Rh5CG486200

Protein UPSTREAM OF FLC isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0007553
Physical Location & Seq
Forward (+)
67833038 .. 67836075
3038 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG486200.1

Sequence Viewer

Length: 648 bp
ATGGCTGCTTCAAGGAGATCGTGGTGGTGGTTTGGTGTTCTATGGCAGATTGATGGGCCATGGTTGAAAGTTCATCAATATCTGAATCAGATCAGGTCAATCTTAAAGGTTTTGGGTGGATCTGGGTTTGGATTATTGGGGACAATATTCAAGGGTTGGGTTTATGAGAGAAGGGGATACATGAATTGGCAGCGGTGGGCACAGTGGTTGGTGGTGTGCGGCTTTGTGGTCTACAATTGTTGGCTTATTCTGATATCTTCAAGCTTGGCGTGGTCTGTTCGGAACCTGCAAAGATCACAGCCAACACCTTTCCTTGTAAGCTCTTCTCAAGGTCTCTACCTCAGAGATGTGATTAATAAGCTTAATGCTCTGAGGGGCAGAGGGATAGCATCTATGTATTCATGGTCCTCTAAGAGAAGCTATAAGAATGGGTATGTTTGGCATGATCTCAGTGAAGATGATGTAATTCTTCCGGCCCATGGAGACGAGTACGTCCTCAAAGGTTCTGAGATCTTTGATGAATCTAATTCAGATCGATTCAGTCCGATAGCAAATGTCAAAATCCAGAACTTGAAACAATTACCGGAACCAAAGAAAGGTGGAGGAGATGAGAGACGGGGAGGCGGAGCAGAGGGTGGAGGTCCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF966

Protein Analysis

215

Amino Acids

24.49

Weight (kDa)

9.66

Isoelectric Point (pI)

