Rh6AG274000

Membrane protein of ER body-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0002486
Physical Location & Seq
Reverse (-)
46823354 .. 46826456
3103 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG274000.1

Sequence Viewer

Length: 1281 bp
ATGGAAGCACCATTAGAAAAGCTGCCTCGCCAAAACAGCAACACAGACATTACCACCAATAATGGTGGGAATGTTGTCTATTCCTTCGAAGAAGGGAAGAGAAGCCCAAGAAATGGTACTGAATTGCTATACGGAGCTACATCCAATAGTGGACATGGAATAGTGACTGTTGGAGTAGAGAGCGTACGTTTTCAAAAGAGTGTTGAGACCAAGCACCAAGACGAGGATACTAGGAAGTTGAAGGATCTTGGCTCTTCATCCTCCGAGCAGACGGAGCAGTCCCCTGAGGAAAATGGAGTGGCTAAGCATCGAAAAATTAAGTTGAGAGATCTTGAAGGGGTGTTTGAGAAGACGGCTGCGCATGAATTCTACTGTCCTAATTGCAATGCTTGCATCACCGAGGTTATAGTCCGCGATAGAGAAGCAAAACCTCCTACCCCACCACTTATTGAGCCAGATGAACCAAAACCTCCTACCAAACCACTTATTGAGCCAGAGCCAGATGAACCAAAACCTCCTACTCCACCGCTTATTGTGCCAGATGAACCGGCCGTCAGATGCACTTCGTGCTTTGGTCTCCTATTTCCTGCAGAACCTACACAGGGCACAACAGTTATTACTATCACACCGCCGGAACAAACGCCGGCAGGGTCTGAATTACCACGAGATGCTAATAAGATGTTAGAAATCCTCAAAAGCATCGTTTACGGTGGTTTAAATGAAGCAATTGCAAGCTTAAGCATTGTGGCATCTGCAGCTAGTACTGATACTGCCACATTGAACATTTTCGTTTTGGCACTCGCAAACTTGCTGGGTGGTCTTTTCGTCATCGGACACAATCTCTGGGACCTGAAAGCTGATCAAGTAGACAGTAAATACAAAGAAGTACTAGGGAACAAAGATAATTTCTTTTTTCATGTCCCGGTTGCCATACTCTCGTTCCTCATTTTCGGGTTAGTACCTCCTTTGGTTTATGGCTTCTCGTTCTCTCAGAGCGACGACAGCGACCTAAAGATTGCAGCAGTTGCAGCATCTTCTATTTTATGCATCATACTTCTCGCAATTGGAAAGGCTTACATCCAGACGCCACCAGAGTACATCAAAACTGTGCTGTACTATATCGTCATAGGGGTTGGCGCCGCCGGTGTTTCTTACTTGGTAGGTGACTTAATTGACAAACTTGTAGAGAAGCTTGGCTGGTTCAACTCAACTGTAGCTGGCTCTCTGCCTCTTCCTCATCTGACTATGAGTACAGTGAAACCTGCATGGGAATCCTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VIT1

Protein Analysis

426

Amino Acids

46.18

Weight (kDa)

5.02

Isoelectric Point (pI)

