RLG00000008289

AtPRD1,PRD1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0002682
Physical Location & Seq
Forward (+)
28355634 .. 28358802
3169 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008289

Sequence Viewer

Length: 1344 bp
ATGGAGGAGGAGGATGATAGAGTTTTCGATTCCGGAGTTCTCTTAAACTGCCAGCGTGGTAATCCTTTTGCTCACATTAGAGACCAAAATGGCCTCATTACAAATCTTGTTTCAGGTCTTCAATTACCAAGTGAAGAGATTCGGGGAGAAACCATGTTTGTTCTGTACAAAGTATCTGCTCTCCACTATGCATCAGAGGATGCTGATGGATCTGATCTTTTATTTTCCTTTTGCCCCAAGTTAGTGCGCCTGTCTCTGGAGGCCCTCATGAAGACCCAAAGTGATGACGTCCGCTTGAATTGTATAGCTCTTTTGACGGTCTTGGCTCGGAGAGGGCTTTTTGGGACTGCATATGCCCTTGATTTAAACAGCATGAGTTCATCTGAAGGAGATAGCTTTGGGCAAGCAACAGAAGATGGAAAAGATATGCATCCTCTGAACATATTATTTACTGAGATCAGCACACTAGATTTACTCTTTCACTATATCTCCTTTGAAGGCACTTCATCCAGACAAATCCAAGTGCTAGTTGAAGAAGGCATTGCAGATTATGTGTTTGAAAGACTGAGATTGTCAGAATGCAAGGATCCGGCTGTCAAATCTTGCCTTCAGGTACTTGATATTTTATCAAAAGCTGAGCAAGCTTTCAAACAGAGGCTTCTTGTTGGGTTTGCAATTCTGGTTCCAGTACTACATTATGTAGCTGAAGTTCCTTTCCATCCGGTTCAAAATCAAACTCTGAAGCTTATTTTGAACTGCATTTCTGATTGCCCCGGAATGCTATCCTCCTCTCATATAGAGGAACTAGCTCCTGTTTTAACAAAGATGCTTAAACAACATGCTGATGGGGATATGGGCATGCTTGAGGAGACATTTATAATGACCTGTTTAGTCCTTGTAGCTATCATAAGAACTCCATCTTTTCATGTGAGTTTGAATCTAGCACCATCAATTAAAGAAGCATTAGAATATGCTGTCTTACCTTGTCTAAGTACATCCATCAAACATCCGGGTCAACTTCTGCATTCCTTGTACCTACTTAAAGAGGCATACATGTATAGTCGTGAAGAAAATTCTACTGACTCCTCTAAAATAGAACTACGAAATTTGATTTTGAGTTTGTGCACAAAACATTTGTTGCCTTGGCTGGTAACCACCTTAAATGAAATGGAAGAGGAAACTATCCTTGGAGTACTGGAAACTTTTCATTCGATACTGCTTCAGGATTCTGATAACCAAGCTAGAGAATTTTCAATGACGCTACTTTCGAGCAATTGGTTCACTTTGTCATTTGGATGTCTAGGCTTATTCCCCAATGGTTCAAAATATTTAACAAATTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

448

Amino Acids

50.03

Weight (kDa)

4.89

Isoelectric Point (pI)

