Rh2DG202300

DNA topoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0007552
Physical Location & Seq
Reverse (-)
18667004 .. 18674614
7611 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG202300.1

Sequence Viewer

Length: 762 bp
ATGGCTGCTACCAGCAATATGGGTCGAGCTCCACTACTCCTCCTCCCTCCACTTGGTCTTCTTGGGCGTGTTGGTCTTTGGTGTCCTCACCGAGGAAGCACCGATTCCCTATTCGACCTTCCTCAGATGAGACTACTGTTTTTACTCAATTTGGCCATCGAGAAGGCCAAGTATGTCTTCCCCAAGCAGTTCGAGGCTCGGACTCTTGAGGAAGCTCTCATGTCAGGACCTCCAGTACTTAGAACTTCTGGAATTTCCAAATTTAGGTATCTAGCACTTAGTACTTCCTTGAAGAGGCAAAGTGACAAGGAGAAATTTGAGAAAGCAAGGACGTTGAAGGACTACATAGGAGCTATCAGGGCTGCATACACTCGGGATATTACAAGTGAGGATGGTCAAAAGCGGCAGCCTATCTCATGGATAAACTTGGTCAAAGGGCTGGAACTGAGAAGGTTGATGAGGATGATGATGAAACTGATATTGTCAGTTGCCGCAGAGAATGTAAAAGCAATAGCCCCTATCTTGTTAGAGTTGAACAAGAAAACTATCGAGGGTGATATATCAGCAACCAAGGAGGTTGAAATGGTTTGTAGTCATCAGCGTGATATCAAAGAGCCTCATAGTGCACAAATGTCTAGGATAATGGAGAAGATTGGTGAGCTTCAGGTTGATAGCATCTCTAATCATAAGCGCAATATCACAGGATCCTATAGTGCACAATTTGATAGCTTAATGGAGAAGATCGGTGAGCTTCAGGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Topo_C_assoc Topoisom_I Topoisom_I_N

Protein Analysis

253

Amino Acids

28.53

Weight (kDa)

9.62

Isoelectric Point (pI)

