Rh4AG223700

DNA topoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0007552
Physical Location & Seq
Forward (+)
52320770 .. 52342649
21880 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG223700.1

Sequence Viewer

Length: 1596 bp
ATGGGGAATGATGCGCACGACAGTGGTTTTATTAGGGCTGAGGACTGGCAGCCTGGAATTACAAGGATTACTGAGGTGCTACCAAAATGGACTTCCGGCGAGGGGTGGAGCCCTGACGGTGGTGGAGGCGTGTTGGCAGATACCAGTGGTTCTGGCTTCGCTAAAGTCGACACCTTGGTGGCTGGTGGTTTCATTTGTGAGGGCGGCGTCCCTGGTGGTCGTTTTGATAGCGATCGGCATGGCAGGGAGGACAACAATGCCGGGCTCGTTTTGGGCAATAGAAACTTCGGCGGCGGCTGCGAGGGGTTCCAGTTGACTGCTAGGTTTTCTAGCAGAGAACGAAAACCTGCACACCAGGCCAATTCAGTTCTTGTTGACGTACGTCCTAACAGAATTCTGAGTTCCGCTTTAAAATGCTTGTTGATGGTGAATAGCTCTGCCCATTACTTCTATCGTGAACTTGACTTATGGTGTGAGGTATCAGCCAAAATGCTGCATTTTACTGATGATAGGCAGCAAAGATGGACTAGTTTAGTGCACAATGGTTTCCTTTTCCCACCTCCATACAAGCCTCATGGGGTAAACATGCTTTACAATGGGAGGCCAGTCAACTTGACTCCTGAACAAGAGGAGGAGAAGAAAGCCGTCAAGCAAGAGGACTTGAAACAACAGCTTAAGTATAAGTGGGCTCTCGTTGGTGGTGTCAGAAAAGAGGTTATCTTTAGCTTTGATATTTTTCACTTTTACTTGGCATCACCTGGGTTATTCCGAGGACGTGGGGAGCACCCAAAGTCAGGAAAGCTTGTAAGGTGGATTTCTCCAAAAGATGTTACAATCAATACAGGAAAGGGTGCCCTGATTCCAGAATGTCCAATCCGTGGTGAAAGCTGGAAAGAAATAAGGCATGACAATACTATGACATGTACTTCCTTGAAGAGGCAAAGTGACGAGGAGAAATTTGAGAAAGCAAGGAAGTTGAAGGACTACATAGGAGCTATCAGGGCTGCATACACTTGGGATATTACAAGTGAGGATGGTCAAAAGCGGCAGCTAGCAGTGGCTACCTATCTCATGGATAAACTTGGTCTGAGGGCTGGAACTGAGAAGGTTGATGAGGATGATGATGAAGCTGATATTGTCAGTTGCCGCAGATTAAAAGTAGAGAATGTAGAAGCAATATCCCCTATCTTGTTAGAGTTTAGCTTCCTCAGTAAAGATTTAATCAGATATAAAAATGCTGTTGAAGTTGATCTTCCTGTTTATGAGGCAATTGTAATGTTTCAGGCTGGTAAGTGTAGCAGTGACGAACTGTTTGACAAGTGGGACACCCTTGAGCAAGGGGACTATAGAGGGAGATGTTTCAGAGAAACTGATGTTTATGCCCGTGCAAACAAGGAGGTTGCTATCACTTGTAAGCATCTGTGCAGTATCTCAAAGACTGATAGTGAACAAACCTCTAGGTTAATGCTAAAGATTTACAAGCTCCTGGACATTTTGACAGAGCTGAAAGCATACTTGGACGGGTATAAGAAGAAGAGTCCCCAGCGAGAGATTCTTATGGAAAACGAAAGAGTGATTCGGCTCGAGAAGAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Topo_C_assoc Topoisom_I Topoisom_I_N

Protein Analysis

531

Amino Acids

60.1

Weight (kDa)

7.15

Isoelectric Point (pI)

