RLG00000002093

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0003488
Physical Location & Seq
Reverse (-)
25305676 .. 25306434
759 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002093

Sequence Viewer

Length: 615 bp
ATGCAGGGCCTCCACCACAAATACCATCATGTCATTAAGTACGGCTATTATCAGTCTGTAATTCTAACCATTGAGGGCGTCCGTGGAAAACAATGGCATGTCCGGCTATTTCATGATGGAACCGGTAACTTTTGGTTGATGGATGGATGGGCAAGATTCCATAATGATAACGAGCTTGGCAACAATGAGCTACTCCAGTTCAAGTTTGATGATGCTACTGGCAATGTAGCCGCAATCATTATTGTTCAAGTGGAGGAAGTGGTTAATTATGAGTTGGAGACCGATGATGAGATGGGATGTGATACTTACGATAACCACGCCATGCAGCGCATGCGACCTATTGGGGTTGAATTTAAAACACACATTCGTCGTAAAGATTTGAACATTCCGACATGTATTGTTAAGGAATATCTGAAGATCATCGAACAAAATTTTACTATCATAGACCTAGCAAATGTGCATTGGCGAGTGGGTGGTATTCCTTCCAATACCAATGTGAAGCTTTCCAGCGGGTGGCCTGCGATTGTGAAAAGCCGGGTGATTAAAGAAGGAGATCATGTTCAATTGGTTTATATAGGTGAGAAGACCTTTAAGATATTCAAACTCAATTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

205

Amino Acids

23.76

Weight (kDa)

6.59

Isoelectric Point (pI)

