RLG00000012765

Membrane protein of ER body-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0002486
Physical Location & Seq
Reverse (-)
22169202 .. 22171378
2177 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012765

Sequence Viewer

Length: 1218 bp
ATGGAAGCAACATTAGAAAGGCTGCCTCGCCAAAACAGCTACACAGACATTACCACCAATAATGGTGGGAATAGAAATGTTGTCTATTCCTTGGAAGAAGGGAAGGGAAGCCCAAGAAATATTGGTACTCACATGGTCTCTGATAAAAGAGCAGGTACTGAATTGCTATATGGGGCTACATCCAGTAGTGGACATGGAATAGTGACTGCTGGAGTAGAGAGCGTACGTTTTGAAAAGAGTTTTGAGACCAAGCACCAAGACGAGGATACTAGGAAGCTGAAGGATCTTGGCTCTTCATCCTCCAAGCAGTCCACTGAGGAAAATGGAATGGCTAAGAGAAAAATTAAGTTGAGAGATCTTGAAGGGGTGGTTGAGAAGACGGCTGCGCATGAATTCTACTGTCCTAATTGCAAGGCTTGCATCACCGAGGTTATAGTCCGCGATGCACCTACTGAGCCAGATGAACCAGTCACATGCACTTCGTGCTTTGCTATCCTATTTCCTCCAGGTACTTTTTCATGTTATGTTATGTTTGTTCTCACATTTGGTGTCTCGATCTCTTTATATTGTGTTGATACAAAACCTACAGGGAGCACAACAGTTATTACTGTGACACCAGTACCAACGTCGACAGGCATCATCTACGGTGGTTTAAATGAAGCAATTGCAAGCTTCAGCATTGTGGCATCCGCAGCTAGTACTGACACTGCCACATTGAACATTTTCGTTTTGGCATTGGCAAACTTGCTGGGTGGTCTTTTCATCATCGGACACAATCTTTGGGACCTGAAAGCTGATCAAGTAGACAATAAATACAAAGAAGTACTAGGGAACAAAGATAATTTCTTTTTTCATGCCCCTGTTGCCATACTCTCTTTCCTCATTTTCGGGTTAGTACCTCCTTTGGTCTATGGCTTCTCGTTCCCTCAGAGCGACGACAGCGACCTAAAGATTGCAGCAGTTGCAGCATCTTCTCTTTTATGCATCATACTTCTCGCAATTGGAAAGGCTTACATCCAGACTCCACCAAAGTACATCAAAACTGTGCTGTACTATGTCATCATGGGGGTTGGGGCTGCCGGTCTTTCTTACTTAGTAGGTGACTTAATTGACAAACTTGTAGAGAAGCTTGGGTGGTTCAGCTCAACTGTGTCTGGCTCTCTGCCTCTTCCTCATCAGACTATGAGTACAGTGAAGCCTGGATGGGAATCCTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VIT1

Protein Analysis

406

Amino Acids

43.73

Weight (kDa)

5.71

Isoelectric Point (pI)

