RLG00000009409

DNA topoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0007552
Physical Location & Seq
Forward (+)
52486419 .. 52488476
2058 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000009409

Sequence Viewer

Length: 567 bp
ATGGATAAACTTGGTCTAAGGTCTGGAACTGAGAAGGTTGATGAGGATGATGATGAAACTGATATTGTCAGTTGCCGCAGATTAAAAGTAGAGAATGTAAAAGCAATATCCCCTATCTTGTTAGAGTTTAGCTTCCTCGGTAAAGATTTAATCAGATATAAAAATGCTATTGAAGTTGATCTTCCTGTTTATGAGGCAATTGTAATATTTCAGGCTGGTAAGTGTAGCAGTGACGAACTGTTTGACAAGTTGGACACCCATAAATTGAATGCACATTTAAAGGCACTGATGTCCGGCCTTACAGTGGAAGTATTGCATGTAGCCGTGCATACAATTCATCAGCGTGATATCGAAGAGCCTCATAGTGCACAAATGTCTAGGATAATGGAGAAGATTGGTGAGCTACAGGTTGATATCATCTCTAATCATAAGCGTAATATCACAGGATCCTATAGTGCACAATTTGATAGCTTAATGGAGAAGATCGGTGAGCTTCAGGTATTTCCAAAGTCCCTCTTGCGAAAGTTTGCTTGGGCAATGGATGTCGGCCCTGACTTCAAATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Topo_C_assoc Topoisom_I Topoisom_I_N

Protein Analysis

189

Amino Acids

21.34

Weight (kDa)

5.5

Isoelectric Point (pI)