45.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Topoisom_I PF01028 95 - 150 4.2e-09 Eukaryotic DNA topoisomerase I, catalytic core
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 403, 492
AclWI GGATC 2 cut(s) 699, 712
AcoI YGGCCR 1 cut(s) 153
AcsI RAATTY 3 cut(s) 252, 260, 314
AcuI CTGAAG 2 cut(s) 647, 737
AfaI GTAC 2 cut(s) 237, 283
AfiI CCNNNNNNNGG 4 cut(s) 53, 92, 264, 294
AgsI TTSAA 4 cut(s) 292, 337, 535, 581
AjuI GAANNNNNNNTTGG 2 cut(s) 161, 193
AluBI AGCT 6 cut(s) 29, 215, 353, 661, 729, 751
AluI AGCT 6 cut(s) 29, 215, 353, 661, 729, 751
Alw21I GWGCWC 3 cut(s) 31, 628, 718
Alw26I GTCTC 1 cut(s) 124
Alw44I GTGCAC 2 cut(s) 624, 714
AlwI GGATC 2 cut(s) 699, 712
Ama87I CYCGRG 1 cut(s) 372
AoxI GGCC 2 cut(s) 153, 165
ApaLI GTGCAC 2 cut(s) 624, 714
ApeKI GCWGC 3 cut(s) 5, 362, 406
ApoI RAATTY 3 cut(s) 252, 260, 314
AspLEI GCGC 1 cut(s) 693
AspS9I GGNCC 1 cut(s) 227
AsuHPI GGTGA 4 cut(s) 80, 566, 668, 758
AvaI CYCGRG 1 cut(s) 372
AvaII GGWCC 1 cut(s) 227
BaeGI GKGCMC 2 cut(s) 628, 718
BalI TGGCCA 1 cut(s) 155
BamHI GGATCC 1 cut(s) 704
BanII GRGCYC 1 cut(s) 31
BbsI GAAGAC 2 cut(s) 50, 169
Bbv12I GWGCWC 3 cut(s) 31, 628, 718
BbvI GCAGC 2 cut(s) 349, 418
BccI CCATC 2 cut(s) 164, 386
BcoDI GTCTC 1 cut(s) 124
BfaI CTAG 2 cut(s) 272, 636
BfmI CTRYAG 1 cut(s) 709
BisI GCNGC 5 cut(s) 6, 363, 404, 407, 492
BlsI GCNGC 5 cut(s) 7, 364, 405, 408, 493
BmcAI AGTACT 2 cut(s) 237, 283
Bme18I GGWCC 1 cut(s) 227
BmeT110I CYCGRG 1 cut(s) 372
BmgT120I GGNCC 1 cut(s) 227
BmiI GGNNCC 1 cut(s) 706
BmsI GCATC 1 cut(s) 684
BpiI GAAGAC 2 cut(s) 50, 169
BpmI CTGGAG 1 cut(s) 216
BpuEI CTTGAG 1 cut(s) 227
BsaJI CCNNGG 2 cut(s) 91, 570
BsaXI ACNNNNNCTCC 4 cut(s) 24, 27, 54, 57
Bsc4I CCNNNNNNNGG 4 cut(s) 53, 92, 264, 294
Bse1I ACTGG 1 cut(s) 233
BseDI CCNNGG 2 cut(s) 91, 570
BseGI GGATG 2 cut(s) 397, 468
BseLI CCNNNNNNNGG 4 cut(s) 53, 92, 264, 294
BseMII CTCAG 2 cut(s) 137, 437
BseNI ACTGG 1 cut(s) 233
BseRI GAGGAG 2 cut(s) 29, 32
BseSI GKGCMC 2 cut(s) 628, 718
BseXI GCAGC 2 cut(s) 349, 418
BshFI GGCC 2 cut(s) 155, 167
BsiHKAI GWGCWC 3 cut(s) 31, 628, 718
BsiHKCI CYCGRG 1 cut(s) 372
BslI CCNNNNNNNGG 4 cut(s) 53, 92, 264, 294
BsmAI GTCTC 1 cut(s) 124
BsnI GGCC 2 cut(s) 155, 167
BsoBI CYCGRG 1 cut(s) 372
Bsp1286I GDGCHC 3 cut(s) 31, 628, 718
Bsp143I GATC 2 cut(s) 704, 741
BspACI CCGC 2 cut(s) 403, 492
BspANI GGCC 2 cut(s) 155, 167
BspCNI CTCAG 2 cut(s) 136, 438
