Rh5DG271800

Protein UPSTREAM OF FLC isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0007553
Physical Location & Seq
Forward (+)
33054793 .. 33056577
1785 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG271800.1

Sequence Viewer

Length: 396 bp
ATGGTTGATATGAGTAACAGCTTCGTCGTCCTCAATATCGTCTTCGAGCCAACACCTTTCCTTGTAAGCTCTTCTCAAGGTCTCTACCTCAGAGATGTGATTAATAAGCTTAATGCTCTGAGGGGCAGAGGGATAGCATCTATGTATTCATGGTCCTCTAAGAGAAGCTATAAGAATGGGTATGTTTGGCATGATCTCAGTGAAGATGATGTAATTCTTCCGGCCCATGGAGACGAGTACGTCCTCAAAGGTTCTGAGATCTTTGATGAATCTAATTCAGATCGATTCAGTCCGATAGCAAATGTCAAAATCCAGAACTTGAAACAATTACCGGAACCAAAGAAAGGTGGAGGAGATGAGAGACGGGGAGGCGGAGCAGAGGGTGGAGGTCCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF966

Protein Analysis

131

Amino Acids

14.42

Weight (kDa)

5.66

Isoelectric Point (pI)

58.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SOK PF06136 22 - 88 1e-34 SOSEKI protein DIX-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 239
AciI CCGC 1 cut(s) 372
AfaI GTAC 1 cut(s) 239
AfiI CCNNNNNNNGG 2 cut(s) 227, 344
AgsI TTSAA 1 cut(s) 322
AluBI AGCT 4 cut(s) 21, 69, 109, 168
AluI AGCT 4 cut(s) 21, 69, 109, 168
Alw26I GTCTC 3 cut(s) 86, 225, 355
AoxI GGCC 1 cut(s) 222
AseI ATTAAT 1 cut(s) 102
AspS9I GGNCC 3 cut(s) 153, 223, 389
AvaII GGWCC 2 cut(s) 153, 389
BbsI GAAGAC 1 cut(s) 34
BcoDI GTCTC 3 cut(s) 86, 225, 355
BglII AGATCT 1 cut(s) 258
Bme18I GGWCC 2 cut(s) 153, 389
BmgT120I GGNCC 3 cut(s) 153, 223, 389
BmiI GGNNCC 1 cut(s) 336
BmsI GCATC 1 cut(s) 146
BpiI GAAGAC 1 cut(s) 34
BpuEI CTTGAG 1 cut(s) 60
Bsa29I ATCGAT 1 cut(s) 283
BsaI GGTCTC 1 cut(s) 86
BsaJI CCNNGG 1 cut(s) 226
BsaWI WCCGGW 1 cut(s) 331
Bsc4I CCNNNNNNNGG 2 cut(s) 227, 344
BseCI ATCGAT 1 cut(s) 283
BseDI CCNNGG 1 cut(s) 226
BseLI CCNNNNNNNGG 2 cut(s) 227, 344
BseMII CTCAG 4 cut(s) 103, 110, 211, 246
BseRI GAGGAG 1 cut(s) 366
BshFI GGCC 1 cut(s) 224
BshVI ATCGAT 1 cut(s) 283
BsiSI CCGG 2 cut(s) 221, 332
BslI CCNNNNNNNGG 2 cut(s) 227, 344
BsmAI GTCTC 3 cut(s) 86, 225, 355
BsmBI CGTCTC 2 cut(s) 225, 355
BsnI GGCC 1 cut(s) 224
Bso31I GGTCTC 1 cut(s) 86
Bsp143I GATC 3 cut(s) 193, 258, 280
Bsp19I CCATGG 1 cut(s) 226
BspACI CCGC 1 cut(s) 372
BspANI GGCC 1 cut(s) 224
BspCNI CTCAG 4 cut(s) 102, 111, 210, 247
BspDI ATCGAT 1 cut(s) 283
BspLI GGNNCC 1 cut(s) 336
BspQI GCTCTTC 1 cut(s) 76
BspTNI GGTCTC 1 cut(s) 86
BssECI CCNNGG 1 cut(s) 226
BssMI GATC 3 cut(s) 193, 258, 280
BssT1I CCWWGG 1 cut(s) 226
Bst6I CTCTTC 1 cut(s) 76
BstDEI CTNAG 5 cut(s) 89, 119, 159, 197, 255
BstDSI CCRYGG 1 cut(s) 226
BstKTI GATC 3 cut(s) 196, 261, 283
BstMAI GTCTC 3 cut(s) 86, 225, 355
BstMBI GATC 3 cut(s) 193, 258, 280
BstV2I GAAGAC 1 cut(s) 34
BstX2I RGATCY 1 cut(s) 258
BstYI RGATCY 1 cut(s) 258
Bsu15I ATCGAT 1 cut(s) 283
