RLG00000008411

DNA topoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0007552
Physical Location & Seq
Forward (+)
30223420 .. 30225984
2565 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008411

Sequence Viewer

Length: 549 bp
ATGCTGAGGGGAGTGCTTCCCACCTCTGGACCTCCAGTACTTAGAACTTCTAGAATTTCCAAATTTGGGTATCTAGCACTTAGTACTTCCTTGAAGAGGCAAAGTGACAAGGAGAAATTTGAGAAAGCAAGGACGTTGAAGGACTACATAGGAGCTATCAGGGCTGCATACACTCGGGATATTGCAAGTGAGGATGGTCAAAAGCGGCAGCTAGCAGTGGCTACCTATCTCATGGATAAACTTGGTCTAAGGGCTGGAACTGAGAAGGTTGATGAGGATGATGATGAAACTGATATTGTCAGTTGCCGCAGATTAAATCATCAGCGTGATATCGAAGAGCCTCATAGTGCACAAATGTCTAGGATAATAGAGAAGATTGGTGAGCTTCAGGTTGATATCATCTCTAATCATAAGCGCAATATCACAGGATCCTATAGTGCACAATTTGATAGCTTAATGGAGAAGATCGGTGAGCTTCAGGTATTTCCAAAGTCCCTCTTGAGAAAGTTTGCTTGGGCAATGGATGTCGGCCCTGACTTCAGATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Topo_C_assoc Topoisom_I Topoisom_I_N

Protein Analysis

183

Amino Acids

20.7

Weight (kDa)

8.94

Isoelectric Point (pI)

