Rh4CG233600

DNA topoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0007552
Physical Location & Seq
Forward (+)
47520156 .. 47522769
2614 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG233600.1

Sequence Viewer

Length: 660 bp
ATGTTTCTAGCAGGACCTCCAGTACTTAGAACTTCTGGAATTTCCAAATTTGGGTATCTAGCACTTAGTACTTCCTTGAAGAGGCAAAGTGACAAGGAAAAATTTGAGAAAGCAAGGACGTTGAAGGACTACATAGGAGCTATCAGGGCTGCCTACACTCGGGATATTACAAGTGAGGATGGTCAAAAGCGGCAGCTAGCAATGGCTGCAGATCTCATGGATAAACTTGGTCTGAGGGCTGGAACTGAGAAGGTTGATGAGGATGATGATGAAACTGATATTGTCAGTTGCCGCAGATTAAAAGTAGAGAATGTAAAAGCAATATCCCCTATCTTGTTAGAGTTGAACAAGAAAACTATCGAGGGTGATATATCAGCAACCAAGGAGGTGAAAGTTGAAATGGTTTGTAGTCATCAGCGTGATATCGAAAAGCGTCATAGTGCACAAATGTCTAGGATAATGGAGAAGATTGGTGAGCTTCAGGTTGATATCATCTTTAATCATAAGCGCAATATCACAGGATCCTATAGTGCACAATTTGATAGCTTAATGGAGAAGATATCGGTGAGCTTCAGACTTGCAGATATATGGGTACAAAATGGCACGTGGGCAGAGCATTTAGGACTTGTTGAAGAATGTTTCAATCAGCCAGGGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Topo_C_assoc Topoisom_I Topoisom_I_N

Protein Analysis

219

Amino Acids

24.68

Weight (kDa)

6.46

Isoelectric Point (pI)

