Rw6G023580

Membrane protein of ER body-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0002486
Physical Location & Seq
Reverse (-)
46181322 .. 46184401
3080 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G023580.1

Sequence Viewer

Length: 1305 bp
ATGGAAGCACCATTAGAAAGGCTGCCTCGCCAAAACAGCAACACAGACATTACCACCAATAATGGTGGGAATGTTGTCTATTCCTTAGAAGAAGGGAAGAGAAGCCCAAGAAATGGTACTCACATGGTCTCTGATAAAAGAGCAGGTACTGAATTGCTATACGGAGCTACATCCAATAGTGGACATGGAATAGTGACTGTTGGAGTAGAGAGCGTACGTTTTCAAAAGAGTGTTGAGACCAAGCACCAAGACGAGGATACTAGGAAGTTGAAGGATCTTGGCTCTTCATCCTCCGAGCAGACGGAGCAGTCCCCTGAGGAAAATGGAGTGGCTAAGCATCGAAAAATTAAGTTGAGAGATCTTGAAGGGGTGTTTGAGAAGACGGCTGCGCATGAATTCTACTGTCCTAATTGCAATGCTTGCATCACCGAGGTTATAGTCCGCGATAGAGAATCAAAACCTCCTACTCCACCACTTATTGAGCCAGATGAACCAAAACCTCCTACTCCACCACTTATTGAGCCAGATGAACCCGTCAGATGCACTTCGTGCTTTGGTCTCCTATTTTGTCTAGGCACTTGGATCTCTCGTCGACCAGGGCAAGAACCTGCAGAACCTACAGATGGCACAACAGTTATTACTGTGATCACACCGCCGGAACAAACGCCGGCAGGGTCTGAATTACCACGAGATGCTAATAAGATGTTAGAAATCCTCAAAAGCATCGTTTACGGTGGTTTAAATGAAGCAATTGCAAGCTTAAGCATTGTGGCATCTGCAGCTAGTACTGATACTGCCACACTGAACATTTTCGTTTTGGCACTGGCAAACTTGCTGGGTGGTCTTTTCGTCATCGGACACAATCTCTGGGACCTGAAAGCTGATCAAGTTGACAGTAAATACAAAGAAGTACTAGGCAACAAAGATAATTTCTTTTTTCATGTCCCGGTTGCCATACTCTCGTTCCTCATTTTCGGGTTACTACCTCCTTTGGTTTATGGCTTCTCGTTCTCTCAGAGCGACGACAGCGACCTAAAGATTGCAGCTGTTGCAGCATCTTCTATTTTATGCATCATACTTCTCGCAATTGGAAAGGCTTACATCCAGACGCCACCAAAGTACATCAAAACTGTGCTGTACTATATCGTCATAGGGGTTGGCGCCGCCGGTGTTTCTTACTTGGTAGGTGACTTAATTGACAAACTTGTAGAGAAGCTTGGCTGGTTCAACTCAACTGTAGCTGGCTCTCTGCCTCTCCCTCATCTGACTATGAGTACAGTGAAACCTGCATGGGAATCCTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VIT1

Protein Analysis

434

Amino Acids

47.15

Weight (kDa)

5.34

Isoelectric Point (pI)