BsuRI GGCC 1 cut(s) 224
BsuTUI ATCGAT 1 cut(s) 283
BtgI CCRYGG 1 cut(s) 226
BtsIMutI CAGTG 1 cut(s) 205
Cfr13I GGNCC 3 cut(s) 153, 223, 389
ClaI ATCGAT 1 cut(s) 283
Csp6I GTAC 1 cut(s) 238
CviAII CATG 3 cut(s) 150, 191, 227
CviJI RGCY 6 cut(s) 21, 49, 69, 109, 168, 224
CviKI_1 RGCY 6 cut(s) 21, 49, 69, 109, 168, 224
CviQI GTAC 1 cut(s) 238
DdeI CTNAG 5 cut(s) 89, 119, 159, 197, 255
DpnI GATC 3 cut(s) 195, 260, 282
DpnII GATC 3 cut(s) 193, 258, 280
DrdI GACNNNNNNGTC 1 cut(s) 239
DseDI GACNNNNNNGTC 1 cut(s) 239
Eam1104I CTCTTC 1 cut(s) 76
EarI CTCTTC 1 cut(s) 76
EciI GGCGGA 1 cut(s) 387
Eco130I CCWWGG 1 cut(s) 226
Eco31I GGTCTC 1 cut(s) 86
Eco47I GGWCC 2 cut(s) 153, 389
EcoT14I CCWWGG 1 cut(s) 226
ErhI CCWWGG 1 cut(s) 226
Esp3I CGTCTC 2 cut(s) 225, 355
FaeI CATG 3 cut(s) 153, 194, 230
FaiI YATR 7 cut(s) 11, 143, 151, 171, 183, 192, 228
FatI CATG 3 cut(s) 149, 190, 226
HaeIII GGCC 1 cut(s) 224
HapII CCGG 2 cut(s) 221, 332
Hin1II CATG 3 cut(s) 153, 194, 230
HindIII AAGCTT 1 cut(s) 107
HinfI GANTC 2 cut(s) 269, 285
HpaII CCGG 2 cut(s) 221, 332
Hpy188I TCNGA 5 cut(s) 92, 120, 256, 280, 294
Hpy188III TCNNGA 1 cut(s) 313
Hpy99I CGWCG 1 cut(s) 29
HpyCH4IV ACGT 1 cut(s) 240
HpyF3I CTNAG 5 cut(s) 89, 119, 159, 197, 255
HpySE526I ACGT 1 cut(s) 240
Hsp92II CATG 3 cut(s) 153, 194, 230
Kzo9I GATC 3 cut(s) 193, 258, 280
LguI GCTCTTC 1 cut(s) 76
LmnI GCTCC 1 cut(s) 374
LpnPI CCDG 3 cut(s) 234, 326, 345
LweI GCATC 1 cut(s) 146
MaeII ACGT 1 cut(s) 240
MaeIII GTNAC 1 cut(s) 14
MalI GATC 3 cut(s) 195, 260, 282
MboI GATC 3 cut(s) 193, 258, 280
MboII GAAGA 4 cut(s) 34, 63, 209, 215
MflI RGATCY 1 cut(s) 258
MluCI AATT 3 cut(s) 213, 274, 326
MseI TTAA 2 cut(s) 102, 111
MspI CCGG 2 cut(s) 221, 332
NcoI CCATGG 1 cut(s) 226
NdeII GATC 3 cut(s) 193, 258, 280
NlaIII CATG 3 cut(s) 153, 194, 230
NlaIV GGNNCC 1 cut(s) 336
PciSI GCTCTTC 1 cut(s) 76
PfeI GAWTC 2 cut(s) 269, 285
PshBI ATTAAT 1 cut(s) 102
PspN4I GGNNCC 1 cut(s) 336
PspPI GGNCC 3 cut(s) 153, 223, 389
PsuI RGATCY 1 cut(s) 258
RsaI GTAC 1 cut(s) 239
RsaNI GTAC 1 cut(s) 238
SapI GCTCTTC 1 cut(s) 76
SaqAI TTAA 2 cut(s) 102, 111
Sau3AI GATC 3 cut(s) 193, 258, 280
Sau96I GGNCC 3 cut(s) 153, 223, 389
SfaNI GCATC 1 cut(s) 146
SinI GGWCC 2 cut(s) 153, 389
SmlI CTYRAG 1 cut(s) 75
SmoI CTYRAG 1 cut(s) 75
Sse9I AATT 3 cut(s) 213, 274, 326
SsiI CCGC 1 cut(s) 372
StyI CCWWGG 1 cut(s) 226
TaiI ACGT 1 cut(s) 243
TaqI TCGA 2 cut(s) 45, 283
TasI AATT 3 cut(s) 213, 274, 326
TfiI GAWTC 2 cut(s) 269, 285
Tru1I TTAA 2 cut(s) 102, 111
Tru9I TTAA 2 cut(s) 102, 111
TscAI CASTG 1 cut(s) 205
TspDTI ATGAA 2 cut(s) 138, 282
TspGWI ACGGA 1 cut(s) 381
TspRI CASTG 1 cut(s) 205
VpaK11BI GGWCC 2 cut(s) 153, 389
VspI ATTAAT 1 cut(s) 102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.