58.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SOK PF06136 105 - 172 2e-34 SOSEKI protein DIX-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 491
Acc36I ACCTGC 1 cut(s) 294
AccI GTMKAC 1 cut(s) 231
AciI CCGC 3 cut(s) 193, 219, 624
AclWI GGATC 1 cut(s) 127
AfaI GTAC 1 cut(s) 491
AfiI CCNNNNNNNGG 2 cut(s) 479, 596
AgsI TTSAA 5 cut(s) 12, 67, 151, 261, 574
AluBI AGCT 4 cut(s) 264, 321, 361, 420
AluI AGCT 4 cut(s) 264, 321, 361, 420
Alw26I GTCTC 3 cut(s) 338, 477, 607
AlwI GGATC 1 cut(s) 127
AoxI GGCC 2 cut(s) 56, 474
ApeKI GCWGC 2 cut(s) 5, 190
AseI ATTAAT 1 cut(s) 354
AspS9I GGNCC 4 cut(s) 56, 405, 475, 641
AvaII GGWCC 2 cut(s) 405, 641
BaeGI GKGCMC 1 cut(s) 202
BbvI GCAGC 1 cut(s) 202
BccI CCATC 1 cut(s) 47
BciVI GTATCC 1 cut(s) 170
BcoDI GTCTC 3 cut(s) 338, 477, 607
BfuAI ACCTGC 1 cut(s) 294
BfuI GTATCC 1 cut(s) 170
BglII AGATCT 1 cut(s) 510
BisI GCNGC 3 cut(s) 6, 191, 220
BlsI GCNGC 3 cut(s) 7, 192, 221
Bme18I GGWCC 2 cut(s) 405, 641
BmgT120I GGNCC 4 cut(s) 56, 405, 475, 641
BmiI GGNNCC 2 cut(s) 284, 588
BmsI GCATC 1 cut(s) 398
BpuEI CTTGAG 1 cut(s) 312
Bsa29I ATCGAT 1 cut(s) 535
BsaI GGTCTC 1 cut(s) 338
BsaJI CCNNGG 2 cut(s) 59, 478
BsaWI WCCGGW 1 cut(s) 583
Bsc4I CCNNNNNNNGG 2 cut(s) 479, 596
BseCI ATCGAT 1 cut(s) 535
BseDI CCNNGG 2 cut(s) 59, 478
BseLI CCNNNNNNNGG 2 cut(s) 479, 596
BseMII CTCAG 4 cut(s) 355, 362, 463, 498
BseRI GAGGAG 1 cut(s) 618
BseSI GKGCMC 1 cut(s) 202
BseXI GCAGC 1 cut(s) 202
BshFI GGCC 2 cut(s) 58, 476
BshVI ATCGAT 1 cut(s) 535
BsiSI CCGG 2 cut(s) 473, 584
BslFI GGGAC 1 cut(s) 154
BslI CCNNNNNNNGG 2 cut(s) 479, 596
BsmAI GTCTC 3 cut(s) 338, 477, 607
BsmBI CGTCTC 2 cut(s) 477, 607
BsmFI GGGAC 1 cut(s) 154
BsnI GGCC 2 cut(s) 58, 476
Bso31I GGTCTC 1 cut(s) 338
Bsp1286I GDGCHC 1 cut(s) 202
Bsp143I GATC 7 cut(s) 17, 90, 119, 293, 445, 510, 532
Bsp19I CCATGG 2 cut(s) 59, 478
BspACI CCGC 3 cut(s) 193, 219, 624
BspANI GGCC 2 cut(s) 58, 476
BspCNI CTCAG 4 cut(s) 354, 363, 462, 499
BspDI ATCGAT 1 cut(s) 535
BspLI GGNNCC 2 cut(s) 284, 588
BspMI ACCTGC 1 cut(s) 294
BspPI GGATC 1 cut(s) 127
BspQI GCTCTTC 1 cut(s) 328
BspTNI GGTCTC 1 cut(s) 338
BssECI CCNNGG 2 cut(s) 59, 478
BssMI GATC 7 cut(s) 17, 90, 119, 293, 445, 510, 532
BssT1I CCWWGG 2 cut(s) 59, 478
Bst4CI ACNGT 1 cut(s) 204
Bst6I CTCTTC 1 cut(s) 328
BstDEI CTNAG 5 cut(s) 341, 371, 411, 449, 507
BstDSI CCRYGG 2 cut(s) 59, 478
BstKTI GATC 7 cut(s) 20, 93, 122, 296, 448, 513, 535
BstMAI GTCTC 3 cut(s) 338, 477, 607
BstMBI GATC 7 cut(s) 17, 90, 119, 293, 445, 510, 532
BstSLI GKGCMC 1 cut(s) 202
BstV1I GCAGC 1 cut(s) 202
BstX2I RGATCY 2 cut(s) 119, 510
BstYI RGATCY 2 cut(s) 119, 510
Bsu15I ATCGAT 1 cut(s) 535
BsuI GTATCC 1 cut(s) 170
BsuRI GGCC 2 cut(s) 58, 476
BsuTUI ATCGAT 1 cut(s) 535
BtgI CCRYGG 2 cut(s) 59, 478
BtsIMutI CAGTG 2 cut(s) 209, 457
BveI ACCTGC 1 cut(s) 294
Cfr13I GGNCC 4 cut(s) 56, 405, 475, 641
ClaI ATCGAT 1 cut(s) 535
Csp6I GTAC 1 cut(s) 490
CviAII CATG 5 cut(s) 60, 181, 402, 443, 479
CviQI GTAC 1 cut(s) 490
DdeI CTNAG 5 cut(s) 341, 371, 411, 449, 507
DpnI GATC 7 cut(s) 19, 92, 121, 295, 447, 512, 534
DpnII GATC 7 cut(s) 17, 90, 119, 293, 445, 510, 532
DrdI GACNNNNNNGTC 1 cut(s) 491
DseDI GACNNNNNNGTC 1 cut(s) 491