43.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VIT1 PF01988 319 - 396 1e-06 VIT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 307
Acc16I TGCGCA 1 cut(s) 390
Acc36I ACCTGC 3 cut(s) 134, 616, 1294
AccB1I GGYRCC 1 cut(s) 1160
AccB7I CCANNNNNTGG 1 cut(s) 113
AccI GTMKAC 1 cut(s) 592
AccII CGCG 1 cut(s) 444
AciI CCGC 3 cut(s) 442, 653, 1164
AclWI GGATC 2 cut(s) 282, 590
AcsI RAATTY 1 cut(s) 395
AcyI GRCGYC 2 cut(s) 1109, 1161
AdeI CACNNNGTG 1 cut(s) 549
AfaI GTAC 8 cut(s) 118, 148, 216, 787, 912, 1121, 1139, 1276
AfiI CCNNNNNNNGG 3 cut(s) 113, 253, 623
AflII CTTAAG 1 cut(s) 760
AgsI TTSAA 4 cut(s) 224, 271, 365, 1228
AjnI CCWGG 2 cut(s) 595, 1298
AluBI AGCT 7 cut(s) 167, 759, 782, 881, 1046, 1216, 1241
AluI AGCT 7 cut(s) 167, 759, 782, 881, 1046, 1216, 1241
Alw26I GTCTC 3 cut(s) 133, 230, 563
AlwI GGATC 2 cut(s) 282, 590
AlwNI CAGNNNCTG 2 cut(s) 149, 677
ApeKI GCWGC 5 cut(s) 22, 386, 779, 1043, 1052
ApoI RAATTY 1 cut(s) 395
Asp700I GAANNNNTTC 1 cut(s) 809
AspLEI GCGC 2 cut(s) 391, 1163
AspS9I GGNCC 1 cut(s) 871
AsuC2I CCSGG 1 cut(s) 947
AsuHPI GGTGA 2 cut(s) 418, 1199
AvaII GGWCC 1 cut(s) 871
AxyI CCTNAGG 1 cut(s) 315
BanI GGYRCC 1 cut(s) 1160
BauI CACGAG 1 cut(s) 687
BbsI GAAGAC 1 cut(s) 386
BbvI GCAGC 5 cut(s) 9, 373, 791, 1055, 1064
BccI CCATC 1 cut(s) 617
BceAI ACGGC 1 cut(s) 399
BciT130I CCWGG 2 cut(s) 597, 1300
BciVI GTATCC 1 cut(s) 250
BclI TGATCA 2 cut(s) 645, 883
BcnI CCSGG 1 cut(s) 947
BcoDI GTCTC 3 cut(s) 133, 230, 563
BfaI CTAG 4 cut(s) 261, 572, 783, 914
BfmI CTRYAG 4 cut(s) 609, 618, 777, 1236
BfoI RGCGCY 1 cut(s) 1164
BfrI CTTAAG 1 cut(s) 760
BfuAI ACCTGC 3 cut(s) 134, 616, 1294
BfuI GTATCC 1 cut(s) 250
BglII AGATCT 1 cut(s) 358
BisI GCNGC 6 cut(s) 23, 387, 780, 1044, 1053, 1164
BlpI GCTNAGC 1 cut(s) 333
BlsI GCNGC 6 cut(s) 24, 388, 781, 1045, 1054, 1165
BmcAI AGTACT 2 cut(s) 787, 912
Bme1390I CCNGG 3 cut(s) 597, 947, 1300
Bme18I GGWCC 1 cut(s) 871
BmgT120I GGNCC 1 cut(s) 871
BmiI GGNNCC 2 cut(s) 872, 1162
BmrFI CCNGG 3 cut(s) 597, 947, 1300
BmsI GCATC 8 cut(s) 346, 432, 530, 682, 732, 782, 1064, 1080
BpiI GAAGAC 1 cut(s) 386
Bpu1102I GCTNAGC 1 cut(s) 333
BpuMI CCSGG 1 cut(s) 947
BsaHI GRCGYC 2 cut(s) 1109, 1161
BsaI GGTCTC 3 cut(s) 133, 230, 563
BsaJI CCNNGG 2 cut(s) 429, 596