37.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Topoisom_I_N PF02919 173 - 211 5.5e-10 Eukaryotic DNA topoisomerase I, DNA binding fragment
Topoisom_I_N PF02919 210 - 307 3.1e-24 Eukaryotic DNA topoisomerase I, DNA binding fragment
Topoisom_I PF01028 310 - 443 5.9e-37 Eukaryotic DNA topoisomerase I, catalytic core
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 15
Acc36I ACCTGC 1 cut(s) 355
AccB1I GGYRCC 1 cut(s) 851
AccB7I CCANNNNNTGG 1 cut(s) 878
AccI GTMKAC 1 cut(s) 168
AciI CCGC 6 cut(s) 204, 291, 294, 405, 1045, 1147
AcsI RAATTY 2 cut(s) 393, 956
AcyI GRCGYC 1 cut(s) 207
AfaI GTAC 2 cut(s) 381, 925
AfiI CCNNNNNNNGG 5 cut(s) 102, 119, 794, 878, 936
AflII CTTAAG 1 cut(s) 674
AflIII ACRYGT 1 cut(s) 920
AgsI TTSAA 4 cut(s) 664, 934, 979, 1244
AhlI ACTAGT 1 cut(s) 527
AjiI CACGTC 1 cut(s) 776
AjnI CCWGG 5 cut(s) 52, 211, 354, 757, 1485
AjuI GAANNNNNNNTTGG 4 cut(s) 76, 108, 1499, 1531
AleI CACNNNNGTG 2 cut(s) 21, 176
Alw21I GWGCWC 2 cut(s) 540, 786
Alw44I GTGCAC 1 cut(s) 536
Ama87I CYCGRG 1 cut(s) 1583
AoxI GGCC 2 cut(s) 357, 602
ApaLI GTGCAC 1 cut(s) 536
ApeKI GCWGC 6 cut(s) 49, 297, 493, 514, 1004, 1048
ApoI RAATTY 2 cut(s) 393, 956
ArsI GACNNNNNNTTYG 2 cut(s) 1295, 1327
AspLEI GCGC 1 cut(s) 16
AsuC2I CCSGG 1 cut(s) 262
AsuHPI GGTGA 3 cut(s) 439, 747, 893
AsuNHI GCTAGC 1 cut(s) 1051
AvaI CYCGRG 1 cut(s) 1583
BaeGI GKGCMC 2 cut(s) 540, 856
BanI GGYRCC 1 cut(s) 851
BanII GRGCYC 3 cut(s) 113, 267, 691
Bbv12I GWGCWC 2 cut(s) 540, 786
BbvCI CCTCAGC 1 cut(s) 39
BbvI GCAGC 6 cut(s) 61, 284, 480, 526, 991, 1060
BccI CCATC 3 cut(s) 418, 516, 1028
BceAI ACGGC 1 cut(s) 629
BciT130I CCWGG 5 cut(s) 54, 213, 356, 759, 1487
BcnI CCSGG 1 cut(s) 262
BcuI ACTAGT 1 cut(s) 527
BfaI CTAG 5 cut(s) 321, 330, 528, 1052, 1458
BfmI CTRYAG 1 cut(s) 1345
BfrI CTTAAG 1 cut(s) 674
BfuAI ACCTGC 1 cut(s) 355
Bme1390I CCNGG 6 cut(s) 54, 213, 262, 356, 759, 1487
BmeT110I CYCGRG 1 cut(s) 1583
BmgBI CACGTC 1 cut(s) 776
BmiI GGNNCC 3 cut(s) 110, 308, 853
BmrFI CCNGG 6 cut(s) 54, 213, 262, 356, 759, 1487
BmsI GCATC 2 cut(s) 761, 1426
BmtI GCTAGC 1 cut(s) 1055
