RLG00000000810

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0007553
Physical Location & Seq
Reverse (-)
5793729 .. 5794323
595 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000000810

Sequence Viewer

Length: 510 bp
ATGGAAGACACAAGTCGAGAGCATTCTAGGGTTTTGTTAAATAAGTATCTCTTGGAGCTTGTTTGGCGGCACCCCGACGACAACGGCTGCAGCTGGATGGGCTATGCTGTTGTCAGTTCAAAGACCTTGGTGGGAGATGCGGCGCCATTTTTTTTTTCCTCAAGTGAAATTGATGGCGGTTTCAAGGAGTTCATGGTGGTGGTCTGGTGTTCTTTGGCAGATTGCTGGGCCATGGTTGAAGGTTCATGTGAGAAGGGGATACCAGAATTAGCAATGGTGGGCATGGTGGTGTACAGCGCCATGGCCTACAATTGTTGGCTTGTTCTGGTACTTTTAGTCTTTGCGTGGTGGTCTGCTATTGGAGGGGATATGCGAAATGGCTTGGTACAACAGCAGCGGCCTTGCCGAAATGTGGTTACCGGCACCAACCTCATTGTGTATCTTTCTATTTGGACGGAGTCGGAGTTTTGGTTGTGGAAGATGAAGATTGACGACGTCATCTCTCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

170

Amino Acids

19.2

Weight (kDa)

4.72

Isoelectric Point (pI)