42.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VIT1 PF01988 311 - 388 1.6e-06 VIT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 277
Acc16I TGCGCA 1 cut(s) 360
Acc36I ACCTGC 1 cut(s) 1270
AccB1I GGYRCC 1 cut(s) 1136
AccB7I CCANNNNNTGG 1 cut(s) 113
AccI GTMKAC 1 cut(s) 867
AccII CGCG 1 cut(s) 414
AciI CCGC 4 cut(s) 412, 527, 629, 1140
AclWI GGATC 1 cut(s) 252
AcoI YGGCCR 1 cut(s) 549
AcsI RAATTY 1 cut(s) 365
AcyI GRCGYC 2 cut(s) 1085, 1137
AdeI CACNNNGTG 1 cut(s) 567
AfaI GTAC 8 cut(s) 118, 186, 763, 888, 960, 1097, 1115, 1252
AfiI CCNNNNNNNGG 3 cut(s) 113, 223, 602
AflII CTTAAG 1 cut(s) 736
AgsI TTSAA 5 cut(s) 194, 241, 335, 781, 1204
AjnI CCWGG 1 cut(s) 1274
AluBI AGCT 7 cut(s) 22, 137, 735, 758, 857, 1192, 1217
AluI AGCT 7 cut(s) 22, 137, 735, 758, 857, 1192, 1217
Alw26I GTCTC 2 cut(s) 200, 581
AlwI GGATC 1 cut(s) 252
AlwNI CAGNNNCTG 1 cut(s) 653
AoxI GGCC 1 cut(s) 549
ApeKI GCWGC 5 cut(s) 22, 356, 755, 1019, 1028
ApoI RAATTY 1 cut(s) 365
Asp700I GAANNNNTTC 1 cut(s) 785
AspLEI GCGC 2 cut(s) 361, 1139
AspS9I GGNCC 1 cut(s) 847
AsuC2I CCSGG 1 cut(s) 923
AsuHPI GGTGA 2 cut(s) 388, 1175
AsuII TTCGAA 1 cut(s) 87
AvaII GGWCC 1 cut(s) 847
AxyI CCTNAGG 1 cut(s) 285
BaeGI GKGCMC 1 cut(s) 608
BanI GGYRCC 1 cut(s) 1136
BauI CACGAG 1 cut(s) 663
BbsI GAAGAC 1 cut(s) 356
BbvI GCAGC 5 cut(s) 9, 343, 767, 1031, 1040
BceAI ACGGC 2 cut(s) 369, 536
BciT130I CCWGG 1 cut(s) 1276
BciVI GTATCC 1 cut(s) 220
BclI TGATCA 1 cut(s) 859
BcnI CCSGG 1 cut(s) 923
BcoDI GTCTC 2 cut(s) 200, 581
BfaI CTAG 3 cut(s) 231, 759, 890
BfmI CTRYAG 3 cut(s) 588, 753, 1212
BfoI RGCGCY 1 cut(s) 1140
BfrI CTTAAG 1 cut(s) 736
BfuAI ACCTGC 1 cut(s) 1270
BfuI GTATCC 1 cut(s) 220
BglII AGATCT 1 cut(s) 328
BisI GCNGC 6 cut(s) 23, 357, 756, 1020, 1029, 1140
BlpI GCTNAGC 1 cut(s) 303
BlsI GCNGC 6 cut(s) 24, 358, 757, 1021, 1030, 1141
BmcAI AGTACT 2 cut(s) 763, 888
Bme1390I CCNGG 2 cut(s) 923, 1276
Bme18I GGWCC 1 cut(s) 847
BmgT120I GGNCC 1 cut(s) 847
BmiI GGNNCC 2 cut(s) 848, 1138
BmrFI CCNGG 2 cut(s) 923, 1276
BmsI GCATC 8 cut(s) 316, 402, 548, 658, 708, 758, 1040, 1056
BpiI GAAGAC 1 cut(s) 356
Bpu1102I GCTNAGC 1 cut(s) 303
Bpu14I TTCGAA 1 cut(s) 87
BpuMI CCSGG 1 cut(s) 923
BsaHI GRCGYC 2 cut(s) 1085, 1137
BsaI GGTCTC 2 cut(s) 200, 581