35.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VIT1 PF01988 285 - 367 4.8e-08 VIT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 387
Acc36I ACCTGC 1 cut(s) 143
AccI GTMKAC 2 cut(s) 629, 804
AccII CGCG 1 cut(s) 441
AciI CCGC 2 cut(s) 439, 690
AclWI GGATC 1 cut(s) 291
AcsI RAATTY 1 cut(s) 392
AcuI CTGAAG 2 cut(s) 299, 658
AdeI CACNNNGTG 1 cut(s) 483
AfiI CCNNNNNNNGG 1 cut(s) 262
AgsI TTSAA 3 cut(s) 233, 362, 718
AjnI CCWGG 3 cut(s) 505, 1198, 1211
AluBI AGCT 7 cut(s) 39, 277, 672, 695, 794, 1129, 1143
AluI AGCT 7 cut(s) 39, 277, 672, 695, 794, 1129, 1143
Alw21I GWGCWC 1 cut(s) 596
Alw26I GTCTC 3 cut(s) 142, 239, 556
AlwI GGATC 1 cut(s) 291
AlwNI CAGNNNCTG 1 cut(s) 158
ApeKI GCWGC 6 cut(s) 22, 383, 692, 956, 965, 1076
ApoI RAATTY 1 cut(s) 392
ArsI GACNNNNNNTTYG 2 cut(s) 762, 794
Asp700I GAANNNNTTC 1 cut(s) 722
AspLEI GCGC 1 cut(s) 388
AspS9I GGNCC 1 cut(s) 784
AsuHPI GGTGA 2 cut(s) 415, 1112
AvaII GGWCC 1 cut(s) 784
BaeI ACNNNNGTAYC 2 cut(s) 603, 636
BbsI GAAGAC 1 cut(s) 383
Bbv12I GWGCWC 1 cut(s) 596
BbvI GCAGC 6 cut(s) 9, 370, 704, 968, 977, 1063
BccI CCATC 1 cut(s) 1197
BceAI ACGGC 1 cut(s) 396
BciT130I CCWGG 3 cut(s) 507, 1200, 1213
BciVI GTATCC 1 cut(s) 259
BclI TGATCA 1 cut(s) 796
BcoDI GTCTC 3 cut(s) 142, 239, 556
BfaI CTAG 3 cut(s) 270, 696, 827
BfmI CTRYAG 1 cut(s) 585
BfuAI ACCTGC 1 cut(s) 143
BfuI GTATCC 1 cut(s) 259
BglII AGATCT 1 cut(s) 355
BisI GCNGC 6 cut(s) 23, 384, 693, 957, 966, 1077
BlsI GCNGC 6 cut(s) 24, 385, 694, 958, 967, 1078
BmcAI AGTACT 2 cut(s) 700, 825
Bme1390I CCNGG 3 cut(s) 507, 1200, 1213
Bme18I GGWCC 1 cut(s) 784
BmgT120I GGNCC 1 cut(s) 784
BmiI GGNNCC 1 cut(s) 785
BmrFI CCNGG 3 cut(s) 507, 1200, 1213
BmsI GCATC 6 cut(s) 429, 433, 645, 695, 977, 993
BpiI GAAGAC 1 cut(s) 383
BpmI CTGGAG 2 cut(s) 231, 489
BsaBI GATNNNNATC 1 cut(s) 1207
BsaI GGTCTC 2 cut(s) 142, 239
BsaJI CCNNGG 2 cut(s) 90, 426
Bsc4I CCNNNNNNNGG 1 cut(s) 262
Bse118I RCCGGY 1 cut(s) 1079
Bse1I ACTGG 3 cut(s) 183, 467, 617
Bse8I GATNNNNATC 1 cut(s) 1207
BseBI CCWGG 3 cut(s) 507, 1200, 1213
BseDI CCNNGG 2 cut(s) 90, 426
BseGI GGATG 5 cut(s) 179, 296, 686, 1014, 1208
BseJI GATNNNNATC 1 cut(s) 1207
BseLI CCNNNNNNNGG 1 cut(s) 262
BseMII CTCAG 3 cut(s) 306, 444, 941
BseNI ACTGG 3 cut(s) 183, 467, 617
BseXI GCAGC 6 cut(s) 9, 370, 704, 968, 977, 1063
BseYI CCCAGC 1 cut(s) 748
Bsh1236I CGCG 1 cut(s) 441
BsiHKAI GWGCWC 1 cut(s) 596