24.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 10 - 74 1.3e-09 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 513
AciI CCGC 2 cut(s) 231, 510
AcsI RAATTY 2 cut(s) 350, 430
AcuI CTGAAG 1 cut(s) 434
AcyI GRCGYC 1 cut(s) 78
AfaI GTAC 1 cut(s) 41
AfiI CCNNNNNNNGG 1 cut(s) 513
AflIII ACRYGT 1 cut(s) 392
AgeI ACCGGT 1 cut(s) 122
AgsI TTSAA 6 cut(s) 202, 248, 350, 382, 563, 601
AloI GAACNNNNNNTCC 2 cut(s) 543, 575
AluBI AGCT 3 cut(s) 175, 190, 502
AluI AGCT 3 cut(s) 175, 190, 502
Alw26I GTCTC 1 cut(s) 272
AoxI GGCC 2 cut(s) 7, 515
ApeKI GCWGC 1 cut(s) 325
ApoI RAATTY 2 cut(s) 350, 430
AsiGI ACCGGT 1 cut(s) 122
AspLEI GCGC 1 cut(s) 330
AspS9I GGNCC 1 cut(s) 7
AsuC2I CCSGG 1 cut(s) 536
AsuHPI GGTGA 2 cut(s) 550, 590
BbsI GAAGAC 1 cut(s) 590
BbvI GCAGC 1 cut(s) 337
BccI CCATC 6 cut(s) 33, 110, 133, 137, 141, 286
BceAI ACGGC 1 cut(s) 58
BcnI CCSGG 1 cut(s) 536
BcoDI GTCTC 1 cut(s) 272
BfaI CTAG 1 cut(s) 449
BisI GCNGC 2 cut(s) 231, 326
BlsI GCNGC 2 cut(s) 232, 327
Bme1390I CCNGG 1 cut(s) 536
BmgT120I GGNCC 1 cut(s) 7
BmiI GGNNCC 1 cut(s) 121
BmrFI CCNGG 1 cut(s) 536
BmsI GCATC 1 cut(s) 202
BpiI GAAGAC 1 cut(s) 590
BpmI CTGGAG 1 cut(s) 179
BpuMI CCSGG 1 cut(s) 536
BsaHI GRCGYC 1 cut(s) 78
BsaI GGTCTC 1 cut(s) 272
BsaJI CCNNGG 1 cut(s) 82
BsaWI WCCGGW 1 cut(s) 122
BsaXI ACNNNNNCTCC 2 cut(s) 543, 573
Bsc4I CCNNNNNNNGG 1 cut(s) 513
Bse118I RCCGGY 1 cut(s) 122
Bse1I ACTGG 2 cut(s) 196, 223
Bse3DI GCAATG 1 cut(s) 229
BseDI CCNNGG 1 cut(s) 82
BseGI GGATG 3 cut(s) 148, 152, 302
BseLI CCNNNNNNNGG 1 cut(s) 513
BseMI GCAATG 1 cut(s) 229
BseNI ACTGG 2 cut(s) 196, 223
BseXI GCAGC 1 cut(s) 337
BshFI GGCC 2 cut(s) 9, 517
BshTI ACCGGT 1 cut(s) 122
BsiSI CCGG 3 cut(s) 103, 123, 535
BslI CCNNNNNNNGG 1 cut(s) 513
BsmAI GTCTC 1 cut(s) 272
BsnI GGCC 2 cut(s) 9, 517
Bso31I GGTCTC 1 cut(s) 272
Bsp143I GATC 2 cut(s) 417, 553
BspACI CCGC 2 cut(s) 231, 510
BspANI GGCC 2 cut(s) 9, 517
BspHI TCATGA 1 cut(s) 112
BspLI GGNNCC 1 cut(s) 121
BspTNI GGTCTC 1 cut(s) 272
BsrDI GCAATG 1 cut(s) 229
BsrFI RCCGGY 1 cut(s) 122
BsrI ACTGG 2 cut(s) 196, 223
BssAI RCCGGY 1 cut(s) 122
BssECI CCNNGG 1 cut(s) 82
BssMI GATC 2 cut(s) 417, 553
BssNI GRCGYC 1 cut(s) 78
BstACI GRCGYC 1 cut(s) 78
BstAPI GCANNNNNTGC 1 cut(s) 331
BstC8I GCNNGC 2 cut(s) 332, 519
BstDSI CCRYGG 1 cut(s) 82
BstF5I GGATG 3 cut(s) 148, 152, 302
BstHHI GCGC 1 cut(s) 330
BstKTI GATC 2 cut(s) 420, 556
BstMAI GTCTC 1 cut(s) 272
BstMBI GATC 2 cut(s) 417, 553
BstMWI GCNNNNNNNGC 2 cut(s) 103, 331
BstNSI RCATGY 3 cut(s) 101, 334, 396
BstSCI CCNGG 1 cut(s) 534
BstV1I GCAGC 1 cut(s) 337
BstV2I GAAGAC 1 cut(s) 590
BsuRI GGCC 2 cut(s) 9, 517
BtgI CCRYGG 1 cut(s) 82
BtsCI GGATG 3 cut(s) 148, 152, 302
Cac8I GCNNGC 2 cut(s) 332, 519
CciI TCATGA 1 cut(s) 112
CfoI GCGC 1 cut(s) 330
Cfr10I RCCGGY 1 cut(s) 122
Cfr13I GGNCC 1 cut(s) 7
CseI GACGC 1 cut(s) 67
Csp6I GTAC 1 cut(s) 40
CspAI ACCGGT 1 cut(s) 122
CviAII CATG 7 cut(s) 29, 98, 113, 322, 331, 393, 557
CviJI RGCY 9 cut(s) 9, 45, 106, 175, 190, 230, 502, 517, 534
CviKI_1 RGCY 9 cut(s) 9, 45, 106, 175, 190, 230, 502, 517, 534
CviQI GTAC 1 cut(s) 40