BsaJI CCNNGG 1 cut(s) 399
Bsc4I CCNNNNNNNGG 3 cut(s) 113, 223, 602
Bse118I RCCGGY 3 cut(s) 547, 643, 1142
Bse21I CCTNAGG 1 cut(s) 285
Bse3DI GCAATG 1 cut(s) 391
BseBI CCWGG 1 cut(s) 1276
BseDI CCNNGG 1 cut(s) 399
BseGI GGATG 3 cut(s) 140, 257, 1077
BseLI CCNNNNNNNGG 3 cut(s) 113, 223, 602
BseMI GCAATG 1 cut(s) 391
BseMII CTCAG 2 cut(s) 276, 1004
BseSI GKGCMC 1 cut(s) 608
BseX3I CGGCCG 1 cut(s) 549
BseXI GCAGC 5 cut(s) 9, 343, 767, 1031, 1040
BseYI CCCAGC 1 cut(s) 811
Bsh1236I CGCG 1 cut(s) 414
Bsh1285I CGRYCG 1 cut(s) 552
BshFI GGCC 1 cut(s) 551
BshNI GGYRCC 1 cut(s) 1136
BsiEI CGRYCG 1 cut(s) 552
BsiSI CCGG 5 cut(s) 548, 632, 644, 923, 1143
BsiWI CGTACG 1 cut(s) 184
BslFI GGGAC 3 cut(s) 265, 860, 905
BslI CCNNNNNNNGG 3 cut(s) 113, 223, 602
BsmAI GTCTC 2 cut(s) 200, 581
BsmFI GGGAC 3 cut(s) 265, 860, 905
BsnI GGCC 1 cut(s) 551
Bso31I GGTCTC 2 cut(s) 200, 581
Bsp119I TTCGAA 1 cut(s) 87
Bsp1286I GDGCHC 1 cut(s) 608
Bsp143I GATC 3 cut(s) 244, 328, 859
Bsp1720I GCTNAGC 1 cut(s) 303
BspACI CCGC 4 cut(s) 412, 527, 629, 1140
BspANI GGCC 1 cut(s) 551
BspCNI CTCAG 2 cut(s) 277, 1003
BspFNI CGCG 1 cut(s) 414
BspLI GGNNCC 2 cut(s) 848, 1138
BspMAI CTGCAG 2 cut(s) 592, 757
BspMI ACCTGC 1 cut(s) 1270
BspPI GGATC 1 cut(s) 252
BspQI GCTCTTC 1 cut(s) 259
BspT104I TTCGAA 1 cut(s) 87
BspT107I GGYRCC 1 cut(s) 1136
BspTI CTTAAG 1 cut(s) 736
BspTNI GGTCTC 2 cut(s) 200, 581
BsrDI GCAATG 1 cut(s) 391
BsrFI RCCGGY 3 cut(s) 547, 643, 1142
BssAI RCCGGY 3 cut(s) 547, 643, 1142
BssECI CCNNGG 1 cut(s) 399
BssMI GATC 3 cut(s) 244, 328, 859
BssNI GRCGYC 2 cut(s) 1085, 1137
BssSI CACGAG 1 cut(s) 663
Bst2BI CACGAG 1 cut(s) 663
Bst2UI CCWGG 1 cut(s) 1276
Bst4CI ACNGT 8 cut(s) 169, 374, 613, 710, 872, 1108, 1213, 1255
Bst6I CTCTTC 3 cut(s) 92, 259, 1236
BstACI GRCGYC 2 cut(s) 1085, 1137
BstAFI CTTAAG 1 cut(s) 736
BstAPI GCANNNNNTGC 3 cut(s) 390, 567, 1025
BstBI TTCGAA 1 cut(s) 87
BstC8I GCNNGC 4 cut(s) 391, 645, 733, 1219
BstDEI CTNAG 3 cut(s) 285, 303, 990
BstF5I GGATG 3 cut(s) 140, 257, 1077
BstFNI CGCG 1 cut(s) 414
BstH2I RGCGCY 1 cut(s) 1140
BstHHI GCGC 2 cut(s) 361, 1139
BstKTI GATC 3 cut(s) 247, 331, 862
BstMAI GTCTC 2 cut(s) 200, 581
BstMBI GATC 3 cut(s) 244, 328, 859
BstMCI CGRYCG 1 cut(s) 552
BstMWI GCNNNNNNNGC 9 cut(s) 36, 274, 390, 535, 567, 755, 1002, 1025, 1028