Bsc4I CCNNNNNNNGG 3 cut(s) 113, 253, 623
Bse118I RCCGGY 2 cut(s) 667, 1166
Bse1I ACTGG 1 cut(s) 828
Bse21I CCTNAGG 1 cut(s) 315
Bse3DI GCAATG 1 cut(s) 421
BseBI CCWGG 2 cut(s) 597, 1300
BseDI CCNNGG 2 cut(s) 429, 596
BseGI GGATG 3 cut(s) 170, 287, 1101
BseLI CCNNNNNNNGG 3 cut(s) 113, 253, 623
BseMI GCAATG 1 cut(s) 421
BseMII CTCAG 2 cut(s) 306, 1028
BseNI ACTGG 1 cut(s) 828
BseXI GCAGC 5 cut(s) 9, 373, 791, 1055, 1064
BseYI CCCAGC 1 cut(s) 835
Bsh1236I CGCG 1 cut(s) 444
BshNI GGYRCC 1 cut(s) 1160
BsiSI CCGG 4 cut(s) 656, 668, 947, 1167
BsiWI CGTACG 1 cut(s) 214
BslFI GGGAC 3 cut(s) 295, 884, 929
BslI CCNNNNNNNGG 3 cut(s) 113, 253, 623
BsmAI GTCTC 3 cut(s) 133, 230, 563
BsmFI GGGAC 3 cut(s) 295, 884, 929
Bso31I GGTCTC 3 cut(s) 133, 230, 563
Bsp143I GATC 5 cut(s) 274, 358, 582, 645, 883
Bsp1720I GCTNAGC 1 cut(s) 333
BspACI CCGC 3 cut(s) 442, 653, 1164
BspCNI CTCAG 2 cut(s) 307, 1027
BspFNI CGCG 1 cut(s) 444
BspLI GGNNCC 2 cut(s) 872, 1162
BspMAI CTGCAG 2 cut(s) 613, 781
BspMI ACCTGC 3 cut(s) 134, 616, 1294
BspPI GGATC 2 cut(s) 282, 590
BspQI GCTCTTC 1 cut(s) 289
BspT107I GGYRCC 1 cut(s) 1160
BspTI CTTAAG 1 cut(s) 760
BspTNI GGTCTC 3 cut(s) 133, 230, 563
BsrDI GCAATG 1 cut(s) 421
BsrFI RCCGGY 2 cut(s) 667, 1166
BsrI ACTGG 1 cut(s) 828
BssAI RCCGGY 2 cut(s) 667, 1166
BssECI CCNNGG 2 cut(s) 429, 596
BssMI GATC 5 cut(s) 274, 358, 582, 645, 883
BssNI GRCGYC 2 cut(s) 1109, 1161
BssSI CACGAG 1 cut(s) 687
Bst2BI CACGAG 1 cut(s) 687
Bst2UI CCWGG 2 cut(s) 597, 1300
Bst4CI ACNGT 9 cut(s) 199, 404, 634, 643, 734, 896, 1132, 1237, 1279
Bst6I CTCTTC 2 cut(s) 92, 289
BstACI GRCGYC 2 cut(s) 1109, 1161
BstAFI CTTAAG 1 cut(s) 760
BstAPI GCANNNNNTGC 3 cut(s) 420, 549, 1049
BstC8I GCNNGC 4 cut(s) 421, 669, 757, 1243
BstDEI CTNAG 4 cut(s) 85, 315, 333, 1014
BstF5I GGATG 3 cut(s) 170, 287, 1101
BstFNI CGCG 1 cut(s) 444
BstH2I RGCGCY 1 cut(s) 1164
BstHHI GCGC 2 cut(s) 391, 1163
BstKTI GATC 5 cut(s) 277, 361, 585, 648, 886
BstMAI GTCTC 3 cut(s) 133, 230, 563
BstMBI GATC 5 cut(s) 274, 358, 582, 645, 883
BstMWI GCNNNNNNNGC 8 cut(s) 36, 304, 420, 549, 779, 1026, 1049, 1052
BstNI CCWGG 2 cut(s) 597, 1300
BstSCI CCNGG 3 cut(s) 595, 945, 1298
BstSFI CTRYAG 4 cut(s) 609, 618, 777, 1236
BstUI CGCG 1 cut(s) 444