56.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 878
AatII GACGTC 1 cut(s) 291
AccIII TCCGGA 1 cut(s) 32
AciI CCGC 1 cut(s) 292
AclWI GGATC 3 cut(s) 217, 581, 594
AcsI RAATTY 3 cut(s) 1072, 1105, 1247
AcuI CTGAAG 5 cut(s) 405, 593, 726, 761, 1205
AcyI GRCGYC 1 cut(s) 288
AfaI GTAC 6 cut(s) 167, 615, 690, 994, 1034, 1194
AfiI CCNNNNNNNGG 1 cut(s) 256
AflIII ACRYGT 1 cut(s) 1053
AjuI GAANNNNNNNTTGG 2 cut(s) 1170, 1202
AluBI AGCT 9 cut(s) 308, 396, 635, 644, 704, 745, 809, 902, 1241
AluI AGCT 9 cut(s) 308, 396, 635, 644, 704, 745, 809, 902, 1241
Alw21I GWGCWC 1 cut(s) 1127
Alw26I GTCTC 3 cut(s) 75, 258, 863
Alw44I GTGCAC 1 cut(s) 1123
AlwI GGATC 3 cut(s) 217, 581, 594
Aor13HI TCCGGA 1 cut(s) 32
AoxI GGCC 2 cut(s) 91, 261
ApaLI GTGCAC 1 cut(s) 1123
ApoI RAATTY 3 cut(s) 1072, 1105, 1247
ArsI GACNNNNNNTTYG 2 cut(s) 1249, 1281
Asp700I GAANNNNTTC 2 cut(s) 138, 1203
AspLEI GCGC 1 cut(s) 249
AspS9I GGNCC 1 cut(s) 262
AsuC2I CCSGG 2 cut(s) 774, 1011
BaeGI GKGCMC 1 cut(s) 1127
BamHI GGATCC 1 cut(s) 586
BbsI GAAGAC 2 cut(s) 110, 278
Bbv12I GWGCWC 1 cut(s) 1127
BccI CCATC 7 cut(s) 200, 410, 726, 839, 925, 955, 1007
BcnI CCSGG 2 cut(s) 774, 1011
BcoDI GTCTC 3 cut(s) 75, 258, 863
BfaI CTAG 6 cut(s) 467, 527, 806, 941, 1242, 1301
BlpI GCTNAGC 1 cut(s) 636
BmcAI AGTACT 2 cut(s) 690, 1194
Bme1390I CCNGG 2 cut(s) 774, 1011
BmgT120I GGNCC 1 cut(s) 262
BmiI GGNNCC 2 cut(s) 588, 684
BmrFI CCNGG 2 cut(s) 774, 1011
BmsI GCATC 4 cut(s) 190, 200, 439, 816
BpiI GAAGAC 2 cut(s) 110, 278
BpmI CTGGAG 1 cut(s) 278
Bpu1102I GCTNAGC 1 cut(s) 636
BpuEI CTTGAG 1 cut(s) 884
BpuMI CCSGG 2 cut(s) 774, 1011
BsaBI GATNNNNATC 1 cut(s) 429
BsaHI GRCGYC 1 cut(s) 288
BsaI GGTCTC 1 cut(s) 75
BsaJI CCNNGG 3 cut(s) 772, 1142, 1186
BsaWI WCCGGW 2 cut(s) 32, 721
BsaXI ACNNNNNCTCC 2 cut(s) 473, 503
Bsc4I CCNNNNNNNGG 1 cut(s) 256
Bse1I ACTGG 2 cut(s) 686, 1200
Bse3DI GCAATG 1 cut(s) 540
Bse8I GATNNNNATC 1 cut(s) 429
BseAI TCCGGA 1 cut(s) 32
BseDI CCNNGG 3 cut(s) 772, 1142, 1186
BseGI GGATG 8 cut(s) 19, 205, 430, 506, 718, 995, 1006, 1301
BseJI GATNNNNATC 1 cut(s) 429
BseLI CCNNNNNNNGG 1 cut(s) 256
BseMI GCAATG 1 cut(s) 540
BseMII CTCAG 3 cut(s) 444, 557, 627
BseNI ACTGG 2 cut(s) 686, 1200
BseRI GAGGAG 5 cut(s) 20, 23, 778, 881, 1075
BseSI GKGCMC 1 cut(s) 1127
BshFI GGCC 2 cut(s) 93, 263