BoxI GACNNNNGTC 1 cut(s) 381
Bpu10I CCTNAGC 1 cut(s) 39
BpuEI CTTGAG 1 cut(s) 1352
BpuMI CCSGG 1 cut(s) 262
BsaBI GATNNNNATC 1 cut(s) 231
BsaHI GRCGYC 1 cut(s) 207
BsaJI CCNNGG 5 cut(s) 174, 211, 758, 769, 877
Bsc4I CCNNNNNNNGG 5 cut(s) 102, 119, 794, 878, 936
Bse1I ACTGG 4 cut(s) 50, 144, 310, 605
Bse8I GATNNNNATC 1 cut(s) 231
BseBI CCWGG 5 cut(s) 54, 213, 356, 759, 1487
BseDI CCNNGG 5 cut(s) 174, 211, 758, 769, 877
BseGI GGATG 2 cut(s) 1039, 1123
BseJI GATNNNNATC 1 cut(s) 231
BseLI CCNNNNNNNGG 5 cut(s) 102, 119, 794, 878, 936
BseMII CTCAG 6 cut(s) 30, 63, 389, 1079, 1092, 1222
BseNI ACTGG 4 cut(s) 50, 144, 310, 605
BseRI GAGGAG 3 cut(s) 644, 647, 965
BseSI GKGCMC 2 cut(s) 540, 856
BseXI GCAGC 6 cut(s) 61, 284, 480, 526, 991, 1060
BseYI CCCAGC 1 cut(s) 1542
BsgI GTGCAG 2 cut(s) 333, 1444
Bsh1285I CGRYCG 1 cut(s) 235
BshFI GGCC 2 cut(s) 359, 604
BshNI GGYRCC 1 cut(s) 851
BsiEI CGRYCG 1 cut(s) 235
BsiHKAI GWGCWC 2 cut(s) 540, 786
BsiHKCI CYCGRG 1 cut(s) 1583
BsiSI CCGG 2 cut(s) 96, 261
BsiWI CGTACG 1 cut(s) 379
BslFI GGGAC 4 cut(s) 194, 1337, 1355, 1524
BslI CCNNNNNNNGG 5 cut(s) 102, 119, 794, 878, 936
BsmFI GGGAC 4 cut(s) 194, 1337, 1355, 1524
BsnI GGCC 2 cut(s) 359, 604
BsoBI CYCGRG 1 cut(s) 1583
Bsp1286I GDGCHC 6 cut(s) 113, 267, 540, 691, 786, 856
Bsp143I GATC 2 cut(s) 232, 1249
BspACI CCGC 6 cut(s) 204, 291, 294, 405, 1045, 1147
BspANI GGCC 2 cut(s) 359, 604
BspCNI CTCAG 6 cut(s) 31, 64, 390, 1080, 1093, 1221
BspLI GGNNCC 3 cut(s) 110, 308, 853
BspMI ACCTGC 1 cut(s) 355
BspOI GCTAGC 1 cut(s) 1055
BspT107I GGYRCC 1 cut(s) 851
BspTI CTTAAG 1 cut(s) 674
BsrI ACTGG 4 cut(s) 50, 144, 310, 605
BssECI CCNNGG 5 cut(s) 174, 211, 758, 769, 877
BssMI GATC 2 cut(s) 232, 1249
BssNI GRCGYC 1 cut(s) 207
BssT1I CCWWGG 1 cut(s) 174
Bst2UI CCWGG 5 cut(s) 54, 213, 356, 759, 1487
Bst4CI ACNGT 3 cut(s) 23, 119, 1311
Bst6I CTCTTC 3 cut(s) 929, 1529, 1583
BstACI GRCGYC 1 cut(s) 207
BstAFI CTTAAG 1 cut(s) 674
BstC8I GCNNGC 1 cut(s) 1053
BstDEI CTNAG 6 cut(s) 39, 72, 398, 1088, 1101, 1208
BstDSI CCRYGG 1 cut(s) 877
BstENI CCTNNNNNAGG 1 cut(s) 934