BstV1I GCAGC 5 cut(s) 9, 373, 791, 1055, 1064
BstV2I GAAGAC 1 cut(s) 386
BstX2I RGATCY 3 cut(s) 274, 358, 582
BstYI RGATCY 3 cut(s) 274, 358, 582
Bsu36I CCTNAGG 1 cut(s) 315
BsuI GTATCC 1 cut(s) 250
BtsCI GGATG 3 cut(s) 170, 287, 1101
BtsIMutI CAGTG 3 cut(s) 800, 821, 1284
BveI ACCTGC 3 cut(s) 134, 616, 1294
Cac8I GCNNGC 4 cut(s) 421, 669, 757, 1243
CaiI CAGNNNCTG 2 cut(s) 149, 677
CfoI GCGC 2 cut(s) 391, 1163
Cfr10I RCCGGY 2 cut(s) 667, 1166
Cfr13I GGNCC 1 cut(s) 871
CseI GACGC 1 cut(s) 1117
Csp6I GTAC 8 cut(s) 117, 147, 215, 786, 911, 1120, 1138, 1275
CspCI CAANNNNNGTGG 2 cut(s) 46, 81
CviAII CATG 5 cut(s) 124, 185, 392, 941, 1290
CviQI GTAC 8 cut(s) 117, 147, 215, 786, 911, 1120, 1138, 1275
DdeI CTNAG 4 cut(s) 85, 315, 333, 1014
DinI GGCGCC 1 cut(s) 1162
DpnI GATC 5 cut(s) 276, 360, 584, 647, 885
DpnII GATC 5 cut(s) 274, 358, 582, 645, 883
DraI TTTAAA 1 cut(s) 741
DraIII CACNNNGTG 1 cut(s) 549
DrdI GACNNNNNNGTC 1 cut(s) 307
DseDI GACNNNNNNGTC 1 cut(s) 307
Eam1104I CTCTTC 2 cut(s) 92, 289
EarI CTCTTC 2 cut(s) 92, 289
Eco31I GGTCTC 3 cut(s) 133, 230, 563
Eco47I GGWCC 1 cut(s) 871
Eco81I CCTNAGG 1 cut(s) 315
EcoO109I RGGNCCY 1 cut(s) 871
EcoRI GAATTC 1 cut(s) 395
EcoRII CCWGG 2 cut(s) 595, 1298
EcoT22I ATGCAT 1 cut(s) 1073
EgeI GGCGCC 1 cut(s) 1162
EheI GGCGCC 1 cut(s) 1162
FaeI CATG 5 cut(s) 127, 188, 395, 944, 1293
FaqI GGGAC 3 cut(s) 295, 884, 929
FatI CATG 5 cut(s) 123, 184, 391, 940, 1289
FbaI TGATCA 2 cut(s) 645, 883
FblI GTMKAC 1 cut(s) 592
Fnu4HI GCNGC 6 cut(s) 23, 387, 780, 1044, 1053, 1164
FokI GGATG 3 cut(s) 157, 274, 1088
Fsp4HI GCNGC 6 cut(s) 23, 387, 780, 1044, 1053, 1164
FspBI CTAG 4 cut(s) 261, 572, 783, 914
FspI TGCGCA 1 cut(s) 390
GlaI GCGC 2 cut(s) 390, 1162
GluI GCNGC 6 cut(s) 23, 387, 780, 1044, 1053, 1164
GsaI CCCAGC 1 cut(s) 839
HaeII RGCGCY 1 cut(s) 1164
HapII CCGG 4 cut(s) 656, 668, 947, 1167
HgaI GACGC 1 cut(s) 1117
HhaI GCGC 2 cut(s) 391, 1163
Hin1I GRCGYC 2 cut(s) 1109, 1161
Hin1II CATG 5 cut(s) 127, 188, 395, 944, 1293
Hin6I GCGC 2 cut(s) 389, 1161
HinP1I GCGC 2 cut(s) 389, 1161
HincII GTYRAC 2 cut(s) 593, 892
HindII GTYRAC 2 cut(s) 593, 892
HindIII AAGCTT 2 cut(s) 757, 1214
HinfI GANTC 2 cut(s) 452, 1295
HpaII CCGG 4 cut(s) 656, 668, 947, 1167
HphI GGTGA 2 cut(s) 418, 1199