BstNI CCWGG 1 cut(s) 1276
BstSCI CCNGG 2 cut(s) 921, 1274
BstSFI CTRYAG 3 cut(s) 588, 753, 1212
BstSLI GKGCMC 1 cut(s) 608
BstUI CGCG 1 cut(s) 414
BstV1I GCAGC 5 cut(s) 9, 343, 767, 1031, 1040
BstV2I GAAGAC 1 cut(s) 356
BstX2I RGATCY 2 cut(s) 244, 328
BstYI RGATCY 2 cut(s) 244, 328
BstZI CGGCCG 1 cut(s) 549
Bsu36I CCTNAGG 1 cut(s) 285
BsuI GTATCC 1 cut(s) 220
BsuRI GGCC 1 cut(s) 551
BtsCI GGATG 3 cut(s) 140, 257, 1077
BtsIMutI CAGTG 1 cut(s) 1260
BveI ACCTGC 1 cut(s) 1270
Cac8I GCNNGC 4 cut(s) 391, 645, 733, 1219
CaiI CAGNNNCTG 1 cut(s) 653
CfoI GCGC 2 cut(s) 361, 1139
Cfr10I RCCGGY 3 cut(s) 547, 643, 1142
Cfr13I GGNCC 1 cut(s) 847
CseI GACGC 1 cut(s) 1093
Csp6I GTAC 8 cut(s) 117, 185, 762, 887, 959, 1096, 1114, 1251
CspCI CAANNNNNGTGG 2 cut(s) 46, 81
CviAII CATG 4 cut(s) 155, 362, 917, 1266
CviQI GTAC 8 cut(s) 117, 185, 762, 887, 959, 1096, 1114, 1251
DdeI CTNAG 3 cut(s) 285, 303, 990
DinI GGCGCC 1 cut(s) 1138
DpnI GATC 3 cut(s) 246, 330, 861
DpnII GATC 3 cut(s) 244, 328, 859
DraI TTTAAA 1 cut(s) 717
DraIII CACNNNGTG 1 cut(s) 567
DrdI GACNNNNNNGTC 1 cut(s) 277
DseDI GACNNNNNNGTC 1 cut(s) 277
EaeI YGGCCR 1 cut(s) 549
EagI CGGCCG 1 cut(s) 549
Eam1104I CTCTTC 3 cut(s) 92, 259, 1236
EarI CTCTTC 3 cut(s) 92, 259, 1236
EclXI CGGCCG 1 cut(s) 549
Eco31I GGTCTC 2 cut(s) 200, 581
Eco47I GGWCC 1 cut(s) 847
Eco52I CGGCCG 1 cut(s) 549
Eco81I CCTNAGG 1 cut(s) 285
EcoO109I RGGNCCY 1 cut(s) 847
EcoRI GAATTC 1 cut(s) 365
EcoRII CCWGG 1 cut(s) 1274
EcoT22I ATGCAT 1 cut(s) 1049
EgeI GGCGCC 1 cut(s) 1138
EheI GGCGCC 1 cut(s) 1138
FaeI CATG 4 cut(s) 158, 365, 920, 1269
FaqI GGGAC 3 cut(s) 265, 860, 905
FatI CATG 4 cut(s) 154, 361, 916, 1265
FbaI TGATCA 1 cut(s) 859
FblI GTMKAC 1 cut(s) 867
Fnu4HI GCNGC 6 cut(s) 23, 357, 756, 1020, 1029, 1140
FokI GGATG 3 cut(s) 127, 244, 1064
Fsp4HI GCNGC 6 cut(s) 23, 357, 756, 1020, 1029, 1140
FspBI CTAG 3 cut(s) 231, 759, 890
FspI TGCGCA 1 cut(s) 360
GlaI GCGC 2 cut(s) 360, 1138
GluI GCNGC 6 cut(s) 23, 357, 756, 1020, 1029, 1140
GsaI CCCAGC 1 cut(s) 815
HaeII RGCGCY 1 cut(s) 1140
HaeIII GGCC 1 cut(s) 551
HapII CCGG 5 cut(s) 548, 632, 644, 923, 1143
HgaI GACGC 1 cut(s) 1093
HhaI GCGC 2 cut(s) 361, 1139
Hin1I GRCGYC 2 cut(s) 1085, 1137
Hin1II CATG 4 cut(s) 158, 365, 920, 1269