40.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Topoisom_I PF01028 24 - 110 5.2e-21 Eukaryotic DNA topoisomerase I, catalytic core
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 190, 292
AclWI GGATC 2 cut(s) 516, 529
AcsI RAATTY 3 cut(s) 39, 47, 101
AcuI CTGAAG 2 cut(s) 464, 556
AcvI CACGTG 1 cut(s) 606
AfaI GTAC 3 cut(s) 24, 70, 594
AfiI CCNNNNNNNGG 3 cut(s) 51, 81, 159
AgsI TTSAA 6 cut(s) 79, 124, 346, 398, 632, 643
AjnI CCWGG 1 cut(s) 649
AluBI AGCT 5 cut(s) 140, 196, 478, 546, 570
AluI AGCT 5 cut(s) 140, 196, 478, 546, 570
Alw21I GWGCWC 2 cut(s) 445, 535
Alw44I GTGCAC 2 cut(s) 441, 531
AlwI GGATC 2 cut(s) 516, 529
Ama87I CYCGRG 1 cut(s) 159
ApaLI GTGCAC 2 cut(s) 441, 531
ApeKI GCWGC 3 cut(s) 149, 193, 206
ApoI RAATTY 3 cut(s) 39, 47, 101
AspLEI GCGC 1 cut(s) 510
AspS9I GGNCC 1 cut(s) 14
AsuHPI GGTGA 4 cut(s) 377, 400, 485, 577
AsuNHI GCTAGC 1 cut(s) 196
AvaI CYCGRG 1 cut(s) 159
AvaII GGWCC 1 cut(s) 14
BaeGI GKGCMC 2 cut(s) 445, 535
BamHI GGATCC 1 cut(s) 521
BbrPI CACGTG 1 cut(s) 606
Bbv12I GWGCWC 2 cut(s) 445, 535
BbvI GCAGC 3 cut(s) 136, 193, 205
BccI CCATC 1 cut(s) 173
BciT130I CCWGG 1 cut(s) 651
BfaI CTAG 4 cut(s) 8, 59, 197, 453
BfmI CTRYAG 2 cut(s) 207, 526
BglII AGATCT 1 cut(s) 211
BisI GCNGC 5 cut(s) 150, 191, 194, 207, 292
BlsI GCNGC 5 cut(s) 151, 192, 195, 208, 293
BmcAI AGTACT 2 cut(s) 24, 70
Bme1390I CCNGG 1 cut(s) 651
Bme18I GGWCC 1 cut(s) 14
BmeT110I CYCGRG 1 cut(s) 159
BmgT120I GGNCC 1 cut(s) 14
BmiI GGNNCC 1 cut(s) 523
BmrFI CCNGG 1 cut(s) 651
BmtI GCTAGC 1 cut(s) 200
BsaAI YACGTR 1 cut(s) 606
BsaJI CCNNGG 2 cut(s) 381, 650
Bsc4I CCNNNNNNNGG 3 cut(s) 51, 81, 159
Bse1I ACTGG 1 cut(s) 20
Bse3DI GCAATG 1 cut(s) 207
BseBI CCWGG 1 cut(s) 651
BseDI CCNNGG 2 cut(s) 381, 650
BseGI GGATG 2 cut(s) 184, 268
BseLI CCNNNNNNNGG 3 cut(s) 51, 81, 159
BseMI GCAATG 1 cut(s) 207
BseMII CTCAG 2 cut(s) 224, 237
BseNI ACTGG 1 cut(s) 20
BseSI GKGCMC 2 cut(s) 445, 535
BseXI GCAGC 3 cut(s) 136, 193, 205
BsiHKAI GWGCWC 2 cut(s) 445, 535
BsiHKCI CYCGRG 1 cut(s) 159
BslI CCNNNNNNNGG 3 cut(s) 51, 81, 159
BsoBI CYCGRG 1 cut(s) 159
Bsp1286I GDGCHC 2 cut(s) 445, 535
Bsp143I GATC 2 cut(s) 211, 521
BspACI CCGC 2 cut(s) 190, 292
BspCNI CTCAG 2 cut(s) 225, 238
BspLI GGNNCC 1 cut(s) 523
BspMAI CTGCAG 1 cut(s) 211
BspOI GCTAGC 1 cut(s) 200
BspPI GGATC 2 cut(s) 516, 529
BsrDI GCAATG 1 cut(s) 207
BsrI ACTGG 1 cut(s) 20
BssECI CCNNGG 2 cut(s) 381, 650
BssMI GATC 2 cut(s) 211, 521
BssT1I CCWWGG 1 cut(s) 381
Bst2UI CCWGG 1 cut(s) 651
Bst6I CTCTTC 1 cut(s) 74
BstAPI GCANNNNNTGC 1 cut(s) 206
BstBAI YACGTR 1 cut(s) 606
BstC8I GCNNGC 1 cut(s) 198
BstDEI CTNAG 4 cut(s) 26, 65, 233, 246
BstENI CCTNNNNNAGG 1 cut(s) 79
BstF5I GGATG 2 cut(s) 184, 268
BstHHI GCGC 1 cut(s) 510
BstKTI GATC 2 cut(s) 214, 524
BstMBI GATC 2 cut(s) 211, 521
BstMWI GCNNNNNNNGC 2 cut(s) 146, 206
BstNI CCWGG 1 cut(s) 651
BstSCI CCNGG 1 cut(s) 649
BstSFI CTRYAG 2 cut(s) 207, 526
BstSLI GKGCMC 2 cut(s) 445, 535
BstV1I GCAGC 3 cut(s) 136, 193, 205
BstX2I RGATCY 2 cut(s) 211, 521
BstYI RGATCY 2 cut(s) 211, 521
BtsCI GGATG 2 cut(s) 184, 268
Cac8I GCNNGC 1 cut(s) 198
CfoI GCGC 1 cut(s) 510
Cfr13I GGNCC 1 cut(s) 14
CseI GACGC 1 cut(s) 422
Csp6I GTAC 3 cut(s) 23, 69, 593
CviAII CATG 1 cut(s) 217
CviJI RGCY 9 cut(s) 140, 149, 196, 206, 239, 478, 546, 570, 649
CviKI_1 RGCY 9 cut(s) 140, 149, 196, 206, 239, 478, 546, 570, 649
CviQI GTAC 3 cut(s) 23, 69, 593
DdeI CTNAG 4 cut(s) 26, 65, 233, 246
DpnI GATC 2 cut(s) 213, 523
DpnII GATC 2 cut(s) 211, 521
Eam1104I CTCTTC 1 cut(s) 74