Hpy166II GTNNAC 4 cut(s) 182, 593, 730, 892
Hpy188I TCNGA 7 cut(s) 133, 295, 539, 679, 857, 1017, 1266
Hpy188III TCNNGA 2 cut(s) 362, 1105
Hpy8I GTNNAC 4 cut(s) 182, 593, 730, 892
Hpy99I CGWCG 2 cut(s) 594, 1025
HpyAV CCTTC 3 cut(s) 86, 265, 359
HpyCH4III ACNGT 9 cut(s) 199, 404, 634, 643, 734, 896, 1132, 1237, 1279
HpyCH4IV ACGT 1 cut(s) 217
HpyF10VI GCNNNNNNNGC 8 cut(s) 36, 304, 420, 549, 779, 1026, 1049, 1052
HpyF3I CTNAG 4 cut(s) 85, 315, 333, 1014
HpySE526I ACGT 1 cut(s) 217
Hsp92I GRCGYC 2 cut(s) 1109, 1161
Hsp92II CATG 5 cut(s) 127, 188, 395, 944, 1293
HspAI GCGC 2 cut(s) 389, 1161
KasI GGCGCC 1 cut(s) 1160
KroI GCCGGC 1 cut(s) 667
KroNI GCCGGC 1 cut(s) 669
Ksp22I TGATCA 2 cut(s) 645, 883
Kzo9I GATC 5 cut(s) 274, 358, 582, 645, 883
LguI GCTCTTC 1 cut(s) 289
LmnI GCTCC 2 cut(s) 164, 304
Lsp1109I GCAGC 5 cut(s) 9, 373, 791, 1055, 1064
LweI GCATC 8 cut(s) 346, 432, 530, 682, 732, 782, 1064, 1080
MaeI CTAG 4 cut(s) 261, 572, 783, 914
MaeII ACGT 1 cut(s) 217
MaeIII GTNAC 3 cut(s) 193, 978, 1187
MalI GATC 5 cut(s) 276, 360, 584, 647, 885
MboI GATC 5 cut(s) 274, 358, 582, 645, 883
MboII GAAGA 5 cut(s) 101, 109, 276, 391, 1050
MfeI CAATTG 2 cut(s) 750, 1086
MflI RGATCY 3 cut(s) 274, 358, 582
MluCI AATT 9 cut(s) 152, 345, 395, 409, 680, 750, 928, 1086, 1194
Mly113I GGCGCC 1 cut(s) 1161
MmeI TCCRAC 1 cut(s) 181
Mph1103I ATGCAT 1 cut(s) 1073
MroNI GCCGGC 1 cut(s) 667
MroXI GAANNNNTTC 1 cut(s) 809
MseI TTAA 4 cut(s) 348, 740, 761, 1193
MspA1I CMGCKG 1 cut(s) 1046
MspCI CTTAAG 1 cut(s) 760
MspI CCGG 4 cut(s) 656, 668, 947, 1167
MspR9I CCNGG 3 cut(s) 597, 947, 1300
MunI CAATTG 2 cut(s) 750, 1086
MvaI CCWGG 2 cut(s) 597, 1300
MvnI CGCG 1 cut(s) 444
MwoI GCNNNNNNNGC 8 cut(s) 36, 304, 420, 549, 779, 1026, 1049, 1052
NaeI GCCGGC 1 cut(s) 669
NarI GGCGCC 1 cut(s) 1161
NciI CCSGG 1 cut(s) 947
NdeII GATC 5 cut(s) 274, 358, 582, 645, 883
NgoMIV GCCGGC 1 cut(s) 667
NlaIII CATG 5 cut(s) 127, 188, 395, 944, 1293
NlaIV GGNNCC 2 cut(s) 872, 1162
NmuCI GTSAC 2 cut(s) 193, 1187
NsbI TGCGCA 1 cut(s) 390
NsiI ATGCAT 1 cut(s) 1073
PciSI GCTCTTC 1 cut(s) 289
PdiI GCCGGC 1 cut(s) 669
PdmI GAANNNNTTC 1 cut(s) 809
PfeI GAWTC 2 cut(s) 452, 1295
Pfl23II CGTACG 1 cut(s) 214
PflMI CCANNNNNTGG 1 cut(s) 113