BsiHKAI GWGCWC 1 cut(s) 1127
BsiSI CCGG 5 cut(s) 33, 590, 722, 774, 1010
BslFI GGGAC 1 cut(s) 358
BslI CCNNNNNNNGG 1 cut(s) 256
BsmAI GTCTC 3 cut(s) 75, 258, 863
BsmFI GGGAC 1 cut(s) 358
BsmI GAATGC 3 cut(s) 584, 783, 1024
BsnI GGCC 2 cut(s) 93, 263
Bso31I GGTCTC 1 cut(s) 75
Bsp1286I GDGCHC 1 cut(s) 1127
Bsp13I TCCGGA 1 cut(s) 32
Bsp1407I TGTACA 1 cut(s) 165
Bsp143I GATC 4 cut(s) 209, 214, 456, 586
Bsp1720I GCTNAGC 1 cut(s) 636
BspACI CCGC 1 cut(s) 292
BspANI GGCC 2 cut(s) 93, 263
BspCNI CTCAG 3 cut(s) 445, 558, 628
BspEI TCCGGA 1 cut(s) 32
BspHI TCATGA 1 cut(s) 267
BspLI GGNNCC 2 cut(s) 588, 684
BspPI GGATC 3 cut(s) 217, 581, 594
BspTNI GGTCTC 1 cut(s) 75
BsrDI GCAATG 1 cut(s) 540
BsrGI TGTACA 1 cut(s) 165
BsrI ACTGG 2 cut(s) 686, 1200
BssECI CCNNGG 3 cut(s) 772, 1142, 1186
BssMI GATC 4 cut(s) 209, 214, 456, 586
BssNI GRCGYC 1 cut(s) 288
BssT1I CCWWGG 2 cut(s) 1142, 1186
Bst4CI ACNGT 1 cut(s) 319
Bst6I CTCTTC 2 cut(s) 129, 1167
BstACI GRCGYC 1 cut(s) 288
BstAUI TGTACA 1 cut(s) 165
BstC8I GCNNGC 4 cut(s) 53, 405, 642, 860
BstDEI CTNAG 4 cut(s) 453, 566, 636, 989
BstEII GGTNACC 1 cut(s) 1150
BstF5I GGATG 8 cut(s) 19, 205, 430, 506, 718, 995, 1006, 1301
BstHHI GCGC 1 cut(s) 249
BstKTI GATC 4 cut(s) 212, 217, 459, 589
BstMAI GTCTC 3 cut(s) 75, 258, 863
BstMBI GATC 4 cut(s) 209, 214, 456, 586
BstMWI GCNNNNNNNGC 1 cut(s) 641
BstNSI RCATGY 3 cut(s) 842, 862, 1057
BstPI GGTNACC 1 cut(s) 1150
BstSCI CCNGG 2 cut(s) 772, 1009
BstSLI GKGCMC 1 cut(s) 1127
BstV2I GAAGAC 2 cut(s) 110, 278
BstX2I RGATCY 2 cut(s) 209, 586
BstYI RGATCY 2 cut(s) 209, 586
BsuRI GGCC 2 cut(s) 93, 263
BtsCI GGATG 8 cut(s) 19, 205, 430, 506, 718, 995, 1006, 1301
Cac8I GCNNGC 4 cut(s) 53, 405, 642, 860
CciI TCATGA 1 cut(s) 267
CfoI GCGC 1 cut(s) 249
Cfr13I GGNCC 1 cut(s) 262
CseI GACGC 1 cut(s) 1267
Csp6I GTAC 6 cut(s) 166, 614, 689, 993, 1033, 1193
CviAII CATG 7 cut(s) 154, 268, 373, 839, 859, 926, 1054
CviQI GTAC 6 cut(s) 166, 614, 689, 993, 1033, 1193
DdeI CTNAG 4 cut(s) 453, 566, 636, 989
DpnI GATC 4 cut(s) 211, 216, 458, 588
DpnII GATC 4 cut(s) 209, 214, 456, 586
DraI TTTAAA 1 cut(s) 366
Eam1104I CTCTTC 2 cut(s) 129, 1167
EarI CTCTTC 2 cut(s) 129, 1167
Eco130I CCWWGG 2 cut(s) 1142, 1186
Eco31I GGTCTC 1 cut(s) 75
Eco57I CTGAAG 5 cut(s) 405, 593, 726, 761, 1205
Eco91I GGTNACC 1 cut(s) 1150
