Rroxscaffold_7G00184880

Membrane protein of ER body-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0002486
Physical Location & Seq
Forward (+)
24181214 .. 24183507
2294 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00184880.1

Sequence Viewer

Length: 723 bp
ATGTTGTTGTCCGAAGACGGCCCTCAATCGCGCGCAGCTGCGGCCGGGCATCTGCCGAGTCATCTGCCGAGCTATCACACAACGAGGTCGCAGCTTGGAGATGGAGCCTCCGAGCCGCCGCCGATTTCGTTGGAGGAGCGGCTGCCGGCGAAGCCGGCTTCGAGTCGCGGTGACGGAAGTGGTCGAACGACCTTGATGGTAGCGGTGGCCATGGGTTCATCGACCGCTTGGGTCGCCGCTGCCTCGATATTGACCATGAAAGTTTTCGGTGGGATTCCAATTTTGGGACTTTGGGACCTGAAAGCTGATCAAGTAGACAGTAAATACAAAGAAGTACTAGGGAACAAAGATAATTTCTTTTTTCATGCCCCAGTTGCCATACTCTCGTTCCTCATTTTCGGGTTAGTACCTCCTTTGGTTTATGGCTTCTCGTTCCCTCAGAGCGACGACAGCGACCTAAAGATTGCAGCAGTTGCATCATCTTCTCTTTTATGCATCATACTTCTCGCAATTGGAAAGGCCTACATCCAGACTCCACCAAAGTACATCAAAACTGTGCTGTACTATGTCGTCATCTGGGTTGGGGCCGCCGGTGTTTCTTACTTAGTAGGTGACTTAATTGACAAACTTGTAGAGAAGCTTGGCTGGTTCAACTCAACTGTAGCTGGCTCTCTGCCTCTTCAGACTATGAGTAGAGTGAAGCCTGCATGGGAATCCTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VIT1

Protein Analysis

240

Amino Acids

25.67

Weight (kDa)

6.9

Isoelectric Point (pI)