BsiSI CCGG 1 cut(s) 1080
BsiWI CGTACG 1 cut(s) 223
BslFI GGGAC 1 cut(s) 797
BslI CCNNNNNNNGG 1 cut(s) 262
BsmAI GTCTC 3 cut(s) 142, 239, 556
BsmFI GGGAC 1 cut(s) 797
Bso31I GGTCTC 2 cut(s) 142, 239
Bsp1286I GDGCHC 1 cut(s) 596
Bsp143I GATC 4 cut(s) 283, 355, 555, 796
BspACI CCGC 2 cut(s) 439, 690
BspCNI CTCAG 3 cut(s) 307, 445, 940
BspFNI CGCG 1 cut(s) 441
BspLI GGNNCC 1 cut(s) 785
BspMI ACCTGC 1 cut(s) 143
BspPI GGATC 1 cut(s) 291
BspQI GCTCTTC 1 cut(s) 298
BspTNI GGTCTC 2 cut(s) 142, 239
BsrFI RCCGGY 1 cut(s) 1079
BsrI ACTGG 3 cut(s) 183, 467, 617
BssAI RCCGGY 1 cut(s) 1079
BssECI CCNNGG 2 cut(s) 90, 426
BssMI GATC 4 cut(s) 283, 355, 555, 796
BssT1I CCWWGG 1 cut(s) 90
Bst2UI CCWGG 3 cut(s) 507, 1200, 1213
Bst4CI ACNGT 7 cut(s) 401, 601, 610, 647, 1045, 1150, 1192
Bst6I CTCTTC 2 cut(s) 298, 1173
BstAPI GCANNNNNTGC 3 cut(s) 417, 483, 962
BstC8I GCNNGC 2 cut(s) 418, 670
BstDEI CTNAG 5 cut(s) 315, 333, 453, 927, 1093
BstF5I GGATG 5 cut(s) 179, 296, 686, 1014, 1208
BstFNI CGCG 1 cut(s) 441
BstHHI GCGC 1 cut(s) 388
BstKTI GATC 4 cut(s) 286, 358, 558, 799
BstMAI GTCTC 3 cut(s) 142, 239, 556
BstMBI GATC 4 cut(s) 283, 355, 555, 796
BstMWI GCNNNNNNNGC 8 cut(s) 36, 417, 483, 692, 863, 939, 962, 965
BstNI CCWGG 3 cut(s) 507, 1200, 1213
BstNSI RCATGY 1 cut(s) 477
BstSCI CCNGG 3 cut(s) 505, 1198, 1211
BstSFI CTRYAG 1 cut(s) 585
BstUI CGCG 1 cut(s) 441
BstV1I GCAGC 6 cut(s) 9, 370, 704, 968, 977, 1063
BstV2I GAAGAC 1 cut(s) 383
BstX2I RGATCY 2 cut(s) 283, 355
BstYI RGATCY 2 cut(s) 283, 355
BsuI GTATCC 1 cut(s) 259
BtgZI GCGATG 1 cut(s) 456
BtsCI GGATG 5 cut(s) 179, 296, 686, 1014, 1208
BtsI GCAGTG 1 cut(s) 705
BtsIMutI CAGTG 3 cut(s) 312, 705, 1197
BveI ACCTGC 1 cut(s) 143
Cac8I GCNNGC 2 cut(s) 418, 670
CaiI CAGNNNCTG 1 cut(s) 158
CfoI GCGC 1 cut(s) 388
Cfr10I RCCGGY 1 cut(s) 1079
Cfr13I GGNCC 1 cut(s) 784
CspCI CAANNNNNGTGG 2 cut(s) 46, 81
CviAII CATG 7 cut(s) 133, 194, 389, 474, 519, 854, 1063
DdeI CTNAG 5 cut(s) 315, 333, 453, 927, 1093
DpnI GATC 4 cut(s) 285, 357, 557, 798
DpnII GATC 4 cut(s) 283, 355, 555, 796
DraI TTTAAA 1 cut(s) 654
DraIII CACNNNGTG 1 cut(s) 483
Eam1104I CTCTTC 2 cut(s) 298, 1173
EarI CTCTTC 2 cut(s) 298, 1173
Eco130I CCWWGG 1 cut(s) 90
Eco31I GGTCTC 2 cut(s) 142, 239
Eco47I GGWCC 1 cut(s) 784
Eco57I CTGAAG 2 cut(s) 299, 658
EcoO109I RGGNCCY 1 cut(s) 784
EcoRI GAATTC 1 cut(s) 392
EcoRII CCWGG 3 cut(s) 505, 1198, 1211