EcoO109I RGGNCCY 1 cut(s) 262
EcoO65I GGTNACC 1 cut(s) 1150
EcoT14I CCWWGG 2 cut(s) 1142, 1186
EcoT22I ATGCAT 2 cut(s) 193, 432
ErhI CCWWGG 2 cut(s) 1142, 1186
FaeI CATG 7 cut(s) 157, 271, 376, 842, 862, 929, 1057
FaqI GGGAC 1 cut(s) 358
FatI CATG 7 cut(s) 153, 267, 372, 838, 858, 925, 1053
FauNDI CATATG 1 cut(s) 352
FokI GGATG 8 cut(s) 26, 212, 417, 493, 705, 982, 993, 1308
FspBI CTAG 6 cut(s) 467, 527, 806, 941, 1242, 1301
GlaI GCGC 1 cut(s) 248
GsuI CTGGAG 1 cut(s) 278
HaeIII GGCC 2 cut(s) 93, 263
HapII CCGG 5 cut(s) 33, 590, 722, 774, 1010
HgaI GACGC 1 cut(s) 1267
HhaI GCGC 1 cut(s) 249
Hin1I GRCGYC 1 cut(s) 288
Hin1II CATG 7 cut(s) 157, 271, 376, 842, 862, 929, 1057
Hin6I GCGC 1 cut(s) 247
HinP1I GCGC 1 cut(s) 247
HincII GTYRAC 1 cut(s) 1016
HindII GTYRAC 1 cut(s) 1016
HindIII AAGCTT 2 cut(s) 642, 743
HinfI GANTC 5 cut(s) 29, 139, 937, 1082, 1226
HpaII CCGG 5 cut(s) 33, 590, 722, 774, 1010
Hpy166II GTNNAC 3 cut(s) 1016, 1125, 1281
Hpy188I TCNGA 9 cut(s) 196, 214, 330, 385, 438, 577, 741, 766, 1231
Hpy188III TCNNGA 6 cut(s) 33, 257, 268, 510, 1064, 1223
Hpy8I GTNNAC 3 cut(s) 1016, 1125, 1281
HpyAV CCTTC 4 cut(s) 380, 491, 530, 617
HpyCH4III ACNGT 1 cut(s) 319
HpyCH4IV ACGT 1 cut(s) 288
HpyCH4V TGCA 9 cut(s) 191, 350, 430, 545, 582, 674, 759, 1024, 1125
HpyF10VI GCNNNNNNNGC 1 cut(s) 641
HpyF3I CTNAG 4 cut(s) 453, 566, 636, 989
HpySE526I ACGT 1 cut(s) 288
Hsp92I GRCGYC 1 cut(s) 288
Hsp92II CATG 7 cut(s) 157, 271, 376, 842, 862, 929, 1057
HspAI GCGC 1 cut(s) 247
Kpn2I TCCGGA 1 cut(s) 32
Kzo9I GATC 4 cut(s) 209, 214, 456, 586
LmnI GCTCC 1 cut(s) 814
LweI GCATC 4 cut(s) 190, 200, 439, 816
MaeI CTAG 6 cut(s) 467, 527, 806, 941, 1242, 1301
MaeII ACGT 1 cut(s) 288
MaeIII GTNAC 1 cut(s) 1150
MalI GATC 4 cut(s) 211, 216, 458, 588
MboI GATC 4 cut(s) 209, 214, 456, 586
MboII GAAGA 7 cut(s) 110, 146, 283, 425, 545, 1079, 1184
MfeI CAATTG 1 cut(s) 1273
MflI RGATCY 2 cut(s) 209, 586
MhlI GDGCHC 1 cut(s) 1127
MluCI AATT 9 cut(s) 122, 298, 675, 951, 1072, 1105, 1247, 1273, 1336
MlyI GAGTC 1 cut(s) 1076
Mph1103I ATGCAT 2 cut(s) 193, 432
MroI TCCGGA 1 cut(s) 32
MroXI GAANNNNTTC 2 cut(s) 138, 1203
MseI TTAA 8 cut(s) 44, 365, 818, 831, 954, 1041, 1160, 1331
MslI CAYNNNNRTG 2 cut(s) 843, 1294
MspI CCGG 5 cut(s) 33, 590, 722, 774, 1010
MspR9I CCNGG 2 cut(s) 774, 1011
