Rh6AG195600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0003488
Physical Location & Seq
Forward (+)
35154418 .. 35166253
11836 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG195600.1

Sequence Viewer

Length: 591 bp
ATGCGCATATCTCGGCTTAGGGAGGGCAATATAGAAGCACACAAGGTGGAGGAGGTGAAGCATGCGAAGAGTAAAGGCCGAAAACATAAGCATAAGAGCCTGAAACGAAAATTTGGTGCATGTGCAAATGAAGGTGTGATTTCAGGGAAGAATGGTGATCTATTAAGGTTCCTATTGCGGAAAAACTTTGTAGGCGAAGACAAATGGAGACGATGGGCTGTGCGGCTATTTCGTGATGGAGCTAGCAGCTTTTGGTTGAGGGAAGGATGGGTCAGATTCTATGAGGACAACAATCTTGGCAACAATGAGTTAGTTGAGTTCATTTTTGACGAGAAAACCGGTAATGCTAATGTTACCATTGTTGTTCCGGTGGATGAAGTAGTTAACTATGCTTTGAACACTGATGATGAGATGGGTTGTGACATTTATGATAATCAAGCCATGCAACTCATGCGGCCTGTTGTGGTTGTTTTCATTGTATTTCGAGGGTTATTGTATATTTATCATATGCAGAACATTTCGTCGTTTGTTGTCAAGCGTTACCTGAATGTGGAGTACTATAATGTTACCATTATAGACCCTAAACTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

196

Amino Acids

22.95

Weight (kDa)

9.28

Isoelectric Point (pI)