BstF5I GGATG 2 cut(s) 1039, 1123
BstHHI GCGC 1 cut(s) 16
BstKTI GATC 2 cut(s) 235, 1252
BstMBI GATC 2 cut(s) 232, 1249
BstMCI CGRYCG 1 cut(s) 235
BstMWI GCNNNNNNNGC 3 cut(s) 297, 356, 1001
BstNI CCWGG 5 cut(s) 54, 213, 356, 759, 1487
BstNSI RCATGY 2 cut(s) 589, 924
BstPAI GACNNNNGTC 1 cut(s) 381
BstSCI CCNGG 6 cut(s) 52, 211, 260, 354, 757, 1485
BstSFI CTRYAG 1 cut(s) 1345
BstSLI GKGCMC 2 cut(s) 540, 856
BstV1I GCAGC 6 cut(s) 61, 284, 480, 526, 991, 1060
BsuRI GGCC 2 cut(s) 359, 604
BtgI CCRYGG 1 cut(s) 877
BtrI CACGTC 1 cut(s) 776
BtsCI GGATG 2 cut(s) 1039, 1123
BtsI GCAGTG 2 cut(s) 1062, 1306
BtsIMutI CAGTG 4 cut(s) 28, 151, 1062, 1306
BveI ACCTGC 1 cut(s) 355
Cac8I GCNNGC 1 cut(s) 1053
CfoI GCGC 1 cut(s) 16
CseI GACGC 1 cut(s) 196
Csp6I GTAC 2 cut(s) 380, 924
CviAII CATG 6 cut(s) 239, 575, 586, 905, 921, 1072
CviQI GTAC 2 cut(s) 380, 924
DdeI CTNAG 6 cut(s) 39, 72, 398, 1088, 1101, 1208
DpnI GATC 2 cut(s) 234, 1251
DpnII GATC 2 cut(s) 232, 1249
DraI TTTAAA 1 cut(s) 411
Eam1104I CTCTTC 3 cut(s) 929, 1529, 1583
EarI CTCTTC 3 cut(s) 929, 1529, 1583
Eco130I CCWWGG 1 cut(s) 174
Eco24I GRGCYC 3 cut(s) 113, 267, 691
Eco88I CYCGRG 1 cut(s) 1583
EcoNI CCTNNNNNAGG 1 cut(s) 934
EcoRI GAATTC 1 cut(s) 393
EcoRII CCWGG 5 cut(s) 52, 211, 354, 757, 1485
EcoT14I CCWWGG 1 cut(s) 174
EcoT38I GRGCYC 3 cut(s) 113, 267, 691
ErhI CCWWGG 1 cut(s) 174
FaeI CATG 6 cut(s) 242, 578, 589, 908, 924, 1075
FalI AAGNNNNNCTT 2 cut(s) 1236, 1268
FaqI GGGAC 4 cut(s) 194, 1337, 1355, 1524
FatI CATG 6 cut(s) 238, 574, 585, 904, 920, 1071
FblI GTMKAC 1 cut(s) 168
FokI GGATG 2 cut(s) 1046, 1130
FriOI GRGCYC 3 cut(s) 113, 267, 691
FspAI RTGCGCAY 1 cut(s) 15
FspBI CTAG 5 cut(s) 321, 330, 528, 1052, 1458
FspI TGCGCA 1 cut(s) 15
GlaI GCGC 1 cut(s) 15
GsaI CCCAGC 1 cut(s) 1546
HaeIII GGCC 2 cut(s) 359, 604
HapII CCGG 2 cut(s) 96, 261
HgaI GACGC 1 cut(s) 196
HhaI GCGC 1 cut(s) 16
Hin1I GRCGYC 1 cut(s) 207
Hin1II CATG 6 cut(s) 242, 578, 589, 908, 924, 1075
Hin6I GCGC 1 cut(s) 14
HinP1I GCGC 1 cut(s) 14
HincII GTYRAC 4 cut(s) 169, 315, 376, 610
HindII GTYRAC 4 cut(s) 169, 315, 376, 610