32.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 498
AccB1I GGYRCC 3 cut(s) 69, 142, 422
AciI CCGC 4 cut(s) 67, 140, 177, 397
AcyI GRCGYC 2 cut(s) 143, 495
AfaI GTAC 3 cut(s) 293, 330, 387
AfiI CCNNNNNNNGG 1 cut(s) 412
AgsI TTSAA 3 cut(s) 120, 184, 239
AluBI AGCT 2 cut(s) 58, 93
AluI AGCT 2 cut(s) 58, 93
AoxI GGCC 3 cut(s) 228, 303, 398
ApeKI GCWGC 3 cut(s) 87, 90, 394
AspLEI GCGC 2 cut(s) 145, 299
AspS9I GGNCC 1 cut(s) 228
BanI GGYRCC 3 cut(s) 69, 142, 422
BbsI GAAGAC 1 cut(s) 12
BbvI GCAGC 3 cut(s) 74, 102, 406
BccI CCATC 2 cut(s) 91, 167
BceAI ACGGC 1 cut(s) 100
BciVI GTATCC 1 cut(s) 252
BfaI CTAG 2 cut(s) 27, 508
BfmI CTRYAG 1 cut(s) 88
BfoI RGCGCY 2 cut(s) 146, 300
BfuI GTATCC 1 cut(s) 252
BisI GCNGC 6 cut(s) 68, 88, 91, 141, 395, 398
BlsI GCNGC 6 cut(s) 69, 89, 92, 142, 396, 399
BmgT120I GGNCC 1 cut(s) 228
BmiI GGNNCC 3 cut(s) 71, 144, 424
BmsI GCATC 1 cut(s) 127
BoxI GACNNNNGTC 1 cut(s) 12
BpiI GAAGAC 1 cut(s) 12
BpuEI CTTGAG 1 cut(s) 145
BsaHI GRCGYC 2 cut(s) 143, 495
BsaJI CCNNGG 3 cut(s) 126, 231, 300
BsaXI ACNNNNNCTCC 4 cut(s) 449, 455, 479, 485
Bsc4I CCNNNNNNNGG 1 cut(s) 412
Bse118I RCCGGY 1 cut(s) 419
Bse3DI GCAATG 1 cut(s) 279
BseDI CCNNGG 3 cut(s) 126, 231, 300
BseGI GGATG 1 cut(s) 102
BseLI CCNNNNNNNGG 1 cut(s) 412
BseMI GCAATG 1 cut(s) 279
BseXI GCAGC 3 cut(s) 74, 102, 406
BseYI CCCAGC 1 cut(s) 225
BshFI GGCC 3 cut(s) 230, 305, 400
BshNI GGYRCC 3 cut(s) 69, 142, 422
BsiSI CCGG 1 cut(s) 420
BslI CCNNNNNNNGG 1 cut(s) 412
BsmI GAATGC 1 cut(s) 22
BsnI GGCC 3 cut(s) 230, 305, 400
Bsp1407I TGTACA 1 cut(s) 291
Bsp19I CCATGG 2 cut(s) 231, 300
BspACI CCGC 4 cut(s) 67, 140, 177, 397
BspANI GGCC 3 cut(s) 230, 305, 400
BspLI GGNNCC 3 cut(s) 71, 144, 424
BspMAI CTGCAG 1 cut(s) 92
BspT107I GGYRCC 3 cut(s) 69, 142, 422
BsrDI GCAATG 1 cut(s) 279
BsrFI RCCGGY 1 cut(s) 419
BsrGI TGTACA 1 cut(s) 291
BssAI RCCGGY 1 cut(s) 419
BssECI CCNNGG 3 cut(s) 126, 231, 300
BssNI GRCGYC 2 cut(s) 143, 495
BssT1I CCWWGG 3 cut(s) 126, 231, 300
BstACI GRCGYC 2 cut(s) 143, 495
BstAUI TGTACA 1 cut(s) 291
BstDSI CCRYGG 2 cut(s) 231, 300
BstEII GGTNACC 1 cut(s) 415
BstF5I GGATG 1 cut(s) 102
BstH2I RGCGCY 2 cut(s) 146, 300
BstHHI GCGC 2 cut(s) 145, 299
BstMWI GCNNNNNNNGC 2 cut(s) 64, 99
BstPAI GACNNNNGTC 1 cut(s) 12
BstPI GGTNACC 1 cut(s) 415
BstSFI CTRYAG 1 cut(s) 88
BstV1I GCAGC 3 cut(s) 74, 102, 406
BstV2I GAAGAC 1 cut(s) 12
BsuI GTATCC 1 cut(s) 252
BsuRI GGCC 3 cut(s) 230, 305, 400
BtgI CCRYGG 2 cut(s) 231, 300
BtsCI GGATG 1 cut(s) 102
CfoI GCGC 2 cut(s) 145, 299
Cfr10I RCCGGY 1 cut(s) 419
Cfr13I GGNCC 1 cut(s) 228
Csp6I GTAC 3 cut(s) 292, 329, 386
CviAII CATG 5 cut(s) 193, 232, 246, 283, 301
CviJI RGCY 9 cut(s) 58, 87, 93, 102, 230, 305, 319, 381, 400
CviKI_1 RGCY 9 cut(s) 58, 87, 93, 102, 230, 305, 319, 381, 400
CviQI GTAC 3 cut(s) 292, 329, 386
DinI GGCGCC 1 cut(s) 144
Eco130I CCWWGG 3 cut(s) 126, 231, 300
Eco91I GGTNACC 1 cut(s) 415
EcoO65I GGTNACC 1 cut(s) 415
EcoT14I CCWWGG 3 cut(s) 126, 231, 300
EgeI GGCGCC 1 cut(s) 144
EheI GGCGCC 1 cut(s) 144
ErhI CCWWGG 3 cut(s) 126, 231, 300
FaeI CATG 5 cut(s) 196, 235, 249, 286, 304
FaiI YATR 7 cut(s) 105, 194, 233, 247, 284, 302, 371
FalI AAGNNNNNCTT 2 cut(s) 35, 67