37.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Topoisom_I PF01028 1 - 166 1.2e-29 Eukaryotic DNA topoisomerase I, catalytic core
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 76
AclWI GGATC 2 cut(s) 441, 454
AcsI RAATTY 1 cut(s) 560
AcuI CTGAAG 1 cut(s) 479
AfiI CCNNNNNNNGG 1 cut(s) 304
AgsI TTSAA 3 cut(s) 173, 268, 559
AjuI GAANNNNNNNTTGG 2 cut(s) 514, 546
AluBI AGCT 4 cut(s) 132, 403, 471, 493
AluI AGCT 4 cut(s) 132, 403, 471, 493
Alw21I GWGCWC 2 cut(s) 370, 460
Alw44I GTGCAC 2 cut(s) 366, 456
AlwI GGATC 2 cut(s) 441, 454
AoxI GGCC 2 cut(s) 295, 547
ApaLI GTGCAC 2 cut(s) 366, 456
ApoI RAATTY 1 cut(s) 560
ArsI GACNNNNNNTTYG 2 cut(s) 224, 256
AspS9I GGNCC 1 cut(s) 548
AsuHPI GGTGA 2 cut(s) 410, 500
BaeGI GKGCMC 2 cut(s) 370, 460
BamHI GGATCC 1 cut(s) 446
Bbv12I GWGCWC 2 cut(s) 370, 460
BceAI ACGGC 1 cut(s) 308
BfaI CTAG 1 cut(s) 378
BfmI CTRYAG 2 cut(s) 404, 451
BisI GCNGC 1 cut(s) 76
BlsI GCNGC 1 cut(s) 77
BmgT120I GGNCC 1 cut(s) 548
BmiI GGNNCC 1 cut(s) 448
BsaJI CCNNGG 1 cut(s) 136
Bsc4I CCNNNNNNNGG 1 cut(s) 304
Bse3DI GCAATG 1 cut(s) 543
BseDI CCNNGG 1 cut(s) 136
BseGI GGATG 2 cut(s) 52, 547
BseLI CCNNNNNNNGG 1 cut(s) 304
BseMI GCAATG 1 cut(s) 543
BseMII CTCAG 1 cut(s) 21
BseSI GKGCMC 2 cut(s) 370, 460
BshFI GGCC 2 cut(s) 297, 549
BsiHKAI GWGCWC 2 cut(s) 370, 460
BsiSI CCGG 1 cut(s) 294
BslFI GGGAC 1 cut(s) 496
BslI CCNNNNNNNGG 1 cut(s) 304
BsmFI GGGAC 1 cut(s) 496
BsmI GAATGC 1 cut(s) 274
BsnI GGCC 2 cut(s) 297, 549
Bsp1286I GDGCHC 2 cut(s) 370, 460
Bsp143I GATC 3 cut(s) 178, 446, 483
BspACI CCGC 1 cut(s) 76
BspANI GGCC 2 cut(s) 297, 549
BspCNI CTCAG 1 cut(s) 22
BspLI GGNNCC 1 cut(s) 448
BspPI GGATC 2 cut(s) 441, 454
BspQI GCTCTTC 1 cut(s) 348
BsrDI GCAATG 1 cut(s) 543
BssECI CCNNGG 1 cut(s) 136
BssMI GATC 3 cut(s) 178, 446, 483
Bst4CI ACNGT 2 cut(s) 240, 304
Bst6I CTCTTC 1 cut(s) 348
BstDEI CTNAG 2 cut(s) 17, 30
BstF5I GGATG 2 cut(s) 52, 547
BstKTI GATC 3 cut(s) 181, 449, 486
BstMBI GATC 3 cut(s) 178, 446, 483
BstNSI RCATGY 1 cut(s) 320
BstSFI CTRYAG 2 cut(s) 404, 451
BstSLI GKGCMC 2 cut(s) 370, 460
BstX2I RGATCY 1 cut(s) 446
BstYI RGATCY 1 cut(s) 446
BsuRI GGCC 2 cut(s) 297, 549
BtsCI GGATG 2 cut(s) 52, 547
BtsI GCAGTG 1 cut(s) 235
BtsIMutI CAGTG 3 cut(s) 235, 284, 309
Cfr13I GGNCC 1 cut(s) 548
CviAII CATG 1 cut(s) 317
CviJI RGCY 9 cut(s) 132, 215, 297, 323, 358, 403, 471, 493, 549
CviKI_1 RGCY 9 cut(s) 132, 215, 297, 323, 358, 403, 471, 493, 549
DdeI CTNAG 2 cut(s) 17, 30
DpnI GATC 3 cut(s) 180, 448, 485
DpnII GATC 3 cut(s) 178, 446, 483
DraI TTTAAA 1 cut(s) 279
Eam1104I CTCTTC 1 cut(s) 348
EarI CTCTTC 1 cut(s) 348
Eco32I GATATC 2 cut(s) 349, 415
Eco57I CTGAAG 1 cut(s) 479
EcoRV GATATC 2 cut(s) 349, 415
FaeI CATG 1 cut(s) 320
FaiI YATR 8 cut(s) 159, 192, 261, 318, 330, 363, 429, 453
FalI AAGNNNNNCTT 4 cut(s) 165, 197, 500, 532
FaqI GGGAC 1 cut(s) 496
FatI CATG 1 cut(s) 316
Fnu4HI GCNGC 1 cut(s) 76
FokI GGATG 2 cut(s) 59, 554
Fsp4HI GCNGC 1 cut(s) 76
FspBI CTAG 1 cut(s) 378
GluI GCNGC 1 cut(s) 76
HaeIII GGCC 2 cut(s) 297, 549
HapII CCGG 1 cut(s) 294
Hin1II CATG 1 cut(s) 320
HpaII CCGG 1 cut(s) 294
HphI GGTGA 2 cut(s) 410, 500
Hpy166II GTNNAC 2 cut(s) 368, 458
Hpy188I TCNGA 2 cut(s) 155, 566
Hpy188III TCNNGA 1 cut(s) 24
Hpy8I GTNNAC 2 cut(s) 368, 458
HpyAV CCTTC 1 cut(s) 28
HpyCH4III ACNGT 2 cut(s) 240, 304
HpyCH4V TGCA 5 cut(s) 272, 316, 328, 368, 458
HpyF3I CTNAG 2 cut(s) 17, 30
Hsp92II CATG 1 cut(s) 320
Kzo9I GATC 3 cut(s) 178, 446, 483
LguI GCTCTTC 1 cut(s) 348
LpnPI CCDG 8 cut(s) 9, 197, 198, 201, 307, 392, 429, 482
MaeI CTAG 1 cut(s) 378
MaeIII GTNAC 1 cut(s) 230
MalI GATC 3 cut(s) 180, 448, 485
MboI GATC 3 cut(s) 178, 446, 483
MboII GAAGA 4 cut(s) 173, 365, 403, 493
MfeI CAATTG 1 cut(s) 198
MflI RGATCY 1 cut(s) 446
MhlI GDGCHC 2 cut(s) 370, 460
MluCI AATT 5 cut(s) 198, 263, 333, 461, 560
MmeI TCCRAC 1 cut(s) 231
MnlI CCTC 5 cut(s) 37, 146, 187, 369, 524
MseI TTAA 4 cut(s) 83, 149, 278, 473
MslI CAYNNNNRTG 1 cut(s) 342
MspI CCGG 1 cut(s) 294
MunI CAATTG 1 cut(s) 198
Mva1269I GAATGC 1 cut(s) 274
NdeII GATC 3 cut(s) 178, 446, 483
NlaIII CATG 1 cut(s) 320
NlaIV GGNNCC 1 cut(s) 448
NmuCI GTSAC 1 cut(s) 230
NspI RCATGY 1 cut(s) 320
PciSI GCTCTTC 1 cut(s) 348
PctI GAATGC 1 cut(s) 274
PkrI GCNGC 1 cut(s) 77
PspN4I GGNNCC 1 cut(s) 448
PspPI GGNCC 1 cut(s) 548
PsuI RGATCY 1 cut(s) 446
RseI CAYNNNNRTG 1 cut(s) 342
SapI GCTCTTC 1 cut(s) 348
SaqAI TTAA 4 cut(s) 83, 149, 278, 473
SatI GCNGC 1 cut(s) 76
Sau3AI GATC 3 cut(s) 178, 446, 483
Sau96I GGNCC 1 cut(s) 548
SduI GDGCHC 2 cut(s) 370, 460
SetI ASST 8 cut(s) 23, 39, 134, 405, 411, 473, 495, 501
SfcI CTRYAG 2 cut(s) 404, 451
SmiMI CAYNNNNRTG 1 cut(s) 342
Sse9I AATT 5 cut(s) 198, 263, 333, 461, 560
SsiI CCGC 1 cut(s) 76
SspI AATATT 1 cut(s) 207
SspMI CTAG 1 cut(s) 378
TaaI ACNGT 2 cut(s) 240, 304
TaqI TCGA 1 cut(s) 351
TasI AATT 5 cut(s) 198, 263, 333, 461, 560
TauI GCSGC 1 cut(s) 78
Tru1I TTAA 4 cut(s) 83, 149, 278, 473
Tru9I TTAA 4 cut(s) 83, 149, 278, 473
TscAI CASTG 3 cut(s) 235, 291, 309
TseFI GTSAC 1 cut(s) 230
Tsp45I GTSAC 1 cut(s) 230
TspDTI ATGAA 2 cut(s) 69, 326
TspRI CASTG 3 cut(s) 235, 291, 309
VneI GTGCAC 2 cut(s) 366, 456
XapI RAATTY 1 cut(s) 560
XceI RCATGY 1 cut(s) 320
XspI CTAG 1 cut(s) 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.