Rh2DG061100

Protein UPSTREAM OF FLC isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0007553
Physical Location & Seq
Reverse (-)
4495658 .. 4498333
2676 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG061100.1

Sequence Viewer

Length: 507 bp
ATGTACATCGGCAAGGATACAAGAACAGATTTTCAAAATCCAGGTTCAGATGAGGGAGATGACTGTCCCATGACCAAGCTGCCAGAAATCAACTTTGAGGCTCTGTTTTGGTGCAAAACTCCTAGAGAAATGAAGCTTATTTTTTGGCTCATATCACAGCCAACACCTTTCCTTGTAAGCTCTTCTCAAGGTCTCTACCTCAGAGATGTGATTAATAAGCTTAATGCTCTGAGGGGCAGAGGGATAGCATCTATGTATTCATGGTCCTCTAAGAGAAGCTATAAGAATGGGTATGTTTGGCATGATCTCAGTGAAGATGATGTAATTCTTCCAGCCCATGGAGACGAGTACGTCCTCAAAGGTTCTGAGATCTTTGATGAATCTAATTCAGATCGATTCAGTCCGATAGCAAATGTCAAAATCCAGAACTTGAAACAATTACCGGAACAAAAGAAAGGTGGAGGAGATGAGAGACGGGGAGGCGGAGCAGAGGGTGGAGGTCCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF966

Protein Analysis

168

Amino Acids

18.87

Weight (kDa)

5.46

Isoelectric Point (pI)

