXI GCAGC 2 cut(s) 197, 292
BsiSI CCGG 2 cut(s) 89, 309
BslFI GGGAC 1 cut(s) 26
BsmFI GGGAC 1 cut(s) 26
BspLI GGNNCC 1 cut(s) 14
BspMI ACCTGC 1 cut(s) 436
BsrFI RCCGGY 1 cut(s) 308
BssAI RCCGGY 1 cut(s) 308
BssECI CCNNGG 1 cut(s) 87
BssNI GRCGYC 1 cut(s) 251
Bst2UI CCWGG 1 cut(s) 442
Bst4CI ACNGT 2 cut(s) 379, 421
Bst6I CTCTTC 1 cut(s) 402
BstACI GRCGYC 1 cut(s) 251
BstAPI GCANNNNNTGC 1 cut(s) 191
BstF5I GGATG 1 cut(s) 243
BstH2I RGCGCY 1 cut(s) 306
BstHHI GCGC 1 cut(s) 305
BstMWI GCNNNNNNNGC 3 cut(s) 92, 168, 191
BstNI CCWGG 1 cut(s) 442
BstSCI CCNGG 2 cut(s) 87, 440
BstSFI CTRYAG 1 cut(s) 378
BstV1I GCAGC 2 cut(s) 197, 292
BtsCI GGATG 1 cut(s) 243
BtsIMutI CAGTG 1 cut(s) 426
BveI ACCTGC 1 cut(s) 436
CfoI GCGC 1 cut(s) 305
Cfr10I RCCGGY 1 cut(s) 308
Cfr13I GGNCC 1 cut(s) 13
CseI GACGC 1 cut(s) 259
Csp6I GTAC 5 cut(s) 53, 125, 262, 280, 417
CviAII CATG 2 cut(s) 83, 432
CviJI RGCY 6 cut(s) 23, 144, 239, 358, 363, 387
CviKI_1 RGCY 6 cut(s) 23, 144, 239, 358, 363, 387
CviQI GTAC 5 cut(s) 53, 125, 262, 280, 417
Eam1104I CTCTTC 1 cut(s) 402
EarI CTCTTC 1 cut(s) 402
Eco47I GGWCC 1 cut(s) 13
EcoO109I RGGNCCY 1 cut(s) 13
EcoRII CCWGG 1 cut(s) 440
EcoT22I ATGCAT 1 cut(s) 215
FaeI CATG 2 cut(s) 86, 435
FaqI GGGAC 1 cut(s) 26
FatI CATG 2 cut(s) 82, 431
FblI GTMKAC 1 cut(s) 33
Fnu4HI GCNGC 2 cut(s) 186, 306
FokI GGATG 1 cut(s) 230
Fsp4HI GCNGC 2 cut(s) 186, 306
FspBI CTAG 1 cut(s) 56
GlaI GCGC 1 cut(s) 304
GluI GCNGC 2 cut(s) 186, 306
HaeII RGCGCY 1 cut(s) 306
HapII CCGG 2 cut(s) 89, 309
HgaI GACGC 1 cut(s) 259
HhaI GCGC 1 cut(s) 305
Hin1I GRCGYC 1 cut(s) 251
Hin1II CATG 2 cut(s) 86, 435
Hin6I GCGC 1 cut(s) 303
HinP1I GCGC 1 cut(s) 303
HindIII AAGCTT 1 cut(s) 356
HinfI GANTC 1 cut(s) 437
HpaII CCGG 2 cut(s) 89, 309
HphI GGTGA 1 cut(s) 341
Hpy166II GTNNAC 1 cut(s) 34
Hpy188I TCNGA 1 cut(s) 408
Hpy188III TCNNGA 1 cut(s) 247
Hpy8I GTNNAC 1 cut(s) 34
Hpy99I CGWCG 1 cut(s) 167
HpyCH4III ACNGT 2 cut(s) 379, 421
HpyCH4V TGCA 4 cut(s) 185, 194, 213, 431
HpyF10VI GCNNNNNNNGC 3 cut(s) 92, 168, 191
Hsp92I GRCGYC 1 cut(s) 251
Hsp92II CATG 2 cut(s) 86, 435
HspAI GCGC 1 cut(s) 303
LpnPI CCDG 7 cut(s) 29, 102, 260, 322, 349, 427, 441
Lsp1109I GCAGC 2 cut(s) 197, 292
LweI GCATC 2 cut(s) 203, 222
MaeI CTAG 1 cut(s) 56
MaeIII GTNAC 2 cut(s) 3, 329
MboII GAAGA 2 cut(s) 192, 389
MfeI CAATTG 1 cut(s) 228
MluCI AATT 3 cut(s) 70, 228, 336
MnlI CCTC 4 cut(s) 119, 138, 405, 411
Mph1103I ATGCAT 1 cut(s) 215
MseI TTAA 1 cut(s) 335
MspI CCGG 2 cut(s) 89, 309
MspR9I CCNGG 2 cut(s) 89, 442
MunI CAATTG 1 cut(s) 228
MvaI CCWGG 1 cut(s) 442
MwoI GCNNNNNNNGC 3 cut(s) 92, 168, 191
NciI CCSGG 1 cut(s) 89
NlaIII CATG 2 cut(s) 86, 435
NlaIV GGNNCC 1 cut(s) 14
NmuCI GTSAC 2 cut(s) 3, 329
NsiI ATGCAT 1 cut(s) 215
PfeI GAWTC 1 cut(s) 437
PkrI GCNGC 2 cut(s) 187, 307
PpuMI RGGWCCY 1 cut(s) 13
Psp5II RGGWCCY 1 cut(s) 13
Psp6I CCWGG 1 cut(s) 440
PspGI CCWGG 1 cut(s) 440
PspN4I GGNNCC 1 cut(s) 14
PspPI GGNCC 1 cut(s) 13
PspPPI RGGWCCY 1 cut(s) 13
RsaI GTAC 5 cut(s) 54, 126, 263, 281, 418
RsaNI GTAC 5 cut(s) 53, 125, 262, 280, 417
SaqAI TTAA 1 cut(s) 335
SatI GCNGC 2 cut(s) 186, 306
Sau96I GGNCC 1 cut(s) 13
ScaI AGTACT 1 cut(s) 54
ScrFI CCNGG 2 cut(s) 89, 442
SetI ASST 7 cut(s) 18, 25, 130, 177, 331, 360, 430
SfaNI GCATC 2 cut(s) 203, 222
SfcI CTRYAG 1 cut(s) 378
SinI GGWCC 1 cut(s) 13
Sse9I AATT 3 cut(s) 70, 228, 336
SspMI CTAG 1 cut(s) 56
StyD4I CCNGG 2 cut(s) 87, 440
TaaI ACNGT 2 cut(s) 379, 421
TasI AATT 3 cut(s) 70, 228, 336
TatI WGTACW 4 cut(s) 52, 261, 279, 416
TfiI GAWTC 1 cut(s) 437
Tru1I TTAA 1 cut(s) 335
Tru9I TTAA 1 cut(s) 335
TscAI CASTG 1 cut(s) 426
TseFI GTSAC 2 cut(s) 3, 329
TseI GCWGC 2 cut(s) 185, 305
Tsp45I GTSAC 2 cut(s) 3, 329
TspDTI ATGAA 1 cut(s) 71
TspRI CASTG 1 cut(s) 426
VpaK11BI GGWCC 1 cut(s) 13
XmiI GTMKAC 1 cut(s) 33
XspI CTAG 1 cut(s) 56
ZrmI AGTACT 1 cut(s) 54
Zsp2I ATGCAT 1 cut(s) 215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.