44.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VIT1 PF01988 122 - 204 2.3e-06 VIT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 139
AccI GTMKAC 1 cut(s) 315
AccII CGCG 3 cut(s) 31, 33, 168
AciI CCGC 9 cut(s) 41, 116, 119, 139, 168, 203, 225, 237, 588
AcoI YGGCCR 2 cut(s) 42, 207
AcuI CTGAAG 1 cut(s) 665
AfaI GTAC 4 cut(s) 336, 408, 545, 563
AfiI CCNNNNNNNGG 1 cut(s) 284
AgsI TTSAA 1 cut(s) 652
AjnI CCWGG 1 cut(s) 716
AluBI AGCT 6 cut(s) 38, 72, 94, 305, 640, 665
AluI AGCT 6 cut(s) 38, 72, 94, 305, 640, 665
AoxI GGCC 5 cut(s) 19, 42, 207, 519, 585
ApeKI GCWGC 6 cut(s) 35, 38, 91, 142, 239, 467
Asp700I GAANNNNTTC 1 cut(s) 263
AspLEI GCGC 2 cut(s) 33, 35
AspS9I GGNCC 3 cut(s) 20, 295, 585
AsuC2I CCSGG 1 cut(s) 46
AsuHPI GGTGA 2 cut(s) 182, 623
AvaII GGWCC 1 cut(s) 295
BalI TGGCCA 1 cut(s) 209
BbsI GAAGAC 1 cut(s) 21
BbvI GCAGC 6 cut(s) 25, 47, 103, 129, 226, 479
BccI CCATC 2 cut(s) 95, 190
BceAI ACGGC 1 cut(s) 34
BcgI CGANNNNNNTGC 2 cut(s) 46, 80
BciT130I CCWGG 1 cut(s) 718
BclI TGATCA 1 cut(s) 307
BcnI CCSGG 1 cut(s) 46
BfaI CTAG 1 cut(s) 338
BfmI CTRYAG 1 cut(s) 660
BmcAI AGTACT 1 cut(s) 336
Bme1390I CCNGG 2 cut(s) 46, 718
Bme18I GGWCC 1 cut(s) 295
BmgT120I GGNCC 3 cut(s) 20, 295, 585
BmiI GGNNCC 3 cut(s) 106, 296, 586
BmrFI CCNGG 2 cut(s) 46, 718
BmrI ACTGGG 1 cut(s) 365
BmsI GCATC 3 cut(s) 58, 485, 504
BmuI ACTGGG 1 cut(s) 365
BpiI GAAGAC 1 cut(s) 21
BpuMI CCSGG 1 cut(s) 46
BsaJI CCNNGG 1 cut(s) 210
Bsc4I CCNNNNNNNGG 1 cut(s) 284
Bse118I RCCGGY 3 cut(s) 145, 154, 590
Bse1I ACTGG 1 cut(s) 371
BseBI CCWGG 1 cut(s) 718
BseDI CCNNGG 1 cut(s) 210
BseGI GGATG 1 cut(s) 525
BseLI CCNNNNNNNGG 1 cut(s) 284
BseMII CTCAG 1 cut(s) 452
BseNI ACTGG 1 cut(s) 371
BsePI GCGCGC 1 cut(s) 31
BseRI GAGGAG 1 cut(s) 149
BseX3I CGGCCG 1 cut(s) 42
BseXI GCAGC 6 cut(s) 25, 47, 103, 129, 226, 479
Bsh1236I CGCG 3 cut(s) 31, 33, 168
Bsh1285I CGRYCG 2 cut(s) 45, 225
BshFI GGCC 5 cut(s) 21, 44, 209, 521, 587
BsiEI CGRYCG 2 cut(s) 45, 225
BsiSI CCGG 4 cut(s) 45, 146, 155, 591
BslFI GGGAC 2 cut(s) 300, 308
BslI CCNNNNNNNGG 1 cut(s) 284
BsmFI GGGAC 2 cut(s) 300, 308
BsnI GGCC 5 cut(s) 21, 44, 209, 521, 587
Bsp143I GATC 1 cut(s) 307
Bsp19I CCATGG 1 cut(s) 210
BspACI CCGC 9 cut(s) 41, 116, 119, 139, 168, 203, 225, 237, 588
BspANI GGCC 5 cut(s) 21, 44, 209, 521, 587
BspCNI CTCAG 1 cut(s) 451
BspFNI CGCG 3 cut(s) 31, 33, 168
BspLI GGNNCC 3 cut(s) 106, 296, 586
BsrBI CCGCTC 1 cut(s) 139
BsrFI RCCGGY 3 cut(s) 145, 154, 590
BsrI ACTGG 1 cut(s) 371
BssAI RCCGGY 3 cut(s) 145, 154, 590
BssECI CCNNGG 1 cut(s) 210
BssHII GCGCGC 1 cut(s) 31
BssMI GATC 1 cut(s) 307
BssT1I CCWWGG 1 cut(s) 210
Bst2UI CCWGG 1 cut(s) 718
Bst4CI ACNGT 3 cut(s) 320, 556, 661
Bst6I CTCTTC 1 cut(s) 684