EcoT14I CCWWGG 1 cut(s) 90
EcoT22I ATGCAT 1 cut(s) 986
ErhI CCWWGG 1 cut(s) 90
FaeI CATG 7 cut(s) 136, 197, 392, 477, 522, 857, 1066
FaqI GGGAC 1 cut(s) 797
FatI CATG 7 cut(s) 132, 193, 388, 473, 518, 853, 1062
FbaI TGATCA 1 cut(s) 796
FblI GTMKAC 2 cut(s) 629, 804
Fnu4HI GCNGC 6 cut(s) 23, 384, 693, 957, 966, 1077
FokI GGATG 4 cut(s) 166, 283, 673, 1001
Fsp4HI GCNGC 6 cut(s) 23, 384, 693, 957, 966, 1077
FspBI CTAG 3 cut(s) 270, 696, 827
FspI TGCGCA 1 cut(s) 387
GlaI GCGC 1 cut(s) 387
GluI GCNGC 6 cut(s) 23, 384, 693, 957, 966, 1077
GsaI CCCAGC 1 cut(s) 752
GsuI CTGGAG 2 cut(s) 231, 489
HapII CCGG 1 cut(s) 1080
HhaI GCGC 1 cut(s) 388
Hin1II CATG 7 cut(s) 136, 197, 392, 477, 522, 857, 1066
Hin6I GCGC 1 cut(s) 386
HinP1I GCGC 1 cut(s) 386
HincII GTYRAC 1 cut(s) 630
HindII GTYRAC 1 cut(s) 630
HindIII AAGCTT 2 cut(s) 670, 1127
HinfI GANTC 2 cut(s) 1021, 1208
HpaII CCGG 1 cut(s) 1080
HphI GGTGA 2 cut(s) 415, 1112
Hpy166II GTNNAC 4 cut(s) 191, 312, 630, 805
Hpy188I TCNGA 4 cut(s) 142, 770, 930, 1179
Hpy188III TCNNGA 3 cut(s) 359, 553, 1018
Hpy8I GTNNAC 4 cut(s) 191, 312, 630, 805
Hpy99I CGWCG 2 cut(s) 631, 938
HpyAV CCTTC 4 cut(s) 92, 97, 274, 356
HpyCH4III ACNGT 7 cut(s) 401, 601, 610, 647, 1045, 1150, 1192
HpyCH4IV ACGT 2 cut(s) 226, 626
HpyCH4V TGCA 8 cut(s) 411, 420, 446, 477, 668, 956, 965, 984
HpyF10VI GCNNNNNNNGC 8 cut(s) 36, 417, 483, 692, 863, 939, 962, 965
HpyF3I CTNAG 5 cut(s) 315, 333, 453, 927, 1093
HpySE526I ACGT 2 cut(s) 226, 626
Hsp92II CATG 7 cut(s) 136, 197, 392, 477, 522, 857, 1066
HspAI GCGC 1 cut(s) 386
Ksp22I TGATCA 1 cut(s) 796
Kzo9I GATC 4 cut(s) 283, 355, 555, 796
LguI GCTCTTC 1 cut(s) 298
LmnI GCTCC 1 cut(s) 591
Lsp1109I GCAGC 6 cut(s) 9, 370, 704, 968, 977, 1063
LweI GCATC 6 cut(s) 429, 433, 645, 695, 977, 993
MaeI CTAG 3 cut(s) 270, 696, 827
MaeII ACGT 2 cut(s) 226, 626
MaeIII GTNAC 4 cut(s) 202, 469, 610, 1100
MalI GATC 4 cut(s) 285, 357, 557, 798
MboI GATC 4 cut(s) 283, 355, 555, 796
MboII GAAGA 5 cut(s) 107, 285, 388, 963, 1160
MfeI CAATTG 2 cut(s) 663, 999
MflI RGATCY 2 cut(s) 283, 355
MhlI GDGCHC 1 cut(s) 596
MluCI AATT 8 cut(s) 161, 342, 392, 406, 663, 841, 999, 1107
MlyI GAGTC 1 cut(s) 1015
Mph1103I ATGCAT 1 cut(s) 986
MroXI GAANNNNTTC 1 cut(s) 722
MseI TTAA 3 cut(s) 345, 653, 1106
MspI CCGG 1 cut(s) 1080
MspR9I CCNGG 3 cut(s) 507, 1200, 1213
MunI CAATTG 2 cut(s) 663, 999