46.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Topoisom_I PF01028 29 - 97 1.4e-20 Eukaryotic DNA topoisomerase I, catalytic core
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 205, 307
AclWI GGATC 2 cut(s) 423, 436
AcsI RAATTY 3 cut(s) 54, 62, 116
AcuI CTGAAG 3 cut(s) 371, 461, 523
AfaI GTAC 2 cut(s) 39, 85
AfiI CCNNNNNNNGG 3 cut(s) 26, 66, 96
AgsI TTSAA 2 cut(s) 94, 139
AjuI GAANNNNNNNTTGG 2 cut(s) 496, 528
AluBI AGCT 5 cut(s) 155, 211, 385, 453, 475
AluI AGCT 5 cut(s) 155, 211, 385, 453, 475
Alw21I GWGCWC 2 cut(s) 352, 442
Alw44I GTGCAC 2 cut(s) 348, 438
AlwI GGATC 2 cut(s) 423, 436
AlwNI CAGNNNCTG 1 cut(s) 546
Ama87I CYCGRG 1 cut(s) 174
AoxI GGCC 1 cut(s) 529
ApaLI GTGCAC 2 cut(s) 348, 438
ApeKI GCWGC 2 cut(s) 164, 208
ApoI RAATTY 3 cut(s) 54, 62, 116
AspLEI GCGC 1 cut(s) 417
AspS9I GGNCC 2 cut(s) 29, 530
AsuHPI GGTGA 2 cut(s) 392, 482
AsuNHI GCTAGC 1 cut(s) 211
AvaI CYCGRG 1 cut(s) 174
AvaII GGWCC 1 cut(s) 29
BaeGI GKGCMC 2 cut(s) 352, 442
BamHI GGATCC 1 cut(s) 428
Bbv12I GWGCWC 2 cut(s) 352, 442
BbvCI CCTCAGC 1 cut(s) 5
BbvI GCAGC 2 cut(s) 151, 220
BccI CCATC 1 cut(s) 188
BfaI CTAG 4 cut(s) 51, 74, 212, 360
BfmI CTRYAG 1 cut(s) 433
BisI GCNGC 4 cut(s) 165, 206, 209, 307
BlsI GCNGC 4 cut(s) 166, 207, 210, 308
BmcAI AGTACT 2 cut(s) 39, 85
Bme18I GGWCC 1 cut(s) 29
BmeT110I CYCGRG 1 cut(s) 174
BmgT120I GGNCC 2 cut(s) 29, 530
BmiI GGNNCC 1 cut(s) 430
BmtI GCTAGC 1 cut(s) 215
BpmI CTGGAG 1 cut(s) 18
Bpu10I CCTNAGC 1 cut(s) 5
BpuEI CTTGAG 1 cut(s) 520
Bsc4I CCNNNNNNNGG 3 cut(s) 26, 66, 96
Bse1I ACTGG 1 cut(s) 35
Bse3DI GCAATG 1 cut(s) 525
BseGI GGATG 3 cut(s) 199, 283, 529
BseLI CCNNNNNNNGG 3 cut(s) 26, 66, 96
BseMI GCAATG 1 cut(s) 525
BseMII CTCAG 1 cut(s) 252
BseNI ACTGG 1 cut(s) 35
BseSI GKGCMC 2 cut(s) 352, 442
BseXI GCAGC 2 cut(s) 151, 220
BshFI GGCC 1 cut(s) 531
BsiHKAI GWGCWC 2 cut(s) 352, 442
BsiHKCI CYCGRG 1 cut(s) 174
BslFI GGGAC 1 cut(s) 478
BslI CCNNNNNNNGG 3 cut(s) 26, 66, 96
BsmFI GGGAC 1 cut(s) 478
BsnI GGCC 1 cut(s) 531
BsoBI CYCGRG 1 cut(s) 174
Bsp1286I GDGCHC 2 cut(s) 352, 442
Bsp143I GATC 2 cut(s) 428, 465
BspACI CCGC 2 cut(s) 205, 307
BspANI GGCC 1 cut(s) 531
BspCNI CTCAG 1 cut(s) 253
BspLI GGNNCC 1 cut(s) 430
BspOI GCTAGC 1 cut(s) 215
BspPI GGATC 2 cut(s) 423, 436
BspQI GCTCTTC 1 cut(s) 330
BsrDI GCAATG 1 cut(s) 525
BsrI ACTGG 1 cut(s) 35
BssMI GATC 2 cut(s) 428, 465
Bst6I CTCTTC 2 cut(s) 89, 330
BstC8I GCNNGC 1 cut(s) 213
BstDEI CTNAG 5 cut(s) 5, 41, 80, 248, 261
BstENI CCTNNNNNAGG 1 cut(s) 94
BstF5I GGATG 3 cut(s) 199, 283, 529
BstHHI GCGC 1 cut(s) 417
BstKTI GATC 2 cut(s) 431, 468
BstMBI GATC 2 cut(s) 428, 465
BstMWI GCNNNNNNNGC 1 cut(s) 161
BstSFI CTRYAG 1 cut(s) 433
BstSLI GKGCMC 2 cut(s) 352, 442
BstV1I GCAGC 2 cut(s) 151, 220
BstX2I RGATCY 1 cut(s) 428
BstYI RGATCY 1 cut(s) 428
BsuRI GGCC 1 cut(s) 531
BtsCI GGATG 3 cut(s) 199, 283, 529
BtsI GCAGTG 1 cut(s) 222
BtsIMutI CAGTG 1 cut(s) 222
Cac8I GCNNGC 1 cut(s) 213
CaiI CAGNNNCTG 1 cut(s) 546
CfoI GCGC 1 cut(s) 417
Cfr13I GGNCC 2 cut(s) 29, 530
Csp6I GTAC 2 cut(s) 38, 84
CviAII CATG 1 cut(s) 232
CviQI GTAC 2 cut(s) 38, 84
DdeI CTNAG 5 cut(s) 5, 41, 80, 248, 261
DpnI GATC 2 cut(s) 430, 467
DpnII GATC 2 cut(s) 428, 465
Eam1104I CTCTTC 2 cut(s) 89, 330
EarI CTCTTC 2 cut(s) 89, 330