HindIII AAGCTT 1 cut(s) 800
HinfI GANTC 5 cut(s) 616, 859, 1537, 1552, 1576
HpaII CCGG 2 cut(s) 96, 261
HphI GGTGA 3 cut(s) 439, 747, 893
Hpy166II GTNNAC 8 cut(s) 169, 315, 376, 458, 538, 583, 610, 1448
Hpy188I TCNGA 6 cut(s) 399, 707, 770, 1089, 1226, 1364
Hpy188III TCNNGA 5 cut(s) 455, 620, 795, 863, 1585
Hpy8I GTNNAC 8 cut(s) 169, 315, 376, 458, 538, 583, 610, 1448
HpyAV CCTTC 2 cut(s) 973, 1099
HpyCH4III ACNGT 3 cut(s) 23, 119, 1311
HpyCH4IV ACGT 3 cut(s) 378, 382, 775
HpyCH4V TGCA 6 cut(s) 350, 496, 538, 1007, 1388, 1425
HpyF10VI GCNNNNNNNGC 3 cut(s) 297, 356, 1001
HpyF3I CTNAG 6 cut(s) 39, 72, 398, 1088, 1101, 1208
HpySE526I ACGT 3 cut(s) 378, 382, 775
Hsp92I GRCGYC 1 cut(s) 207
Hsp92II CATG 6 cut(s) 242, 578, 589, 908, 924, 1075
HspAI GCGC 1 cut(s) 14
Kzo9I GATC 2 cut(s) 232, 1249
LmnI GCTCC 4 cut(s) 108, 781, 992, 1488
Lsp1109I GCAGC 6 cut(s) 61, 284, 480, 526, 991, 1060
LweI GCATC 2 cut(s) 761, 1426
MaeI CTAG 5 cut(s) 321, 330, 528, 1052, 1458
MaeII ACGT 3 cut(s) 378, 382, 775
MaeIII GTNAC 3 cut(s) 829, 944, 1301
MalI GATC 2 cut(s) 234, 1251
MboI GATC 2 cut(s) 232, 1249
MboII GAAGA 5 cut(s) 649, 946, 1244, 1543, 1546
MfeI CAATTG 1 cut(s) 1269
MhlI GDGCHC 6 cut(s) 113, 267, 540, 691, 786, 856
MluCI AATT 5 cut(s) 57, 361, 393, 956, 1269
MlyI GAGTC 2 cut(s) 610, 1546
MseI TTAA 5 cut(s) 410, 675, 1154, 1220, 1463
MslI CAYNNNNRTG 2 cut(s) 21, 176
MspCI CTTAAG 1 cut(s) 674
MspI CCGG 2 cut(s) 96, 261
MspR9I CCNGG 6 cut(s) 54, 213, 262, 356, 759, 1487
MunI CAATTG 1 cut(s) 1269
MvaI CCWGG 5 cut(s) 54, 213, 356, 759, 1487
MwoI GCNNNNNNNGC 3 cut(s) 297, 356, 1001
NciI CCSGG 1 cut(s) 262
NdeII GATC 2 cut(s) 232, 1249
NheI GCTAGC 1 cut(s) 1051
NlaIII CATG 6 cut(s) 242, 578, 589, 908, 924, 1075
NlaIV GGNNCC 3 cut(s) 110, 308, 853
NmuCI GTSAC 2 cut(s) 944, 1301
NsbI TGCGCA 1 cut(s) 15
NspI RCATGY 2 cut(s) 589, 924
OliI CACNNNNGTG 2 cut(s) 21, 176
PaeR7I CTCGAG 1 cut(s) 1583
PciI ACATGT 1 cut(s) 920
PcsI WCGNNNNNNNCGW 1 cut(s) 165
PfeI GAWTC 3 cut(s) 859, 1552, 1576
Pfl23II CGTACG 1 cut(s) 379
PflMI CCANNNNNTGG 1 cut(s) 878