Hin6I GCGC 2 cut(s) 359, 1137
HinP1I GCGC 2 cut(s) 359, 1137
HindIII AAGCTT 2 cut(s) 733, 1190
HinfI GANTC 1 cut(s) 1271
HpaII CCGG 5 cut(s) 548, 632, 644, 923, 1143
HphI GGTGA 2 cut(s) 388, 1175
Hpy166II GTNNAC 3 cut(s) 152, 706, 868
Hpy188I TCNGA 6 cut(s) 265, 557, 655, 833, 993, 1242
Hpy188III TCNNGA 2 cut(s) 332, 1081
Hpy8I GTNNAC 3 cut(s) 152, 706, 868
Hpy99I CGWCG 1 cut(s) 1001
HpyAV CCTTC 4 cut(s) 86, 94, 235, 329
HpyCH4III ACNGT 8 cut(s) 169, 374, 613, 710, 872, 1108, 1213, 1255
HpyCH4IV ACGT 1 cut(s) 187
HpyF10VI GCNNNNNNNGC 9 cut(s) 36, 274, 390, 535, 567, 755, 1002, 1025, 1028
HpyF3I CTNAG 3 cut(s) 285, 303, 990
HpySE526I ACGT 1 cut(s) 187
Hsp92I GRCGYC 2 cut(s) 1085, 1137
Hsp92II CATG 4 cut(s) 158, 365, 920, 1269
HspAI GCGC 2 cut(s) 359, 1137
KasI GGCGCC 1 cut(s) 1136
KroI GCCGGC 1 cut(s) 643
KroNI GCCGGC 1 cut(s) 645
Ksp22I TGATCA 1 cut(s) 859
Kzo9I GATC 3 cut(s) 244, 328, 859
LguI GCTCTTC 1 cut(s) 259
LmnI GCTCC 2 cut(s) 134, 274
Lsp1109I GCAGC 5 cut(s) 9, 343, 767, 1031, 1040
LweI GCATC 8 cut(s) 316, 402, 548, 658, 708, 758, 1040, 1056
MaeI CTAG 3 cut(s) 231, 759, 890
MaeII ACGT 1 cut(s) 187
MaeIII GTNAC 2 cut(s) 163, 1163
MalI GATC 3 cut(s) 246, 330, 861
MboI GATC 3 cut(s) 244, 328, 859
MboII GAAGA 6 cut(s) 101, 109, 246, 361, 1026, 1223
MfeI CAATTG 2 cut(s) 726, 1062
MflI RGATCY 2 cut(s) 244, 328
MhlI GDGCHC 1 cut(s) 608
MluCI AATT 9 cut(s) 122, 315, 365, 379, 656, 726, 904, 1062, 1170
Mly113I GGCGCC 1 cut(s) 1137
MmeI TCCRAC 1 cut(s) 151
Mph1103I ATGCAT 1 cut(s) 1049
MroNI GCCGGC 1 cut(s) 643
MroXI GAANNNNTTC 1 cut(s) 785
MseI TTAA 4 cut(s) 318, 716, 737, 1169
MspCI CTTAAG 1 cut(s) 736
MspI CCGG 5 cut(s) 548, 632, 644, 923, 1143
MspR9I CCNGG 2 cut(s) 923, 1276
MunI CAATTG 2 cut(s) 726, 1062
MvaI CCWGG 1 cut(s) 1276
MvnI CGCG 1 cut(s) 414
MwoI GCNNNNNNNGC 9 cut(s) 36, 274, 390, 535, 567, 755, 1002, 1025, 1028
NaeI GCCGGC 1 cut(s) 645
NarI GGCGCC 1 cut(s) 1137
NciI CCSGG 1 cut(s) 923
NdeII GATC 3 cut(s) 244, 328, 859
NgoMIV GCCGGC 1 cut(s) 643
NlaIII CATG 4 cut(s) 158, 365, 920, 1269
NlaIV GGNNCC 2 cut(s) 848, 1138
NmuCI GTSAC 2 cut(s) 163, 1163
NsbI TGCGCA 1 cut(s) 360
NsiI ATGCAT 1 cut(s) 1049
NspV TTCGAA 1 cut(s) 87
PciSI GCTCTTC 1 cut(s) 259
PdiI GCCGGC 1 cut(s) 645
PdmI GAANNNNTTC 1 cut(s) 785