BspLI GGNNCC 1 cut(s) 706
BspPI GGATC 2 cut(s) 699, 712
BsrI ACTGG 1 cut(s) 233
BssECI CCNNGG 2 cut(s) 91, 570
BssMI GATC 2 cut(s) 704, 741
BssT1I CCWWGG 1 cut(s) 570
Bst4CI ACNGT 1 cut(s) 138
Bst6I CTCTTC 1 cut(s) 287
BstDEI CTNAG 4 cut(s) 123, 239, 278, 446
BstENI CCTNNNNNAGG 2 cut(s) 90, 292
BstF5I GGATG 2 cut(s) 397, 468
BstHHI GCGC 1 cut(s) 693
BstKTI GATC 2 cut(s) 707, 744
BstMAI GTCTC 1 cut(s) 124
BstMBI GATC 2 cut(s) 704, 741
BstMWI GCNNNNNNNGC 1 cut(s) 359
BstSFI CTRYAG 1 cut(s) 709
BstSLI GKGCMC 2 cut(s) 628, 718
BstV1I GCAGC 2 cut(s) 349, 418
BstV2I GAAGAC 2 cut(s) 50, 169
BstX2I RGATCY 1 cut(s) 704
BstXI CCANNNNNNTGG 1 cut(s) 19
BstYI RGATCY 1 cut(s) 704
BsuRI GGCC 2 cut(s) 155, 167
BtsCI GGATG 2 cut(s) 397, 468
CfoI GCGC 1 cut(s) 693
Cfr13I GGNCC 1 cut(s) 227
Csp6I GTAC 2 cut(s) 236, 282
CviAII CATG 2 cut(s) 220, 417
CviQI GTAC 2 cut(s) 236, 282
DdeI CTNAG 4 cut(s) 123, 239, 278, 446
DpnI GATC 2 cut(s) 706, 743
DpnII GATC 2 cut(s) 704, 741
EaeI YGGCCR 1 cut(s) 153
Eam1104I CTCTTC 1 cut(s) 287
EarI CTCTTC 1 cut(s) 287
Ecl136II GAGCTC 1 cut(s) 29
Eco130I CCWWGG 1 cut(s) 570
Eco24I GRGCYC 1 cut(s) 31
Eco32I GATATC 1 cut(s) 607
Eco47I GGWCC 1 cut(s) 227
Eco53kI GAGCTC 1 cut(s) 29
Eco57I CTGAAG 2 cut(s) 647, 737
Eco88I CYCGRG 1 cut(s) 372
EcoICRI GAGCTC 1 cut(s) 29
EcoNI CCTNNNNNAGG 2 cut(s) 90, 292
EcoO109I RGGNCCY 1 cut(s) 227
EcoRV GATATC 1 cut(s) 607
EcoT14I CCWWGG 1 cut(s) 570
EcoT38I GRGCYC 1 cut(s) 31
ErhI CCWWGG 1 cut(s) 570
FaeI CATG 2 cut(s) 223, 420
FalI AAGNNNNNCTT 2 cut(s) 161, 193
FatI CATG 2 cut(s) 219, 416
Fnu4HI GCNGC 5 cut(s) 6, 363, 404, 407, 492
FokI GGATG 2 cut(s) 404, 475
FriOI GRGCYC 1 cut(s) 31
Fsp4HI GCNGC 5 cut(s) 6, 363, 404, 407, 492
FspBI CTAG 2 cut(s) 272, 636
GlaI GCGC 1 cut(s) 692
GluI GCNGC 5 cut(s) 6, 363, 404, 407, 492
GsuI CTGGAG 1 cut(s) 216
HaeIII GGCC 2 cut(s) 155, 167
HhaI GCGC 1 cut(s) 693
Hin1II CATG 2 cut(s) 223, 420
Hin6I GCGC 1 cut(s) 691
HinP1I GCGC 1 cut(s) 691
HinfI GANTC 2 cut(s) 104, 202
HphI GGTGA 4 cut(s) 80, 566, 668, 758
Hpy166II GTNNAC 2 cut(s) 626, 716
Hpy188I TCNGA 2 cut(s) 126, 201
Hpy188III TCNNGA 5 cut(s) 160, 206, 225, 249, 374
Hpy8I GTNNAC 2 cut(s) 626, 716
HpyAV CCTTC 4 cut(s) 128, 157, 331, 444
HpyCH4III ACNGT 1 cut(s) 138
HpyCH4IV ACGT 1 cut(s) 332
HpyCH4V TGCA 3 cut(s) 365, 626, 716
HpyF10VI GCNNNNNNNGC 1 cut(s) 359
HpyF3I CTNAG 4 cut(s) 123, 239, 278, 446
HpySE526I ACGT 1 cut(s) 332