38.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 70 - 120 3.2e-06 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 5
AciI CCGC 3 cut(s) 178, 223, 454
AcsI RAATTY 1 cut(s) 110
AdeI CACNNNGTG 1 cut(s) 46
AfaI GTAC 1 cut(s) 557
AfiI CCNNNNNNNGG 1 cut(s) 550
AgeI ACCGGT 1 cut(s) 338
AgsI TTSAA 1 cut(s) 397
AluBI AGCT 2 cut(s) 242, 249
AluI AGCT 2 cut(s) 242, 249
Alw26I GTCTC 1 cut(s) 202
AoxI GGCC 2 cut(s) 76, 455
ApeKI GCWGC 1 cut(s) 246
ApoI RAATTY 1 cut(s) 110
AsiGI ACCGGT 1 cut(s) 338
AspLEI GCGC 1 cut(s) 6
AsuHPI GGTGA 2 cut(s) 67, 167
AsuNHI GCTAGC 1 cut(s) 242
BbsI GAAGAC 1 cut(s) 204
BbvI GCAGC 1 cut(s) 258
BccI CCATC 4 cut(s) 207, 230, 261, 406
BcoDI GTCTC 1 cut(s) 202
BfaI CTAG 1 cut(s) 243
BisI GCNGC 3 cut(s) 224, 247, 455
BlsI GCNGC 3 cut(s) 225, 248, 456
BmcAI AGTACT 1 cut(s) 557
BmiI GGNNCC 1 cut(s) 170
BmtI GCTAGC 1 cut(s) 246
BpiI GAAGAC 1 cut(s) 204
Bpu10I CCTNAGC 1 cut(s) 17
BsaWI WCCGGW 2 cut(s) 338, 367
Bsc4I CCNNNNNNNGG 1 cut(s) 550
Bse118I RCCGGY 1 cut(s) 338
BseGI GGATG 2 cut(s) 272, 379
BseLI CCNNNNNNNGG 1 cut(s) 550
BseRI GAGGAG 1 cut(s) 65
BseXI GCAGC 1 cut(s) 258
BshFI GGCC 2 cut(s) 78, 457
BshTI ACCGGT 1 cut(s) 338
BsiSI CCGG 2 cut(s) 339, 368
BslI CCNNNNNNNGG 1 cut(s) 550
BsmAI GTCTC 1 cut(s) 202
BsmBI CGTCTC 1 cut(s) 202
BsnI GGCC 2 cut(s) 78, 457
Bsp143I GATC 1 cut(s) 157
BspACI CCGC 3 cut(s) 178, 223, 454
BspANI GGCC 2 cut(s) 78, 457
BspLI GGNNCC 1 cut(s) 170
BspOI GCTAGC 1 cut(s) 246
BsrFI RCCGGY 1 cut(s) 338
BssAI RCCGGY 1 cut(s) 338
BssMI GATC 1 cut(s) 157
Bst6I CTCTTC 1 cut(s) 62
BstAPI GCANNNNNTGC 1 cut(s) 451
BstC8I GCNNGC 2 cut(s) 63, 244
BstDEI CTNAG 1 cut(s) 17
BstF5I GGATG 2 cut(s) 272, 379
BstHHI GCGC 1 cut(s) 6
BstKTI GATC 1 cut(s) 160
BstMAI GTCTC 1 cut(s) 202
BstMBI GATC 1 cut(s) 157
BstMWI GCNNNNNNNGC 1 cut(s) 451
BstNSI RCATGY 2 cut(s) 65, 123
BstV1I GCAGC 1 cut(s) 258
BstV2I GAAGAC 1 cut(s) 204
BsuRI GGCC 2 cut(s) 78, 457
BtsCI GGATG 2 cut(s) 272, 379
BtsIMutI CAGTG 1 cut(s) 399
Cac8I GCNNGC 2 cut(s) 63, 244
CfoI GCGC 1 cut(s) 6
Cfr10I RCCGGY 1 cut(s) 338
Csp6I GTAC 1 cut(s) 556
CspAI ACCGGT 1 cut(s) 338
CviAII CATG 4 cut(s) 62, 120, 442, 451
CviJI RGCY 9 cut(s) 16, 78, 99, 218, 226, 242, 249, 440, 457
CviKI_1 RGCY 9 cut(s) 16, 78, 99, 218, 226, 242, 249, 440, 457
CviQI GTAC 1 cut(s) 556
DdeI CTNAG 1 cut(s) 17
DpnI GATC 1 cut(s) 159
DpnII GATC 1 cut(s) 157
DraIII CACNNNGTG 1 cut(s) 46
Eam1104I CTCTTC 1 cut(s) 62
EarI CTCTTC 1 cut(s) 62
Esp3I CGTCTC 1 cut(s) 202
FaeI CATG 4 cut(s) 65, 123, 445, 454
FatI CATG 4 cut(s) 61, 119, 441, 450
FauNDI CATATG 1 cut(s) 507
Fnu4HI GCNGC 3 cut(s) 224, 247, 455
FokI GGATG 2 cut(s) 279, 386
Fsp4HI GCNGC 3 cut(s) 224, 247, 455
FspAI RTGCGCAY 1 cut(s) 5
FspBI CTAG 1 cut(s) 243
FspI TGCGCA 1 cut(s) 5
GlaI GCGC 1 cut(s) 5
GluI GCNGC 3 cut(s) 224, 247, 455
HaeIII GGCC 2 cut(s) 78, 457
HapII CCGG 2 cut(s) 339, 368
HhaI GCGC 1 cut(s) 6
Hin1II CATG 4 cut(s) 65, 123, 445, 454
Hin6I GCGC 1 cut(s) 4
HinP1I GCGC 1 cut(s) 4
HincII GTYRAC 1 cut(s) 385
HindII GTYRAC 1 cut(s) 385
HinfI GANTC 1 cut(s) 276
HpaI GTTAAC 1 cut(s) 385
HpaII CCGG 2 cut(s) 339, 368
HphI GGTGA 2 cut(s) 67, 167
Hpy166II GTNNAC 1 cut(s) 385
Hpy188I TCNGA 2 cut(s) 275, 590
Hpy188III TCNNGA 1 cut(s) 233
Hpy8I GTNNAC 1 cut(s) 385
Hpy99I CGWCG 1 cut(s) 526
HpyAV CCTTC 2 cut(s) 125, 257
HpyCH4V TGCA 4 cut(s) 119, 125, 445, 511
HpyF10VI GCNNNNNNNGC 1 cut(s) 451
HpyF3I CTNAG 1 cut(s) 17
Hsp92II CATG 4 cut(s) 65, 123, 445, 454
HspAI GCGC 1 cut(s) 4
KspAI GTTAAC 1 cut(s) 385
Kzo9I GATC 1 cut(s) 157
LmnI GCTCC 1 cut(s) 239
LpnPI CCDG 6 cut(s) 113, 129, 352, 381, 471, 557
Lsp1109I GCAGC 1 cut(s) 258
MaeI CTAG 1 cut(s) 243
MaeIII GTNAC 4 cut(s) 352, 419, 539, 565
MalI GATC 1 cut(s) 159
MboI GATC 1 cut(s) 157
MboII GAAGA 3 cut(s) 79, 160, 209
MluCI AATT 1 cut(s) 110
MnlI CCTC 6 cut(s) 16, 43, 46, 252, 277, 479
MseI TTAA 2 cut(s) 164, 384
MspI CCGG 2 cut(s) 339, 368
MwoI GCNNNNNNNGC 1 cut(s) 451
NdeI CATATG 1 cut(s) 507
NdeII GATC 1 cut(s) 157
NheI GCTAGC 1 cut(s) 242
NlaIII CATG 4 cut(s) 65, 123, 445, 454
NlaIV GGNNCC 1 cut(s) 170
NmuCI GTSAC 1 cut(s) 419
NsbI TGCGCA 1 cut(s) 5
NspI RCATGY 2 cut(s) 65, 123
PaeI GCATGC 1 cut(s) 65
PfeI GAWTC 1 cut(s) 276
PinAI ACCGGT 1 cut(s) 338
PkrI GCNGC 3 cut(s) 225, 248, 456
PspN4I GGNNCC 1 cut(s) 170
RsaI GTAC 1 cut(s) 557
RsaNI GTAC 1 cut(s) 556
SaqAI TTAA 2 cut(s) 164, 384
SatI GCNGC 3 cut(s) 224, 247, 455
Sau3AI GATC 1 cut(s) 157
ScaI AGTACT 1 cut(s) 557
SetI ASST 7 cut(s) 48, 57, 136, 170, 244, 251, 546
SphI GCATGC 1 cut(s) 65
Sse9I AATT 1 cut(s) 110
SsiI CCGC 3 cut(s) 178, 223, 454
SspMI CTAG 1 cut(s) 243
TaqI TCGA 1 cut(s) 484
TasI AATT 1 cut(s) 110
TatI WGTACW 1 cut(s) 555
TauI GCSGC 2 cut(s) 226, 457
TfiI GAWTC 1 cut(s) 276
Tru1I TTAA 2 cut(s) 164, 384
Tru9I TTAA 2 cut(s) 164, 384
TscAI CASTG 1 cut(s) 406
TseFI GTSAC 1 cut(s) 419
TseI GCWGC 1 cut(s) 246
Tsp45I GTSAC 1 cut(s) 419
TspDTI ATGAA 4 cut(s) 144, 310, 390, 463
TspRI CASTG 1 cut(s) 406
XapI RAATTY 1 cut(s) 110
XceI RCATGY 2 cut(s) 65, 123
XspI CTAG 1 cut(s) 243
ZrmI AGTACT 1 cut(s) 557
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.