FatI CATG 5 cut(s) 192, 231, 245, 282, 300
Fnu4HI GCNGC 6 cut(s) 68, 88, 91, 141, 395, 398
FokI GGATG 1 cut(s) 109
Fsp4HI GCNGC 6 cut(s) 68, 88, 91, 141, 395, 398
FspBI CTAG 2 cut(s) 27, 508
GlaI GCGC 2 cut(s) 144, 298
GluI GCNGC 6 cut(s) 68, 88, 91, 141, 395, 398
GsaI CCCAGC 1 cut(s) 229
HaeII RGCGCY 2 cut(s) 146, 300
HaeIII GGCC 3 cut(s) 230, 305, 400
HapII CCGG 1 cut(s) 420
HhaI GCGC 2 cut(s) 145, 299
Hin1I GRCGYC 2 cut(s) 143, 495
Hin1II CATG 5 cut(s) 196, 235, 249, 286, 304
Hin6I GCGC 2 cut(s) 143, 297
HinP1I GCGC 2 cut(s) 143, 297
HinfI GANTC 1 cut(s) 458
HpaII CCGG 1 cut(s) 420
Hpy166II GTNNAC 1 cut(s) 292
Hpy188I TCNGA 1 cut(s) 463
Hpy188III TCNNGA 1 cut(s) 17
Hpy8I GTNNAC 1 cut(s) 292
Hpy99I CGWCG 2 cut(s) 80, 497
HpyAV CCTTC 2 cut(s) 233, 247
HpyCH4IV ACGT 1 cut(s) 495
HpyCH4V TGCA 1 cut(s) 90
HpyF10VI GCNNNNNNNGC 2 cut(s) 64, 99
HpySE526I ACGT 1 cut(s) 495
Hsp92I GRCGYC 2 cut(s) 143, 495
Hsp92II CATG 5 cut(s) 196, 235, 249, 286, 304
HspAI GCGC 2 cut(s) 143, 297
KasI GGCGCC 1 cut(s) 142
LmnI GCTCC 1 cut(s) 55
LpnPI CCDG 6 cut(s) 79, 190, 211, 276, 311, 433
Lsp1109I GCAGC 3 cut(s) 74, 102, 406
LweI GCATC 1 cut(s) 127
MaeI CTAG 2 cut(s) 27, 508
MaeII ACGT 1 cut(s) 495
MaeIII GTNAC 1 cut(s) 415
MboII GAAGA 3 cut(s) 17, 490, 496
MfeI CAATTG 1 cut(s) 310
MluCI AATT 3 cut(s) 168, 266, 310
Mly113I GGCGCC 1 cut(s) 143
MlyI GAGTC 1 cut(s) 467
MmeI TCCRAC 1 cut(s) 441
MnlI CCTC 3 cut(s) 169, 356, 440
MseI TTAA 1 cut(s) 38
MslI CAYNNNNRTG 2 cut(s) 197, 287
MspA1I CMGCKG 2 cut(s) 93, 397
MspI CCGG 1 cut(s) 420
MunI CAATTG 1 cut(s) 310
Mva1269I GAATGC 1 cut(s) 22
MwoI GCNNNNNNNGC 2 cut(s) 64, 99
NarI GGCGCC 1 cut(s) 143
NcoI CCATGG 2 cut(s) 231, 300
NlaIII CATG 5 cut(s) 196, 235, 249, 286, 304
NlaIV GGNNCC 3 cut(s) 71, 144, 424
PctI GAATGC 1 cut(s) 22
PflFI GACNNNGTC 2 cut(s) 457, 494
PkrI GCNGC 6 cut(s) 69, 89, 92, 142, 396, 399
PleI GAGTC 1 cut(s) 466
PluTI GGCGCC 1 cut(s) 146
PpsI GAGTC 1 cut(s) 466
PshAI GACNNNNGTC 1 cut(s) 12
PspEI GGTNACC 1 cut(s) 415
PspFI CCCAGC 1 cut(s) 225
PspN4I GGNNCC 3 cut(s) 71, 144, 424
PspPI GGNCC 1 cut(s) 228
PstI CTGCAG 1 cut(s) 92
PsyI GACNNNGTC 2 cut(s) 457, 494
PvuII CAGCTG 1 cut(s) 93
RsaI GTAC 3 cut(s) 293, 330, 387
RsaNI GTAC 3 cut(s) 292, 329, 386
RseI CAYNNNNRTG 2 cut(s) 197, 287
SaqAI TTAA 1 cut(s) 38
SatI GCNGC 6 cut(s) 68, 88, 91, 141, 395, 398
Sau96I GGNCC 1 cut(s) 228
SchI GAGTC 1 cut(s) 467
SetI ASST 6 cut(s) 60, 95, 128, 244, 432, 498
SfaNI GCATC 1 cut(s) 127
SfcI CTRYAG 1 cut(s) 88
SfoI GGCGCC 1 cut(s) 144
SmiMI CAYNNNNRTG 2 cut(s) 197, 287
SmlI CTYRAG 1 cut(s) 160
SmoI CTYRAG 1 cut(s) 160
Sse9I AATT 3 cut(s) 168, 266, 310
SsiI CCGC 4 cut(s) 67, 140, 177, 397
SspDI GGCGCC 1 cut(s) 142
SspMI CTAG 2 cut(s) 27, 508
StyI CCWWGG 3 cut(s) 126, 231, 300
TaiI ACGT 1 cut(s) 498
TaqI TCGA 1 cut(s) 16
TasI AATT 3 cut(s) 168, 266, 310
TatI WGTACW 1 cut(s) 291
TauI GCSGC 3 cut(s) 70, 143, 400
Tru1I TTAA 1 cut(s) 38
Tru9I TTAA 1 cut(s) 38
TseI GCWGC 3 cut(s) 87, 90, 394
TspDTI ATGAA 3 cut(s) 181, 234, 497
TspGWI ACGGA 1 cut(s) 470
Tth111I GACNNNGTC 2 cut(s) 457, 494
XspI CTAG 2 cut(s) 27, 508
ZraI GACGTC 1 cut(s) 496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.