Hsp92II CATG 2 cut(s) 223, 420
HspAI GCGC 1 cut(s) 691
Kzo9I GATC 2 cut(s) 704, 741
LmnI GCTCC 2 cut(s) 34, 350
LpnPI CCDG 9 cut(s) 25, 210, 234, 246, 343, 425, 650, 687, 740
Lsp1109I GCAGC 2 cut(s) 349, 418
LweI GCATC 1 cut(s) 684
MaeI CTAG 2 cut(s) 272, 636
MaeII ACGT 1 cut(s) 332
MaeIII GTNAC 1 cut(s) 302
MalI GATC 2 cut(s) 706, 743
MboI GATC 2 cut(s) 704, 741
MboII GAAGA 5 cut(s) 50, 169, 304, 661, 751
MflI RGATCY 1 cut(s) 704
MhlI GDGCHC 3 cut(s) 31, 628, 718
MlsI TGGCCA 1 cut(s) 155
MluCI AATT 5 cut(s) 148, 252, 260, 314, 719
MluNI TGGCCA 1 cut(s) 155
MlyI GAGTC 1 cut(s) 196
Mox20I TGGCCA 1 cut(s) 155
MscI TGGCCA 1 cut(s) 155
MseI TTAA 1 cut(s) 731
MslI CAYNNNNRTG 1 cut(s) 600
Msp20I TGGCCA 1 cut(s) 155
MwoI GCNNNNNNNGC 1 cut(s) 359
NdeII GATC 2 cut(s) 704, 741
NlaIII CATG 2 cut(s) 223, 420
NlaIV GGNNCC 1 cut(s) 706
NmuCI GTSAC 1 cut(s) 302
PfeI GAWTC 1 cut(s) 104
PkrI GCNGC 5 cut(s) 7, 364, 405, 408, 493
PleI GAGTC 1 cut(s) 196
PpsI GAGTC 1 cut(s) 196
PpuMI RGGWCCY 1 cut(s) 227
Psp124BI GAGCTC 1 cut(s) 31
Psp5II RGGWCCY 1 cut(s) 227
PspN4I GGNNCC 1 cut(s) 706
PspPI GGNCC 1 cut(s) 227
PspPPI RGGWCCY 1 cut(s) 227
PsuI RGATCY 1 cut(s) 704
RsaI GTAC 2 cut(s) 237, 283
RsaNI GTAC 2 cut(s) 236, 282
RseI CAYNNNNRTG 1 cut(s) 600
SacI GAGCTC 1 cut(s) 31
SaqAI TTAA 1 cut(s) 731
SatI GCNGC 5 cut(s) 6, 363, 404, 407, 492
Sau3AI GATC 2 cut(s) 704, 741
Sau96I GGNCC 1 cut(s) 227
ScaI AGTACT 2 cut(s) 237, 283
SchI GAGTC 1 cut(s) 196
SduI GDGCHC 3 cut(s) 31, 628, 718
SfaNI GCATC 1 cut(s) 684
SfcI CTRYAG 1 cut(s) 709
SinI GGWCC 1 cut(s) 227
SmiMI CAYNNNNRTG 1 cut(s) 600
SmlI CTYRAG 1 cut(s) 206
SmoI CTYRAG 1 cut(s) 206
Sse9I AATT 5 cut(s) 148, 252, 260, 314, 719
SsiI CCGC 2 cut(s) 403, 492
SspMI CTAG 2 cut(s) 272, 636
SstI GAGCTC 1 cut(s) 31
StyI CCWWGG 1 cut(s) 570
TaaI ACNGT 1 cut(s) 138
TaiI ACGT 1 cut(s) 335
TaqI TCGA 5 cut(s) 25, 114, 159, 192, 549
TasI AATT 5 cut(s) 148, 252, 260, 314, 719
TatI WGTACW 2 cut(s) 235, 281
TauI GCSGC 2 cut(s) 406, 494
TfiI GAWTC 1 cut(s) 104
Tru1I TTAA 1 cut(s) 731
Tru9I TTAA 1 cut(s) 731
TseFI GTSAC 1 cut(s) 302
TseI GCWGC 3 cut(s) 5, 362, 406
Tsp45I GTSAC 1 cut(s) 302
TspDTI ATGAA 1 cut(s) 485
VneI GTGCAC 2 cut(s) 624, 714
VpaK11BI GGWCC 1 cut(s) 227
XagI CCTNNNNNAGG 2 cut(s) 90, 292
XapI RAATTY 3 cut(s) 252, 260, 314
XspI CTAG 2 cut(s) 272, 636
ZrmI AGTACT 2 cut(s) 237, 283
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.