MunI CAATTG 1 cut(s) 1273
Mva1269I GAATGC 3 cut(s) 584, 783, 1024
MwoI GCNNNNNNNGC 1 cut(s) 641
NciI CCSGG 2 cut(s) 774, 1011
NdeI CATATG 1 cut(s) 352
NdeII GATC 4 cut(s) 209, 214, 456, 586
NlaIII CATG 7 cut(s) 157, 271, 376, 842, 862, 929, 1057
NlaIV GGNNCC 2 cut(s) 588, 684
NsiI ATGCAT 2 cut(s) 193, 432
NspI RCATGY 3 cut(s) 842, 862, 1057
PaeI GCATGC 1 cut(s) 862
PagI TCATGA 1 cut(s) 267
PciI ACATGT 1 cut(s) 1053
PcsI WCGNNNNNNNCGW 1 cut(s) 1265
PctI GAATGC 3 cut(s) 584, 783, 1024
PdmI GAANNNNTTC 2 cut(s) 138, 1203
PfeI GAWTC 4 cut(s) 29, 139, 937, 1226
PleI GAGTC 1 cut(s) 1076
PpsI GAGTC 1 cut(s) 1076
PscI ACATGT 1 cut(s) 1053
PsiI TTATAA 1 cut(s) 878
PspEI GGTNACC 1 cut(s) 1150
PspN4I GGNNCC 2 cut(s) 588, 684
PspPI GGNCC 1 cut(s) 262
PsrI GAACNNNNNNTAC 2 cut(s) 693, 725
PsuI RGATCY 2 cut(s) 209, 586
RsaI GTAC 6 cut(s) 167, 615, 690, 994, 1034, 1194
RsaNI GTAC 6 cut(s) 166, 614, 689, 993, 1033, 1193
RseI CAYNNNNRTG 2 cut(s) 843, 1294
SaqAI TTAA 8 cut(s) 44, 365, 818, 831, 954, 1041, 1160, 1331
Sau3AI GATC 4 cut(s) 209, 214, 456, 586
Sau96I GGNCC 1 cut(s) 262
ScaI AGTACT 2 cut(s) 690, 1194
SchI GAGTC 1 cut(s) 1076
ScrFI CCNGG 2 cut(s) 774, 1011
SduI GDGCHC 1 cut(s) 1127
SfaNI GCATC 4 cut(s) 190, 200, 439, 816
SmiMI CAYNNNNRTG 2 cut(s) 843, 1294
SmlI CTYRAG 1 cut(s) 863
SmoI CTYRAG 1 cut(s) 863
SphI GCATGC 1 cut(s) 862
Sse9I AATT 9 cut(s) 122, 298, 675, 951, 1072, 1105, 1247, 1273, 1336
SsiI CCGC 1 cut(s) 292
SspI AATATT 1 cut(s) 1328
SspMI CTAG 6 cut(s) 467, 527, 806, 941, 1242, 1301
StyD4I CCNGG 2 cut(s) 772, 1009
StyI CCWWGG 2 cut(s) 1142, 1186
TaaI ACNGT 1 cut(s) 319
TaiI ACGT 1 cut(s) 291
TaqI TCGA 3 cut(s) 27, 1211, 1268
TasI AATT 9 cut(s) 122, 298, 675, 951, 1072, 1105, 1247, 1273, 1336
TatI WGTACW 4 cut(s) 165, 688, 992, 1192
TfiI GAWTC 4 cut(s) 29, 139, 937, 1226
Tru1I TTAA 8 cut(s) 44, 365, 818, 831, 954, 1041, 1160, 1331
Tru9I TTAA 8 cut(s) 44, 365, 818, 831, 954, 1041, 1160, 1331
TspDTI ATGAA 6 cut(s) 284, 369, 495, 914, 1179, 1196
VneI GTGCAC 1 cut(s) 1123
XapI RAATTY 3 cut(s) 1072, 1105, 1247
XceI RCATGY 3 cut(s) 842, 862, 1057
XmnI GAANNNNTTC 2 cut(s) 138, 1203
XspI CTAG 6 cut(s) 467, 527, 806, 941, 1242, 1301
ZraI GACGTC 1 cut(s) 289
ZrmI AGTACT 2 cut(s) 690, 1194
Zsp2I ATGCAT 2 cut(s) 193, 432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.