47.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SOK PF06136 59 - 125 2e-34 SOSEKI protein DIX-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 350
AccB7I CCANNNNNTGG 1 cut(s) 338
AciI CCGC 1 cut(s) 483
AfaI GTAC 2 cut(s) 5, 350
AfiI CCNNNNNNNGG 1 cut(s) 338
AgsI TTSAA 2 cut(s) 35, 433
AjnI CCWGG 1 cut(s) 40
AluBI AGCT 5 cut(s) 79, 136, 180, 220, 279
AluI AGCT 5 cut(s) 79, 136, 180, 220, 279
Alw26I GTCTC 3 cut(s) 197, 336, 466
ApeKI GCWGC 1 cut(s) 79
AseI ATTAAT 1 cut(s) 213
AspS9I GGNCC 2 cut(s) 264, 500
AvaII GGWCC 2 cut(s) 264, 500
BbvI GCAGC 1 cut(s) 66
BciT130I CCWGG 1 cut(s) 42
BciVI GTATCC 1 cut(s) 10
BcoDI GTCTC 3 cut(s) 197, 336, 466
BfaI CTAG 1 cut(s) 123
BfuI GTATCC 1 cut(s) 10
BglII AGATCT 1 cut(s) 369
BisI GCNGC 1 cut(s) 80
BlsI GCNGC 1 cut(s) 81
Bme1390I CCNGG 1 cut(s) 42
Bme18I GGWCC 2 cut(s) 264, 500
BmgT120I GGNCC 2 cut(s) 264, 500
BmrFI CCNGG 1 cut(s) 42
BmsI GCATC 1 cut(s) 257
BpuEI CTTGAG 1 cut(s) 171
Bsa29I ATCGAT 1 cut(s) 394
BsaI GGTCTC 1 cut(s) 197
BsaJI CCNNGG 1 cut(s) 337
BsaWI WCCGGW 1 cut(s) 442
Bsc4I CCNNNNNNNGG 1 cut(s) 338
BseBI CCWGG 1 cut(s) 42
BseCI ATCGAT 1 cut(s) 394
BseDI CCNNGG 1 cut(s) 337
BseLI CCNNNNNNNGG 1 cut(s) 338
BseMII CTCAG 4 cut(s) 214, 221, 322, 357
BseRI GAGGAG 1 cut(s) 477
BseXI GCAGC 1 cut(s) 66
BshVI ATCGAT 1 cut(s) 394
BsiSI CCGG 1 cut(s) 443
BslFI GGGAC 1 cut(s) 51
BslI CCNNNNNNNGG 1 cut(s) 338
BsmAI GTCTC 3 cut(s) 197, 336, 466
BsmBI CGTCTC 2 cut(s) 336, 466
BsmFI GGGAC 1 cut(s) 51
Bso31I GGTCTC 1 cut(s) 197
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 3 cut(s) 304, 369, 391
Bsp19I CCATGG 1 cut(s) 337
BspACI CCGC 1 cut(s) 483
BspCNI CTCAG 4 cut(s) 213, 222, 321, 358
BspDI ATCGAT 1 cut(s) 394
BspQI GCTCTTC 1 cut(s) 187
BspTNI GGTCTC 1 cut(s) 197
BsrGI TGTACA 1 cut(s) 3
BssECI CCNNGG 1 cut(s) 337
BssMI GATC 3 cut(s) 304, 369, 391
BssT1I CCWWGG 1 cut(s) 337
Bst2UI CCWGG 1 cut(s) 42
Bst4CI ACNGT 1 cut(s) 65
Bst6I CTCTTC 1 cut(s) 187
BstAUI TGTACA 1 cut(s) 3
BstDEI CTNAG 5 cut(s) 200, 230, 270, 308, 366
BstDSI CCRYGG 1 cut(s) 337
BstKTI GATC 3 cut(s) 307, 372, 394
BstMAI GTCTC 3 cut(s) 197, 336, 466
BstMBI GATC 3 cut(s) 304, 369, 391
BstNI CCWGG 1 cut(s) 42
BstSCI CCNGG 1 cut(s) 40
BstV1I GCAGC 1 cut(s) 66
BstX2I RGATCY 1 cut(s) 369
BstYI RGATCY 1 cut(s) 369
Bsu15I ATCGAT 1 cut(s) 394
BsuI GTATCC 1 cut(s) 10
BsuTUI ATCGAT 1 cut(s) 394
BtgI CCRYGG 1 cut(s) 337
BtsIMutI CAGTG 1 cut(s) 316
Cfr13I GGNCC 2 cut(s) 264, 500
ClaI ATCGAT 1 cut(s) 394
Csp6I GTAC 2 cut(s) 4, 349
CviAII CATG 4 cut(s) 70, 261, 302, 338
CviJI RGCY 9 cut(s) 79, 101, 136, 148, 160, 180, 220, 279, 335
CviKI_1 RGCY 9 cut(s) 79, 101, 136, 148, 160, 180, 220, 279, 335
CviQI GTAC 2 cut(s) 4, 349
DdeI CTNAG 5 cut(s) 200, 230, 270, 308, 366
DpnI GATC 3 cut(s) 306, 371, 393
DpnII GATC 3 cut(s) 304, 369, 391