PfoI TCCNGGA 1 cut(s) 1485
Ple19I CGATCG 1 cut(s) 235
PleI GAGTC 2 cut(s) 610, 1545
PpsI GAGTC 2 cut(s) 610, 1545
PscI ACATGT 1 cut(s) 920
PshAI GACNNNNGTC 1 cut(s) 381
Psp6I CCWGG 5 cut(s) 52, 211, 354, 757, 1485
PspFI CCCAGC 1 cut(s) 1542
PspGI CCWGG 5 cut(s) 52, 211, 354, 757, 1485
PspLI CGTACG 1 cut(s) 379
PspN4I GGNNCC 3 cut(s) 110, 308, 853
PvuI CGATCG 1 cut(s) 235
RsaI GTAC 2 cut(s) 381, 925
RsaNI GTAC 2 cut(s) 380, 924
RseI CAYNNNNRTG 2 cut(s) 21, 176
SalI GTCGAC 1 cut(s) 167
SaqAI TTAA 5 cut(s) 410, 675, 1154, 1220, 1463
Sau3AI GATC 2 cut(s) 232, 1249
SchI GAGTC 2 cut(s) 610, 1546
ScrFI CCNGG 6 cut(s) 54, 213, 262, 356, 759, 1487
SduI GDGCHC 6 cut(s) 113, 267, 540, 691, 786, 856
SfaNI GCATC 2 cut(s) 761, 1426
SfcI CTRYAG 1 cut(s) 1345
Sfr274I CTCGAG 1 cut(s) 1583
SlaI CTCGAG 1 cut(s) 1583
SmiMI CAYNNNNRTG 2 cut(s) 21, 176
SmlI CTYRAG 3 cut(s) 674, 1331, 1583
SmoI CTYRAG 3 cut(s) 674, 1331, 1583
SpeI ACTAGT 1 cut(s) 527
Sse9I AATT 5 cut(s) 57, 361, 393, 956, 1269
SsiI CCGC 6 cut(s) 204, 291, 294, 405, 1045, 1147
SspMI CTAG 5 cut(s) 321, 330, 528, 1052, 1458
StyD4I CCNGG 6 cut(s) 52, 211, 260, 354, 757, 1485
StyI CCWWGG 1 cut(s) 174
TaaI ACNGT 3 cut(s) 23, 119, 1311
TaiI ACGT 3 cut(s) 381, 385, 778
TaqI TCGA 2 cut(s) 168, 1584
TasI AATT 5 cut(s) 57, 361, 393, 956, 1269
TatI WGTACW 1 cut(s) 923
TauI GCSGC 5 cut(s) 207, 294, 297, 1048, 1149
TfiI GAWTC 3 cut(s) 859, 1552, 1576
Tru1I TTAA 5 cut(s) 410, 675, 1154, 1220, 1463
Tru9I TTAA 5 cut(s) 410, 675, 1154, 1220, 1463
TscAI CASTG 4 cut(s) 28, 151, 1062, 1306
TseFI GTSAC 2 cut(s) 944, 1301
TseI GCWGC 6 cut(s) 49, 297, 493, 514, 1004, 1048
Tsp45I GTSAC 2 cut(s) 944, 1301
TspDTI ATGAA 2 cut(s) 181, 1140
TspGWI ACGGA 1 cut(s) 866
TspRI CASTG 4 cut(s) 28, 151, 1062, 1306
Van91I CCANNNNNTGG 1 cut(s) 878
Vha464I CTTAAG 1 cut(s) 674
VneI GTGCAC 1 cut(s) 536
XagI CCTNNNNNAGG 1 cut(s) 934
XapI RAATTY 2 cut(s) 393, 956
XceI RCATGY 2 cut(s) 589, 924
XhoI CTCGAG 1 cut(s) 1583
XmiI GTMKAC 1 cut(s) 168
XspI CTAG 5 cut(s) 321, 330, 528, 1052, 1458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.