Eco32I GATATC 2 cut(s) 331, 397
Eco47I GGWCC 1 cut(s) 29
Eco57I CTGAAG 3 cut(s) 371, 461, 523
Eco88I CYCGRG 1 cut(s) 174
EcoNI CCTNNNNNAGG 1 cut(s) 94
EcoRV GATATC 2 cut(s) 331, 397
FaeI CATG 1 cut(s) 235
FaiI YATR 6 cut(s) 149, 169, 233, 345, 411, 435
FalI AAGNNNNNCTT 2 cut(s) 482, 514
FaqI GGGAC 1 cut(s) 478
FatI CATG 1 cut(s) 231
Fnu4HI GCNGC 4 cut(s) 165, 206, 209, 307
FokI GGATG 3 cut(s) 206, 290, 536
Fsp4HI GCNGC 4 cut(s) 165, 206, 209, 307
FspBI CTAG 4 cut(s) 51, 74, 212, 360
GlaI GCGC 1 cut(s) 416
GluI GCNGC 4 cut(s) 165, 206, 209, 307
GsuI CTGGAG 1 cut(s) 18
HaeIII GGCC 1 cut(s) 531
HhaI GCGC 1 cut(s) 417
Hin1II CATG 1 cut(s) 235
Hin6I GCGC 1 cut(s) 415
HinP1I GCGC 1 cut(s) 415
HinfI GANTC 1 cut(s) 543
HphI GGTGA 2 cut(s) 392, 482
Hpy166II GTNNAC 2 cut(s) 350, 440
Hpy188I TCNGA 2 cut(s) 542, 548
Hpy188III TCNNGA 4 cut(s) 27, 51, 176, 499
Hpy8I GTNNAC 2 cut(s) 350, 440
HpyAV CCTTC 2 cut(s) 133, 259
HpyCH4IV ACGT 1 cut(s) 134
HpyCH4V TGCA 4 cut(s) 167, 185, 350, 440
HpyF10VI GCNNNNNNNGC 1 cut(s) 161
HpyF3I CTNAG 5 cut(s) 5, 41, 80, 248, 261
HpySE526I ACGT 1 cut(s) 134
Hsp92II CATG 1 cut(s) 235
HspAI GCGC 1 cut(s) 415
Kzo9I GATC 2 cut(s) 428, 465
LguI GCTCTTC 1 cut(s) 330
LmnI GCTCC 1 cut(s) 152
LpnPI CCDG 7 cut(s) 12, 48, 145, 240, 374, 411, 464
Lsp1109I GCAGC 2 cut(s) 151, 220
MaeI CTAG 4 cut(s) 51, 74, 212, 360
MaeII ACGT 1 cut(s) 134
MaeIII GTNAC 1 cut(s) 104
MalI GATC 2 cut(s) 430, 467
MboI GATC 2 cut(s) 428, 465
MboII GAAGA 4 cut(s) 106, 347, 385, 475
MflI RGATCY 1 cut(s) 428
MhlI GDGCHC 2 cut(s) 352, 442
MluCI AATT 4 cut(s) 54, 62, 116, 443
MnlI CCTC 7 cut(s) 34, 42, 90, 184, 268, 351, 506
MseI TTAA 2 cut(s) 314, 455
MslI CAYNNNNRTG 1 cut(s) 324
MwoI GCNNNNNNNGC 1 cut(s) 161
NdeII GATC 2 cut(s) 428, 465
NheI GCTAGC 1 cut(s) 211
NlaIII CATG 1 cut(s) 235
NlaIV GGNNCC 1 cut(s) 430
NmuCI GTSAC 1 cut(s) 104
PciSI GCTCTTC 1 cut(s) 330
PfeI GAWTC 1 cut(s) 543
PkrI GCNGC 4 cut(s) 166, 207, 210, 308
PspN4I GGNNCC 1 cut(s) 430
PspPI GGNCC 2 cut(s) 29, 530
PstNI CAGNNNCTG 1 cut(s) 546
PsuI RGATCY 1 cut(s) 428
RsaI GTAC 2 cut(s) 39, 85
RsaNI GTAC 2 cut(s) 38, 84
RseI CAYNNNNRTG 1 cut(s) 324
SapI GCTCTTC 1 cut(s) 330
SaqAI TTAA 2 cut(s) 314, 455
SatI GCNGC 4 cut(s) 165, 206, 209, 307
Sau3AI GATC 2 cut(s) 428, 465
Sau96I GGNCC 2 cut(s) 29, 530
ScaI AGTACT 2 cut(s) 39, 85
SduI GDGCHC 2 cut(s) 352, 442
SfcI CTRYAG 1 cut(s) 433
SinI GGWCC 1 cut(s) 29
SmiMI CAYNNNNRTG 1 cut(s) 324
SmlI CTYRAG 1 cut(s) 499
SmoI CTYRAG 1 cut(s) 499
Sse9I AATT 4 cut(s) 54, 62, 116, 443
SsiI CCGC 2 cut(s) 205, 307
SspMI CTAG 4 cut(s) 51, 74, 212, 360
TaiI ACGT 1 cut(s) 137
TaqI TCGA 1 cut(s) 333
TasI AATT 4 cut(s) 54, 62, 116, 443
TatI WGTACW 2 cut(s) 37, 83
TauI GCSGC 2 cut(s) 208, 309
TfiI GAWTC 1 cut(s) 543
Tru1I TTAA 2 cut(s) 314, 455
Tru9I TTAA 2 cut(s) 314, 455
TscAI CASTG 1 cut(s) 222
TseFI GTSAC 1 cut(s) 104
TseI GCWGC 2 cut(s) 164, 208
Tsp45I GTSAC 1 cut(s) 104
TspDTI ATGAA 1 cut(s) 300
TspRI CASTG 1 cut(s) 222
VneI GTGCAC 2 cut(s) 348, 438
VpaK11BI GGWCC 1 cut(s) 29
XagI CCTNNNNNAGG 1 cut(s) 94
XapI RAATTY 3 cut(s) 54, 62, 116
XbaI TCTAGA 1 cut(s) 50
XspI CTAG 4 cut(s) 51, 74, 212, 360
ZrmI AGTACT 2 cut(s) 39, 85
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.