DrdI GACNNNNNNGTC 1 cut(s) 350
DseDI GACNNNNNNGTC 1 cut(s) 350
Eam1104I CTCTTC 1 cut(s) 187
EarI CTCTTC 1 cut(s) 187
EciI GGCGGA 1 cut(s) 498
Eco130I CCWWGG 1 cut(s) 337
Eco31I GGTCTC 1 cut(s) 197
Eco47I GGWCC 2 cut(s) 264, 500
EcoRII CCWGG 1 cut(s) 40
EcoT14I CCWWGG 1 cut(s) 337
ErhI CCWWGG 1 cut(s) 337
Esp3I CGTCTC 2 cut(s) 336, 466
FaeI CATG 4 cut(s) 73, 264, 305, 341
FaiI YATR 8 cut(s) 71, 152, 254, 262, 282, 294, 303, 339
FaqI GGGAC 1 cut(s) 51
FatI CATG 4 cut(s) 69, 260, 301, 337
Fnu4HI GCNGC 1 cut(s) 80
Fsp4HI GCNGC 1 cut(s) 80
FspBI CTAG 1 cut(s) 123
GluI GCNGC 1 cut(s) 80
HapII CCGG 1 cut(s) 443
Hin1II CATG 4 cut(s) 73, 264, 305, 341
HindIII AAGCTT 2 cut(s) 134, 218
HinfI GANTC 2 cut(s) 380, 396
HpaII CCGG 1 cut(s) 443
Hpy188I TCNGA 6 cut(s) 49, 203, 231, 367, 391, 405
Hpy188III TCNNGA 1 cut(s) 424
HpyCH4III ACNGT 1 cut(s) 65
HpyCH4IV ACGT 1 cut(s) 351
HpyCH4V TGCA 1 cut(s) 114
HpyF3I CTNAG 5 cut(s) 200, 230, 270, 308, 366
HpySE526I ACGT 1 cut(s) 351
Hsp92II CATG 4 cut(s) 73, 264, 305, 341
Kzo9I GATC 3 cut(s) 304, 369, 391
LguI GCTCTTC 1 cut(s) 187
LmnI GCTCC 1 cut(s) 485
LpnPI CCDG 6 cut(s) 27, 54, 96, 345, 437, 456
Lsp1109I GCAGC 1 cut(s) 66
LweI GCATC 1 cut(s) 257
MaeI CTAG 1 cut(s) 123
MaeII ACGT 1 cut(s) 351
MalI GATC 3 cut(s) 306, 371, 393
MboI GATC 3 cut(s) 304, 369, 391
MboII GAAGA 3 cut(s) 174, 320, 326
MflI RGATCY 1 cut(s) 369
MluCI AATT 3 cut(s) 324, 385, 437
MseI TTAA 2 cut(s) 213, 222
MspI CCGG 1 cut(s) 443
MspR9I CCNGG 1 cut(s) 42
MvaI CCWGG 1 cut(s) 42
NcoI CCATGG 1 cut(s) 337
NdeII GATC 3 cut(s) 304, 369, 391
NlaIII CATG 4 cut(s) 73, 264, 305, 341
PciSI GCTCTTC 1 cut(s) 187
PfeI GAWTC 2 cut(s) 380, 396
PflMI CCANNNNNTGG 1 cut(s) 338
PkrI GCNGC 1 cut(s) 81
PshBI ATTAAT 1 cut(s) 213
Psp6I CCWGG 1 cut(s) 40
PspGI CCWGG 1 cut(s) 40
PspPI GGNCC 2 cut(s) 264, 500
PsuI RGATCY 1 cut(s) 369
RsaI GTAC 2 cut(s) 5, 350
RsaNI GTAC 2 cut(s) 4, 349
SapI GCTCTTC 1 cut(s) 187
SaqAI TTAA 2 cut(s) 213, 222
SatI GCNGC 1 cut(s) 80
Sau3AI GATC 3 cut(s) 304, 369, 391
Sau96I GGNCC 2 cut(s) 264, 500
ScrFI CCNGG 1 cut(s) 42
SfaNI GCATC 1 cut(s) 257
SinI GGWCC 2 cut(s) 264, 500
SmlI CTYRAG 1 cut(s) 186
SmoI CTYRAG 1 cut(s) 186
Sse9I AATT 3 cut(s) 324, 385, 437
SsiI CCGC 1 cut(s) 483
SspMI CTAG 1 cut(s) 123
StyD4I CCNGG 1 cut(s) 40
StyI CCWWGG 1 cut(s) 337
TaaI ACNGT 1 cut(s) 65
TaiI ACGT 1 cut(s) 354
TaqI TCGA 1 cut(s) 394
TasI AATT 3 cut(s) 324, 385, 437
TatI WGTACW 1 cut(s) 3
TfiI GAWTC 2 cut(s) 380, 396
Tru1I TTAA 2 cut(s) 213, 222
Tru9I TTAA 2 cut(s) 213, 222
TscAI CASTG 1 cut(s) 316
TseI GCWGC 1 cut(s) 79
TspDTI ATGAA 3 cut(s) 146, 249, 393
TspGWI ACGGA 1 cut(s) 492
TspRI CASTG 1 cut(s) 316
Van91I CCANNNNNTGG 1 cut(s) 338
VpaK11BI GGWCC 2 cut(s) 264, 500
VspI ATTAAT 1 cut(s) 213
XspI CTAG 1 cut(s) 123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.