BstAPI GCANNNNNTGC 1 cut(s) 473
BstC8I GCNNGC 5 cut(s) 33, 147, 156, 667, 705
BstDEI CTNAG 2 cut(s) 438, 604
BstDSI CCRYGG 1 cut(s) 210
BstF5I GGATG 1 cut(s) 525
BstFNI CGCG 3 cut(s) 31, 33, 168
BstHHI GCGC 2 cut(s) 33, 35
BstKTI GATC 1 cut(s) 310
BstMBI GATC 1 cut(s) 307
BstMCI CGRYCG 2 cut(s) 45, 225
BstMWI GCNNNNNNNGC 7 cut(s) 41, 151, 155, 233, 374, 450, 473
BstNI CCWGG 1 cut(s) 718
BstSCI CCNGG 2 cut(s) 44, 716
BstSFI CTRYAG 1 cut(s) 660
BstUI CGCG 3 cut(s) 31, 33, 168
BstV1I GCAGC 6 cut(s) 25, 47, 103, 129, 226, 479
BstV2I GAAGAC 1 cut(s) 21
BstZI CGGCCG 1 cut(s) 42
BsuRI GGCC 5 cut(s) 21, 44, 209, 521, 587
BtgI CCRYGG 1 cut(s) 210
BtsCI GGATG 1 cut(s) 525
Cac8I GCNNGC 5 cut(s) 33, 147, 156, 667, 705
CfoI GCGC 2 cut(s) 33, 35
Cfr10I RCCGGY 3 cut(s) 145, 154, 590
Cfr13I GGNCC 3 cut(s) 20, 295, 585
Csp6I GTAC 4 cut(s) 335, 407, 544, 562
CviAII CATG 4 cut(s) 211, 256, 365, 708
CviQI GTAC 4 cut(s) 335, 407, 544, 562
DdeI CTNAG 2 cut(s) 438, 604
DpnI GATC 1 cut(s) 309
DpnII GATC 1 cut(s) 307
EaeI YGGCCR 2 cut(s) 42, 207
EagI CGGCCG 1 cut(s) 42
Eam1104I CTCTTC 1 cut(s) 684
EarI CTCTTC 1 cut(s) 684
EclXI CGGCCG 1 cut(s) 42
Eco130I CCWWGG 1 cut(s) 210
Eco147I AGGCCT 1 cut(s) 521
Eco47I GGWCC 1 cut(s) 295
Eco52I CGGCCG 1 cut(s) 42
Eco57I CTGAAG 1 cut(s) 665
EcoO109I RGGNCCY 1 cut(s) 295
EcoRII CCWGG 1 cut(s) 716
EcoT14I CCWWGG 1 cut(s) 210
EcoT22I ATGCAT 1 cut(s) 497
ErhI CCWWGG 1 cut(s) 210
FaeI CATG 4 cut(s) 214, 259, 368, 711
FaqI GGGAC 2 cut(s) 300, 308
FatI CATG 4 cut(s) 210, 255, 364, 707
FbaI TGATCA 1 cut(s) 307
FblI GTMKAC 1 cut(s) 315
FokI GGATG 1 cut(s) 512
FspBI CTAG 1 cut(s) 338
GlaI GCGC 2 cut(s) 32, 34
HaeIII GGCC 5 cut(s) 21, 44, 209, 521, 587
HapII CCGG 4 cut(s) 45, 146, 155, 591
HhaI GCGC 2 cut(s) 33, 35
Hin1II CATG 4 cut(s) 214, 259, 368, 711
Hin6I GCGC 2 cut(s) 31, 33
HinP1I GCGC 2 cut(s) 31, 33
HindIII AAGCTT 1 cut(s) 638
HinfI GANTC 5 cut(s) 58, 163, 274, 532, 713
HpaII CCGG 4 cut(s) 45, 146, 155, 591
HphI GGTGA 2 cut(s) 182, 623
Hpy166II GTNNAC 1 cut(s) 316
Hpy188I TCNGA 4 cut(s) 13, 112, 441, 684
Hpy188III TCNNGA 1 cut(s) 529
Hpy8I GTNNAC 1 cut(s) 316
Hpy99I CGWCG 1 cut(s) 449
HpyCH4III ACNGT 3 cut(s) 320, 556, 661
HpyCH4V TGCA 4 cut(s) 467, 476, 495, 707
HpyF10VI GCNNNNNNNGC 7 cut(s) 41, 151, 155, 233, 374, 450, 473
HpyF3I CTNAG 2 cut(s) 438, 604
Hsp92II CATG 4 cut(s) 214, 259, 368, 711
HspAI GCGC 2 cut(s) 31, 33
KroI GCCGGC 2 cut(s) 145, 154
KroNI GCCGGC 2 cut(s) 147, 156
Ksp22I TGATCA 1 cut(s) 307
Kzo9I GATC 1 cut(s) 307
LmnI GCTCC 2 cut(s) 104, 136
Lsp1109I GCAGC 6 cut(s) 25, 47, 103, 129, 226, 479
LweI GCATC 3 cut(s) 58, 485, 504
MaeI CTAG 1 cut(s) 338
MaeIII GTNAC 2 cut(s) 170, 611
MalI GATC 1 cut(s) 309
MbiI CCGCTC 1 cut(s) 139