PkrI GCNGC 6 cut(s) 24, 388, 781, 1045, 1054, 1165
PluTI GGCGCC 1 cut(s) 1164
PpuMI RGGWCCY 1 cut(s) 871
Psp5II RGGWCCY 1 cut(s) 871
Psp6I CCWGG 2 cut(s) 595, 1298
PspFI CCCAGC 1 cut(s) 835
PspGI CCWGG 2 cut(s) 595, 1298
PspLI CGTACG 1 cut(s) 214
PspN4I GGNNCC 2 cut(s) 872, 1162
PspPI GGNCC 1 cut(s) 871
PspPPI RGGWCCY 1 cut(s) 871
PstI CTGCAG 2 cut(s) 613, 781
PstNI CAGNNNCTG 2 cut(s) 149, 677
PsuI RGATCY 3 cut(s) 274, 358, 582
PvuII CAGCTG 1 cut(s) 1046
RsaI GTAC 8 cut(s) 118, 148, 216, 787, 912, 1121, 1139, 1276
RsaNI GTAC 8 cut(s) 117, 147, 215, 786, 911, 1120, 1138, 1275
SalI GTCGAC 1 cut(s) 591
SapI GCTCTTC 1 cut(s) 289
SaqAI TTAA 4 cut(s) 348, 740, 761, 1193
SatI GCNGC 6 cut(s) 23, 387, 780, 1044, 1053, 1164
Sau3AI GATC 5 cut(s) 274, 358, 582, 645, 883
Sau96I GGNCC 1 cut(s) 871
ScaI AGTACT 2 cut(s) 787, 912
ScrFI CCNGG 3 cut(s) 597, 947, 1300
SfaNI GCATC 8 cut(s) 346, 432, 530, 682, 732, 782, 1064, 1080
SfcI CTRYAG 4 cut(s) 609, 618, 777, 1236
SfoI GGCGCC 1 cut(s) 1162
SgrAI CRCCGGYG 1 cut(s) 1166
SinI GGWCC 1 cut(s) 871
SmlI CTYRAG 1 cut(s) 760
SmoI CTYRAG 1 cut(s) 760
Sse9I AATT 9 cut(s) 152, 345, 395, 409, 680, 750, 928, 1086, 1194
SsiI CCGC 3 cut(s) 442, 653, 1164
SspDI GGCGCC 1 cut(s) 1160
SspMI CTAG 4 cut(s) 261, 572, 783, 914
StyD4I CCNGG 3 cut(s) 595, 945, 1298
TaaI ACNGT 9 cut(s) 199, 404, 634, 643, 734, 896, 1132, 1237, 1279
TaiI ACGT 1 cut(s) 220
TaqI TCGA 2 cut(s) 340, 592
TasI AATT 9 cut(s) 152, 345, 395, 409, 680, 750, 928, 1086, 1194
TatI WGTACW 5 cut(s) 785, 910, 1119, 1137, 1274
TauI GCSGC 1 cut(s) 1166
TfiI GAWTC 2 cut(s) 452, 1295
Tru1I TTAA 4 cut(s) 348, 740, 761, 1193
Tru9I TTAA 4 cut(s) 348, 740, 761, 1193
TscAI CASTG 3 cut(s) 807, 828, 1284
TseFI GTSAC 2 cut(s) 193, 1187
TseI GCWGC 5 cut(s) 22, 386, 779, 1043, 1052
Tsp45I GTSAC 2 cut(s) 193, 1187
TspDTI ATGAA 6 cut(s) 276, 408, 504, 543, 759, 929
TspGWI ACGGA 2 cut(s) 177, 317
TspRI CASTG 3 cut(s) 807, 828, 1284
Van91I CCANNNNNTGG 1 cut(s) 113
Vha464I CTTAAG 1 cut(s) 760
VpaK11BI GGWCC 1 cut(s) 871
XapI RAATTY 1 cut(s) 395
XmiI GTMKAC 1 cut(s) 592
XmnI GAANNNNTTC 1 cut(s) 809
XspI CTAG 4 cut(s) 261, 572, 783, 914
ZrmI AGTACT 2 cut(s) 787, 912
Zsp2I ATGCAT 1 cut(s) 1073
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.