EarI CTCTTC 1 cut(s) 74
Eco130I CCWWGG 1 cut(s) 381
Eco32I GATATC 3 cut(s) 424, 490, 561
Eco47I GGWCC 1 cut(s) 14
Eco57I CTGAAG 2 cut(s) 464, 556
Eco72I CACGTG 1 cut(s) 606
Eco88I CYCGRG 1 cut(s) 159
EcoNI CCTNNNNNAGG 1 cut(s) 79
EcoO109I RGGNCCY 1 cut(s) 14
EcoRII CCWGG 1 cut(s) 649
EcoRV GATATC 3 cut(s) 424, 490, 561
EcoT14I CCWWGG 1 cut(s) 381
ErhI CCWWGG 1 cut(s) 381
FaeI CATG 1 cut(s) 220
FaiI YATR 8 cut(s) 134, 218, 371, 438, 504, 528, 587, 589
FatI CATG 1 cut(s) 216
Fnu4HI GCNGC 5 cut(s) 150, 191, 194, 207, 292
FokI GGATG 2 cut(s) 191, 275
Fsp4HI GCNGC 5 cut(s) 150, 191, 194, 207, 292
FspBI CTAG 4 cut(s) 8, 59, 197, 453
GlaI GCGC 1 cut(s) 509
GluI GCNGC 5 cut(s) 150, 191, 194, 207, 292
HgaI GACGC 1 cut(s) 422
HhaI GCGC 1 cut(s) 510
Hin1II CATG 1 cut(s) 220
Hin6I GCGC 1 cut(s) 508
HinP1I GCGC 1 cut(s) 508
HphI GGTGA 4 cut(s) 377, 400, 485, 577
Hpy166II GTNNAC 2 cut(s) 443, 533
Hpy188I TCNGA 2 cut(s) 234, 575
Hpy188III TCNNGA 3 cut(s) 36, 161, 657
Hpy8I GTNNAC 2 cut(s) 443, 533
HpyAV CCTTC 2 cut(s) 118, 244
HpyCH4IV ACGT 2 cut(s) 119, 605
HpyCH4V TGCA 4 cut(s) 209, 443, 533, 581
HpyF10VI GCNNNNNNNGC 2 cut(s) 146, 206
HpyF3I CTNAG 4 cut(s) 26, 65, 233, 246
HpySE526I ACGT 2 cut(s) 119, 605
Hsp92II CATG 1 cut(s) 220
HspAI GCGC 1 cut(s) 508
Kzo9I GATC 2 cut(s) 211, 521
LmnI GCTCC 1 cut(s) 137
LpnPI CCDG 7 cut(s) 21, 33, 130, 225, 467, 504, 636
Lsp1109I GCAGC 3 cut(s) 136, 193, 205
MaeI CTAG 4 cut(s) 8, 59, 197, 453
MaeII ACGT 2 cut(s) 119, 605
MaeIII GTNAC 1 cut(s) 89
MalI GATC 2 cut(s) 213, 523
MboI GATC 2 cut(s) 211, 521
MboII GAAGA 4 cut(s) 91, 478, 568, 644
MflI RGATCY 2 cut(s) 211, 521
MhlI GDGCHC 2 cut(s) 445, 535
MluCI AATT 4 cut(s) 39, 47, 101, 536
MnlI CCTC 7 cut(s) 27, 75, 169, 228, 253, 355, 379
MseI TTAA 3 cut(s) 299, 498, 548
MslI CAYNNNNRTG 1 cut(s) 417
MspR9I CCNGG 1 cut(s) 651
MvaI CCWGG 1 cut(s) 651
MwoI GCNNNNNNNGC 2 cut(s) 146, 206
NdeII GATC 2 cut(s) 211, 521
NheI GCTAGC 1 cut(s) 196
NlaIII CATG 1 cut(s) 220
NlaIV GGNNCC 1 cut(s) 523
NmuCI GTSAC 1 cut(s) 89
PkrI GCNGC 5 cut(s) 151, 192, 195, 208, 293
PmaCI CACGTG 1 cut(s) 606
PmlI CACGTG 1 cut(s) 606
Ppu21I YACGTR 1 cut(s) 606
PpuMI RGGWCCY 1 cut(s) 14
Psp5II RGGWCCY 1 cut(s) 14
Psp6I CCWGG 1 cut(s) 649
PspCI CACGTG 1 cut(s) 606
PspGI CCWGG 1 cut(s) 649
PspN4I GGNNCC 1 cut(s) 523
PspPI GGNCC 1 cut(s) 14
PspPPI RGGWCCY 1 cut(s) 14
PstI CTGCAG 1 cut(s) 211
PsuI RGATCY 2 cut(s) 211, 521
RsaI GTAC 3 cut(s) 24, 70, 594
RsaNI GTAC 3 cut(s) 23, 69, 593
RseI CAYNNNNRTG 1 cut(s) 417
SaqAI TTAA 3 cut(s) 299, 498, 548
SatI GCNGC 5 cut(s) 150, 191, 194, 207, 292
Sau3AI GATC 2 cut(s) 211, 521
Sau96I GGNCC 1 cut(s) 14
ScaI AGTACT 2 cut(s) 24, 70
ScrFI CCNGG 1 cut(s) 651
SduI GDGCHC 2 cut(s) 445, 535
SfcI CTRYAG 2 cut(s) 207, 526
SinI GGWCC 1 cut(s) 14
SmiMI CAYNNNNRTG 1 cut(s) 417
Sse9I AATT 4 cut(s) 39, 47, 101, 536
SsiI CCGC 2 cut(s) 190, 292
SspMI CTAG 4 cut(s) 8, 59, 197, 453
StyD4I CCNGG 1 cut(s) 649
StyI CCWWGG 1 cut(s) 381
TaiI ACGT 2 cut(s) 122, 608
TaqI TCGA 2 cut(s) 360, 426
TasI AATT 4 cut(s) 39, 47, 101, 536
TatI WGTACW 2 cut(s) 22, 68
TauI GCSGC 2 cut(s) 193, 294
Tru1I TTAA 3 cut(s) 299, 498, 548
Tru9I TTAA 3 cut(s) 299, 498, 548
TseFI GTSAC 1 cut(s) 89
TseI GCWGC 3 cut(s) 149, 193, 206
Tsp45I GTSAC 1 cut(s) 89
TspDTI ATGAA 1 cut(s) 285
VneI GTGCAC 2 cut(s) 441, 531
VpaK11BI GGWCC 1 cut(s) 14
XagI CCTNNNNNAGG 1 cut(s) 79
XapI RAATTY 3 cut(s) 39, 47, 101
XspI CTAG 4 cut(s) 8, 59, 197, 453
ZrmI AGTACT 2 cut(s) 24, 70
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.