DpnI GATC 2 cut(s) 419, 555
DpnII GATC 2 cut(s) 417, 553
DraI TTTAAA 1 cut(s) 355
Eco31I GGTCTC 1 cut(s) 272
Eco57I CTGAAG 1 cut(s) 434
EcoO109I RGGNCCY 1 cut(s) 7
FaeI CATG 7 cut(s) 32, 101, 116, 325, 334, 396, 560
FatI CATG 7 cut(s) 28, 97, 112, 321, 330, 392, 556
FauI CCCGC 1 cut(s) 503
Fnu4HI GCNGC 2 cut(s) 231, 326
FokI GGATG 3 cut(s) 155, 159, 309
Fsp4HI GCNGC 2 cut(s) 231, 326
FspBI CTAG 1 cut(s) 449
GlaI GCGC 1 cut(s) 329
GluI GCNGC 2 cut(s) 231, 326
GsuI CTGGAG 1 cut(s) 179
HaeIII GGCC 2 cut(s) 9, 517
HapII CCGG 3 cut(s) 103, 123, 535
HgaI GACGC 1 cut(s) 67
HhaI GCGC 1 cut(s) 330
Hin1I GRCGYC 1 cut(s) 78
Hin1II CATG 7 cut(s) 32, 101, 116, 325, 334, 396, 560
Hin6I GCGC 1 cut(s) 328
HinP1I GCGC 1 cut(s) 328
HindIII AAGCTT 1 cut(s) 500
HinfI GANTC 1 cut(s) 156
HpaII CCGG 3 cut(s) 103, 123, 535
HphI GGTGA 2 cut(s) 550, 590
Hpy188I TCNGA 2 cut(s) 390, 414
Hpy188III TCNNGA 1 cut(s) 113
Hpy99I CGWCG 1 cut(s) 372
HpyAV CCTTC 2 cut(s) 492, 542
HpyCH4V TGCA 3 cut(s) 4, 325, 460
HpyF10VI GCNNNNNNNGC 2 cut(s) 103, 331
Hsp92I GRCGYC 1 cut(s) 78
Hsp92II CATG 7 cut(s) 32, 101, 116, 325, 334, 396, 560
HspAI GCGC 1 cut(s) 328
Kzo9I GATC 2 cut(s) 417, 553
LpnPI CCDG 7 cut(s) 116, 136, 204, 209, 520, 531, 548
Lsp1109I GCAGC 1 cut(s) 337
LweI GCATC 1 cut(s) 202
MaeI CTAG 1 cut(s) 449
MaeIII GTNAC 1 cut(s) 125
MalI GATC 2 cut(s) 419, 555
MboI GATC 2 cut(s) 417, 553
MboII GAAGA 2 cut(s) 427, 595
MfeI CAATTG 1 cut(s) 563
MluCI AATT 6 cut(s) 60, 265, 350, 430, 563, 607
MmeI TCCRAC 2 cut(s) 255, 413
MnlI CCTC 3 cut(s) 20, 67, 247
MseI TTAA 6 cut(s) 36, 264, 354, 402, 543, 591
MspA1I CMGCKG 1 cut(s) 510
MspI CCGG 3 cut(s) 103, 123, 535
MspR9I CCNGG 1 cut(s) 536
MunI CAATTG 1 cut(s) 563
MwoI GCNNNNNNNGC 2 cut(s) 103, 331
NciI CCSGG 1 cut(s) 536
NdeII GATC 2 cut(s) 417, 553
NlaIII CATG 7 cut(s) 32, 101, 116, 325, 334, 396, 560
NlaIV GGNNCC 1 cut(s) 121
NspI RCATGY 3 cut(s) 101, 334, 396
PaeI GCATGC 1 cut(s) 334
PagI TCATGA 1 cut(s) 112
PciI ACATGT 1 cut(s) 392
PfeI GAWTC 1 cut(s) 156
PflMI CCANNNNNTGG 1 cut(s) 513
PinAI ACCGGT 1 cut(s) 122
PkrI GCNGC 2 cut(s) 232, 327
PscI ACATGT 1 cut(s) 392
PspN4I GGNNCC 1 cut(s) 121
PspPI GGNCC 1 cut(s) 7
RsaI GTAC 1 cut(s) 41
RsaNI GTAC 1 cut(s) 40
SaqAI TTAA 6 cut(s) 36, 264, 354, 402, 543, 591
SatI GCNGC 2 cut(s) 231, 326
Sau3AI GATC 2 cut(s) 417, 553
Sau96I GGNCC 1 cut(s) 7
ScrFI CCNGG 1 cut(s) 536
SetI ASST 7 cut(s) 177, 192, 340, 450, 504, 580, 590
SfaNI GCATC 1 cut(s) 202
SphI GCATGC 1 cut(s) 334
Sse9I AATT 6 cut(s) 60, 265, 350, 430, 563, 607
SsiI CCGC 2 cut(s) 231, 510
SspMI CTAG 1 cut(s) 449
StyD4I CCNGG 1 cut(s) 534
TaqI TCGA 1 cut(s) 423
TaqII GACCGA 1 cut(s) 296
TasI AATT 6 cut(s) 60, 265, 350, 430, 563, 607
TauI GCSGC 1 cut(s) 233
TfiI GAWTC 1 cut(s) 156
Tru1I TTAA 6 cut(s) 36, 264, 354, 402, 543, 591
Tru9I TTAA 6 cut(s) 36, 264, 354, 402, 543, 591
TseI GCWGC 1 cut(s) 325
TspDTI ATGAA 1 cut(s) 101
TspGWI ACGGA 1 cut(s) 71
Van91I CCANNNNNTGG 1 cut(s) 513
XapI RAATTY 2 cut(s) 350, 430
XceI RCATGY 3 cut(s) 101, 334, 396
XspI CTAG 1 cut(s) 449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.