Eam1104I CTCTTC 1 cut(s) 328
EarI CTCTTC 1 cut(s) 328
EciI GGCGGA 1 cut(s) 639
Eco130I CCWWGG 2 cut(s) 59, 478
Eco31I GGTCTC 1 cut(s) 338
Eco32I GATATC 1 cut(s) 255
Eco47I GGWCC 2 cut(s) 405, 641
EcoRV GATATC 1 cut(s) 255
EcoT14I CCWWGG 2 cut(s) 59, 478
ErhI CCWWGG 2 cut(s) 59, 478
Esp3I CGTCTC 2 cut(s) 477, 607
FaeI CATG 5 cut(s) 63, 184, 405, 446, 482
FaqI GGGAC 1 cut(s) 154
FatI CATG 5 cut(s) 59, 180, 401, 442, 478
FblI GTMKAC 1 cut(s) 231
Fnu4HI GCNGC 3 cut(s) 6, 191, 220
Fsp4HI GCNGC 3 cut(s) 6, 191, 220
GluI GCNGC 3 cut(s) 6, 191, 220
HaeIII GGCC 2 cut(s) 58, 476
HapII CCGG 2 cut(s) 473, 584
Hin1II CATG 5 cut(s) 63, 184, 405, 446, 482
HindIII AAGCTT 2 cut(s) 262, 359
HinfI GANTC 3 cut(s) 85, 521, 537
HpaII CCGG 2 cut(s) 473, 584
Hpy166II GTNNAC 1 cut(s) 232
Hpy188I TCNGA 9 cut(s) 84, 90, 252, 282, 344, 372, 508, 532, 546
Hpy188III TCNNGA 1 cut(s) 565
Hpy8I GTNNAC 1 cut(s) 232
HpyAV CCTTC 1 cut(s) 165
HpyCH4III ACNGT 1 cut(s) 204
HpyCH4IV ACGT 1 cut(s) 492
HpyCH4V TGCA 1 cut(s) 289
HpyF3I CTNAG 5 cut(s) 341, 371, 411, 449, 507
HpySE526I ACGT 1 cut(s) 492
Hsp92II CATG 5 cut(s) 63, 184, 405, 446, 482
Kzo9I GATC 7 cut(s) 17, 90, 119, 293, 445, 510, 532
LguI GCTCTTC 1 cut(s) 328
LmnI GCTCC 1 cut(s) 626
LpnPI CCDG 6 cut(s) 79, 108, 299, 486, 578, 597
Lsp1109I GCAGC 1 cut(s) 202
LweI GCATC 1 cut(s) 398
MaeII ACGT 1 cut(s) 492
MalI GATC 7 cut(s) 19, 92, 121, 295, 447, 512, 534
MboI GATC 7 cut(s) 17, 90, 119, 293, 445, 510, 532
MboII GAAGA 4 cut(s) 249, 315, 461, 467
MfeI CAATTG 1 cut(s) 235
MflI RGATCY 2 cut(s) 119, 510
MhlI GDGCHC 1 cut(s) 202
MluCI AATT 5 cut(s) 184, 235, 465, 526, 578
MnlI CCTC 9 cut(s) 350, 366, 374, 418, 506, 596, 614, 625, 632
MseI TTAA 3 cut(s) 104, 354, 363
MspA1I CMGCKG 1 cut(s) 193
MspI CCGG 2 cut(s) 473, 584
MunI CAATTG 1 cut(s) 235
NcoI CCATGG 2 cut(s) 59, 478
NdeII GATC 7 cut(s) 17, 90, 119, 293, 445, 510, 532
NlaIII CATG 5 cut(s) 63, 184, 405, 446, 482
NlaIV GGNNCC 2 cut(s) 284, 588
PciSI GCTCTTC 1 cut(s) 328
PfeI GAWTC 3 cut(s) 85, 521, 537
PkrI GCNGC 3 cut(s) 7, 192, 221
PshBI ATTAAT 1 cut(s) 354
PspN4I GGNNCC 2 cut(s) 284, 588
PspPI GGNCC 4 cut(s) 56, 405, 475, 641
PsuI RGATCY 2 cut(s) 119, 510
RsaI GTAC 1 cut(s) 491
RsaNI GTAC 1 cut(s) 490
SapI GCTCTTC 1 cut(s) 328
SaqAI TTAA 3 cut(s) 104, 354, 363
SatI GCNGC 3 cut(s) 6, 191, 220
Sau3AI GATC 7 cut(s) 17, 90, 119, 293, 445, 510, 532
Sau96I GGNCC 4 cut(s) 56, 405, 475, 641
SduI GDGCHC 1 cut(s) 202
SfaNI GCATC 1 cut(s) 398
SinI GGWCC 2 cut(s) 405, 641
SmlI CTYRAG 1 cut(s) 327
SmoI CTYRAG 1 cut(s) 327
Sse9I AATT 5 cut(s) 184, 235, 465, 526, 578
SsiI CCGC 3 cut(s) 193, 219, 624
SspI AATATT 1 cut(s) 147
StyI CCWWGG 2 cut(s) 59, 478
TaaI ACNGT 1 cut(s) 204
TaiI ACGT 1 cut(s) 495
TaqI TCGA 1 cut(s) 535
TasI AATT 5 cut(s) 184, 235, 465, 526, 578
TauI GCSGC 1 cut(s) 222
TfiI GAWTC 3 cut(s) 85, 521, 537
Tru1I TTAA 3 cut(s) 104, 354, 363
Tru9I TTAA 3 cut(s) 104, 354, 363
TscAI CASTG 2 cut(s) 209, 457
TseI GCWGC 2 cut(s) 5, 190
TspDTI ATGAA 4 cut(s) 62, 197, 390, 534
TspGWI ACGGA 1 cut(s) 633
TspRI CASTG 2 cut(s) 209, 457
VpaK11BI GGWCC 2 cut(s) 405, 641
VspI ATTAAT 1 cut(s) 354
XmiI GTMKAC 1 cut(s) 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.