MvaI CCWGG 3 cut(s) 507, 1200, 1213
MvnI CGCG 1 cut(s) 441
MwoI GCNNNNNNNGC 8 cut(s) 36, 417, 483, 692, 863, 939, 962, 965
NdeII GATC 4 cut(s) 283, 355, 555, 796
NlaIII CATG 7 cut(s) 136, 197, 392, 477, 522, 857, 1066
NlaIV GGNNCC 1 cut(s) 785
NmuCI GTSAC 4 cut(s) 202, 469, 610, 1100
NsbI TGCGCA 1 cut(s) 387
NsiI ATGCAT 1 cut(s) 986
NspI RCATGY 1 cut(s) 477
PciSI GCTCTTC 1 cut(s) 298
PdmI GAANNNNTTC 1 cut(s) 722
PfeI GAWTC 1 cut(s) 1208
Pfl23II CGTACG 1 cut(s) 223
PkrI GCNGC 6 cut(s) 24, 385, 694, 958, 967, 1078
PleI GAGTC 1 cut(s) 1015
PpsI GAGTC 1 cut(s) 1015
PpuMI RGGWCCY 1 cut(s) 784
Psp5II RGGWCCY 1 cut(s) 784
Psp6I CCWGG 3 cut(s) 505, 1198, 1211
PspFI CCCAGC 1 cut(s) 748
PspGI CCWGG 3 cut(s) 505, 1198, 1211
PspLI CGTACG 1 cut(s) 223
PspN4I GGNNCC 1 cut(s) 785
PspPI GGNCC 1 cut(s) 784
PspPPI RGGWCCY 1 cut(s) 784
PstNI CAGNNNCTG 1 cut(s) 158
PsuI RGATCY 2 cut(s) 283, 355
SalI GTCGAC 1 cut(s) 628
SapI GCTCTTC 1 cut(s) 298
SaqAI TTAA 3 cut(s) 345, 653, 1106
SatI GCNGC 6 cut(s) 23, 384, 693, 957, 966, 1077
Sau3AI GATC 4 cut(s) 283, 355, 555, 796
Sau96I GGNCC 1 cut(s) 784
ScaI AGTACT 2 cut(s) 700, 825
SchI GAGTC 1 cut(s) 1015
ScrFI CCNGG 3 cut(s) 507, 1200, 1213
SduI GDGCHC 1 cut(s) 596
SfaNI GCATC 6 cut(s) 429, 433, 645, 695, 977, 993
SfcI CTRYAG 1 cut(s) 585
SinI GGWCC 1 cut(s) 784
Sse9I AATT 8 cut(s) 161, 342, 392, 406, 663, 841, 999, 1107
SsiI CCGC 2 cut(s) 439, 690
SspI AATATT 1 cut(s) 121
SspMI CTAG 3 cut(s) 270, 696, 827
StyD4I CCNGG 3 cut(s) 505, 1198, 1211
StyI CCWWGG 1 cut(s) 90
TaaI ACNGT 7 cut(s) 401, 601, 610, 647, 1045, 1150, 1192
TaiI ACGT 2 cut(s) 229, 629
TaqI TCGA 2 cut(s) 554, 629
TasI AATT 8 cut(s) 161, 342, 392, 406, 663, 841, 999, 1107
TatI WGTACW 5 cut(s) 698, 823, 1032, 1050, 1187
TfiI GAWTC 1 cut(s) 1208
Tru1I TTAA 3 cut(s) 345, 653, 1106
Tru9I TTAA 3 cut(s) 345, 653, 1106
TscAI CASTG 3 cut(s) 319, 712, 1197
TseFI GTSAC 4 cut(s) 202, 469, 610, 1100
TseI GCWGC 6 cut(s) 22, 383, 692, 956, 965, 1076
Tsp45I GTSAC 4 cut(s) 202, 469, 610, 1100
TspDTI ATGAA 7 cut(s) 285, 405, 477, 507, 672, 751, 842
TspRI CASTG 3 cut(s) 319, 712, 1197
VpaK11BI GGWCC 1 cut(s) 784
XapI RAATTY 1 cut(s) 392
XceI RCATGY 1 cut(s) 477
XmiI GTMKAC 2 cut(s) 629, 804
XmnI GAANNNNTTC 1 cut(s) 722
XspI CTAG 3 cut(s) 270, 696, 827
ZrmI AGTACT 2 cut(s) 700, 825
Zsp2I ATGCAT 1 cut(s) 986
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.