PfeI GAWTC 1 cut(s) 1271
Pfl23II CGTACG 1 cut(s) 184
PflMI CCANNNNNTGG 1 cut(s) 113
PkrI GCNGC 6 cut(s) 24, 358, 757, 1021, 1030, 1141
PluTI GGCGCC 1 cut(s) 1140
PpuMI RGGWCCY 1 cut(s) 847
Psp5II RGGWCCY 1 cut(s) 847
Psp6I CCWGG 1 cut(s) 1274
PspFI CCCAGC 1 cut(s) 811
PspGI CCWGG 1 cut(s) 1274
PspLI CGTACG 1 cut(s) 184
PspN4I GGNNCC 2 cut(s) 848, 1138
PspPI GGNCC 1 cut(s) 847
PspPPI RGGWCCY 1 cut(s) 847
PstI CTGCAG 2 cut(s) 592, 757
PstNI CAGNNNCTG 1 cut(s) 653
PsuI RGATCY 2 cut(s) 244, 328
RsaI GTAC 8 cut(s) 118, 186, 763, 888, 960, 1097, 1115, 1252
RsaNI GTAC 8 cut(s) 117, 185, 762, 887, 959, 1096, 1114, 1251
SapI GCTCTTC 1 cut(s) 259
SaqAI TTAA 4 cut(s) 318, 716, 737, 1169
SatI GCNGC 6 cut(s) 23, 357, 756, 1020, 1029, 1140
Sau3AI GATC 3 cut(s) 244, 328, 859
Sau96I GGNCC 1 cut(s) 847
ScaI AGTACT 2 cut(s) 763, 888
ScrFI CCNGG 2 cut(s) 923, 1276
SduI GDGCHC 1 cut(s) 608
SfaNI GCATC 8 cut(s) 316, 402, 548, 658, 708, 758, 1040, 1056
SfcI CTRYAG 3 cut(s) 588, 753, 1212
SfoI GGCGCC 1 cut(s) 1138
SfuI TTCGAA 1 cut(s) 87
SgrAI CRCCGGYG 1 cut(s) 1142
SinI GGWCC 1 cut(s) 847
SmlI CTYRAG 1 cut(s) 736
SmoI CTYRAG 1 cut(s) 736
Sse9I AATT 9 cut(s) 122, 315, 365, 379, 656, 726, 904, 1062, 1170
SsiI CCGC 4 cut(s) 412, 527, 629, 1140
SspDI GGCGCC 1 cut(s) 1136
SspMI CTAG 3 cut(s) 231, 759, 890
StyD4I CCNGG 2 cut(s) 921, 1274
TaaI ACNGT 8 cut(s) 169, 374, 613, 710, 872, 1108, 1213, 1255
TaiI ACGT 1 cut(s) 190
TaqI TCGA 2 cut(s) 87, 310
TasI AATT 9 cut(s) 122, 315, 365, 379, 656, 726, 904, 1062, 1170
TatI WGTACW 5 cut(s) 761, 886, 1095, 1113, 1250
TauI GCSGC 1 cut(s) 1142
TfiI GAWTC 1 cut(s) 1271
Tru1I TTAA 4 cut(s) 318, 716, 737, 1169
Tru9I TTAA 4 cut(s) 318, 716, 737, 1169
TscAI CASTG 1 cut(s) 1260
TseFI GTSAC 2 cut(s) 163, 1163
TseI GCWGC 5 cut(s) 22, 356, 755, 1019, 1028
Tsp45I GTSAC 2 cut(s) 163, 1163
TspDTI ATGAA 7 cut(s) 246, 378, 474, 519, 558, 735, 905
TspGWI ACGGA 2 cut(s) 147, 287
TspRI CASTG 1 cut(s) 1260
Van91I CCANNNNNTGG 1 cut(s) 113
Vha464I CTTAAG 1 cut(s) 736
VpaK11BI GGWCC 1 cut(s) 847
XapI RAATTY 1 cut(s) 365
XmiI GTMKAC 1 cut(s) 867
XmnI GAANNNNTTC 1 cut(s) 785
XspI CTAG 3 cut(s) 231, 759, 890
ZrmI AGTACT 2 cut(s) 763, 888
Zsp2I ATGCAT 1 cut(s) 1049
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.