MboI GATC 1 cut(s) 307
MboII GAAGA 3 cut(s) 26, 474, 671
MfeI CAATTG 1 cut(s) 510
MlsI TGGCCA 1 cut(s) 209
MluCI AATT 4 cut(s) 279, 352, 510, 618
MluNI TGGCCA 1 cut(s) 209
MlyI GAGTC 3 cut(s) 67, 172, 526
MmeI TCCRAC 1 cut(s) 111
MnlI CCTC 9 cut(s) 33, 78, 118, 127, 253, 401, 420, 447, 687
Mox20I TGGCCA 1 cut(s) 209
Mph1103I ATGCAT 1 cut(s) 497
MroNI GCCGGC 2 cut(s) 145, 154
MroXI GAANNNNTTC 1 cut(s) 263
MscI TGGCCA 1 cut(s) 209
MseI TTAA 1 cut(s) 617
Msp20I TGGCCA 1 cut(s) 209
MspA1I CMGCKG 2 cut(s) 38, 239
MspI CCGG 4 cut(s) 45, 146, 155, 591
MspR9I CCNGG 2 cut(s) 46, 718
MunI CAATTG 1 cut(s) 510
MvaI CCWGG 1 cut(s) 718
MvnI CGCG 3 cut(s) 31, 33, 168
MwoI GCNNNNNNNGC 7 cut(s) 41, 151, 155, 233, 374, 450, 473
NaeI GCCGGC 2 cut(s) 147, 156
NciI CCSGG 1 cut(s) 46
NcoI CCATGG 1 cut(s) 210
NdeII GATC 1 cut(s) 307
NgoMIV GCCGGC 2 cut(s) 145, 154
NlaIII CATG 4 cut(s) 214, 259, 368, 711
NlaIV GGNNCC 3 cut(s) 106, 296, 586
NmeAIII GCCGAG 2 cut(s) 81, 93
NmuCI GTSAC 2 cut(s) 170, 611
NsiI ATGCAT 1 cut(s) 497
PauI GCGCGC 1 cut(s) 31
PceI AGGCCT 1 cut(s) 521
PdiI GCCGGC 2 cut(s) 147, 156
PdmI GAANNNNTTC 1 cut(s) 263
PfeI GAWTC 2 cut(s) 274, 713
PleI GAGTC 3 cut(s) 66, 171, 526
PpsI GAGTC 3 cut(s) 66, 171, 526
PpuMI RGGWCCY 1 cut(s) 295
Psp5II RGGWCCY 1 cut(s) 295
Psp6I CCWGG 1 cut(s) 716
PspGI CCWGG 1 cut(s) 716
PspN4I GGNNCC 3 cut(s) 106, 296, 586
PspPI GGNCC 3 cut(s) 20, 295, 585
PspPPI RGGWCCY 1 cut(s) 295
PteI GCGCGC 1 cut(s) 31
PvuII CAGCTG 1 cut(s) 38
RsaI GTAC 4 cut(s) 336, 408, 545, 563
RsaNI GTAC 4 cut(s) 335, 407, 544, 562
SaqAI TTAA 1 cut(s) 617
Sau3AI GATC 1 cut(s) 307
Sau96I GGNCC 3 cut(s) 20, 295, 585
ScaI AGTACT 1 cut(s) 336
SchI GAGTC 3 cut(s) 67, 172, 526
ScrFI CCNGG 2 cut(s) 46, 718
SfaNI GCATC 3 cut(s) 58, 485, 504
SfcI CTRYAG 1 cut(s) 660
SgrAI CRCCGGYG 1 cut(s) 590
SinI GGWCC 1 cut(s) 295
Sse9I AATT 4 cut(s) 279, 352, 510, 618
SseBI AGGCCT 1 cut(s) 521
SsiI CCGC 9 cut(s) 41, 116, 119, 139, 168, 203, 225, 237, 588
SspMI CTAG 1 cut(s) 338
StuI AGGCCT 1 cut(s) 521
StyD4I CCNGG 2 cut(s) 44, 716
StyI CCWWGG 1 cut(s) 210
TaaI ACNGT 3 cut(s) 320, 556, 661
TaqI TCGA 4 cut(s) 161, 184, 221, 245
TasI AATT 4 cut(s) 279, 352, 510, 618
TatI WGTACW 3 cut(s) 334, 543, 561
TauI GCSGC 6 cut(s) 44, 118, 121, 142, 239, 590
TfiI GAWTC 2 cut(s) 274, 713
Tru1I TTAA 1 cut(s) 617
Tru9I TTAA 1 cut(s) 617
TseFI GTSAC 2 cut(s) 170, 611
TseI GCWGC 6 cut(s) 35, 38, 91, 142, 239, 467
Tsp45I GTSAC 2 cut(s) 170, 611
TspDTI ATGAA 3 cut(s) 207, 272, 353
TspGWI ACGGA 1 cut(s) 189
VpaK11BI GGWCC 1 cut(s) 295
XmiI GTMKAC 1 cut(s) 315
XmnI GAANNNNTTC 1 cut(s) 263
XspI CTAG 1 cut(s) 338
ZrmI AGTACT 1 cut(s) 336
Zsp2I ATGCAT 1 cut(s) 497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.